CN106071592A - The preparation method of a kind of hedgehog hydnum oral liquid and special culture media - Google Patents
The preparation method of a kind of hedgehog hydnum oral liquid and special culture media Download PDFInfo
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- CN106071592A CN106071592A CN201610488076.2A CN201610488076A CN106071592A CN 106071592 A CN106071592 A CN 106071592A CN 201610488076 A CN201610488076 A CN 201610488076A CN 106071592 A CN106071592 A CN 106071592A
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- 241000577951 Hydnum Species 0.000 title claims abstract description 26
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 7
- 150000002016 disaccharides Chemical class 0.000 claims abstract description 6
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Organic Chemistry (AREA)
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- Polymers & Plastics (AREA)
- Alternative & Traditional Medicine (AREA)
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- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
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Abstract
The invention discloses preparation method and the special culture media of a kind of hedgehog hydnum oral liquid, fundamentally solve and fill up, with biofermentation technique, the present situation that wild hedgehog hydnum is not enough, also improve the content of hedgehog hydnum main component in oral liquid.Culture medium is based on natural plants, and natural green is without chemical residual, and trace element is many, comprehensive nutrition；Glucose adds jointly with the disaccharide of one of sucrose, white sugar, brown sugar, allows composition comprehensive and lasting；3, traditional chemical defoamer is substituted with vegetable oil, the most nutritious, again without chemical residual；4, oral liquid is producing and in processing pouring process, omnidistance do not use high temperature, be dried, extract, crush etc. traditional or modern either physically or chemically, the most only effectively remain all of active component in oral liquid, and make active component not be damaged or destroyed；From preparation shaking flask kind to product fill all without additives such as any preservative, thickening agent, stabilizer, coloring agent, this oral liquid active constituent content is high.
Description
Technical field
The present invention relates to the preparation method of a kind of oral liquid, the preparation method of a kind of hedgehog hydnum oral liquid.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers. cries again Hericium erinaceus (Bull. Ex Fr.) Pers., monkey mushroom, hedgehog hydnum, monkey mushroom, is Russula mesh, hedgehog hydnum Cordycepps, Hericium, Basidiomycetes.
It is a kind of saprophytic bacteria, is also a kind of famous edible fungi.
In China, hedgehog hydnum has long history.Compendium of Material Medica is recorded, and Hericium erinaceus (Bull. Ex Fr.) Pers. property is flat, sweet in the mouth, has and " profit the five internal organs, helps
Digestion " function.
Hedgehog hydnum does not contain only the nutrient substance such as protein, vitamin, inorganic salt, possibly together with polysaccharide, polypeptide, sterol, terpenoid,
The various active material such as adenosine, phenols.
Hedgehog hydnum has raising immunity of organisms, suppression gastrointestinal tumor, improves quantity of leucocyte, radioprotective, promotion Weishang skin
Cytothesis, repair gastroenteritic ulcer and inflammation, calm the nerves, spasmolytic, improve the effect such as liver function.Relevant research shows, hedgehog hydnum and
Extract is harmless to human non-toxic, even if long-term, high-dose is taken the most without any side effects.
Summary of the invention
It is an object of the invention to provide preparation method and the special culture media of a kind of hedgehog hydnum oral liquid, fundamentally solve
Fill up, with biofermentation technique, the present situation that wild hedgehog hydnum is not enough, also improve the content of hedgehog hydnum main component in oral liquid, for food
Product industry and pharmaceutical industry, the health for the people provides new guarantee and approach.
The technical scheme is that and be achieved in that: the preparation method of a kind of hedgehog hydnum oral liquid, step is as follows:
1) preparing shaking flask kind: culture medium loaded in conical flask, liquid amount 25-35%, the temperature of 121-123 DEG C after sealing
Lower sterilizing 35 minutes, when culture medium temperature drops to 20-28 DEG C, aseptically accesses slant strains in triangular flask and shakes
Bed is cultivated, and when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, mycelium pellet is high-visible, and all burrs are obvious, and microscopy is without miscellaneous
During bacterium, standby；
2) fermentor cultivation: culture medium is loaded in fermentation tank, liquid amount is the 55-75% of fermentation tank total capacity, when tank temperature rise extremely
121-123 DEG C, tank pressure 0.11-0.13Mpa time, sterilizing 50min, then lead to cooling water temperature, when temperature drops to 30 DEG C in tank
Stop, adjusting tank pressure 0.05Mpa, enter cultivation conditions；
Cultivate in cultured shaking flask kind strain is accessed tank, inoculum concentration 5-15%, tank temperature 20-28 DEG C, tank pressure 0.04-
0.05MPa, gas flow 1.5m3/ h, when cultivating 36-48h in tank, pick test；
Continuing to cultivate 5-7d, when fungus ball is high-visible, all burrs are obvious, and bacterium solution is faint yellow, about pH5-6, residual sugar amount fall
During to about 0.3%, cultivation terminates, and carries out subpackage.Described subpackage is in bacteria-and dust-free workshop, uses the special fill of cold chain to set
Standby, fermentation liquid is filled to sterilized bottle.
