CN106007950A - Application of bacillus LY152 to preparing microbial fertilizers - Google Patents

Application of bacillus LY152 to preparing microbial fertilizers Download PDF

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Publication number
CN106007950A
CN106007950A CN201610339599.0A CN201610339599A CN106007950A CN 106007950 A CN106007950 A CN 106007950A CN 201610339599 A CN201610339599 A CN 201610339599A CN 106007950 A CN106007950 A CN 106007950A
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bacillus
microbial
application
microbial manure
bacillus cereus
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CN201610339599.0A
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CN106007950B (en
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王强锋
侯勇
朱彭玲
夏中梅
王海涛
王赟
曾芸
曾显斌
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Sichuan Lanyue Science & Technology Co Ltd
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Sichuan Lanyue Science & Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C11/00—Other nitrogenous fertilisers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Soil Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses application of bacillus LY152 to preparing microbial fertilizers. The fact that bacillus strains can secrete 3-indoleacetic acid by the aid of nutritional components in the fertilizers is discovered at first. The Latin name of the bacillus LY152 is Bacillus sp., the bacillus LY152 is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on 25 August, 2014, and a preservation number of the bacillus LY152 is CGMCC NO 9541. The microbial fertilizers prepared from the bacillus LY152 comprise bacillus LY152 microbial agents, the nutritional components, auxiliaries and fillers. The application has the advantages that the microbial fertilizers contain abundant nutrition and are favorable for microbial growth and colonization and crop absorption; microorganisms can quickly breed and secrete the 3-indoleacetic acid, accordingly, crops can be stimulated to grow and can sufficiently absorb nutrients in the fertilizers, obvious yield increasing effects can be realized, the microbial ecology of soil can be improved, and the disease resistance of the crops can be strengthened.

Description

Bacillus cereus LY152 In the application prepared on microbial manure
Technical field:
The present invention relates to microbial manure preparation field, the bacterial strain particularly relating to product heteroauxing prepares method and the application of microbial manure.
Background technology:
The population in the Liao Zhan world 22% is brought up to account for ploughing of the world 7% by China, and the use of chemical fertilizer is performed meritorious deeds never to be obliterated.Chemical fertilizer is important capital goods in the agricultural sector.Chemical fertilizer has played irreplaceable effect in promoting grain and Agriculture Production, but currently also there is the problems such as chemical fertilizer excess is used, blindness is used, and brings the increase of cost and the pollution of environment.It is increasingly faster that " action of fertilizer application amount zero growth rate " that relevant departments implement makes traditional agriculture convert to the ecological agriculture, and the appearance of the ecological agriculture has expedited the emergence of exploitation and the application of microbial manure.Microbial manure is nontoxic, free from environmental pollution, can be movable by the vital metabolic of microorganism, increase the nitrogen in soil or available phosphorus, the content of potassium, or scalable crop hormone substance level, or the activity of Suppressing phytopathogens, thus increase soil fertility, improve crop alimentary condition and growing environment, improve crop yield.The usage amount of chemical fertilizer can be reduced by the use of microbial manure, thus effectively alleviate the deterioration of ecological environment of soil.
Therefore, seeking use high-effective microorganism fertilizer, improve chemical fertilizer utilization ratio, reduce the usage amount of chemical fertilizer and pesticide, the sustainable development of agricultural of improving the ecological environment, realize, is the agriculture urgent task of current China.Although having some microbial manures in the market, such as: azotobacteria fertilizer, phosphorus additives, B. mucilaginocus are fertile.These fertilizer also exist that consumption is big, high in cost of production problem, but also there is the problem that some application effect of products are unstable.
