CN105996025A - Preparing method for rhodiolae root fermentation liquor - Google Patents
Preparing method for rhodiolae root fermentation liquor Download PDFInfo
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- CN105996025A CN105996025A CN201610309209.5A CN201610309209A CN105996025A CN 105996025 A CN105996025 A CN 105996025A CN 201610309209 A CN201610309209 A CN 201610309209A CN 105996025 A CN105996025 A CN 105996025A
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- radix rhodiolae
- water
- food
- weight
- fermentation
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- 230000004151 fermentation Effects 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title abstract description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 66
- 235000013305 food Nutrition 0.000 claims abstract description 59
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 54
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 44
- 239000004310 lactic acid Substances 0.000 claims abstract description 27
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 27
- 241000894006 Bacteria Species 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 24
- 239000011780 sodium chloride Substances 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims abstract description 18
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- 239000000284 extract Substances 0.000 claims abstract description 4
- 244000179886 Moringa oleifera Species 0.000 claims abstract 3
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- 238000002360 preparation method Methods 0.000 claims description 31
- 239000002253 acid Substances 0.000 claims description 25
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a preparing method for rhodiolae root fermentation liquor. The preparing method comprises the following steps that firstly, food enzymatic hydrolysate is prepared; secondly, a rhodiolae root extract solution is prepared; thirdly, a rhodiolae root nutrient solution is prepared; fourthly, fermentation is carried out; fifthly, emulsification is carried out. According to the preparing method, rhodiolae roots, moringa oleifera powder and various medicinal and edible materials are matched in a reasonable proportion and sufficiently mixed, and then lactic acid bacteria are added for fermentation, wherein in the fermentation process, the single culture lactic acid bacteria are adopted, a specially-made culture solution serves as a hotbed, the fermentation efficiency of lactic acid bacteria is more sufficiently exerted, and it is ensured that the pH value of the solution is maintained within a pre-controlled range in the fermentation process by using the combination of sodium chloride and acetic acid as a buffering solution.
Description
Technical field
The invention belongs to health protection medicine field, relate to fermentation technique, the preparation method of a kind of Radix Rhodiolae fermentation liquid.
Background technology
Radix Rhodiolae is dry root and the rhizome of crassulaceae plants Radix Rhodiolae, and effect of Radix Rhodiolae and effect are energy the kidney invigorating, regulate the flow of vital energy
Nourishing blood, have promoting blood circulation and hemostasis, clearing away lung-heat to relieve cough, antipyretic, and the effect under leukorrhagia stopping, the side effect of Radix Rhodiolae does not the most find.Red
Herba hylotelephii erythrosticti can improve sleep, detumescence is become silted up in blood-enriching and blood-promoting circulation, anti-cerebral anoxia, resisting fatigue, promoting blood circulation and hemostasis, clearing away lung-heat to relieve cough, change, antipyretic move back
Burn, nourish the effects such as vigour；It is used for outward treating traumatic injury and burn and scald.The applicating history of Radix Rhodiolae is long, before more than 2,000 year,
The people of Qinghai-Tibet Platean is just used as medicine with it, with building body, the impact of opposing poor environment.Among the people being commonly used to is decocted water or steeps in wine, with
Banish fatigue or resist mountain area cold.Also diseases prevention is healthy and strong and nourishing lengthens one's life simultaneously.Because it has strengthening healthy qi, benefiting qi and nourishing blood, YIN nourishing
Magical effect of lung benefiting, successive dynasties Tibetan medicine is regarded as " lucky three essentials-essence ".Human body oxygen content is not enough, arises that hypoxic conditions,
Including headache, tired, dyspnea, dizziness etc., serious or paroxysmal, can cause that stupor is even dead.In general,
This phenomenon often betides highlands, causes high mountain disease, even some fatal complication.Anoxia is a kind of stressor,
Health can be caused to produce a series of stress.The adaptive significances of Radix Rhodiolae, can promote non-specific to destructive stimulus of human body
Property resistance, strengthen human body adaptability.Oxygen through reinforcement inner cell spreads, or oxygen uses benefit, antioxidative
Ability, helps and improves the adaptive faculty that anoxia is stimulated by health.
Radix Rhodiolae is the most only used to medicine or medicated diet, and applicant does not the most find to be used for the form of fermentation liquid
The report of daily life and technical literature.
Summary of the invention
It is an object of the invention to overcome prior art not enough, it is provided that a kind of Radix Rhodiolae improving body activity and metabolic rate
The preparation method of fermentation liquid.
