A kind of cosmetics with moisturizing whitening acne-removal function
Technical field
The present invention relates to a kind of cosmetics with moisturizing whitening acne-removal function, for the moisturizing whitening anti-acne merit of skin
Energy.
Background technology
Skin plays the effect of important barrier in human body, and it can protect human body not encroached on by extraneous factor.This screen
Barrier function is the function of a kind of protectiveness, it can protect human body not by various external irritants (as chemicals, atmosphere pollution,
The environment being dried and ultraviolet), and can prevent the moisture of excess from passing through skin from internal loss.When the cutin containing keratinocyte
When layer is maintained at normal condition, this protecting function can be maintained.
It is positioned at the outermost horny layer of epidermis (cuticular layer) to be formed by keratinocyte, and containing the keratinocyte broken up and ring
It is wound on the lipid layer around these keratinocytes.Keratinocyte is the typical cells produced by such a processes result: this mistake
The basal cell constantly bred at the basal layer of epidermis in journey is pushed upwardly to skin surface, and its shape and function are the most gradually sent out simultaneously
Give birth to change.Through the regular hour, old keratinocyte comes off from skin surface, and newborn keratinocyte replaces the cell come off
Play a role.This repetition and continuous print process are referred to as differentiation or the keratinization of epidermis cell.It addition, keratinocyte is at cutin
Shape while nature moisturizing factor (NMFs) and lipid within endothelial cells (neural phthalein amine, cholesterol, fatty acid etc.) is produced during change
Having become horny layer, this makes horny layer have robustness and pliability, finally makes it play the effect of skin barrier.But horny layer is non-
Chang Rongyi causes afunction because of various factors, such as, live and custom factor, and such as mistake washes one's face frequently and has a bath, environment
Factor, such as dry climate, pollutant etc. and endogenous disease, such as atopic dermatitis and senile skin convulsion are itched disease.Along with
The substantial increase of current various harmful factor, the dry skin patient's condition and associated disease are also stepped up by many.
At present, a lot of scientific research personnel have been carried out many and pay close attention to and carry out moisturizing from external source or prevent moisture from people
Body loss is to keep the moisture research at proper level of skin, and oneself is through developing the multiple guarantor with moisture holding capacity
Humectant.Generally by lipid components (such as neural phthalein amine) and in horny layer can be improved the material of water-retaining property (such as essential fatty acid and fat
Matter complex) it is used as wetting agent.But at present the factor that skin is harmful is constantly being increased, cuticular generation and exfoliation rates
Because of the change of dietary habit just slack-off, the amount of Moisture factor and lipid also due to keratinocyte functional deterioration and be reduced.
Along with expanding economy and growth in the living standard, people increasingly pay attention to the natural whitening of skin, and anti-acne is existing
Row women improves looks current greatest problem, and pox is being commonly called as of acne, also known as " comedo ", " skin sore " or " acne ", is to betide
Pilosebaceous a kind of chronic inflammation disease, be mainly manifested in clinically face, chest and back occur acne, pimple,
Pustule, cyst and tuberosity, the formed cicatrix having, have a strong impact on the appearance of people, employment, social activity and the marriage and childbirth etc. to normal person
Produce material impact.It is now recognized that the morbidity of acne and androgen levels, sebum secretion increase, hair follicle conduit hyperkeratosis, Cuo
Skin ulcer propionibacterium infects relevant.
Along with the raising of people's living standard, rhythm of life is accelerated, and life stress strengthens to be affected with environmental pollution etc., acne
Sickness rate is in rising trend, and age of onset is also widened, and the course of disease extends, and mostly is Persistent Acne of Different, has a strong impact on the body of people
Heart health and quality of life.Propionibacterium acnes is gram-negative facultatively anacrobic rod, and main field planting is at human skin pilosebaceous unit
Folliculus, for opportunistic pathogenic bacterium, it adapts to various complicated, and makes its cell wall from neutrophilic granulocyte and macrophage
Degraded, moreover it is possible to suppression t cell activation.Its special physio-biochemical characteristics determine the mechanism of causing a disease of its complexity, and and then cause
The various clinical disease such as acne.There are some researches show, propionibacterium acnes stimulate keratinocyte produce proinflammatory cell because of
Son such as IL-la, IL_10, IL-8, TNF_a, these cytokines can promote again other release of cytokines, therefore, acne propanoic acid
Bacillus can be set up a positive feedback loop by these cytokines and be maintained, promotes inflammatory process.
