CN106071270A - A kind of dog grain improving immunity and preparation method thereof - Google Patents
A kind of dog grain improving immunity and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of dog grain improving immunity and preparation method thereof, Chinese medicine extract in raw material uses particular extraction procedure to propose effective ingredient, add natural plant composition again, part material is carried out pretreatment, the raw material of pretreatment can strengthen the effect of Chinese medicine extract and natural plant composition, it is healthy that pet dog not only nursed one's health from root by the dog grain prepared, enhancing body nonspecific immunity, it is effectively improved pet dog resistance, but also pet dog the intestines and stomach can be regulated, acute gastritis is had certain therapeutical effect.
Description
Technical field
The present invention relates to a kind of dog grain improving immunity and preparation method thereof, belong to field of animal feed.
Background technology
Feedstuff, is the general name of the food of everyone domesticated animal, compares narrowly general feeds and generally refers to agricultural
Or the food of animal husbandry domesticated animal.Feedstuff include Semen sojae atricolor, bean cake, Semen Maydis, fish flour, aminoacid, the miscellaneous dregs of rice, additive, whey powder,
The feedstuff of more than ten kinds such as oils and fats, meat meal tankage, corn, sugar grass.
Animal feed is all directly to add various Chinese medicines, DeGrain at present.
Summary of the invention
It is an object of the invention to provide a kind of dog grain improving immunity and preparation method thereof, the Chinese medicine extract in raw material
Use particular extraction procedure to propose effective ingredient, then add natural plant composition, part material is carried out pretreatment, pretreatment
Raw material can strengthen the effect of Chinese medicine extract and natural plant composition, and prepared dog grain is not only nursed one's health pet dog from root and is good for
Health, enhancing body nonspecific immunity, it is effectively improved pet dog resistance, but also pet dog the intestines and stomach can be regulated, to acute gastritis
There is certain therapeutical effect.
A kind of dog grain improving immunity, is made up of the raw material of following masses number: Chinese medicine extract 900-1000 part, yellow
Stilbene Carnis Gallus domesticus 90-110 part, Os Gallus domesticus 70-80 part, egg powder 70-80 part, Carnis Sus domestica 70-80 part, beef 70-80 part, Radix Astragali mushroom 65-
75 parts, chicken oil 10-15 part, Adeps Bovis seu Bubali 10-15 part, soybean oil 10-15 part, Semen Maydis oil 10-15 part, rice 280-320 part, gluten meal
120-130 part, Radix Dauci Sativae 20-30 part, Fructus Cucurbitae moschatae 20-30 part, Fructus Lycopersici esculenti 20-30 part, Citrus meat 20-30 part, vitamin 8-12 part,
Mineral 8-12 part, zeolite powder 8-12 part, TYR 5-10 part, DL-methionine 5-10 part, 1B 5-10 part, cattle sulphur
Acid 5-10 part, potassium sorbate 5-10 part, antioxidant 5-10 part.
Described Chinese medicine extract is made up of the raw material of following masses number: Radix Astragali 35-45 part, Flos Chrysanthemi 23-27 part, Fructificatio Amaurodermatis Rudae
Fiber crops 20-25 part.
Described vitamin is vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B one or more.
Described mineral be sodium chloride, copper sulfate, ferrous sulfate one or more.
Described antioxidant is one or both of BHA, BHT.
The preparation method of described Chinese medicine extract is:
(1) Radix Astragali is pulverized, at room temperature soak 18-22 hour with the Ether-Acetone mixed liquor of 17-19 times of quality, mistake
Filter, obtains filtrate I and filtering residue I；
(2) Flos Chrysanthemi is pulverized, add 95% ethanol of 21-23 times of quality, add filtering residue I, 70-80 DEG C of heating and refluxing extraction
140-160 minute, filter, obtain filtrate II；
(3) 1100 macroporous adsorptive resins on filtrate II, upper column flow rate is 1.5-1.7BV/h, successively with the 80% of 3BV
Ethanol, 60% ethanol of 2BV, 40% ethanol of 1BV, 20% ethanol of 0.5BV, carry out gradient elution, and elution flow rate is 1.5-
1.7BV/h, collects gradient eluent；
(4) gradient eluent decompression and solvent recovery to without alcohol taste, extract I is obtained；
(5) Semen Sesami Nigrum is pulverized, add to filtrate I, supersound extraction 40-50 minute, filter, obtain filtrate II and filtering residue
Ⅱ；
(6) filtrate II concentrating under reduced pressure is volatilized solvent, obtain extract II；
(7) extract I, extract II and filtering residue II mix homogeneously, it is dried, is Chinese medicine extract.
Wherein, in Ether-Acetone mixed liquor, ether and acetone volume ratio are 1:4
Wherein, in step (3), the volume ratio of filtrate II and 1100 macroporous adsorbent resins is 1.5:1, the height of resin column and
Diameter is than for 7:1.
