CN105970633A - Biological composite enzyme preparation for textiles, as well as preparation method and application of biological composite enzyme preparation - Google Patents
Biological composite enzyme preparation for textiles, as well as preparation method and application of biological composite enzyme preparation Download PDFInfo
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- CN105970633A CN105970633A CN201610450737.2A CN201610450737A CN105970633A CN 105970633 A CN105970633 A CN 105970633A CN 201610450737 A CN201610450737 A CN 201610450737A CN 105970633 A CN105970633 A CN 105970633A
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- Prior art keywords
- enzyme preparation
- preparation
- enzyme
- compound bio
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- 238000002360 preparation method Methods 0.000 title claims abstract description 105
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 64
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 64
- 239000004753 textile Substances 0.000 title abstract description 14
- 239000002131 composite material Substances 0.000 title abstract description 9
- 229940088598 enzyme Drugs 0.000 claims abstract description 129
- 150000001875 compounds Chemical class 0.000 claims abstract description 91
- 238000000034 method Methods 0.000 claims abstract description 36
- 108010059892 Cellulase Proteins 0.000 claims abstract description 25
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 25
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 25
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 25
- 229940106157 cellulase Drugs 0.000 claims abstract description 25
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 24
- 239000004094 surface-active agent Substances 0.000 claims abstract description 22
- 238000009990 desizing Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000007670 refining Methods 0.000 claims abstract description 9
- 239000003755 preservative agent Substances 0.000 claims abstract description 5
- 230000002335 preservative effect Effects 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 37
- 239000000203 mixture Substances 0.000 claims description 32
- 238000000855 fermentation Methods 0.000 claims description 27
- 230000004151 fermentation Effects 0.000 claims description 27
- 239000004744 fabric Substances 0.000 claims description 22
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical group [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 18
- 239000004299 sodium benzoate Substances 0.000 claims description 18
- 235000010234 sodium benzoate Nutrition 0.000 claims description 18
- 238000009941 weaving Methods 0.000 claims description 7
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 6
- 150000002191 fatty alcohols Chemical class 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 238000009833 condensation Methods 0.000 claims description 4
- 230000005494 condensation Effects 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 4
- -1 polysiloxanes Polymers 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 3
- 241000193755 Bacillus cereus Species 0.000 claims description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 241000235648 Pichia Species 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 238000013019 agitation Methods 0.000 claims description 2
- 235000019387 fatty acid methyl ester Nutrition 0.000 claims description 2
- 229920001296 polysiloxane Polymers 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000000600 sorbitol Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 229940111205 diastase Drugs 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 42
- 239000001110 calcium chloride Substances 0.000 abstract description 25
- 229910001628 calcium chloride Inorganic materials 0.000 abstract description 25
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract description 18
- 235000011121 sodium hydroxide Nutrition 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000003513 alkali Substances 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 108010059820 Polygalacturonase Proteins 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 108010093305 exopolygalacturonase Proteins 0.000 abstract description 2
- 238000004065 wastewater treatment Methods 0.000 abstract 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 36
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
- 230000000052 comparative effect Effects 0.000 description 27
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 15
- 229920000742 Cotton Polymers 0.000 description 10
- 238000004061 bleaching Methods 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 10
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 8
- 239000002253 acid Substances 0.000 description 8
- 239000011734 sodium Substances 0.000 description 8
- 229910052708 sodium Inorganic materials 0.000 description 8
- 238000005406 washing Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000000306 component Substances 0.000 description 5
- 239000006052 feed supplement Substances 0.000 description 5
- 239000000835 fiber Substances 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 108010029541 Laccase Proteins 0.000 description 4
- 230000001133 acceleration Effects 0.000 description 4
- 238000002203 pretreatment Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 3
- 229940059442 hemicellulase Drugs 0.000 description 3
- 108010002430 hemicellulase Proteins 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 229920006395 saturated elastomer Polymers 0.000 description 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- BQSLGJHIAGOZCD-CIUDSAMLSA-N Leu-Ala-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O BQSLGJHIAGOZCD-CIUDSAMLSA-N 0.000 description 2
- 101150051118 PTM1 gene Proteins 0.000 description 2
- 239000004115 Sodium Silicate Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 229910052925 anhydrite Inorganic materials 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 239000013587 production medium Substances 0.000 description 2
- 238000009991 scouring Methods 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 2
- 229910052911 sodium silicate Inorganic materials 0.000 description 2
- HQAITFAUVZBHNB-UHFFFAOYSA-N sodium;pentahydrate Chemical compound O.O.O.O.O.[Na] HQAITFAUVZBHNB-UHFFFAOYSA-N 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 229910004835 Na2B4O7 Inorganic materials 0.000 description 1
