CN106035057A - Zucchini net room seed production method - Google Patents
Zucchini net room seed production method Download PDFInfo
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- CN106035057A CN106035057A CN201610361044.6A CN201610361044A CN106035057A CN 106035057 A CN106035057 A CN 106035057A CN 201610361044 A CN201610361044 A CN 201610361044A CN 106035057 A CN106035057 A CN 106035057A
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- flower
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a zucchini net room seed production method. An improved female and male parent planting mode is adopted in a net room, zoning relay intercropping of male and female parents on cultivation ridges of a double-concave structure is realized, the male parent does not occupy extra land solely, the objectives of increasing female parent planting plants on total area of a seed production land parcel and improving male and female parent plant growing environment are achieved, and improving of yield and quality of zucchini hybrid seeds is facilitated. The yield of the zucchini hybrid seeds is increased by 26.1%-28.6%, and thousand seed weight is increased by 10.1-10.3 g.
Description
Technical field
The present invention relates to a kind of Cucurbita pepo L. solarium producing method for seed, belong to vegetable breeding technical field.
Background technology
Cross-bred seeding for summer squash is for the purpose of producing high-quality hybrid seed, utilizes the advantage that Cucurbita pepo L. male parent is maternal
Dominant, by producing in yielding ability, disease resistance, resistance the Hybrid significantly more than parents after the artificial hybridization production of hybrid seeds.
It is on the low side to there is yield in Cucurbita pepo L. during hybrid seeding, and seed quality and purity are difficult to effectively control, and production cost is high
Problem, these problems constrain the development of Cucurbita pepo L. kind industry to a certain extent.
The technological deficiency of the Cucurbita pepo L. tradition conventional hybridization production of hybrid seeds is mainly manifested in:
(1) planting in the male parent of Cucurbita pepo L., maternal the most individually partition set, male parent takies independent land area, reduces system
Plant plant number maternal on the gross area of plot, cause hybrid seed to yield poorly.
(2) in order to reduce male parent floor space, generally use the method plantation male parent strengthening planting density, cause male parent to plant
Disorderly competition between strain, makes the male flower of plant can not get more preferable nutritional condition, and it is poor that male flower is grown, and pollen quantity reduces, unfavorable
Adopt powder in carrying out, cause hybridization pollination of poor quality.
(3) roguing opportunity and method can not science, Parent hybrid strain removes thorough, causes the generation of pseudostationary, hybridization
Seed purity declines.
(4) must arrange remote isolation area, multiple bumpkin hybrid kinds can not be in same base closely breeding, and breeding becomes
This change is big.
Therefore, how to improve the systematic seeding technique of Cucurbita pepo L., reduce the generation of pseudostationary, improve hybrid seed yield,
Reducing breeding cost is the problem demanding prompt solution that the industry is faced.
Summary of the invention
The technical problem to be solved is for problem above, it is provided that a kind of Cucurbita pepo L. solarium producing method for seed, should
It is high that method adds the planting of maternal strain number on the gross area of production of hybrid seeds plot, seed purity and percentage of seedgermination；Improve Parent
Planting proportion, reduces male parent planting density, is conducive to improving hybrid seed yield and quality, reduces breeding cost.
For solving above technical problem, the Cucurbita pepo L. solarium producing method for seed of the present invention, comprise the following steps:
1) solarium is set: select the rich soil that high-land, irrigation and drainage are good, and the plot without quarantine disease sets up arc
Frame, span 6-8m, rise 2.5-3.0m, long 60-80m, arc bow member overlying lid 40-50 mesh fly net；
2) cultivation ridge is set: arranging cultivation ridge in solarium, cultivation ridge is double concave type structure, is protruded band and 2 hollow zones by 3
Composition, 3 protrude bandwidth and are 20cm, protrude band height 20cm, and 2 depression bandwidth are 20cm, the deep 20cm of hollow zone, and adjacent 2
Bar cultivation distance between the ridges 150cm；
3) use solarium in Shandong be April early and middle ten days carry out the Cucurbita pepo L. production of hybrid seeds, the ratio of Parent is 1:5, and male parent is more Zao than maternal
Sowing 5-7 days, the seedling age phase is about 20 days, and rice shoot reaches to carry out field planting during 3 leaf 1 heart, male parent field planting is being cultivated in the middle of ridge
Protrude on band, maternal field planting on 2 protrusion bands of cultivation both sides, ridge, the typical case such as leaf, leaf color, growing way according to Seedling before field planting
Character pair father, female parent carry out the 1st time and go hybrid strain, the young plant of hybrid strain and doubtful hybrid strain are removed, no longer field planting；
4) female parent is hung climing cultivation, when maternal 1st female flower is buddingged, according to typical case's character such as beginning Hua Jiewei, alabastrum color
Female parent is carried out the 2nd time and goes hybrid strain；By the 1st female flower pollination self of female parent, sit melon, it is simple to go hybrid strain the 3rd time；Maternal 1st
Female flower is sat after melon 8-10 days, carries out the 3rd time going hybrid strain according to typical case's character pair female parent such as curcumbitate, melon skin color, goes hybrid strain the 3rd time
Rear in time by female parent sugar-preserved gourd and the 2nd female flower alabastrum excision；Hybridization pollination is started from the 3rd maternal female flower；
5) except male flower: front 1 afternoon of pollinating extracts maternal male flower and double property flower, it is ensured that maternal during pollination do not have open male flower
With double property flower.
