CN103320369A - Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof - Google Patents
Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof Download PDFInfo
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- CN103320369A CN103320369A CN2013102922729A CN201310292272A CN103320369A CN 103320369 A CN103320369 A CN 103320369A CN 2013102922729 A CN2013102922729 A CN 2013102922729A CN 201310292272 A CN201310292272 A CN 201310292272A CN 103320369 A CN103320369 A CN 103320369A
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- 239000002131 composite material Substances 0.000 title claims abstract description 32
- 239000010815 organic waste Substances 0.000 title claims abstract description 21
- 229920002678 cellulose Polymers 0.000 title claims abstract description 19
- 239000001913 cellulose Substances 0.000 title claims abstract description 18
- 239000002054 inoculum Substances 0.000 title claims abstract description 8
- 230000001737 promoting effect Effects 0.000 title claims abstract 5
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 34
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 33
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 12
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims abstract description 12
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 12
- 241000187758 Streptomyces ambofaciens Species 0.000 claims abstract description 12
- 241001313536 Thermothelomyces thermophila Species 0.000 claims abstract description 12
- 241000223259 Trichoderma Species 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims description 73
- 241000233866 Fungi Species 0.000 claims description 62
- 239000002068 microbial inoculum Substances 0.000 claims description 40
- 239000000463 material Substances 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 29
- 239000012531 culture fluid Substances 0.000 claims description 28
- 238000000855 fermentation Methods 0.000 claims description 24
- 230000004151 fermentation Effects 0.000 claims description 24
- 241001655322 Streptomycetales Species 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 21
- 239000002023 wood Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 19
- 239000007787 solid Substances 0.000 claims description 18
- 238000009264 composting Methods 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000012258 culturing Methods 0.000 claims description 16
- 240000003183 Manihot esculenta Species 0.000 claims description 15
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 13
- 238000000354 decomposition reaction Methods 0.000 claims description 10
- 230000003203 everyday effect Effects 0.000 claims description 9
- 239000004021 humic acid Substances 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 239000002361 compost Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000003895 organic fertilizer Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 description 17
- 230000001580 bacterial effect Effects 0.000 description 14
- 239000002609 medium Substances 0.000 description 14
- 235000010980 cellulose Nutrition 0.000 description 12
- 239000002699 waste material Substances 0.000 description 8
- 229920001817 Agar Polymers 0.000 description 7
- 239000008272 agar Substances 0.000 description 7
- 238000001035 drying Methods 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 4
- 239000004744 fabric Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000002386 leaching Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229940022682 acetone Drugs 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011162 core material Substances 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
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- 235000010987 pectin Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a composite inoculant for promoting the decomposing of cellulose organic wastes, and a preparation method thereof. The active components of the inoculant for promoting the decomposing of cellulose organic wastes comprise Sporotrichum thermophile, Trichoderma konigii, Phanerochaete chrysosporium, Aspergillus niger, Saccharomyces cerevisiae, Bacillus subtilis and Streptomyces ambofaciens. Experiments in the invention prove that the composite inoculant for promoting the decomposing of cellulose organic wastes can effectively improve the decomposing speed of the organic wastes, and can be used for producing biological organic fertilizers.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of composite fungus agent and preparation method who promotes that organic waste becomes thoroughly decomposed.
Background technology
The organic wastes such as the crop material of the annual generation of the light industry such as agriculture production and food, extraction using alcohol enormous amount, acid-sludge, schlempe, the difficult decomposed substances such as the most rich cellulose of this class waste, xylogen, cause these organic waste utilization ratios not high, great majority are all processed as " rubbish ", not only contaminate environment but also cause the wasting of resources.The microbial inoculum that is commonly used at present to process organic waste both at home and abroad mostly is greatly single microbial inoculum and the spontaneous fermentation thing microbial inoculum of purifying.The bacterium of the single microbial inoculum of purifying is narrow to the subject range of growth conditions, and is high to the fermentation condition requirement, and easily is subject to pollution and the inhibition of other bacterium; Owing to having lost the coexistence that is in for a long time other bacterium of conspiracy relation under the natural condition, the capacity of decomposition of its organic waste reduces greatly.Contained bacterial classification is many bacterium association that disorderly set forms in the passive fermenting process of nature in the spontaneous fermentation thing microbial inoculum, can't grasp its bacterial classification and form, and lacks science, and result of use is difficult to control; The fermenting process of spontaneous fermentation thing microbial inoculum is followed the circulation law of nature, and its decomposition efficiency is low, and effect is unstable.
