CN106074404A - A kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products and preparation method thereof - Google Patents
A kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products and preparation method thereof Download PDFInfo
- Publication number
- CN106074404A CN106074404A CN201610678206.9A CN201610678206A CN106074404A CN 106074404 A CN106074404 A CN 106074404A CN 201610678206 A CN201610678206 A CN 201610678206A CN 106074404 A CN106074404 A CN 106074404A
- Authority
- CN
- China
- Prior art keywords
- liposome
- respiratory syndrome
- porcine reproductive
- dried products
- syndrome virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/28—Steroids, e.g. cholesterol, bile acids or glycyrrhetinic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/10011—Arteriviridae
- C12N2770/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Abstract
A kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products and preparation method thereof, belongs to veterinary biologics technical field.Containing following components in every 1000mlPBS solution: Radix Astragali saponin III 2 8g, Aloe polysaccharide 5 10g, curcumin 2 6g, liposome 10 15g, glucose 10 15g and mannitol 10 15g.Liposome dilution freeze-dried products prepared by the present invention can reduce the differences between batches of semi-finished product.Liposome dilution freeze-dried products prepared by the present invention can promote the breeding of porcine reproductive and respiratory syndrome virus, improves viral level, strengthens the immunogenicity of vaccine.
Description
Technical field
The invention belongs to veterinary biologics technical field, be specifically related to a kind of porcine reproductive and respiratory syndrome virus lipid
Body diluent freeze-dried products and preparation method thereof.
Background technology
Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome,
PRRS), it is a kind of high degree in contact sexually transmitted disease being caused pig by porcine reproductive and respiratory syndrome virus (PRRSV), not the same year
The pig of age, kind and sex all can infect, and this disease is with sow breeding difficulty, piglet respiratory symptom, immunosuppressant, high infection
The highly pathogenic epidemic disease of one that rate, high mortality are characterized.This disease causes serious economic loss to whole world pig industry, in order to
Efficiently control this disease, the most develop the multiple vaccine for preventing PRRS.
Liposome is that one has a same biomembrane kin phospholipid bilayer structure carrier, and Chinese medicine liposome system
The spherical drug carriers formulations of a kind of superminiature referring to that middle the effective elements of the medicine is encapsulated in lipoids bilayer and make.This medicine
Thing carrier has the advantage of targeting, sustained-release administration, and liposome to normal cell and organizes harmless and inhibitory action, also may be used
To show good cellular affinity and histocompatibility.Liposome is the most degradable, avirulence and non-immunogenicity, its
It is possible not only to improve drug therapeutic indices as a kind of pharmaceutical carrier, additionally it is possible to reduce drug toxicity and reduce poisonous side effect of medicine
Etc. advantage.
The Radix Astragali is the most important Chinese medicine, has benefit and defends the effects such as consolidating superficial resistance, diuretic be swollen clearly, promoting pus discharge and tissue regeneration strengthening, invigorating the spleen and replenishing QI, the people
Between be usually used in treating spontaneous perspiration, night sweat, arthralgia due to stagnation of blood, edema, cellulitis do not burst or burst do not hold back for a long time, interior part band void, diarrhea due to hypofunction of the spleen and all gas
The disease of the blood deficiency that declines.Radix Astragali saponin is one of main active of the Radix Astragali, and Radix Astragali saponin is possible not only to promote lymphocytic hyperplasia and resist
The increase of body, moreover it is possible to promote humoral and cellular immune response responsing reaction.
Polysaccharide is one of active ingredient of Chinese herbs, and numerous studies show, polysaccharide and saccharide complex are not only work in vivo
For energy resource and constituent material, it is often more important that it is present in all membrane structures, participate in cell in biosis
Various activities.Polysaccharose substance is the important component part of all Living organisms, has removing free radical, improves antioxidation
Enzymatic activity and the ability of anti-lipid peroxidation
One of most important index of veterinary biologics performance rating is exactly the stability between batch, but in actual production process
In be difficult to control to stability between the batch of semi-finished product, mainly ratio when inoculating living body biological of tracing it to its cause is uncertain, also
That is inoculum concentration instability causes producing semi-finished product bigger difference.Present inventive concept is to solve pig breeding and breathe
The difficult problem that in syndrome virus breeding, between batch, difference is big and viral level is too low.