Further, the shaking flask kind of step 1) is accessed seed tank amplification culture, then fermentor cultivation is received in expansion.
Further, step 1) and step 2) described in the mixing that sugar is glucose and disaccharide, it respectively accounts for 50% percent mass
Ratio.Described disaccharide is sucrose, white sugar or brown sugar.
Further, depending on product needed or technological requirement, before subpackage, also may filter that removing fungus ball.
Further, step 1) shake-flask culture based formulas: be by weight percentage: wheat bran 0.5-1.5%, Semen Maydis 1.5-
2.5%, Semen Glycines 1.5-2.5%, Herba bromi japonici 0.5-1.5%, sugar (1.5-2.5%, yeast powder 1-2%, bee pollen 0.1-0.2%, biphosphate
Potassium 0.2%, surplus is water.
Further, in step 1) and step 2, the pH value of culture medium is 5.5-7.5.
Further, described step 2) formula of culture medium is: raw materials by weight is: wheat bran 0.5-1.5%, beautiful
Rice 1.5-2.5%, Semen Glycines 1.5-2.5%, Herba bromi japonici 0.5-1.5%, sugar 1.5-2.5%, yeast powder 1-2%, bee pollen 0.1-0.2%, phosphorus
Acid dihydride potassium 0.2%, vegetable oil 0.3-0.6%, surplus is water.
Further, shaking speed 180-240r/min in step 1), shaken cultivation 7-10 at being 20-28 DEG C cultivated by shaking table
My god.
Further, step 1) and step 2) in the preparation method of culture medium be: the direct liquor of wheat bran filters, Semen Maydis, Huang
Bean, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, and the juice after filtering once adds other adjuvant, supplies water in proportion,
Load in conical flask.
Further, step 2) in, when tank temperature rise to 80 DEG C, first open air bleeding valve, discharge cold air, now air bleeding valve
It is in crack state.
The invention have the benefit that 1, change the chemosynthesis culture medium in the past fermented, but with natural plants be
Main, natural green is without chemical residual, and trace element is many, comprehensive nutrition；2, conventional formulation is only added the such monosaccharide of glucose,
The disaccharide now changing glucose and one of sucrose, white sugar, brown sugar into adds jointly, allows composition comprehensive and lasting；3, traditional zymotic is used
Defoamer processes the foam that fermentation produces, and this technology vegetable oil substitutes, the most nutritious, again without chemical residual；4, oral
Liquid is producing and in processing pouring process, omnidistance does not use high temperature, is dried, extracts, traditional or modern physics or the change such as crushes
Method, the most only effectively remains all of active component in oral liquid, and makes active component not be damaged or destroyed；
5, oral liquid changes the method in the past adding the additives such as preservative, thickening agent, stabilizer, coloring agent, from preparation shaking flask kind
To product fill all without any chemical addition agent not meeting national requirements；6, the oral liquid of this kind of method production is through authority
Detection department detection active constituent content is high；7, the method using liquid fermentation, shortens the production cycle, and yield is high, and composition is good.
Detailed description of the invention
Below in conjunction with being embodied as the present invention is further explained explanation.
Embodiment 1
1, shaking flask kind formula: raw material percentage ratio by weight is: wheat bran 0.5%, Semen Maydis 2.5%, Semen Glycines 2.5%, Herba bromi japonici 0.5%, sugar
Wherein, sugar is dextrose plus saccharose to 2.5%(, respectively accounts for 50%), yeast powder 1%, bee pollen 0.2%, potassium dihydrogen phosphate 0.2%, surplus is
Water, pH value is 5.5.
2, method: the direct liquor of wheat bran filters, Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, will filter
After juice once add other adjuvant, supply water in proportion, load in conical flask, liquid amount about 30%, by tampon (
Cotton Gossypii binds up with gauze makes) or silica gel plug fill in bottleneck, wrap laggard horizontal high voltage sterilizing with kraft paper, after emptying cold air
It is warming up to 121 DEG C, timing 35min, opens exhaust valve opening pot cover when indicator makes zero.When in bottle, culture medium temperature drops to 25 DEG C
Time, aseptically with inoculation shovel, slant strains is accessed in triangular flask, the triangular flask having connect bacterium moves into shaking table in culturing room
Cultivate, shaking speed 200r/min, shaken cultivation 8 days at 25 DEG C, when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, bacterium
Pompon is high-visible, and all burrs are obvious, when microscopy is without miscellaneous bacteria, standby.