Summary of the invention
An object of the present invention is to provide a kind of microbial inoculum that promotes that composting material becomes thoroughly decomposed.
The microbial inoculum that promotion composting material provided by the invention becomes thoroughly decomposed, its activeconstituents are thermophilic fungus destroyed wire (Sporotrichum thermophile), healthy and free from worry wood mould (Trichoderma konigii), whiterot fungi (Phanerochaete chrysosporium), aspergillus niger (Aspergillus niger), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), subtilis (Bacillus subtilis) CICC and produce dyadic streptomycete (Streptomyces ambofaciens) ACCC.
Specifically can be: thermophilic fungus destroyed wire (Sporotrichum thermophile) CICC2441, healthy and free from worry wood mould (Trichoderma konigii) CGMCC3.04001, whiterot fungi (Phanerochaete chrysosporium) CGMCC5.00776, aspergillus niger (Aspergillus niger) CGMCC3.3147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC1415, subtilis (Bacillus subtilis) CICC10076 and product dyadic streptomycete (Streptomyces ambofaciens) ACCC40133.
In the above-mentioned microbial inoculum, the living bacteria count of described microbial inoculum is greater than 0.5 * 10
8Requirement in the standard " agricultural microorganism microbial inoculum national standard (GB20287-2006) " of cfu/g(agricultural microbial preparation, the living bacteria count of organic material composting solid fungicide needs greater than 0.5 * 10
8Cfu/g).In the microbial inoculum, described thermophilic fungus destroyed wire (being specially thermophilic fungus destroyed wire CICC2441), described healthy and free from worry wood mould (being specially the mould CGMCC3.04001 of healthy and free from worry wood), described whiterot fungi (being specially whiterot fungi CGMCC5.00776), described aspergillus niger (being specially aspergillus niger CGMCC3.3147), described yeast saccharomyces cerevisiae (being specially yeast saccharomyces cerevisiae CICC1415), the ratio of the living bacteria count of described subtilis (being specially subtilis CICC10076) and described product dyadic streptomycete (be specially and produce dyadic streptomycete ACCC40133) is 1:(1-30): (0.1-5): (1-50): (0.1-5): (1-50): (1-30).
The quality percentage composition of moisture is 12-16% in the above-mentioned microbial inoculum; The living bacteria count of described microbial inoculum is 2.8 * 10
8Cfu/g-5.2 * 10
8Cfu/g; The living bacteria count of described microbial inoculum is specially 2.8 * 10
8Cfu/g, 3.2 * 10
8Cfu/g, 4.7 * 10
8Cfu/g or 5.2 * 10
8Cfu/g.
Above-mentioned microbial inoculum packing, store method are: adopt opaque plastics bag to pack, every bag of 0.5-2Kg places the environment of drying, cool place, ventilation to preserve, preservation period 6 months.
Another object of the present invention provides a kind of method for preparing above-mentioned microbial inoculum.
Method provided by the invention comprises the steps:
1) following 7 kinds of bacteria culture fluids is mixed, obtain composite bacteria liquid: thermophilic fungus destroyed wire (Sporotrichum thermophile, be specially thermophilic fungus destroyed wire CICC2441), mould (the Trichoderma konigii of healthy and free from worry wood, be specially the mould CGMCC3.04001 of healthy and free from worry wood), whiterot fungi (Phanerochaete chrysosporium, be specially whiterot fungi CGMCC5.00776), aspergillus niger (Aspergillus niger, be specially aspergillus niger CGMCC3.3147), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae, be specially yeast saccharomyces cerevisiae CICC1415), subtilis (Bacillus subtilis, be specially subtilis CICC10076) and produce dyadic streptomycete (Streptomyces ambofaciens is specially and produces dyadic streptomycete ACCC40133);
Described bacteria culture fluid is for cultivating described bacterium, the bacteria culture fluid that obtains;
2) mix adding water behind the described composite bacteria liquid access solid enlarged culturing base, obtain culture base-material, the described culture base-material of fermentation culture is collected tunning, namely obtains microbial inoculum.
In the aforesaid method, in the step 1), the living bacteria count of described 7 kinds of bacteria culture fluids is all greater than 1 * 10
7Cfu/ml;
The mass ratio of described 7 kinds of bacteria culture fluids is followed successively by: 1:(0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1); The mass mixing such as specifically can be;
Step 2) in, described composite bacteria liquid is to add water behind the inoculum size access solid enlarged culturing base of 4%-5% to be mixed to the biodiversity percentage amounts be 55%-60% by the quality volume percent.