6) folder flower: the male flower clip of the 2nd day maternal unisexuality female flower to be opened and male parent is clamped by front 1 afternoon of pollinating；
7) pollination: when rear 2nd day 8-11 in the morning spent by folder, taken off by the female clip taken, opens petal, and the male parent plucking folder flower is male
Flower, removes petal and exposes flower pesticide, be evenly coated on female chapiter by male flower pollen and hybridize, the most again with clip by female flower
Petal clamp；
8), after all pollination terminates, paternal plant is pulled out；Pluck fruit, flower, alabastrum and the side of having no result do not pollinated on maternal plant
Branch, stays 2-3 sheet leaf pinching in last hybridization side of taking；
9), when hybridization melon length is to 3-4cm size, selects fruit shape 2-3 melon good, supermature to make kind of a melon, unnecessary melon is removed；
Kind melon growth course carries out the 4th to female parent and goes hybrid strain, the female parent that kind of melon does not meets varietal character is removed in time；Plant melon to become
Gather in time after ripe, after carrying out after-ripening in 7-10 days, take seed.
A kind of prioritization scheme, in described step 3) during field planting, female parent strain is away from for 40cm, and male parent spacing in the rows is 100cm.
Another kind of prioritization scheme, in described step 7), a strain retains 3-4 hybridization female flower, and namely a strain female parent ensures have
3-4 colored hybridization pollination.
Another prioritization scheme, also hangs climing cultivation to male parent in described step 4), the 1st female flower alabastrum occurs in paternal plant
After, carry out male parent according to typical case's character such as beginning Hua Jiewei, alabastrum color and go hybrid strain for the 2nd time, and by the 1st female flower pollination self,
Sitting melon, the 1st female flower is sat after melon 8-10 days, carries out the 3rd time going hybrid strain according to typical case's character pair male parent such as curcumbitate, melon skin color,
Extract after 3rd time is gone hybrid strain and in time the 1st melon that female flower is sat of male parent.
Use above technical scheme, compared with prior art, have the advantage that
(1) the Cucurbita pepo L. production of hybrid seeds of the present invention is by innovation cultivation ridge structure in solarium, changes Parent under tradition normal condition independent
Subregion nonoculture plantation is mixing point band relay interplanting planting, the not extra individually land occupation of male parent, adds on the gross area of production of hybrid seeds plot
Planting of maternal strain number, improve bumpkin hybrid seed production.667m of the present invention2Bumpkin hybrid seed production is more traditional often
Rule method improves 26.1%-28.6%.
(2) the Cucurbita pepo L. production of hybrid seeds of the present invention changes dense planting in male parent partition set is a point band dispersion thin planting, male parent robust growth, is formed
Male flower quantity is many, quality good；Meanwhile, be conducive to improving Parent planting proportion, be effectively improved the strain number of male parent, improve
The quality of pollen and supply, improve the quality of hybridization pollination.The more traditional routine side of bumpkin hybrid thousand grain weigth of the present invention
Method increases 10.1-10.3g, and seed is fuller.
(3) method by staying fruit to identify hybrid strain has been innovated in the Cucurbita pepo L. production of hybrid seeds of the present invention, it is possible to thoroughly remove Parent miscellaneous
Strain, substantially increases the purity of hybrid seed, and seed purity reaches 100%；Percentage of seedgermination is 98.8%-100%, and field is miscellaneous without vacation
Strain occurs.