Heteroauxing (indole-3-acetic acid) is the main active of natural plants auxin, many processes of involved in plant development.Microorganism can promote plant growing by secretion heteroauxing.IAA can increase size and the distribution of plant roots, beneficially root and absorb more nutrient substance from soil.Producing phytohormone is one of microorganism main mechanism with host plant interaction, and bacterial strain is easily grown surely plant, plays a role.Therefore, utilize the bacterial strain with secretion 3-indolyl acetic acid function, add other components, design preparation flow and technique, produce novel microbial manure significant.
Utilizing and have microbial manure prepared by the bacterial strain of heteroauxing complex functionality, after using, microorganism utilizes substrate synthesis of indole acetic acid, on the one hand, can reduce the environmental pollution of regulator chemosynthesis process；On the other hand, microorganism is easier to surely grow crop, improvement soil can be activated and improve utilization rate of fertilizer, and containing abundant nutrient substance in microbial manure, easily it is absorbed by plants, while heteroauxing promotes cell division, ensure Nutrient Absorption, it is achieved the synergism of " regulator-nutrient ", crop reverse-resisting yield-increasing is had a positive effect.
Summary of the invention:
It is an object of the invention to provide bacillus cereus LY152 and prepare the application of microbial manure.
The present invention is achieved in that
Bacillus cereus LY152 is in the application prepared on microbial manure, its Latin literary fame of described bacillus cereus LY152Bacillus sp., it being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on August 25th, 2014, preserving number is CGMCC NO 9541.
Bacterial strain LY152 has product 3-indolyl acetic acid function, and this function can not the apparent or prediction from bacillus cereus LY152 itself.Bacterial strain LY152 has been inoculated in 50ml liquid R2In the triangular flask of A culture medium, it is placed in 28 DEG C of shaking tables to cultivate, rotating speed is 150rpm/min, after cultivating 48h, take its bacteria suspension 50 μ L to drip and be placed on whiteware plate, add 50 μ L color solutions (Salkowski color solution: 50mL35%HClO4+1mL0.5mol/LFeCl3) simultaneously in this bacteria suspension.Whiteware plate is put and after lucifuge under room temperature places 30 min, observes the change of its color.Color becomes pink person for the positive, expression can producing IAA, color more deeply feels and shows that the intensity of secretion is the biggest, do not change color as feminine gender, and expression can not producing IAA.Bacterial strain LY152 bacteria suspension color is rediance, shows have significant IAA secernment property.The R of the present invention2A culture medium prescription is as follows: 0.5g yeast leaching powder, 0. 5g tryptone, 0. 5g casamino acid, 0.5g glucose, 0.5g soluble starch, 0.3g Sodium Pyruvate, 0.3gK2HPO4,0.05gMgSO4·7H2O, 0.5 g L-Trp, distilled water is settled to 1000 mL, and pH is 7.2 ± 0.2.
The preparation method of described microbial manure and application, the microbial manure of preparation includes bacillus cereus LY152 microbial inoculum, nutritional labeling, auxiliary agent and filler.Wherein, nutritional labeling is yeast leaching powder, glucose and L-Trp；Auxiliary agent is any one or the combination of two kinds in sodium lignin sulfonate and dodecylbenzene sodium sulfonate；Filler is any one or the combination of two kinds in Kaolin, calcium carbonate, kieselguhr, bentonite, white carbon.Each component is by following percentage by weight meter: and bacillus cereus LY152 microbial inoculum (living bacteria count > 10,000,000,000 cfu/g) 1-2%, yeast leaching powder 5%-10%, glucose 2-5%, L-Trp 0.1-0.2%, auxiliary agent 1%-10%, filler 72.8%-90.2%.
Described microbial manure is taken root the application in growth promoting crops.
Described microbial manure is taken root the application in growth promoting crops, it is characterized in that described crops are Oryza sativa L., Semen Tritici aestivi, Semen Maydis, Brassica campestris L, veterinary antibiotics, this microbial manure is used during proportion of crop planting, crop can be effectively facilitated germinate, take root and grow, reduce fertilizer application amount, increase crop yield.
