CN106084081A - The preparation method of polysaccharide component and products application in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel. - Google Patents
The preparation method of polysaccharide component and products application in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel. Download PDFInfo
- Publication number
- CN106084081A CN106084081A CN201610426470.3A CN201610426470A CN106084081A CN 106084081 A CN106084081 A CN 106084081A CN 201610426470 A CN201610426470 A CN 201610426470A CN 106084081 A CN106084081 A CN 106084081A
- Authority
- CN
- China
- Prior art keywords
- quel
- betula
- polysaccharide component
- phellinus igniarius
- mountainrange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Sustainable Development (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel., it comprises the following steps: pretreatment；Prepare extracting solution, supersound process, obtain sonification fluid；Add compound enzyme, obtain enzymolysis solution；Microwave treatment, obtains microwave treatment liquid；Centrifuge is centrifuged, and takes the supernatant after being centrifuged；Ethanol, and precipitate with ethanol 10 20h is added in supernatant；Centrifugal, precipitation；Precipitation is dissolved in a small amount of water, lyophilization, obtains the polysaccharide component present invention and can reduce the extraction cost of polysaccharide component, improve extraction ratio, use the extracting method of the present invention, extraction ratio can be higher than 75 mg/g, and the method is easily controllable, simple to operate, and utilize the method that ultrasonic assistant, microwave-assisted combine, substantially increasing extraction efficiency, safe, quick, energy-conservation, extraction time is short.
Description
Technical field
The invention belongs to extracting method technical field, especially relate in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel.
The preparation method of polysaccharide component and products application, belong to field of natural organic chemistry.
Background technology
In Daxing'an Mountainrange, containing substantial amounts of urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel., urtica angustifolia be a conventional ethnic drug and
Folk medicine, begins to be loaded in " figure is through book on Chinese herbal medicine ", has the effects such as dispelling wind and removing obstruction in the collateral, suppressing the hyperactive liver arresting convulsion, removing food stagnancy relieving constipation, removing toxic substances, urtica angustifolia
Take in nineteen ninety-five " the Sanitation Ministry medicine standard " (Tibetan medicine) first.Abroad, urtica angustifolia since cist generation just
Medicinal, it is widely used in treatment rheumatic illness and some Senile disease, evident in efficacy, polysaccharide is antiinflammatory, anticancer main
One of active component.Pharmacological research also confirms that, polysaccharide from urtica fissa truly has significant antiinflammatory, enhancing immunization to grind the most both at home and abroad
Studying carefully and show, polysaccharide has an important activity effect at aspects such as antitumor, immunomodulating, defying age, blood sugar lowering, antiviral, and birch
Tree Phellinus igniarius (L. ex Fr.) Quel. is China's one Chinese medicine material, belongs to Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, phellinus
Belonging to, main parasitic is on the trunks such as mulberry, willow, robur.The most many researchs show, extract many in Phellinus igniarius (L. ex Fr.) Quel. sporophore
Sugar composition has obvious antitumaous effect, improves the functions such as immunity of organisms simultaneously.
Therefore, how to extract the polysaccharide component in urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel., for urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel.
Utilize, have a very important role and meaning, for the development of medicine, there is good impetus.
Summary of the invention
The present invention is directed to existing technical problem, it is provided that polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel.
Preparation method and products application, can reduce the extraction cost of polysaccharide component, improve extraction ratio, use the extraction side of the present invention
Method, extraction ratio can be higher than 75 mg/g, and the method is easily controllable, simple to operate, and utilizes ultrasonic assistant, microwave-assisted phase
In conjunction with method, substantially increase extraction efficiency, safe, quick, energy-conservation, extraction time is short.
For achieving the above object, the present invention provides following technical scheme: a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel.
The preparation method of middle polysaccharide component, it is characterised in that it comprises the following steps:
(1) urtica angustifolia and the pretreatment of Betula Phellinus igniarius (L. ex Fr.) Quel. raw material；
(2) prepare extracting solution, and use supersound process, obtain sonification fluid；
(3) in sonification fluid prepared by described step (2), add compound enzyme, obtain enzymolysis solution；
(4) enzymolysis solution that described step (3) obtains is carried out microwave treatment, obtain microwave treatment liquid；
(5) use centrifuge to be centrifuged microwave treatment liquid, take the supernatant after being centrifuged；
(6) in supernatant, ethanol is added, and precipitate with ethanol 10-20h；
(7) alcohol deposit fluid in described step (6) is centrifuged, precipitation；
(8) precipitation is dissolved in a small amount of water, lyophilization, obtains polysaccharide component.