(4) present invention uses solarium's production of hybrid seeds, need not arrange remote isolation area, can realize multiple bumpkin hybrid kind same
Base closely breeding.
Below in conjunction with detailed description of the invention, the invention will be further described.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to protection scope of the present invention.
Embodiment 1, field test is in vegetable breeding base, Shouguang City.Pumpkin variety is the selection-breeding of company of Shouguang City
First cross kind green grass or young crops calabash No. 1, male parent is SHL01-05, and female parent is SHL03-22.
Test method: test has set 2 contrasts, for the conventional producing method for seed of tradition and producing method for seed of the present invention.
Solarium of the present invention producing method for seed step:
1) solarium is set: select the rich soil that high-land, irrigation and drainage are good, and the plot without quarantine disease sets up arc
Frame, span 6-8m, rise 2.5-3.0m, long 60-80m, arc bow member overlying lid 40-50 mesh fly net.
2) cultivation ridge is set: arranging cultivation ridge in solarium, cultivation ridge is double concave type structure, recessed by 3 protrusion bands and 2
Falling into band composition, 3 protrude bandwidth and are 20cm, protrude band height 20cm, and 2 depression bandwidth are 20cm, the deep 20cm of hollow zone, phase
Adjacent 2 cultivation distance between the ridges 150cm.
3) use solarium in Shandong be April early and middle ten days carry out blue or green No. 1 Cucurbita pepo L. production of hybrid seeds of calabash, the ratio of Parent is 1:5, father
This was than maternal planting early enough 5-7 days, and the seedling age phase is about 20 days, and rice shoot reaches to carry out field planting during 3 leaf 1 heart, male parent field planting was being planted
On protrusion band in the middle of banking up, spacing in the rows is 100cm.Maternal field planting is on 2 protrusion bands of cultivation both sides, ridge, and spacing in the rows is 40cm.
Before field planting, typical case character pair father, the female parent such as leaf, leaf color, growing way according to Seedling carry out the 1st time going hybrid strain, hybrid strain and doubtful miscellaneous
The young plant of strain is removed, no longer field planting.
4) female parent is hung climing cultivation, when maternal 1st female flower is buddingged, according to typical cases such as beginning Hua Jiewei, alabastrum colors
Character pair female parent carries out the 2nd time and goes hybrid strain；By the 1st female flower pollination self of female parent, sit melon, it is simple to go hybrid strain the 3rd time；Maternal the
1 female flower is sat after melon 8-10 days, carries out the 3rd time going hybrid strain according to typical case's character pair female parent such as curcumbitate, melon skin color, goes for the 3rd time
In time female parent sugar-preserved gourd and the 2nd female flower alabastrum are extractd after hybrid strain；Hybridization pollination is started from the 3rd maternal female flower.Male parent is hung
Climing cultivation, after the 1st female flower alabastrum occurs in paternal plant, carries out male parent the 2nd according to typical case's character such as beginning Hua Jiewei, alabastrum color
Secondary hybrid strain of going, and by the 1st female flower pollination self, sit melon, the 1st female flower is sat after melon 8-10 days, according to curcumbitate, melon skin color etc.
Typical case's character pair male parent carries out the 3rd time and goes hybrid strain, after the 3rd time is gone hybrid strain and extract the 1st melon that female flower is sat of male parent in time.
5) except male flower: the pollinate front 1 maternal male flower of afternoon excision and double property flowers, it is ensured that during pollination, female parent does not open
Male flower and double property flower.
6) folder flower: the male flower clip of the 2nd day maternal unisexuality female flower to be opened and male parent is clamped by front 1 afternoon of pollinating；
7) pollination: when rear 2nd day 8-11 in the morning spent by folder, taken off by the female clip taken, opens petal, and the male parent plucking folder flower is male
Flower, removes petal and exposes flower pesticide, be evenly coated on female chapiter by male flower pollen and hybridize, the most again with clip by female flower
Petal clamp；One strain female parent retains 3-4 hybridization female flower.
8), after all pollination terminates, paternal plant is pulled out；Pluck fruit, flower, alabastrum and the nothing do not pollinated on maternal plant
Really side shoot, stays 2-3 sheet leaf pinching in last hybridization side of taking.