First the present invention finds that Bacillus strain can utilize fertilizer Middle nutrition composition to secrete 3-indolyl acetic acid, can increase microbial manure growth-promoting effect.Prepared microbial manure is nutritious, and beneficially growth of microorganism is grown and Crop surely.Spraying or pouring root after being diluted, microorganism energy Fast-propagation is also secreted 3-indolyl acetic acid, is stimulated plant growth；Further, crop can fully absorb, while growth, the nutrient utilized in this fertilizer, it is achieved " regulator and the combination of nutrient ", improves quality of agricultural product.Described microbial manure obvious effect of increasing production, can improve soil micro-ecosystem, strengthens crop disease-resistant ability.
Detailed description of the invention:
Embodiment 1 :
Bacillus cereus LY152 is in the application prepared on microbial manure, and concrete operations are as follows:
1, material and equipment
Bacterial strain: bacillus cereus LY152, its Latin literary fame of bacillus cereus LY152Bacillus sp., it being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on August 25th, 2014, preserving number is CGMCC NO 9541.The application for a patent for invention of this bacillus cereus is announced by Patent Office of the People's Republic of China on February 25th, 2015.
Equipment: mixing machine, low-temperature airflow formula pulverizer, jet mill
2, operational approach and step
Described microbial manure main component includes microbial inoculum, nutritional labeling, auxiliary agent and filler.
(1) preparation of each component:
Microbial inoculum: bacillus cereus LY152 is made the living bacteria count mycopowder more than 10,000,000,000 cfu/g；
Nutritional labeling: yeast leaching powder, glucose and L-Trp；
Auxiliary agent: any one or the combination of two kinds in sodium lignin sulfonate and dodecylbenzene sodium sulfonate；
Filler: any one or the combination of two kinds in Kaolin, calcium carbonate, kieselguhr, bentonite, white carbon.
(2) pulverize: nutritional labeling, auxiliary agent, filler are added separately in jet mill, by 325 mesh finess disintegratings, standby after having pulverized.Being joined by microbial inoculum in low-temperature airflow pulverizer, control pulverizing temperature is below 10 DEG C, by 325 mesh finess disintegratings, standby after having pulverized.
(3) mixing: each component adds in mixing machine by following percentage by weight, mix homogeneously obtains homodisperse microbial manure.Each weight percentages of components is: microbial inoculum 1-2%, yeast leaching powder 5%-10%, glucose 2-5%, L-Trp 0.1-0.2%, auxiliary agent 1%-10%, filler 72.8%-90.2%.
Embodiment 2 :
Utilizing bacillus cereus LY152 promoting the application in plant growth at the microbial manure of preparation, concrete operations are as follows:
1, material:
Prepared by microbial manure: with embodiment 1；
Crop: after transplanting 5d, the Hot Pepper Seedling that growing way is basically identical.
2, operational approach and step
(1) fertilization mode is spraying；Amount of application is 0.25g/m2, clear water uniformly sprays application on Hot Pepper Seedling after diluting 300 times；To execute clear water as control treatment (CK).
(2) more than, 2 process by every plot area 20m2Arrangement, repeats for three times.
(3) measuring plant height, root length after 30d, result shows, after using microbial manure, plant height increases 8-10% than matched group, and root length increases 7-9% than matched group.Use microbial manure process and can remarkably promote chili growth.
Embodiment 3 :
Utilizing bacillus cereus LY152 promoting potentiation in plant growth at its formula of microbial manure of preparation, concrete operations are as follows:
1, material:
Prepared by microbial manure: with embodiment 1；
Crop: after transplanting 5d, the cucumber seedling that growing way is basically identical.
2, operational approach and step
(1) fertilization mode is spraying；Amount of application is 0.25g/m2, clear water uniformly sprays application on cucumber seedling after diluting 300 times.
