CN106007950B - Application of bacillus LY152 in preparation of microbial fertilizer - Google Patents
Application of bacillus LY152 in preparation of microbial fertilizer Download PDFInfo
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- CN106007950B CN106007950B CN201610339599.0A CN201610339599A CN106007950B CN 106007950 B CN106007950 B CN 106007950B CN 201610339599 A CN201610339599 A CN 201610339599A CN 106007950 B CN106007950 B CN 106007950B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to application of bacillus LY152 in preparation of microbial fertilizer, and firstly discovers that bacillus strains can secrete 3-indoleacetic acid by utilizing nutrient components in the fertilizer. The bacillus LY152 has the Latin nameBacillus sp.And 8.25.8.2014, which is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC NO 9541. The prepared microbial fertilizer comprises a bacillus LY152 microbial inoculum, nutrient components, an auxiliary agent and a filler. The microbial fertilizer is rich in nutrition and beneficial to microbial growth, colonization and crop absorption. The microorganism can rapidly propagate and secrete 3-indoleacetic acid, stimulate the growth of crops to ensure that the microorganism can fully absorb and utilize the nutrients in the fertilizer, has obvious yield increasing effect, can improve the soil micro-ecology and enhance the disease resistance of the crops.
Description
The technical field is as follows:
the invention relates to the field of microbial fertilizer preparation, in particular to a method for preparing a microbial fertilizer by utilizing a bacterial strain capable of producing indoleacetic acid and application thereof.
Background art:
china breeds 22% of people in the world in cultivated land 7% of the world, and the use work of chemical fertilizers is unavailable. Chemical fertilizers are important agricultural production materials. The fertilizer plays an irreplaceable role in promoting the development of grain and agricultural production, but the problems of excessive application, blind application and the like of the fertilizer exist at present, which brings about the increase of cost and the pollution to the environment. The 'zero-increase action of fertilizer usage amount' implemented by related departments enables the traditional agriculture to be transformed into ecological agriculture more and more quickly, and the emergence of the ecological agriculture promotes the development and application of microbial fertilizers. The microbial fertilizer is non-toxic and harmless, does not pollute the environment, can increase the content of nitrogen or effective phosphorus and potassium in soil through the life metabolic activity of microorganisms, or can adjust the level of crop hormone substances or inhibit the activity of plant pathogenic bacteria, thereby improving the soil fertility, improving the nutritional condition and the growth environment of crops and increasing the crop yield. The use amount of chemical fertilizer can be reduced by using the microbial fertilizer, so that the deterioration of the ecological environment of the soil can be effectively relieved.
Therefore, the seeking of using the high-efficiency microbial fertilizer to improve the utilization rate of the fertilizer, reduce the use amount of the fertilizer and the pesticide, improve the ecological environment and realize the sustainable development of agriculture is an urgent task of agriculture in China at present. Although there are some microbial fertilizers on the market today, such as: azotobacterin fertilizer, phosphorus bacterial fertilizer, potassium bacterial fertilizer, etc. The fertilizers have the problems of large dosage, high cost and the like, and the application effect of some products is unstable.
Indole-acetic acid (indole-3-acetic acid) is a main active ingredient of natural auxin and is involved in many processes of plant development. Microorganisms can promote plant growth by secreting indoleacetic acid. The IAA can increase the size and distribution of plant roots and is beneficial to the roots to absorb more nutrients from the soil. The production of plant hormones is one of the main mechanisms of the interaction between microbes and host plants, and the strains are easy to colonize and play roles in plants. Therefore, the method has important significance in producing the novel microbial fertilizer by using the strain with the function of secreting 3-indoleacetic acid, adding other components and designing a preparation process.
After the microbial fertilizer prepared by the bacterial strain with the indoleacetic acid synthesis function is applied, the microorganism synthesizes the indoleacetic acid by using a substrate, so that on one hand, the environmental pollution in the chemical synthesis process of the regulator can be reduced; on the other hand, microorganisms are easier to colonize on crops, can activate and improve soil to improve the utilization rate of the fertilizer, contains rich nutrients, is easy to be absorbed by plants, ensures the nutrient absorption while promoting cell division by indoleacetic acid, realizes the synergistic action of 'regulator-nutrient', and plays a positive role in stress resistance and yield increase of the crops.