In the aforesaid method, the living bacteria count of described 7 kinds of bacteria culture fluids respectively is 1.95-4.6 * 10
7Cfu/ml, 0.87-5.83 * 10
8Cfu/ml, 1.26-7.63 * 10
7Cfu/ml, 1.4-9.47 * 10
8Cfu/ml, 0.87-9.57 * 10
7Cfu/ml, 1.52-8.8 * 10
8Cfu/ml and 1.39-4.67 * 10
8Cfu/ml;
The culture condition that obtains of described 7 kinds of bacteria culture fluids is: cultivate 72-84h at 28-30 ℃, 200rpm;
Step 2) in, described solid enlarged culturing base is prepared as follows: with the cassava starch dregs of particle diameter 0.425-0.85mm, add in mass ratio 10% Semen Maydis powder, mixing obtains solid enlarged culturing base;
The condition of described fermentation culture is specific as follows: 28-35 ℃, described culture base-material stacking height be stir 10-14cm, every day 1-2 time, through 5-8 days fermentation culture, obtain tunning.
Be specially:
28 ℃, described culture base-material stacking height be stir 10cm, every day 1 time, through 8 days fermentation culture, obtain tunning;
Or 35 ℃, described culture base-material stacking height be stir 14cm, every day 2 times, through 5 days fermentation culture, obtain tunning;
In step 2) the collection tunning after, comprise the steps: that also it is 12-16% that tunning is dried to the biodiversity percentage amounts, obtains microbial inoculum.
Above-mentioned drying can be dried or oven dry below 50 ℃, adopts in an embodiment 45 ℃ of oven dry.
Above-mentioned thermophilic fungus destroyed wire (Sporotrichum thermophile, be specially thermophilic fungus destroyed wire CICC2441) bacteria culture fluid, mould (the Trichoderma konigii of healthy and free from worry wood, be specially the mould CGMCC3.04001 of healthy and free from worry wood) bacteria culture fluid, whiterot fungi (Phanerochaete chrysosporium, be specially whiterot fungi CGMCC5.00776) bacteria culture fluid, aspergillus niger (Aspergillus niger, be specially aspergillus niger CGMCC3.3147) bacteria culture fluid, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae, be specially yeast saccharomyces cerevisiae CICC1415) bacteria culture fluid, subtilis (Bacillus subtilis, be specially subtilis CICC10076) bacteria culture fluid and product dyadic streptomycete (Streptomyces ambofaciens, being specially and producing dyadic streptomycete ACCC40133) bacteria culture fluid all can prepare according to ordinary method, specifically can be the method for following steps:
A, slant strains cultivate: every kind of bacterium is inoculated on the corresponding slant medium, is to leave standstill under 26-30 ℃ the condition to cultivate 24-48 hour in temperature; Be specially under temperature is 26 ℃ condition, to leave standstill to leave standstill under the condition of cultivating 48 hours or 30 ℃ and cultivated 24 hours;
B, liquid seeds enlarged culturing: the inoculation behind the above-mentioned slant culture of picking is in corresponding liquid nutrient medium, and the 200rpm shaking table was cultivated 72-96 hour under 26-30 ℃ condition, obtains corresponding bacteria culture fluid; Be specially at 28 ℃, 200rpm and cultivate 72h or cultivate 84h at 30 ℃, 200rpm;
Above-mentioned thermophilic fungus destroyed wire (Sporotrichum thermophile, be specially thermophilic fungus destroyed wire CICC2441) slant medium be prepared as follows: potato leaching liquid 1L, glucose 10.0g, agar 15.0g mix, and the pH value is the nature value;
Mould (the Trichoderma konigii of healthy and free from worry wood, be specially the mould CGMCC3.04001 of healthy and free from worry wood) and whiterot fungi (Phanerochaete chrysosporium, be specially whiterot fungi CGMCC5.00776) slant medium all be prepared as follows: potato leaching liquid 1L, glucose 20.0g, agar 15.0g mix, and the pH value is the nature value.
