CN105054069A - Extraction method for flavones in marigold flower meal and application - Google Patents
Extraction method for flavones in marigold flower meal and application Download PDFInfo
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- CN105054069A CN105054069A CN201510570160.4A CN201510570160A CN105054069A CN 105054069 A CN105054069 A CN 105054069A CN 201510570160 A CN201510570160 A CN 201510570160A CN 105054069 A CN105054069 A CN 105054069A
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- CN
- China
- Prior art keywords
- flavones
- marigold flower
- extraction method
- coarse
- flower meal
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 229930003944 flavone Natural products 0.000 title claims abstract description 27
- 235000011949 flavones Nutrition 0.000 title claims abstract description 27
- 235000005881 Calendula officinalis Nutrition 0.000 title claims abstract description 21
- 150000002213 flavones Chemical class 0.000 title claims abstract description 15
- 240000000785 Tagetes erecta Species 0.000 title claims 3
- 238000000605 extraction Methods 0.000 title abstract description 5
- 235000012054 meals Nutrition 0.000 title abstract 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims abstract description 12
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 150000002212 flavone derivatives Chemical class 0.000 claims abstract description 8
- 241000287828 Gallus gallus Species 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 4
- 239000003674 animal food additive Substances 0.000 claims abstract description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 13
- 235000009566 rice Nutrition 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 240000007594 Oryza sativa Species 0.000 claims 2
- 241000736851 Tagetes Species 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 9
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 238000003307 slaughter Methods 0.000 abstract description 2
- 230000004060 metabolic process Effects 0.000 abstract 1
- 210000000056 organ Anatomy 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 19
- 241000209094 Oryza Species 0.000 description 11
- 235000012680 lutein Nutrition 0.000 description 8
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 8
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 description 8
- 229960005375 lutein Drugs 0.000 description 8
- 239000001656 lutein Substances 0.000 description 8
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 8
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 8
- 229930003935 flavonoid Natural products 0.000 description 6
- 150000002215 flavonoids Chemical class 0.000 description 6
- 235000017173 flavonoids Nutrition 0.000 description 6
- -1 flavone compound Chemical class 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 230000037323 metabolic rate Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 210000000579 abdominal fat Anatomy 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 208000002780 macular degeneration Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000974 natural food coloring agent Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000008601 oleoresin Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 210000002741 palatine tonsil Anatomy 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Fodder In General (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an extraction method for flavones in marigold flower meal and an application. The extraction method comprises the following steps: mixing marigold flower meal with ethanol with the concentration of 60 percent according to the material-liquid ratio of 1 to 20, and performing ultrasonic extraction under the ultrasonic temperature of 50 DEG C for 10 minutes to obtain a coarse flavone extract. The extracted flavones can be used as a broiler chicken feed additive, and the adding proportion is 40g/t. By adoption of ultrasonic assistance, flavones in the marigold flower meal are extracted; the operation is simple, convenient, accurate and high in extraction rate; the obtained coarse flavone extract can obviously improve the growth performance and the slaughter performance of broiler chicks, and the apparent metabolism of nutrients is enhanced; and the coarse flavone extract has certain immune and anti-oxidization effects on organs.
Description
Technical field
The present invention relates to the extractive technique field of flavones, be specifically related to extracting method and the application of flavones in a kind of marigold flower dregs of rice.
