CN106063820A - 一种连翘挥发油的提取工艺 - Google Patents

一种连翘挥发油的提取工艺 Download PDF

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CN106063820A
CN106063820A CN201610342675.3A CN201610342675A CN106063820A CN 106063820 A CN106063820 A CN 106063820A CN 201610342675 A CN201610342675 A CN 201610342675A CN 106063820 A CN106063820 A CN 106063820A
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volatile oil
forsythia volatile
extraction process
forsythia
take
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杨成东
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Nanjing Zelang Medical Technology Co Ltd
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Nanjing Zelang Medical Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/634—Forsythia
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/025—Recovery by solvent extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
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  • Organic Chemistry (AREA)
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  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

本发明公开了一种连翘挥发油的提取工艺，包括如下步骤：取连翘药材，粉碎筛选；加入纤维素酶酶解，喷洒pH4.5‑5.5的醋酸/醋酸钠缓冲溶液，达到手握成团即可；采用连续逆流超声提取，得到连翘挥发油。本发明制得的连翘挥发油25℃时的相对密度为0.6000‑0.92000，折光率为1.2000‑1.6000，旋光度为‑2°—+3°。本发明的提取方法简单易行，不需要大量的有机溶剂，安全无毒，成本低，污染小，适用于工业化生产。

Description

一种连翘挥发油的提取工艺
技术领域
本发明涉及中药技术领域，具体涉及一种连翘挥发油的提取工艺。
背景技术
连翘是木犀科连翘属植物，主要含苯乙醇苷类、木脂体及其苷类、五环三萜类、挥发油类等化合物。药理研究认为其具有抗菌、抗炎、解热、镇吐、利尿强心、抗肝损、镇痛、抑制磷酸二酯酶、抗病毒、降血压、抑制弹性蛋白酶活力、抗内毒素等作用。除果实入药，具清热解毒、消结排脓之效外，药用其叶，对治疗高血压、痢疾、咽喉痛等效果较好。
连翘对多种革兰阳性及阴性细菌均有抑制作用。其浓缩煎剂在体外可抑制伤寒杆菌、副伤寒杆菌、大肠杆菌、痢疾杆菌、白喉杆菌及霍乱弧菌、葡萄球菌、链球菌等，连翘在体外的抑菌作用与金银花大体相似，为银翘散中抗菌的主要成分。
发明内容
本发明的目的是提供一种连翘挥发油的提取工艺。
一种连翘挥发油的提取工艺，其特征在于，包括如下步骤：
a.取连翘药材，粉碎，过40目筛，再过100目筛，取截留物；
b.加入原料质量1-3%的纤维素酶，喷洒相当原料0.6-1.8倍的pH4.5-5.5的醋酸/醋酸钠缓冲溶液，达到手握成团即可；
c.取发酵后的原料，加入相当于原料质量4-6倍的60-70%的乙醇溶液，采用连续逆流超声提取，过滤，滤液经有机溶剂萃取、减压浓缩等步骤，得到连翘挥发油。
步骤b中所述的纤维素酶的活性为35-60U/mg。
步骤c中所述的超声频率45-60KHZ，于70~80℃的条件下提取2-3h。
步骤c中所述的有机溶剂为石油醚或***中的一种。
本发明具有安全无毒、耗时短、提取率高、工艺简单、可连续操作的优点。
具体实施方式：
以下通过实例对本发明进行阐述，但本发明的保护并不局限于此。
实施例1：
取连翘药材，粉碎，过40目筛，再过100目筛，取截留物，加入原料质量1%的纤维素酶（活性为30U/mg），喷洒相当原料0.6倍的pH4.5的醋酸/醋酸钠缓冲溶液，达到手握成团即可；取发酵后的原料，加入相当于原料质量4倍的60%的乙醇溶液，采用连续逆流超声提取法，于频率45KHz、温度70℃的条件下连续提取2h，过滤，滤液经石油醚萃取、减压浓缩等步骤，得到黄色的连翘挥发油，得率为2.1%。
实施例2：
取连翘药材，粉碎，过40目筛，再过100目筛，取截留物，加入原料质量3%的纤维素酶（活性为50U/mg），喷洒相当原料1.8倍的pH5.5的醋酸/醋酸钠缓冲溶液，达到手握成团即可；取发酵后的原料，加入相当于原料质量6倍的70%的乙醇溶液，采用连续逆流超声提取法，于频率60KHz、温度80℃的条件下连续提取3h，过滤，滤液经***萃取、减压浓缩等步骤，得到黄色的连翘挥发油，得率为3.2%。

Claims (4)

1.一种连翘挥发油的提取工艺，其特征在于，包括如下步骤：
a.取连翘药材，粉碎，过40目筛，再过100目筛，取截留物；
b.加入原料质量1-3%的纤维素酶，喷洒相当原料0.6-1.8倍的pH4.5-5.5的醋酸/醋酸钠缓冲溶液，达到手握成团即可；
c.取发酵后的原料，加入相当于原料质量4-6倍的60-70%的乙醇溶液，采用连续逆流超声提取，过滤，滤液经有机溶剂萃取、减压浓缩等步骤，得到连翘挥发油。
2.根据权利要求1所述的一种连翘挥发油的提取工艺，其特征在于，步骤b中所述的纤维素酶的活性为35-60U/mg。
3.根据权利要求1所述的一种连翘挥发油的提取工艺，其特征在于，步骤c中所述的超声频率45-60KHZ，于70~80℃的条件下提取2-3h。
4.根据权利要求1所述的一种连翘挥发油的提取工艺，其特征在于，步骤c中所述的有机溶剂为石油醚或***中的一种。
CN201610342675.3A 2016-05-23 2016-05-23 一种连翘挥发油的提取工艺 Pending CN106063820A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106511484A (zh) * 2016-12-07 2017-03-22 洛阳君山制药有限公司 一种复方金银花颗粒的提取制备工艺

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106511484A (zh) * 2016-12-07 2017-03-22 洛阳君山制药有限公司 一种复方金银花颗粒的提取制备工艺
CN106511484B (zh) * 2016-12-07 2019-07-05 洛阳君山制药有限公司 一种复方金银花颗粒的提取制备工艺

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Application publication date: 20161102