- 239000007832 Na2SO4 Substances 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000009874 alkali refining Methods 0.000 description 1
- 150000001447 alkali salts Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003518 caustics Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 229940097267 cobaltous chloride Drugs 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
- D06L1/00—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods
- D06L1/12—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods using aqueous solvents
- D06L1/14—De-sizing
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
- D06M11/07—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with halogens; with halogen acids or salts thereof; with oxides or oxyacids of halogens or salts thereof
- D06M11/11—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with halogens; with halogen acids or salts thereof; with oxides or oxyacids of halogens or salts thereof with halogen acids or salts thereof
- D06M11/13—Ammonium halides or halides of elements of Groups 1 or 11 of the Periodic Table
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
- D06M13/184—Carboxylic acids; Anhydrides, halides or salts thereof
- D06M13/188—Monocarboxylic acids; Anhydrides, halides or salts thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/04—Vegetal fibres
- D06M2101/06—Vegetal fibres cellulosic
Landscapes
- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention provides a biological composite enzyme preparation for textiles, as well as a preparation method and application of the biological composite enzyme preparation. The biological composite enzyme preparation is prepared from the following components: 1200 to 2000 U/mL of alpha-amylase, 800 to 1000 U/mL of cellulase, 8000 to 12000 U/mL of xylanase, 400 to 1200 U/mL of pectinase, 2 to 8 g/L of calcium chloride, 5 to 15 g/L of a preservative, and 50 to 100 g/L of a surfactant. The biological composite enzyme preparation, the preparation method and the application have the advantages that as the biological compound enzyme preparation replaces caustic soda used in the conventional textile pretreatment process, energy consumption, water consumption, raw material alkali consumption, wastewater treatment cost and the like are greatly reduced, the cost is low, and the pH value application range and the temperature range are wide; when the biological composite enzyme preparation is used for treatment before desizing and refining of a textile, the desizing rate of a textile product can be equal to or higher than 98%, the capillary effect is high, and the whiteness is higher than 80.
Description
Technical field
The invention belongs to textile technology field, relate to a kind of compound bio-enzyme preparation and preparation method thereof and answer
With, especially relate to a kind of weaving compound bio-enzyme preparation and its preparation method and application.
Background technology
In traditional yarn fabric pre-treating technology, commonly used Caustic soda high temperature carries out desizing and refine, utilizes
Textile sizing material is expanded by Caustic soda, then is rinsed with a large amount of hot water thus reaches desizing effect；Separately
Outward, Caustic soda is utilized to decompose the pectic substance on cotton shell and protein etc., the most also can be to fatty acid ester in waxiness
Class material carries out saponification, and the middle-and-high-ranking fatty alcohol of removal of surfactant cotton fiber waxiness, higher fatty acids and
The hydrophobic contaminants such as carbohydrate, thus reach preferable refining effect.But, amount of caustic soda controls not
With great difficulty cause fibre damage, promote that the cloth intensity after refine is the highest and feel is poor, it is often more important that alkaline scouring
Method power consumption high, water consumption big, produces the waste water that a large amount of alkalescence is strong, colourity is deep, COD is high, and
The salt neutralizing alkali generation can produce impact to fabric color, and therefore, the pre-treating method of application caustic scouring is
Through the development trend of domestic and international Green Textile Base cannot be adapted to.
Application biological enzyme formulation carries out the pre-treatment of yarn fabric, can significantly improve pretreatment of spinning technique to product
Cost and the impact of environment, save the energy, using water wisely, saving production time, and waste water handling
With, meanwhile, product quality also can be improved.But, different enzyme character and specificity are different,
How to carry out the most compounding key being to ensure that application effect.
CN101624583A discloses the manufacture work of a kind of energy-saving and environment-friendly cooking and bleaching compound enzyme preparation for weaving
Skill, this compound enzyme mainly includes alpha amylase, pectase, xylanase, laccase and cellulase, utilizes
This invention the enzyme preparation of degradation of hemicellulose and lignin can will can not be used for the front place of natural fiber and fabric
Reason, can the effective fiber accompanying impurities such as depolymerized pectin, hemicellulose, lignin, and be readily cleaned, can subtract
Few washing times, reduces sewage discharge and COD value, and can effectively assist bleaching process, reduces hydrogen peroxide
Consumption, but this invention is applied laccase, cost is high, and needs when its compound enzyme uses to regulate pH
To 5.0 ± 0.2, temperature limit is narrow, and only 50 DEG C, application conditions is harsher.
Therefore, in the art, it is desirable to access and a kind of can be used in having of yarn fabric pre-treating technology
The low-cost bio compound enzymic preparation that more preferable treatment effect, application conditions are more wide in range.
Summary of the invention
For the deficiencies in the prior art, it is an object of the invention to provide a kind of a kind of compound bio-enzyme preparation and
Its preparation method and application, the weaving of particularly offer one with compound bio-enzyme preparation and preparation method thereof and is answered
With.