The preparation method of described Radix Astragali Carnis Gallus domesticus is: Fresh chicken is shredded, and adds the Radix Astragali and water, Fresh chicken, the Radix Astragali and water
Mass ratio be 1:0.5-0.7:4.5-5.5, decoct 3-4 hour, filter, remaining filtering residue is Radix Astragali Carnis Gallus domesticus.
The preparation method of described Radix Astragali mushroom is: mushroom shredding, adds the Radix Astragali and water, the matter of mushroom, the Radix Astragali and water
Amount ratio is 1:0.4-0.6:2.5-3.5, decocts 3-4 hour, filters, and remaining filtering residue is Radix Astragali mushroom.
Wherein mushroom be Lentinus Edodes, agrocyb eaegerita one or both.
A kind of dog grain improving immunity, its preparation method is:
(1) rice and gluten meal mixer are mixed 10 minutes, after micronizing, standby；
(2) Chinese medicine extract, the Radix Astragali Carnis Gallus domesticus of pulverizing, the Os Gallus domesticus of pulverizing, the egg powder of pulverizing, the Carnis Sus domestica of pulverizing, powder
Broken beef, the Radix Astragali mushroom of pulverizing, the Radix Dauci Sativae of pulverizing, the Fructus Cucurbitae moschatae of pulverizing, the Fructus Lycopersici esculenti of pulverizing, the Citrus meat of pulverizing, dimension are raw
Element, mineral, zeolite powder, TYR, DL-methionine, 1B, taurine, potassium sorbate, antioxidant and upper step
The material obtained mixes 10 minutes；
(3) mixed material being carried out expanded, 100-120 DEG C of baking oven of expanded rear entrance is dried；
(4) taking chicken oil, Adeps Bovis seu Bubali, soybean oil, Semen Maydis oil is put into spout pouring pot, dried material is carried out oil spout technique；
(5) material of after end of injection cools down, packaging, to obtain final product.
The solution have the advantages that:
1, the Radix Astragali, Flos Chrysanthemi and Semen Sesami Nigrum use specific preparation method to obtain Chinese medicine extract, have effectively extracted raising
The composition of immunity, effect is better than being provided without extracting method and the Radix Astragali of other extracting method, Flos Chrysanthemi and Semen Sesami Nigrum.
2, raw material uses the Radix Dauci Sativae of plant component, Fructus Cucurbitae moschatae, Fructus Lycopersici esculenti and Citrus meat, containing natural constituent, without secondary
Effect.
3, preparation process first carries out pretreatment with the Radix Astragali Carnis Gallus domesticus and Radix Astragali mushroom respectively, then make with other raw materials and carry
The dog grain of high immunity.
4, the raw material of the pretreatment of the present invention can strengthen the effect of Chinese medicine extract and natural plant composition, San Zhelian
With, it is healthy that pet dog not only nursed one's health from root by the dog grain prepared, and enhancing body nonspecific immunity is effectively improved pet dog opposing
Power, but also pet dog the intestines and stomach can be regulated, acute gastritis is had certain therapeutical effect.
Detailed description of the invention
Below by embodiment, the invention will be further described.
Embodiment 1:
A kind of dog grain improving immunity, is made up of the raw material of following masses number: Chinese medicine extract 900 parts, Radix Astragali chicken
Meat 90 parts, Os Gallus domesticus 70 parts, egg powder 70 parts, Carnis Sus domestica 70 parts, 70 parts of beef, Radix Astragali mushroom 65 parts, chicken oil 10 parts, 10 parts of Adeps Bovis seu Bubali,
Soybean oil 10 parts, Semen Maydis oil 10 parts, 280 parts of rice, gluten meal 120 parts, 20 parts of Radix Dauci Sativae, 20 parts of Fructus Cucurbitae moschatae, Fructus Lycopersici esculenti 20 parts, Citrus chachiensis Hort.
Fructus Citri tangerinae meat 20 parts, vitamin 8 parts, mineral 8 parts, zeolite powder 8 parts, TYR 5 parts, DL-methionine 5 parts, 1B 5 parts,
Taurine 5 parts, potassium sorbate 5 parts, antioxidant 5 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: the Radix Astragali 35 parts, Flos Chrysanthemi 23 parts, Semen Sesami Nigrum 20
Part.
Described vitamin is vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B one or more.
Described mineral be sodium chloride, copper sulfate, ferrous sulfate one or more.
Described antioxidant is one or both of BHA, BHT.