At present, the cosmetics on market are of a great variety, such as cosmetic cream, beauty cream, fair complexion cream, cleansing milk etc., its formula
Typically all contain octadecanol, glycerol, ethyl hydroxybenzoate, silicone oil, monoglyceride, water etc., the production technology of this kind of cosmetics and
Formula is essentially all similar, the effect of beauty treatment protected to human body skin the most in various degree, but effect is not fine,
Facial acne, comedo are not the most had the effect of nursing for treating.Therefore, find efficient, lasting and human body is had no side effect
Natural whiting anti-acne preparations, it has also become the at present trend of the most pharmaceutically and cosmetically area research.
Although China's Chinese herbal medicine resource is abundant, natural plants pharmacy is with a long history, but relevant scientific development and application
Research relatively lags behind.Have some compositions separated from Chinese herbal medicine at present to be widely used in whitening product, such as Folium Vaccinii vitis-idaeae
Glycosides and Radix Glycyrrhizae extract etc., have become as the most the most frequently used whitening composition through repeatedly studying with on probation.Also just because of seeing
The conventional medicament unlimited potentiality in whitening field, increasing people will find the object set of efficient, lasting whitening composition
In on natural drug.Natural plant whitening composition sensitization rate is low, safety is high, has broad application prospects.
Summary of the invention
It is an object of the invention to provide a kind of whitening acne-eliminating cosmetic, the active component of this cosmetic is by following weight parts proportioning
Natural herbs raw material prepare: Radix Scutellariae: 3-50, Flos Chrysanthemi Indici 3~50, Flos Lonicerae 3~50, Fructus Gardeniae Cortex MoutanRadix Paeoniae Rubra: 3-50.
The present invention proposes kind of a whitening acne-eliminating cosmetic, it is characterised in that also added one in above-mentioned active component
Plant active polypeptide.Described active polypeptide can significantly improve anti-acne and whitening, the effect of lock water.
The sequence of described active polypeptide is that SEQ ID NO:1-25 is arbitrary shown.It is respectively designated as MGMB-1~25.
The present invention additionally provides the preparation method of a peptide species, Fructus Chaenomelis is cleaned up by (1), rubs, squeezes the juice, and adds silk ammonia
Pepsin and trypsin, enzyme concentration 20000IU/g Fructus Chaenomelis, hydrolysis temperature 45 DEG C, pH value 7.0, enzymolysis time 3h, enzymolysis is complete
Become rear 95 DEG C of enzyme denaturing 10min;
(2) material filtering after enzyme denaturing removes insoluble matter, obtains solution, and gained polypeptide solution adds 4% activated carbon adsorption
Decolouring, carries out peptide separation, 20mmol/L HCl solution eluting, flow velocity 1.3mL/ with glucosan G-50 (Sephadex G-50)
Minute, collect the eluted product of different time sections respectively, regulation solution is to pH7.0, and 10000 revs/min are centrifuged 15 minutes, through big
After the resin DA201-C desalting processing of hole, being concentrated in vacuo, supernatant lyophilization is standby;The separator of different time sections is through merit
Being able to verify that, the sequence that there are 25 little peptides has whitening, the function of lock water promotion anti-acne effect.Can obtain corresponding in batches
Little peptide, it is possible to the polypeptide described in synthetic.The sequence of described polypeptide is as shown in SEQ ID NO:1-25.Also can synthetic institute
The polypeptide stated.
Cosmetics of the present invention can be used as preparing sunscreen cream, sunlight lotion, solarization after product, day cream, late frost, facial film, skin cream, wash
Face milk, skin smoothing powder, eye, send out film, hair conditioner, quintessence oil and in color make-up product, such as deodorant stick, perfume bar, curry etc., above-mentioned
Product by the technology manufacture known to the industry.It is particularly useful for making cream, Emulsion, gel, facial film unguentum.
The invention has the beneficial effects as follows:
The cosmetics of the offer of the present invention can significantly whitening, anti-acne increase skin lock water function.The present invention uses grass
This medicine and the cosmetics that polypeptide is that raw material prepares, natural, gentle, non-stimulated to skin, and various active material association
Same-action is in skin, and its effect is better than ordinary cosmetics.
The detailed description of preferred embodiment
Following example illustrate each aspect of the present invention content.They, only in order to illustrate, do not limit the model of the present invention
Enclose.