For reaching this purpose, the present invention by the following technical solutions:
On the one hand, the present invention provides a kind of weaving compound bio-enzyme preparation, described compound bio-enzyme preparation bag
Include the composition of following content:
In the compound bio-enzyme preparation of the present invention, alpha amylase can effectively remove the starch size on grey cloth, reaches
To preferable desizing effect；The refine of yarn fabric can be played by pectase, cellulase and hemicellulase
Well effect, pectase makes the pectic substance outside cotton fiber decompose, cellulase journey different with hemicellulase
Other hydrophobic contaminants removing cotton fiber surface of degree, thus improve the wettability of fabric, tradition can be reached
The level of alkali refining, or obtain the product of higher quality.By multiple enzyme composite usage, it is suitable for fabric fibre table
The multifarious feature of face impurity, is carried out desizing and refine simultaneously, utilizes the specificity of various enzyme and works in coordination with
Property, good desizing refining effect can be obtained, meanwhile, minimizing processes time and treatment process, processes bar
Part is gentle, little to fibre damage.
The compound bio-enzyme preparation of the present invention do not uses this relatively costly enzyme of laccase, and the present invention
PH value is adjusted without using acetic acid, the pH value range of application and temperature model during compound bio-enzyme formulation application
Enclose the most wide in range.
In the compound bio-enzyme preparation of the present invention, described α-amylase is produced by the fermentation of bacillus cereus liquid.
In the compound bio-enzyme preparation of the present invention, the content of described α-amylase is 1200～2000
U/mL, such as 1200U/mL, 1300U/mL, 1400U/mL, 1500U/mL, 1600U/mL,
1700U/mL, 1800U/mL, 1900U/mL or 2000U/mL.
In the compound bio-enzyme preparation of the present invention, the content of described cellulase is 800～1000
U/mL, such as 800U/mL, 820U/mL, 840U/mL, 860U/mL, 880U/mL, 900
U/mL, 920U/mL, 950U/mL, 980U/mL or 1000U/mL.
In the compound bio-enzyme preparation of the present invention, the content of described xylanase is 8000～12000
U/mL, such as 8000U/mL, 8200U/mL, 8400U/mL, 8600U/mL, 8800U/mL,
9000U/mL、9300U/mL、9500U/mL、9800U/mL、10000U/mL、11000U/mL
Or 12000U/mL.
Preferably, described cellulase and xylanase are produced by Pichia yeast liquid fermentation.
In the compound bio-enzyme preparation of the present invention, the content of described pectase is 400～1200U/mL,
Such as 400U/mL, 500U/mL, 600U/mL, 700U/mL, 800U/mL, 900U/mL,
1000U/mL, 1100U/mL or 1200U/mL.
Preferably, described pectase is produced by escherichia coli liquid fermentation.
In the compound bio-enzyme preparation of the present invention, the content of described calcium chloride is 2～8g/L, such as 2
g/L、2.3g/L、2.5g/L、2.8g/L、3g/L、3.5g/L、3.8g/L、4g/L、4.5g/L、4.8
G/L, 5g/L, 5.5g/L, 6g/L, 6.5g/L, 7g/L, 7.5g/L or 8g/L.
In the compound bio-enzyme preparation of the present invention, calcium chloride as pectase, the activator of xylanase and
Stabilizer, in compound enzymic preparation calcium chloride very few, lack or excess all may result in two kinds of enzymes enzyme live reduce,
Even lose enzyme to live, thus affect application effect.
In the compound bio-enzyme preparation of the present invention, the content of described preservative is 5～15g/L, such as 5
G/L, 6g/L, 7g/L, 8g/L, 9g/L, 10g/L, 11g/L, 12g/L, 13g/L, 14g/L or
15g/L。
Preferably, described preservative is sodium benzoate, naturally it is also possible to apply other suitable preservative.
In the compound bio-enzyme preparation of the present invention, the content of described surfactant is 50～100g/L,
Such as 50g/L, 55g/L, 60g/L, 65g/L, 70g/L, 75g/L, 80g/L, 85g/L, 90
G/L, 95g/L or 100g/L.
Preferably, described surfactant is polysiloxanes, natural fatty alcohol and ethylene oxide adduct
The condensation substance JFC of AEO, oxirane and high fatty alcohol, fatty acid methyl ester and the condensation substance of oxirane
Any one or the combination of at least two in FMEE or sorbitol.
In compound bio-enzyme preparation of the present invention, solvent for use is water.
Compound bio-enzyme preparation prepared by the present invention pH value be 6.0-8.0 (6.0,6.2,6.4,6.6,6.8,
7.0,7.2,7.4,7.6,7.8 or 8.0), time, wherein said enzyme is provided with activity.Described compound bio-enzyme
Preparation can 20-60 DEG C (such as 20 DEG C, 22 DEG C, 25 DEG C, 28 DEG C, 30 DEG C, 33 DEG C, 36 DEG C, 40 DEG C,
45 DEG C, 48 DEG C, 50 DEG C, 55 DEG C, 58 DEG C or 60 DEG C) under apply, its application pH value and temperature range ratio
Broad.