Two, fermentor cultivation
1, culture medium: raw material percentage ratio by weight is: wheat bran 1.5%, Semen Maydis 2.5%, Semen Glycines 1.5%, Herba bromi japonici 0.5% was pulled an oar
Filter, sugar (wherein sugar is dextrose plus saccharose, respectively accounts for 50%) 1.5%, yeast powder 1%, bee pollen 0.1%, potassium dihydrogen phosphate 0.2%, plant
Thing oil 0.6%, surplus is water, and pH value is 5.5.The same Shake flask medium of preparation method.
2, liquid fermentation culturing method and condition: raw material is weighed in proportion, processing method, with shaking flask kind, stirs,
Feeding sterilizing.Liquid amount is the 60% of fermentation tank total measurement (volume).When tank temperature rise to 80 DEG C, open air bleeding valve, discharge cold air, this
Time air bleeding valve be in crack state, tank temperature rise to 121-123 DEG C, tank pressure 0.12Mpa time, timing starts, sterilizing 50min, timing
Terminating logical cooling water temperature, in tank, temperature drops to stop cooling when 30 DEG C, adjusts tank pressure 0.05Mpa, enters cultivation conditions.Will training
The shaking flask kind supported or seed tank strain are cultivated in accessing tank, inoculum concentration 10%, tank temperature 28 DEG C, tank pressure 0.04Mpa, gas
Flow 1.5m3/ h, when cultivating 36h in tank, pick test.Continuing to cultivate 6 days, when fungus ball is high-visible, all burrs are obvious, bacterium
Liquid is faint yellow, about pH5-6, residual sugar amount when being down to about 0.3%, and cultivation terminates, through checking the qualified subpackage that gets final product (during subpackage
Retain fungus ball depending on product needed or technological requirement or filter off fungus ball).
Three, subpackage, spiral cover, lamp inspection, labeling, vanning
In state specified standards purifies bacteria-and dust-free workshop, use the special filling apparatus of cold chain, fermentation liquid is filled to 30-
In the sterilized bottle of 150mL, spiral cover laggard portable lighter inspection, labeling, vanning.
Embodiment 2
1, shaking flask kind formula: raw material percentage ratio by weight is: wheat bran 1.0%, Semen Maydis 1.5%, Semen Glycines 2%, Herba bromi japonici 1.5%, sugar
Wherein, sugar is glucose and brown sugar to 1.5%(, respectively accounts for 50%), yeast powder 2%, bee pollen 0.1%, potassium dihydrogen phosphate 0.2%, surplus is
Water, pH value is 6.5.
2, method: the direct liquor of wheat bran filters, Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, will filter
After juice once add other adjuvant, supply water in proportion, load in conical flask, liquid amount about 30%, by tampon (
Cotton Gossypii binds up with gauze makes) or silica gel plug fill in bottleneck, wrap laggard horizontal high voltage sterilizing with kraft paper, after emptying cold air
It is warming up to 123 DEG C, timing 35min, opens exhaust valve opening pot cover when indicator makes zero.When in bottle, culture medium temperature drops to 28 DEG C
Time, aseptically with inoculation shovel, slant strains is accessed in triangular flask, the triangular flask having connect bacterium moves into shaking table in culturing room
Cultivate, shaking speed 200r/min, shaken cultivation 7 days at 28 DEG C, when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, bacterium
Pompon is high-visible, and all burrs are obvious, when microscopy is without miscellaneous bacteria, standby.
Two, fermentor cultivation
1, culture medium: raw material percentage ratio by weight is: wheat bran 1 %, Semen Maydis 2%, Semen Glycines 2%, Herba bromi japonici 1% making beating filtration, sugar (its
Middle sugar is dextrose plus saccharose, respectively accounts for 50%) 2%, yeast powder 1%, bee pollen 0.15%, potassium dihydrogen phosphate 0.2%, vegetable oil 0.4%,
Surplus is water, and pH value is 6.5.The same Shake flask medium of preparation method.