The invention content is as follows:
the invention aims to provide application of bacillus LY152 in preparation of microbial fertilizers.
The invention is realized by the following steps:
application of bacillus LY152 in preparation of microbial fertilizer, wherein bacillus LY152 is named as LatinBacillus sp.And 8.25.8.2014, which is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC NO 9541.
Strain LY152 has a 3-indoleacetic acid producing function which is not obvious or foreseeable from Bacillus LY152 itself. Strain LY152 was inoculated with 50ml of liquid R 2 A medium was placed in a triangular flask and subjected to shake culture at 28 ℃ for 48 hours at 150rpm/min, and 50. Mu.L of the bacterial suspension was dropped onto a white ceramic plate while adding 50. Mu.L of a colorimetric solution (Salkowski colorimetric solution: 50mL35% HClO4 +1mL0.5mol/LFeCl 3) to the bacterial suspension. The white ceramic plate was left at room temperature in the dark for 30 min and then observed for color change. The color changed to pink was positive and indicated that IAA was secreted, and the darker the color was greater the secretion intensity, the color did not change to negative and indicated that IAA was not secreted. The suspension of strain LY152 is dark pink, which shows that the strain has obvious IAA secretion performance. R of the invention 2 The formula of the culture medium A is as follows: 0.5g of yeast extract powder, 0.5g of tryptone, 0.5g of casamino acid, 0.5g of glucose, 0.5g of soluble starch, 0.3g of sodium pyruvate and 0.3g of K 2 HPO4，0.05gMgSO 4 ·7H 2 O,0.5 g of L-tryptophan and distilled water are added until the volume is 1000 mL, and the pH value is 7.2 +/-0.2.
The microbial fertilizer prepared by the preparation method and the application comprises a bacillus LY152 microbial inoculum, nutrient components, additives and fillers. Wherein the nutritional ingredients comprise yeast extract powder, glucose and L-tryptophan; the auxiliary agent is any one or the combination of two of sodium lignosulfonate and sodium dodecyl benzene sulfonate; the filler is one or the combination of two of kaolin, calcium carbonate, diatomite, bentonite and white carbon black. The components are calculated by the following weight percentage: 1-2% of bacillus LY152 microbial inoculum (the effective viable count is more than 100 hundred million cfu/g), 5-10% of yeast extract powder, 2-5% of glucose, 0.1-0.2% of L-tryptophan, 1-10% of auxiliary agent and 72.8-90.2% of filler.
The microbial fertilizer is applied to rooting and growth promotion of crops.
The application of the microbial fertilizer in rooting and growth promotion of crops is characterized in that the crops are rice, wheat, corn, rape, vegetables and fruits, and the microbial fertilizer is applied in the crop planting process, so that the germination, rooting and growth of the crops can be effectively promoted, the using amount of the fertilizer is reduced, and the crop yield is increased.
The invention firstly discovers that the bacillus strain can utilize nutrient components in the fertilizer to secrete the 3-heteroauxin, so that the growth promotion effect of the microbial fertilizer can be improved. The prepared microbial fertilizer is rich in nutrition and is beneficial to microbial growth, colonization and crop absorption. After being diluted, the microbial agent is sprayed or irrigated to roots, so that the microorganisms can quickly propagate and secrete 3-indoleacetic acid to stimulate the growth of crops; in addition, the crop can fully absorb and utilize the nutrients in the fertilizer while growing, so that the organic combination of the regulator and the nutrients is realized, and the quality of agricultural products is improved. The microbial fertilizer has obvious yield increasing effect, can improve soil micro-ecology and enhance the disease resistance of crops.
The specific implementation mode is as follows:
example 1:
the application of the bacillus LY152 in preparing the microbial fertilizer comprises the following specific operations:
1. material and apparatus
Strain: bacillus LY152, bacillus LY152 and Latin name thereofBacillus sp.In 2014, 8, 25 days are preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC NO 9541. The invention patent application of the bacillus is published by the Chinese patent office in 2015, 2 months and 25 days.
Equipment: blending machine, low-temperature airflow type crusher and airflow type crusher
2. Method and steps of operation
The microbial fertilizer mainly comprises a microbial inoculum, nutrient components, an auxiliary agent and a filler.