The present invention is by for the selection of enzyme in compound bio-enzyme and compounding, utilizing α-amylase, cellulose
Enzyme, xylanase and pectase carry out cooperation with special ratios and make between different enzyme component mutually coordinated, no
But the effect of various enzyme can be given full play to, and synergism each other, strengthen the effect effect of compound enzyme
Really, and with the use of calcium chloride, sodium benzoate and surfactant compound enzyme is further enhanced to textile
Effect of pre-treatment, plays good effects for compound bio-enzyme and has facilitation.In compound bio-enzyme system
Under the cooperating of each composition of agent so that be applied to during yarn fabric pre-treating technology have preferably process effect
Really, improve compound enzyme application efficiency.Application conditions is more wide in range, and low cost.
On the other hand, the invention provides the preparation method of compound bio-enzyme preparation of the present invention, described system
Preparation Method is: by α-amylase, cellulase, xylanase, pectase, calcium chloride, sodium benzoate and
Surfactant mixes according to formula components content as described above, and solvent for use is water, by solid
All dissolving, liquid agitation is uniform, i.e. can get described compound bio-enzyme preparation.
The preparation method of compound bio-enzyme preparation of the present invention is simple, low cost, it is adaptable to industry metaplasia
Produce.
On the other hand, the present invention provides compound bio-enzyme preparation of the present invention to locate before yarn fabric desizing refining
Application in reason.
Preferably, described compound bio-enzyme formulation application is in cold dome or long vehicle technique.
Preferably, described compound bio-enzyme preparation is when application in yarn fabric desizing refining pre-treatment, biological
Compound enzymic preparation consumption is 10-25g/L, such as 10g/L, 12g/L, 14g/L, 16g/L, 18g/L,
20g/L, 22g/L, 24g/L or 25g/L.
Preferably, the application pH of described compound bio-enzyme preparation is 6.0-8.0, such as 6.0,6.2,6.4,
6.6,6.8,7.0,7.2,7.4,7.6,7.8 or 8.0.
Preferably, in the application, yarn fabric is being padded in compound bio-enzyme preparation.
Preferably, described in the temperature padded be 20-60 DEG C, such as 20 DEG C, 22 DEG C, 25 DEG C, 28 DEG C,
30 DEG C, 33 DEG C, 36 DEG C, 40 DEG C, 45 DEG C, 48 DEG C, 50 DEG C, 55 DEG C, 58 DEG C or 60 DEG C.
Preferably, described in the liquid carrying rate that pads be 80-100%, such as 80%, 82%, 84%, 86%,
88%, 90%, 92%, 94%, 96%, 98% or 100%.
Relative to prior art, the method have the advantages that
(1) Caustic soda during present invention application compound enzymic preparation substitutes weaving tradition pre-treating technology, saves a large amount of
Energy consumption, water consumption, raw material alkali and waste water processing cost etc..Can effectively reduce production cost, minimizing is knitted
Fibres damages, and increases fabric whiteness and capillary effect, not only has obvious social benefit, it may have important
Economic benefit.
(2) present invention does not use this relatively costly enzyme of laccase, and the compound bio-enzyme preparation of the present invention
Adjusting pH value without using acetic acid during application, pH value range and the temperature range of application are the most wide in range.
(3) by the enzyme used and the selection of content thereof so that mutually assist between different enzyme components
Adjust, not only can give full play to the effect of various enzyme, and synergism each other, strengthen compound enzyme
Action effect, and further enhance compound enzyme pair with the use of calcium chloride, sodium benzoate and surfactant
Textile carries out effect of desizing refining pre-treatment, plays good effects for compound bio-enzyme and has promotion work
With.After making to process, the desizing rate of fabric can reach >=98%, and capillary effect is high, and whiteness can reach ＞ 80.
Detailed description of the invention
Technical scheme is further illustrated below by detailed description of the invention.Those skilled in the art
It will be clearly understood that the only help of described embodiment understands the present invention, it is not construed as the concrete limit to the present invention
System.
Embodiment 1
In the present embodiment, using liquid submerged fermentation method to produce α-amylase, method and condition are as follows:
Enzymatic production medium component: Semen Maydis powder 2%, peptone 3%, CaCl20.07%, Na2HPO4
0.8%, pH 7.0.
2000L ferment tank condition of enzyme production: inoculum concentration 2%, fills liquid coefficient 0.65, temperature 37 DEG C, rotating speed
100-200r/min, ventilation 1:0.5-1:2.5, make dissolved oxygen maintain 20-30%.The most automatic
Feeding ammonia water and hydrochloric acid, make fermentation liquid pH value maintain 7.0.Meanwhile, the feed supplement side that intermittent flow adds is used
Formula, maintains the remaining sugar concentration in fermentation liquid.Finally, fermentation 30h reaches to produce enzyme peak, alpha-amylase activity
Up to 2000-2500U/mL.