2, liquid fermentation culturing method and condition: raw material is weighed in proportion, processing method, with shaking flask kind, stirs,
Feeding sterilizing.Liquid amount is the 70% of fermentation tank total measurement (volume).When tank temperature rise to 80 DEG C, open air bleeding valve, discharge cold air, this
Time air bleeding valve be in crack state, when tank temperature rise to 123 DEG C, tank pressure 0.11Mpa, timing starts, sterilizing 50min, and timing terminates
Logical cooling water temperature, in tank, temperature drops to stop cooling when 30 DEG C, adjusts tank pressure 0.05Mpa, enters cultivation conditions.By cultivation well
Shaking flask kind or seed tank strain access in tank and cultivate, inoculum concentration 10%, tank temperature 28 DEG C, tank pressure 0.04Mpa, gas flow
1.5m3/ h, when cultivating 36h in tank, pick test.Continue to cultivate 7 days, when fungus ball is high-visible, week burr obvious, bacterium solution in
When faint yellow, about pH5-6, residual sugar amount are down to about 0.3%, cultivation terminates, and (regards during subpackage through the qualified subpackage that gets final product of inspection and produces
Product need or technological requirement retains fungus ball or filters off fungus ball).
Three, subpackage, spiral cover, lamp inspection, labeling, vanning
In state specified standards purifies bacteria-and dust-free workshop, use the special filling apparatus of cold chain, fermentation liquid is filled to 30-
In the sterilized bottle of 150mL, spiral cover laggard portable lighter inspection, labeling, vanning.
Embodiment 3
1, shaking flask kind formula: raw material percentage ratio by weight is: wheat bran 1.5%, Semen Maydis 2.5%, Semen Glycines 1.5%, Herba bromi japonici 1.5%, sugar
Wherein, sugar is glucose and white sugar to 2.5%(, respectively accounts for 50%), yeast powder 1%, bee pollen 0.2%, potassium dihydrogen phosphate 0.2%, surplus is
Water, pH value is 7.
2, method: the direct liquor of wheat bran filters, Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, will filter
After juice once add other adjuvant, supply water in proportion, load in conical flask, liquid amount about 30%, by tampon (
Cotton Gossypii binds up with gauze makes) or silica gel plug fill in bottleneck, wrap laggard horizontal high voltage sterilizing with kraft paper, after emptying cold air
It is warming up to 121 DEG C, timing 35min, opens exhaust valve opening pot cover when indicator makes zero.When in bottle, culture medium temperature drops to 20 DEG C
Time, aseptically with inoculation shovel, slant strains is accessed in triangular flask, the triangular flask having connect bacterium moves into shaking table in culturing room
Cultivate, shaking speed 200r/min, shaken cultivation 10 days at 20 DEG C, when bacterium solution is micro-thick, bacterium solution tint lemon yellow or tenne, bacterium
Pompon is high-visible, and all burrs are obvious, when microscopy is without miscellaneous bacteria, standby.
Two, fermentor cultivation
1, culture medium: raw material percentage ratio by weight is: wheat bran 1.5%, Semen Maydis-2.5%, Semen Glycines 1.5%, Herba bromi japonici 1.5% was pulled an oar
Filter, sugar (wherein sugar is dextrose plus saccharose, respectively accounts for 50%) 2.5%, yeast powder 1%, bee pollen 0.1%, potassium dihydrogen phosphate 0.2%, plant
Thing oil 0.6%, surplus is water, and pH value is 5.5.The same Shake flask medium of preparation method.
2, liquid fermentation culturing method and condition: raw material is weighed in proportion, processing method, with shaking flask kind, stirs,
Feeding sterilizing.Liquid amount is the 55% of fermentation tank total measurement (volume).When tank temperature rise to 80 DEG C, open air bleeding valve, discharge cold air, this
Time air bleeding valve be in crack state, tank temperature rise to 121-123 DEG C, tank pressure 0.13Mpa time, timing starts, sterilizing 50min, timing
Terminating logical cooling water temperature, in tank, temperature drops to stop cooling when 30 DEG C, adjusts tank pressure 0.05Mpa, enters cultivation conditions.Will training
The shaking flask kind supported or seed tank strain are cultivated in accessing tank, inoculum concentration 10%, tank temperature 28 DEG C, tank pressure 0.05Mpa, gas
Flow 1.5m3/ h, when cultivating 36h in tank, pick test.Continuing to cultivate 5 days, when fungus ball is high-visible, all burrs are obvious, bacterium
Liquid is faint yellow, about pH5-6, residual sugar amount when being down to about 0.3%, and cultivation terminates, through checking the qualified subpackage that gets final product (during subpackage
Retain fungus ball depending on product needed or technological requirement or filter off fungus ball).