Summary of the invention
The problem existed for prior art, it is an object of the invention to design provides a kind of Porcine reproductive and respiratory syndrome
The technical scheme of viral lipid body diluent freeze-dried products and preparation method thereof.
Described a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products, it is characterised in that every
Containing following components in 1000mlPBS solution: Radix Astragali saponin III 2-8g, Aloe polysaccharide 5-10g, curcumin 2-6g, liposome
10-15g, glucose 10-15g and mannitol 10-15g.
Described a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products, it is characterised in that every
Containing following components in 1000mlPBS solution: Radix Astragali saponin III 4-6g, Aloe polysaccharide 6-8g, bisdemethoxycurcumin-5g, liposome 12-
14g, glucose 12-14g and mannitol 12-14g.
Described a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products, it is characterised in that described
Liposome is prepared by following steps: weighs soybean lecithin, cholesterol, dissolves with chloroform, and makes its mix homogeneously, and
Remove Chlorine in Solution by decompression method to imitate, prepare liposome membrane solution；Take the acid of Chinese holly edge and Chinese holly edge acid potassium that pH value is 7.0～8.0
Buffer solution, joins this buffer solution in liposome membrane solution, is prepared as liposome with refiner after dehydration.
Described a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products, it is characterised in that described
PBS solution is prepared by following steps: take sodium chloride 6-10g, potassium chloride 0.05-0.5g, disodium hydrogen phosphate 1-1.2g, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.05-0.5g, calcium chloride 0.05-0.2g, magnesium chloride 0.05-0.2g containing 6 water of crystallization；Above each composition is mixed
After, it is dissolved in 1000ml distilled water, through 116 DEG C, autoclaving 30 minutes and get final product.
The preparation method of described a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products, its feature
It is to comprise the following steps: take after the component of described weight is sufficiently mixed, join water in the PBS solution of 1000ml pH 7
Change, then filter through the filter of 0.22um, be sub-packed in lyophilizing bottle, first pre-freeze 4-8h at-40 DEG C, then put vacuum freeze-drying
Machine is dried, i.e. obtains porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products.
The preparation method of described a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products, its feature
It is that described PBS solution is prepared by following steps: take sodium chloride 6-10g, potassium chloride 0.05-0.5g, disodium hydrogen phosphate 1-
1.2g, potassium dihydrogen phosphate 0.05-0.5g, calcium chloride 0.05-0.2g, magnesium chloride 0.05-0.2g containing 6 water of crystallization；More than Jiang
After the mixing of each composition, it is dissolved in 1000ml distilled water, through 116 DEG C, autoclaving 30 minutes and get final product.
In the present invention, Radix Astragali saponin III is by Chengdu Man Site bio tech ltd production and sales, and Aloe polysaccharide is by Lanzhou
Water Lai Si bio tech ltd's production and sales, curcumin is by the production and sales of lark prestige Science and Technology Ltd..
The method have the advantages that
1, the liposome dilution freeze-dried products prepared by the present invention can reduce the differences between batches of semi-finished product
2, the liposome dilution freeze-dried products prepared by the present invention can promote the breeding of porcine reproductive and respiratory syndrome virus,
Improve viral level, strengthen the immunogenicity of vaccine.
Detailed description of the invention
In order to make the present invention easier to understand, below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that
These embodiments are merely to illustrate the present invention rather than limit the scope of the present invention, NM concrete reality in the following example
Proved recipe method, experimental technique is carried out the most routinely.
The preparation of embodiment 1:PBS solution
Take sodium chloride 6-10g, potassium chloride 0.05-0.5g, disodium hydrogen phosphate 1-1.2g, potassium dihydrogen phosphate 0.05-0.5g, calcium chloride
0.05-0.2g, magnesium chloride 0.05-0.2g containing 6 water of crystallization；
After above each composition is mixed, it is dissolved in 1000ml distilled water, through 116 DEG C, autoclaving 30 minutes and get final product.