Embodiment 2
In the present embodiment, liquid submerged fermentation method is used to produce cellulase and hemicellulase, method
And condition is as follows:
BSM basal medium:
For the culture medium of 1L, mixing adds following ingredients and makes BMGY basal medium: the phosphorus of 85%
Acid 26.7mL, CaSO4 0.93g、K2SO4 18.2g、MgSO414.9g, KOH 4.13g, glycerol 40g,
(NH4)2SO410g, water adds to 1L.
PTM1 basis salt: CuSO4·5H2O 6g, sodium iodide 0.08g, MnSO4·H2O 3.0g, molybdic acid
Sodium 2H2O 0.2g, boric acid 0.02g, cobaltous chloride 0.5g, ZnCl2 20.0g、FeSO465.0g, biotin 0.2g,
H2SO45mL, adds water to 1L.
2000L ferment tank condition of enzyme production:
Glycerol batch fermentation:
(1), by fermentation tank and the fermentation basis salts media sterilizing that comprises 4% glycerol.
(2), after sterilizing and cooling, when temperature is down to 30 DEG C, stirring and ventilation are opened to operating ring
Border (usually maximum (top) speed and 0.1-1.0vvm) and with 28% ammonia (undiluted) by the pH of culture medium
It is adjusted to 5.0.Every liter of culture medium adds the aseptic PTM1 basis salt of 4.35ml.
(3) from seed tank, the seed liquor of preliminary examination fermentation volume 5-10%, is inoculated in fermentation tank.Note
Cultivate and start first DO value close to 100%.Oxygen can be consumed after starting to cultivate, cause DO value to decline,
Add required oxygen to guarantee that DO value is more than 20%.
(4), carrying out batch fermentation until glycerol is totally consumed (18-24h), mark is that DO value increases
To 100%.Notice that the time that glycerol consumes completely can change along with preliminary examination fermentation liquid density.
Glycerol feed-batch culture:
(1) the every liter of glycerol containing 12mlPTM1, adding 50%w/v carries out feed supplement.Feed rate is set
For 18.15mL preliminary examination fermentating liquid volume per liter per hour.
(2), glycerol feed supplement will carry out about 4h or longer.
Methanol feeding is cultivated:
(1), terminate the feed supplement of glycerol and start with 100% containing the basic salt of 12mlPTM1 every liter
Methanol is induced.Set feed rate as 3.6ml/h every liter preliminary examination fermentating liquid volume.
(2), in the 2-3h started, methanol can be accumulated in fermentation liquid, and culture adapts to the process of methanol
Middle oxygen dissolving value can change.
(3) if DO value can not maintain more than 20%, stop stream and add methanol, DO value stabilization by the time
Rear continuation adds methanol with present rate stream.Increase stirring, ventilation, pressure or the supplementary of oxygen and make DO
Maintain more than 20%.
(4), when culture adapt to utilize completely methanol (2-4h) and methanol become its restricted growth because of
After son, it will have a stable DO reading and one DO point stabilization time faster (typically less than
1min).Culture adapt to will to flow after methanol rate of acceleration double before at restrictive condition under keep this relatively low
The minimum 1h of stream rate of acceleration.Then stream rate of acceleration doubles about 7.3ml/h every liter Preliminary fermentation liquid
Long-pending.
(5), after adding 2h with the speed stream of 7.3ml/h/L, increase methanol feeding speed to 10.9ml/h
Every liter of Preliminary fermentation liquid amasss.This stream rate of acceleration runs through remaining ferment process.
Finally, fermentation 96h reach produce enzyme peak, cellulase and Xylanase activity respectively up to
2500-3000U/mL and 10000-20000U/mL.
Embodiment 3
In the present embodiment, using liquid submerged fermentation method to produce pectase, method and condition are as follows:
Enzymatic production medium component (g/L): peptone 30.0, yeast powder 20.0, NaCl 5.0, Na2SO4
2.0, (NH4)2SO42.5, NH4Cl 0.5, (NH4)3C6H5O71.08, K2HPO414.6, NaH2PO4
·2H2O 3.6, MgSO4·7H2O 2.0, glycerol 10.0, trace element solution 1.0mL/L, pH 7.0.