Three, subpackage, spiral cover, lamp inspection, labeling, vanning
In state specified standards purifies bacteria-and dust-free workshop, use the special filling apparatus of cold chain, fermentation liquid is filled to 30-
In the sterilized bottle of 150mL, spiral cover laggard portable lighter inspection, labeling, vanning.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope of present disclosure, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (10)
1. the preparation method of a hedgehog hydnum oral liquid, it is characterised in that step is as follows:
1) shaking flask kind is prepared:
Culture medium is loaded in conical flask, liquid amount 25-35%, after sealing at a temperature of 121-123 DEG C sterilizing 35 minutes,
When culture medium temperature drops to 20-28 DEG C, in slant strains aseptically accesses triangular flask, upper shaking table is cultivated, and works as bacterium solution
Micro-thick, bacterium solution tint lemon yellow or tenne, mycelium pellet is high-visible, and all burrs are obvious, when microscopy is without miscellaneous bacteria, standby；
2) fermentor cultivation:
Culture medium being loaded in fermentation tank, liquid amount is the 55-75% of fermentation tank total capacity, when tank temperature rise is to 121-123 DEG C, tank
During pressure 0.11-0.13Mpa, sterilizing 50min, then lead to cooling water temperature, in tank, temperature drops to stop when 30 DEG C, adjusts tank pressure
To 0.05MPa, enter cultivation conditions；
Cultivate in cultured shaking flask kind strain is accessed tank, inoculum concentration 5-15%, tank temperature 20-28 DEG C, tank pressure 0.04-
0.05MPa, gas flow 1.5m3/ h, when cultivating 36-48h in tank, pick test；
Continuing to cultivate 5-7 days, when fungus ball is high-visible, all burrs are obvious, and bacterium solution is faint yellow, about pH5-6, residual sugar amount fall
During to about 0.3%, cultivation terminates, and carries out subpackage.
The preparation method of hedgehog hydnum oral liquid the most according to claim 1, it is characterised in that the shaking flask kind of step 1) is accessed
Seed tank amplification culture, then expand receive fermentor cultivation.
The preparation method of hedgehog hydnum oral liquid the most according to claim 1, it is characterised in that be filtered to remove fungus ball before subpackage.
4. shaking flask kind special culture media in the preparation method of a hedgehog hydnum oral liquid, it is characterised in that be by weight percentage:
Wheat bran 0.5-1.5%, Semen Maydis 1.5-2.5%, Semen Glycines 1.5-2.5%, Herba bromi japonici 0.5-1.5%, sugar 1.5-2.5%, yeast powder 1-2%, honeybee
Pollen 0.1-0.2%, potassium dihydrogen phosphate 0.2%, surplus is water.
5. fermentor cultivation special culture media in the preparation method of a hedgehog hydnum oral liquid, it is characterised in that raw material by weight hundred
Proportion by subtraction is: wheat bran 0.5-1.5%, Semen Maydis 1.5-2.5%, Semen Glycines 1.5-2.5%, Herba bromi japonici 0.5-1.5%, sugar 1.5-2.5%, yeast powder
1-2%, bee pollen 0.1-0.2%, potassium dihydrogen phosphate 0.2%, vegetable oil 0.3-0.6%, surplus is water.
6. according to the preparation method of the hedgehog hydnum oral liquid described in claim 4 or 5, it is characterised in that described sugar is glucose
With the mixing of disaccharide, it respectively accounts for 50% mass percent, and described disaccharide is sucrose, white sugar or brown sugar.
7. according to the preparation method of the hedgehog hydnum oral liquid described in claim 4 or 5, it is characterised in that the pH value of culture medium is
5.5-7.5。
The preparation method of hedgehog hydnum oral liquid the most according to claim 1, it is characterised in that shaking speed 180-in step 1)
240r/min, shaken cultivation 7-10 days at being 20-28 DEG C cultivated by shaking table.
9. according to the preparation method of the hedgehog hydnum oral liquid described in claim 5 or 7, it is characterised in that step 1) and step 2) in
The preparation method of culture medium is: the direct liquor of wheat bran filters, and Semen Maydis, Semen Glycines, Herba bromi japonici are impregnated with without filter and remove residue of pulling an oar after hard-core, will
Juice after filtration once adds other adjuvant, supplies water in proportion, loads in conical flask.
The preparation method of hedgehog hydnum oral liquid the most according to claim 1, it is characterised in that step 2) in, when tank temperature rise extremely
When 80 DEG C, first opening air bleeding valve, discharge cold air, now air bleeding valve is in crack state.
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