(1) Preparation of each component:
bacterial agent: preparing bacillus LY152 into bacterial powder with effective viable count more than 100 hundred million cfu/g;
the nutrient components are as follows: yeast extract, glucose and L-tryptophan;
auxiliary agent: any one or two of sodium lignin sulfonate and sodium dodecyl benzene sulfonate;
filling: any one or the combination of two of kaolin, calcium carbonate, diatomite, bentonite and white carbon black.
(2) Crushing: adding the nutrient components, the auxiliary agent and the filler into an airflow type grinder respectively, grinding according to the fineness of 325 meshes, and grinding for later use. Adding the microbial inoculum into a low-temperature jet mill, controlling the grinding temperature below 10 ℃, grinding according to the fineness of 325 meshes, and reserving after grinding.
(3) Uniformly mixing: the components are added into a mixing machine according to the following weight percentage and are mixed uniformly to obtain the uniformly dispersed microbial fertilizer. The weight percentages of the components are as follows: 1-2% of microbial inoculum, 5-10% of yeast extract powder, 2-5% of glucose, 0.1-0.2% of L-tryptophan, 1-10% of auxiliary agent and 72.8-90.2% of filler.
Example 2:
the application of the bacillus LY152 in the preparation of the microbial fertilizer for promoting the growth of crops comprises the following specific operations:
1. materials:
preparing a microbial fertilizer: the same as example 1;
crop: after 5 days of transplanting, pepper seedlings with basically consistent growth vigor grow.
2. Method and steps of operation
(1) The fertilization mode is spraying; the application amount is 0.25g/m 2 Diluting with clear water by 300 times, and uniformly spraying the diluted clear water on pepper seedlings; control treatment (CK) with applied water.
(2) The above 2 processes are performed at 20m per cell area 2 The arrangement was made in triplicate.
(3) After 30 days, the plant height and the root length are measured, and the result shows that after the microbial fertilizer is applied, the plant height is increased by 8-10% compared with a control group, and the root length is increased by 7-9% compared with the control group. The growth of the pepper can be obviously promoted by applying the microbial fertilizer.
Example 3:
the bacillus LY152 is utilized to prepare the microbial fertilizer, and the formula of the microbial fertilizer has a synergistic effect on promoting the growth of crops, and the preparation method specifically comprises the following steps:
1. materials:
preparing a microbial fertilizer: the same as example 1;
crop: and after 5d of transplanting, basically growing the cucumber seedlings with consistent growth vigor.
2. Method and steps of operation
(1) The fertilization mode is spraying; the application amount is 0.25g/m 2 And diluting with clear water by 300 times, and uniformly spraying the diluted clear water on cucumber seedlings.
(2) The test is provided with four treatments, wherein the treatment A is the microbial fertilizer treatment prepared in the example 1, the treatment B is the microbial fertilizer treatment without addition of LY152 fungicide (the rest components are unchanged), the treatment C is the microbial fertilizer treatment without addition of nutrient components (the rest components are unchanged), and the treatment D is clear water control. The four processes are carried out according to the area of each cell of 20m 2 The arrangement was performed in triplicate.
(3) After 30d, the plant height is determined, and the result shows that: in the treatment A, the plant height is increased by 8-11% compared with a control group, and the effect is obvious; in the treatment of B, the plant height is increased by 2-3% compared with a control group, and the effect is not obvious; in the C treatment, the plant height is increased by 1-2% compared with the control group, and the effect is not obvious. The microbial fertilizer has the beneficial effects that the bacterial strains in the formula of the microbial fertilizer are matched with the nutrient components, so that the synergistic effect is realized, and the growth of crops can be remarkably promoted.
The above embodiments are further illustrative of the present invention, but it should not be construed that the scope of the above subject matter is limited to the above embodiments. All the technologies implemented based on the above-mentioned contents belong to the scope of the present invention.