Background technology
Lutein and flavone compound is rich in marigold.Lutein (Lutein) has another name called " Lutein ", is a kind of natural food coloring.Research finds, lutein is a kind of excellent antioxidant, has effect of eyeshield, skin care, anti-cancer.Lutein also can be used as colouring agent to regulate the color and luster of Food & Drink.It also can be used as feed addictive and adds in bird feed.Often the edible food being rich in lutein, can prevent age related to look macular degeneration, to cataractous treatment or prevention effect, can regulate human immunological competence, pre-anti-cancer.Flavone compound (Flavonoids) is a general large class natural products distributed more widely in plant, mainly to be distributed in dicotyledon and gymnosperm main the combination with sugar of flavone compound in plant to exist with the form of glucosides, part flavone compound exists in a free form, because this compounds is to the scavenging action of active oxygen radical, there is angiocardiopathy preventing in vivo, cancer-resisting, immunity moderation, anti-ageing, anti-bacteria and anti-virus, anti-inflammatory antiallergy, many effects such as hemostatic analgesia, have been classified as a class of health food.The marigold flower dregs of rice are accessory substances of marigold processing oleoresin, it contains abundant active functional component especially Flavonoid substances, except extracting the lutein in marigold, have a large amount of marigold flower dregs of rice to be treated as industrial waste every year to abandon, this affects to environment, but also causes the very large wasting of resources.Therefore, extraction purification is carried out to the Flavonoid substances in the marigold flower dregs of rice, be conducive to the comprehensive utilization of marigold resource.
Summary of the invention
For solving the problem, the invention provides extracting method and the application of flavones in a kind of marigold flower dregs of rice.
For achieving the above object, the technical scheme that the present invention takes is:
In the marigold flower dregs of rice, an extracting method for flavones, comprises the steps:
By the marigold flower dregs of rice and concentration be 60% ethanol mix by solid-liquid ratio 1: 20 after, ultrasonic temperature 50 DEG C, ultrasonic extraction 10min, obtains flavone gruff bring up substance.
The flavones of said extracted can be used as broiler chicken feed additive and uses, and adding proportion is 40g/t.
The present invention has following beneficial effect:
Ultrasonic assistant is adopted to extract flavones in the marigold flower dregs of rice, simple to operation, accurate, recovery rate is high, the flavone gruff bring up substance of gained can significantly improve growth performance, the Slaughter of Broiler chicks, improves the apparent metabolic rate of nutrient, has certain immunity and antioxidation to body.
Detailed description of the invention
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiments provide the extracting method of flavones in a kind of marigold flower dregs of rice, comprise the steps:
By the marigold flower dregs of rice and concentration be 60% ethanol mix by solid-liquid ratio 1: 20 after, ultrasonic temperature 50 DEG C, ultrasonic extraction 10min, obtains flavone gruff bring up substance.
Embodiment
625 1 age in days healthy AA broiler chicken are divided into 5 processed group at random, often group establishes 5 repetitions, each repetition 25 chickens (male and female half and half), wherein 1 group is blank group, to feed Basic drawing, other 2,3,4,5 groups Basic drawing of feeding also add the present embodiment gained flavones CE 10g/t, 20g/t, 30g/t, 40g/t respectively, and whole experimental period is 42d altogether.
Growth performance: at 1-21d, test group average daily gain, average feed intake and feed-weight ratio and control group than equal difference not significantly (P > 0.05), are tested 4 groups of death rates and are reduced 65.12% (P < 0.05) than control group; At 22-42d, test group average daily gain and average feed intake with control group than significant difference (P < 0.05), test 3 groups of feed-weight ratio and reduce 4.78% (P < 0.05) with control group ratio, test 4,5 groups of death rates and reduce 59.31%, 60.47% (P < 0.05) with control group than respectively; At 1-42d, test the average daily gain of 5 groups and feed intake and control group score and do not improve 10.03%, 3.31% (P < 0.05), the feed-weight ratio control group testing 5 groups significantly reduces 6.01% (P < 0.05), test the death rate of 4 groups 59.67% (P < 0.05) lower than control group, not significantly (P > 0.05), wherein 40g/t crude flavonoid powder addition effect is more excellent for other each group difference.
Butchering property: test the clean thorax rate in 5 group Ban and improve 8.48% (P < 0.05) than control group, test 4 groups of abdominal fat and reduce 20.36% (P < 0.05) than control group, flavone gruff bring up substance affects difference significantly (P > 0.05) to dressing percentage, not significantly (P > 0.05), wherein 40g/t crude flavonoid powder addition effect is more excellent for other each group difference.