Further, as preferably, in described step (1), weigh urtica angustifolia and the Betula Phellinus igniarius (L. ex Fr.) Quel. of certain mass, use clear water
Cleaned, dried at 65 DEG C afterwards, then, use Chinese herbal medicine grinding and crushing machine to be ground, prepare material powder,
Utilize 50 mesh sieves that material powder is sieved for subsequent use；In described step (2), when preparing extracting solution, by the amount of solid-liquid ratio 1:40g/ml
In material powder, add deionized water, be uniformly mixing to obtain extracting solution, then, use the ultrasound wave of 600W power to extracting solution
Carry out supersound process 20min, and keep the temperature of extracting solution to be 40-45 DEG C.
Further, as preferably, in described step (3), compound enzyme at least includes cellulase, pectase and albumen
Enzyme, and the ratio of cellulase, pectase and protease is 1:1:1, after adding protease, the temperature keeping enzymolysis solution is 50
DEG C, PH is 4.5, and enzymolysis time is 150min.
Further, as preferably, in described step (4), during microwave treatment, the power of microwave is 650W, microwave treatment
Temperature is 55-60 DEG C, and microwave treatment time is 2-3min, and requires at interval of 5min, it to be carried out microwave treatment once, at least
Process 4 times.
Further, as preferably, in described step (5), when using centrifuge to be centrifuged, centrifugal rotating speed is
5000r/min。
Further, as preferably, in described step (6), the ethanol added in supernatant be mass fraction be the second of 92%
Alcohol, seals after addition, is placed in the water-bath of 5 DEG C, after insulation 20h, and sucking filtration precipitate with ethanol.
Further, as preferably, in described step (7), when being centrifuged alcohol deposit fluid, centrifugal rotational speed is 4000r/min.
Further, the invention provides the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel.
The application of the polysaccharide component prepared, it is characterised in that: it is used for preparing anti-oxidation medicine, anti-fatigue medicament, anti-tumor drug
Or hypertension drug.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention can reduce the extraction cost of polysaccharide component, improves extraction ratio, uses the extracting method of the present invention, extraction ratio energy
Higher than 75 m g/g, the method is easily controllable, simple to operate, and utilizes the method that ultrasonic assistant, microwave-assisted combine,
Substantially increasing extraction efficiency, safe, quick, energy-conservation, extraction time is short.
Accompanying drawing explanation
Fig. 1 is the flowage structure schematic diagram of the present invention.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.Based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under not making creative work premise
Embodiment, broadly falls into the scope of protection of the invention.
Referring to Fig. 1, the present invention provides a kind of technical scheme: polysaccharide in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel.
The preparation method of composition, it is characterised in that it comprises the following steps:
(1) urtica angustifolia and the pretreatment of Betula Phellinus igniarius (L. ex Fr.) Quel. raw material: weigh urtica angustifolia and the Betula Phellinus igniarius (L. ex Fr.) Quel. of certain mass, use clear water
Cleaned, dried at 65 DEG C afterwards, then, use Chinese herbal medicine grinding and crushing machine to be ground, prepare material powder,
Utilize 50 mesh sieves that material powder is sieved for subsequent use；
(2) prepare extracting solution, and use supersound process, obtain sonification fluid: when preparing extracting solution, by solid-liquid ratio 1:40g/ml
Amount in material powder, add deionized water, be uniformly mixing to obtain extracting solution, then, use 600W power ultrasound wave to carrying
Take liquid and carry out supersound process 20min, and keep the temperature of extracting solution to be 40-45 DEG C；
(3) in sonification fluid prepared by described step (2), add compound enzyme, obtain enzymolysis solution: compound enzyme at least includes
Cellulase, pectase and protease, and the ratio of cellulase, pectase and protease is 1:1:1, after adding protease,
The temperature keeping enzymolysis solution is 50 DEG C, and PH is 4.5, and enzymolysis time is 150min；
(4) enzymolysis solution that described step (3) obtains is carried out microwave treatment, obtain microwave treatment liquid: during microwave treatment, microwave
Power is 650W, and the temperature of microwave treatment is 55-60 DEG C, and microwave treatment time is 2-3min, and requires at interval of 5min it
Carry out microwave treatment once, at least process 4 times；
(5) use centrifuge to be centrifuged microwave treatment liquid, take the supernatant after being centrifuged: be centrifuged using centrifuge
Time, centrifugal rotating speed is 5000r/min；
(6) in supernatant, add ethanol, and precipitate with ethanol 10-20h: the ethanol added in supernatant be mass fraction be 92%
Ethanol, seals after addition, is placed in the water-bath of 5 DEG C, after insulation 20h, and sucking filtration precipitate with ethanol；
(7) alcohol deposit fluid in described step (6) is centrifuged, precipitation: when being centrifuged alcohol deposit fluid, centrifugal rotational speed is
4000r/min；
(8) precipitation is dissolved in a small amount of water, lyophilization, obtains polysaccharide component.
