CN106035393A - Seed germination promoter for weakening low-temperature inhibition - Google Patents

Seed germination promoter for weakening low-temperature inhibition Download PDF

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Publication number
CN106035393A
CN106035393A CN201610354575.2A CN201610354575A CN106035393A CN 106035393 A CN106035393 A CN 106035393A CN 201610354575 A CN201610354575 A CN 201610354575A CN 106035393 A CN106035393 A CN 106035393A
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China
Prior art keywords
parts
temperature
low
seeds
water
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CN201610354575.2A
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Chinese (zh)
Inventor
周承东
程圆
李德琴
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Anhui Huahe Seed Industry Co Ltd
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Anhui Huahe Seed Industry Co Ltd
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Priority to CN201610354575.2A priority Critical patent/CN106035393A/en
Publication of CN106035393A publication Critical patent/CN106035393A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/20—Copper
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a seed germination promoter for weakening low-temperature inhibition. The seed germination promoter is prepared from the following raw materials in parts by weight: 15-17 parts of furfural residue, 10-12 parts of tea seed cakes, 3-4 parts of sodium citrate, 2-3 parts of copper sulfate, 3-4 parts of calcium carbonate, 0.2-0.4 part of 5-aminolevulinic acid, 54-56 parts of fresh crofton weed, 18-19 parts of fresh oysters, 1.3-1.5 parts of pseudomonas aeruginosa agent, 2.5-2.7 parts of high-temperature cellulose-decomposing bacterium agent, a proper amount of 6mol/L hydrochloric acid solution and a proper amount of water. The germination promoter disclosed by the invention can be used for enhancing the low-temperature resistance of seeds; by adding 5-aminolevulinic acid and the like, the activity of antioxidant enzymes in embryos is improved, so that the damage to cell membranes caused by active oxygen is reduced, and the adverse effect on seed germination caused by low temperature is relieved; and by adding a variety of efficient nutrients in a matched manner, the rapid germination of the seeds under low-temperature conditions is achieved, so that the germination promoter is applicable to the culture and planting of the seeds in low-temperature regions.