The preparation method of described Chinese medicine extract is:
(1) Radix Astragali is pulverized, at room temperature soaks 18-22 hour with the Ether-Acetone mixed liquor of 17 times of quality, filter,
Obtain filtrate I and filtering residue I；
(2) Flos Chrysanthemi is pulverized, add 95% ethanol of 21 times of quality, add filtering residue I, 70 DEG C of heating and refluxing extraction 140 points
Clock, filters, obtains filtrate II；
(3) 1100 macroporous adsorptive resins on filtrate II, upper column flow rate is 1.5BV/h, successively with 80% second of 3BV
Alcohol, 60% ethanol of 2BV, 40% ethanol of 1BV, 20% ethanol of 0.5BV, carry out gradient elution, and elution flow rate is 1.5BV/
H, collects gradient eluent；
(4) gradient eluent decompression and solvent recovery to without alcohol taste, extract I is obtained；
(5) Semen Sesami Nigrum is pulverized, add to filtrate I, supersound extraction 40 minutes, filter, obtain filtrate II and filtering residue II；
(6) filtrate II concentrating under reduced pressure is volatilized solvent, obtain extract II；
(7) extract I, extract II and filtering residue II mix homogeneously, it is dried, is Chinese medicine extract.
Wherein, in Ether-Acetone mixed liquor, ether and acetone volume ratio are 1:4.
Wherein, in step (3), the volume ratio of filtrate II and 1100 macroporous adsorbent resins is 1.5:1, the height of resin column and
Diameter is than for 7:1.
The preparation method of described Radix Astragali Carnis Gallus domesticus is: Fresh chicken is shredded, and adds the Radix Astragali and water, Fresh chicken, the Radix Astragali and water
Mass ratio be 1:0.5:4.5, decoct 3 hours, filter, remaining filtering residue is Radix Astragali Carnis Gallus domesticus.
The preparation method of described Radix Astragali mushroom is: mushroom shredding, adds the Radix Astragali and water, the matter of mushroom, the Radix Astragali and water
Amount ratio is 1:0.4:2.5, decocts 3-4 hour, filters, and remaining filtering residue is Radix Astragali mushroom.
Wherein mushroom be Lentinus Edodes, agrocyb eaegerita one or both.
A kind of dog grain improving immunity, its preparation method is:
(1) rice and gluten meal mixer are mixed 10 minutes, after micronizing, standby；
(2) Chinese medicine extract, the Radix Astragali Carnis Gallus domesticus of pulverizing, the Os Gallus domesticus of pulverizing, the egg powder of pulverizing, the Carnis Sus domestica of pulverizing, powder
Broken beef, the Radix Astragali mushroom of pulverizing, the Radix Dauci Sativae of pulverizing, the Fructus Cucurbitae moschatae of pulverizing, the Fructus Lycopersici esculenti of pulverizing, the Citrus meat of pulverizing, dimension are raw
Element, mineral, zeolite powder, TYR, DL-methionine, 1B, taurine, potassium sorbate, antioxidant and upper step
The material obtained mixes 10 minutes；
(3) mixed material being carried out expanded, 100 DEG C of baking ovens of expanded rear entrance are dried；
(4) taking chicken oil, Adeps Bovis seu Bubali, soybean oil, Semen Maydis oil is put into spout pouring pot, dried material is carried out oil spout technique；
(5) material of after end of injection cools down, packaging, to obtain final product.
Embodiment 2:
A kind of dog grain improving immunity, is made up of the raw material of following masses number: Chinese medicine extract 950 parts, Radix Astragali chicken
Meat 100 parts, Os Gallus domesticus 75 parts, egg powder 75 parts, Carnis Sus domestica 75 parts, 75 parts of beef, Radix Astragali mushroom 70 parts, chicken oil 12 parts, 12 parts of Adeps Bovis seu Bubali,
Soybean oil 12 parts, Semen Maydis oil 12 parts, 300 parts of rice, gluten meal 125 parts, 25 parts of Radix Dauci Sativae, 25 parts of Fructus Cucurbitae moschatae, Fructus Lycopersici esculenti 25 parts, Citrus chachiensis Hort.
Fructus Citri tangerinae meat 25 parts, vitamin 10 parts, mineral 10 parts, zeolite powder 10 parts, TYR 7 parts, DL-methionine 7 parts, 1B 7
Part, taurine 7 parts, potassium sorbate 7 parts, antioxidant 7 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: the Radix Astragali 40 parts, Flos Chrysanthemi 25 parts, Semen Sesami Nigrum 22
Part.
Described vitamin is vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B one or more.
Described mineral be sodium chloride, copper sulfate, ferrous sulfate one or more.
Described antioxidant is one or both of BHA, BHT.