The technical solution used in the present invention is:
The preparation method of a kind of Radix Rhodiolae fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:5, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, after 80 DEG C of 15min that precook, add and adjust the total of pH value 5.5, addition food and water with citric acid
The 0.02% pectase 55 DEG C insulation enzymolysis 2h of weight, adds 1% hemicellulase of the gross weight of food and water, adds food
With 0.56% cellulase of the gross weight of water, add 0.5% papain of the gross weight of food and water, add food and water
0.04% phytase 1h of gross weight, mix Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filter, sterilization, obtain food enzymolysis solution, stand-by；
(2) preparing Radix Rhodiolae extracting solution: by Radix Rhodiolae water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds water and beats
Slurry, then mixes with moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, Radix Raphani, adds water to 2.5 times of the initial water yield, decocts, extraction
Supernatant, obtains Radix Rhodiolae extracting solution, stand-by；
(3) the preparation of Radix Rhodiolae nutritional solution: food enzymolysis solution is mixed by weight the proportions for 4:1 with Radix Rhodiolae extracting solution,
Mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Radix Rhodiolae nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Radix Rhodiolae nutritional solution, Radix Rhodiolae nutritional solution and culture fluid weight ratio are 10:1, add breast
Acid bacterium, sodium chloride and acetic acid mixed fermentation 1day, add above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight lactic acid bacteria,
Sodium chloride and acetic fermentation, obtain fermentation liquid；
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
And, the content of the solid content of the food enzymolysis solution that step is (1) described is 8%～10%.
And, step (2) described in the solid content of Radix Rhodiolae extracting solution be 4%～6%.
And, step (2) described in Radix Rhodiolae, moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, the parts by weight of Semen Raphani are respectively as follows:
20～25 parts, 5～10 parts, 1～2 part, 1～2 part, 1～2 part.
And, the (3) described lactic acid bacteria of step, sodium chloride, the parts by weight of total consumption of acetic acid are respectively 100～120 parts, 8～10
Part, acetic acid is 4～6 parts.
And, the preparation method of the culture fluid that step is (3) described is: maltose is soluble in water, puts into compound after standing 20min
Vitamin, the post-heating that stirs boils, and is cooled to after room temperature add Ovum Gallus domesticus album, stirs, stand-by, maltose, water, multiple
Close vitamin, the weight ratio of Ovum Gallus domesticus album is 100:10000:3:10.
Advantage of the present invention and good effect be:
The present invention is by the collocation by rational proportion of the various materials of Radix Rhodiolae, moringa powder and integration of edible and medicinal herbs, first that food is corresponding
Enzymolysis, is then sufficiently mixed with extracting solution, adds lactic acid bacteria and ferments, and uses single culture in sweat, i.e. breast
Acid bacterium, using special culture fluid as hotbed, its fermentation efficiency plays more abundant, and the combination of sodium chloride and acetic acid is as slow
Dissolved liquid ensure that in sweat, solution ph is maintained in the range of pre-control.
In the present invention, the consumption of each component is through test of many times and the most constantly to contrast, it was found that involved in technical scheme
Amount ranges can produce unexpected effect, by effective fermentation, make the battalion that Radix Rhodiolae, moringa powder and Radix Glycyrrhizae are main
Foster effect is doubled, although the consumption of each component and effect thereof are only to embody with word and numerical value, but its operating procedure overcomes
Many technology prejudice of the art, is just improved skin activity and has the Radix Rhodiolae fermentation liquid of blood circulation promoting and blood stasis dispelling function.
Detailed description of the invention:
Below by specific embodiment, the invention will be further described, and following example are illustrative, is not determinate,
Protection scope of the present invention can not be limited with this.
Embodiment 1
The preparation method of a kind of Radix Rhodiolae fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:5, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, it is allowed to easily to boil, removes raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust with citric acid
PH value 5.5, adds the 0.02% pectase 55 DEG C insulation enzymolysis 2h of the gross weight of food and water, adds food and the gross weight of water
1% hemicellulase, add 0.56% cellulase of the gross weight of food and water, add food and water gross weight 0.5%
0.04% phytase 1h of the gross weight of papain, addition food and water, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, sterilization,
Stand-by (solid content 8%～10%)；
(2) preparing Radix Rhodiolae extracting solution: by Radix Rhodiolae water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds water and beats
Slurry, then mixes with moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield, decocts, takes out
Taking supernatant, stand-by (solid content 4%～6%), Radix Rhodiolae, moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, the weight of Semen Raphani are divided
It is not: 2000g, 500g, 100g, 100g, 100g, 100g；
(3) the preparation of Radix Rhodiolae nutritional solution: food enzymolysis solution is mixed by weight the proportions for 4:1 with Radix Rhodiolae extracting solution,
Mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Radix Rhodiolae nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Radix Rhodiolae nutritional solution, Radix Rhodiolae nutritional solution and culture fluid weight ratio are 10:1, add breast
Acid bacterium, sodium chloride and acetic acid mixed fermentation 1day, add above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight lactic acid bacteria,
Sodium chloride and acetic fermentation, obtain fermentation liquid, and lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid are respectively 120g, chlorination
Sodium is 9g, and acetic acid is 4.5g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%,
Citric acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylose
Alcohol, 0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl are fine
Dimension element sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5%
Ethyl vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%),
Whole or a portion in 0.06% daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter →
Sterilizing (mixed liquor being put in autoclave, be heated to 90 DEG C, and keep 5m in) → directly beverage can be administered orally, then by Radix Rhodiolae
Need not step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all that each material extracts with food enzymolysis solution and Radix Rhodiolae
The percentage ratio of the mixed gross weight of liquid.