Embodiment 2: the preparation of liposome
Weigh soybean lecithin 6g, cholesterol 2g, dissolve with 500ml chloroform, and make its mix homogeneously, and by decompression method
Removal Chlorine in Solution is imitated, and prepares liposome membrane solution；Take the acid of Chinese holly edge and Chinese holly edge acid potassium buffer solution that pH value is 7.0～8.0
200ml, joins this buffer solution in liposome membrane solution, is prepared as liposome with refiner after dehydration.
Embodiment 3: the preparation of liposome
Weigh soybean lecithin 4g, cholesterol 4g, dissolve with 500ml chloroform, and make its mix homogeneously, and by decompression method
Removal Chlorine in Solution is imitated, and prepares liposome membrane solution；Take the acid of Chinese holly edge and Chinese holly edge acid potassium buffer solution that pH value is 7.0～8.0
200ml, joins this buffer solution in liposome membrane solution, is prepared as liposome with refiner after dehydration.
Embodiment 4: the preparation of a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products
Take Radix Astragali saponin III 2g, Aloe polysaccharide 5g, curcumin 2g, liposome (embodiment 2 prepares) 10g, glucose 10g, manna
Alcohol 10g；
Take after the component of described weight is sufficiently mixed, join water in the PBS solution (embodiment 1 prepares) of 1000ml pH 7
Change, then filter through the filter of 0.22um, be sub-packed in lyophilizing bottle, first pre-freeze 6h at-40 DEG C, then put vacuum freeze-drying machine
In be dried, i.e. obtain porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products.
Embodiment 5: the preparation of a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products
Take Radix Astragali saponin III 4g, Aloe polysaccharide 6g, bisdemethoxycurcumin g, liposome (embodiment 3 prepares) 12g, glucose 13g, manna
Alcohol 12g；
Take after the component of described weight is sufficiently mixed, join water in the PBS solution (embodiment 1 prepares) of 1000ml pH 7
Change, then filter through the filter of 0.22um, be sub-packed in lyophilizing bottle, first pre-freeze 4h at-40 DEG C, then put vacuum freeze-drying machine
In be dried, i.e. obtain porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products.
Embodiment 6: the preparation of a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products
Take Radix Astragali saponin III 8g, Aloe polysaccharide 10g, curcumin 6g, liposome (embodiment 3 prepares) 15g, glucose 15g, manna
Alcohol 15g；
Take after the component of described weight is sufficiently mixed, join water in the PBS solution (embodiment 1 prepares) of 1000ml pH 7
Change, then filter through the filter of 0.22um, be sub-packed in lyophilizing bottle, first pre-freeze 8h at-40 DEG C, then put vacuum freeze-drying machine
In be dried, i.e. obtain porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products.
The application of test example 1 present invention
1 material
1.1 porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products
Prepare by embodiment of the present invention 4-6
1.2 porcine reproductive and respiratory syndrome virus (CH-1a strain), Marc-145 cell
1.3 25 age in days health ablactational baby pig, ELISA detection PRRS antibody is negative detection pseudorabies antibody, antigen pair
Negative
2 methods
The mensuration of 2.1 envelop rates
The freeze-dried lipidosome taking blank respectively and the lipidosome freeze-dried powder processed, redissolve in phosphate buffer, super
Speed is centrifugal, takes after supernatant dilutes by a certain percentage, measures its absorbance (with reference to veterinary drug allusion quotation), and average each sample is surveyed 3 times, calculates it
Meansigma methods.
