CN106072133B - A kind of preparation method with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder - Google Patents
A kind of preparation method with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder Download PDFInfo
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- CN106072133B CN106072133B CN201610481333.XA CN201610481333A CN106072133B CN 106072133 B CN106072133 B CN 106072133B CN 201610481333 A CN201610481333 A CN 201610481333A CN 106072133 B CN106072133 B CN 106072133B
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of preparation methods with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder, comprising the following steps: (1) pretreatment of mulberry fruit, blueberry and Cranberry；(2) wet pulverizing；(3) sterilizing and fermentation；(4) prepared by auxiliary material；(5) dry, packaging and sterilizing.This method reduces raw material bonding and the coking in process, on the other hand passes through the scientific compatibility and process optimization of auxiliary material, retain the bioactive substance in fresh berry to the maximum extent on the one hand by the natural carbohydrate content in microbial fermentation degradation raw material.The invention also discloses have the application in anti-oxidant and lipid-metabolism immunoregulation function health food in preparation with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder and the compound berry fine powder using made of the above method.
Description
Technical field
The invention belongs to technical field of agricultural product process, and in particular to one kind has anti-oxidant and lipid-metabolism adjustment effect
Compound berry fine powder preparation method.
Background technique
Recently as the fast development and continuous improvement of people's living standards of social economy, with oxidative stress and lipid
Metabolic disorder is that the disease incidence of the chronic diseases such as obesity, diabetes and the metabolic syndrome of common pathological characters rises rapidly.Mesh
Preceding whole world diabetic is more than 300,000,000, and the illness rate of obesity and metabolic syndrome is near or above 20%, to correct body oxygen
Change stress be the important control strategy of such disease with the diet intervention that disorders of lipid metabolism is target.Compared with developed countries,
Special food base-material or function of the China for patients' pathological characters such as fat, diabetes and metabolic syndrome and metabolic characteristic research and development
Energy food is less, and processing technology relatively lags behind, and is not able to satisfy the demand in Community health market.
Mulberry fruit is mainly distributed on China, is that health ministry ratifies " integration of drinking and medicinal herbs " food.Mulberry fruit eats sweet mouthfeel raw
It is palatable, tasteful, and have liver-kidney tonifying, fluid dryness, inducing diuresis for removing edema and a variety of medical actives such as make light of one's life by commiting suicide.Modern scientific research
Show the bioactive substances such as mulberry fruit anthocyanidin rich in, procyanidine, polyhydroxylated alkaloid and phenolic acid, they are mostly
Belong to the natural anti-oxidant and lipid-metabolism regulatory function factor.
Blueberry is the plant that one kind is distributed in the Northern Hemisphere (cold) Temperate Region in China, in addition to sugar abundant, protein, vitamin,
Outside the nutritional ingredients such as mineral element, also containing the bioactive substances such as a large amount of flavonoids, anthocyanidin, phenolic acid, have it is anti-oxidant,
The multiple functions such as hypoglycemic, reducing blood lipid, anti-inflammatory, raising immunity of organisms and enhancement eyesight, are classified as people by FAO (Food and Agriculture Organization of the United Nation)
One of big healthy food of class five.
Cranberry is a kind of fresh fruit, has the advantages that moisture is high, heat is low, is rich in multivitamin and minerals, by
The favor of people.The chemical constitution study of Cranberry the result shows that, it is a kind of rich in procyanidine and anthocyanidin and red sandalwood
The healthy berry of the various bioactivators such as stilbene, resveratrol, ellagic acid and ursolic acid, for inflammation, obesity and diabetes
With preferable prevention and auxiliary therapeutic action.
Although bioactive substances such as anthocyanidin, procyanidine, polyhydroxylated alkaloid and phenolic acid in above-mentioned 3 kinds of berries,
For the crowds such as fat, diabetes and metabolic syndrome common pathological characters --- oxidative stress and disorders of lipid metabolism have
Good prevention and auxiliary therapeutic action, but this 3 kinds of berries also contain certain carbohydrate or carbohydrate content, are unsuitable for
It is used directly to patient or is processed into relevant health food, and the presence of these carbohydrate also results in these berries
Bonding and coking occur during high temperature drying or ambient ground for raw material, influence the flavor quality and healthcare function of product.Mesh
Preceding some routine techniques mulberry fruit, blueberry and Cranberry are heated, be concentrated or crushed etc. bonding coking during processing,
The technical problems such as anthocyanidin degradation, bioactive substance loss and exterior quality deterioration, are not solved effectively always, serious to limit
Extensive use of this 3 kinds healthy berries in functional food is made.
Summary of the invention
The purpose of the present invention is to provide a kind of with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder
Preparation method, this method on the one hand by microbial fermentation degrade berry raw material in natural carbohydrate or carbohydrate content,
Raw material bonding and the coking in process are reduced, on the other hand passes through the scientific compatibility and process optimization of auxiliary material, to greatest extent
Ground retains the bioactive substance adjusted in fresh berry with anti-oxidant and lipid-metabolism.
