CN106011129B - Microbial nucleic acids extracting process - Google Patents
Microbial nucleic acids extracting process Download PDFInfo
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- CN106011129B CN106011129B CN201610602706.4A CN201610602706A CN106011129B CN 106011129 B CN106011129 B CN 106011129B CN 201610602706 A CN201610602706 A CN 201610602706A CN 106011129 B CN106011129 B CN 106011129B
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- microorganism
- filter membrane
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- nucleic acids
- microbial nucleic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
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- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of microbial nucleic acids extracting process, include the following steps：A, microorganism fluid sample is prepared：The sample containing microorganism is added first in the culture dish for filling nutrient solution, and jiggled uniformly, it is spare that liquid sample is made；B, microbe filter：Liquid sample in culture dish is poured slowly on filter membrane, the microorganism in liquid sample is made to be attached to the surface of filter membrane；C, microorganism shifts：Filter membrane is placed in solution, and cleans filter membrane repeatedly, the microorganism of filter membrane surface is made to be transferred in solution；D, microbial nucleic acids extract：Solution is heated 10~15 minutes with 85~95 DEG C, makes microorganism is molten to split and its nucleic acid is made to be released in solution.Microbial nucleic acids extracting process of the invention is easy to operate, it is quick to extract, and can effectively come out the nucleic acid extraction in microorganism, in case subsequently carrying out development activities use, guarantee early period is provided for the correlation properties of people's microorganisms.
Description
Technical field
The present invention relates to microorganism abstraction technique field, more particularly to a kind of microbial nucleic acids extracting process.
Background technology
Microorganism individual is small, type is various, and very close with the relationship of the mankind, both include photosynthetic bacteria, lactic acid bacteria,
The microorganism beneficial to the mankind such as saccharomycete, while also including micro- life to human hazard such as salmonella, streptococcus, virus
Object.It is either beneficial or harmful, microorganism all play the role of to the production and scientific research activity of the mankind it is vital, therefore,
Understand the correlation properties with microorganisms, so that it may rationally to utilize the advantage of microorganism, it be avoided to bring our harm.
It is well known that nucleic acid is not only basic inhereditary material, and also take up in the biosynthesis of protein important
Position, thus serve in a series of great biological phenomenas such as biological growth, heredity, variation conclusive.Therefore, it studies micro-
The hereditary capacity of biotinylated nucleic acid, be that we understand the breach of microorganism in depth, and study nucleic acid is primarily first from microorganism
Middle extraction nucleic acid.
Invention content
The present invention is that nucleic acid is extracted from microorganism, discloses a kind of microbial nucleic acids extracting process, includes the following steps：
A, microorganism fluid sample is prepared：The sample containing microorganism is added first in the culture dish for filling nutrient solution,
And jiggle uniformly, it is spare that liquid sample is made；
B, microbe filter：Liquid sample in culture dish is poured slowly on filter membrane, is made in the liquid sample
Microorganism is attached to the surface of filter membrane；
C, microorganism shifts：The filter membrane is placed in solution, and cleans filter membrane repeatedly, makes the micro- of filter membrane surface
Biology is transferred in solution；
D, microbial nucleic acids extract：The solution is heated 10~15 minutes with 85~95 DEG C, makes microorganism is molten to split
And its nucleic acid is made to be released in the solution, to complete the extraction of microbial nucleic acids.
As a further improvement on the present invention, the nutrient solution by following parts by weight material composition：
Urea 1~2%, sodium acetate 0.1~0.2%, amino acid 3~5%, borax 1~2%, glucose 8~10%, sugar
Honey 1~3%, surplus is water.
As a further improvement on the present invention, the filter membrane is the cellulose mixture filter membrane in 0.3~0.5 μm of aperture.
As a further improvement on the present invention, the solution is sterile distilled water.
The beneficial effects of the invention are as follows：
Microbial nucleic acids extracting process of the invention is easy to operate, extraction is quick, can be effectively by the core in microorganism
Acid extraction comes out, and is used in case subsequently carrying out development activities, and guarantee early period is provided for the correlation properties of people's microorganisms.
Description of the drawings
It to describe the technical solutions in the embodiments of the present invention more clearly, below will be to needed in the embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for ability
For the those of ordinary skill of domain, without having to pay creative labor, others are can also be obtained according to these attached drawings
Attached drawing.
Fig. 1 is the microorganism extraction process block diagram of the present invention.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
As shown in Figure 1, the microbial nucleic acids extracting process of the present invention, includes the following steps：
A, microorganism fluid sample is prepared：The sample containing microorganism is added first in the culture dish for filling nutrient solution,
And jiggle uniformly, it is spare that liquid sample is made；
B, microbe filter：Liquid sample in culture dish is poured slowly on filter membrane, is made in the liquid sample
Microorganism is attached to the surface of filter membrane；
C, microorganism shifts：The filter membrane is placed in solution, and cleans filter membrane repeatedly, makes the micro- of filter membrane surface
Biology is transferred in solution；
D, microbial nucleic acids extract：The solution is heated 10~15 minutes with 85~95 DEG C, makes microorganism is molten to split
And its nucleic acid is made to be released in the solution, to complete the extraction of microbial nucleic acids.