The preparation method of described Chinese medicine extract is:
(1) Radix Astragali is pulverized, at room temperature soak 20 hours with the Ether-Acetone mixed liquor of 18 times of quality, filter, obtain filter
Liquid I and filtering residue I；
(2) Flos Chrysanthemi is pulverized, add 95% ethanol of 22 times of quality, add filtering residue I, 75 DEG C of heating and refluxing extraction 150 points
Clock, filters, obtains filtrate II；
(3) 1100 macroporous adsorptive resins on filtrate II, upper column flow rate is 1.6BV/h, successively with 80% second of 3BV
Alcohol, 60% ethanol of 2BV, 40% ethanol of 1BV, 20% ethanol of 0.5BV, carry out gradient elution, and elution flow rate is 1.6BV/
H, collects gradient eluent；
(4) gradient eluent decompression and solvent recovery to without alcohol taste, extract I is obtained；
(5) Semen Sesami Nigrum is pulverized, add to filtrate I, supersound extraction 45 minutes, filter, obtain filtrate II and filtering residue II；
(6) filtrate II concentrating under reduced pressure is volatilized solvent, obtain extract II；
(7) extract I, extract II and filtering residue II mix homogeneously, it is dried, is Chinese medicine extract.
Wherein, in Ether-Acetone mixed liquor, ether and acetone volume ratio are 1:4.
Wherein, in step (3), the volume ratio of filtrate II and 1100 macroporous adsorbent resins is 1.5:1, the height of resin column and
Diameter is than for 7:1.
The preparation method of described Radix Astragali Carnis Gallus domesticus is: Fresh chicken is shredded, and adds the Radix Astragali and water, Fresh chicken, the Radix Astragali and water
Mass ratio be 1:0.6:5, decoct 3.5 hours, filter, remaining filtering residue is Radix Astragali Carnis Gallus domesticus.
The preparation method of described Radix Astragali mushroom is: mushroom shredding, adds the Radix Astragali and water, the matter of mushroom, the Radix Astragali and water
Amount ratio is 1:0.5:3, decocts 3.5 hours, filters, and remaining filtering residue is Radix Astragali mushroom.
Wherein mushroom be Lentinus Edodes, agrocyb eaegerita one or both.
A kind of dog grain improving immunity, its preparation method is:
(1) rice and gluten meal mixer are mixed 10 minutes, after micronizing, standby；
(2) Chinese medicine extract, the Radix Astragali Carnis Gallus domesticus of pulverizing, the Os Gallus domesticus of pulverizing, the egg powder of pulverizing, the Carnis Sus domestica of pulverizing, powder
Broken beef, the Radix Astragali mushroom of pulverizing, the Radix Dauci Sativae of pulverizing, the Fructus Cucurbitae moschatae of pulverizing, the Fructus Lycopersici esculenti of pulverizing, the Citrus meat of pulverizing, dimension are raw
Element, mineral, zeolite powder, TYR, DL-methionine, 1B, taurine, potassium sorbate, antioxidant and upper step
The material obtained mixes 10 minutes；
(3) mixed material being carried out expanded, 110 DEG C of baking ovens of expanded rear entrance are dried；
(4) taking chicken oil, Adeps Bovis seu Bubali, soybean oil, Semen Maydis oil is put into spout pouring pot, dried material is carried out oil spout technique；
(5) material of after end of injection cools down, packaging, to obtain final product.
Embodiment 3:
A kind of dog grain improving immunity, is made up of the raw material of following masses number: Chinese medicine extract 1000 parts, Radix Astragali chicken
Meat 110 parts, Os Gallus domesticus 80 parts, egg powder 80 parts, Carnis Sus domestica 80 parts, 80 parts of beef, Radix Astragali mushroom 75 parts, chicken oil 15 parts, 15 parts of Adeps Bovis seu Bubali,
Soybean oil 15 parts, Semen Maydis oil 15 parts, 320 parts of rice, gluten meal 130 parts, 30 parts of Radix Dauci Sativae, 30 parts of Fructus Cucurbitae moschatae, Fructus Lycopersici esculenti 30 parts, Citrus chachiensis Hort.
Fructus Citri tangerinae meat 30 parts, vitamin B12 part, mineral 12 parts, zeolite powder 12 parts, TYR 10 parts, DL-methionine 10 parts, 1B
10 parts, taurine 10 parts, potassium sorbate 10 parts, antioxidant 10 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: the Radix Astragali 45 parts, Flos Chrysanthemi 27 parts, Semen Sesami Nigrum 25
Part.
Described vitamin is vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B one or more.
Described mineral be sodium chloride, copper sulfate, ferrous sulfate one or more.
Described antioxidant is one or both of BHA, BHT.