Embodiment 2
The preparation method of a kind of Radix Rhodiolae fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:5, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, it is allowed to easily to boil, removes raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust with citric acid
PH value 5.5, adds the 0.02% pectase 55 DEG C insulation enzymolysis 2h of the gross weight of food and water, adds food and the gross weight of water
1% hemicellulase, add 0.56% cellulase of the gross weight of food and water, add food and water gross weight 0.5%
0.04% phytase 1h of the gross weight of papain, addition food and water, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, sterilization,
Stand-by (solid content 8%～10%)；
(2) preparing Radix Rhodiolae extracting solution: by Radix Rhodiolae water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds water and beats
Slurry, then mixes with moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield, decocts, takes out
Taking supernatant, stand-by (solid content 4%～6%), Radix Rhodiolae, moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, the weight of Semen Raphani are divided
It is not: 2500g, 1000g, 150g, 150g, 150g, 150g；
(3) the preparation of Radix Rhodiolae nutritional solution: food enzymolysis solution is mixed by weight the proportions for 4:1 with Radix Rhodiolae extracting solution,
Mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Radix Rhodiolae nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Radix Rhodiolae nutritional solution, Radix Rhodiolae nutritional solution and culture fluid weight ratio are 10:1, add breast
Acid bacterium, sodium chloride and acetic acid mixed fermentation 1day, add above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight lactic acid bacteria,
Sodium chloride and acetic fermentation, obtain fermentation liquid, and lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid are respectively 120g, chlorination
Sodium is 9g, and acetic acid is 6g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%,
Citric acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylose
Alcohol, 0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl are fine
Dimension element sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5%
Ethyl vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%),
Whole or a portion in 0.06% daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter →
Sterilizing (mixed liquor being put in autoclave, be heated to 90 DEG C, and keep 5m in) → directly beverage can be administered orally, then by Radix Rhodiolae
Need not step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all that each material extracts with food enzymolysis solution and Radix Rhodiolae
The percentage ratio of the mixed gross weight of liquid.
Embodiment 3
The preparation method of a kind of Radix Rhodiolae fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:5, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, it is allowed to easily to boil, removes raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust with citric acid
PH value 5.5, adds the 0.02% pectase 55 DEG C insulation enzymolysis 2h of the gross weight of food and water, adds food and the gross weight of water
1% hemicellulase, add 0.56% cellulase of the gross weight of food and water, add food and water gross weight 0.5%
0.04% phytase 1h of the gross weight of papain, addition food and water, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, sterilization,
Stand-by (solid content 8%～10%)；
(2) preparing Radix Rhodiolae extracting solution: by Radix Rhodiolae water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds water and beats
Slurry, then mixes with moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, Radix Raphani, adds water to 2.5 times of the initial water yield, decocts, extraction
Supernatant, stand-by (solid content 4%～6%), Radix Rhodiolae, moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, the weight difference of Semen Raphani
For: 2200g, 1100g, 150g, 120g, 150g, 110g；
(3) the preparation of Radix Rhodiolae nutritional solution: food enzymolysis solution is mixed by weight the proportions for 4:1 with Radix Rhodiolae extracting solution,
Mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Radix Rhodiolae nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Radix Rhodiolae nutritional solution, Radix Rhodiolae nutritional solution and culture fluid weight ratio are 10:1, add breast
Acid bacterium, sodium chloride and acetic acid mixed fermentation 1day, add above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight lactic acid bacteria,
Sodium chloride and acetic fermentation, obtain fermentation liquid, and lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid are respectively 120g, chlorination
Sodium is 9g, and acetic acid is 6g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%, Fructus Citri Limoniae
Acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylitol,
0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl cellulose
Sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5% ethyl
Vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%), 0.06%
Whole or a portion in daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter → sterilizing (will
Mixed liquor is put in autoclave, is heated to 90 DEG C, and keeps 5min) → directly beverage can be administered orally by Radix Rhodiolae, then need not step
The most (4) fermentation and step emulsifying (5), above-mentioned percentage ratio is all after each material mixes with food enzymolysis solution and Radix Rhodiolae extracting solution
The percentage ratio of gross weight.