The preparation of embodiment 1 polypeptide
(1) Fructus Chaenomelis is cleaned up, rub, squeeze the juice, add serine protease and trypsin, enzyme concentration 20000IU/
G Fructus Chaenomelis, hydrolysis temperature 45 DEG C, pH value 7.0, enzymolysis time 3h, enzymolysis complete after 95 DEG C of enzyme denaturing 10min;
(2) material filtering after enzyme denaturing removes insoluble matter, obtains solution, and gained polypeptide solution adds 4% activated carbon adsorption
Decolouring, carries out peptide separation, 20mmol/L HCl solution eluting, flow velocity 1.3mL/ with glucosan G-50 (Sephadex G-50)
Minute, collect the eluted product of different time sections respectively, regulation solution is to pH7.0, and 10000 revs/min are centrifuged 15 minutes, through big
After the resin DA201-C desalting processing of hole, being concentrated in vacuo, supernatant lyophilization is standby;The polypeptide warp of the collection of different time sections
Crossing functional verification, the sequence that there are 25 little peptides has whitening, the function of lock water promotion anti-acne effect.Phase can be obtained in batches
The little peptide answered, it is possible to the polypeptide described in synthetic.
The preparation of embodiment 2 cosmetics
Take Radix Scutellariae 500g, Flos Chrysanthemi Indici 300g, Flos Lonicerae 500g, Fructus Gardeniae 300g, Cortex Moutan 500g, Radix Paeoniae Rubra 400g.By above-mentioned
Raw material follows these steps to be prepared as blemish clearing gel: Radix Scutellariae, Flos Chrysanthemi Indici, Flos Lonicerae, Fructus Gardeniae, Cortex Moutan, Radix Paeoniae Rubra are extracted through decocting in water
After concentration, adding ethanol precipitation, stand, filter, filtrate recycling ethanol, to without alcohol taste, obtains Chinese herbal medicine concentrated solution.By on concentrated solution
Macroporous resin column, washing, ethanol elution, collect eluent, eluent reclaims ethanol, concentrates, and is dried, obtains chocolate brown powder group
Close extract 100g.
The preparation method of above-mentioned pox-eliminating whitening lock hydrogel product, comprises the following steps:
(1) being mixed by butanediol 5ml and MGMB-1 polypeptide 1g, add deionized water 5ml, stirring makes it dissolve;
(2) acrylic acid dimethyltaurine ammonium/VP copolymer 1 .5g is added, stirring while adding, i.e. obtain after stirring
Bright gel-type vehicle;
(3) taking combined extracts 10g, add propylene glycol 7ml, stirring makes it dissolve, makes solution;
(4) by combined extracts solution and gel-type vehicle mixing and stirring;
(5) regulation pH value is extremelyBetween;
(6) above-mentioned gel is tested, qualified after, then carry out fill and i.e. obtain blemish clearing gel skin care item.
The preparation of embodiment 3 cosmetics MGMB-2~25
According to the method for embodiment 2, polypeptide is replaced with respectively MGMB-2~25 totally 24 kinds of forms, remaining component and preparation
Method is identical with embodiment 1, prepares described cosmetics.
Embodiment 4 compares the preparation of cosmetics
According to the method for embodiment 2, being wherein added without corresponding polypeptide, remaining component and method are same as in Example 2, system
For obtaining corresponding cosmetics.
Embodiment 5 security verification
Test material and method
Animal: regular grade laboratory animal White Rabbit.Body looks into animal no abnormality seen.
Environmental condition: room temperature: 22 DEG C-25 DEG C, relative humidity: 60%-70%;
Test method:
Test according to " cosmetics health specification " (version in 2007) acute skin irritation, test first 24 hours, will test
Back part of animal spinal column diamond wool is cut, unhairing scope 3 centimetres * 3 centimetres.Take 0.5 gram of tested cosmetics and directly spread upon right side skin
On skin, cover, with nonirritant immobilization with adhesive tape 2 hours with clean gauze.Other side is negative control.After removing tested material
Within 1 hour, 24 hours, 48 hours 72 hours, observe animal subject contact site dermoreaction and keep a record.
Conclusion:
Under this experimental condition, White Rabbit acute skin irritation test is stung by the cosmetics that tested material contains polypeptide by skin
Sharp strength grading is nonirritant.Comparison cosmetics also nonirritant.
Humidity Detection protected by embodiment 6 cosmetics
MMV (moisturemeasurementvalue) method detection MMV value reflection keratodermatitis combines the ability of water.Adopt
Hydration with CM825 type skin oil-water soda acid tester test skin.Its measuring principle is owing to horny layer contains greatly
Amount electrolyte, there is the electrolyte in water and there is electric conductivity, this phenomenon also produces at skin surface, by test probe with
After contact skin, the change of electrical conductivity reflects the hydration status of skin.
Experimenter is healthy without dermopathic volunteer.Experimental condition is that the temperature and humidity of environment is constant during test,
Temperature is 25 DEG C ± 2 DEG C, and relative humidity is 40%~60%.
