CN105948842B - Livestock and poultry dung recovery produces lichens micro-organisms bacillus fertilizer - Google Patents
Livestock and poultry dung recovery produces lichens micro-organisms bacillus fertilizer Download PDFInfo
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- CN105948842B CN105948842B CN201610284586.8A CN201610284586A CN105948842B CN 105948842 B CN105948842 B CN 105948842B CN 201610284586 A CN201610284586 A CN 201610284586A CN 105948842 B CN105948842 B CN 105948842B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention relates to a kind of resource utilization feces of livestock and poultry production controlling plant diseases, the Low-cost production method for the bacillus licheniformis microbial manure for promoting plant growth, belong to poultry excrement and urine disposal and microbial manure technical field.The bacterial strain that the present invention uses is plant height effect plant growth-promoting bacteria --- bacillus licheniformis (Bacillus licheniformis) B4 bacterial strain that separation screening comes out from soil, the bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC No.6677.The present invention is using feces of livestock and poultry and starch wastewater as fermentation medium, large-scale culture bacillus licheniformis, prepare the microbial manure that there are controlling plant diseases, promote plant growth, the technique low production cost, it is easy to operate, feces of livestock and poultry can also be made to get utilization, reduce disposal of pollutants.Microbial inoculum produced by the invention is used for the production of tealeaves and vegetables, yield not only can be improved, but also can improve the quality of tea place and vegetables, shows wide application prospect.
Description
Technical field
The invention belongs to poultry excrement and urine disposals and microbial manure technical field.
Technical background
In recent years, the intensive livestock and poultry breeding industry development in China is swift and violent, obtains huge Social benefit and economic benefit, together
When also produce a large amount of animal faeces wastes.The data in national environmental protection portion show that the annual feces of livestock and poultry in China discharges
Total amount is more than 2,500,000,000 tons, has become the main source of Rural Plane Source Pollution.Since most of farms do not have prevention and cure of pollution to set
It applies or environmental protection facility is not perfect, a large amount of excrement, sewage are discharged into environment without being effectively treated, and polluted source and air propagate disease
A series of harm such as bacterium, polluted agricultural land bring serious influence to agricultural production and ecological environment, it is therefore necessary to carry out harmless
Change processing.In addition, containing the good nutrition substrate that a large amount of organic matters are microorganism growths in feces of livestock and poultry, microorganism can be made
Culture medium, fermenting and producing microniological proudcts.Therefore, exploiting natural resources produces microbial manure technology using feces of livestock and poultry, not only
It can turn waste into wealth, reduce pollution sources, and the application of microbial manure can also reduce chemical fertilizer and pesticide in agricultural production
Usage amount, it is energy saving, improve the quality of agricultural product, the requirement of Complex Development circular economy and green agriculture.
The processing method of feces of livestock and poultry reported in the literature mainly has compost, fermentation methane, directly agricultural etc..Chinese patent
(application number: a kind of swine manure wastewater processing qualified discharge technology is disclosed in 200410060847.5), can effectively be controlled
It pollutes environment.(application number: disclosing a kind of livestock excrement composting processing technique to Chinese patent in 200410060846.0), can
To produce good organic fertilizer, for improveing soil.Chinese patent (application number: discloses one kind in 200610089709.9)
Animal dung and liquid dung fast harmless biological treating process, using waxy Bacillus, colloid bacillus cereus, trichoderma, condensation bud
The complex micro organism fungicide that spore bacillus, saccharomycete, bacillus subtilis form, is inoculated in excrement, adds filler material, fermented
After form organic fertilizer.Chinese patent (application number: discloses a kind of feces of livestock and poultry harmless treatment side in 200810153855.2)
Method is that farmyard manure is added and zymophyte powder, compost fermentation form after feces of livestock and poultry is mixed with stalk, straw, sawdust.China
Patent (application number: discloses a kind of microorganism for producing biological organic fertilizer by livestock and poultry manure fermentation in 201010247234.4)
The preparation method of decomposing agent includes: brewer's yeast, lactobacillus acidophilus, Rhodopseudomonas palustris in the decomposing agent.Chinese patent
A kind of harmless treatment method for livestock manure, including animal wastes fermentation, fly are disclosed in (application number: 201010594669.X)
Maggot cultivation, biogas preparation and microorganism cultivate four steps.