Control experiment
Select the healthy male mice 30 with area, body weight 20 ± 2g, be divided into 3 groups at random: matched group (being labeled as A),
Radix Rhodiolae fermentation liquid group (being labeled as B) and Radix Rhodiolae group (being labeled as C),
The distilled water of A group employing 0.02ml/ (kg d), continuous gavage 10d,
The Radix Rhodiolae fermentation liquid that the embodiment of the present invention 1 of B group employing 2.6g/ (kg d) is given, continuous gavage 10d,
The Radix Rhodiolae distilled water solution of C group employing 2.6g/ (kg d), continuous gavage 10d,
Adaptability swimming secondary, each 10min is carried out during gavage.
Swimming with a load attached to the body is tested
After last gavage 30min, 3 treated animals all randomly select 5, and the mice of root of the tail portion load 5% body weight sheet lead is placed in trip
Swimming case went swimming.The depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, and record mice swimming time, i.e. mice start from swimming
Upstream face 10s is not had without the time come back to mouth and nose.
Fatigue be body under the conditions of certain environment, because of for a long time the heaviest, the most nervous work (including mental and physical)
A kind of physiological phenomenon of the temporary obvious reduction of work efficiency caused.Fatigue is the result of multifactor effect, the most directly
It is the decline of exercise tolerance with objectively performance, and swimming time is all the time by the important indicator as reflection exercise tolerance.
The mensuration of blood lactase acid
After last medication 30min, often organize remaining 5 animals and gather mouse tail blood immediately, measure blood lactase acid I；Then exist
Adopt tail blood after swimming trunk went swimming (not bearing a heavy burden) 20min immediately, measure blood lactase acid II；After rest 40min, again adopt tail blood,
Measure blood lactase acid III.Assay method is carried out according to the explanation of full blood lactic test kit.
The generation of blood lactase acid and the mensuration of degradation rate
The generating rate of blood lactase acid=(rest value before blood lactase acid peak-motion)/move to time × 100% used by peak；Blood breast
Acid degradation speed=(blood lactase acid value after blood lactase acid peak-rest 40min)/40min × 100%.
Statistical analysis
Data acquisition SPSS11.5 software processes, each experimental group uses single factor test methods analyst to compare two-by-two.
Experimental result
The impact on mice heavy burden swimming time of the Radix Rhodiolae fermentation liquid
Radix Rhodiolae fermentation liquid the results are shown in Table 1 to the impact of mice heavy burden swimming time.As shown in Table 1, B group mice negative G
Exhaust swimming time and dramatically increase (P ＜ 0.05) than A group and C group, show that Radix Rhodiolae fermentation liquid has obvious anti-fatigue effect.
Table 1 Radix Rhodiolae fermentation liquid on the impact of mice heavy burden swimming time (min)
Originally test result indicate that: mice swimming time is had no significant effect by A and the C group that this institute is selected, and Radix Rhodiolae
Fermentation liquid can extend the swimming time of mice significantly, and the motion thus pointing out Radix Rhodiolae fermentation liquid can significantly improve mice is resistance to
Power.
Radix Rhodiolae fermentation liquid mice is swum before and after Serum lactic acid content and production of lactic acid and the impact of degradation rate
As shown in Table 2, after each group mouse stomach 10d, before mouse movement, between each group of blood lactase acid value, there is no notable difference.Mice
After carrying out the strenuous exercise of short time (20min), C group and A group do not have difference, B group blood lactase acid value to be all remarkably higher than matched group,
No significant difference between each administration group.
By table 2 it has been also found that after motion rest 40min, compared with A group, the blood lactase acid value (blood lactase acid of B, C group mice
III) all significantly reduce.Although B, C group blood lactase acid generating rate is significantly higher than A group, but the equal C of blood lactase acid degradation rate of B group
Group significantly increases, and the ability of prompting Radix Rhodiolae fermentation liquid aerobic metabolism is strengthened.