The object of the invention is also to provide utilize to have anti-oxidant and lipid-metabolism adjustment effect made of the above method
Compound berry fine powder, or with the products such as nutrition bar that the compound berry fine powder is main Raw material processing, such product is rich in original
The active skull cap components such as anthocyanidin, anthocyanidin, polyhydroxylated alkaloid and phenolic acid, and pass through the excellent of processing technology and auxiliary material combination
Change can promote product maintenance to have bright-coloured appearance luster.
Final object of the present invention is that providing above-mentioned compound berry fine powder has anti-oxidant and lipid generation in preparation
Thank to the application in the health food of immunoregulation function.
The first purpose of this invention is achieved through the following technical solutions: one kind having anti-oxidant and lipid-metabolism
The preparation method of the compound berry fine powder of adjustment effect, comprising the following steps:
(1) pretreatment of mulberry fruit, blueberry and Cranberry: choosing fresh mature mulberry fruit, blueberry and Cranberry, pre-processes standby
With；
(2) wet pulverizing: choosing mulberry fruit, blueberry and Cranberry after pretreatment, after colour protecting liquid mixing is added, is ground using grinding wheel
Secondary wet process crushing is carried out with colloid mill, it is spare to obtain composite pulp fruit protoplasm；
(3) sterilizing and fermentation: composite pulp fruit protoplasm is cooling after microwave sterilization, it is first inoculated with by lactobacillus plantarum and goldbeater's skin
The composite bacteria fermentation of leukonid composition, obtains leading portion tunning, inoculates saccharomyces cerevisiae and continue to ferment, work as tunning
In when no longer detecting glucose and fructose, obtain back segment tunning；
(4) prepared by auxiliary material: choosing auxiliary material, is ground after soaking using grinding wheel and colloid mill carries out secondary wet process crushing, preparation
Pulp auxiliary material is spare；
(5) dry, packaging and sterilizing: auxiliary material and back segment tunning are mixed, through drying, packaging and sterilization treatment, i.e.,
It is prepared into anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder.
The present invention has the preparation method of anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder, with fresh mature
Mulberry fruit, blueberry and Cranberry be primary raw material, through include pretreatment, wet pulverizing, sterilizing, inoculation fermentation (lactic acid bacteria, yeast
Two sections of bacterium fermentations), auxiliary material addition, the processes such as dry, packaging and sterilizing prepare.
The preparation method is complete under room temperature or cryogenic conditions by microbiological transformation technology and atomizing freeze drying technology
At can effectively avoid procyanidine, anthocyanidin, polyhydroxylated alkaloid and phenolic acid in mulberry fruit, blueberry and Cranberry etc. naturally living
The degradation or variation of property ingredient, and the appearance luster for maintaining product bright-coloured.
In the above-mentioned preparation method with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder:
Pretreatment as described in step (1) is spare including cleaning, sterilizing, cleaning and being filtered dry.
The wherein removal of impurities, disinfection, cleaning and draining process of mulberry fruit, blueberry and Cranberry are as follows: it is ripe fresh to select eight, ninety percent
Mulberry fruit, blueberry and Cranberry (bright in colour and without go rotten), remove the naked eyes visible foreigns such as blade, snail, cleaning uses by hand
It is spare that the cleaning of ultrasonic bubble method is filtered dry rear freezing.
Further, pretreated detailed process is in step (1): selecting eight, ninety percent ripe mulberry fruit, blueberry and Cranberry
(bright in colour and without go rotten), after removing macroscopic impurity, with clear water or with meet provide (GB5461) 0.5%~
0.8% edible salt water solution or other disinfectants for meeting national Specification carry out surface to mulberry fruit, blueberry and Cranberry and disappear
Poison, then be cleaned multiple times with pure water, except desalting or going disinfectant, the berry being filtered dry is cleaned through food-grade compound film packing material packet
After dress, it is spare to set -18 DEG C of freezer chilled storages, when production, takes out thaw after use.
The proportion by weight of mulberry fruit, blueberry and Cranberry described in step (2) is 3 ~ 5:1 ~ 2:0.5 ~ 1.
Colour protecting liquid described in step (2) is citric acid and sodium isoascorbate, the dosage of citric acid be mulberry fruit, blueberry and
The 0.10% ~ 0.20% of Cranberry gross mass, the dosage of sodium isoascorbate be mulberry fruit, blueberry and Cranberry gross mass 0.01% ~
0.02%。
When being crushed in step (2) and step (4) using colloid mill, control equipment gap size is 25 ~ 40 μm, grinds number
It is 2 ~ 4 times, processed in units amount 4000g/min.
