CN105948959A - Rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer - Google Patents
Rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer Download PDFInfo
- Publication number
- CN105948959A CN105948959A CN201610271254.6A CN201610271254A CN105948959A CN 105948959 A CN105948959 A CN 105948959A CN 201610271254 A CN201610271254 A CN 201610271254A CN 105948959 A CN105948959 A CN 105948959A
- Authority
- CN
- China
- Prior art keywords
- rice straw
- liquid
- fluid medium
- fermentation
- prepared
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer. The rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer is prepared through uniformly mixing a rice-straw fermented and decocted concentrate, a Cordyceps militaris mycelium fermented and decocted concentrate, a rice-straw nitrogen fixation bacterial solution, a rice-straw phosphorus-dissolving potassium-dissolving bacterial solution and a rice-straw insect-resistant bacterial solution in an equal-volume or unequal-volume manner. The rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer has the advantages that the waste is changed into the valuable, the requirements of crops on various nutrients are met, and meanwhile, the fertilizer is rich in a large amount of functional microbes and organic selenium, so that the growing, metabolism and insect pest resisting capabilities of the crops are promoted, the self-accumulation of soil nutrients is enhanced, and the quality of the crops is improved.
Description
Technical field
The present invention relates to agricultural fertilizer field.
Technical background
In recent years, use chemical fertilizer, pesticide along with farmland in a large number, cause soil ecosystem heavy damage.Soil compaction,
Soil mineral salinity is high, the soil organism and organic nutrient famine, soil beneficial microbe are the most rare, and harmful substance amasss
Tired, Crop nutrient difficulty, pest-resistant resistance against diseases declines, Drug resistance strengthens, disease insect pest is easily sent out multiple, it has to greater amount
Use fertilizer and pesticide, formed vicious cycle, and caused quality of agricultural product to be remarkably decreased.Fully, supplement rapidly crop to can absorb
Organic nutrient, beneficial microbe etc., regulate and promote plant growth, rehabilitating soil ecosystem, gradually reduce or even do not make
With chemical fertilizer, pesticide, to improving soil function, strengthening soil nutrient oneself accumulation, raising quality of agricultural product and quality, extended high rate
Stable yields plays an important role.
Summary of the invention
The purpose of the present invention, is to use chemical fertilizer, pesticide in a large number for farmland in recent years, cause the soil organism and organic nutrient,
Fixing nitrogen, dissolving phosphor and dissolving potassium, pest-resistant anti-disease microbial famine, and soil compaction, Crop nutrient difficulty, soil ecology system
System heavy damage, chemical fertilizer, Pesticide use have become the present situation of vicious cycle, and it is pest-resistant to invent a kind of rice straw fixing nitrogen, dissolving phosphor and dissolving potassium
Selenium-rich organic microorganism liquid fertilizer, preparation method is:
1) rice straw fermentation infusion concentrated solution is prepared
(1) rice straw fermented bacterium liquid is prepared
A) bacterium culture medium is prepared
A, preparation A fluid medium: the 900-1100ml that Rhizoma Solani tuber osi 80-120g, rice straw powder 18-22g added water is placed in airtight appearance
In device, heating maintains 110-130 DEG C, 0.5-1 hour after getting rid of cold air, adds brown sugar 4-6g, supplies the water yield after cooling, mixed
Even, 100 DEG C of steam sterilizations 0.5-1 hour, prepare A fluid medium；
B, preparation B fluid medium: the 900-1100ml that added water by rice straw powder 80-120g is placed in hermetic container, adds hot type
Except maintaining 110-130 DEG C, 0.5-1 hour after cold air, after cooling, add (NH4)2SO4 2-3g、(NH4)3PO4 2-3g, supply
The water yield, mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare B fluid medium；
C, preparation C fluid medium: by brown sugar 38-42g, (NH4)2SO4 18-22g and water 900-110ml mixing, and with Calx
Water regulation pH value, pH value is 6.5-7.5,100 DEG C of steam sterilizations 0.5-1 hour, prepares C fluid medium；
D, preparation D fluid medium: the 900-110ml that added water by rice straw powder 8-12g is placed in hermetic container, and heating is got rid of
Maintain 110-130 DEG C, 0.5-1 hour after cold air, after cooling, add brown sugar 38-42g, (NH4)2SO4 18-22g, supply water
Amount, mixing, and regulate pH value with lime water, pH value is 6.5-7.5,100 DEG C of steam sterilizations 0.5-1 hour, prepares D liquid culture
Base；
B) strain liquid of each species strain is prepared
A, Trichoderma viride strain is activated, aseptic accesss A fluid medium, the cultivation of rotating speed 160-200r/min, 24-26 DEG C of shaking table
5-7 day, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
Cultivate 7-8 day for 200r/min, 24-26 DEG C, biological to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Amount (net value, dry weight before dry weight deducts cultivation after cultivation) reaches 500 more than mg/100ml；
B, by aspergillus oryzae actication of culture, aseptic access A fluid medium, 5-cultivated by rotating speed 160-200r/min, 24-26 DEG C of shaking table
7 days, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
Cultivate 7-8 day for 200r/min, 24-26 DEG C, biological to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Amount reaches 500 more than mg/100ml；
C, penicillium oxalicum strain is activated, aseptic accesss A fluid medium, the cultivation of rotating speed 160-200r/min, 24-26 DEG C of shaking table
5-7 day, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
200r/min, 24-26 DEG C of shaking table cultivates 7-8 day, to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Biomass reaches 500 more than mg/100ml；
D, by delicious food papulospora actication of culture, aseptic access C fluid medium, rotating speed 160-200r/min, 24-26 after activation
DEG C cultivate 4-5 day, to fermentation liquid thickness, color change visible mycelia raised growth；Continue aseptic access D fluid medium,