Trace element solution composition: FeSO4·7H2O 10g/L, ZnSO4·7H2O 2.25g/L, CuSO4·
5H2O 1.0g/L, MnSO4·H2O 0.38g/L, Na2B4O7·10H2O 0.23g/L, and CaSO4·
2H2O 3.1g/L。
Supplemented medium (g/L): glycerol 500, MgSO4·7H2O 3.4, yeast powder 50, peptone
50。
2000L ferment tank condition of enzyme production: inoculum concentration 10%, fills liquid coefficient 0.45, temperature 25 DEG C, turns
Speed 100-200r/min, ventilation 1:0.5-1:2.5, make dissolved oxygen maintain 20-30%.During the fermentation certainly
Dynamic Feeding ammonia water and hydrochloric acid, make fermentation liquid pH value maintain 7.0.Meanwhile, the feed supplement side that intermittent flow adds is used
Formula, maintains the glycerol concentration in fermentation liquid.Finally, fermentation 48h reaches to produce enzyme peak, and pectinase activity can
Reach 1000-2000U/mL.
Embodiment 4
In the present embodiment, use embodiment 1-3 prepare α-amylase, cellulase, xylanase and
Pectase and coordinate calcium chloride, sodium benzoate and surfactant to prepare compound bio-enzyme preparation, described life
Thing compound enzymic preparation includes following composition:
Preparation method is: by α-amylase, cellulase, xylanase, pectase, calcium chloride, benzene first
Acid sodium and surfactant mix according to proportioning, obtain compound bio-enzyme preparation.
Embodiment 5
In the present embodiment, use embodiment 1-3 prepare α-amylase, cellulase, xylanase and
Pectase and coordinate calcium chloride, sodium benzoate and surfactant to prepare compound bio-enzyme preparation, described life
Thing compound enzymic preparation includes following composition:
Preparation method is: by α-amylase, cellulase, xylanase, pectase, calcium chloride, benzene first
Acid sodium and surfactant mix according to proportioning, obtain compound bio-enzyme preparation.
Embodiment 6
In the present embodiment, use embodiment 1-3 prepare α-amylase, cellulase, xylanase and
Pectase and coordinate calcium chloride, sodium benzoate and surfactant to prepare compound bio-enzyme preparation, described life
Thing compound enzymic preparation includes following composition:
Preparation method is: by α-amylase, cellulase, xylanase, pectase, calcium chloride, benzene first
Acid sodium and surfactant mix according to proportioning, obtain compound bio-enzyme preparation.
Embodiment 7
In the present embodiment, use embodiment 1-3 prepare α-amylase, cellulase, xylanase and
Pectase and coordinate calcium chloride, sodium benzoate and surfactant to prepare compound bio-enzyme preparation, described life
Thing compound enzymic preparation includes following composition:
Preparation method is: by α-amylase, cellulase, xylanase, pectase, calcium chloride, benzene first
Acid sodium and surfactant mix according to proportioning, obtain compound bio-enzyme preparation.
Embodiment 8
In the present embodiment, use embodiment 1-3 prepare α-amylase, cellulase, xylanase and
Pectase and coordinate calcium chloride, sodium benzoate and surfactant to prepare compound bio-enzyme preparation, described life
Thing compound enzymic preparation includes following composition:
Preparation method is: by α-amylase, cellulase, xylanase, pectase, calcium chloride, benzene first
Acid sodium and surfactant mix according to proportioning, obtain compound bio-enzyme preparation.
Comparative example 1
Difference from Example 4 is, does not use α-amylase, containing of remaining composition in this comparative example
Measure the same as in Example 4.
Comparative example 2
Difference from Example 4 is, does not use α-amylase, improve another in this comparative example
The unit of activity content of enzyme, the content of such as cellulase is that the content of 2100U/mL or xylanase is
The content of 11200U/mL or pectase is 2000U/mL, the content of remaining composition and embodiment 4 phase
With.
Comparative example 3
Difference from Example 4 is, does not use cellulase, containing of remaining composition in this comparative example
Measure the same as in Example 4.
Comparative example 4
Difference from Example 4 is, does not use cellulase, improve another in this comparative example
The unit of activity content of enzyme, the content of such as α-amylase is that the content of 2100U/mL or xylanase is
The content of 10900U/mL or pectase is 1700U/mL, the content of remaining composition and embodiment 4 phase
With.
Comparative example 5
Difference from Example 4 is, does not use xylanase, containing of remaining composition in this comparative example
Measure the same as in Example 4.
Comparative example 6
Difference from Example 4 is, does not use pectase, the content of remaining composition in this comparative example
The same as in Example 4.
Comparative example 7
Difference from Example 4 is, does not use pectase, improve another enzyme in this comparative example
Unit of activity content, the content of such as α-amylase be the content of 2000U/mL or cellulase be 1700
The content of U/mL or xylanase is 10800U/mL, and the content of remaining composition is the same as in Example 4.
Comparative example 8
Difference from Example 4 is, does not use calcium chloride and sodium benzoate and table in this comparative example
Face activating agent, the content of remaining composition is the same as in Example 4.
Comparative example 9
Difference from Example 4 is, does not use calcium chloride and sodium benzoate in this comparative example, remaining
The content of composition is the same as in Example 4.