Table 2 Radix Rhodiolae fermentation liquid is on the impact of Serum lactic acid content before and after mice swimming
(mmol/L)
The accumulation of metabolite is that body produces tired main cause, and wherein, the increase with H+ concentration of piling up of lactic acid is important
Factor.Violent physical exertion makes lactic acid pile up in vivo, and blood pH reduces, it is suppressed that phosphofructose swashs the multiple kinds of energy generations such as acid
The activity of enzyme during thanking, makes glycolysis be affected, and ATP supplies minimizing, causes muscle fatigue.After mice swimming 20min,
C group and more blank group of B treated animal Serum lactic acid content substantially increase, and production of lactic acid speed increases the most accordingly, in conjunction with B treated animal
The interpretation of result that swimming time is obviously prolonged, with Radix Rhodiolae and enzymatic hydrolysis and fermentation liquid thereof, this promotes that body anaerobic metabolism level is relevant.
After additionally mice swimming stops, 20～50min is convalescent period of blood lactase acid after motion.Convalescent period after exercise, body
Needing a large amount of oxygen of inspiration, now body is in addition to mainly with aerobic metabolism mode energy supply, the oxygen debt in strenuous exercise to be repaid,
The removing of lactic acid completes by aerobic metabolism now just.
Owing to Radix Rhodiolae fermentation liquid is in addition to containing sugar, electrolyte, include strengthening possibly together with little molecule small peptide, polymerization sugar, aminoacid
Taurine and abundant plant flavone, vitamin B group, VC and various fatsoluble vitamin, choline, the merit such as zinc of high concentration
Can there is great relation by composition.Little molecule small peptide directly can be absorbed by intestinal, energy energy supply rapidly, has accelerated motion muscle fatigue
The effect recovered.
Although disclosing embodiments of the invention for the purpose of illustration, but it will be appreciated by those skilled in the art that: without departing from this
In invention and spirit and scope of the appended claims, various replacements, to change and modifications be all possible, therefore, and the present invention's
Scope is not limited to embodiment disclosure of that.
Claims (6)
1. the preparation method of a Radix Rhodiolae fermentation liquid, it is characterised in that: comprise the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:5, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, after 80 DEG C of 15min that precook, add and adjust the total of pH value 5.5, addition food and water with citric acid
The 0.02% pectase 55 DEG C insulation enzymolysis 2h of weight, adds 1% hemicellulase of the gross weight of food and water, adds food
With 0.56% cellulase of the gross weight of water, add 0.5% papain of the gross weight of food and water, add food and water
0.04% phytase 1h of gross weight, mix Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filter, sterilization, obtain food enzymolysis solution, stand-by；
(2) preparing Radix Rhodiolae extracting solution: by Radix Rhodiolae water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds water and beats
Slurry, then mixes with moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times, decocts, and extracts supernatant,
Obtain Radix Rhodiolae extracting solution, stand-by；
(3) the preparation of Radix Rhodiolae nutritional solution: food enzymolysis solution is mixed by weight the proportions for 4:1 with Radix Rhodiolae extracting solution,
Mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Radix Rhodiolae nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Radix Rhodiolae nutritional solution, Radix Rhodiolae nutritional solution and culture fluid weight ratio are 10:1, add breast
Acid bacterium, sodium chloride and acetic acid mixed fermentation 1day, add above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight lactic acid bacteria,
Sodium chloride and acetic fermentation, obtain fermentation liquid；
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
The preparation method of Radix Rhodiolae fermentation liquid the most according to claim 1, it is characterised in that: the food enzyme that step is (1) described
The content of the solid content solving liquid is 8%～10%.
The preparation method of Radix Rhodiolae fermentation liquid the most according to claim 1, it is characterised in that: step (2) described in red scape
The solid content of it extracting solution is 4%～6%.
The preparation method of Radix Rhodiolae fermentation liquid the most according to claim 3, it is characterised in that: step (2) described in red scape
My god, moringa powder, Radix Glycyrrhizae, Fructus Amomi, Rhizoma Polygonati Odorati, the parts by weight of Semen Raphani be respectively as follows: 20～25 parts, 5～10 parts, 1～2 part,
1～2 part, 1～2 part, 1～2 part.
The preparation method of Radix Rhodiolae fermentation liquid the most according to claim 1, it is characterised in that: the (3) described lactic acid bacteria of step,
Sodium chloride, the parts by weight of total consumption of acetic acid are respectively 100～120 parts, 8～10 parts, and acetic acid is 4～6 parts.
The preparation method of Radix Rhodiolae fermentation liquid the most according to claim 5, it is characterised in that: the culture fluid that step is (3) described
Preparation method be: maltose is soluble in water, stand after 20min and put into compound vitamin, the post-heating that stirs boils,
Adding Ovum Gallus domesticus album after being cooled to room temperature, stir, stand-by, maltose, water, compound vitamin, the weight ratio of Ovum Gallus domesticus album are
100:10000:3:10。
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