Choose healthy without dermopathic volunteer 28, test.Method of testing is: 1. determine the survey of 4 pieces of left and right forearm
Examination region, region area is 4cmX4cm, tests the blank value in this region respectively.2. sample is coated on test zone, and consumption is
2mg/cm2.3. after using, 120min carries out final test, 5 points of every piece of domain test, averages.Moistening effect is evaluated
Judgement schematics: in relatively moisturizing rate/%=(P sample t=x-P is blank)/(P mark t=x-P is blank) X100% formula: P sample t=x-
The electrical conductivity of product after use certain time;P is blank-it is not coated with the electrical conductivity of sample;P mark t=x-uses reference after certain time
The electrical conductivity of thing, moisturizing rate situation (%) result that the cosmetics of the present invention and glycerol compare is as shown in the table:
In terms of above table, the moisturizing rate of the cosmetics of the present invention does not has apparently higher than glycerol and commercially available cosmetics yet ratio
The moisturizing rate of the cosmetics adding polypeptide wants height, and this also illustrates that the polypeptide of the present invention is for improving the moistening effect of cosmetics simultaneously
There is significant facilitation.
Embodiment 7 protective skin cream whitening effect detects
Selecting deeper each 28 of the men and women volunteer of the colour of skin, cosmetics to the right face present invention after face cleaning, are carried out sooner or later
Sooner or later being spaced smearing of 12h, left face, as comparison, only smears the cosmetics of embodiment 4, surrounding on probation, fills out according to sensory evaluation
Write evaluation table on probation.Best result 4 is divided into effect notable, minimum is divided into 0, and contrast effect is inconspicuous.Card is utilized respectively in 4th week
Fang Jinhang adds up.
Being found by detection, the cosmetics containing polypeptide are after employing surrounding, and right face occurs in that obvious whitening effect.
The data above explanation present invention has effect of whitening.And the left and right face that right face uses glycerol is not significantly different from, illustrate
The cosmetics of invention have more significantly white-skinned face function.Also better than the effect of commercially available skin-lightening cosmetic.
The impact on A375 and Hacat co-cultured cell melanin genesis of the embodiment 8MGMB polypeptide
The ratio of A375 cell and Hacat cell 1:2 being mixed, adjusting cell density is that 104/mL of 4.5X is seeded in 6
In orifice plate, every hole 2mL, under 37 DEG C and 5%CO2, hatch 24h.Culture fluid is abandoned in suction, chooses 150ug/ according to MTT experiment result
MLMGMB polypeptide, every hole 2mL, continues to hatch 48h, removes culture fluid, with PBS 2 times, inhale and abandon culture fluid, add 0.25%
After the digestion of pancreatin 1mL/ hole, add 1mLMEM culture fluid and stop digestion, make cell suspension, cell suspension is collected 15mL and is centrifuged
Guan Zhong, centrifugal, abandon supernatant, adding 100uL concentration is lmol/L Na0H, 37 DEG C of water-bath lh, and abundant cell lysis and dissolving are black
Crude granule.Adding 400uL distilled water and release dilute, fully after mixing, add to 96 orifice plates respectively with 1OOuL/ hole, often group sets 4 multiple holes,
Each hole OD value is measured at 490nm wavelength.Melanin content A490 value represents (the results are shown in Table).Melanin genesis suppression ratio=[1_ is (real
Test the meansigma methods of group OD value/cell number)/(meansigma methods of negative control group OD value/cell number)] X 100%.With salvianolic acid phase
With concentration as comparison.
Product category |
Suppression ratio (%) |
Salvianolic acid |
60.3 |
MGMB-1 polypeptide |
94.5 |
MGMB-2 polypeptide |
95.3 |
MGMB-3 polypeptide |
94.6 |
MGMB-4 polypeptide |
95.0 |
MGMB-5 polypeptide |
95.3 |
MGMB-6 polypeptide |
94.6 |
MGMB-7 polypeptide |
94.8 |
MGMB-8 polypeptide |
94.7 |
MGMB-9 polypeptide |
95.3 |
MGMB-10 polypeptide |
95.6 |
MGMB-11 polypeptide |
95.3 |
MGMB-12 polypeptide |
94.6 |
MGMB-13 polypeptide |
95.1 |
MGMB-14 polypeptide |
95.2 |
MGMB-15 polypeptide |
95.3 |
MGMB-16 polypeptide |
96.0 |
MGMB-17 polypeptide |
95.6 |
MGMB-18 polypeptide |
96.7 |
MGMB-19 polypeptide |
95.4 |
MGMB-20 polypeptide |
94.0 |
MGMB-21 polypeptide |
95.3 |
MGMB-22 polypeptide |
94.3 |
MGMB-23 polypeptide |
93.5 |
MGMB-24 polypeptide |
94.8 |
MGMB-25 polypeptide |
95.7 |
Result shows: the polypeptide that cosmetics of the present invention are used just have than salvianolic acid more preferable melanin genesis is all had press down
Make and use.Can be good at suppressing melanic formation in Skin Cell, and then avoid the formation of new mottle, thus continue
Whitening.