Comparative example 10
Difference from Example 4 is, does not use calcium chloride, the content of remaining composition in this comparative example
The same as in Example 4.
Comparative example 11
Difference from Example 4 is, does not use sodium benzoate, containing of remaining composition in this comparative example
Measure the same as in Example 4.
Embodiment 9
In the present invention, the compound bio-enzyme preparation of embodiment 1-5 and comparative example 1-11 is used for textile
Cold pad-batch pretreatment, its method is following (for embodiment 1-5 and the compound bio-enzyme of comparative example 1-11
Preparation is when pre-treating technology, and the process conditions keeping each process step are identical, to facilitate comparison different
The treatment effect of compound enzymic preparation):
Cotton grey cloth (specification 20 × 16) enzyme process desizing refining, bleaching
Technological process: enzyme liquid configures → pad enzyme liquid → cold dome → washing → bleaching → washing → drying
Compound bio-enzyme preparation consumption: 10-25g/L, during application, system pH is 6.0-7.0.
Padding enzyme liquid: pad in enzyme liquid at 20 DEG C-60 DEG C, an immersing and rolling, liquid carrying rate is
80-100%.
Cold dome: cold dome 6-12h at a temperature of 20 DEG C-40 DEG C.
Washing: cleaning 2-4 road with the hot water that temperature is 70 DEG C-80 DEG C, cold water washes 1 road.
Bleaching: (composition is metasilicate pentahydrate sodium, inclined silicon to the supporting special oxygenic bleaching aids 10-25g/L of enzyme
2-3 kind in acid sodium, sodium silicate, SDS, LAS), hydrogen peroxide 5-20g/L, chelating agen 2-10g/L,
Penetrating agent 2-10g/L (one or more in JFC, tween-61, FMEE, FMS, SDS and LAS)；
Liquid carrying rate is 80-100%, steams 10-30min under 100 DEG C of saturated vapors.
After process, the desizing rate of cotton, capillary effect and whiteness test result are as shown in table 1.
Table 1
From table 1 it follows that by the compound bio-enzyme preparation (embodiment 4-8) of the present invention for textile
Cold pad-batch pretreatment, after process, the desizing rate of cotton can reach >=98%；Capillary effect: broadwise
11-12cm/30min (5cm width), warp-wise 10-11cm/10min (5cm width)；Whiteness: ＞ 80.And
When lacking a kind of enzyme in compound bio-enzyme preparation, other enzyme content are constant, or the another kind of enzyme of raising contains
During amount (comparative example 1-7), desizing rate, capillary effect and whiteness all can reduce, and these several enzymes in the present invention are described
Synergism enhances the treatment effect for yarn fabric.And when compound enzymic preparation does not use calcium chloride and
When sodium benzoate and surfactant, or when not using one therein, can not reach preferably to locate
Reason effect, therefore explanation calcium chloride and sodium benzoate and surfactant are for improving the place of compound enzymic preparation
Reason effect has also played facilitation.
Embodiment 10
In the present invention, compound bio-enzyme preparation being used for textile long vehicle pre-treating technology, its method is as follows
(for embodiment 1-5 and comparative example 1-11 compound bio-enzyme preparation for pre-treating technology time, keep
The process conditions of each process step are identical, to facilitate the treatment effect comparing different composite enzyme preparation):
Cotton grey cloth (specification 20 × 16) enzyme process desizing refining, bleaching
Technological process: enzyme liquid configures → pad enzyme liquid → decatize → washing → bleaching → washing → drying
Compound bio-enzyme preparation consumption: 10-25g/L, during application, system pH is 6.0-7.0.
Padding enzyme liquid: pad in enzyme liquid at 20 DEG C-60 DEG C, two leachings two are rolled, and liquid carrying rate is
80-100%.
Decatize: steam 0.5-1h in the saturated vapor of 55 DEG C of-65 DEG C of temperature.
Washing: cleaning 2-4 road with the hot water that temperature is 70 DEG C-80 DEG C, cold water washes 1 road.
Bleaching: (composition is metasilicate pentahydrate sodium, inclined silicon to the supporting special oxygenic bleaching aids 10-25g/L of enzyme
2-3 kind in acid sodium, sodium silicate, SDS, LAS), hydrogen peroxide 5-20g/L, chelating agen 2-10g/L,
Penetrating agent 2-10g/L (one or more in JFC, tween-61, FMEE, FMS, SDS and LAS)；
Liquid carrying rate is 80-100%, steams 10-30min under 100 DEG C of saturated vapors.
After process, the desizing rate of cotton, capillary effect and whiteness test result are as shown in table 2.