Bacillus licheniformis (Bacillus licheniformis) is widely distributed in nature, is a kind of important life
Fungi-proofing, it inhibits plant pathogenetic bacteria, fungi, virus, line by generating antibiotic, antibacterial protein or polypeptide isoreactivity substance
Worm, to play the role of biological control；It is also a kind of important plant rhizosphere growth-promoting bacterium simultaneously, is shown to a variety of plants
Object promotes the effect of growth.For example, Chinese invention patent (application number: discloses one plant of lichens bud in 200310115535.5)
Spore bacillus B-0A12 bacterial strain, deposit number is CGMCC No.1035, for preventing and treating caused by soybean cyst nematode and sickle-like bacteria
Root rot, culture medium used in the strain fermentation incubation are as follows: soluble starch 1-2%, beancake powder 1-2%, beef extract
0.5-1.0%, glucose 0.1-0.5%, there are also a small amount of inorganic salts, bacterial content is 7 × 10 when fermentation ends8A/milliliter.In
State's patent of invention (application number: discloses a plants endogenetic bacillus licheniformis R21 bacterial strain, preservation in 200810026137.9)
Number is CCTCC M 207113, has wide spectrum antagonistic plant pathogenic fungi ability, dissolving P capacity and promotes plant growth ability,
It, which ferments, uses LB culture medium, and discloses it and prevent and treat celery verticillium wilt, banana blight, and promotes celery growth aspect
Application.(application number: disclosing 201 bacterial strain of a bacillus licheniformis to Chinese invention patent in 200810183072.9), protects
Hiding number is CCTCC No.M207092, the bacterial strain to banana blight, watermelon blight, tomato wilt, banana anthracnose,
A variety of disease fungus such as wheat scab have antagonism, and the culture of the bacterial strain uses beef-protein medium.China
(application number: disclosing 202 bacterial strain of a bacillus licheniformis to patent of invention in 200810183075.2), deposit number is
CCTCC No.M207093, it also has the function of the biological and ecological methods to prevent plant disease, pests, and erosion similar with 201 bacterial strains.
The application of bacillus licheniformis industrially is also than wide.For example, Chinese invention patent (application number:
200810227858.6) a bacillus licheniformis U1-3 bacterial strain is disclosed in, deposit number is CGMCC No.2437, the bacterium
Strain in petroleum production engineering field can be used to the displacement of reservoir oil, improve oil recovery, culture medium use atoleine, yeast powder and
Plurality of inorganic salt.Chinese invention patent (application number: discloses a bacillus licheniformis WX-02 bacterium in 200810055068.4)
Strain, deposit number are CCTCC No.M208065, and for producing Polyurethane-epoxy resin, culture medium uses sodium glutamate, carbon source
(one of starch or starch hydrolyzate, glucose, fructose, maltose, sucrose), inorganic nitrogen-sourced and other plurality of inorganic salt.
Chinese invention patent (application number: discloses a bacillus licheniformis HM-3 bacterial strain, deposit number in 200810026812.8)
For CGMCC No.1970, for producing alkali protease, culture medium uses yeast extract, peptone and municipal sludge.Middle promulgated by the State Council
(application number: disclosing a bacillus licheniformis B-05571 bacterial strain to bright patent in 200610053548.8), deposit number is
CCTCC No.M206082, for converting glycerol into dihydroxyacetone (DHA), culture medium is using glycerol as primary carbon source, and there are also yeast
Powder, peptone, beef extract, plurality of inorganic salt.
Have no that resource utilization feces of livestock and poultry production bacillus licheniformis (Bacillus licheniformis) is micro- at present
The report of bio-feritlizer.Production cost can be not only reduced come grown place clothing micro-organisms bacillus fertilizer using feces of livestock and poultry,
And it can be with the such organic waste of recycling treatment.
According to the definition in the agricultural industry criteria " microbial manure term " (NY/T 1113-2006) of microbial manure,
Microbial manure is the product containing specified microorganisms living body, is applied to agricultural production, passes through the life of microorganism contained therein
Activity increases the supply of plant nutrient or promotes plant growth, improves yield, improve quality of agricultural product and Agro-ecology ring
Border.Currently, microbial manure includes microbial inoculant, composite microbiological fertilizer and biological organic fertilizer.
Summary of the invention
The object of the present invention is to provide a kind of harmless treatment feces of livestock and poultry and for producing lichens micro-organisms bacillus
The method of fertilizer.