Rotating speed 160-200r/min, 24-26 DEG C of shaking table cultivates 4-5 day, to fermentation liquid thickness, color change, visible mycelia raised growth
And hypha biomass reaches 500 more than mg/100ml；
E, by brevibacterium flavum actication of culture, aseptic access C fluid medium, rotating speed 160-200r/min, 24-26 after activation
1-2 day cultivated by DEG C shaking table, color change muddy to bacterium solution；Continue aseptic access D fluid medium, rotating speed 160-200r/min,
1-2 day cultivated by 24-26 DEG C of shaking table, color change muddy to fermentation liquid, and smears counting through cell plates and reach 5 × 108/ ml with
On；
C) rice straw fermented bacterium liquid is prepared
The strain liquid equivalent of the Trichoderma viride of above-mentioned cultivation, aspergillus oryzae, penicillium oxalicum, delicious papulospora and brevibacterium flavum is mixed
Close, be rice straw fermented bacterium liquid；
(2) rice straw fermentation infusion concentrated solution is prepared
A) parts by weight of raw materials proportioning
Rice straw fermented bacterium liquid 15-20 rice straw 100 brown sugar 3-5 kg of water 200-250 (NH4)2SO4 10-15
(NH4)3PO4 10-15 KH2PO4 5-10 MgCl2 5-10 FeSO4 3-5
MnCl2 3-5 ZnSO4 3-5 NaSeO3 0.5-1；
B) preparation method
A) rice straw fermentation
Being smashed by rice straw is 1-2cm segment, pulls out, put into fermentation tank after soaking 12-24 hour with 2.8-3.2% lime water
In, add water, brown sugar, rice straw fermented bacterium liquid, the and (NH of 1/2 formula consumption by proportioning raw materials simultaneously4)2SO4 、
KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、NaSeO3, 30-40 DEG C, gas drive stirring fermentation 7-8 day, to visible fermentation tank
Mycelia raised growth；Continuously add the consumption (NH of another 1/24)2SO4 、KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、
NaSeO3, 30-40 DEG C, gas drive stirring fermentation 5-6 day, to fermentation liquid, visible a large amount of mycelia, straw significantly reduce and rotten soft, inspection
When surveying organic nitrogen content >=2.0g/100ml, i.e. complete rice straw fermentation；
B) infusion
After having fermented, fermentation liquid being transferred to the airtight heat container that adds, heating continues to be heated to 110-130 after getting rid of cold air
DEG C, stirring, infusion 20-24 hour, be completely dissolved to straw and mycelia, be rice straw fermentation infusion liquid；
C) concentrate
Infusion liquid 100 kilograms that rice straw is fermented be transferred to open wide add in heat container, continue slow fire heat and maintain micro-boiling,
Stirring, infusion 2-4 hour, make liquid concentration to 30-35 kilogram, be rice straw fermentation infusion concentrated solution；
2) rice straw Cordyceps militaris (L.) Link. mycelia infusion concentrated solution is prepared
A) Cordyceps militaris spawn liquid is prepared
A) E fluid medium is prepared: the 900-1100ml that peeled potatoes 80-120 g, dried silkworm chrysalis meal 8-12g added water boils to boiling and continues
Continuous slow fire maintains boiling 0.5-1 hour, adds brown sugar 14-16g, rice straw fermentation infusion liquid 80-120g, supplies after cooling
Water yield 1000ml, mixing, with 1mol/L salt acid for adjusting pH value as 6.0-6.5,100 DEG C of steam sterilizations 0.5-1 hour, prepare E liquid
Body culture medium；
B) F fluid medium is prepared: the infusion liquid 180-220g and water 900-1100ml that fermented by rice straw mixes, and passes through
Adding 1mol/L salt acid for adjusting pH value is 6.0-6.5, and 100 DEG C of steam sterilizations 0.5-1 hour prepare F fluid medium；
C) being activated by Cordyceps militaris spawn, the aseptic E fluid medium that is linked into, rotating speed 160-200r/min, 24-26 DEG C of shaking table is cultivated
4-5 day, to fermentation liquid thickness, color change visible mycelia raised growth；
D), after mycelia raised growth, continue aseptic access F fluid medium, cultivate 4-5 for rotating speed 160-200r/min, 24-26 DEG C
Day, to fermentation liquid thickness, color change, visible mycelia raised growth, hypha biomass reaches more than 500mg/100ml, is pupa
Cordyceps species liquid；
B) rice straw Cordyceps militaris (L.) Link. hypha fermentation infusion concentrated solution is prepared
100 kg of water rice straw fermentation infusion liquid are joined in fermenting tank, adds Cordyceps militaris spawn liquid 4-6 kilogram, 20-
25 DEG C of gas drive stir culture 5-7 days, to fermentation liquid thickness, occur that a large amount of mycelia and hypha biomass reach 750-850mg/100ml
Above, fermentation is terminated；After having fermented, being transferred to fermentation liquid airtight add in heat container, heating continues to add after getting rid of cold air
Hot to 100-120 DEG C, stirring, infusion 3-4 hour, it is completely dissolved to mycelia；After mycelia is completely dissolved, continue to be transferred to open wide to add
In heat container, slow fire heats and maintains micro-boiling 2-4 hour, makes liquid concentration to 30-35 kilogram, is rice straw Cordyceps militaris (L.) Link.
Hypha fermentation infusion concentrated solution；
3) rice straw fixed nitrogen bacterium solution is prepared
A) G fluid medium is prepared
By peptone 4-6g, rice straw fermentation infusion liquid 80-120g, water 900-1100ml mixing, 100 DEG C of steam sterilization 0.5-
1 hour, prepare G fluid medium；
B) H fluid medium is prepared
Rice straw is fermented infusion liquid 480-520g, water 900-1100ml mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare
H fluid medium；
C) rice straw fixed nitrogen strain liquid is prepared
By Bacillus cereus, A Shi bacillus cereus, activate addicted to sugar Pseudomonas alba, nitrogen-fixing bacteria strain, the most aseptic connect
Entering G fluid medium, 1-2 day cultivated by rotating speed 160-200r/min, 25-35 DEG C of shaking table, muddy to bacterium solution, continues aseptic access H
Fluid medium, 1-2 day cultivated by 160-200r/min, 25-35 DEG C of shaking table, muddy to each species strain bacterium solution, and puts down through cell
Plate counting reaches 5 × 108/ more than ml, is each bacterial strain strain liquid；Again by each bacterial strain strain liquid mixed in equal amounts, it is rice straw
Fixed nitrogen strain liquid；
D) rice straw fixed nitrogen bacterium solution is prepared
The infusion liquid 100 kilograms that fermented by rice straw joins in fermenting tank, and adds rice straw fixed nitrogen strain liquid 4-6
Kilogram, gas drive stirring fermentation, cultivate 1-2 day, reach 5 × 10 to cell counting for 25-40 DEG C8/ more than ml, is rice straw fixed nitrogen
Bacterium solution；
4) rice straw dissolving phosphor and dissolving potassium bacterium solution is prepared
A) I fluid medium is prepared
By peptone 4-6 g, rice straw fermentation infusion liquid 80-120g, ground phosphate rock 8-12g, feldspar in powder 4-6g, water 900-
1100ml mixes, 100 DEG C of steam sterilizations 0.5-1 hour, prepares I fluid medium；
B) J fluid medium is prepared
Rice straw is fermented infusion liquid 480-520g, ground phosphate rock 8-12g, feldspar in powder 4-6g, water 900-1100ml mixing,
100 DEG C of steam sterilizations 0.5-1 hour, prepare J fluid medium；
C) dissolving phosphor and dissolving potassium bacterial strain strain liquid is prepared
Bacillus subtillis, bacillus cereus, colloid bacillus cereus are activated, respectively aseptic access I fluid medium, turn
1-2 day cultivated by 160-200r/min, 25-35 DEG C of shaking table of speed, when each bacterial strain raised growth, bacterium solution muddiness, the most aseptic
Accessing J fluid medium, 1-2 day cultivated by rotating speed 160-200r/min, 25-35 DEG C of shaking table, cell muddy to each bacterial strain bacterium solution
Counting reaches 5 × 108/ more than ml, is each dissolving phosphor and dissolving potassium bacterial strain strain liquid；
D) rice straw dissolving phosphor and dissolving potassium bacterium solution is prepared
A) proportioning raw materials
Rice straw fermentation infusion liquid 80-120 kilogram, 1-3 kilogram of ground phosphate rock, feldspar in powder 0.5-2 kilogram；
B) preparation method
In each fermenting tank, add rice straw fermentation infusion liquid, ground phosphate rock, feldspar in powder according to formula, then add respectively
Enter each dissolving phosphor and dissolving potassium bacterial strain strain liquid 4-6 kilogram, and gas drive stirring, 25-40 DEG C of fermentation 8-10 day, reach to each strain cell counting
5×108/ more than ml, is each corresponding bacterial strain rice straw dissolving phosphor and dissolving potassium bacterium solution；By each bacterial strain bacterium solution mixed in equal amounts, it is water
Rice straw dissolving phosphor and dissolving potassium bacterium solution；
5) the pest-resistant bacterium solution of rice straw is prepared
A) pest-resistant strain liquid is prepared
A, preparation K fluid medium
By peptone 4-6 g, rice straw fermentation infusion liquid 80-120g, water 900-1100ml mixing, 100 DEG C of steam sterilizations
0.5-1 hour, prepare K fluid medium；
B, preparation L fluid medium
Rice straw fermentation infusion liquid 480-520g, water 900-1100ml mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare L
Fluid medium；
C, being activated by bacillus thuringiensis, aseptic access K fluid medium, rotating speed 160-200r/min, 25-35 DEG C shake
1-2 day cultivated by bed, muddy to bacterium solution；Continue aseptic access L fluid medium, rotating speed 160-200r/min, 25-35 DEG C of shaking table training
Support 1-2 day, to bacterium solution is muddy and cell counting reaches 5 × 108/ more than ml, is pest-resistant strain liquid；
B) the pest-resistant bacterium solution of rice straw is prepared
In fermenting tank, add rice straw fermentation infusion liquid 100 kilograms and pest-resistant strain liquid 4-6 kilogram, 25-35 DEG C of gas
Drive stirring fermentation 1-2 day, reach 1 × 10 to bacterial strain raised growth, plate count9/ more than ml, is the pest-resistant bacterium solution of rice straw；
6) rice straw fixing nitrogen, dissolving phosphor and dissolving potassium pest-resistant organic microbial liquid fertilizer is prepared
Rice straw is fermented infusion concentrated solution, Cordyceps militaris (L.) Link. mycelia infusion concentrated solution, rice straw fixed nitrogen bacterium solution, rice straw solution
Phosphorus potassium decomposing bacterium solution, rice straw pest-resistant bacterium solution equivalent or inequality mixing, be the pest-resistant selenium-rich of rice straw fixing nitrogen, dissolving phosphor and dissolving potassium
Organic microbial liquid fertilizer.
Advantage of the present invention
1, turn waste into wealth.The present invention follows ecological, environmental protective rule, with rice straw as primary raw material, by the work of series microorganism
With, while fully release rice straw nutrient, the protein of synthesis abundance further, aminoacid etc. are prone to absorbed by crops
Various organic and element nutrition, it is ensured that the various nutrition of crop and element demand；
2, making full use of the organic nutrient of rice straw, fermenting and producing Cordyceps militaris (L.) Link. mycelia also discharges wherein active component, promotes to make
Thing metabolism and disease resistance ability, promote crop strong sprout and growth；
3, according to conspiracy relation between bacterial strain in formula, this product compatibility constitutes the function bacterium such as fixing nitrogen, dissolving phosphor and dissolving potassium, parasite killing,
And the product content of organic matter enrich, fully meet crop to various nutrient demands while, also can effectively ensure that each function is micro-
Biology plays a role rapidly after applying farmland, constantly repairs and improves soil function, strengthens soil oneself's nutrient accumulation and insect protected
Diseases prevention ability, can prevent and treat the multiple harmful insects such as Lepidoptera, Orthoptera, coleoptera, Diptera, Hymenoptera；
4, this product is by microbial action rich in organic selenium element, is available for crop and absorbs rapidly, enhances and use crop
Health care；
5, through the test of local relevant department, use this product and typically can increase production 3-10% and significantly improve quality of agricultural product, alleviate worm
Disease, is especially suitable for green organic agriculture field and is widely popularized and uses.
Detailed description of the invention
The optimal preparation method of this product is:
1) rice straw fermentation infusion concentrated solution is prepared
(1) rice straw fermented bacterium liquid is prepared
A) bacterium culture medium is prepared
A, preparation A fluid medium: the 900-1100ml that Rhizoma Solani tuber osi 80-120g, rice straw powder 18-22g added water is placed in airtight appearance
In device, heating maintains 110-130 DEG C, 0.5-1 hour after getting rid of cold air, adds brown sugar 4-6g, supplies the water yield after cooling, mixed
Even, 100 DEG C of steam sterilizations 0.5-1 hour, prepare A fluid medium；
B, preparation B fluid medium: the 900-1100ml that added water by rice straw powder 80-120g is placed in hermetic container, adds hot type
Except maintaining 110-130 DEG C, 0.5-1 hour after cold air, after cooling, add (NH4)2SO4 2-3g、(NH4)3PO4 2-3g, supply
The water yield, mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare B fluid medium；
C, preparation C fluid medium: by brown sugar 38-42g, (NH4)2SO4 18-22g and water 900-110ml mixing, and with Calx
Water regulation pH value, pH value is 6.5-7.5,100 DEG C of steam sterilizations 0.5-1 hour, prepares C fluid medium；
D, preparation D fluid medium: the 900-110ml that added water by rice straw powder 8-12g is placed in hermetic container, and heating is got rid of
Maintain 110-130 DEG C, 0.5-1 hour after cold air, after cooling, add brown sugar 38-42g, (NH4)2SO4 18-22g, supply water
Amount, mixing, and regulate pH value with lime water, pH value is 6.5-7.5,100 DEG C of steam sterilizations 0.5-1 hour, prepares D liquid culture
Base；
B) strain liquid of each species strain is prepared
A, Trichoderma viride strain is activated, aseptic accesss A fluid medium, the cultivation of rotating speed 160-200r/min, 24-26 DEG C of shaking table
5-7 day, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
Cultivate 7-8 day for 200r/min, 24-26 DEG C, biological to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Amount (net value, dry weight before dry weight deducts cultivation after cultivation) reaches 500 more than mg/100ml；
B, by aspergillus oryzae actication of culture, aseptic access A fluid medium, 5-cultivated by rotating speed 160-200r/min, 24-26 DEG C of shaking table
7 days, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
Cultivate 7-8 day for 200r/min, 24-26 DEG C, biological to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Amount reaches 500 more than mg/100ml；
C, penicillium oxalicum strain is activated, aseptic accesss A fluid medium, the cultivation of rotating speed 160-200r/min, 24-26 DEG C of shaking table
5-7 day, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
200r/min, 24-26 DEG C of shaking table cultivates 7-8 day, to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Biomass reaches 500 more than mg/100ml；
D, by delicious food papulospora actication of culture, aseptic access C fluid medium, rotating speed 160-200r/min, 24-26 after activation
DEG C cultivate 4-5 day, to fermentation liquid thickness, color change visible mycelia raised growth；Continue aseptic access D fluid medium,
Rotating speed 160-200r/min, 24-26 DEG C of shaking table cultivates 4-5 day, to fermentation liquid thickness, color change, visible mycelia raised growth
And hypha biomass reaches 500 more than mg/100ml；
E, by brevibacterium flavum actication of culture, aseptic access C fluid medium, rotating speed 160-200r/min, 24-26 after activation
1-2 day cultivated by DEG C shaking table, color change muddy to bacterium solution；Continue aseptic access D fluid medium, rotating speed 160-200r/min,
1-2 day cultivated by 24-26 DEG C of shaking table, color change muddy to fermentation liquid, and smears counting through cell plates and reach 5 × 108/ ml with
On；
C) rice straw fermented bacterium liquid is prepared
The strain liquid equivalent of the Trichoderma viride of above-mentioned cultivation, aspergillus oryzae, penicillium oxalicum, delicious papulospora and brevibacterium flavum is mixed
Close, be rice straw fermented bacterium liquid；
(2) rice straw fermentation infusion concentrated solution is prepared
A) parts by weight of raw materials proportioning
Rice straw fermented bacterium liquid 15-20 rice straw 100 brown sugar 3-5 kg of water 200-250 (NH4)2SO4 10-15
(NH4)3PO4 10-15 KH2PO4 5-10 MgCl2 5-10 FeSO4 3-5
MnCl2 3-5 ZnSO4 3-5 NaSeO3 0.5-1；
B) preparation method
A) rice straw fermentation
Being smashed by rice straw is 1-2cm segment, pulls out, put into fermentation tank after soaking 12-24 hour with 2.8-3.2% lime water
In, add water, brown sugar, rice straw fermented bacterium liquid, the and (NH of 1/2 formula consumption by proportioning raw materials simultaneously4)2SO4 、
KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、NaSeO3, 30-40 DEG C, gas drive stirring fermentation 7-8 day, to visible fermentation tank
Mycelia raised growth；Continuously add the consumption (NH of another 1/24)2SO4 、KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、
NaSeO3, 30-40 DEG C, gas drive stirring fermentation 5-6 day, to fermentation liquid, visible a large amount of mycelia, straw significantly reduce and rotten soft, inspection
When surveying organic nitrogen content >=2.0g/100ml, i.e. complete rice straw fermentation；
B) infusion
After having fermented, fermentation liquid being transferred to the airtight heat container that adds, heating continues to be heated to 110-130 after getting rid of cold air
DEG C, stirring, infusion 20-24 hour, be completely dissolved to straw and mycelia, be rice straw fermentation infusion liquid；
C) concentrate
Infusion liquid 100 kilograms that rice straw is fermented be transferred to open wide add in heat container, continue slow fire heat and maintain micro-boiling,
Stirring, infusion 2-4 hour, make liquid concentration to 30-35 kilogram, be rice straw fermentation infusion concentrated solution；
2) rice straw Cordyceps militaris (L.) Link. mycelia infusion concentrated solution is prepared
A) Cordyceps militaris spawn liquid is prepared
A) E fluid medium is prepared: the 900-1100ml that peeled potatoes 80-120 g, dried silkworm chrysalis meal 8-12g added water boils to boiling and continues
Continuous slow fire maintains boiling 0.5-1 hour, adds brown sugar 14-16g, rice straw fermentation infusion liquid 80-120g, supplies after cooling
Water yield 1000ml, mixing, with 1mol/L salt acid for adjusting pH value as 6.0-6.5,100 DEG C of steam sterilizations 0.5-1 hour, prepare E liquid
Body culture medium；
B) F fluid medium is prepared: the infusion liquid 180-220g and water 900-1100ml that fermented by rice straw mixes, and passes through
Adding 1mol/L salt acid for adjusting pH value is 6.0-6.5, and 100 DEG C of steam sterilizations 0.5-1 hour prepare F fluid medium；
C) being activated by Cordyceps militaris spawn, the aseptic E fluid medium that is linked into, rotating speed 160-200r/min, 20-25 DEG C of shaking table is cultivated
4-5 day, to fermentation liquid thickness, color change visible mycelia raised growth；
D), after mycelia raised growth, continue aseptic access F fluid medium, cultivate 4-5 for rotating speed 160-200r/min, 20-25 DEG C
Day, to fermentation liquid thickness, color change, visible mycelia raised growth, hypha biomass reaches more than 500mg/100ml, is pupa
Cordyceps species liquid；
B) rice straw Cordyceps militaris (L.) Link. hypha fermentation infusion concentrated solution is prepared
100 kg of water rice straw fermentation infusion liquid are joined in fermenting tank, adds Cordyceps militaris spawn liquid 4-6 kilogram, 20-
25 DEG C of gas drive stir culture 5-7 days, to fermentation liquid thickness, occur that a large amount of mycelia and hypha biomass reach 750-850mg/100ml
Above, fermentation is terminated；After having fermented, being transferred to fermentation liquid airtight add in heat container, heating continues to add after getting rid of cold air
Hot to 100-120 DEG C, stirring, infusion 3-4 hour, it is completely dissolved to mycelia；After mycelia is completely dissolved, continue to be transferred to open wide to add
In heat container, slow fire heats and maintains micro-boiling 2-4 hour, makes liquid concentration to 30-35 kilogram, is rice straw Cordyceps militaris (L.) Link.
Hypha fermentation infusion concentrated solution；
3) rice straw fixed nitrogen bacterium solution is prepared
A) G fluid medium is prepared
By peptone 4-6g, rice straw fermentation infusion liquid 80-120g, water 900-1100ml mixing, 100 DEG C of steam sterilization 0.5-
1 hour, prepare G fluid medium；
B) H fluid medium is prepared
Rice straw is fermented infusion liquid 480-520g, water 900-1100ml mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare
H fluid medium；
C) rice straw fixed nitrogen strain liquid is prepared
By Bacillus cereus, A Shi bacillus cereus, activate addicted to sugar Pseudomonas alba, nitrogen-fixing bacteria strain, the most aseptic connect
Entering G fluid medium, 1-2 day cultivated by rotating speed 160-200r/min, 25-35 DEG C of shaking table, muddy to bacterium solution, continues aseptic access H
Fluid medium, 1-2 day cultivated by 160-200r/min, 25-35 DEG C of shaking table, muddy to each species strain bacterium solution, and puts down through cell
Plate counting reaches 5 × 108/ more than ml, is each bacterial strain strain liquid；Again by each bacterial strain strain liquid mixed in equal amounts, it is rice straw
Fixed nitrogen strain liquid；
D) rice straw fixed nitrogen bacterium solution is prepared
The infusion liquid 100 kilograms that fermented by rice straw joins in fermenting tank, and adds rice straw fixed nitrogen strain liquid 4-6
Kilogram, gas drive stirring fermentation, cultivate 1-2 day, reach 5 × 10 to cell counting for 25-40 DEG C8/ more than ml, is rice straw fixed nitrogen
Bacterium solution；
4) rice straw dissolving phosphor and dissolving potassium bacterium solution is prepared
A) I fluid medium is prepared
By peptone 4-6 g, rice straw fermentation infusion liquid 80-120g, ground phosphate rock 8-12g, feldspar in powder 4-6g, water 900-
1100ml mixes, 100 DEG C of steam sterilizations 0.5-1 hour, prepares I fluid medium；
B) J fluid medium is prepared
Rice straw is fermented infusion liquid 480-520g, ground phosphate rock 8-12g, feldspar in powder 4-6g, water 900-1100ml mixing,
100 DEG C of steam sterilizations 0.5-1 hour, prepare J fluid medium；
C) dissolving phosphor and dissolving potassium bacterial strain strain liquid is prepared
Bacillus subtillis, bacillus cereus, colloid bacillus cereus are activated, respectively aseptic access I fluid medium, turn
1-2 day cultivated by 160-200r/min, 25-35 DEG C of shaking table of speed, when each bacterial strain raised growth, bacterium solution muddiness, the most aseptic
Accessing J fluid medium, 1-2 day cultivated by rotating speed 160-200r/min, 25-35 DEG C of shaking table, cell muddy to each bacterial strain bacterium solution
Counting reaches 5 × 108/ more than ml, is each dissolving phosphor and dissolving potassium bacterial strain strain liquid；
D) rice straw dissolving phosphor and dissolving potassium bacterium solution is prepared
A) proportioning raw materials
Rice straw fermentation infusion liquid 80-120 kilogram, 1-3 kilogram of ground phosphate rock, feldspar in powder 0.5-2 kilogram；
B) preparation method
In each fermenting tank, add rice straw fermentation infusion liquid, ground phosphate rock, feldspar in powder according to formula, then add respectively
Enter each dissolving phosphor and dissolving potassium bacterial strain strain liquid 4-6 kilogram, and gas drive stirring, 25-40 DEG C of fermentation 8-10 day, reach to each strain cell counting
5×108/ more than ml, is each corresponding bacterial strain rice straw dissolving phosphor and dissolving potassium bacterium solution；By each bacterial strain bacterium solution mixed in equal amounts, it is water
Rice straw dissolving phosphor and dissolving potassium bacterium solution；
5) the pest-resistant bacterium solution of rice straw is prepared
A) pest-resistant strain liquid is prepared
A, preparation K fluid medium
By peptone 4-6 g, rice straw fermentation infusion liquid 80-120g, water 900-1100ml mixing, 100 DEG C of steam sterilizations
0.5-1 hour, prepare K fluid medium；
B, preparation L fluid medium
Rice straw fermentation infusion liquid 480-520g, water 900-1100ml mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare L
Fluid medium；
C, being activated by bacillus thuringiensis, aseptic access K fluid medium, rotating speed 160-200r/min, 25-35 DEG C shake
1-2 day cultivated by bed, muddy to bacterium solution；Continue aseptic access L fluid medium, rotating speed 160-200r/min, 25-35 DEG C of shaking table training
Support 1-2 day, to bacterium solution is muddy and cell counting reaches 5 × 108/ more than ml, is pest-resistant strain liquid；
B) the pest-resistant bacterium solution of rice straw is prepared
In fermenting tank, add rice straw fermentation infusion liquid 100 kilograms and pest-resistant strain liquid 4-6 kilogram, 25-35 DEG C of gas
Drive stirring fermentation 1-2 day, reach 1 × 10 to bacterial strain raised growth, plate count9/ more than ml, is the pest-resistant bacterium solution of rice straw；
6) rice straw fixing nitrogen, dissolving phosphor and dissolving potassium pest-resistant organic microbial liquid fertilizer is prepared
Rice straw is fermented infusion concentrated solution, Cordyceps militaris (L.) Link. mycelia infusion concentrated solution, rice straw fixed nitrogen bacterium solution, rice straw solution
Phosphorus potassium decomposing bacterium solution, rice straw pest-resistant bacterium solution equivalent or inequality mixing, be the pest-resistant selenium-rich of rice straw fixing nitrogen, dissolving phosphor and dissolving potassium
Organic microbial liquid fertilizer.
Strain is originated: Chinese agriculture Microbiological Culture Collection administrative center
Trichoderma viride (bacterial strain preserving number 30206), aspergillus oryzae (bacterial strain preserving number 31493) (bacterial strain preserving number 31493), oxalic acid are blue or green
Mould (bacterial strain preserving number 32576) (bacterial strain preserving number 32576), delicious papulospora (bacterial strain preserving number 30315), brevibacterium flavum
(bacterial strain preserving number 11052), Cordyceps militaris (L.) Link. (bacterial strain preserving number 50632), Bacillus cereus (bacterial strain preserving number 19428), A Shi
Bacillus cereus (bacterial strain preserving number 06384), addicted to sugar Pseudomonas alba (bacterial strain preserving number 01133), nitrogen-fixing bacteria (bacterial strain preserving number
01437), Bacillus subtillis (bacterial strain preserving number 19743), bacillus cereus (bacterial strain preserving number 01467), colloid bacillus cereus
(bacterial strain preserving number 19749).
Using method
This product is mainly used in crop and topdresses and the prevention and control of plant diseases, pest control.Can enter in crop primary growth stage and pest and disease damage early period of origination
Row topdresses and the prevention and control of plant diseases, pest control, and amount of application presses every mu of 1000-2000ml, is watered dilution 20 times and carries out foliage-spray.Also can make
Thing pest and disease damage does not occurs to combine before to topdress and carries out preventative using.Can not simultaneously spraying pesticide when this product is used, it is to avoid strong
The extreme weather conditions such as direct sunlight, rainfall, high temperature are used, and before and after using this product within about one week, with not applying pesticides are
Preferably.To serious pest and disease damage, after spraying pesticide 5-7 days, this product can be used to consolidate pest and disease damage on the basis of conventional pesticide is prevented and treated
Prevention effect (can suitably reduce extension rate).This product of chronic administration, can progressively improve Soil structure and performance, rehabilitating soil
Ecosystem, improves soil water-reataining fertilizer-keeping drought resisting and pest-resistant diseases prevention ability, improves quality of agricultural product and quality, is especially suitable for Oryza sativa L.
Etc. the organic of various crops and green planting.
Claims (1)
1. a rice straw fixing nitrogen, dissolving phosphor and dissolving potassium pest-resistant selenium-rich organic microorganism liquid fertilizer, it is characterised in that preparation method is:
1) rice straw fermentation infusion concentrated solution is prepared
(1) rice straw fermented bacterium liquid is prepared
A) bacterium culture medium is prepared
A, preparation A fluid medium: the 900-1100ml that Rhizoma Solani tuber osi 80-120g, rice straw powder 18-22g added water is placed in airtight appearance
In device, heating maintains 110-130 DEG C, 0.5-1 hour after getting rid of cold air, adds brown sugar 4-6g, supplies the water yield after cooling, mixed
Even, 100 DEG C of steam sterilizations 0.5-1 hour, prepare A fluid medium；
B, preparation B fluid medium: the 900-1100ml that added water by rice straw powder 80-120g is placed in hermetic container, adds hot type
Except maintaining 110-130 DEG C, 0.5-1 hour after cold air, after cooling, add (NH4)2SO4 2-3g、(NH4)3PO4 2-3g, supply
The water yield, mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare B fluid medium；
C, preparation C fluid medium: by brown sugar 38-42g, (NH4)2SO4 18-22g and water 900-110ml mixing, and with Calx
Water regulation pH value, pH value is 6.5-7.5,100 DEG C of steam sterilizations 0.5-1 hour, prepares C fluid medium；
D, preparation D fluid medium: the 900-110ml that added water by rice straw powder 8-12g is placed in hermetic container, and heating is got rid of
Maintain 110-130 DEG C, 0.5-1 hour after cold air, after cooling, add brown sugar 38-42g, (NH4)2SO4 18-22g, supply water
Amount, mixing, and regulate pH value with lime water, pH value is 6.5-7.5,100 DEG C of steam sterilizations 0.5-1 hour, prepares D liquid culture
Base；
B) strain liquid of each species strain is prepared
A, Trichoderma viride strain is activated, aseptic accesss A fluid medium, the cultivation of rotating speed 160-200r/min, 25-40 DEG C of shaking table
5-7 day, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
Cultivate 7-8 day for 200r/min, 25-40 DEG C, biological to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Amount reaches 500 more than mg/100ml；
B, by aspergillus oryzae actication of culture, aseptic access A fluid medium, 5-cultivated by rotating speed 160-200r/min, 25-40 DEG C of shaking table
7 days, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
Cultivate 7-8 day for 200r/min, 25-40 DEG C, biological to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Amount reaches 500 more than mg/100ml；
C, penicillium oxalicum strain is activated, aseptic accesss A fluid medium, the cultivation of rotating speed 160-200r/min, 25-40 DEG C of shaking table
5-7 day, to bacterium solution thickness, color change visible mycelia raised growth；Continue aseptic access B fluid medium, rotating speed 160-
200r/min, 25-40 DEG C of shaking table cultivates 7-8 day, to zymocyte liquid thickness, color change, visible mycelia raised growth and mycelia
Biomass reaches 500 more than mg/100ml；
D, by delicious food papulospora actication of culture, aseptic access C fluid medium, rotating speed 160-200r/min, 25-40 after activation
DEG C cultivate 4-5 day, to fermentation liquid thickness, color change visible mycelia raised growth；Continue aseptic access D fluid medium,
Rotating speed 160-200r/min, 25-40 DEG C of shaking table cultivates 4-5 day, to fermentation liquid thickness, color change, visible mycelia raised growth
And hypha biomass reaches 500 more than mg/100ml；
E, by brevibacterium flavum actication of culture, aseptic access C fluid medium, rotating speed 160-200r/min, 25-40 after activation
1-2 day cultivated by DEG C shaking table, color change muddy to bacterium solution；Continue aseptic access D fluid medium, rotating speed 160-200r/min,
1-2 day cultivated by 25-40 DEG C of shaking table, color change muddy to fermentation liquid, and smears counting through cell plates and reach 5 × 108/ ml with
On；
C) rice straw fermented bacterium liquid is prepared
The strain liquid equivalent of the Trichoderma viride of above-mentioned cultivation, aspergillus oryzae, penicillium oxalicum, delicious papulospora and brevibacterium flavum is mixed
Close, be rice straw fermented bacterium liquid；
(2) rice straw fermentation infusion concentrated solution is prepared
A) parts by weight of raw materials proportioning
Rice straw fermented bacterium liquid 15-20 rice straw 100 brown sugar 3-5 kg of water 200-250 (NH4)2SO4 10-15
(NH4)3PO4 10-15 KH2PO4 5-10 MgCl2 5-10 FeSO4 3-5
MnCl2 3-5 ZnSO4 3-5 NaSeO3 0.5-1；
B) preparation method
A) rice straw fermentation
Being smashed by rice straw is 1-2cm segment, pulls out, put into fermentation tank after soaking 12-24 hour with 2.8-3.2% lime water
In, add water, brown sugar, rice straw fermented bacterium liquid, the and (NH of 1/2 formula consumption by proportioning raw materials simultaneously4)2SO4 、
KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、NaSeO3, 30-40 DEG C, gas drive stirring fermentation 7-8 day, to visible fermentation tank
Mycelia raised growth；Continuously add the consumption (NH of another 1/24)2SO4 、KH2PO4、MgCl2 、FeSO4、MnCl2、ZnSO4、
NaSeO3, 30-40 DEG C, gas drive stirring fermentation 5-6 day, to fermentation liquid, visible a large amount of mycelia, straw significantly reduce and rotten soft, inspection
When surveying organic nitrogen content >=2.0g/100ml, i.e. complete rice straw fermentation；
B) infusion
After having fermented, fermentation liquid being transferred to the airtight heat container that adds, heating continues to be heated to 110-130 after getting rid of cold air
DEG C, stirring, infusion 20-24 hour, be completely dissolved to straw and mycelia, be rice straw fermentation infusion liquid；
C) concentrate
Infusion liquid 100 kilograms that rice straw is fermented be transferred to open wide add in heat container, continue slow fire heat and maintain micro-boiling,
Stirring, infusion 2-4 hour, make liquid concentration to 30-35 kilogram, be rice straw fermentation infusion concentrated solution；
2) rice straw Cordyceps militaris (L.) Link. mycelia infusion concentrated solution is prepared
A) Cordyceps militaris spawn liquid is prepared
A) E fluid medium is prepared: the 900-1100ml that peeled potatoes 80-120 g, dried silkworm chrysalis meal 8-12g added water boils to boiling and continues
Continuous slow fire maintains boiling 0.5-1 hour, adds brown sugar 14-16g, rice straw fermentation infusion liquid 80-120g, supplies after cooling
Water yield 1000ml, mixing, with 1mol/L salt acid for adjusting pH value as 6.0-6.5,100 DEG C of steam sterilizations 0.5-1 hour, prepare E liquid
Body culture medium；
B) F fluid medium is prepared: the infusion liquid 180-220g and water 900-1100ml that fermented by rice straw mixes, and passes through
Adding 1mol/L salt acid for adjusting pH value is 6.0-6.5, and 100 DEG C of steam sterilizations 0.5-1 hour prepare F fluid medium；
C) being activated by Cordyceps militaris spawn, the aseptic E fluid medium that is linked into, rotating speed 160-200r/min, 20-25 DEG C of shaking table is cultivated
4-5 day, to fermentation liquid thickness, color change visible mycelia raised growth；
D), after mycelia raised growth, continue aseptic access F fluid medium, cultivate 4-5 for rotating speed 160-200r/min, 20-25 DEG C
Day, to fermentation liquid thickness, color change, visible mycelia raised growth, hypha biomass reaches more than 500mg/100ml, is pupa
Cordyceps species liquid；
B) rice straw Cordyceps militaris (L.) Link. hypha fermentation infusion concentrated solution is prepared
100 kg of water rice straw fermentation infusion liquid are joined in fermenting tank, adds Cordyceps militaris spawn liquid 4-6 kilogram, 20-
25 DEG C of gas drive stir culture 5-7 days, to fermentation liquid thickness, occur that a large amount of mycelia and hypha biomass reach 750-850mg/100ml
Above, fermentation is terminated；After having fermented, being transferred to fermentation liquid airtight add in heat container, heating continues to add after getting rid of cold air
Hot to 100-120 DEG C, stirring, infusion 3-4 hour, it is completely dissolved to mycelia；After mycelia is completely dissolved, continue to be transferred to open wide to add
In heat container, slow fire heats and maintains micro-boiling 2-4 hour, makes liquid concentration to 30-35 kilogram, is rice straw Cordyceps militaris (L.) Link.
Hypha fermentation infusion concentrated solution；
3) rice straw fixed nitrogen bacterium solution is prepared
A) G fluid medium is prepared
By peptone 4-6g, rice straw fermentation infusion liquid 80-120g, water 900-1100ml mixing, 100 DEG C of steam sterilization 0.5-
1 hour, prepare G fluid medium；
B) H fluid medium is prepared
Rice straw is fermented infusion liquid 480-520g, water 900-1100ml mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare
H fluid medium；
C) rice straw fixed nitrogen strain liquid is prepared
By Bacillus cereus, A Shi bacillus cereus, activate addicted to sugar Pseudomonas alba, nitrogen-fixing bacteria strain, the most aseptic connect
Entering G fluid medium, 1-2 day cultivated by rotating speed 160-200r/min, 25-35 DEG C of shaking table, muddy to bacterium solution, continues aseptic access H
Fluid medium, 1-2 day cultivated by 160-200r/min, 25-35 DEG C of shaking table, muddy to each species strain bacterium solution, and puts down through cell
Plate counting reaches 5 × 108/ more than ml, is each bacterial strain strain liquid；Again by each bacterial strain strain liquid mixed in equal amounts, it is rice straw
Fixed nitrogen strain liquid；
D) rice straw fixed nitrogen bacterium solution is prepared
The infusion liquid 100 kilograms that fermented by rice straw joins in fermenting tank, and adds rice straw fixed nitrogen strain liquid 4-6
Kilogram, gas drive stirring fermentation, cultivate 1-2 day, reach 5 × 10 to cell counting for 25-40 DEG C8/ more than ml, is rice straw fixed nitrogen
Bacterium solution；
4) rice straw dissolving phosphor and dissolving potassium bacterium solution is prepared
A) I fluid medium is prepared
By peptone 4-6 g, rice straw fermentation infusion liquid 80-120g, ground phosphate rock 8-12g, feldspar in powder 4-6g, water 900-
1100ml mixes, 100 DEG C of steam sterilizations 0.5-1 hour, prepares I fluid medium；
B) J fluid medium is prepared
Rice straw is fermented infusion liquid 480-520g, ground phosphate rock 8-12g, feldspar in powder 4-6g, water 900-1100ml mixing,
100 DEG C of steam sterilizations 0.5-1 hour, prepare J fluid medium；
C) dissolving phosphor and dissolving potassium bacterial strain strain liquid is prepared
Bacillus subtillis, bacillus cereus, colloid bacillus cereus are activated, respectively aseptic access I fluid medium, turn
1-2 day cultivated by 160-200r/min, 25-35 DEG C of shaking table of speed, when each bacterial strain raised growth, bacterium solution muddiness, the most aseptic
Accessing J fluid medium, 1-2 day cultivated by rotating speed 160-200r/min, 25-35 DEG C of shaking table, cell muddy to each bacterial strain bacterium solution
Counting reaches 5 × 108/ more than ml, is each dissolving phosphor and dissolving potassium bacterial strain strain liquid；
D) rice straw dissolving phosphor and dissolving potassium bacterium solution is prepared
A) proportioning raw materials
Rice straw fermentation infusion liquid 80-120 kilogram, 1-3 kilogram of ground phosphate rock, feldspar in powder 0.5-2 kilogram；
B) preparation method
In each fermenting tank, add rice straw fermentation infusion liquid, ground phosphate rock, feldspar in powder according to formula, then add respectively
Enter each dissolving phosphor and dissolving potassium bacterial strain strain liquid 4-6 kilogram, and gas drive stirring, 25-40 DEG C of fermentation 8-10 day, reach to each strain cell counting
5×108/ more than ml, is each corresponding bacterial strain rice straw dissolving phosphor and dissolving potassium bacterium solution；By each bacterial strain bacterium solution mixed in equal amounts, it is water
Rice straw dissolving phosphor and dissolving potassium bacterium solution；
5) the pest-resistant bacterium solution of rice straw is prepared
A) pest-resistant strain liquid is prepared
A, preparation K fluid medium
By peptone 4-6 g, rice straw fermentation infusion liquid 80-120g, water 900-1100ml mixing, 100 DEG C of steam sterilizations
0.5-1 hour, prepare K fluid medium；
B, preparation L fluid medium
Rice straw fermentation infusion liquid 480-520g, water 900-1100ml mixing, 100 DEG C of steam sterilizations 0.5-1 hour, prepare L
Fluid medium；
C, being activated by bacillus thuringiensis, aseptic access K fluid medium, rotating speed 160-200r/min, 25-35 DEG C shake
1-2 day cultivated by bed, muddy to bacterium solution；Continue aseptic access L fluid medium, rotating speed 160-200r/min, 25-35 DEG C of shaking table training
Support 1-2 day, to bacterium solution is muddy and cell counting reaches 5 × 108/ more than ml, is pest-resistant strain liquid；
B) the pest-resistant bacterium solution of rice straw is prepared
In fermenting tank, add rice straw fermentation infusion liquid 100 kilograms and pest-resistant strain liquid 4-6 kilogram, 25-35 DEG C of gas
Drive stirring fermentation 1-2 day, reach 1 × 10 to bacterial strain raised growth, plate count9/ more than ml, is the pest-resistant bacterium solution of rice straw；
6) rice straw fixing nitrogen, dissolving phosphor and dissolving potassium pest-resistant organic microbial liquid fertilizer is prepared
Rice straw is fermented infusion concentrated solution, Cordyceps militaris (L.) Link. mycelia infusion concentrated solution, rice straw fixed nitrogen bacterium solution, rice straw solution
Phosphorus potassium decomposing bacterium solution, rice straw pest-resistant bacterium solution equivalent or inequality mixing, be the pest-resistant selenium-rich of rice straw fixing nitrogen, dissolving phosphor and dissolving potassium
Organic microbial liquid fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610271254.6A CN105948959A (en) | 2016-04-28 | 2016-04-28 | Rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610271254.6A CN105948959A (en) | 2016-04-28 | 2016-04-28 | Rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105948959A true CN105948959A (en) | 2016-09-21 |
Family
ID=56916808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610271254.6A Pending CN105948959A (en) | 2016-04-28 | 2016-04-28 | Rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105948959A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106565350A (en) * | 2016-11-11 | 2017-04-19 | 凤台县丰恒农业发展有限公司 | Fertilizer additive improving fertilizer efficiency of paddy field and preparation method thereof |
| CN114958683A (en) * | 2022-06-21 | 2022-08-30 | 南京林业大学 | Bacillus and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104710232A (en) * | 2013-12-16 | 2015-06-17 | 中国科学院沈阳应用生态研究所 | Organic fertilizer prepared by use of cordyceps militaris waste medium and vitamin C fermentation waste gulonic acid mother liquor and preparation method thereof |
| CN105112343A (en) * | 2015-09-28 | 2015-12-02 | 安徽飞天农用生物科技有限公司 | Wheat straw decomposition agent and method for preparing same |
-
2016
- 2016-04-28 CN CN201610271254.6A patent/CN105948959A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104710232A (en) * | 2013-12-16 | 2015-06-17 | 中国科学院沈阳应用生态研究所 | Organic fertilizer prepared by use of cordyceps militaris waste medium and vitamin C fermentation waste gulonic acid mother liquor and preparation method thereof |
| CN105112343A (en) * | 2015-09-28 | 2015-12-02 | 安徽飞天农用生物科技有限公司 | Wheat straw decomposition agent and method for preparing same |
Non-Patent Citations (1)
| Title |
|---|
| 上海市经济和信息化委员会等: "《2010世界制造业重点行业发展动态》", 30 June 2011, 上海科学技术文献出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106565350A (en) * | 2016-11-11 | 2017-04-19 | 凤台县丰恒农业发展有限公司 | Fertilizer additive improving fertilizer efficiency of paddy field and preparation method thereof |
| CN114958683A (en) * | 2022-06-21 | 2022-08-30 | 南京林业大学 | Bacillus and application thereof |
| CN114958683B (en) * | 2022-06-21 | 2023-05-05 | 南京林业大学 | Bacillus and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103819267B (en) | For the microbial-bacterial fertilizer and preparation method thereof of peanut cultivation | |
| CN104560828B (en) | Microbial agent and application in prospect of passivating heavy metals of soil | |
| CN104609979B (en) | A kind of microbial-bacterial fertilizer for improveing salt affected soil and preparation method thereof | |
| CN109369269B (en) | Biochar compound microbial fertilizer for inhibiting soil-borne diseases and preparation method thereof | |
| CN106434430A (en) | Compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof | |
| CN104560045B (en) | Microbial bacterial agent and chemical fertilizer excessively use growth-promoting secondary saline and alkaline and prone soil improved application | |
| CN106747933A (en) | A kind of Biological organic fertilizer special for cucumber and preparation method thereof | |
| CN102775202A (en) | Novel microbial organic fertilizer with anti-actinomyces active compound bacteria and preparation method thereof | |
| CN102199049A (en) | Microbial organic manure and preparation method thereof | |
| CN103772011B (en) | The liquid bacterial manure of a kind of cereal special composite biological | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| CN104311334A (en) | Composite improver for repairing heavy metal contaminated soil and preparation process | |
| CN102276351B (en) | Multifunctional total-nutrient fertilizer and its production method | |
| CN105948888A (en) | Multifunctional efficient ecological organic biological liquid fertilizer | |
| CN107382496A (en) | A kind of artificial soil for strengthening crop anti-adversity and the method for strengthening crop anti-adversity | |
| CN103141519B (en) | Preparation method and application of anti-biological inoculants for controlling crop soil-borne diseases | |
| KR101844633B1 (en) | The fermentation liquid of complex functional materials and manufacturing method thereof | |
| CN106591376A (en) | Microbial culture product for preventing and controlling anthomyiidae larvae and underground nematodes, and preparation method thereof, and prevention and control method | |
| CN105967770B (en) | A kind of organic Chinese medicine liquid fertilizer of the pest-resistant diseases prevention of rice straw | |
| CN105272611A (en) | Formula and production method of culture substrate for horticultural crops | |
| CN105175159A (en) | Production method of rice seedling dry raising and strengthening biological medium and application thereof | |
| CN102503695A (en) | Microorganism bacterial fertilizer and preparation method thereof | |
| CN104496575A (en) | Organic fertilizer for resisting continuous cropping obstacles of chuzhou chrysanthemum and preparation method of organic fertilizer | |
| CN105948959A (en) | Rice-straw nitrogen-fixation phosphorus-dissolving potassium-dissolving insect-resistant selenium-enriched organic microbial liquid fertilizer | |
| CN106396861A (en) | Compound microbial bamboo shoot fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160921 |
|
| RJ01 | Rejection of invention patent application after publication |