9), when hybridization melon length is to 3-4cm size, selects fruit shape 2-3 melon good, supermature to make kind of a melon, unnecessary melon is gone
Fall；Kind melon growth course carries out the 4th to female parent and goes hybrid strain, kind of melon is not met the maternal timely of blue or green No. 1 varietal character of calabash
Remove；Gather in time after planting melon maturation, after carrying out after-ripening in 7-10 days, take seed.
Above-mentioned go hybrid strain process, be intended to pull out the hybrid strain in male parent, planting of maternal row and doubtful hybrid strain.
Result of the test: present invention producing method for seed 667m more conventional than tradition2SHL03-22(is maternal) to increase plant number be 440
Strain, blue or green No. 1 bumpkin hybrid seed 667m of calabash2Output increased 28.6%, single melon amount of setting seeds increases by 1.7, and mass of 1000 kernel increases
10.3g。
From the present invention and the made cenospecies of tradition routine producing method for seed, randomly draw 2000 seeds respectively, carry out blue or green calabash
No. 1 field planting test, No. 1 percentage of seedgermination of production of hybrid seeds green grass or young crops calabash of the present invention is 98.8%, and field occurs without false hybrid strain, seed purity
Reach 100%；Conventional No. 1 percentage of seedgermination of production of hybrid seeds green grass or young crops calabash of tradition is 92.3%, and field has the false hybrid strain of 0.35% to occur.
Embodiment 2, field test is in vegetable breeding base, Shouguang City.Pumpkin variety is the selection-breeding of company of Shouguang City
First cross kind green grass or young crops calabash No. 3, male parent is SHL01-07, and female parent is SHL03-24.
Test method: test has set 2 contrasts, for the conventional producing method for seed of tradition and producing method for seed of the present invention.
Solarium of the present invention producing method for seed step: in addition to kind difference, other measure is with embodiment 1.
Result of the test: present invention producing method for seed 667m more conventional than tradition2SHL03-24(is maternal) to increase plant number be 440
Strain, blue or green No. 3 bumpkin hybrid seed 667m of calabash2Output increased 26.1%, single melon amount of setting seeds increases by 1.6, and mass of 1000 kernel increases
10.1g。
From the present invention and the made cenospecies of tradition routine producing method for seed, randomly draw 2000 seeds respectively, carry out blue or green calabash
No. 3 field planting tests, No. 3 percentages of seedgermination of production of hybrid seeds green grass or young crops calabash of the present invention are 100%, and field occurs without false hybrid strain, and seed purity reaches
100%；Conventional No. 3 percentages of seedgermination of production of hybrid seeds green grass or young crops calabash of tradition are 92.5%, and field has the false hybrid strain of 0.31% to occur.
By the experimental result of above example, it can be seen that taking the present invention to carry out the Cucurbita pepo L. production of hybrid seeds, production of hybrid seeds plot is total
On area, female parent strain number increases, and paternal pollen is grown, maternal stalwartness of growing, and single melon amount of setting seeds dramatically increases, and mass of 1000 kernel is obvious
Increasing, seed is full, and germination percentage is high, and seed purity is high, and yield is high.
The above is the citing of best mode for carrying out the invention, and it is common that the part addressed the most in detail is this area
The common knowledge of technical staff.Protection scope of the present invention is as the criterion with the content of claim, any technology based on the present invention
The equivalent transformation enlightened and carry out, also within protection scope of the present invention.