(2) test arranges four process altogether, A is processed as the microbial manure prepared by embodiment 1 and processes, B is processed as the described microbial manure without LY152 microbial inoculum and processes (remaining component is constant), C is processed as processing (remaining component is constant) without the microbial manure of nutritional labeling, and D is processed as clear water comparison.Above four process by every plot area 20m2Arrangement, repeats for three times.
(3) measuring plant height after 30d, result shows: during A processes, plant height increases 8-11% than matched group, and effect is notable；During B processes, plant height increases 2-3% than matched group, and effect is the most notable；During C processes, plant height increases 1-2% than matched group, and effect is the most notable.Illustrate that in this microbe fertilizer formulation, bacterial strain has potentiation with nutritional labeling collocation, can remarkably promote plant growth.
Above-described embodiment is that the foregoing to the present invention is further described, but this should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to above-described embodiment.All technology realized based on foregoing belong to the scope of the present invention.

Claims (4)

1. bacillus cereus LY152 is in the application prepared on microbial manure, described bacillus cereus LY152, its Latin literary fameBacillus sp., it being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on August 25th, 2014, preserving number is CGMCC NO 9541。
Bacillus cereus LY152 the most according to claim 1 is in the application prepared on microbial manure, it is characterized in that: the microbial manure of preparation includes bacillus cereus LY152 microbial inoculum, nutritional labeling, auxiliary agent and filler, bacillus cereus LY152 has product 3-indolyl acetic acid function；Nutritional labeling is yeast leaching powder, glucose and L-Trp；Auxiliary agent is any one or the combination of two kinds in sodium lignin sulfonate and dodecylbenzene sodium sulfonate；Filler is any one or the combination of two kinds in Kaolin, calcium carbonate, kieselguhr, bentonite, white carbon, each component is by following percentage by weight meter: bacillus cereus LY152(living bacteria count > 10,000,000,000 cfu/g) 1-2%, yeast leaching powder 5%-10%, glucose 2-5%, L-Trp 0.1-0.2%, auxiliary agent 1%-10%, filler 72.8%-90.2%.
Microbial manure the most according to claim 2 is taken root the application in growth promoting crops.
Microbial manure the most according to claim 3 is taken root the application in growth promoting crops, it is characterized in that described crops are Oryza sativa L., Semen Tritici aestivi, Semen Maydis, Brassica campestris L, veterinary antibiotics, this microbial manure is used during proportion of crop planting, crop can be effectively facilitated germinate, take root and grow, reduce fertilizer application amount, increase crop yield.
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Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060000730A (en) * 2004-06-29 2006-01-06 강원대학교산학협력단 Microbial fertilizer containing the mixture of pseudomonas fluorescens mc07 and bacillus megaterium
CN102049412A (en) * 2009-11-05 2011-05-11 南开大学 Biological compound preparation for repairing soil heavy metal pollution
CN102424626A (en) * 2011-09-05 2012-04-25 麻林涛 Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof
CN102580999A (en) * 2012-03-02 2012-07-18 北京丹路实业公司 Technology for utilizing agricultural microbial agent to prevent and treat crops polluted by soil heavy metal
CN103275728A (en) * 2013-06-24 2013-09-04 东莞市保得生物工程有限公司 Soil heavy metal biological fixing agent and application method thereof
CN103275895A (en) * 2013-05-24 2013-09-04 南京农业大学 Saline-alkali-tolerant heteroauxin-producing Bacillus subtilis and application thereof