When wherein carrying out secondary wet process crushing using grinding wheel mill and colloid mill in step (2), for the first time using grinding wheel mill pair
Mulberry fruit, blueberry and Cranberry carry out preliminary so broken that mix magma；Second using colloid mill to the mixing further fine grinding of magma,
It is spare to obtain composite pulp fruit protoplasm freezen protective.
Wherein colloid mill being added through tentatively broken mixing magma second, control equipment gap size is 25-40 μm,
It is spare to obtain -18 DEG C of freezen protectives of composite pulp fruit protoplasm for grinding number 2-4 times, processed in units amount 4000g/min.
In step (3) when microwave sterilization, the power of micro-wave oven is preferably 400 ~ 500W, and the microwave time is preferably 150 ~
210s。
Specifically, first the composite pulp fruit protoplasm of freezen protective thaws in step (3), then preferably killed using pipeline microwave
Bacterium machine is sterilized, and when microwave disinfection, the power of micro-wave oven is preferably 400 ~ 500W, and the microwave time is preferably 150 ~ 210s.
After microwave disinfection is cooling in step (3), first inoculating lactic acid bacterium carries out aerobic fermentation, is then inoculated with saccharomyces cerevisiae and continues
Aerobic fermentation is carried out, until can not detect in tunning terminates to ferment when glucose and fructose.
It is former that the inoculum concentration for the composite bacteria being made of in step (3) lactobacillus plantarum and Leuconostoc mesenteroides accounts for compound berry
The 10 ~ 12% of gross mass are starched, wherein the initial concentration of lactobacillus plantarum and Leuconostoc mesenteroides is respectively 1.0 × 108~2.0×108
The volume ratio of cfu/mL, lactobacillus plantarum and Leuconostoc mesenteroides is 2 ~ 3:1 ~ 1.5, and sterile purified water adjustment liquid is added after inoculation
Material is than being 1.25 ~ 1.60:1, and control fermentation temperature is 40 ± 2 DEG C, and fermentation time is 48 ~ 96h, obtains leading portion tunning,
The inoculum concentration of saccharomyces cerevisiae accounts for the 0.10 ~ 0.20% of leading portion total mass of fermentation products, when inoculation saccharomyces cerevisiae continues fermentation, control
Fermentation temperature be 40 ± 2 DEG C, fermentation time be 12 ~ for 24 hours.
Lactobacillus plantarum, Leuconostoc mesenteroides and saccharomyces cerevisiae are commercial product in the present invention.
Auxiliary material described in step (4) is one or more of soya bean, buckwheat and oat, and the dosage of water is soya bean, buckwheat
With 4 ~ 8 times of oat gross mass, soaking time be 8 ~ 12h.
When the auxiliary material is soya bean and buckwheat, the proportion by weight of the soya bean and buckwheat is 10 ~ 20:3 ~ 4；The auxiliary material
When for soya bean, buckwheat and oat, the proportion by weight of the soya bean, buckwheat and oat is 10 ~ 20:3 ~ 4:2 ~ 5.
The proportion by weight of auxiliary material and back segment tunning is 1 ~ 3:5 ~ 10 in step (5), dry dry using spray chilling
Dry, the dry water content to material is 8 ~ 10%.
Further, in step (5) it is dry, pack and the detailed process of sterilizing be by auxiliary material and back segment tunning by 1 ~
The proportion by weight of 3:5 ~ 10 after mixing, is added in atomizing freeze drying equipment, controls -60 DEG C of condenser temperature, vacuum
Spend 20Pa, -25 DEG C of temperature of charge, through vacuum freeze drying to material moisture 8 ~ 10%, as compound berry fine powder.
Second object of the present invention is achieved through the following technical solutions: anti-using having made of the above method
The compound berry fine powder of oxidation and lipid-metabolism adjustment effect.
Third object of the present invention is achieved through the following technical solutions: above-mentioned compound berry fine powder has in preparation
There is the application in anti-oxidant and lipid-metabolism immunoregulation function health food.
Pharmacy test shows compound berry fine powder provided by the invention, can further mix with other auxiliary materials, be developed into tool
There is anti-oxidant and lipid-metabolism adjustment effect nutraceutical (health food), such as nutrition bar and meal replacement powder, so as to stable state
Change regulation patient's oxidative stress or disorders of lipid metabolism is horizontal.
