CN105967770B - A kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw - Google Patents

A kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw Download PDF

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CN105967770B
CN105967770B CN201610271253.1A CN201610271253A CN105967770B CN 105967770 B CN105967770 B CN 105967770B CN 201610271253 A CN201610271253 A CN 201610271253A CN 105967770 B CN105967770 B CN 105967770B
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liquid
rice straw
fermentation
nutrient medium
fluid nutrient
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CN105967770A (en
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方华舟
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Jingchu University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

A kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw mixes rice straw fermentation infusion concentrate, Cordyceps militaris hypha fermentation infusion concentrate, the disease-resistant liquid equivalent of Chinese medicine insect prevention or inequality, as this organic Chinese medicine liquid fertilizer.Advantage: turning waste into wealth, and nutrient needed for crop is sufficient, and good absorbing absorbs rapidly, promotes metabolism, and with China's traditional Chinese medicine controlling disease, insect pest, health, ecology, environmental protection are rich in organic selenium, enhance the quality and healthcare function of crop.

Description

A kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw
Technical field
The present invention relates to agricultural fertilizer fields.
Technical background
For a long time, with the use of a large amount of pesticides, chemical fertilizer, causing the soil organism, there is a serious shortage of, harmful substance accumulation And not degradable, soil hardening, crop absorb nutrient difficulty, pest and disease damage is easily sent out multiple, drug resistance enhances, it has to lasting to increase Pesticide, fertilizer application amount reduce quality of agricultural product, endanger health of people, be also unfavorable for crop normal growth.Rapidly, it sufficiently mends It uses object organic nutrient as, meet crop to various nutrient demands, and with traditional Chinese medicine controlling disease insect pest, promote crop stalwartness raw Long, ecology, health, environmental protection to raising quality of agricultural product, long-term high yeild stable yields, guarantee health of people, significant.
Summary of the invention
The present invention is for there is a serious shortage of toxic to have for a large amount of applying pesticides in farmland, chemical fertilizer, the soil organism and nutrient for many years Evil substance residual, not degradable, the status that disease insect pest is easily sent out multiple, drug resistance enhances, agriculture chemical pollution is on the rise, and Invent a kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw, preparation method are as follows:
1) rice straw fermentation infusion concentrate is prepared
(1) rice straw fermenting microbe liquid is prepared
A bacterium culture medium) is prepared
A, it prepares A fluid nutrient medium: adding water 900-1100ml to be placed in potato 80-120g, rice straw powder 18-22g close It closes in container, heating maintains 110-130 DEG C, 0.5-1 hours after excluding cold air, adds brown sugar 4-6g after cooling, supplies water Amount mixes, 100 DEG C steam sterilizing 0.5-1 hour, obtained A fluid nutrient medium；
B, it prepares B fluid nutrient medium: adding water 900-1100ml to be placed in closed container rice straw powder 80-120g, add Heat maintains 110-130 DEG C, 0.5-1 hours after excluding cold air, adds (NH after cooling4)2SO4 2-3g、(NH4)3PO4 2-3g、 Supply water, mix, 100 DEG C steam sterilizing 0.5-1 hour, obtained B fluid nutrient medium；
C, C fluid nutrient medium is prepared: by brown sugar 38-42g, (NH4)2SO4 18-22g and water 900-110ml is mixed, and with Limewash adjusts pH value, pH value 6.5-7.5,100 DEG C steam sterilizing 0.5-1 hour, obtained C fluid nutrient medium；
D, it prepares D fluid nutrient medium: adding water 900-110ml to be placed in closed container rice straw powder 8-12g, heat 110-130 DEG C, 0.5-1 hours are maintained after excluding cold air, adds brown sugar 38-42g, (NH after cooling4)2SO4 18-22g, benefit Sufficient water mixes, and adjusts pH value with limewash, pH value 6.5-7.5,100 DEG C steam sterilizing 0.5-1 hour, obtained D liquid Culture medium；
B the strain liquid of each species strain) is prepared
A, Trichoderma viride strain is activated, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking tables of revolving speed Culture 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160-200r/min, 25-40 DEG C culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and mycelia Biomass (net value, dry weight subtracts dry weight before culture after culture) is up to 500 mg/100ml or more；
B, by aspergillus oryzae actication of culture, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking table trainings of revolving speed It supports 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160-200r/min, 25-40 DEG C culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and mycelia Biomass is up to 500 mg/100ml or more；
C, penicillium oxalicum strain is activated, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking tables of revolving speed Culture 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160-200r/min, 25-40 DEG C shaking table culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and Hypha biomass is up to 500 mg/100ml or more；
D, by delicious papulospora actication of culture, sterile access C fluid nutrient medium after activation, revolving speed 160-200r/min, 25-40 DEG C culture 4-5 days, until fermentation liquid is sticky, color change and visible mycelia raised growth；Continue sterile access D liquid training Base, 160-200r/min, 25-40 DEG C of revolving speed shaking table culture 4-5 days are supported, until fermentation liquid is sticky, color change, visible mycelia are a large amount of It grows and hypha biomass is up to 500 mg/100ml or more；
E, by brevibacterium flavum actication of culture, sterile access C fluid nutrient medium after activation, revolving speed 160-200r/min, 25-40 DEG C shaking table culture 1-2 days, until bacterium solution is muddy, color change；Continue sterile access D fluid nutrient medium, revolving speed 160- 200r/min, 25-40 DEG C shaking table culture 1-2 days, until fermentation liquid is muddy, color change, and smear through cell plates and to count up to 5 × 108/ ml or more；
C rice straw fermenting microbe liquid) is prepared
By the Trichoderma viride of above-mentioned culture, aspergillus oryzae, penicillium oxalicum, delicious papulospora and the strain liquid of brevibacterium flavum etc. Amount mixing, as rice straw fermenting microbe liquid；
(2) rice straw fermentation infusion concentrate is prepared
A) parts by weight of raw materials matches
100 brown sugar 3-5 kg of water 200-250 (NH of rice straw fermenting microbe liquid 15-20 rice straw4)2SO4 10-15 (NH4)3PO4 10-15 KH2PO4 5-10 MgCl2 5-10 FeSO4 3-5
MnCl2 3-5 ZnSO4 3-5 NaSeO3 0.5-1；
B) preparation method
A) rice straw ferments
Rice straw is smashed as 1-2cm segment, to pull out after the immersion of 2.8-3.2% limewash 12-24 hours, is put into hair In fermentation tank, while by the (NH of raw material proportioning addition water, brown sugar, rice straw fermenting microbe liquid and 1/2 formulation dosage4)2SO4 、 KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、NaSeO3, 30-40 DEG C, gas drive stirring fermentation 7-8 days, until in visible fermentor Mycelia raised growth；Continuously add another 1/2 dosage (NH4)2SO4 、KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、 NaSeO3, 30-40 DEG C, gas drive stirring fermentation 5-6 days, until visible a large amount of mycelia, stalk significantly reduce in fermentation liquid and corruption is soft, examines When surveying organic nitrogen content >=2.0g/100ml, i.e. completion rice straw fermentation；
B) infusion
After fermentation, fermentation liquid is transferred to airtight heating container, heating continues to be heated to 110- after excluding cold air 130 DEG C, stirring, infusion 20-24 hour, until stalk and mycelia are completely dissolved, as rice straw fermentation infusion liquid；
C) it is concentrated
100 kilograms of infusion liquid of rice straw fermentation are transferred to and is opened wide in heating container, continues mild fire and heats and remain micro- Boiling, stirring, infusion 2-4 hours, make liquid concentration to 30-35 kilograms, as rice straw fermentation infusion concentrate；
2) rice straw Cordyceps militaris mycelia infusion concentrate is prepared
A Cordyceps militaris spawn liquid) is prepared
A) it prepares E fluid nutrient medium: adding water 900-1100ml to boil to boiling peeled potatoes 80-120 g, dried silkworm chrysalis meal 8-12g Rise and continue mild fire and maintain boiling 0.5-1 hour, brown sugar 14-16g is added after cooling, rice straw ferment infusion liquid 80-120g, Supply water 1000ml, mix, be 6.0-6.5 with 1mol/L salt acid for adjusting pH value, 100 DEG C steam sterilizing 0.5-1 hour, it is obtained E fluid nutrient medium；
B) it prepares F fluid nutrient medium: rice straw fermentation infusion liquid 180-220g and water 900-1100ml is mixed, and Be 6.0-6.5 by the way that 1mol/L salt acid for adjusting pH value is added, 100 DEG C steam sterilizing 0.5-1 hour, obtained F fluid nutrient medium；
C) Cordyceps militaris spawn is activated, it is sterile to be linked into E fluid nutrient medium, 160-200r/min, 20-25 DEG C of shaking tables of revolving speed Culture 4-5 days, until fermentation liquid is sticky, color change and visible mycelia raised growth；
D) after mycelia raised growth, continue sterile access F fluid nutrient medium, 160-200r/min, 20-25 DEG C of revolving speed cultures 4-5 days, until fermentation liquid is sticky, color change, visible mycelia raised growth, hypha biomass reached 500mg/100ml or more, as Cordyceps militaris spawn liquid；
B rice straw Cordyceps militaris hypha fermentation infusion concentrate) is prepared
100 kilograms of rice straw fermentation infusion liquid are added in fermenting tank, it is public that Cordyceps militaris spawn liquid 4-6 is added Jin, 20-25 DEG C gas drive stir culture 5-7 day, until fermentation liquid is sticky, a large amount of mycelia occur and hypha biomass is up to 750-850mg/ 100ml or more terminates fermentation；After fermentation, fermentation liquid is transferred in airtight heating container, it is subsequent that heating excludes cold air It is continuous to be heated to 100-120 DEG C, stirring, infusion 3-4 hours, until mycelia is completely dissolved；After mycelia is completely dissolved, continue to be transferred to spacious It opens in heating container, mild fire heats and maintains micro-boiling 2-4 hours, makes liquid concentration to 30-35 kilograms, as rice straw pupa Chinese caterpillar fungus hypha fermentation infusion concentrate；
3) the disease-resistant pest-resistant liquid of Chinese medicine is prepared
Weigh kuh-seng, garden burnet, rhizome of cyrtomium, blackberry lily, great burdock achene, evodia rutaecarpa, folium artemisiae argyi, peppermint, purslane, field pennycress, dandelion Cauline leaf and root system, loguat leaf, Fructus Forsythiae fruit and cauline leaf, Pericarpium Zanthoxyli fruit and each 4-6 kilograms of blade add 2-3 times of water, are added together Into airtight heating container, heating continues to be heated to 100-120 DEG C after excluding cold air, and stirring, infusion 12-24 hours are filtered, Filtrate is transferred to and is opened wide in heating container, mild fire heats and maintain micro-boiling, stirring, infusion 3-4 hours, is concentrated into 40-50 Kilogram, the as disease-resistant pest-resistant liquid of Chinese medicine；
4) the organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw is prepared
Rice straw is fermented infusion concentrate, Cordyceps militaris mycelia infusion concentrate, the disease-resistant liquid equivalent of Chinese medicine insect prevention or not Equivalent mixes, as the organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw.
Advantage of the present invention
1, turn waste into wealth.The present invention follows ecological, environmental protective rule, using rice straw as primary raw material, passes through serial microorganism Effect, while sufficiently release rice straw nutrient, further synthesis sufficient protein, amino acid etc. is easy to by crop The various elements nutrition of a variety of organic nutrients and organic that absorb, it is ensured that demand of the crop to various nutrition；
2, the organic nutrient of rice straw is made full use of, fermenting and producing Cordyceps militaris mycelia simultaneously discharges wherein active constituent, promotees Into crop metabolism and disease resistance ability, promote crop strong sprout and development；
3, containing there are many local diseases preventions, pest-resistant, promotion crop metabolism Chinese herbal medicine has through many years compatibility mechanism in product Good diseases prevention is pest-resistant and growth promoting function, and can be used cooperatively to related pesticide etc., significantly improves drug effect, and easy to use；
5, this product is rich in organic selenium by microbial action, absorbs rapidly for crop, enhances application and makees The healthcare function of object；
6, this product is environmentally protective, can fill up the blank that quick-acting organic fertilizer is effectively combined with biological pesticide, is especially suitable for having Machine green agriculture field is widely popularized and uses.
Specific embodiment
This product it is best the preparation method comprises the following steps:
1) rice straw fermentation infusion concentrate is prepared
(1) rice straw fermenting microbe liquid is prepared
A bacterium culture medium) is prepared
A, it prepares A fluid nutrient medium: adding water 900-1100ml to be placed in potato 80-120g, rice straw powder 18-22g close It closes in container, heating maintains 110-130 DEG C, 0.5-1 hours after excluding cold air, adds brown sugar 4-6g after cooling, supplies water Amount mixes, 100 DEG C steam sterilizing 0.5-1 hour, obtained A fluid nutrient medium；
B, it prepares B fluid nutrient medium: adding water 900-1100ml to be placed in closed container rice straw powder 80-120g, add Heat maintains 110-130 DEG C, 0.5-1 hours after excluding cold air, adds (NH after cooling4)2SO4 2-3g、(NH4)3PO4 2-3g、 Supply water, mix, 100 DEG C steam sterilizing 0.5-1 hour, obtained B fluid nutrient medium；
C, C fluid nutrient medium is prepared: by brown sugar 38-42g, (NH4)2SO4 18-22g and water 900-110ml is mixed, and with Limewash adjusts pH value, pH value 6.5-7.5,100 DEG C steam sterilizing 0.5-1 hour, obtained C fluid nutrient medium；
D, it prepares D fluid nutrient medium: adding water 900-110ml to be placed in closed container rice straw powder 8-12g, heat 110-130 DEG C, 0.5-1 hours are maintained after excluding cold air, adds brown sugar 38-42g, (NH after cooling4)2SO4 18-22g, benefit Sufficient water mixes, and adjusts pH value with limewash, pH value 6.5-7.5,100 DEG C steam sterilizing 0.5-1 hour, obtained D liquid Culture medium；
B the strain liquid of each species strain) is prepared
A, Trichoderma viride strain is activated, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking tables of revolving speed Culture 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160-200r/min, 25-40 DEG C culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and mycelia Biomass (net value, dry weight subtracts dry weight before culture after culture) is up to 500 mg/100ml or more；
B, by aspergillus oryzae actication of culture, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking table trainings of revolving speed It supports 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160-200r/min, 25-40 DEG C culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and mycelia Biomass is up to 500 mg/100ml or more；
C, penicillium oxalicum strain is activated, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking tables of revolving speed Culture 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160-200r/min, 25-40 DEG C shaking table culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and Hypha biomass is up to 500 mg/100ml or more；
D, by delicious papulospora actication of culture, sterile access C fluid nutrient medium after activation, revolving speed 160-200r/min, 25-40 DEG C culture 4-5 days, until fermentation liquid is sticky, color change and visible mycelia raised growth；Continue sterile access D liquid training Base, 160-200r/min, 25-40 DEG C of revolving speed shaking table culture 4-5 days are supported, until fermentation liquid is sticky, color change, visible mycelia are a large amount of It grows and hypha biomass is up to 500 mg/100ml or more；
E, by brevibacterium flavum actication of culture, sterile access C fluid nutrient medium after activation, revolving speed 160-200r/min, 25-40 DEG C shaking table culture 1-2 days, until bacterium solution is muddy, color change；Continue sterile access D fluid nutrient medium, revolving speed 160- 200r/min, 25-40 DEG C shaking table culture 1-2 days, until fermentation liquid is muddy, color change, and smear through cell plates and to count up to 5 × 108/ ml or more；
C rice straw fermenting microbe liquid) is prepared
By the Trichoderma viride of above-mentioned culture, aspergillus oryzae, penicillium oxalicum, delicious papulospora and the strain liquid of brevibacterium flavum etc. Amount mixing, as rice straw fermenting microbe liquid；
(2) rice straw fermentation infusion concentrate is prepared
A) parts by weight of raw materials matches
100 brown sugar 3-5 kg of water 200-250 (NH of rice straw fermenting microbe liquid 15-20 rice straw4)2SO4 10-15 (NH4)3PO4 10-15 KH2PO4 5-10 MgCl2 5-10 FeSO4 3-5
MnCl2 3-5 ZnSO4 3-5 NaSeO3 0.5-1；
B) preparation method
A) rice straw ferments
Rice straw is smashed as 1-2cm segment, to pull out after the immersion of 2.8-3.2% limewash 12-24 hours, is put into hair In fermentation tank, while by the (NH of raw material proportioning addition water, brown sugar, rice straw fermenting microbe liquid and 1/2 formulation dosage4)2SO4 、 KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、NaSeO3, 30-40 DEG C, gas drive stirring fermentation 7-8 days, until in visible fermentor Mycelia raised growth；Continuously add another 1/2 dosage (NH4)2SO4 、KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、 NaSeO3, 30-40 DEG C, gas drive stirring fermentation 5-6 days, until visible a large amount of mycelia, stalk significantly reduce in fermentation liquid and corruption is soft, examines When surveying organic nitrogen content >=2.0g/100ml, i.e. completion rice straw fermentation；
B) infusion
After fermentation, fermentation liquid is transferred to airtight heating container, heating continues to be heated to 110- after excluding cold air 130 DEG C, stirring, infusion 20-24 hour, until stalk and mycelia are completely dissolved, as rice straw fermentation infusion liquid；
C) it is concentrated
100 kilograms of infusion liquid of rice straw fermentation are transferred to and is opened wide in heating container, continues mild fire and heats and remain micro- Boiling, stirring, infusion 2-4 hours, make liquid concentration to 30-35 kilograms, as rice straw fermentation infusion concentrate；
2) rice straw Cordyceps militaris mycelia infusion concentrate is prepared
A Cordyceps militaris spawn liquid) is prepared
A) it prepares E fluid nutrient medium: adding water 900-1100ml to boil to boiling peeled potatoes 80-120 g, dried silkworm chrysalis meal 8-12g Rise and continue mild fire and maintain boiling 0.5-1 hour, brown sugar 14-16g is added after cooling, rice straw ferment infusion liquid 80-120g, Supply water 1000ml, mix, be 6.0-6.5 with 1mol/L salt acid for adjusting pH value, 100 DEG C steam sterilizing 0.5-1 hour, it is obtained E fluid nutrient medium；
B) it prepares F fluid nutrient medium: rice straw fermentation infusion liquid 180-220g and water 900-1100ml is mixed, and Be 6.0-6.5 by the way that 1mol/L salt acid for adjusting pH value is added, 100 DEG C steam sterilizing 0.5-1 hour, obtained F fluid nutrient medium；
C) Cordyceps militaris spawn is activated, it is sterile to be linked into E fluid nutrient medium, 160-200r/min, 20-25 DEG C of shaking tables of revolving speed Culture 4-5 days, until fermentation liquid is sticky, color change and visible mycelia raised growth；
D) after mycelia raised growth, continue sterile access F fluid nutrient medium, 160-200r/min, 20-25 DEG C of revolving speed cultures 4-5 days, until fermentation liquid is sticky, color change, visible mycelia raised growth, hypha biomass reached 500mg/100ml or more, as Cordyceps militaris spawn liquid；
B rice straw Cordyceps militaris hypha fermentation infusion concentrate) is prepared
100 kilograms of rice straw fermentation infusion liquid are added in fermenting tank, it is public that Cordyceps militaris spawn liquid 4-6 is added Jin, 20-25 DEG C gas drive stir culture 5-7 day, until fermentation liquid is sticky, a large amount of mycelia occur and hypha biomass is up to 750-850mg/ 100ml or more terminates fermentation；After fermentation, fermentation liquid is transferred in airtight heating container, it is subsequent that heating excludes cold air It is continuous to be heated to 100-120 DEG C, stirring, infusion 3-4 hours, until mycelia is completely dissolved；After mycelia is completely dissolved, continue to be transferred to spacious It opens in heating container, mild fire heats and maintains micro-boiling 2-4 hours, makes liquid concentration to 30-35 kilograms, as rice straw pupa Chinese caterpillar fungus hypha fermentation infusion concentrate；
3) the disease-resistant pest-resistant liquid of Chinese medicine is prepared
Weigh kuh-seng, garden burnet, rhizome of cyrtomium, blackberry lily, great burdock achene, evodia rutaecarpa, folium artemisiae argyi, peppermint, purslane, field pennycress, dandelion Cauline leaf and root system, loguat leaf, Fructus Forsythiae fruit and cauline leaf, Pericarpium Zanthoxyli fruit and each 4-6 kilograms of blade add 2-3 times of water, are added together Into airtight heating container, heating continues to be heated to 100-120 DEG C after excluding cold air, and stirring, infusion 12-24 hours are filtered, Filtrate is transferred to and is opened wide in heating container, mild fire heats and maintain micro-boiling, stirring, infusion 3-4 hours, is concentrated into 40-50 Kilogram, the as disease-resistant pest-resistant liquid of Chinese medicine；
4) the organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw is prepared
Rice straw is fermented infusion concentrate, Cordyceps militaris mycelia infusion concentrate, the disease-resistant liquid equivalent of Chinese medicine insect prevention or not Equivalent mixes, as the organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw.
Strain source: Chinese agriculture Microbiological Culture Collection administrative center
Trichoderma viride (bacterial strain deposit number 30206), aspergillus oryzae (bacterial strain deposit number 31493) (bacterial strain deposit number 31493), grass Sour mould (bacterial strain deposit number 32576) (bacterial strain deposit number 32576), delicious papulospora (bacterial strain deposit number 30315), yellow quarter butt Bacterium (bacterial strain deposit number 11052), Cordyceps militaris (bacterial strain deposit number 50632).
Application method
This product is mainly used for crop top dressing and the prevention and control of plant diseases, pest control.It can occur just in the main growth phase of crop and pest and disease damage Phase carries out top dressing and the prevention and control of plant diseases, pest control, and amount of application is watered 20 times of progress foliage-sprays of dilution by 1000-2000ml per acre.It can also Top dressing is combined to carry out preventative application before crop disease and insect does not occur.When pest and disease damage is serious, it can be adjusted to related pesticide, growth Section agent etc. is mixed or is used cooperatively, and can suitably reduce extension rate.Chronic administration this product can improve soil texture and property Can, retain water and nutrients drought resisting and pest-resistant disease prevention ability are improved, crop yield and quality are improved, is especially suitable for the various crops such as rice Organic and green planting.

Claims (1)

1. a kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw, it is characterised in that the preparation method comprises the following steps:
1) rice straw fermentation infusion concentrate is prepared
(1) rice straw fermenting microbe liquid is prepared
A bacterium culture medium) is prepared
A, it prepares A fluid nutrient medium: adding water 900-1100ml to be placed in closed appearance potato 80-120g, rice straw powder 18-22g In device, heating maintains 110-130 DEG C, 0.5-1 hours after excluding cold air, adds brown sugar 4-6g after cooling, supplies water, mixes It is even, 100 DEG C steam sterilizing 0.5-1 hours, be made A fluid nutrient medium；
B, it prepares B fluid nutrient medium: adding water 900-1100ml to be placed in closed container rice straw powder 80-120g, heating row Except 110-130 DEG C, 0.5-1 hours is maintained after cold air, (NH is added after cooling4)2SO4 2-3g、(NH4)3PO4 2-3g, it supplies Water mixes, 100 DEG C steam sterilizing 0.5-1 hour, obtained B fluid nutrient medium；
C, C fluid nutrient medium is prepared: by brown sugar 38-42g, (NH4)2SO4 18-22g and water 900-110ml is mixed, and with lime Water adjusts pH value, pH value 6.5-7.5,100 DEG C steam sterilizing 0.5-1 hour, obtained C fluid nutrient medium；
D, it prepares D fluid nutrient medium: adding water 900-110ml to be placed in closed container rice straw powder 8-12g, heating excludes 110-130 DEG C, 0.5-1 hours are maintained after cold air, add brown sugar 38-42g, (NH after cooling4)2SO4 18-22g, water is supplied Amount mixes, and adjusts pH value with limewash, pH value 6.5-7.5,100 DEG C steam sterilizing 0.5-1 hour, obtained D Liquid Culture Base；
B the strain liquid of each species strain) is prepared
A, Trichoderma viride strain is activated, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking table cultures of revolving speed 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160- 200r/min, 25-40 DEG C culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and mycelia biology It measures up to 500 mg/100ml or more；
B, by aspergillus oryzae actication of culture, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking table culture 5- of revolving speed 7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160- 200r/min, 25-40 DEG C culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and mycelia biology It measures up to 500 mg/100ml or more；
C, penicillium oxalicum strain is activated, sterile access A fluid nutrient medium, 160-200r/min, 25-40 DEG C of shaking table cultures of revolving speed 5-7 days, until bacterium solution is sticky, color change and visible mycelia raised growth；Continue sterile access B fluid nutrient medium, revolving speed 160- 200r/min, 25-40 DEG C shaking table culture 7-8 days, until zymocyte liquid is sticky, color change, visible mycelia raised growth and mycelia Biomass is up to 500 mg/100ml or more；
D, by delicious papulospora actication of culture, sterile access C fluid nutrient medium, revolving speed 160-200r/min, 25-40 after activation DEG C culture 4-5 days, until fermentation liquid is sticky, color change and visible mycelia raised growth；Continue sterile access D fluid nutrient medium, 160-200r/min, 25-40 DEG C of revolving speed shaking table culture 4-5 days, until fermentation liquid is sticky, color change, visible mycelia raised growth And hypha biomass is up to 500 mg/100ml or more；
E, by brevibacterium flavum actication of culture, sterile access C fluid nutrient medium, revolving speed 160-200r/min, 25-40 after activation DEG C shaking table culture 1-2 days, until bacterium solution is muddy, color change；Continue sterile access D fluid nutrient medium, revolving speed 160-200r/min, 25-40 DEG C shaking table culture 1-2 days, until fermentation liquid is muddy, color change, and smears and counted up to 5 × 10 through cell plates8/ ml with On；
C rice straw fermenting microbe liquid) is prepared
The Trichoderma viride of above-mentioned culture, aspergillus oryzae, penicillium oxalicum, delicious papulospora and the strain liquid equivalent of brevibacterium flavum are mixed It closes, as rice straw fermenting microbe liquid；
(2) rice straw fermentation infusion concentrate is prepared
A) parts by weight of raw materials matches
100 brown sugar 3-5 kg of water 200-250 (NH of rice straw fermenting microbe liquid 15-20 rice straw4)2SO4 10-15 (NH4)3PO4 10-15 KH2PO4 5-10 MgCl2 5-10 FeSO4 3-5
MnCl2 3-5 ZnSO4 3-5 NaSeO3 0.5-1；
B) preparation method
A) rice straw ferments
Rice straw is smashed as 1-2cm segment, to pull out after the immersion of 2.8-3.2% limewash 12-24 hours, is put into fermentor In, while by the (NH of raw material proportioning addition water, brown sugar, rice straw fermenting microbe liquid and 1/2 formulation dosage4)2SO4 、 KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、NaSeO3, 30-40 DEG C, gas drive stirring fermentation 7-8 days, until in visible fermentor Mycelia raised growth；Continuously add another 1/2 dosage (NH4)2SO4 、KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、 NaSeO3, 30-40 DEG C, gas drive stirring fermentation 5-6 days, until visible a large amount of mycelia, stalk significantly reduce in fermentation liquid and corruption is soft, examines When surveying organic nitrogen content >=2.0g/100ml, i.e. completion rice straw fermentation；
B) infusion
After fermentation, fermentation liquid is transferred to airtight heating container, heating continues to be heated to 110-130 after excluding cold air DEG C, stirring, infusion 20-24 hour, until stalk and mycelia are completely dissolved, as rice straw fermentation infusion liquid；
C) it is concentrated
By rice straw fermentation 100 kilograms of infusion liquid be transferred to open wide heating container in, continue mild fire heat and maintain micro-boiling, Stirring, infusion 2-4 hours, make liquid concentration to 30-35 kilograms, as rice straw fermentation infusion concentrate；
2) rice straw Cordyceps militaris mycelia infusion concentrate is prepared
A Cordyceps militaris spawn liquid) is prepared
A) prepare E fluid nutrient medium: by peeled potatoes 80-120 g, dried silkworm chrysalis meal 8-12g add water 900-1100ml boil to boiling after Continuous mild fire maintains boiling 0.5-1 hours, and brown sugar 14-16g is added after cooling, rice straw fermentation infusion liquid 80-120g, is supplied Water 1000ml is mixed, and is 6.0-6.5 with 1mol/L salt acid for adjusting pH value, 100 DEG C steam sterilizing 0.5-1 hour, obtained E liquid Body culture medium；
B) it prepares F fluid nutrient medium: rice straw fermentation infusion liquid 180-220g and water 900-1100ml being mixed, and is passed through Addition 1mol/L salt acid for adjusting pH value is 6.0-6.5,100 DEG C steam sterilizing 0.5-1 hour, obtained F fluid nutrient medium；
C) Cordyceps militaris spawn is activated, it is sterile to be linked into E fluid nutrient medium, 160-200r/min, 20-25 DEG C of shaking table cultures of revolving speed 4-5 days, until fermentation liquid is sticky, color change and visible mycelia raised growth；
D) after mycelia raised growth, continue sterile access F fluid nutrient medium, 160-200r/min, 20-25 DEG C of culture 4-5 of revolving speed Day, until fermentation liquid is sticky, color change, visible mycelia raised growth, hypha biomass reaches 500mg/100ml or more, as pupa Cordyceps species liquid；
B rice straw Cordyceps militaris hypha fermentation infusion concentrate) is prepared
100 kilograms of rice straw fermentation infusion liquid are added in fermenting tank, 4-6 kilograms of Cordyceps militaris spawn liquid, 20- is added 25 DEG C gas drive stir culture 5-7 days, until fermentation liquid is sticky, a large amount of mycelia occur and hypha biomass reaches 750-850mg/100ml More than, terminate fermentation；After fermentation, fermentation liquid is transferred in airtight heating container, heating continues to add after excluding cold air Heat is to 100-120 DEG C, stirring, infusion 3-4 hours, until mycelia is completely dissolved；After mycelia is completely dissolved, continue to be transferred to unlimited add In heat container, mild fire heats and maintains micro-boiling 2-4 hours, makes liquid concentration to 30-35 kilograms, as rice straw Cordyceps militaris Hypha fermentation infusion concentrate；
3) the disease-resistant pest-resistant liquid of Chinese medicine is prepared
Weigh kuh-seng, garden burnet, rhizome of cyrtomium, blackberry lily, great burdock achene, evodia rutaecarpa, folium artemisiae argyi, peppermint, purslane, field pennycress, dandelion stem Leaf and root system, loguat leaf, Fructus Forsythiae fruit and cauline leaf, Pericarpium Zanthoxyli fruit and each 4-6 kilograms of blade add 2-3 times of water, are added to together close It closes in heating container, heating continues to be heated to 100-120 DEG C after excluding cold air, and stirring, infusion 12-24 hours, filtering will filter Liquid, which is transferred to, to be opened wide in heating container, and mild fire heats and maintain micro-boiling, stirring, infusion 3-4 hours, is concentrated into 40-50 kilograms, The as disease-resistant pest-resistant liquid of Chinese medicine；
4) the organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw is prepared
By rice straw fermentation infusion concentrate, Cordyceps militaris mycelia infusion concentrate, the disease-resistant liquid equivalent of Chinese medicine insect prevention or inequality It mixes, as the organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw.
CN201610271253.1A 2016-04-28 2016-04-28 A kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw Expired - Fee Related CN105967770B (en)

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CN102040408A (en) * 2010-06-25 2011-05-04 厦门三和利生物技术有限公司 Foliar fertilizer
CN104710232A (en) * 2013-12-16 2015-06-17 中国科学院沈阳应用生态研究所 Organic fertilizer prepared by use of cordyceps militaris waste medium and vitamin C fermentation waste gulonic acid mother liquor and preparation method thereof
CN105112343A (en) * 2015-09-28 2015-12-02 安徽飞天农用生物科技有限公司 Wheat straw decomposition agent and method for preparing same

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Publication number Priority date Publication date Assignee Title
CN102040408A (en) * 2010-06-25 2011-05-04 厦门三和利生物技术有限公司 Foliar fertilizer
CN104710232A (en) * 2013-12-16 2015-06-17 中国科学院沈阳应用生态研究所 Organic fertilizer prepared by use of cordyceps militaris waste medium and vitamin C fermentation waste gulonic acid mother liquor and preparation method thereof
CN105112343A (en) * 2015-09-28 2015-12-02 安徽飞天农用生物科技有限公司 Wheat straw decomposition agent and method for preparing same

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