2.2 morphologic observations and particle diameter
Liposome dilution lyophilized powder, after PBS redissolves, is observed under transmission electron microscope (× 20000)
2.3 cells are cultivated and connect poison
Cell culture processes cultivates Marc-145 cell routinely；4 groups are divided equally, often group 3 bottles after passage, the present invention is real
Execute liposome dilution freeze-dried products prepared by example 4-6 to be placed in PH7 phosphate buffer and redissolve, respectively will
20160701,20160702, the viral dilution of 20,160,703 3 kind of different batches becomes 1000 TCID50/ml、1500 TCID50/
ml、2000 TCID50/ml.A group, the liposome dilution of the employing embodiment of the present invention 4 preparation PRRSV to 3 kinds of different batches
It is diluted inoculation；B group, uses the liposome dilution of the embodiment of the present invention 5 preparation to carry out dilute to 3 kinds of different batches PRRSV
Release inoculation；C group, uses the liposome dilution of the embodiment of the present invention 6 preparation that 3 different batches PRRSV are diluted inoculation；D
Group, matched group, use the cell culture fluid of serum-free that 3 different batches PRRSV are diluted inoculation, above each group is put respectively
In 37 DEG C, 5% CO2 cell culture incubator cultivates 72 h after carry out receiving poison.
2.4 TCID50Measure
Postdigestive cell will be passed on and be diluted to 105～106After individual/m L, proceed in 96 well culture plates, by 0. 2 m L
/ hole；Four papova liquid of results are made continuous print 10 times dilution respectively, by 0.2 mL/hole inoculation, is placed in 37 DEG C absorption
After 2h, adding in the DMEM cell maintenance medium containing 2% serum, be placed in 37 DEG C, cultivate in the incubator of 5%, every day, observation of cell was sick
Become and record, calculate TCID by Reed-Muench method50。
2.5 immunogenicity determining
The cultivation 72h 20160701 batch three papova liquid mentioned of results 2.3 are qualified through inspection, carry out respectively inactivateing, emulsifying
After be fabricated to vaccine finished product for measuring the immunogenicity of vaccine.Ablactational baby pig being divided into 4 groups, often group 6, the Ith group uses warp
The vaccine made after the PRRSV inactivation emulsifying that the liposome dilution of the embodiment of the present invention 4 preparation processed carries out immunity；IIth
The vaccine immunity that group is made after using the PRRSV inactivation emulsifying that the liposome dilution prepared through the embodiment of the present invention 5 processed；
IIIth group is the epidemic disease made after the PRRSV using the liposome dilution prepared through the embodiment of the present invention 6 to process inactivates emulsifying
Seedling immunity；IVth group, vaccine matched group, the vaccine immunity made after inactivateing without liposome-treated；Successively immunity 2 times, first
Secondary immunity 2ml/ head；After the 20d of interval, carry out the 2nd immunity, carry out immunity by Isodose.Each group all head exempt from the 14th d,
21d, 28d, 35 d, 45d, 60d, 90d, 120d, 180d take a blood sample and separate serum, are measured by ELISA method.
3 results
3.1 envelop rate results
According to formula: envelop rate (%)=system is encapsulated and non-encapsulated total dose (A2) in dose (the A1)/system of encapsulating
× 100%, the results are shown in Table 1.
Table 1 entrapment efficiency determination result
3.2 morphologic observations and particle size determination
Liposome dilution freeze-dried products is multilamelar liposome, and granularity rule, for circle, particle diameter is basically identical, the results are shown in Table 2.
Table 2 morphologic observation and particle size determination result
3.3 TCID50Measurement result
Table 3 TCID50Measurement result
3.4 Serum Antibody Detection results
Table 4 TPPA result
4 conclusions
In sum, porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products of the present invention has good encapsulating
Rate, and granularity rule, particle diameter is basically identical；Can be good at ensureing the stability of semi-finished product inoculum concentration, reduce pig breeding and exhale
Inhale the batch wise differences between syndrome virus inactivated vaccine semi-finished product；Improve the serum antibody titer of vaccine, strengthen the immunity of vaccine
Originality.
Claims (6)
1. a porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products, it is characterised in that every 1000mlPBS is molten
Containing following components in liquid: Radix Astragali saponin III 2-8g, Aloe polysaccharide 5-10g, curcumin 2-6g, liposome 10-15g, glucose
10-15g and mannitol 10-15g.
2. a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products as claimed in claim 1, its feature
It is in every 1000mlPBS solution containing following components: Radix Astragali saponin III 4-6g, Aloe polysaccharide 6-8g, bisdemethoxycurcumin-5g, fat
Plastid 12-14g, glucose 12-14g and mannitol 12-14g.