Compared with prior art, the present invention has the advantage that
(1) primary raw material that the present invention selects is in nature rich in procyanidine, anthocyanidin, polyhydroxylated alkaloid and phenol
The healthy berries of three kinds of acid: mulberry fruit, blueberry and Cranberry, selected auxiliary material are also to be suitable for oxidative stress and lipid-metabolism is disorderly
The full cereal such as oat, buckwheat and soya bean of random edible for patients or bean food, with the foodstuff base material of these raw materials exploitation for steady
Stateization, which regulates and controls the oxidative stress of patient and disorders of lipid metabolism, has important health promotion effect；
(2) present invention be used in combination lactobacillus plantarum, Leuconostoc mesenteroides and saccharomyces cerevisiae to three kinds of berry mixtures into
Row deep fermentation twice, on the one hand can carbohydrate content in degradable berry, while promoting some combinations in berry
The release of the glycolipid metabolisms function factor such as phenolic acid and polyhydroxylated alkaloid in fiber, produces during above-mentioned probiotics fermention
A large amount of probiotics is given birth to, blood glucose and the blood lipid level for adjusting glucose -lipid metabolism disorder patient are also very useful；
(3) auxiliary material oat, buckwheat and the soya bean etc. that the present invention selects are to adjust active healthy paddy with glycolipid metabolism
Material can be bonded together in spray-freeze dry process by object or beans by reasonably matching, promote product at
Type, it is of particular importance that the stability of anthocyanidin and the survival rate of probiotics can be significantly improved by adding above-mentioned auxiliary material mixture,
Appearance luster and bioactivity for product have very important protective effect, can by the processing technology of this stable state
To obtain the fine powder raw material that bright in colour, viscosity is small and mobility is splendid；
(4) processing technology of the present invention controls substantially completes under room temperature or cryogenic conditions, efficiently avoids starching
The variation of the active skull cap components such as fruit procyanidins, anthocyanidin, polyhydroxylated alkaloid and phenolic acid, maintain mulberry fruit, blueberry and
Nutrition, flavor and the functional components of 3 kinds of berries of Cranberry；
(5) therefore, the method for the present invention reduces and adds on the one hand by the natural carbohydrate content in microbial fermentation degradation raw material
Raw material bonding and coking during work, on the other hand pass through the scientific compatibility and process optimization of auxiliary material, retain to the maximum extent
Bioactive substance in fresh berry.
Specific embodiment
Following embodiment belongs to the protection scope of invention, the common skill of the art for illustrating and implementing the present invention
The purpose of the present invention can be achieved in the content of art personnel according to the above disclosure.
Embodiment 1
Preparation method provided in this embodiment with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder, packet
Include following steps:
(1) pretreatment of mulberry fruit, blueberry and Cranberry selects eight, ninety percent ripe fresh mulberry fruit, blueberry and Cranberry (color
Pool is bright-coloured and without mildew and rot), the naked eyes visible foreigns such as blade, snail are removed by hand, provide (GB5461) with clear water or with meeting
0.5%~0.8% edible salt water solution or other disinfectants for meeting national Specification carry out surface sterilization to fruit, then use
Ultrasonic bubble method ablution is by salt or disinfectant is gone to clean up, and is filtered dry spare.
(2) wet pulverizing of mulberry fruit, blueberry and Cranberry is by mulberry fruit, blueberry and Cranberry according to the mass ratio of 3:1:1
It weighs and mixes, 0.10% citric acid is added in ratio (in terms of mulberry fruit, blueberry and Cranberry gross mass) by weight and 0.02 % is different anti-
Bad hematic acid sodium carries out color protection, the 1st time using grinding wheel grind by mixed mulberry fruit, blueberry and Cranberry carry out it is tentatively broken after obtain
Mix magma；2nd time will be through tentatively broken mixing magma addition colloid mill, and control equipment gap size is 40 μm, grinding time
Number 2 times, processed in units amount 4000g/min, it is spare to obtain composite pulp fruit protoplasm.
(3) it sterilizes, be inoculated with and ferment for composite pulp fruit protoplasm to be placed in pipeline microwave sterilization machine and control microwave power
500w, microwave time 150s carry out sterilization treatment.First inoculating lactic acid bacterium compound bacteria (lactobacillus plantarum and the bright string of goldbeater's skin after cooling
Pearl bacterium) fermentation, the inoculum concentration of the composite bacteria being made of lactobacillus plantarum and Leuconostoc mesenteroides is 10%(with compound after sterilizing
Berry magma gross mass meter), added after inoculation sterile purified water adjustment liquid-to-solid ratio be 1.25:1, control fermentation temperature be 40 ±
2 DEG C, fermentation time 48h, leading portion tunning is obtained, wherein the initial concentration of lactobacillus plantarum and Leuconostoc mesenteroides point
It Wei 1.0 × 108 cfu/mL、2.0×108The volume ratio of cfu/mL, lactobacillus plantarum and Leuconostoc mesenteroides is 2:1.5；After
And be inoculated with saccharomyces cerevisiae and continue to ferment, the inoculum concentration of saccharomyces cerevisiae is 0.10%(in terms of leading portion tunning), control fermentation temperature
It is 40 ± 2 DEG C, fermentation time is for 24 hours, until fermentation ends obtain compound berry back segment tunning, lactobacillus plantarum, goldbeater's skin
Leukonid and saccharomyces cerevisiae are commercial product.