The preparation method of described Chinese medicine extract is:
(1) Radix Astragali is pulverized, at room temperature soak 22 hours with the Ether-Acetone mixed liquor of 19 times of quality, filter, obtain filter
Liquid I and filtering residue I；
(2) Flos Chrysanthemi is pulverized, add 95% ethanol of 23 times of quality, add filtering residue I, 80 DEG C of heating and refluxing extraction 160 points
Clock, filters, obtains filtrate II；
(3) 1100 macroporous adsorptive resins on filtrate II, upper column flow rate is 1.7BV/h, successively with 80% second of 3BV
Alcohol, 60% ethanol of 2BV, 40% ethanol of 1BV, 20% ethanol of 0.5BV, carry out gradient elution, and elution flow rate is 1.7BV/
H, collects gradient eluent；
(4) gradient eluent decompression and solvent recovery to without alcohol taste, extract I is obtained；
(5) Semen Sesami Nigrum is pulverized, add to filtrate I, supersound extraction 50 minutes, filter, obtain filtrate II and filtering residue II；
(6) filtrate II concentrating under reduced pressure is volatilized solvent, obtain extract II；
(7) extract I, extract II and filtering residue II mix homogeneously, it is dried, is Chinese medicine extract.
Wherein, in Ether-Acetone mixed liquor, ether and acetone volume ratio are 1:4.
Wherein, in step (3), the volume ratio of filtrate II and 1100 macroporous adsorbent resins is 1.5:1, the height of resin column and
Diameter is than for 7:1.
The preparation method of described Radix Astragali Carnis Gallus domesticus is: Fresh chicken is shredded, and adds the Radix Astragali and water, Fresh chicken, the Radix Astragali and water
Mass ratio be 1:0.7:5.5, decoct 4 hours, filter, remaining filtering residue is Radix Astragali Carnis Gallus domesticus.
The preparation method of described Radix Astragali mushroom is: mushroom shredding, adds the Radix Astragali and water, the matter of mushroom, the Radix Astragali and water
Amount ratio is 1:0.6:3.5, decocts 4 hours, filters, and remaining filtering residue is Radix Astragali mushroom.
Wherein mushroom be Lentinus Edodes, agrocyb eaegerita one or both.
A kind of dog grain improving immunity, its preparation method is:
(1) rice and gluten meal mixer are mixed 10 minutes, after micronizing, standby；
(2) Chinese medicine extract, the Radix Astragali Carnis Gallus domesticus of pulverizing, the Os Gallus domesticus of pulverizing, the egg powder of pulverizing, the Carnis Sus domestica of pulverizing, powder
Broken beef, the Radix Astragali mushroom of pulverizing, the Radix Dauci Sativae of pulverizing, the Fructus Cucurbitae moschatae of pulverizing, the Fructus Lycopersici esculenti of pulverizing, the Citrus meat of pulverizing, dimension are raw
Element, mineral, zeolite powder, TYR, DL-methionine, 1B, taurine, potassium sorbate, antioxidant and upper step
The material obtained mixes 10 minutes；
(3) mixed material being carried out expanded, 120 DEG C of baking ovens of expanded rear entrance are dried；
(4) taking chicken oil, Adeps Bovis seu Bubali, soybean oil, Semen Maydis oil is put into spout pouring pot, dried material is carried out oil spout technique；
(5) material of after end of injection cools down, packaging, to obtain final product.
Comparative example 1:
A kind of dog grain improving immunity, is made up of the raw material of following masses number: Chinese medicine extract 1200 parts, Radix Astragali chicken
Meat 80 parts, Os Gallus domesticus 90 parts, egg powder 60 parts, Carnis Sus domestica 90 parts, 60 parts of beef, Radix Astragali mushroom 80 parts, chicken oil 7 parts, 20 parts of Adeps Bovis seu Bubali, greatly
7 parts of Oleum Glycines, Semen Maydis oil 20 parts, 250 parts of rice, gluten meal 150 parts, 15 parts of Radix Dauci Sativae, 35 parts of Fructus Cucurbitae moschatae, Fructus Lycopersici esculenti 15 parts, Citrus meat
35 parts, vitamin 5 parts, mineral 15 parts, zeolite powder 5 parts, TYR 12 parts, DL-methionine 3 parts, 1B 15 parts, cattle
Sulfonic acid 3 parts, potassium sorbate 15 parts, antioxidant 3 parts.
Described Chinese medicine extract is made up of the raw material of following masses number: the Radix Astragali 30 parts, Flos Chrysanthemi 30 parts, Semen Sesami Nigrum 17
Part.
Remaining is with embodiment 1.
Comparative example 2:
A kind of dog grain improving immunity, the preparation method of described Chinese medicine extract is:
(1) Radix Astragali is pulverized, at room temperature soaks 20 hours with 70% ethanol-acetone mixed liquor of 18 times of quality, filter,
Obtain filtrate I and filtering residue I；
(2) Flos Chrysanthemi is pulverized, add 50% ethanol of 22 times of quality, add filtering residue I, 75 DEG C of heating and refluxing extraction 150 points
Clock, filters, obtains filtrate II；
(3) D101 macroporous adsorptive resins on filtrate II, upper column flow rate is 1.6BV/h, successively with 90% second of 3BV
Alcohol, 70% ethanol of 2BV, 50% ethanol of 1BV, 30% ethanol of 0.5BV, carry out gradient elution, and elution flow rate is 1.5-
1.7BV/h, collects gradient eluent；
(4) gradient eluent decompression and solvent recovery to without alcohol taste, extract I is obtained；
(5) Semen Sesami Nigrum is pulverized, add to filtrate I, supersound extraction 45 minutes, filter, obtain filtrate II and filtering residue II；
(6) filtrate II concentrating under reduced pressure is volatilized solvent, obtain extract II；
(7) extract I, extract II and filtering residue II mix homogeneously, it is dried, is Chinese medicine extract.