Present invention also offers the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel. to prepare
The application of polysaccharide component, it is characterised in that: its be used for preparing anti-oxidation medicine, anti-fatigue medicament, anti-tumor drug or
Hypertension drug.
The present invention can reduce the extraction cost of polysaccharide component, improves extraction ratio, uses the extracting method of the present invention, extracts
Rate can be higher than 75 m g/g, and the method is easily controllable, simple to operate, and utilizes ultrasonic assistant, microwave-assisted to combine
Method, substantially increases extraction efficiency, and safe, quick, energy-conservation, extraction time is short.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, permissible
Understand and these embodiments can be carried out multiple change without departing from the principles and spirit of the present invention, revise, replace
And modification, the scope of the present invention be defined by the appended.
Claims (8)
1. the preparation method of polysaccharide component in a Daxing'an Mountainrange urtica angustifolia and Betula Phellinus igniarius (L. ex Fr.) Quel., it is characterised in that it include with
Lower step:
(1) urtica angustifolia and the pretreatment of Betula Phellinus igniarius (L. ex Fr.) Quel. raw material；
(2) prepare extracting solution, and use supersound process, obtain sonification fluid；
(3) in sonification fluid prepared by described step (2), add compound enzyme, obtain enzymolysis solution；
(4) enzymolysis solution that described step (3) obtains is carried out microwave treatment, obtain microwave treatment liquid；
(5) use centrifuge to be centrifuged microwave treatment liquid, take the supernatant after being centrifuged；
(6) in supernatant, ethanol is added, and precipitate with ethanol 10-20h；
(7) alcohol deposit fluid in described step (6) is centrifuged, precipitation；
(8) precipitation is dissolved in a small amount of water, lyophilization, obtains polysaccharide component.
The preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia the most according to claim 1 and Betula Phellinus igniarius (L. ex Fr.) Quel.,
It is characterized in that: in described step (1), weigh urtica angustifolia and the Betula Phellinus igniarius (L. ex Fr.) Quel. of certain mass, cleaned with clear water, afterwards
Dry at 65 DEG C, then, use Chinese herbal medicine grinding and crushing machine to be ground, prepare material powder, utilize 50 mesh sieves pair
Material powder sieves for subsequent use；In described step (2), when preparing extracting solution, by the amount of solid-liquid ratio 1:40g/ml in material powder
Add deionized water, be uniformly mixing to obtain extracting solution, then, use the ultrasound wave of 600W power that extracting solution is carried out supersound process
20min, and keep the temperature of extracting solution to be 40-45 DEG C.
The preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia the most according to claim 1 and Betula Phellinus igniarius (L. ex Fr.) Quel.,
It is characterized in that: in described step (3), compound enzyme at least includes cellulase, pectase and protease, and cellulase,
The ratio of pectase and protease is 1:1:1, and after adding protease, the temperature keeping enzymolysis solution is 50 DEG C, and PH is 4.5, enzymolysis
Time is 150min.
The preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia the most according to claim 1 and Betula Phellinus igniarius (L. ex Fr.) Quel.,
It is characterized in that: in described step (4), during microwave treatment, the power of microwave is 650W, and the temperature of microwave treatment is 55-60 DEG C,
Microwave treatment time is 2-3min, and requires, at interval of 5min, it is carried out microwave treatment once, at least processes 4 times.
The preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia the most according to claim 1 and Betula Phellinus igniarius (L. ex Fr.) Quel.,
It is characterized in that: in described step (5), when using centrifuge to be centrifuged, centrifugal rotating speed is 5000r/min.
The preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia the most according to claim 1 and Betula Phellinus igniarius (L. ex Fr.) Quel.,
It is characterized in that: in described step (6), in supernatant add ethanol be mass fraction be the ethanol of 92%, carry out after addition
Seal, be placed in the water-bath of 5 DEG C, after insulation 20h, sucking filtration precipitate with ethanol.
The preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia the most according to claim 1 and Betula Phellinus igniarius (L. ex Fr.) Quel.,
It is characterized in that: in described step (7), when being centrifuged alcohol deposit fluid, centrifugal rotational speed is 4000r/min.