(4) auxiliary material allotment auxiliary material is made of soya bean and buckwheat by weight 10:3 mixing, and 5 times of pure water is added and impregnates
12h first be tentatively crushed through grinding wheel mill, and colloid mill then is added in mixed accessories, and control equipment gap size is 40 μm, grinds
Mill number 2 times, processed in units amount 4000g/min obtains soya bean buckwheat and mixes magma.
(5) compound berry second stage (back segment) tunning is mixed magma with soya bean buckwheat and pressed by atomizing freeze drying
Proportion by weight be 5:3 after mixing, be added atomizing freeze drying equipment, control -60 DEG C of condenser temperature, vacuum degree 20Pa,
It is -25 DEG C of temperature of charge, dry to material moisture 8 ~ 10%, the as compound berry fine powder of Sugarless type.
(6) packing will dry with sterilizing to the edible clad aluminum foil of the compound berry fine powder of Sugarless type of water content 8 ~ 10%
Bag packaging, labels after microwave sterilization and obtains finished product after the assay was approved.
Embodiment 2
Processing method provided in this embodiment with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder, packet
Include following steps:
(1) pretreatment of mulberry fruit, blueberry and Cranberry selects eight, ninety percent ripe fresh mulberry fruit, blueberry and Cranberry (color
Pool is bright-coloured and without mildew and rot), the naked eyes visible foreigns such as blade, snail are removed by hand, provide (GB5461) with clear water or with meeting
0.8% edible salt water solution or other disinfectants for meeting national Specification carry out surface sterilization to fruit, then with ultrasound gas
Bubble method ablution is by salt or disinfectant is gone to clean up, and is filtered dry spare.
(2) wet pulverizing of mulberry fruit, blueberry and Cranberry is by mulberry fruit, blueberry and Cranberry according to the ratio of 4:2:0.5
It weighs and mixes, 0.20% citric acid is added in ratio by weight and 0.015 % sodium isoascorbate carries out color protection, and the 1st time using sand
Disc sharpener carries out mixed mulberry fruit, blueberry and Cranberry to obtain mixing magma after being tentatively crushed；2nd time will be through tentatively broken
It mixes magma and colloid mill is added, control equipment gap size is 30 μm, is ground number 3 times, processed in units amount 4000g/min is obtained
It is spare to obtain composite pulp fruit protoplasm.
(3) it sterilizes, be inoculated with and ferment for composite pulp fruit protoplasm to be placed in pipeline microwave sterilization machine and control microwave power
450w, microwave time 180s carry out sterilization treatment.First inoculating lactic acid bacterium compound bacteria (lactobacillus plantarum and the bright string of goldbeater's skin after cooling
Pearl bacterium) fermentation, the inoculum concentration for the composite bacteria that lactobacillus plantarum and Leuconostoc mesenteroides form is 11%, and sterilizing is added after inoculation
It is 1.4:1 that distilled water, which adjusts liquid-to-solid ratio, and control fermentation temperature is 40 ± 2 DEG C, fermentation time 60h, obtains leading portion fermentation and produces
Object, wherein the concentration of lactobacillus plantarum and Leuconostoc mesenteroides is respectively 1.5 × 108 cfu/mL、1.5×108Cfu/mL plants
The volume ratio of object lactobacillus and Leuconostoc mesenteroides is 3:1；Then inoculation saccharomyces cerevisiae continues to ferment, the inoculum concentration of saccharomyces cerevisiae
It is 0.15%, control fermentation temperature is 40 ± 2 DEG C, fermentation time 12h, until fermentation ends obtain the back segment hair of compound berry
Ferment product.Lactobacillus plantarum, Leuconostoc mesenteroides and saccharomyces cerevisiae are commercial product in the present invention.
(4) auxiliary material allotment auxiliary material is made of soya bean, buckwheat and oat by weight 15:3:3 mixing, and 8 times pure is added
Water impregnates 10h, first be tentatively crushed through grinding wheel mill, and colloid mill then is added in mixed accessories, and control equipment gap size is
It 30 μm, grinds number 3 times, processed in units amount 4000g/min, obtains mixed accessories magma.
(5) compound berry back segment tunning and mixed accessories magma are 7:2 by proportion by weight by atomizing freeze drying
After mixing, atomizing freeze drying equipment is added, controls -60 DEG C of condenser temperature, vacuum degree 20Pa, -25 DEG C of temperature of charge, does
It is dry to material moisture 8 ~ 10%, as compound berry fine powder.
(6) packing will dry with sterilizing to the edible clad aluminum foil bag packet of the compound berry fine powder of water content 8 ~ 10%
Dress labels after microwave sterilization and obtains finished product after the assay was approved.
Embodiment 3
Preparation method provided in this embodiment with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder, packet
Include following steps:
(1) pretreatment of mulberry fruit, blueberry and Cranberry.Select eight, ninety percent ripe fresh mulberry fruit, blueberry and Cranberry (color
Pool is bright-coloured and without mildew and rot), the naked eyes visible foreigns such as blade, snail are removed by hand, provide (GB5461) with clear water or with meeting
0.5%~0.8% edible salt water solution or other disinfectants for meeting national Specification carry out surface sterilization to fruit, then use
Ultrasonic bubble method ablution is by salt or disinfectant is gone to clean up, and is filtered dry spare.