Claims (3)
1. The application of the bacillus LY152 in preparing the microbial fertilizer with the crop rooting and growth promoting effects is characterized in that the bacillus LY152 is deposited in the China general microbiological culture Collection center in 8-25.2014 under the Latin names Bacilus sp, and the preservation number is CGMCC NO 9541: the prepared microbial fertilizer comprises a bacillus LY152 microbial inoculum, nutrient components, an auxiliary agent and a filler, wherein the bacillus LY152 has the function of producing 3-indoleacetic acid; the nutritional ingredients are yeast extract powder, glucose and L-tryptophan; the auxiliary agent is any one or the combination of two of sodium lignosulfonate and sodium dodecyl benzene sulfonate; the filler is any one or the combination of two of kaolin, calcium carbonate, diatomite, bentonite and white carbon black, and the components are calculated by the following weight percentage: the effective viable count of the bacillus LY152 is more than 100 hundred million cfu/g,1-2%, 5-10% of yeast extract powder, 2-5% of glucose, 0.1-0.2% of L-tryptophan, 1-10% of auxiliary agent and 72.8-90.2% of filler.
2. The use of the microbial fertilizer of claim 1 for rooting and promoting growth of crops.
3. The application of the microbial fertilizer in rooting and promoting of crops as claimed in claim 2, wherein the crops are rice, wheat, corn, rape, vegetable and fruit, and the microbial fertilizer is applied in the crop planting process, so that the germination, rooting and growth of crops can be effectively promoted, the fertilizer usage amount is reduced, and the crop yield is increased.
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| KR20060000730A (en) * | 2004-06-29 | 2006-01-06 | 강원대학교산학협력단 | Microbial fertilizer containing the mixture of pseudomonas fluorescens mc07 and bacillus megaterium |
| CN102049412A (en) * | 2009-11-05 | 2011-05-11 | 南开大学 | Biological compound preparation for repairing soil heavy metal pollution |
| CN102424626A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof |
| CN102580999B (en) * | 2012-03-02 | 2016-06-08 | 北京丹路实业公司 | A kind of agricultural microbial agent control heavy metal pollution of soil farm crop technology |
| CN103820348B (en) * | 2012-11-16 | 2016-03-02 | 中国科学院生态环境研究中心 | The production method of one strain plant growth-promoting bacteria and microbial inoculum thereof and application |
| CN103275895B (en) * | 2013-05-24 | 2014-10-08 | 南京农业大学 | Saline-alkali-tolerant heteroauxin-producing Bacillus subtilis and application thereof |
| CN103275728B (en) * | 2013-06-24 | 2015-06-03 | 东莞市保得生物工程有限公司 | Soil heavy metal biological fixing agent and application method thereof |
| CN103408334B (en) * | 2013-07-30 | 2015-12-09 | 湖北中化东方肥料有限公司 | A kind of can the bio-organic fertilizer and preparation method thereof of passivation heavy metal-polluted soil |
| CN103992963B (en) * | 2014-03-19 | 2016-06-22 | 南京博农生物科技有限公司 | A kind of bacillus megaterium and application thereof |
| CN103952350B (en) * | 2014-04-24 | 2016-05-18 | 烟台地元生物科技有限公司 | Spindle lysine bacillus and microbial bacterial agent and their application |
| CN104195070B (en) * | 2014-08-06 | 2016-07-06 | 湖南省微生物研究院 | A kind of reduce the bacterial strain of cadmium content, microbial inoculum and preparation and application thereof |
| CN104195074B (en) * | 2014-08-13 | 2016-10-19 | 甘肃农业大学 | A kind of deep brown Bacillus strain of Alpine Grasslands herbage endogenetic bacteria, microbial bacterial agent and its preparation method and application |
| CN104109053B (en) * | 2014-08-14 | 2016-05-04 | 杨晓辉 | A kind of microbial manure that falls agricultural chemicals and heavy-metal residual and preparation method thereof |
| CN104371956B (en) * | 2014-11-14 | 2017-07-28 | 四川省兰月科技有限公司 | There is bacillus and the purposes of blocking effect to cadmium |
| CN104844284B (en) * | 2015-03-31 | 2017-08-11 | 山东宝来利来生物工程股份有限公司 | A kind of rhizosphere growth-promoting type water-soluble vitamins bacterial manure |
| CN104928212B (en) * | 2015-06-03 | 2018-04-27 | 华南农业大学 | Bacillus megaterium X3 and preparation method thereof, application |
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