CN106035246A - Method for cultivating larvae of sipunculus nudus indoors - Google Patents
Method for cultivating larvae of sipunculus nudus indoors Download PDFInfo
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- CN106035246A CN106035246A CN201610500667.7A CN201610500667A CN106035246A CN 106035246 A CN106035246 A CN 106035246A CN 201610500667 A CN201610500667 A CN 201610500667A CN 106035246 A CN106035246 A CN 106035246A
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- 241000237854 Sipunculus nudus Species 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000195493 Cryptophyta Species 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000001418 larval effect Effects 0.000 claims abstract description 12
- 230000000638 stimulation Effects 0.000 claims abstract description 7
- 230000029052 metamorphosis Effects 0.000 claims abstract description 6
- 230000000384 rearing effect Effects 0.000 claims description 17
- 239000004576 sand Substances 0.000 claims description 16
- 238000005286 illumination Methods 0.000 claims description 14
- 235000013339 cereals Nutrition 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 11
- 235000013360 fish flour Nutrition 0.000 claims description 11
- 241000721619 Najas Species 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 7
- 230000012447 hatching Effects 0.000 claims description 6
- 210000004602 germ cell Anatomy 0.000 claims description 5
- 238000009933 burial Methods 0.000 claims description 4
- 244000144987 brood Species 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 6
- 230000006698 induction Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000029087 digestion Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 abstract 1
- 238000007654 immersion Methods 0.000 abstract 1
- 230000008569 process Effects 0.000 abstract 1
- 230000003203 everyday effect Effects 0.000 description 15
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 9
- 230000004720 fertilization Effects 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 241000227752 Chaetoceros Species 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 230000032696 parturition Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 241000206751 Chrysophyceae Species 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000237927 Golfingia Species 0.000 description 1
- 241001501873 Isochrysis galbana Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for cultivating larvae of sipunculus nudus indoors. The method mainly comprises the steps of parent selection, spawn induction, larval cultivation, nyad cultivation and the like. Compared with the prior art, the method has the beneficial effects that (1) the emission ratio and synchronicity of parent sperms and eggs can be improved by using a 5-hydroxytryptamine solution immersion and drying-in-the-shade stimulation combined induction method; (2) algae are fed when individual pelagospheric larvae start to hatch, so that the survival rate of pelagospheric larvae can be increased; (3) water is not changed in the process of cultivating larvae, and the water quality is controlled by reasonably controlling the larval density and the bait amount; (4) benthic diatom is cultivated in a larval pool in advance, sufficient nutrition is provided to larvae which are just attached, and the metamorphosis survival rate and growth rate of larvae can be increased; and (5) larval baits are subjected to superfine grinding, so that digestion of larvae can be benefited. The method can be used for improving the yield of the larvae so as to increase the economic benefit, and has the advantages of simple operation, easy popularization and the like.
Description
Technical field
The present invention relates to aquaculture technology, more particularly, to the method for a kind of Sipunculus nudus indoor seed rearing.
Background technology
Sipunculus nudus (Sipunculus nudus) it is Genus Golfingia common species, the latent husky troglodytism in Intertidal zone, be
Important regional precious marine product.Constantly being raised up by the market price and affected, the catching intensity of Sipunculus nudus increasingly sharpens, and adds Shanghai
Ocean environmental change and beach destroy, and Sipunculus nudus resource significantly reduces.At present, star is carried out on the ground such as the North Sea, Zhanjiang the most in various degree
Worm cultivates, but seed source still relies primarily on the wild population fished for, and seed yield is the most unstable with quality, and causes nature
The heavy damage of resource.According to statistics, the only suitable cultured area of area, Guangdong and Guangxi Provinces Sipunculus nudus i.e. reaches more than 200,000 mu, if having big
Amount high-quality seed supply market, Sipunculus nudus is expected to become aquaculture new varieties and marine industry new growth point.
Sipunculus nudus seed rearing mainly includes that outdoor earth pond is cultivated and cultivates with indoor water mud sump, wherein indoor water mud sump training
The technology of educating relates generally to parent culture, larval culture cultivates link with young Seedling；Cultivating with outdoor earth pond and compare, indoor water mud sump is trained
Educate mainly by controlling parent's accelerating, germling density, the aspects such as bait and aquaculture management of throwing something and feeding, improve the rate of growth of germling, one-tenth
Motility rate and distortion ratio, thus improve seed yield and economic benefit, present in prior art in the breeding method of Sipunculus nudus,
The defect that existence yields poorly, the cultivation time is short.
Summary of the invention
It is an object of the invention to the drawbacks described above overcoming prior art to exist, it is provided that the seed training of a kind of Sipunculus nudus indoor
The method educated.
It is an object of the invention to be achieved by the following technical programs:
The method of a kind of Sipunculus nudus indoor seed rearing, comprises the following steps:
S1. by the end of April to mid-September, selecting healthy individuals as parent, in parent, male and female number ratio is 5～10:1, will
Parent puts into discharge in parent pond and joins after soaking a period of time, then dry in the shade stimulation in the 5-hydroxy tryptamine solution of 0.01%～0.15%
Son；Germ cell to be found starts hatching and goes to sea after ball germling, algae of throwing something and feeding；After incubating oosperm 2～3 days, sea ball germling is turned
Cultivate in moving to germling pond, algae of simultaneously throwing something and feeding, control illumination；
Lay larva pond the most in advance: laying burial depth in larva pond is 2～5cm, and grains of sand footpath is 100～1000 m, in children
Extra large ball germling in body pond proceeds to before larva pond 7～10 days, cultivates benthic diatom in larva pond；
S3. the extra large ball germling after being cultivated in germling pond by S1 proceeds in larva pond described in S3, and described sea ball germling is in larva pond
Interior density is 0.2～0.5/mL, algae of simultaneously throwing something and feeding, and controls illumination；
S4. after the extra large ball larval metamorphosis in the larva pond in S3 adheres to, starting mixed feed of throwing something and feeding, feeding volume is 3～5g/
m3, until growing up to naiad.
Here, parent is chosen as: selected reproduction season for by the end of April to mid-September, before lunar calendar spring tide 2～3 days from cultivation
Colony selects bores the strongest husky healthy individuals as parent, and parent individual specification is 15～25g/ tails.
Preferably, S1 germ cell to be found starts hatching and goes to sea after ball germling, and the feeding volume of algae is 1.0～1.5 × 104
Individual/mL.
Preferably, parent is soaked in the 5-hydroxy tryptamine solution of 0.01%～0.15% by S1 10～15min, described in dry in the shade
The time stimulated is 30～60min.
Preferably, in parent pond described in S1, burial depth is 10～15cm, and grains of sand footpath is 200～2000 m.
Preferably, in S1, the density of inland sea, germling pond ball germling is 0.5～1.0/mL, the feeding volume of algae be 2.0～
2.5×104Individual/mL, illumination controls 2000～2500Lux.
Preferably, in S3, the feeding volume of algae is 3.0～4.5 × 104Individual/mL, illumination controls 2000～2500Lux.
Preferably, mixed feed described in S4 is made up of Kelp Powder, Alga Sgrgassi Enerves powder and fish flour, wherein Kelp Powder, Alga Sgrgassi Enerves powder
It is (4～6) with the mass ratio of fish flour: (4～6): (0.5～1.5).
It is highly preferred that the mass ratio of described Kelp Powder, Alga Sgrgassi Enerves powder and fish flour is 5:4:1.
Preferably, the feeding volume of mixed feed described in S4 is 3～5g/m3；The particle diameter of mixed feed is less than 50 m.
Preferably, it is characterised in that described brood pond, germling pond, larva pond are indoor water mud sump.
Compared with prior art, the method have the advantages that
The method that the invention provides a kind of Sipunculus nudus indoor seed rearing, it has the beneficial effects that: (1) uses 5-hydroxy tryptamine
The abductive approach that solution soaking combines with dry in the shade stimulation, improves emission index and the synchronicity of parent's essence ovum；(2) indivedual sea ball childrens
Body starts hatching, algae of i.e. throwing something and feeding, and improves sea ball germling survival rate；(3) during larval culture, water is not changed, by rationally control
Germling density processed controls water quality with the abundance of food；(4) larva pond pre-incubation benthic diatom, the larva for just attachment provides abundance
Nutrition, improves metamorphosis and survival rate and the speed of growth of larva；(5) larva bait carries out micronizing, beneficially larva digestion；This
Bright described method can improve seed yield, thus improves economic benefit, and has the advantages such as easy and simple to handle, easy popularization.
Detailed description of the invention
Further illustrate present disclosure below in conjunction with specific embodiment, but should not be construed as the limit to the present invention
System.In the case of present invention spirit and essence, the amendment that the inventive method, step, condition are made or replacement, all belong to
In the scope of the present invention.Unless otherwise noted, experimental technique used in embodiment is well known to the skilled person
Conventional method and technology, reagent or material are and are obtained by commercial sources.
In the specific embodiment of the invention, parent pond, germling pond, larva pond are cement pit, and it constructs reference application number
It it is the patent of invention of 201510193494.4.
Embodiment 1
On May 11st, 2014 carries out Sipunculus nudus indoor seed rearing, specifically comprises the following steps that
(1) parent selects: select healthy individuals as parent from East Sea Island, Zhanjiang City Sipunculus nudus cultured population, specification (16 ±
4.3) g/ tail, female: male ratio 10:1.
(2) induction parent discharges gamete: first the parent selected utilizes 5-hydroxy tryptamine solution soaking 10min of 0.05%,
Dry in the shade stimulation 40min；Putting into parent pond to hasten parturition, parent pond grains of sand layer thickness is 10cm, the grains of sand a diameter of 200～2000 m, water
Temperature is (28 ± 2.3) DEG C, and rate of fertilization is 86%.
(3) after fertilization is observed at any time, and 33h finds the whip gold such as germ cell starts hatching and goes to sea ball germling, Zhanjiang of now throwing something and feeding
Algae, cloud microalgae and chlorella, every day feeding volume 1.2 × 104Individual/ml.
(4) larval culture in early days: May 13, transfers to sea ball germling germling pond and cultivates, 0.7/ml of germling density,
Throw something and feed lsochrysis zhanjiangensis, cloud microalgae and chlorella, every day feeding volume 2.3 × 104Individual/ml, illumination controls to be 2200lux, no
Change water.
(5) benthic diatom is cultivated: lays larva pond, larva pond grains of sand layer thickness 2cm, grains of sand footpath 500 m, starts to cultivate the end
Dwell diatom, and benthic diatom is mainly wedge shape algae, avette algae, rhombus algae.
(5) later stage larvae cultivation: May 20, proceeds to cultivate the larva pond of benthic diatom, larva by later stage sea ball germling
0.3/ml of density, lsochrysis zhanjiangensis of throwing something and feeding, cloud microalgae, chlorella and Chaetoceros, every day feeding volume 3.0 × 104Individual/ml,
Illumination controls as 2200lux.
(6) naiad is cultivated: May 23, and germling starts abnormal attachment, and after metamorphosis completes, survival rate is 47%, and every day throws something and feeds super
Fine Kelp Powder, Alga Sgrgassi Enerves powder and fish flour, Kelp Powder, Alga Sgrgassi Enerves powder, the mass ratio of fish flour are 5:4:1, bait particle diameter
Less than 50 m, feeding volume is 5g/m3, change water 20% every day, after 60 days, naiad goes out pond and carries out sea area cultivation.
Embodiment 2
On May 2nd, 2015 carries out Sipunculus nudus indoor seed rearing, specifically comprises the following steps that
(1) parent selects: select the Sipunculus nudus of health as parent, rule from Cao Tan Zhenhai District, Suixi County, Zhanjiang City cultured population
Lattice (18 ± 3.2) g/ tail, female: male ratio 7:1.
(2) induction parent discharges gamete: the parent selected utilizes 5-hydroxy tryptamine solution soaking 15min of 0.07%, dries in the shade
Putting into parent pond after stimulating 30min to hasten parturition, parent pond grains of sand layer thickness is 10cm, the grains of sand a diameter of 200～2000 m, water temperature
For (29 ± 1.2) DEG C, rate of fertilization is 90%.
(3) after fertilization is observed at any time, hatches primary sea ball germling after 40h, and incubation rate is 92%, Zhanjiang etc. of now throwing something and feeding
Whip chrysophyceae, cloud microalgae and chlorella, every day feeding volume 1.4 × 104Individual/ml.
(4) larval culture in early days: siphon sea ball germling transfers to germling pond, 0.6/ml of germling density, Zhanjiang etc. of throwing something and feeding
Whip chrysophyceae, cloud microalgae and chlorella, every day feeding volume 2.0 × 104Individual/ml, illumination controls to be 2300lux, does not change water.
(4) benthic diatom is cultivated: lay larva pond, fine sand thickness 3cm, fine sand particle diameter 800 m, May as in Example 1
5, starting to cultivate benthic diatom, benthic diatom is mainly wedge shape algae, avette algae, rhombus algae.
(5) later stage larvae cultivation: May 13, proceeds to cultivate the larva pond of benthic diatom, germling by later stage sea ball larva
0.5/ml of density, lsochrysis zhanjiangensis of throwing something and feeding, cloud microalgae, chlorella and Chaetoceros, every day feeding volume 4.0 × 104Individual/ml,
Illumination controls as 2300lux.
(6) naiad is cultivated: May 18, germling started abnormal attachment, and after metamorphosis completes, survival rate is 42%, and every day throws something and feeds super
Fine Kelp Powder, Alga Sgrgassi Enerves powder and fish flour, Kelp Powder, Alga Sgrgassi Enerves powder, the mass ratio of fish flour are 5:4:1, bait particle diameter
Less than 50 m, feeding volume is 4g/m3.Change water 30% every day.After cultivating 20 days, naiad density is 5600/m2, average weight
0.019g/ bar.
Embodiment 3
On June 10th, 2015 carries out Sipunculus nudus indoor seed rearing, specifically comprises the following steps that
(1) parent selects: select the Sipunculus nudus of health as parent, specification from Cao Tan town, Suixi County, Zhanjiang City cultured population
(17 ± 4.2) g/ tail, female: male ratio 8:1.
(2) induction parent discharges gamete: utilize the 5-hydroxy tryptamine solution soaking parent 20min of 0.10%, dry in the shade stimulation
50min；Putting into parent pond afterwards to hasten parturition, parent pond grains of sand layer thickness is 10cm, and the grains of sand a diameter of 200～2000 m, water temperature is
(29 ± 2.2) DEG C, rate of fertilization is 80%.
(3) after fertilization is observed at any time, hatches primary sea ball germling, a length of 260um of average body, Zhan of now throwing something and feeding after 30h
River Isochrysis galbana, cloud microalgae and chlorella, every day feeding volume 1.5 × 104Individual/ml.
(4) larval culture in early days: draw germling and transfer to germling pond；1.0/ml of germling density, the whip such as Zhanjiang of throwing something and feeding gold
Algae, cloud microalgae and chlorella, every day feeding volume 2.5 × 104Individual/ml, illumination controls to be 2500lux, does not change water.
(4) benthic diatom is cultivated: as embodiment 1 lays larva pond, fine sand thickness 5cm, fine sand particle diameter 1000 m, June 13
Day, starting to cultivate benthic diatom, benthic diatom is mainly wedge shape algae, avette algae, rhombus algae.
(5) later stage larva educates: 10d phase sea ball germling body a length of (450 ± 26) um, proceeds to later stage sea ball larva cultivate
The larva pond of benthic diatom, 0.5/ml of larval density, lsochrysis zhanjiangensis of throwing something and feeding, cloud microalgae, chlorella and Chaetoceros, every day
Feeding volume 4.5 × 104Individual/ml, illumination controls as 2500lux.
(6) naiad is cultivated: June 23, germling started abnormal attachment, adhered to germling body a length of (825 ± 28) um, and every day throws
Feeding the Kelp Powder of micronizing, Alga Sgrgassi Enerves powder and fish flour, Kelp Powder, Alga Sgrgassi Enerves powder, the mass ratio of fish flour are 5:4:1, bait
Particle diameter is less than 50 m, and feeding volume is 4g/m3, change water 30% every day, after 30 days, naiad average body length reaches 1.2cm.
Claims (9)
1. the method for a Sipunculus nudus indoor seed rearing, it is characterised in that comprise the following steps:
S1. by the end of April to mid-September, selecting healthy individuals as parent, in parent, male and female number ratio is 5～10:1, will
Parent puts into discharge in parent pond and joins after soaking a period of time, then dry in the shade stimulation in the 5-hydroxy tryptamine solution of 0.01%～0.15%
Son；Germ cell to be found starts hatching and goes to sea after ball germling, algae of throwing something and feeding；After incubating oosperm 2～3 days, sea ball germling is turned
Cultivate in moving to germling pond, algae of simultaneously throwing something and feeding, control illumination, do not change water；
Lay larva pond the most in advance: laying burial depth in larva pond is 2～5cm, and grains of sand footpath is 100～1000 m, in children
Extra large ball germling in body pond proceeds to before larva pond 7～10 days, cultivates benthic diatom in larva pond；
S3. the extra large ball germling after being cultivated in germling pond by S1 proceeds in larva pond described in S3, and described sea ball germling is in larva pond
Interior density is 0.2～0.5/mL, algae of simultaneously throwing something and feeding, and controls illumination；
S4. after the extra large ball larval metamorphosis in the larva pond in S3 adheres to, starting mixed feed of throwing something and feeding, feeding volume is 3～5g/
m3, until growing up to naiad.
The method of Sipunculus nudus indoor the most according to claim 1 seed rearing, it is characterised in that in S1, parent is existed
Soaking 10～15min in the 5-hydroxy tryptamine solution of 0.01%～0.15%, the time of described dry in the shade stimulation is 30～60min.
The method of Sipunculus nudus indoor the most according to claim 1 seed rearing, it is characterised in that S1 germ cell to be found
Starting hatching and go to sea after ball germling, the feeding volume of algae is 1.0～1.5 × 104Individual/mL.
The method of Sipunculus nudus indoor the most according to claim 1 seed rearing, it is characterised in that in parent pond described in S1
Burial depth is 10～15cm, and grains of sand footpath is 200～2000 m.
The method of Sipunculus nudus indoor the most according to claim 1 seed rearing, it is characterised in that inland sea, germling pond in S1
The density of ball germling is 0.5～1.0/mL, and the feeding volume of algae is 2.0～2.5 × 104Individual/mL, illumination control 2000～
2500Lux。
The method of Sipunculus nudus indoor the most according to claim 1 seed rearing, it is characterised in that in S3, algae throws something and feeds
Amount is 3.0～4.5 × 104Individual/mL, illumination controls 2000～2500Lux.
The method of Sipunculus nudus indoor the most according to claim 1 seed rearing, it is characterised in that mixed feed described in S4
Being made up of Kelp Powder, Alga Sgrgassi Enerves powder and fish flour, wherein the mass ratio of Kelp Powder, Alga Sgrgassi Enerves powder and fish flour is (4～6): (4～6):
(0.5～1.5).
The method of Sipunculus nudus indoor the most according to claim 7 seed rearing, it is characterised in that mixed feed described in S4
Feeding volume be 3～5g/m3。
9. according to the method for the Sipunculus nudus indoor seed rearing described in any one of claim 1 to 8, it is characterised in that described
Brood pond, germling pond, larva pond are indoor water mud sump.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610500667.7A CN106035246B (en) | 2016-06-30 | 2016-06-30 | A kind of method of seed rearing in Sipunculus nudus room |
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| CN201610500667.7A CN106035246B (en) | 2016-06-30 | 2016-06-30 | A kind of method of seed rearing in Sipunculus nudus room |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165404A (en) * | 2020-03-07 | 2020-05-19 | 广西壮族自治区海洋研究所 | Indoor pond ecological polyculture method for sipunculus nudus, ostrea rivularis and golden drum fish |
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| US20020069833A1 (en) * | 2000-07-05 | 2002-06-13 | Tech Master S.A. | Water circulation apparatus and method |
| CN101589698A (en) * | 2009-04-01 | 2009-12-02 | 福建省水产研究所 | The artificial raise seedling method of nude Sipunculus nudus |
| CN102835356A (en) * | 2012-09-21 | 2012-12-26 | 广西壮族自治区海洋研究所 | Breeding method of artificial parents of sipunculus nudus |
| CN102960276A (en) * | 2012-11-20 | 2013-03-13 | 汕头大学 | Cultivation method of Chlamys nobilis new strain enriched with DNA (Deoxyribonucleic Acid) and application thereof |
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2016
- 2016-06-30 CN CN201610500667.7A patent/CN106035246B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020069833A1 (en) * | 2000-07-05 | 2002-06-13 | Tech Master S.A. | Water circulation apparatus and method |
| CN101589698A (en) * | 2009-04-01 | 2009-12-02 | 福建省水产研究所 | The artificial raise seedling method of nude Sipunculus nudus |
| CN102835356A (en) * | 2012-09-21 | 2012-12-26 | 广西壮族自治区海洋研究所 | Breeding method of artificial parents of sipunculus nudus |
| CN102960276A (en) * | 2012-11-20 | 2013-03-13 | 汕头大学 | Cultivation method of Chlamys nobilis new strain enriched with DNA (Deoxyribonucleic Acid) and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈振国: "方格星虫土池育苗技术规程", 《海洋与渔业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165404A (en) * | 2020-03-07 | 2020-05-19 | 广西壮族自治区海洋研究所 | Indoor pond ecological polyculture method for sipunculus nudus, ostrea rivularis and golden drum fish |
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| CN106035246B (en) | 2019-09-10 |
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