Embodiment 9 anti-acne effect is verified
1, Subject Population: totally 28
1) age is between 18 to 45 years old;
2) energy compatibility test person very well, can keep regularity in one's life during studying;
3) 25 slight/moderate acne skin, 5 normal skins, totally 30.
2, method of testing: 1) position on probation: face, make the cosmetics prepared for embodiment 2-4 that apply some make up.
2) test method: at experiment septum reset left and right cheekbone, nose and pupil intersection, left and right cheek, temple are handed over the corners of the mouth
At line, left and right sides is as pilot region.Specification experimenter or care-giver adhere to using every day sooner or later, half face test sample, and half
Open face and only use clear water wiping.Experiment starts, within 2 weeks, 4 weeks, follows up a case by regular visits to, and test uses product and uses the skin of clear water respectively
Moisture, fat content, melanin and red pigment change, the test of same volunteer is completed by same survey crew.
3, search time: whole research 28 days by a definite date, researcher carried out clinical test results comment at the 0th day, 14 days, 28 days
Estimate.
4, testing standard:
1) the determining of skin oil and fat content: use skin oil and fat content measuring instrument Sebumeter to survey and average for 3 times.
2) dermal melanin and the mensuration of red pigment: use dermal melanin and red pigment tester MexameterMX18
Survey and average for 3 times.
Result shows, uses the said goods 28 days, it appeared that: use the light of the cosmetics that embodiment 2 and 3 prepares
Degree/moderate acne skin crowd, glossy problem improves significance 98.7%, and corse sweat pore improves significance 99.3%, acnitis
Disease improves significance 99.8%, and pigmentation improves significance 98.5%.And the effect of these 25 peptide species cosmetics is similar.And
The cosmetics oil optical issue of embodiment 4 and pigmentation aspect poor effect, particularly pigmentation improvement rate only have 5% left side
The right side, almost without effect.
Above content is to combine the progressive detailed description made for the present invention of concrete preferred implementation, it is impossible to assert
Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of present inventive concept, it is also possible to make some simple deduction or replace, all should be considered as belonging to the present invention's
Protection domain.
Sequence table
< 110 > Meng Fan is good
< 120 > mono-kind has the cosmetics of moisturizing whitening acne-removal function
〈160〉25
〈210〉1
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-1
QNAPWCREEKQHCNVWST
〈210〉2
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-2
KHTVERAQSQHVYKRFHW
〈210〉3
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-3
KTDSHTDFFCSGWHYWPD
〈210〉4
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-4
NYHMKRERHCSFPAKAIW
〈210〉5
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-5
DYLEVFSIFNHHVRPIQW
〈210〉6
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-6
RTRRHWITTSKKRHSMPQ
〈210〉7
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-7
MCDVHPVHTNEIWWSSPR
〈210〉8
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-8
MSKQRWTSQSYPSPTFCG
〈210〉9
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-9
HWKTIDMTMVNSWMCPAR
〈210〉10
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-10
ENYISRQTPWHYHIWRVD
〈210〉11
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-11
CTGSRINWGHSEYQWDSK
〈210〉12
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-12
QVNAQQRVVYRCHCWDSQ
〈210〉13
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-13
EPWNPANLPFPIKRVQSW
〈210〉14
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-14
LRWVSHDNGLKHGGMRER
〈210〉15
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-15
MVFYPCHTDSPTMWSNIT
〈210〉16
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-16
ANGTQSREKEHESWCMHP
〈210〉17
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-17
SRSWNDANDPWMRQQTKL
〈210〉18
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-18
NFNQMNLCKPSLNRHQCK
〈210〉19
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-19
HPDCYYFSCEATRASQIF
〈210〉20
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-20
PLAWWWQNARPDSHTWAF
〈210〉21
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-21
DPAAPTTAIEQWTIITTN
〈210〉22
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-22
RWIRRGSWPPHTDWAHKA
〈210〉23
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-23
RMKAESRWERFQGHWPIP
〈210〉24
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-24
PSDRWTMVAWQANDSRRK
〈210〉25
〈211〉18
〈212〉PRT
< 213 > artificial sequence
〈400〉MGMB-25
SRLSSSPVASKFNSVRSS