Claims (4)
1. a Cucurbita pepo L. solarium producing method for seed, it is characterised in that include production of hybrid seeds following steps:
1) solarium is set: select the rich soil that high-land, irrigation and drainage are good, and the plot without quarantine disease sets up arc
Frame, span 6-8m, rise 2.5-3.0m, long 60-80m, arc bow member overlying lid 40-50 mesh fly net；
2) cultivation ridge is set: arranging cultivation ridge in solarium, cultivation ridge is double concave type structure, is protruded band and 2 hollow zones by 3
Composition, 3 protrude bandwidth and are 20cm, protrude band height 20cm, and 2 depression bandwidth are 20cm, the deep 20cm of hollow zone, and adjacent 2
Bar cultivation distance between the ridges 150cm；
3) use solarium in Shandong be April early and middle ten days carry out the Cucurbita pepo L. production of hybrid seeds, the ratio of Parent is 1:5, and male parent is more Zao than maternal
Sowing 5-7 days, the seedling age phase is about 20 days, and rice shoot reaches to carry out field planting during 3 leaf 1 heart, male parent field planting is being cultivated in the middle of ridge
Protruding on band, maternal field planting, on 2 protrusion bands of cultivation both sides, ridge, carries out the 1st time go hybrid strain to father, female parent before field planting；
4) female parent is hung climing cultivation；Carry out female parent the 2nd time when 1st female flower is buddingged going hybrid strain；By the 1st female flower selfing
Pollination, sitting melon, the 1st female flower carries out the 3rd time for 8-10 days after sitting melon and goes hybrid strain, go for the 3rd time after hybrid strain in time by female parent sugar-preserved gourd with
2nd female flower alabastrum is extractd, and starts hybridization pollination from the 3rd female flower；
5) except male flower: front 1 afternoon of pollinating extracts maternal male flower and double property flower, it is ensured that maternal during pollination do not have open male flower
With double property flower；
6) folder flower: the male flower clip of the 2nd day maternal unisexuality female flower to be opened and male parent is clamped by front 1 afternoon of pollinating；
7) pollination: when rear 2nd day 8-11 in the morning spent by folder, taken off by the female clip taken, opens petal and exposes stigma, plucks folder flower
Male parent male flower, remove petal and expose flower pesticide, pollen is evenly coated on female chapiter and hybridizes, the most again with clip will
The petal of female flower is clamped；
8), after all pollination terminates, paternal plant is pulled out；Pluck fruit, flower, alabastrum and the side of having no result do not pollinated on maternal plant
Branch, stays 2-3 sheet leaf pinching in last hybridization side of taking；
9), when hybridization melon length is to 3-4cm size, selects fruit shape 2-3 melon good, supermature to make kind of a melon, unnecessary melon is removed；
Kind melon growth course carries out the 4th to female parent and goes hybrid strain；Gather in time after planting melon maturation, after carrying out after-ripening in 7-10 days, take kind
Son.
A kind of Cucurbita pepo L. solarium the most according to claim 1 producing method for seed, it is characterised in that: in step 3) during field planting, female
This spacing in the rows is 40cm, and male parent spacing in the rows is 100cm.
A kind of Cucurbita pepo L. solarium the most according to claim 1 producing method for seed, it is characterised in that: in step 7), a strain retains 3-
4 hybridization female flowers.
A kind of Cucurbita pepo L. solarium the most according to claim 1 producing method for seed, it is characterised in that: male parent is also hung by step 4)
Climing cultivation, plant carries out the 2nd time to male parent after there is the 1st female flower alabastrum and goes hybrid strain, by the 1st female flower pollination self, sits melon,
1st female flower carries out the 3rd time to male parent in 8-10 days after sitting melon and goes hybrid strain, is extractd by melon in time after going hybrid strain for the 3rd time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610361044.6A CN106035057A (en) | 2016-05-29 | 2016-05-29 | Zucchini net room seed production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610361044.6A CN106035057A (en) | 2016-05-29 | 2016-05-29 | Zucchini net room seed production method |
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| CN106035057A true CN106035057A (en) | 2016-10-26 |
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ID=57175070
Family Applications (1)
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| CN201610361044.6A Pending CN106035057A (en) | 2016-05-29 | 2016-05-29 | Zucchini net room seed production method |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303731A (en) * | 2014-08-06 | 2015-01-28 | 山西省农业科学院棉花研究所 | Zucchini breed hybrid seed production method |
| CN104429536A (en) * | 2014-12-08 | 2015-03-25 | 寿光市新世纪种苗有限公司 | Cultivation method capable of controlling tomato root knot nematode |
-
2016
- 2016-05-29 CN CN201610361044.6A patent/CN106035057A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303731A (en) * | 2014-08-06 | 2015-01-28 | 山西省农业科学院棉花研究所 | Zucchini breed hybrid seed production method |
| CN104429536A (en) * | 2014-12-08 | 2015-03-25 | 寿光市新世纪种苗有限公司 | Cultivation method capable of controlling tomato root knot nematode |
Non-Patent Citations (2)
| Title |
|---|
| 汪炳良: "《蔬菜制种百问百答》", 30 April 2009 * |
| 金庆忠: "春季棚室西葫芦间作苦瓜栽培技术", 《科学种养》 * |
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