8. according to polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia described in claim 1-7 any one and Betula Phellinus igniarius (L. ex Fr.) Quel.
The application of the polysaccharide component that preparation method prepares, it is characterised in that: it is used for preparing anti-oxidation medicine, anti-fatigue medicament, resisting and swell
The medicine of tumor or hypertension drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610426470.3A CN106084081B (en) | 2016-06-16 | 2016-06-16 | The preparation method and products application of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610426470.3A CN106084081B (en) | 2016-06-16 | 2016-06-16 | The preparation method and products application of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106084081A true CN106084081A (en) | 2016-11-09 |
| CN106084081B CN106084081B (en) | 2018-09-21 |
Family
ID=57235940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610426470.3A Active CN106084081B (en) | 2016-06-16 | 2016-06-16 | The preparation method and products application of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106084081B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113265013A (en) * | 2021-06-10 | 2021-08-17 | 中国农业科学院草原研究所 | Method for extracting nettle polysaccharide by using ultrasonic-assisted complex enzyme |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060270626A1 (en) * | 2005-05-24 | 2006-11-30 | Hwang Hye J | Crude exopolysaccharides produced from phellinus baumii mycelium having hypoglycemic activity and preparation method thereof |
| CN101580552A (en) * | 2009-06-24 | 2009-11-18 | 张海悦 | Preparation method of urtica angustifolia polysaccharide and application thereof |
| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
-
2016
- 2016-06-16 CN CN201610426470.3A patent/CN106084081B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060270626A1 (en) * | 2005-05-24 | 2006-11-30 | Hwang Hye J | Crude exopolysaccharides produced from phellinus baumii mycelium having hypoglycemic activity and preparation method thereof |
| CN101580552A (en) * | 2009-06-24 | 2009-11-18 | 张海悦 | Preparation method of urtica angustifolia polysaccharide and application thereof |
| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
Non-Patent Citations (1)
| Title |
|---|
| 谢丽源等: "超声波复合酶法提取桑黄多糖研究", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113265013A (en) * | 2021-06-10 | 2021-08-17 | 中国农业科学院草原研究所 | Method for extracting nettle polysaccharide by using ultrasonic-assisted complex enzyme |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106084081B (en) | 2018-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104013652B (en) | Purification process, comprehensive utilization method and application of ganoderma lucidum spores powder | |
| CN102847134B (en) | Preparation method for chlorella polypeptide microcapsule | |
| US20140309414A1 (en) | Method for extracting polysaccharides from higher plants and fungi through microwave chemical treatment | |
| CN102030834B (en) | Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide | |
| CN102627789B (en) | Preparation process for edible fungus compound polysaccharide | |
| CN105504079A (en) | Process for producing astragalus polysaccharide by using ultrasonic technology | |
| CN104628881A (en) | Method for extracting auricularia polytricha polysaccharide | |
| CN113150179A (en) | Tremella aurantialba polysaccharide extraction and preparation technology | |
| CN104403019A (en) | Extraction method of compound edible fungi polysaccharide | |
| CN105400845A (en) | Technology for producing radix astragali polysaccharide by utilization of microbial fermentation technology | |
| Yan et al. | New polysaccharide compounds derived from submerged culture of Ganoderma lucidum and Lycium barbarum | |
| CN106084081B (en) | The preparation method and products application of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus | |
| CN111269954B (en) | Method for preparing ganoderma lucidum spore powder exine crude polysaccharide by using enzyme method assisted ultrasound | |
| CN102453105A (en) | Extraction method for polysaccharide | |
| CN104068357A (en) | Preparation method of chili dietary fibers | |
| CN105504085B (en) | Cordyceps militaris glucan and its preparation method and application | |
| CN101899120A (en) | Method for refining jujube polysaccharide | |
| CN101619108B (en) | Method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves | |
| CN108619243B (en) | Method for synergistically extracting active ingredients in ramulus mori (leaves) by solid acid enhanced hydrothermal reaction | |
| CN103550147A (en) | Ganoderma lucidum polysaccharide injection and preparation method thereof | |
| CN106728208A (en) | A kind of ginseng and lucid ganoderma polysaccharide triterpene preparation and preparation method thereof | |
| CN113105567B (en) | Paecilomyces cicadae mannan and preparation and application thereof | |
| CN104739916A (en) | Alpha-glucosidase active inhibitor as well as preparation method and application thereof | |
| CN107857822A (en) | The method that polysaccharide is extracted from blood clam | |
| CN106754057A (en) | A kind of health care red starter glutinous wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |