CN105969581B - Pueraria lobata cordyceps militaris liquor and preparation method thereof - Google Patents
Pueraria lobata cordyceps militaris liquor and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of pueraria lobata cordyceps militaris liquors, mainly by puerarin saccharification liquid and Cordceps militaris fermentation liquid by 1:(0.25-3) quality proportioning form fermentation raw material, make and obtain pueraria lobata cordyceps militaris liquor.Pueraria lobata cordyceps militaris liquor of the present invention has mellowness, strong smell, pure and mild, tasty and refreshing mouthfeel, the wine body quality of coordination and significant health-care efficacy.In addition, pueraria lobata cordyceps sinensis method for preparing medicated wine of the invention, provides advantageous condition to obtain all good Cordceps militaris health liquor of mouthfeel, quality, and operation is convenient, raw material sources are extensive, low in cost, are suitable for large-scale production and application.
Description
Technical field
The present invention relates to health liquor technical field, in particular to a kind of pueraria lobata cordyceps militaris liquor, meanwhile, the invention further relates to the Pueraria lobotas
The preparation method of root cordyceps militaris liquor.
Background technique
Cordceps militaris, also known as northern Chinese caterpillar Fungus, Cordyceps militaris, ingredient, pharmacology and clinical effectiveness are identical as cordyceps sinensis, have
It is the effects of guarantor's lung kidney-nourishing, hemostasis and phlegm, adjusting physical function, edible throughout the year because its pharmacological property is mild, than other types
Invigorant have wider medical value.
Pueraria lobata is the root of legume pueraria lobata, containing about 12% flavone compound, including it is soybean (soya bean) glucoside, big
Beans glucoside member, Puerarin etc. more than 10；And containing substances such as Daucosterol, amino acid, Coumarins, medicinal application can be used as,
Main component is starch, the minerals such as protein rich in, amino acid, sugar and iron needed by human, calcium, copper, selenium, also
It is rare invigorant suitable for people of all ages, there is the good reputation of " thousand Nian Rencan ".
Currently, the health liquor existing research developed using raw materials such as cordyceps sinensis, pueraria lobatas, such as Chinese patent
ZL200910044580.3 discloses a kind of pueraria lobata Chinese caterpillar fungus health wine and its brewing method, be by cordyceps sinensis, ganoderma lucidum, fructus lycii and
Pueraria lobata mixing is used as liquor-making raw material, and inoculation saccharomyces cerevisiae carries out fermented soy, because the starch in raw material is difficult to be utilized in saccharomyces cerevisiae,
The health liquor alcoholic strength of brewing is not high, and the functional compounds such as flavonoids are difficult to dissolve out, and cause the ineffective of health liquor.It is existing
Some cordyceps militaris liquors also generally existing high production cost, effect difference and finished product heavy bitter taste, the problems such as mouthfeel is not good enough
Summary of the invention
To solve the deficiencies in the prior art, the present invention provides a kind of pueraria lobata cordyceps militaris liquors, have good wine body
Quality and health-care efficacy.
To achieve the above object, pueraria lobata cordyceps militaris liquor of the invention mainly presses 1 by puerarin saccharification liquid and Cordceps militaris fermentation liquid:
The quality proportioning of (0.25-3) forms fermentation raw material, and brewing obtains pueraria lobata cordyceps militaris liquor.
The present invention is used to make the fermentation raw material of pueraria lobata cordyceps militaris liquor, mainly by puerarin saccharification liquid and Cordceps militaris zymotic fluid group
At this raw material of one side puerarin saccharification liquid is more conducive to the progress of brewing fermentation, not only solves main component starch in pueraria lobata
The problem of strain that is not easy to be made wine utilizes, can more assign the good fragrance of fermented wine, mouthfeel and wine body quality；On the other hand northern worm
Careless fermentation liquid makes nutrition in cordyceps sinensis, functional materials effectively be released, be proliferated, and can give full play to the medicinal nourishing function of Cordceps militaris
Effect；Another further aspect can promote flavonoids, cordycepin in pueraria lobata and Cordceps militaris in the alcohol that brewing fermentation process is constantly converted to
The dissolution of equal functional materials, and in the substances and Cordceps militaris fermentation liquid such as Puerarin, Pueraria Flavonid in puerarin saccharification liquid
The substances such as cordycepin can mutually promote dissolution, and functionally be combined with each other, preferably improve the health-care efficacy of wine.Therefore
Pueraria lobata cordyceps militaris liquor of the invention has mellowness, strong smell, pure and mild, tasty and refreshing mouthfeel, the wine body quality of coordination, Yi Jixian
The health-care efficacy of work.