3. a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products as claimed in claim 1 or 2, it is special
Levy and be that described liposome is prepared by following steps: weigh soybean lecithin, cholesterol, dissolve with chloroform, and make
Its mix homogeneously, and it is imitative to remove Chlorine in Solution by decompression method, prepares liposome membrane solution；Take the Chinese holly edge that pH value is 7.0～8.0
Acid and Chinese holly edge acid potassium buffer solution, join this buffer solution in liposome membrane solution, be prepared as fat with refiner after dehydration
Plastid.
4. a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products as claimed in claim 1 or 2, it is special
Levy and be that described PBS solution is prepared by following steps: take sodium chloride 6-10g, potassium chloride 0.05-0.5g, disodium hydrogen phosphate
1-1.2g, potassium dihydrogen phosphate 0.05-0.5g, calcium chloride 0.05-0.2g, magnesium chloride 0.05-0.2g containing 6 water of crystallization；Will be with
After upper each composition mixing, it is dissolved in 1000ml distilled water, through 116 DEG C, autoclaving 30 minutes and get final product.
The system of a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products the most as claimed in claim 1 or 2
Preparation Method, it is characterised in that comprise the following steps: take after the component of described weight is sufficiently mixed, join 1000ml pH's 7
Aquation in PBS solution, then filter through the filter of 0.22um, it is sub-packed in lyophilizing bottle, first pre-freeze 4-8h at-40 DEG C,
Put again in vacuum freeze-drying machine and be dried, i.e. obtain porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products.
The preparation side of a kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products the most as claimed in claim 5
Method, it is characterised in that described PBS solution is prepared by following steps: take sodium chloride 6-10g, potassium chloride 0.05-0.5g, phosphoric acid
Disodium hydrogen 1-1.2g, potassium dihydrogen phosphate 0.05-0.5g, calcium chloride 0.05-0.2g, magnesium chloride 0.05-containing 6 water of crystallization
0.2g；After above each composition is mixed, it is dissolved in 1000ml distilled water, through 116 DEG C, autoclaving 30 minutes and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610678206.9A CN106074404A (en) | 2016-08-17 | 2016-08-17 | A kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610678206.9A CN106074404A (en) | 2016-08-17 | 2016-08-17 | A kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106074404A true CN106074404A (en) | 2016-11-09 |
Family
ID=58069566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610678206.9A Pending CN106074404A (en) | 2016-08-17 | 2016-08-17 | A kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106074404A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106821966A (en) * | 2016-11-29 | 2017-06-13 | 浙江美保龙生物技术有限公司 | A kind of live vaccines of hog cholera dilution |
| CN106983765A (en) * | 2017-05-15 | 2017-07-28 | 江苏省农业科学院 | A kind of traditional Chinese medicine ingredients prescription for preventing and treating Porcine circovirus desease and its preparation method and application |
| CN107267467A (en) * | 2017-07-31 | 2017-10-20 | 浙江美保龙生物技术有限公司 | A kind of separation method of porcine reproductive and respiratory syndrome virus |
| CN115737573A (en) * | 2022-11-30 | 2023-03-07 | 杭州沐源生物医药科技有限公司 | Latamoxef sodium freeze-dried powder for injection and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004002453A1 (en) * | 2002-06-28 | 2004-01-08 | Protiva Biotherapeutics Ltd. | Method and apparatus for producing liposomes |
| CN105505887A (en) * | 2015-12-29 | 2016-04-20 | 浙江美保龙生物技术有限公司 | Porcine reproductive and respiratory syndrome virus diluent and preparation method thereof |
-
2016
- 2016-08-17 CN CN201610678206.9A patent/CN106074404A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004002453A1 (en) * | 2002-06-28 | 2004-01-08 | Protiva Biotherapeutics Ltd. | Method and apparatus for producing liposomes |