(2) wet pulverizing of mulberry fruit, blueberry and Cranberry.By mulberry fruit, blueberry and Cranberry according to the matter of 5:1.5:0.8
Amount ratio is weighed and is mixed, and 0.15% lemon is added by mixing berry weight ratio (in terms of mulberry fruit, blueberry and Cranberry gross mass)
Acid and 0.01 % sodium isoascorbate carry out color protection, the 1st time using grinding wheel grind by mixed mulberry fruit, blueberry and Cranberry carry out
Mixing magma is obtained after being tentatively crushed；2nd time will be through tentatively broken mixing magma addition colloid mill, control equipment gap size
It is 25 μm, grinds number 4 times, processed in units amount 4000g/min, it is spare obtains composite pulp fruit protoplasm.
(3) it sterilizes, be inoculated with and ferment.Composite pulp fruit protoplasm is placed in pipeline microwave sterilization machine and controls microwave power
400w, microwave time 210s carry out sterilization treatment.First inoculating lactic acid bacterium compound bacteria (lactobacillus plantarum and the bright string of goldbeater's skin after cooling
Pearl bacterium) fermentation, the inoculum concentration of the composite bacteria being made of lactobacillus plantarum and Leuconostoc mesenteroides is 12%(with compound after sterilizing
Berry magma gross mass meter), it is 1.6:1 that sterile purified water adjustment liquid-to-solid ratio is added after inoculation, and control fermentation temperature is 40 ± 2
DEG C, fermentation time 96h obtains leading portion tunning, and wherein the initial concentration of lactobacillus plantarum and Leuconostoc mesenteroides is distinguished
It is 2.0 × 108 cfu/mL、1.0×108The volume ratio of cfu/mL, lactobacillus plantarum and Leuconostoc mesenteroides is 2.5:1.2；After
And be inoculated with saccharomyces cerevisiae and continue to ferment, the inoculum concentration of saccharomyces cerevisiae is 0.2%(in terms of leading portion tunning), control fermentation temperature
It is 40 ± 2 DEG C, fermentation time 18h, until fermentation ends obtain compound berry back segment tunning, lactobacillus plantarum, goldbeater's skin
Leukonid and saccharomyces cerevisiae are commercial product.
(4) auxiliary material is deployed.Auxiliary material is oat, and 6 times of pure water is added and impregnates 8h, first be tentatively crushed through grinding wheel mill,
Then colloid mill is added in mixed accessories, control equipment gap size is 25 μm, is ground number 4 times, processed in units amount 4000g/
Min obtains soya bean buckwheat and mixes magma.
(5) atomizing freeze drying.Compound berry back segment tunning is mixed into magma by proportion by weight with soya bean buckwheat
After mixing for 10:1), atomizing freeze drying equipment is added, controls -60 DEG C of condenser temperature, vacuum degree 20Pa, temperature of charge -
It is 25 DEG C, dry to material moisture 8 ~ 10%, the as compound berry fine powder of Sugarless type.
(6) packaging and sterilizing.It will dry to the edible clad aluminum of the compound berry fine powder of Sugarless type of water content 8 ~ 10%
Foil bag packaging, labels after microwave sterilization and obtains finished product after the assay was approved.
Embodiment 4 has anti-oxidant and lipid-metabolism adjustment effect generation meal nutrition bar preparation process
(1) auxiliary material preparation mixing.By oatmeal, whey protein concentrate, soybean protein isolate, oligoisomaltose and walnut
5 kinds of auxiliary materials such as benevolence are pressed after proportion by weight 5:0.5:1:0.2:1 is weighed after mixing, in addition weigh camellia by weight 30:1
Oil is slowly added into mixed accessories and stirs 5-6min to more uniform state.
(2) supplementary material mixes.Composite effect fine powder and auxiliary material prepared by embodiment 2 is mixed by weight the ratio of 1:2
It stirs evenly.
(3) drying and moulding.The supplementary material mixed is placed in mold, abundant extrusion forming, so that surfacing, tissue
It is compact, dry 15min is finally placed in micro-wave vacuum case, and control microwave power is 1 kW, and relative pressure is -80
KPa, and with the rotation of the speed of 10 r/min.
(4) cooling and demolding.By the nutrition bar of drying and moulding after room temperature natural cooling, is packed and gone out after demoulding
Bacterium.
(5) packaging and sterilizing.The molded edible clad aluminum foil bag of generation meal nutrition bar is packed, is pasted after microwave sterilization
It marks and obtains finished product after the assay was approved.