As the restriction to aforesaid way, the total sugar content of the puerarin saccharification liquid is 200-400g/L；The Cordceps militaris
The thallus weight in wet base of fermentation liquid is 20-30g/L, cordycepin content is 98-160 μ g/L, cordyceps sinensis Thick many candies content is 4-6g/L.
As the restriction to aforesaid way, the fermentation condition of the pueraria lobata cordyceps militaris liquor are as follows: using saccharomyces cerevisiae as fermenting microbe,
Inoculum concentration is 0.08%-0.15% (w/w), and fermentation temperature is 25-30 DEG C, and fermentation time is main fermentation 7-10 days, post-fermentation 10-
20 days.
The performance indicator and fermentation condition for further limiting puerarin saccharification liquid and Cordceps militaris fermentation liquid, make pueraria lobata cordyceps militaris liquor
With better wine body quality, and the effect of functional materials in wine is made to be not fully exerted, improves the health care of pueraria lobata cordyceps militaris liquor
Effect.
As the restriction to aforesaid way, the alcoholic strength content of the pueraria lobata cordyceps militaris liquor is 6%-13%, flavones content
300-770mg/L, pH value 3.5-4.6.
Meanwhile the present invention also provides a kind of preparation methods of pueraria lobata cordyceps militaris liquor, method includes the following steps:
A, puerarin saccharification liquid is obtained: by kudzu-vine root powder after gelatinization, ɑ-amylase enzymolysis, carbohydrase enzymatic hydrolysis, then destroy the enzyme treatment
Obtain puerarin saccharification liquid；
B, it obtains Cordceps militaris fermentation liquid: Cordceps militaris is inoculated in potato fluid nutrient medium, shaking table culture obtains Cordceps militaris
Seed liquor, then Cordceps militaris seed liquor is inoculated in Cordceps militaris fermentation culture, shaking table culture obtains Cordceps militaris fermentation liquid；
C, puerarin saccharification liquid is uniformly mixed with Cordceps militaris fermentation liquid by quality proportioning 1:(0.25-3), is inoculated with 0.08%-
The saccharomyces cerevisiae of 0.15% (w/w), ferments at 25-30 DEG C, after main fermentation 7-10 days, post-fermentation 10-20 days, centrifugal treating,
Obtain pueraria lobata cordyceps militaris liquor.
Preparation method provided by the invention, it is convenient not only to operate, but also thus obtained pueraria lobata cordyceps militaris liquor quality is more excellent, more
Conducive to the performance of health-care efficacy.
As the restriction to aforesaid way, the step a the following steps are included:
Kudzu-vine root powder is taken, the distilled water of 2 times of kudzu-vine root powder volumes is added, mixes, in 90-95 DEG C of gelatinization 20min, is then added
ɑ-amylase of 0.15%-0.3% (w/w), after digesting 30-60min, cooling, 80 meshes excessively after colloid mill is ground, then plus
The carbohydrase for entering 0.15%-0.3% (w/w), in 55-60 DEG C of heat preservation 1-2h, finally heated to 95 DEG C enzyme deactivation 10- after mixing
15min obtains puerarin saccharification liquid；
As the restriction to aforesaid way, the step b the following steps are included:
B1, it prepares Cordceps militaris seed liquor: being beaten with the punch that diameter is 6mm and take 4 Cordceps militaris strains, be seeded in 100ml
In potato fluid nutrient medium, the shaking table culture 5 days under 25 DEG C, revolving speed 100r/min obtains Cordceps militaris seed liquor；
B2, it prepares Cordceps militaris fermentation liquid: the Cordceps militaris seed liquor of step b1 being seeded in Cordceps militaris fermentation culture, is connect
Amount is planted as 10% (v/v), shaking table culture 7 days under 25 DEG C of Yu Wendu, revolving speed 100r/min, obtains Cordceps militaris fermentation liquid.
As the restriction to aforesaid way, Cordceps militaris fermentation culture described in step b is prepared by the following steps:
Rice saccharified liquid is taken, the water of 2 times of rice saccharified liquid volumes is added, is uniformly mixed；Brewer's wort is taken, 0.5 times of wheat is added
The water of bud juice volume is uniformly mixed；Dregs of beans juice is taken, the water of 1 times of dregs of beans juice volume is added, is uniformly mixed；By three kinds of solution according to
The volume ratio of 1:1:1 after mixing, in 121 DEG C of high pressure sterilization 15-20min, obtains Cordceps militaris fermentation culture.
The culture medium raw material to be fermented using rice, malt, dregs of beans as Cordceps militaris, growth, breeding conducive to Cordceps militaris, and be
It obtains mouthfeel, quality all good Cordceps militaris health liquor and advantage is provided, and culture medium raw material is from a wealth of sources, low in cost,
Suitable for large-scale production and application.
As the restriction to aforesaid way, the rice saccharified liquid is prepared by the following steps: being taken rice meal, is added 4 times big
ɑ-the amylase of 0.15-0.3% (w/w), enzymatic hydrolysis is added after mixing after 90-95 DEG C of gelatinization 30-60min in the water of rice flour volume
30-60min, cooling, then cross ground 80 mesh of colloid mill, add the carbohydrase of 0.15-0.3% (w/w), after mixing in
55-60 DEG C of heat preservation 1-2h, finally heated to 95 DEG C enzyme deactivation 10-15min, and 15- is centrifuged under revolving speed 3000-4000r/min
20min, obtained supernatant are rice saccharified liquid；
The brewer's wort is prepared by the following steps: being taken malt flour, the water of 4 times of malt flour volumes is added, in 35-37 after mixing
DEG C heat preservation 30-60min, then at 50-55 DEG C of heat preservation 60-90min, finally after 60-65 DEG C of heat preservation 2.5h-3h, in revolving speed 3000-
It is centrifuged 10-15min under 4000r/min, obtained supernatant is brewer's wort；
The dregs of beans juice is prepared by the following steps: being taken bean cake powder, the water of 10 times of bean cake powder volumes is added, in 100 after mixing
DEG C heat preservation 10-15min, be cooled to 55-60 DEG C, add the papain of 0.15%-0.3% (w/w), mix, in 55-60
DEG C heat preservation 1.5h-2h after, be centrifuged 10-15min at revolving speed 3000-4000r/min, obtained supernatant be dregs of beans juice.
As the restriction to aforesaid way, after Cordceps militaris fermentation liquid described in step c is heat-treated again with puerarin saccharification
Liquid mixing, the heat treatment mode are 121 DEG C of high temperature high pressure process 20-30min.
Centrifuge RPMs most preferably 3000-4000r/min in step c, centrifugation time 10-15min.
In conclusion using technical solution of the present invention, the pueraria lobata cordyceps militaris liquor of acquisition has mellowness, strong smell, alcohol
With tasty and refreshing mouthfeel, the wine body quality of coordination and significant health-care efficacy.In addition, pueraria lobata cordyceps militaris liquor preparation side of the invention
Method provides advantageous condition to obtain all good Cordceps militaris health liquor of mouthfeel, quality, and operate it is convenient, raw material sources extensively,
It is low in cost, it is suitable for large-scale production and application.
Specific embodiment
Embodiment one
The present embodiment is related to pueraria lobata cordyceps militaris liquor and preparation method thereof.
The preparation method of pueraria lobata cordyceps militaris liquor the following steps are included:
Raw material: using rice, malt, dregs of beans and pueraria lobata as primary raw material；
Pretreatment of raw material: rice, malt, dregs of beans and pueraria lobata are dried at 60 DEG C to moisture content is 13% or so, so
It crushes respectively afterwards, crosses 60 meshes, respectively obtain rice meal, malt flour, bean cake powder and kudzu-vine root powder；
A, puerarin saccharification liquid is obtained: by kudzu-vine root powder after gelatinization, ɑ-amylase enzymolysis, carbohydrase enzymatic hydrolysis, then destroy the enzyme treatment
Obtain puerarin saccharification liquid；Concrete operations are as follows:
Kudzu-vine root powder is taken, the distilled water of 2 times of kudzu-vine root powder volumes is added, mixes, in 90-95 DEG C of gelatinization 20min, is then added
ɑ-amylase of 0.2% (w/w), after digesting 30min, cooling crosses 80 meshes after colloid mill is ground, adds 0.2% (w/
W) carbohydrase obtains puerarin saccharification liquid in 60 DEG C of heat preservations 2h, finally heated to 95 DEG C enzyme deactivation 10min after mixing；
B, it obtains cordyceps sinensis fermentation liquor: Cordceps militaris is inoculated in potato fluid nutrient medium, shaking table culture obtains Cordceps militaris kind
Sub- liquid, then Cordceps militaris seed liquor is inoculated in Cordceps militaris fermentation culture, shaking table culture obtains Cordceps militaris fermentation liquid；Specific behaviour
Make as follows:
B1, it prepares Cordceps militaris seed liquor: being beaten with the punch that diameter is 6mm and take 4 Cordceps militaris strains, be seeded in 100ml
In potato fluid nutrient medium, the shaking table culture 5 days under 25 DEG C, revolving speed 100r/min obtains Cordceps militaris seed liquor；The potato liquid
Body culture medium the preparation method comprises the following steps: taking potato decortication after claim 200g, be cut into fritter, set in Enamel jar plus distilled water
1000ml, heating boil 20-30min, with 8 layers of filtered through gauze and supply volume to 1000ml；
B2, it prepares Cordceps militaris fermentation liquid: the Cordceps militaris seed liquor of step b1 being seeded in Cordceps militaris fermentation culture, is connect
Amount is planted as 10% (v/v), shaking table culture 7 days under 25 DEG C of Yu Wendu, revolving speed 100r/min, obtains Cordceps militaris fermentation liquid；The north
Preparing for Chinese caterpillar fungus fermentation culture solution is as follows:
It prepares rice saccharified liquid: taking rice meal, the distilled water of 4 times of rice meal volumes is added, be gelatinized after mixing in 90-95 DEG C
After 20min, ɑ-amylase of 0.2% (w/w) is added, digests 30min, cools down, then mistake ground 80 mesh of colloid mill, then plus
The carbohydrase for entering 0.2% (w/w), in 60 DEG C of heat preservations 2h, finally heated to 95 DEG C enzyme deactivation 10min after mixing, and in revolving speed
It is centrifuged 15min under 4000r/min, obtained supernatant is rice saccharified liquid；
It prepares brewer's wort: taking malt flour, the distilled water of 4 times of malt flour volumes is added, kept the temperature after mixing in 35-37 DEG C
30min finally after 65 DEG C of heat preservation 3h, is centrifuged 10min at revolving speed 4000r/min, obtains then at 50-55 DEG C of heat preservation 60min
Supernatant be brewer's wort；
It prepares dregs of beans juice: taking bean cake powder, the distilled water of 10 times of bean cake powder volumes is added, in 100 DEG C of heat preservation 10- after mixing
15min is cooled to 60 DEG C, adds the papain of 0.2% (w/w), mixes, after 60 DEG C of heat preservation 2h, in revolving speed
It is centrifuged 10min under 4000r/min, obtained supernatant is dregs of beans juice；
It prepares Cordceps militaris fermentation culture: taking rice saccharified liquid, the water of 2 times of rice saccharified liquid volumes is added, be uniformly mixed；
Brewer's wort is taken, the water of 0.5 times of wort volume is added, is uniformly mixed；Dregs of beans juice is taken, the water of 1 times of dregs of beans juice volume is added, is mixed
Uniformly；By three kinds of solution according to 1:1:1 volume ratio after mixing, in 121 DEG C of high pressure sterilization 15-20min, obtain Cordceps militaris
Fermentation culture；
C, puerarin saccharification liquid is uniformly mixed with Cordceps militaris fermentation liquid by quality proportioning 1:(0.25-3), inoculation 0.08%
(w/w) saccharomyces cerevisiae ferments at 25-30 DEG C, after main fermentation 10 days, post-fermentation 10 days, is centrifuged at revolving speed 3000r/min
10min obtains pueraria lobata cordyceps militaris liquor, then carries out subsequent clarification, filling, sterilizing, cooling treatment, obtains pueraria lobata cordyceps sinensis wine product.
Embodiment two
The present embodiment is related in pueraria lobata cordyceps militaris liquor preparation process, saccharomyces cerevisiae dosage, Cordceps militaris fermentation liquid dosage and northern worm
Influence of careless these three conditional parameters of fermentation liquor treatment method to pueraria lobata cordyceps militaris liquor quality.
Using the preparation method being basically the same as the first embodiment, to saccharomyces cerevisiae dosage, Cordceps militaris fermentation liquid dosage and north
Three conditions of Chinese caterpillar fungus fermentation liquid processing method carry out orthogonal test, and test is as shown in the table:
Percentage composition is mass percentage in upper table, and the mode that heats in Cordceps militaris fermentation liquor treatment method is will be northern
For cordyceps sinensis fermentation liquor in 121 DEG C of high temperature high pressure process 20-30min, crossing milling treatment of colloid mode is by Cordceps militaris fermentation liquid by glue
Body mill grinding；It will be mixed again with puerarin saccharification liquid after Cordceps militaris fermentation liquor treatment, fermentation, brewing pueraria lobata cordyceps militaris liquor.
The pueraria lobata cordyceps militaris liquor that embodiment 2.1~2.9 is obtained, by yellow rice wine standard test indices, and carries out sense organ and comments
Valence, subjective appreciation table are as shown in the table:
By above-mentioned assessment method, the evaluation result of the pueraria lobata cordyceps militaris liquor of embodiment 2.1~2.9 as shown in the table is obtained:
As seen from the above table, it mixes, inoculates according to 1:1 ratio with puerarin saccharification liquid after Cordceps militaris fermentation liquid is heat-treated
The saccharomyces cerevisiae of 0.08% (w/w), ferments, and the pueraria lobata cordyceps militaris liquor quality of acquisition is best.The pueraria lobata cordyceps militaris liquor of acquisition has
Mellowness, strong smell, pure and mild, tasty and refreshing mouthfeel, the wine body quality of coordination and significant health-care efficacy.
Embodiment three
The present embodiment is related to the comparison of pueraria lobata cordyceps militaris liquor Yu existing pueraria lobata cordyceps militaris liquor quality of the invention.
Embodiment 3.1
The present embodiment is pueraria lobata cordyceps militaris liquor of the invention, using preparation method identical with embodiment 2.2, the pueraria lobata of acquisition
Chinese caterpillar fungus health wine, physical and chemical index and Analyses Methods for Sensory Evaluation Results are as shown in the table:
The alcoholic strength content and Flavonoid Content of the pueraria lobata cordyceps militaris liquor of acquisition are respectively 8.4%vol and 0.550g/L, are had
Significant health liquor effect.
Embodiment 3.2
The embodiment is comparative example, is mixed using by rice meal, malt flour and bean cake powder according to the mass ratio of 1:1:1
It closes, then adds the worm of identical with mixture (rice meal, malt flour and bean cake powder) total amount kudzu-vine root powder and kudzu-vine root powder weight 1/50
Grass meal, in mass ratio 1:4 add water to total raw material, and high pressure sterilization is simultaneously gelatinized, and are then cooled to 28 DEG C, by koji according to dry
The ratio of substance gross weight 0.4% adds to wherein, is saccharified 2 days in 28-30 DEG C, carries out according to 1% (w/w) ratio inoculation saccharomyces cerevisiae
Fermentation.After fermentation 20 days, cordyceps militaris liquor quality is measured, as a result as shown in the table:
By upper table result and 3.1 Comparative result of embodiment it is found that the cordyceps militaris liquor obtained by the method for embodiment 3.2 is in wine
Precision content is substantially less than pueraria lobata cordyceps militaris liquor of the invention in terms of Flavonoid Content, in addition, the cordyceps militaris liquor lack it is pure and mild, refreshing
The mouthfeel of mouth, Analyses Methods for Sensory Evaluation Results are also significantly lower than pueraria lobata cordyceps militaris liquor of the invention.
Embodiment 3.3
The embodiment is comparative experiments, (also known as cultivates mushroom bran using by kudzu-vine root powder and culture medium of Cordyceps militaris material, is fructification training
Support the remaining culture medium of cordyceps sinensis) it is mixed according to the mass ratio of 1:1, then add water, high pressure by the mass ratio of mixture and water 1:4
It sterilizes and is gelatinized, be then cooled to 28 DEG C, koji is added to wherein according to the ratio of dry matter gross weight 0.4%, in 28-
30 DEG C are saccharified 2 days, ferment according to 1% (w/w) ratio inoculation saccharomyces cerevisiae.After fermentation 20 days, cordyceps militaris liquor quality, knot are measured
Fruit is as shown in the table:
As seen from the above table, each index of cordyceps militaris liquor that the method for embodiment 3.3 obtains is substantially less than pueraria lobata cordyceps sinensis of the invention
Wine.
Comparative example 3.1 and 3.2,3.3 are as it can be seen that pueraria lobata cordyceps militaris liquor of the invention has good wine body quality and significant
Health-care efficacy, be a functional health wine best in quality.
Claims (6)
1. a kind of pueraria lobata cordyceps militaris liquor, it is characterised in that: mainly press the quality proportioning of 1:1 by puerarin saccharification liquid and Cordceps militaris fermentation liquid
Fermentation raw material is formed, brewing obtains pueraria lobata cordyceps militaris liquor；
Method includes the following steps:
A, it obtains puerarin saccharification liquid: taking kudzu-vine root powder, the distilled water of 2 times of kudzu-vine root powder volumes is added, mix, be gelatinized in 90-95 DEG C
Then ɑ-the amylase of 0.15%-0.3% (w/w) is added in 20min, after digesting 30-60min, cooling is ground by colloid mill
Cross 80 meshes afterwards, add the carbohydrase of 0.15%-0.3% (w/w), in 55-60 DEG C of heat preservation 1-2h after mixing, it is finally heated extremely
95 DEG C of enzyme deactivation 10-15min, obtain puerarin saccharification liquid；
B, it obtains Cordceps militaris fermentation liquid: b1, preparing Cordceps militaris seed liquor: being beaten with the punch that diameter is 6mm and take 4 Cordceps militaris bacterium
Kind, it is seeded in 100ml potato fluid nutrient medium, the shaking table culture 5 days under 25 DEG C, revolving speed 100r/min, obtains Cordceps militaris kind
Sub- liquid；
B2, it prepares Cordceps militaris fermentation liquid: the Cordceps militaris seed liquor of step b1 being seeded in Cordceps militaris fermentation culture, inoculum concentration
For 10% (v/v), shaking table culture 7 days under 25 DEG C of Yu Wendu, revolving speed 100r/min, Cordceps militaris fermentation liquid is obtained；
C, puerarin saccharification liquid is uniformly mixed with Cordceps militaris fermentation liquid by quality proportioning 1:1, is inoculated with the wine brewing ferment of 0.08% (w/w)
Mother is fermented at 25-30 DEG C, and after main fermentation 7-10 days, post-fermentation 10-20 days, centrifugal treating obtains pueraria lobata cordyceps militaris liquor；
It is mixed again with puerarin saccharification liquid after Cordceps militaris fermentation liquid described in step c is heat-treated, the heat treatment mode is
121 DEG C of high temperature high pressure process 20-30min.
2. pueraria lobata cordyceps militaris liquor according to claim 1, it is characterised in that: the total sugar content of the puerarin saccharification liquid is 200-
400g/L；The thallus weight in wet base of the Cordceps militaris fermentation liquid is 20-30g/L, cordycepin content is 98-160 μ g/L, cordyceps sinensis Thick many candies
Content is 4-6g/L.
3. pueraria lobata cordyceps militaris liquor according to claim 1, it is characterised in that: the alcoholic strength content of the pueraria lobata cordyceps militaris liquor is
6%-13%, flavones content 300-770mg/L, pH value 3.5-4.6.
4. a kind of preparation method of pueraria lobata cordyceps militaris liquor as described in claim 1, which is characterized in that method includes the following steps:
A, obtain puerarin saccharification liquid: by kudzu-vine root powder after gelatinization, ɑ-amylase enzymolysis, carbohydrase enzymatic hydrolysis, then destroy the enzyme treatment obtains
Puerarin saccharification liquid；
B, it obtains Cordceps militaris fermentation liquid: Cordceps militaris is inoculated in potato fluid nutrient medium, shaking table culture obtains Cordceps militaris seed
Liquid, then Cordceps militaris seed liquor is inoculated in Cordceps militaris fermentation culture, shaking table culture obtains Cordceps militaris fermentation liquid；
C, puerarin saccharification liquid is uniformly mixed with Cordceps militaris fermentation liquid by quality proportioning 1:1, is inoculated with the wine brewing ferment of 0.08% (w/w)
Mother is fermented at 25-30 DEG C, and after main fermentation 7-10 days, post-fermentation 10-20 days, centrifugal treating obtains pueraria lobata cordyceps militaris liquor.