Bacterial strain used in the present invention is that the plant height effect that separation screening comes out from soil by inventor promotes plant raw
Long, antagonistic phytopathogen bacillus licheniformis (Bacillus licheniformis) B4 bacterial strain, the bacterial strain was in 2012
October 15 was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC
No.6677, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
The method of feces of livestock and poultry harmless treatment production lichens micro-organisms bacillus fertilizer of the present invention is as follows:
1, prepared by fluid nutrient medium: adding the starch wastewater such as starch production process discharge into feces of livestock and poultry, is adjusted to solid
The weight percent of shape object content be 4%~8%, be beaten with refiner, 115 DEG C high pressure sterilization 15~30 minutes.
2, prepared by liquid spawn: the lichens of slant preservation is inoculated with into the triangular flask equipped with the fluid nutrient medium pre-processed
Bacillus, in 30~37 DEG C, 120~200 revs/min shaken cultivation 20~36 hours, obtain liquid seeds.
3, liquid spawn expands culture: into the fermentor equipped with the fluid nutrient medium pre-processed, inoculation volume ratio is
3%~10% liquid spawn is led to filtrated air culture 18~36 hours in 30~37 DEG C；Cultured fermentation liquid is through plate
Count of bacteria, viable count reach 2 × 109CFU/ milliliters or more, it can be used as liquid microbe Fertilizer application.
4, solid state fermentation: using the stalk of crushing, sawdust or rice husk as conditioner, with feces of livestock and poultry according to weight ratio 1:5~
3:1 mixing, makes the water content 50%~55% of material after mixing, is according to weight ratio by cultured liquid microbe fertilizer
5%~10% inoculum concentration is added in mixed material, stirs evenly, and static culture 10~15 days, forces in incubation logical
Wind or turning in 1~3 day 1 time, hot stage control temperature of charge between 56~68 DEG C, maintain 5 days or more, it is ensured that kill livestock and poultry
Pathogen and parasitic ovum in excrement；Obtain microbial manure primary product.
5, the preparation of solid microbe fertilizer: the liquid of third step acquisition is added in right amount into microbial manure primary product
Body microbial manure makes the viable count of bacillus licheniformis in product reach 5 × 108CFU/ grams or more, aeration-drying is to aqueous
25% or less amount；Packaging is stored at shady and cool drying.
Specific embodiment:
The microbial manure of technical solution production provided by the invention, which is applied to agricultural production, can reduce chemical fertilizer and pesticide
Usage amount, so as to improve farm environment quality；And production cost is very low, and high-concentration waste water is made to get utilization, and subtracts
The discharge of of low pollution object.Specific embodiment is as follows:
Embodiment 1: livestock and poultry dung recovery process for producing bacillus licheniformis microbial manure
1, prepared by fluid nutrient medium: adding the starch wastewater of Starch Production enterprise discharge into feces of livestock and poultry, is adjusted to solid
The weight percent of object content be 6%, be beaten with refiner, 115 DEG C high pressure sterilization 20 minutes.
2, prepared by liquid spawn: the lichens of slant preservation is inoculated with into the triangular flask equipped with the fluid nutrient medium pre-processed
Bacillus, in 33 DEG C, 150 revs/min shaken cultivation 26 hours, obtain liquid seeds.
3, liquid spawn expands culture: into the fermentor equipped with the fluid nutrient medium pre-processed, inoculation volume ratio is
5% liquid spawn is led to filtrated air culture 20 hours in 33 DEG C；Cultured fermentation liquid is through count plate, viable count
Reach 2 × 109CFU/ milliliters or more, it can be used as liquid microbe Fertilizer application.
4, solid state fermentation: mixed according to weight ratio 1:1 with feces of livestock and poultry using the stalk of crushing, sawdust or rice husk as conditioner
It closes, makes the water content 50%~55% of material after mixing, according to weight ratio be 8% by cultured liquid microbe fertilizer
Inoculum concentration is added in mixed material, stirs evenly, and static culture 12 days, forced ventilation or turning 1 in 1~3 day in incubation
Secondary, hot stage controls temperature of charge between 56~68 DEG C, maintain 5 days or more, it is ensured that kill the pathogen in feces of livestock and poultry
And parasitic ovum；Obtain microbial manure primary product.