Table 2
From Table 2, it can be seen that by the compound bio-enzyme preparation (embodiment 4-8) of the present invention for textile
Long vehicle pre-treating technology, after process, the desizing rate of cotton can reach >=98%；Capillary effect: broadwise 10cm/30min
(5cm width), warp-wise 10cm/15min (5cm width)；Whiteness ＞ 80, and when in compound bio-enzyme preparation
Lacking a kind of enzyme, other enzyme content are constant, or when improving another kind of enzyme content (comparative example 1-7),
Desizing rate, capillary effect and whiteness all can reduce, illustrate in the present invention these several enzyme synergism enhance for
The treatment effect of yarn fabric.And live when compound enzymic preparation does not use calcium chloride and sodium benzoate and surface
During property agent, or when not using one therein, preferable treatment effect can not be reached, calcium chloride is described
With sodium benzoate and surfactant, promotion is also played for the treatment effect improving compound enzymic preparation to make
With.
Applicant state, the present invention by above-described embodiment illustrate the present invention compound bio-enzyme preparation and
Preparation method and application, but the invention is not limited in above-described embodiment, i.e. do not mean that the present invention must depend on
Above-described embodiment is relied to implement.Person of ordinary skill in the field is it will be clearly understood that any to the present invention
Improving, the equivalence of raw material each to product of the present invention is replaced and the interpolation of auxiliary element, concrete way choice
Deng, within the scope of all falling within protection scope of the present invention and disclosure.
Claims (10)
1. compound bio-enzyme preparation is used in a weaving, it is characterised in that described compound bio-enzyme preparation includes
The composition of following content:
Compound bio-enzyme preparation the most according to claim 1, it is characterised in that described α-amylase
For mesophilicα-diastase.
Compound bio-enzyme preparation the most according to claim 1 and 2, it is characterised in that described alphalise starch
Enzyme is produced by the fermentation of bacillus cereus liquid；
Preferably, described cellulase and xylanase are produced by Pichia yeast liquid fermentation；
Preferably, described pectase is produced by escherichia coli liquid fermentation.
4. according to the compound bio-enzyme preparation according to any one of claim 1-3, it is characterised in that described
Preservative is sodium benzoate；
Preferably, described surfactant is polysiloxanes, natural fatty alcohol and ethylene oxide adduct
The condensation substance JFC of AEO, oxirane and high fatty alcohol, fatty acid methyl ester and the condensation substance of oxirane
Any one or the combination of at least two in FMEE or sorbitol.
5. according to the preparation method of the compound bio-enzyme preparation according to any one of claim 1-4, its feature
Being, described preparation method is: by α-amylase, cellulase, xylanase, pectase, chlorination
Calcium, sodium benzoate and surfactant mix according to component content as claimed in claim 1, used
Solvent is water, is all dissolved by solid, and liquid agitation is uniform, i.e. can get described compound bio-enzyme preparation.
6. according to the compound bio-enzyme preparation according to any one of claim 1-5 before yarn fabric desizing refining
Application in process.
Application the most according to claim 6, it is characterised in that described compound bio-enzyme formulation application
In cold dome or long vehicle technique.
Application the most according to claim 6, it is characterised in that described compound bio-enzyme preparation consumption
For 10-25g/L；
Preferably, the application pH of described compound bio-enzyme preparation is 6.0-8.0.
Application the most according to claim 6, it is characterised in that by yarn fabric in compound bio-enzyme
Preparation pads.
Application the most according to claim 9, it is characterised in that described in the temperature padded be
20-60℃；
Preferably, the liquid carrying rate padded described in is 80-100%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107740280A (en) * | 2017-09-15 | 2018-02-27 | 江苏新瑞贝科技股份有限公司 | A kind of enzyme pretreating reagent and processing method for cotton fiber |
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| CN111809397A (en) * | 2020-06-30 | 2020-10-23 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for low-temperature treatment of ready-made clothes and after-finishing method of cotton-type fabric ready-made clothes |
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| CN107740280A (en) * | 2017-09-15 | 2018-02-27 | 江苏新瑞贝科技股份有限公司 | A kind of enzyme pretreating reagent and processing method for cotton fiber |
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| CN112832031A (en) * | 2020-03-27 | 2021-05-25 | 中国科学院天津工业生物技术研究所 | Biological compound for improving fabric capillary effect and preparation method and application thereof |
| CN112832031B (en) * | 2020-03-27 | 2023-02-28 | 中国科学院天津工业生物技术研究所 | Biological compound for improving fabric capillary effect and preparation method and application thereof |
| CN111809397A (en) * | 2020-06-30 | 2020-10-23 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for low-temperature treatment of ready-made clothes and after-finishing method of cotton-type fabric ready-made clothes |
| CN113174678A (en) * | 2021-04-28 | 2021-07-27 | 一木生态纺织品(江苏)有限公司 | Plant fiber blended fabric and processing method thereof |
| CN113174678B (en) * | 2021-04-28 | 2021-12-07 | 一木生态纺织品(江苏)有限公司 | Plant fiber blended fabric and processing method thereof |
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