Nutrient apparent metabolic rate: test the crude protein of 5 groups, crude fat and phosphorus apparent metabolic rate and control group score does not improve 6.67%, 4.59%, 6.51% (P < 0.05), other each group difference is not significantly (P > 0.05); Between each group to the metabolic effect difference of dry, energy and calcium not significantly (P > 0.05).
Immunologic function: Thymus and Spleen index and the control group score of testing 5 groups do not improve 31.23%, 24.76% (P < 0.05), the legal system capsule index testing 4 groups improves 18.28% (P < 0.05) than control group, and the cecal tonsil index of test group and control group difference are not significantly (P > 0.05).Test 4 groups of IgA and IgG and control group score does not improve 26.66% and 16.77% (P < 0.05), each group difference of IgM is not significantly (P > 0.05).
Antioxidation: T-SOD and the T-SOD activity testing 5 groups does not improve 33.90%, 10.26% (P < 0.05) with control group score, and the GSH-PX specific activity control group testing 4 groups improves 24.61% (P < 0.05); Test 3 and 5 groups of MDA content and reduce 6.47%, 6.90% (P < 0.05) with control group than respectively, other each group difference is not significantly (P > 0.05).
6. economic benefit: test 4 and 5 groups and do not improve 15.59%, 18.59% (P < 0.05) with control group score, in test group, the economic benefit of the 5th group is the highest, namely interpolation 40g/t crude flavonoid powder effect is more excellent.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. the extracting method of flavones in the marigold flower dregs of rice, is characterized in that, comprise the steps:
By the marigold flower dregs of rice and concentration be 60% ethanol mix by solid-liquid ratio 1: 20 after, ultrasonic temperature 50 DEG C, ultrasonic extraction 10min, obtains flavone gruff bring up substance.
2. claim 1 application of flavones of extracting, it is characterized in that, use as broiler chicken feed additive, adding proportion is 40g/t.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510570160.4A CN105054069A (en) | 2015-09-01 | 2015-09-01 | Extraction method for flavones in marigold flower meal and application |
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| CN201510570160.4A CN105054069A (en) | 2015-09-01 | 2015-09-01 | Extraction method for flavones in marigold flower meal and application |
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| CN105054069A true CN105054069A (en) | 2015-11-18 |
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| CN201510570160.4A Pending CN105054069A (en) | 2015-09-01 | 2015-09-01 | Extraction method for flavones in marigold flower meal and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038652A (en) * | 2016-06-30 | 2016-10-26 | 卢月莲 | Method for extracting and purifying total flavonoids of chrysanthemum |
| CN110447766A (en) * | 2019-09-11 | 2019-11-15 | 广东省农业科学院动物科学研究所 | A kind of yellow-feather broiler fodder improving meat |
| WO2020073975A1 (en) * | 2018-10-12 | 2020-04-16 | 晨光生物科技集团股份有限公司 | Method for extracting marigold flavonoid in industrialized manner and marigold flavonoid thereof |
| CN116172201A (en) * | 2023-03-17 | 2023-05-30 | 浙江大学 | Tagetes extract and preparation method and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038652A (en) * | 2016-06-30 | 2016-10-26 | 卢月莲 | Method for extracting and purifying total flavonoids of chrysanthemum |
| WO2020073975A1 (en) * | 2018-10-12 | 2020-04-16 | 晨光生物科技集团股份有限公司 | Method for extracting marigold flavonoid in industrialized manner and marigold flavonoid thereof |
| CN110447766A (en) * | 2019-09-11 | 2019-11-15 | 广东省农业科学院动物科学研究所 | A kind of yellow-feather broiler fodder improving meat |
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| CN116172201A (en) * | 2023-03-17 | 2023-05-30 | 浙江大学 | Tagetes extract and preparation method and application thereof |
| CN116172201B (en) * | 2023-03-17 | 2024-05-10 | 浙江大学 | Tagetes extract and preparation method and application thereof |
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Application publication date: 20151118 |
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