Description

A kind of presprouting of seeds agent weakening low temperature suppression
Technical field
The present invention relates to agricultural Stratification technique field, particularly relate to a kind of presprouting of seeds agent weakening low temperature suppression.
Background technology
Along with the development of agricultural, the fast seedling growing of seed becomes the important act improving Agriculture, forestry And Animal Husbandry yield of commercial crops Arrange, and seed because of external environment be not suitable for or own growth is immature, the seed dormancy phase length that causes, germination percentage is low, sprout The situations such as irregular and germination quality is low, have had a strong impact on the growth of later stage crop, and result in production cycle length, product matter Measure the most high series of problems, affect economic benefit.And germinator can effectively solve the problems referred to above, but current accelerating germination skill Art many employings plant growth regulator carries out accelerating germination, and not only means are single, simultaneously not for the real needs of all kinds of seeds, nothing Method carries out effective strain to environment, kind etc., causes the waste of resource and the undesirable of specifically used effect.
Eupatorium cannabinum L. (Eupatorium coelestinum L.) is herbaceos perennial, is that a kind of vitality is strong, that breeding is difficult to clean off rapidly, again is pernicious miscellaneous Grass, it discharges allelochemical, suppression other plant growth by root system secretion, rainfall leaching and Residue decay to soil, is formed Single dominantcommunity, causes the underproduction of agriculture forest and husbandry, endangers huge.And at present utilization to it focuses mostly on entered by its allelopathy Row sterilization or control plant growing, research and utilization to its lixiviating solution that becomes thoroughly decomposed are the most relatively fewer.
Polyamines has bioactive small molecule aliphatic in being present in higher plant, fungus and prokaryote body Non-protein nitrogenous bases compound, higher at the biological in-vivo content of rich in proteins, polyamines can affect RNA polymerization in plumular axis The activity of enzyme, induces seed germination by promoting the synthesis of protein, makes it regulate seed on nucleic acid and protein level Sprout.
Owing to the Eupatorium cannabinum L. (Eupatorium coelestinum L.) lixiviating solution that becomes thoroughly decomposed is rich in available nutrients such as humic acids and nitrogen, phosphorus, potassium, calcium, magnesium and trace element, It is compounded together with the polyamines extracted in Concha Ostreae and forms the preparation with Germination effect, agricultural cultivation has development greatly Potentiality, the most also the large-scale development for presprouting of seeds nursery industry provides new technical measures.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of presprouting of seeds weakening low temperature suppression Agent.
The present invention is achieved by the following technical solutions:
A kind of presprouting of seeds agent weakening low temperature suppression, is prepared by the raw materials in: furfural dregs 15-17, tea seed cake 10- 12, sodium citrate 3-4, copper sulfate 2-3, calcium carbonate 3-4,5-ALA 0.2-0.4, fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) 54-56, fresh Concha Ostreae 18-19, P. aeruginosa bacteria agent 1.3-1.5, high temperature fiber decomposer microbial inoculum 2.5-2.7,6mol/L hydrochloric acid solution are suitable Amount, water are appropriate.
Specifically comprising the following steps that of the described presprouting of seeds agent preparation method weakening low temperature suppression
(1) shred after fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) water being rinsed, mix with furfural dregs, tea seed cake in input compost pond, be separately added into copper Green pseudomonas microbial inoculum and high temperature fiber decomposer microbial inoculum, and keep ferment heap water content to ferment 20-22 days in 50-55%, retting, Air-drying fermentation material after fermentation ends, cross 1.0-1.2mm sieve after pulverizing, the powder that must become thoroughly decomposed is standby；
(2) Concha Ostreae is shelled take meat and blend, minced meat is mixed by solid-liquid ratio 1:1.5-1.6g/ml with 6mol/L hydrochloric acid solution, After being heated to 55-60 DEG C insulation extract 180-200 minute, be placed on afterwards in centrifuge, under 3000-3100 rev/min from Heart 28-32 minute, crosses leaching supernatant after being centrifuged, regulation PH is to neutral standby；
(3) become thoroughly decomposed step 1 gained to add after powder, sodium citrate mix with copper sulfate in the water of 6-8 times of compound weight and soak Steep 4-6 hour, add calcium carbonate and 5-ALA afterwards, be warming up to 62-65 DEG C and be stirred continuously to fully dispersed, then Stand to room temperature, nutritional solution must be chelated after filtering with double gauze standby；
(4) step 2 gained feed liquid, step 3 gained chelating nutritional solution and other leftover materials are sufficiently mixed uniformly, add water Solution is diluted 20-22 times, obtains presprouting of seeds agent of the present invention.
The invention have the advantage that
The present invention, by the compound preparation of polyamines extracted in become thoroughly decomposed Eupatorium cannabinum L. (Eupatorium coelestinum L.) lixiviating solution and Concha Ostreae, can reach detoxification, Stimulation germination and the active matter of growth of seedling can be synthesized again, promote the starch in seed, protein and inositolophosphate Hydrolysis, thus contribute to the macro-nutrients hydrolysis in seed endosperm and become little molecule necessary to plant structure plant Nutrient substance, thus induce the sprouting of seed, and the H that polyamine oxidase degraded produces2O2By participating in cellular redox state The effects such as regulation promote cell wall ripe and hardening, further increase germination percentage and the seedling growth rate of seed.
The germinator of the present invention can strengthen the low-temperature resistance of seed, by the interpolation of 5-ALA etc., improves The activity of antioxidant enzyme in embryo, thus reduce the active oxygen injury to cell membrane, alleviate low temperature and seed germination is caused Adverse effect, coordinate the interpolation of multiple high efficiency nutritious material, it is achieved that the fast-germination of seed under cryogenic conditions, it is adaptable to low The seed culture in temperature area and plantation.
Detailed description of the invention
A kind of presprouting of seeds agent weakening low temperature suppression, is made up of the raw material of following weight portion (kg): furfural dregs 15, tea seed cake 10, sodium citrate 3, copper sulfate 2, calcium carbonate 3,5-ALA 0.2, fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) 54, fresh Concha Ostreae 18, Aerugo vacation Zymomonas mobilis microbial inoculum 1.3, high temperature fiber decomposer microbial inoculum 2.5,6mol/L hydrochloric acid solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the described presprouting of seeds agent preparation method weakening low temperature suppression
(1) shred after fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) water being rinsed, mix with furfural dregs, tea seed cake in input compost pond, be separately added into copper Green pseudomonas microbial inoculum and high temperature fiber decomposer microbial inoculum, and keep ferment heap water content 50%, retting is fermented 20 days, fermentation knot Air-drying fermentation material after bundle, cross 1.0mm sieve after pulverizing, the powder that must become thoroughly decomposed is standby；
(2) Concha Ostreae is shelled take meat and blend, minced meat is mixed by solid-liquid ratio 1:1.5g/ml with 6mol/L hydrochloric acid solution, heating After 55 DEG C, insulation is extracted 180 minutes, is placed on afterwards in centrifuge, is centrifuged 28 minutes under 3000 revs/min, centrifugal complete Rear leaching supernatant of crossing, regulation PH is to neutral standby；
(3) become thoroughly decomposed step 1 gained to add after powder, sodium citrate mix with copper sulfate in the water of 6 times of compound weight and soak 4 Hour, add calcium carbonate and 5-ALA afterwards, be warming up to 62 DEG C and be stirred continuously to fully dispersed, then standing to often Temperature, must chelate nutritional solution after filtering with double gauze standby；
(4) step 2 gained feed liquid, step 3 gained chelating nutritional solution and other leftover materials are sufficiently mixed uniformly, add water Solution is diluted 20 times, obtains presprouting of seeds agent of the present invention.
In order to further illustrate the using value of the present invention, inventor chooses batch of seeds and is equally divided into two groups, one group of work Use the germinator made of common plant growth regulator for matched group, another group use germinator of the present invention as experimental group, During seed culture, temperature controls at 5-10 DEG C, germinates found that experimental group seed relatively matched group carries the last week, and germinates Rate reaches 86%, and matched group only has 53%, and the seed germination of experimental group is neat simultaneously, and planting bud quality relatively matched group has and significantly carry Rise, it is seen that germinator of the present invention has preferable using effect.