5. the preparation method of pueraria lobata cordyceps militaris liquor according to claim 4, which is characterized in that the hair of Cordceps militaris described in step b
Ferment culture solution is prepared by the following steps:
Rice saccharified liquid is taken, the water of 2 times of rice saccharified liquid volumes is added, is uniformly mixed；Brewer's wort is taken, 0.5 times of brewer's wort is added
The water of volume is uniformly mixed；Dregs of beans juice is taken, the water of 1 times of dregs of beans juice volume is added, is uniformly mixed；By three kinds of solution according to 1:1:1
Volume ratio after mixing, in 121 DEG C of high pressure sterilization 15-20min, obtain Cordceps militaris fermentation culture.
6. the preparation method of pueraria lobata cordyceps militaris liquor according to claim 5, which is characterized in that
The rice saccharified liquid is prepared by the following steps: being taken rice meal, the water of 4 times of rice meal volumes is added, in 90-95 after mixing
DEG C gelatinization 30-60min after, be added 0.15-0.3% (w/w) ɑ-amylase, digest 30-60min, cooling, then cross colloid mill
Ground 80 mesh, adds the carbohydrase of 0.15-0.3% (w/w), in 55-60 DEG C of heat preservation 1-2h after mixing, it is finally heated extremely
95 DEG C of enzyme deactivation 10-15min, and it is centrifuged 15-20min under revolving speed 3000-4000r/min, obtained supernatant is rice saccharification
Liquid；
The brewer's wort is prepared by the following steps: taking malt flour, the water of 4 times of malt flour volumes is added, protect after mixing in 35-37 DEG C
Warm 30-60min, then at 50-55 DEG C of heat preservation 60-90min, finally after 60-65 DEG C of heat preservation 2.5h-3h, in revolving speed 3000-
It is centrifuged 10-15min under 4000r/min, obtained supernatant is brewer's wort；
The dregs of beans juice is prepared by the following steps: taking bean cake powder, the water of 10 times of bean cake powder volumes is added, protect after mixing in 100 DEG C
Warm 10-15min, is cooled to 55-60 DEG C, adds the papain of 0.15%-0.3% (w/w), mixes, and protects in 55-60 DEG C
After warm 1.5h-2h, it is centrifuged 10-15min at revolving speed 3000-4000r/min, obtained supernatant is dregs of beans juice.
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| CN101554121A (en) * | 2009-05-15 | 2009-10-14 | 江苏省农业科学院 | Fermentation method for high cordyceps militaris biomass and high cordycepin content |
| CN101693862A (en) * | 2009-10-19 | 2010-04-14 | 永州市雅大科技实业有限公司 | Kudzu-vine root and codyceps healthcare wine and brewing method thereof |
| CN103184125A (en) * | 2013-02-25 | 2013-07-03 | 廊坊师范学院 | Cordyceps militaris yellow wine made by two-step fermentation and preparation method thereof |
| CN105316206A (en) * | 2015-11-09 | 2016-02-10 | 福建农林大学 | Brewing method of pueraria lobata vinegar |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101554121A (en) * | 2009-05-15 | 2009-10-14 | 江苏省农业科学院 | Fermentation method for high cordyceps militaris biomass and high cordycepin content |
| CN101693862A (en) * | 2009-10-19 | 2010-04-14 | 永州市雅大科技实业有限公司 | Kudzu-vine root and codyceps healthcare wine and brewing method thereof |
| CN103184125A (en) * | 2013-02-25 | 2013-07-03 | 廊坊师范学院 | Cordyceps militaris yellow wine made by two-step fermentation and preparation method thereof |
| CN105316206A (en) * | 2015-11-09 | 2016-02-10 | 福建农林大学 | Brewing method of pueraria lobata vinegar |
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