Wherein, in 70% ethanol-acetone mixed liquor, 70% ethanol and acetone volume ratio are 1:4
Wherein, in step (3), the volume ratio of filtrate II and D101 macroporous adsorbent resin is 1.5:1, the height of resin column and
Diameter is than for 7:1.
Remaining is with embodiment 2.
Comparative example 3:
A kind of dog grain improving immunity, is made up of the raw material of following masses number: Chinese medicine extract 1000 parts, Os Gallus domesticus 80
Part, egg powder 80 parts, Carnis Sus domestica 80 parts, 80 parts of beef, chicken oil 15 parts, 15 parts of Adeps Bovis seu Bubali, soybean oil 15 parts, Semen Maydis oil 15 parts, rice
320 parts, gluten meal 130 parts, 30 parts of Radix Dauci Sativae, 30 parts of Fructus Cucurbitae moschatae, Fructus Lycopersici esculenti 30 parts, Citrus meat 30 parts, vitamin B12 part, mineral
12 parts, zeolite powder 12 parts, TYR 10 parts, DL-methionine 10 parts, 1B 10 parts, taurine 10 parts, potassium sorbate 10
Part, antioxidant 10 parts.
A kind of dog grain improving immunity, its preparation method is:
(1) rice and gluten meal mixer are mixed 10 minutes, after micronizing, standby；
(2) Chinese medicine extract, the Os Gallus domesticus of pulverizing, the egg powder of pulverizing, the Carnis Sus domestica of pulverizing, the beef of pulverizing, pulverizing
Radix Dauci Sativae, the Fructus Cucurbitae moschatae of pulverizing, the Fructus Lycopersici esculenti of pulverizing, the Citrus meat of pulverizing, vitamin, mineral, zeolite powder, TYR, DL-
The material that methionine, 1B, taurine, potassium sorbate, antioxidant obtain with upper step mixes 10 minutes；
(3) mixed material being carried out expanded, 120 DEG C of baking ovens of expanded rear entrance are dried；
(4) taking chicken oil, Adeps Bovis seu Bubali, soybean oil, Semen Maydis oil is put into spout pouring pot, dried material is carried out oil spout technique；
(5) material of after end of injection cools down, packaging, to obtain final product.
Remaining is with embodiment 3.
1. the present invention effect to improving immunity:
Taking 70 mices, body weight 18-24g, be randomly divided into 7 groups, often group 10, takes 6 groups, respectively by embodiment 1-3 and right
The dog grain that ratio 1-3 feeding embodiment 1-3 and comparative example 1-3 group prepare, 1.5g/kg mice every day, 1 group of matched group gives equivalent
Distilled water, once a day, gives 30 days continuously, and in the 25th day lumbar injection 2%SRBC, after immune 5 days, dislocation is put to death, and takes spleen,
200 eye mesh screens filter, and wash 3 times with Hanks liquid, and each 100Or/min is centrifuged 10min, are trained in 5mlRPM1640 by cell suspension
In nutrient solution.By top layer culture medium heating for dissolving, 45 DEG C of water bath heat preservations, mix with 2 times of concentration Hanks liquid of equivalent, subpackage small test tube,
Often manage O.5ml, add 50 μ l110%SRBC, 20 μ l splenocyte suspensions, mixing, reviewing, CO2 gas incubator is cultivated 1 little
Time, adding the complement (1:10) with the dilution of SA buffer, continue to cultivate 1 hour, count hemolysis plaque number, result is as shown in table 1.
Table 1 present invention on the impact of mouse antibodies cellulation (N=10)
As known from Table 1, the dog grain (embodiment 1-3) that the present invention prepares can with the antibody-producting cell of significantly raised mice,
And effect is substantially better than comparative example 1 (proportioning raw materials is different) and comparative example 2 (extraction process of Chinese medicine extract is different), contrast
Example 3 (being not added with Radix Astragali mushroom and Radix Astragali Carnis Gallus domesticus in raw material), especially optimum with the effect of embodiment 2, it is seen that proportioning raw materials, in
The curative effect of prepared product is all had an impact by the extraction process of medicament extract and material composition, therefore present invention employs specific
Proportioning raw materials, the extraction process of Chinese medicine extract and select suitable material composition, it is thin that prepared dog grain is improved antibody tormation
The effect of born of the same parents.