The anti-oxidant and lipid-metabolism of the compound berry fine powder of embodiment 5 adjusts pharmacodynamics test
It is raw that 3 kinds of berries such as mulberry fruit selected by the present invention, blueberry and Cranberry contain procyanidine, anthocyanidin, polyhydroxy
The various actives function factor such as alkaloids and phenolic acid, the anti-oxidant and lipid-metabolism for having the above-mentioned function factor of document report are adjusted
Effect.Selected auxiliary material such as soya bean, oat and buckwheat are also oxidative stress and the recommendation food of disorders of lipid metabolism patient, therefore
Above-mentioned supplementary material is all widely used in the prevention and treatment of the chronic diseases such as fat, diabetes and metabolic syndrome, below
By animal efficacy test illustrate the compound berry fine powder prepared by the present invention in body oxidative stress and disorders of lipid metabolism side
The adjustment effect in face.
One, test material:
1, sample: the sample of this pharmacodynamic test is prepared by above-described embodiment 2.
2, reagent: streptozotocin (STZ) analyzes pure (Sigma)；Blood lipid level detection kit is (total triglycerides, total
Cholesterol) and blood oxidation stress level detection kit (SOD, GSH-Px, MDA and Fe3+Reducing power builds up life purchased from Nanjing
Object Graduate School of Engineering.
3, laboratory apparatus: FA1104 assay balance Shanghai balance equipment, the prosperous pump in ZL-1 type vacuum freeze drier Shanghai,
EYELA N-1001 Rotary Evaporators (Shanghai Ai Lang Instrument Ltd), UV-1700 ultraviolet specrophotometer (Japanese Shimadzu),
Microplate reader (KHB ST-360), gas bath constant-temperature table (Thermo), 96 hole elisa Plates (Costar), liquid-transfering gun (Bio-Rad).
4, experimental animal: Kun Ming mice (20 ± 2g), SPF grades, male, Guangdong Province's Experimental Animal Center provides.
Two, test method:
1, the foundation of the metabolic disorder of high fat diet inducing lipids and oxidative stress mouse model: Kunming mouse (average body
Weigh 20 ± 2g) after progress high lipid food nursing 4 weeks, fasting 12h, next day surveys fasting blood total cholesterol (TC) and triglycerides
(TG), the nmol/mL person of TC >=3.0mmol/L, TG >=0.65mmol/L, MDA >=6.5 be disorders of lipid metabolism and
Mouse in oxidative stress status.
2, animal packet and administration: the mouse of no significant difference will be examined to be randomly divided into 5 groups by weight through T: blank control
Group, model control group, sample low dose group, middle dose group and high dose group.Each group processing method are as follows: low dose group dosage
200 mg/kg·(bw)；400 mg/kg of middle dose group dosage (bw)；800 mg/kg of high dose group dosage (bw)；
Normal control and model control group give isometric physiological saline every time, oral stomach-filling 1 time daily, continue nursing 4 weeks, during which
It freely ingests drinking-water, the weight of weighing in every 3 days.
3, it tests the measurement of each index: during experiment, surveying a weight and feed intake every three days.Eye is used after experiment
Socket of the eye veniplex blood taking method takes blood, measures the indexs such as blood total cholesterol (TC) and triglycerides.
Three, data processing
Data processing and statistical analysis use 19.0 software package of SPSS, as a result with mean ± standard deviation () indicate, respectively
Comparison between group uses least significant difference method (Least Significant Difference, LSD).
Four, test result
Pass through the anti-oxidant and lipid-metabolism tune of the evaluating combined berry fine powder of glucose -lipid metabolism disorder model mice in vivo studies
Section is active, in the following table 1 the result shows that by adding this product, 2 kinds of blood of disorders of lipid metabolism and oxidative stress model mice
Fat indicators (TC, TG), 2 kinds of antioxidase indexs (SOD, GSH-Px), Fe3+Reducing power index (FRAP) and lipid peroxidation
Product index (MDA) is preferably controlled, and each index level exists aobvious with the model control group mouse for not adding this product
Sex differernce is write, close to blank group level, illustrates that this product can play preferable anti-oxidant and lipid-metabolism adjustment effect.
Anti-oxidant and lipid-metabolism index of correlation of the grain products of the present invention of table 1 to the serum of glucose -lipid metabolism disorder mouse
Improvement result
Note: different lowercases indicate that above there were significant differences in 0.05 level between the two in table.TC, total cholesterol；TG,
Total triglycerides；SOD, superoxide dismutase；GSH-Px, glutathione peroxidase；FRAP, Fe3+Reducing power；MDA,
Malonaldehyde.
The above embodiment is only for illustrating the present invention, and protection scope of the present invention is not intended to be limited to the above implementation
Example.Those skilled in the art based on the contents of the disclosure of the present invention and the scope of each parameter, can be achieved
The purpose of the present invention.