Wherein, nutrient solution by following parts by weight material composition：
Urea 1~2%, sodium acetate 0.1~0.2%, amino acid 3~5%, borax 1~2%, glucose 8~10%, sugar
Honey 1~3%, surplus is water.
Filter membrane is the cellulose mixture filter membrane in 0.3~0.5 μm of aperture, so that microorganism is preferably trapped within filter membrane
Surface；Solution is sterile distilled water, to reduce being mixed into for other miscellaneous bacterias.
Microbial nucleic acids extracting process of the invention is easy to operate, extraction is quick, can be effectively by the core in microorganism
Acid extraction comes out, and is used in case subsequently carrying out development activities, and guarantee early period is provided for the correlation properties of people's microorganisms.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (3)
1. microbial nucleic acids extracting process, which is characterized in that include the following steps：
A, microorganism fluid sample is prepared：The sample containing microorganism is added first in the culture dish for filling nutrient solution, and light
Jog shakes uniformly, and it is spare that liquid sample is made；
B, microbe filter：Liquid sample in culture dish is poured slowly on filter membrane, micro- life in the liquid sample is made
Object is attached to the surface of filter membrane；
C, microorganism shifts：The filter membrane is placed in solution, and cleans filter membrane repeatedly, makes the microorganism of filter membrane surface
It is transferred in solution；
D, microbial nucleic acids extract：The solution is heated 10~15 minutes with 85~95 DEG C, makes microorganism is molten to split and make
Its nucleic acid is released in the solution, to complete the extraction of microbial nucleic acids；
The nutrient solution by following parts by weight material composition：
Urea 1~2%, sodium acetate 0.1~0.2%, amino acid 3~5%, borax 1~2%, glucose 8~10%, molasses 1~
3%, surplus is water.
2. microbial nucleic acids extracting process according to claim 1, which is characterized in that the filter membrane be aperture 0.3~
0.5 μm of cellulose mixture filter membrane.
3. microbial nucleic acids extracting process according to claim 1, which is characterized in that the solution is sterile distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610602706.4A CN106011129B (en) | 2016-07-27 | 2016-07-27 | Microbial nucleic acids extracting process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610602706.4A CN106011129B (en) | 2016-07-27 | 2016-07-27 | Microbial nucleic acids extracting process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106011129A CN106011129A (en) | 2016-10-12 |
| CN106011129B true CN106011129B (en) | 2018-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610602706.4A Expired - Fee Related CN106011129B (en) | 2016-07-27 | 2016-07-27 | Microbial nucleic acids extracting process |
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| Country | Link |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI671407B (en) * | 2018-10-31 | 2019-09-11 | 康儀科技股份有限公司 | Method for extracting nucleic acid of microorganism |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824769A (en) * | 2005-02-25 | 2006-08-30 | 中国农业科学院哈尔滨兽医研究所 | Pig streptococcus bacterial strain and its use |
| CN101580877A (en) * | 2009-06-24 | 2009-11-18 | 南开大学 | Oligonucleotide microarray technique for detecting pathogen contamination in seawater |
| CN102548568A (en) * | 2009-01-14 | 2012-07-04 | 全技术公司 | Clay interlaced yeast compositions and methods of utilizing the same |
| CN104450862A (en) * | 2014-11-04 | 2015-03-25 | 广西大学 | Sugarcane top rot resistance identification method |
-
2016
- 2016-07-27 CN CN201610602706.4A patent/CN106011129B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824769A (en) * | 2005-02-25 | 2006-08-30 | 中国农业科学院哈尔滨兽医研究所 | Pig streptococcus bacterial strain and its use |
| CN102548568A (en) * | 2009-01-14 | 2012-07-04 | 全技术公司 | Clay interlaced yeast compositions and methods of utilizing the same |
| CN101580877A (en) * | 2009-06-24 | 2009-11-18 | 南开大学 | Oligonucleotide microarray technique for detecting pathogen contamination in seawater |
| CN104450862A (en) * | 2014-11-04 | 2015-03-25 | 广西大学 | Sugarcane top rot resistance identification method |
Non-Patent Citations (2)
| Title |
|---|
| 海洋药物导论 第2版;张文;《海洋药物导论 第2版》;上海科学技术出版社;20120930;55页 * |
| 现代生物技术原理及其应用;叶勤;《现代生物技术原理及其应用》;中国轻工业出版社;20030831;201-202页 * |
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| CN106011129A (en) | 2016-10-12 |
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Granted publication date: 20180817 Termination date: 20190727 |