Claims (2)

1. the presprouting of seeds agent weakening low temperature suppression, it is characterised in that be prepared by the raw materials in: furfural dregs 15-17, tea seed cake 10-12, sodium citrate 3-4, copper sulfate 2-3, calcium carbonate 3-4,5-ALA 0.2-0.4, the most light violet Stem Herba Lycopi 54-56, fresh Concha Ostreae 18-19, P. aeruginosa bacteria agent 1.3-1.5, high temperature fiber decomposer microbial inoculum 2.5-2.7, 6mol/L hydrochloric acid solution is appropriate, water is appropriate.
2. according to the presprouting of seeds agent weakening low temperature suppression described in claims 1, it is characterised in that preparation method concrete Step is as follows:
(1) shred after fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) water being rinsed, mix with furfural dregs, tea seed cake in input compost pond, be separately added into copper Green pseudomonas microbial inoculum and high temperature fiber decomposer microbial inoculum, and keep ferment heap water content to ferment 20-22 days in 50-55%, retting, Air-drying fermentation material after fermentation ends, cross 1.0-1.2mm sieve after pulverizing, the powder that must become thoroughly decomposed is standby；
(2) Concha Ostreae is shelled take meat and blend, minced meat is mixed by solid-liquid ratio 1:1.5-1.6g/ml with 6mol/L hydrochloric acid solution, After being heated to 55-60 DEG C insulation extract 180-200 minute, be placed on afterwards in centrifuge, under 3000-3100 rev/min from Heart 28-32 minute, crosses leaching supernatant after being centrifuged, regulation PH is to neutral standby；
(3) become thoroughly decomposed step 1 gained to add after powder, sodium citrate mix with copper sulfate in the water of 6-8 times of compound weight and soak Steep 4-6 hour, add calcium carbonate and 5-ALA afterwards, be warming up to 62-65 DEG C and be stirred continuously to fully dispersed, then Stand to room temperature, nutritional solution must be chelated after filtering with double gauze standby；
(4) step 2 gained feed liquid, step 3 gained chelating nutritional solution and other leftover materials are sufficiently mixed uniformly, then add Solution is diluted 20-22 times by water, obtains presprouting of seeds agent of the present invention.
CN201610354575.2A 2016-05-26 2016-05-26 Seed germination promoter for weakening low-temperature inhibition Pending CN106035393A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115152546A (en) * 2022-06-29 2022-10-11 海南广陵高科实业有限公司 High-yield and high-quality cultivation method for southern-bred crop corn

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102795900A (en) * 2012-08-29 2012-11-28 攀枝花市西宇生物科技有限公司 Method for preparing bio-organic fertilizer by utilizing eupatorium adenophorum

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102795900A (en) * 2012-08-29 2012-11-28 攀枝花市西宇生物科技有限公司 Method for preparing bio-organic fertilizer by utilizing eupatorium adenophorum

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
付玉营 等: "多胺对种子萌发生长及成熟的影响", 《种子》 *
郑晓菊 等: "高效液相色谱法测定贝壳类海洋生物中的多胺含量", 《青岛科技大学学报（自然科学版）》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115152546A (en) * 2022-06-29 2022-10-11 海南广陵高科实业有限公司 High-yield and high-quality cultivation method for southern-bred crop corn

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