2. the present invention effect to acute gastritis
Take SD rat 70, body weight (200g ± 120g), male and female half and half, be randomly divided into 7 groups, often group 10, be respectively sky
White normal saline group, embodiment 1-3 group and comparative example 1-3 group, the dog grain prepared by embodiment 1-3 and comparative example 1-3 group respectively
Being administered 1.5g/kg, blank normal saline group gives reason saline 1mL/100g.Before experiment, water 48h is can't help in fasting, after being administered 1h, and every
Rats gavaged 1.5mL hydrochloric acid-ethanol (the hydrochloric 1.7mL of every 100mL and dehydrated alcohol 60mL), after 1h, cervical dislocation is put to death big
Mus, cuts off along greater gastric curvature, tiling, observes gastric mucosa.
Ulcer index computational methods: gastric mucosa contrafluxion is 1 point, petechial hemorrhage or erosion are respectively 1 point, wire erosion 1
Individual meter 3 points.Ulcer index suppression ratio=(blank group ulcer index-medication group ulcer index)/blank group ulcer refers to
Number × 100.Result is as shown in table 1.
Table 1 present invention on the impact of acute gastric mucosal lesion (N=10)
As known from Table 2, the dog grain (embodiment 1-3) that the present invention prepares can be substantially reduced the ulcer index of rat, and
Effect is substantially better than comparative example 1 (proportioning raw materials is different) and comparative example 2 (extraction process of Chinese medicine extract is different), comparative example 3
(being not added with Radix Astragali mushroom and Radix Astragali Carnis Gallus domesticus in raw material), the especially effect with embodiment 2 are optimum, it is seen that proportioning raw materials, Chinese medicine
The curative effect of prepared product is all had an impact by the extraction process of extract and material composition, therefore present invention employs the most former
Expecting proportioning, the extraction process of Chinese medicine extract and select suitable material composition, acute gastritis is had certain by prepared dog grain
Therapeutical effect.
Claims (9)
1. improve a dog grain for immunity, it is characterized by: be made up of the raw material of following masses number: Chinese medicine extract 900-
1000 parts, Radix Astragali Carnis Gallus domesticus 90-110 part, Os Gallus domesticus 70-80 part, egg powder 70-80 part, Carnis Sus domestica 70-80 part, beef 70-80 part is yellow
Stilbene mushroom 65-75 part, chicken oil 10-15 part, Adeps Bovis seu Bubali 10-15 part, soybean oil 10-15 part, Semen Maydis oil 10-15 part, rice 280-320
Part, gluten meal 120-130 part, Radix Dauci Sativae 20-30 part, Fructus Cucurbitae moschatae 20-30 part, Fructus Lycopersici esculenti 20-30 part, Citrus meat 20-30 part, dimension is raw
Element 8-12 part, mineral 8-12 part, zeolite powder 8-12 part, TYR 5-10 part, DL-methionine 5-10 part, 1B 5-
10 parts, taurine 5-10 part, potassium sorbate 5-10 part, antioxidant 5-10 part.
A kind of dog grain improving immunity the most as claimed in claim 1, is characterized by: Chinese medicine extract is by following masses number
Raw material make: Radix Astragali 35-45 part, Flos Chrysanthemi 23-27 part, Semen Sesami Nigrum 20-25 part.
A kind of dog grain improving immunity the most as claimed in claim 1, is characterized by: vitamin is vitamin A, vitamin D3、
Vitamin E, vitamin K3, vitamin B one or more.
A kind of dog grain improving immunity the most as claimed in claim 1, is characterized by: mineral is sodium chloride, copper sulfate, sulfur
One or more of acid ferrous iron.
A kind of dog grain improving immunity the most as claimed in claim 1, is characterized by: antioxidant is the one of BHA, BHT
Or two kinds.
A kind of dog grain improving immunity the most as claimed in claim 1 or 2, is characterized by: the preparation method of Chinese medicine extract
For:
(1) Radix Astragali is pulverized, at room temperature soak 18-22 hour with the Ether-Acetone mixed liquor of 17-19 times of quality, filter,
Filtrate I and filtering residue I；
(2) Flos Chrysanthemi is pulverized, add 95% ethanol of 21-23 times of quality, add filtering residue I, 70-80 DEG C of heating and refluxing extraction 140-
160 minutes, filter, obtain filtrate II；
(3) 1100 macroporous adsorptive resins on filtrate II, upper column flow rate is 1.5-1.7BV/h, successively with 80% second of 3BV
Alcohol, 60% ethanol of 2BV, 40% ethanol of 1BV, 20% ethanol of 0.5BV, carry out gradient elution, and elution flow rate is 1.5-
1.7BV/h, collects gradient eluent；
(4) gradient eluent decompression and solvent recovery to without alcohol taste, extract I is obtained；
(5) Semen Sesami Nigrum is pulverized, add to filtrate I, supersound extraction 40-50 minute, filter, obtain filtrate II and filtering residue II；
(6) filtrate II concentrating under reduced pressure is volatilized solvent, obtain extract II；
(7) extract I, extract II and filtering residue II mix homogeneously, it is dried, is Chinese medicine extract；
Wherein, in Ether-Acetone mixed liquor, ether and acetone volume ratio are 1:4；
Wherein, in step (3), the volume ratio of filtrate II and 1100 macroporous adsorbent resins is 1.5:1, the height of resin column and diameter
Ratio is 7:1.