| CN105505887A (en) * | 2015-12-29 | 2016-04-20 | 浙江美保龙生物技术有限公司 | Porcine reproductive and respiratory syndrome virus diluent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张天赐，等: "《张天赐 神经外科学》", 31 January 2014 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106821966A (en) * | 2016-11-29 | 2017-06-13 | 浙江美保龙生物技术有限公司 | A kind of live vaccines of hog cholera dilution |
| CN106983765A (en) * | 2017-05-15 | 2017-07-28 | 江苏省农业科学院 | A kind of traditional Chinese medicine ingredients prescription for preventing and treating Porcine circovirus desease and its preparation method and application |
| CN107267467A (en) * | 2017-07-31 | 2017-10-20 | 浙江美保龙生物技术有限公司 | A kind of separation method of porcine reproductive and respiratory syndrome virus |
| CN115737573A (en) * | 2022-11-30 | 2023-03-07 | 杭州沐源生物医药科技有限公司 | Latamoxef sodium freeze-dried powder for injection and preparation method thereof |
| CN115737573B (en) * | 2022-11-30 | 2024-02-20 | 杭州沐源生物医药科技有限公司 | Cephalosporium oxydanum freeze-dried powder for injection and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106074404A (en) | A kind of porcine reproductive and respiratory syndrome virus liposome dilution freeze-dried products and preparation method thereof | |
| Gu et al. | Optimization of angelica sinensis polysaccharide-loaded Poly (lactic-co-glycolicacid) nanoparticles by RSM and its immunological activity in vitro | |
| CN104739888A (en) | Eurotium cristatum powder as well as preparation method and application thereof | |
| CN103800661B (en) | A kind of enhancing immunity, alleviating physical fatigue compositions and containing its preparation | |
| CN104873624B (en) | A kind of pharmaceutical composition for gouty arthritis | |
| CN110368402A (en) | Mescenchymal stem cell preparation and its preparation method and application | |
| CN109999099A (en) | A kind of Chinese medicine composition and its preparation method and application for the deficiency of blood | |
| CN106109424A (en) | A kind of porcine circovirus 2 type liposome dilution freeze-dried products and preparation method thereof | |
| CN103417518B (en) | Oxidized resveratrol is as the application of preparation tumor | |
| CN109395099B (en) | Method for improving stability of tuberculin BCG-PPD skin test diagnostic reagent | |
| TW201221133A (en) | A medical compositions for inhibition of influenza Hemagglutinin and process for the preparation thereof | |
| CN103127055A (en) | Application of tangeretin serving as sensitizer used for preparing chemotherapeutics used for curing solid tumor | |
| CN106074402A (en) | A kind of pig parvoviral liposome dilution freeze-dried products and preparation method thereof | |
| CN106176634A (en) | A kind of transmissible gastroenteritis of swine epidemic diarrhea Bi-combined attenuated Vaccine liposome dilution freeze-dried products and preparation method thereof | |
| WO2016141774A1 (en) | Uses of jilin ginseng oligopeptide in preparing food product or healthcare food product for improving and enhancing sexual function | |
| CN106137982A (en) | A kind of pseudorabies living vaccines liposome dilution freeze-dried products and preparation method thereof | |
| CN105456185A (en) | Compound Astragalus polysaccharides injection and preparation method thereof | |
| CN106562934A (en) | Preparation method of porcine epidemic diarrhea virus liposome diluent freeze-dried product | |
| CN107753823A (en) | A kind of Chinese medicine composition for treating or preventing hand-foot-and-mouth disease | |
| CN103285391A (en) | Combined vaccine of seasonal influenza and pandemic influenza for people and preparation method | |
| CN104208664B (en) | A kind of nose vaccine combination liquid preparation and preparation method thereof | |
| CN108289948A (en) | The manufacturing method of oral administration pharmaceutical vaccine compositions and oral administration pharmaceutical vaccine compositions | |
| CN110327317A (en) | Application of the alkannin in the drug for preparing anti-rotavirus infection | |
| CN105030777A (en) | Compound capable of enhancing curative effect of ALK-TKI and delaying drug resistance of same and preparation of compound | |
| CN103599337A (en) | Garlic saponin liposome and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161109 |