Claims (9)
1. a kind of preparation method of the compound berry fine powder with anti-oxidant and lipid-metabolism adjustment effect, it is characterized in that include with
Lower step:
(1) pretreatment of mulberry fruit, blueberry and Cranberry: choosing fresh mature mulberry fruit, blueberry and Cranberry, spare after pretreatment；
(2) wet pulverizing: choosing mulberry fruit, blueberry and Cranberry after pretreatment, after colour protecting liquid mixing is added, using grinding wheel mill and glue
Body mill carries out secondary wet process crushing, and it is spare to obtain composite pulp fruit protoplasm；
(3) sterilizing and fermentation: composite pulp fruit protoplasm is cooling after microwave sterilization, it is first inoculated with by lactobacillus plantarum and the bright string of goldbeater's skin
Pearl bacterium composition composite bacteria fermentation, obtain leading portion tunning, inoculate saccharomyces cerevisiae and continue to ferment, when in tunning not
When detecting glucose and fructose again, back segment tunning is obtained；
(4) prepared by auxiliary material: choosing auxiliary material, is ground after soaking using grinding wheel and colloid mill carries out secondary wet process crushing, preparation is slurried
Shape auxiliary material is spare；
(5) dry, packaging and sterilizing: auxiliary material and back segment tunning are mixed, through drying, packaging and sterilization treatment, that is, prepared
There must be anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder；
Auxiliary material described in step (4) is one or more of soya bean, buckwheat and oat；
It is total that the inoculum concentration for the composite bacteria being made of in step (3) lactobacillus plantarum and Leuconostoc mesenteroides accounts for composite pulp fruit protoplasm
The 10 ~ 12% of quality, wherein the initial concentration of lactobacillus plantarum and Leuconostoc mesenteroides is respectively 1.0 × 108~2.0×108
The volume ratio of cfu/mL, lactobacillus plantarum and Leuconostoc mesenteroides is 2 ~ 3:1 ~ 1.5, and sterile purified water adjustment liquid is added after inoculation
Material is than being 1.25 ~ 1.60:1, and control fermentation temperature is 40 ± 2 DEG C, and fermentation time is 48 ~ 96h, obtains leading portion tunning,
The inoculum concentration of saccharomyces cerevisiae accounts for the 0.10 ~ 0.20% of leading portion total mass of fermentation products, when inoculation saccharomyces cerevisiae continues fermentation, control
Fermentation temperature be 40 ± 2 DEG C, fermentation time be 12 ~ for 24 hours.
2. the preparation side according to claim 1 with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder
Method, it is characterized in that: the proportion by weight of mulberry fruit, blueberry and Cranberry described in step (2) is 3 ~ 5:1 ~ 2:0.5 ~ 1.
3. the preparation side according to claim 1 with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder
Method, it is characterized in that: colour protecting liquid described in step (2) is citric acid and sodium isoascorbate, the dosage of citric acid is mulberry fruit, indigo plant
The 0.10% ~ 0.20% of the certain kind of berries and Cranberry gross mass, the dosage of sodium isoascorbate are mulberry fruit, blueberry and Cranberry gross mass
0.01%~0.02%。
4. the preparation side according to claim 1 with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder
Method, it is characterized in that: control equipment gap size is 25 ~ 40 μm, grinding when being crushed in step (2) and step (4) using colloid mill
Number is 2 ~ 4 times, processed in units amount 4000g/min.
5. the preparation side according to claim 1 with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder
Method, it is characterized in that: the power of micro-wave oven is 400 ~ 500W in step (3) when microwave sterilization, the microwave time is 150 ~ 210s.
6. the preparation side according to claim 1 with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder
Method, it is characterized in that: the dosage of water is 4 ~ 8 times of auxiliary material gross mass when impregnating in step (4), soaking time is 8 ~ 12h.
7. the preparation side according to claim 1 with anti-oxidant and lipid-metabolism adjustment effect compound berry fine powder
Method, it is characterized in that: the proportion by weight of auxiliary material and back segment tunning is 1 ~ 3:5 ~ 10 in step (5), it is dry using spraying cold
It is lyophilized dry, the dry water content to material is 8 ~ 10%.
8. using smart with anti-oxidant and lipid-metabolism adjustment effect compound berry made of any one of claim 1-7 method
Powder.
9. compound berry fine powder according to any one of claims 8 has anti-oxidant and lipid-metabolism immunoregulation function health food in preparation
In application.
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| CN108741014A (en) * | 2018-04-24 | 2018-11-06 | 北京中和鸿业医药科技有限公司 | A kind of antioxidant composition, health food, preparation method and applications |
| CN111280409B (en) * | 2020-03-03 | 2023-10-13 | 怀化恒祺农业发展股份有限公司 | Method for drying berry products containing polyphenol, composite dry powder containing polyphenol and application thereof |
| CN111471604B (en) * | 2020-04-20 | 2021-09-21 | 中国农业科学院麻类研究所 | Application of saccharomyces cerevisiae ZLG-6 and lactobacillus plantarum Picp-2 |
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