A kind of dog grain improving immunity the most as claimed in claim 1, is characterized by: the preparation method of Radix Astragali Carnis Gallus domesticus is:
Fresh chicken shreds, and adds the Radix Astragali and water, and the mass ratio of Fresh chicken, the Radix Astragali and water is 1:0.5-0.7:4.5-5.5, decocts 3-4 little
Time, filtering, remaining filtering residue is Radix Astragali Carnis Gallus domesticus.
A kind of dog grain improving immunity the most as claimed in claim 1, is characterized by: the preparation method of Radix Astragali mushroom is:
Mushroom shredding, adds the Radix Astragali and water, and the mass ratio of mushroom, the Radix Astragali and water is 1:0.4-0.6:2.5-3.5, decocts 3-4 hour, mistake
Filter, remaining filtering residue is Radix Astragali mushroom；
Wherein mushroom be Lentinus Edodes, agrocyb eaegerita one or both.
A kind of dog grain improving immunity the most as claimed in claim 1, is characterized by:
Its preparation method is:
(1) rice and gluten meal mixer are mixed 10 minutes, after micronizing, standby；
(2) Chinese medicine extract, the Radix Astragali Carnis Gallus domesticus of pulverizing, the Os Gallus domesticus of pulverizing, the egg powder of pulverizing, the Carnis Sus domestica of pulverizing, pulverizing
Beef, the Radix Astragali mushroom of pulverizing, the Radix Dauci Sativae of pulverizing, the Fructus Cucurbitae moschatae of pulverizing, the Fructus Lycopersici esculenti of pulverizing, the Citrus meat of pulverizing, vitamin,
Mineral, zeolite powder, TYR, DL-methionine, 1B, taurine, potassium sorbate, antioxidant obtain with upper step
The material arrived mixes 10 minutes；
(3) mixed material being carried out expanded, 100-120 DEG C of baking oven of expanded rear entrance is dried；
(4) taking chicken oil, Adeps Bovis seu Bubali, soybean oil, Semen Maydis oil is put into spout pouring pot, dried material is carried out oil spout technique；
(5) material of after end of injection cools down, packaging, to obtain final product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106942504A (en) * | 2017-03-16 | 2017-07-14 | 上海耐威克宠物用品有限公司 | A kind of pet grain of heat-clearing and fire-reducing reinforced immunological and preparation method thereof |
| CN108041266A (en) * | 2017-11-22 | 2018-05-18 | 沂南帅克宠物用品有限公司 | A kind of pet food and preparation method for having effects that stomach conserves |
| CN110089638A (en) * | 2019-06-12 | 2019-08-06 | 江西喜保宠物用品有限公司 | A kind of pet food and preparation method thereof with health-care efficacy |
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| CN104489376A (en) * | 2014-12-19 | 2015-04-08 | 黄平县阿琳民族刺绣厂 | Intestine-stomach conditioning dog food |
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| CN105533220A (en) * | 2015-12-16 | 2016-05-04 | 济南昊泽环保科技有限公司 | Immunity enhancing traditional Chinese medicine meat dog feed |
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| CN102210405A (en) * | 2011-05-18 | 2011-10-12 | 天津中敖生物科技有限公司 | Astragalus-polyferose-containing premix for pigs and preparation method thereof |
| CN104489376A (en) * | 2014-12-19 | 2015-04-08 | 黄平县阿琳民族刺绣厂 | Intestine-stomach conditioning dog food |
| CN104825523A (en) * | 2015-04-20 | 2015-08-12 | 唐兴龙 | Wild chrysanthemum flower general flavone extraction method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106942504A (en) * | 2017-03-16 | 2017-07-14 | 上海耐威克宠物用品有限公司 | A kind of pet grain of heat-clearing and fire-reducing reinforced immunological and preparation method thereof |
| CN108041266A (en) * | 2017-11-22 | 2018-05-18 | 沂南帅克宠物用品有限公司 | A kind of pet food and preparation method for having effects that stomach conserves |
| CN110089638A (en) * | 2019-06-12 | 2019-08-06 | 江西喜保宠物用品有限公司 | A kind of pet food and preparation method thereof with health-care efficacy |
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