5, the preparation of solid microbe fertilizer: the liquid of third step acquisition is added in right amount into microbial manure primary product
Body microbial manure makes the viable count of bacillus licheniformis in product reach 5 × 108CFU/ grams or more, aeration-drying is to aqueous
25% or less amount；Packaging is stored at shady and cool drying.
Embodiment 2: bacillus licheniformis microbial manure is applied to Tea Production
One piece of tea tree growing way is selected uniformly experimental field in tea place, is evenly dividing as 6 cells, and each cell about 200 is flat
Square rice, 3 kinds of control (CK) that is respectively set to not apply fertilizer, application of organic fertilizers (OF) and application microbial manure (MF) processing, every kind
Processing 2 parallel, 2 meters or so of isolation strip is designed between each processing, organic fertilizer and microbial manure are according to 500 kilograms per acre
Dosage, ditch spread 1 time before entering the winter, spring harvests tealeaves, the results showed that the spring tea yield increased of application of organic fertilizers is increased production
17% or so, and the spring tea yield increased volume increase 35% or so of microbial manure is applied, and apply the tea of microbial manure
The content of water extraction, tea polyphenols and amino acid is significantly higher than control in leaf.
Claims (1)
1. a kind of livestock and poultry dung recovery utilizes production bacillus licheniformis (Bacillus licheniformis) microorganism fertilizer
The method of material, which is characterized in that this method comprises the following steps:
(1) prepared by fluid nutrient medium: the starch wastewater of starch production process discharge being added into feces of livestock and poultry, is adjusted to solid content
The weight percent of content be 4%~8%, be beaten with refiner, 115 DEG C high pressure sterilization 15~30 minutes；
(2) prepared by liquid spawn: the lichens bud of slant preservation is inoculated with into the triangular flask equipped with the fluid nutrient medium pre-processed
Spore bacillus, in 30~37 DEG C, 120~200 revs/min shaken cultivation 20~36 hours, obtain liquid seeds；
(3) liquid spawn expands culture: be inoculated in equipped with the fermentor of fluid nutrient medium pre-process volume ratio for 3%~
10% liquid spawn is led to filtrated air culture 18~36 hours in 30~37 DEG C；Cultured fermentation liquid is through plate bacterium meter
Number, viable count reach 2 × 109CFU/ milliliters or more, as liquid microbe Fertilizer application；
(4) solid state fermentation: using the stalk of crushing, sawdust or rice husk as conditioner, with feces of livestock and poultry according to weight ratio 1:5~3:1
Mixing, make mixing after material water content 50%~55%, by cultured liquid microbe fertilizer according to weight ratio be 5%
~10% inoculum concentration is added in mixed material, stirs evenly, static culture 10~15 days, in incubation forced ventilation or
Turning in 1~3 day 1 time, hot stage control temperature of charge between 56~68 DEG C, maintain 5 days or more, it is ensured that kill feces of livestock and poultry
In pathogen and parasitic ovum；Obtain microbial manure primary product；
(5) preparation of solid microbe fertilizer: the liquid for adding third step acquisition in right amount into microbial manure primary product is micro-
Bio-feritlizer makes the viable count of bacillus licheniformis in product reach 5 × 108CFU/ grams or more, aeration-drying to water content
25% or less；Packaging is stored at shady and cool drying.
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| CN201610284586.8A CN105948842B (en) | 2016-05-03 | 2016-05-03 | Livestock and poultry dung recovery produces lichens micro-organisms bacillus fertilizer |
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| CN108558484A (en) * | 2017-10-24 | 2018-09-21 | 河北丰禾绿光农业科技有限公司 | A kind of selenium-enriched microbe fertilizer and preparation method thereof |
| CN109020644A (en) * | 2018-09-05 | 2018-12-18 | 河北大学 | Utilize the method and application of Pig raising wastewater fermenting and producing microbial manure |
| CN109456091A (en) * | 2018-09-05 | 2019-03-12 | 河北大学 | Utilize the method and application of cowboying wastewater fermentation production microbial manure |
| CN114940635A (en) * | 2022-06-08 | 2022-08-26 | 中资蓝天生态环境科技有限公司 | Method for reducing emission of greenhouse gas by organic solid waste semipermeable membrane fermentation and preparing microbial fertilizer |
| CN117736036A (en) * | 2024-02-04 | 2024-03-22 | 广东中施龙泰低碳科技有限公司 | Method for preparing bio-organic fertilizer by recycling earthworm feces |
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| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
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