CN103408334A (en) * 2013-07-30 2013-11-27 湖北中化东方肥料有限公司 Biological-organic fertilizer capable of passivating soil heavy metals and preparation method thereof
CN103820348A (en) * 2012-11-16 2014-05-28 中国科学院生态环境研究中心 Plant growth-promoting bacteria and preparation method and application of fungicide
CN103952350A (en) * 2014-04-24 2014-07-30 烟台地元生物科技有限公司 Lysinibacillus fusiformis and microbial agent, as well as applications thereof
CN103992963A (en) * 2014-03-19 2014-08-20 南京博农生物科技有限公司 Bacillus megaterium and application thereof
CN104109053A (en) * 2014-08-14 2014-10-22 杨晓辉 Microbial fertilizer for reducing residues of pesticides and heavy metals and preparation method of microbial fertilizer
CN104195070A (en) * 2014-08-06 2014-12-10 湖南省微生物研究院 Strain and microbial agent capable of reducing content of cadmium as well as preparation and application methods of strain and microbial agent
CN104195074A (en) * 2014-08-13 2014-12-10 甘肃农业大学 Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent
CN104371956A (en) * 2014-11-14 2015-02-25 四川省兰月科技有限公司 Bacillus with blocking effect on cadmium and application of bacillus
CN104844284A (en) * 2015-03-31 2015-08-19 山东宝来利来生物工程股份有限公司 Rhizosphere growth-promoting water-soluble microbial bacterium manure
CN104928212A (en) * 2015-06-03 2015-09-23 华南农业大学 Bacillus megaterium strain X3 and preparation method and application thereof

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060000730A (en) * 2004-06-29 2006-01-06 강원대학교산학협력단 Microbial fertilizer containing the mixture of pseudomonas fluorescens mc07 and bacillus megaterium
CN102049412A (en) * 2009-11-05 2011-05-11 南开大学 Biological compound preparation for repairing soil heavy metal pollution
CN102424626A (en) * 2011-09-05 2012-04-25 麻林涛 Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof
CN102580999A (en) * 2012-03-02 2012-07-18 北京丹路实业公司 Technology for utilizing agricultural microbial agent to prevent and treat crops polluted by soil heavy metal
CN103820348A (en) * 2012-11-16 2014-05-28 中国科学院生态环境研究中心 Plant growth-promoting bacteria and preparation method and application of fungicide
CN103275895A (en) * 2013-05-24 2013-09-04 南京农业大学 Saline-alkali-tolerant heteroauxin-producing Bacillus subtilis and application thereof
CN103275728A (en) * 2013-06-24 2013-09-04 东莞市保得生物工程有限公司 Soil heavy metal biological fixing agent and application method thereof
CN103408334A (en) * 2013-07-30 2013-11-27 湖北中化东方肥料有限公司 Biological-organic fertilizer capable of passivating soil heavy metals and preparation method thereof
CN103992963A (en) * 2014-03-19 2014-08-20 南京博农生物科技有限公司 Bacillus megaterium and application thereof
CN103952350A (en) * 2014-04-24 2014-07-30 烟台地元生物科技有限公司 Lysinibacillus fusiformis and microbial agent, as well as applications thereof
CN104195070A (en) * 2014-08-06 2014-12-10 湖南省微生物研究院 Strain and microbial agent capable of reducing content of cadmium as well as preparation and application methods of strain and microbial agent
CN104195074A (en) * 2014-08-13 2014-12-10 甘肃农业大学 Endophytic bacteria bacillus atrophaeus bacterial strain of forage in alpine grassland, microbial agent as well as preparation method and application of microbial agent
CN104109053A (en) * 2014-08-14 2014-10-22 杨晓辉 Microbial fertilizer for reducing residues of pesticides and heavy metals and preparation method of microbial fertilizer
CN104371956A (en) * 2014-11-14 2015-02-25 四川省兰月科技有限公司 Bacillus with blocking effect on cadmium and application of bacillus
CN104844284A (en) * 2015-03-31 2015-08-19 山东宝来利来生物工程股份有限公司 Rhizosphere growth-promoting water-soluble microbial bacterium manure
CN104928212A (en) * 2015-06-03 2015-09-23 华南农业大学 Bacillus megaterium strain X3 and preparation method and application thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张颖等: "《土壤污染与防治》", 31 October 2012, 中国林业出版社 *
王正银: "《肥料研制与加工》", 31 December 2015, 中国农业大学出版社 *

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