The slant medium of aspergillus niger (Aspergillus niger is specially aspergillus niger CGMCC3.3147) is prepared as follows: sucrose 30.0g, NaNO
33.0g, MgSO
47H
2O0.5g, KCl0.5g, FeSO
44H
2O0.01g, K
2HPO
41.0g, agar 15.0g, distilled water 1.0L mix pH6.0-6.5;
The slant medium of subtilis (Bacillus subtilis is specially subtilis CICC10076) is prepared as follows: extractum carnis 10.0g, peptone 10.0g, pectin 10.0g, glucose 5.0g, NaCl3.0g, K
2HPO
41.0g, distilled water 1.0L, agar 20.0g mix pH7.5;
The slant medium of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae is specially yeast saccharomyces cerevisiae CICC1415) is prepared as follows: brown sugar 50.0g, urea 1.0g, agar 20.0g, distilled water 1L mix pH5.0-5.6;
The slant medium that produces dyadic streptomycete (Streptomyces ambofaciens is specially and produces dyadic streptomycete ACCC40133) is prepared as follows: Zulkovsky starch 20.0g, KNO
31.0g, MgSO
47H
2O0.5g, NaCl0.5g, FeSO
44H
2O0.01g, K
2HPO
40.5g, agar 15.0g, distilled water 1.0L mix pH7.2-7.4.
Above-mentioned potato leaching liquid is prepared as follows: remove skin potato 200g, be cut into small pieces, add water 1.0L and boil 30min, the elimination potato ball complements to 1.0L with filtrate.
The used liquid nutrient medium of above-mentioned each bacterial strain gets final product for the slant culture based formulas of each bacterial strain deducts agar.
Above-mentioned microbial inoculum or the application of microbial inoculum in production biological organic fertilizer, compost or promotion composting material become thoroughly decomposed of adopting aforesaid method to prepare also are the scope of protection of the invention.
In the above-mentioned application, described composting material is organic waste; Described organic waste is the cellulose family organic waste; Described cellulose family organic waste is that 75% organic cassava alcohol slag and quality percentage composition are that 25% filter mud forms by the quality percentage composition.
Described composting material is 450-550:1 with the functional quality ratio of described microbial inoculum.
In the above-mentioned application, described promotion composting material becomes thoroughly decomposed to be embodied in and promotes cellulose decomposition (being embodied in the cellulose decomposition rate that improves) and/or improve humic acids content.
Of the present invention experiment showed, the invention provides a kind of composite fungus agent that promotes that the cellulose family organic waste becomes thoroughly decomposed, and it is the combination of multiple bacterium, is comprised of the various bacteria, fungi, the actinomycetes that have material decomposition functions such as Mierocrystalline celluloses; This microbial inoculum viable count is high, act synergistically, act on stable, and the speed of becoming thoroughly decomposed that can the Effective Raise organic waste can be used for producing biological organic fertilizer, especially can significantly promote the decomposition of Mierocrystalline cellulose etc., accelerates course of fermentation; Reduced simultaneously the production cost of complex micro organism fungicide, improved with composite microbiological fertilizer and the biological organic fertilizer competitiveness of product in market of this microbial inoculum as core material.
Embodiment
Employed experimental technique is ordinary method if no special instructions among the following embodiment.
Used bacterial strain, material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Bacteria used thereby is thermophilic fungus destroyed wire (Myceliophthora thermophila) preserving number CICC2441 among the following embodiment, healthy and free from worry wood mould (Trichoderma konigii) preserving number CGMCC3.04001, whiterot fungi (Phanerochaete chrysosporium) preserving number CGMCC5.00776, aspergillus niger (Aspergillus niger) preserving number CGMCC3.03147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) preserving number CICC1415, subtilis (Bacillus subtilis) preserving number CICC10076, produce dyadic streptomycete (Streptomyces ambofaciens) preserving number ACCC40133.
The cultivation of above-mentioned 7 kinds of bacterial strains all can be carried out according to ordinary method, the concrete visible summary of the invention part of its solid slant culture base and liquid nutrient medium.
Solid enlarged culturing base is prepared as follows: with the cassava starch dregs of particle diameter 0.425-0.85mm, add Semen Maydis powder in the ratio of this cassava starch dregs quality 10%, stir, obtain solid enlarged culturing base.
The preparation of embodiment 1, composite fungus agent and detection
1, slant strains is cultivated
Thermophilic fungus destroyed wire (Sporotrichum thermophile) preserving number CICC2441, healthy and free from worry wood mould (Trichoderma konigii) preserving number CGMCC3.4001, whiterot fungi (Phanerochaete chrysosporium) preserving number CGMCC5.776, aspergillus niger (Aspergillus niger) preserving number CGMCC3.03147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) preserving number CICC1415, subtilis (Bacillus subtilis) preserving number CICC10076, producing these 7 kinds of bacterial strains of dyadic streptomycete (Streptomyces ambofaciens) preserving number ACCC40133 and be inoculated in respectively on the above-mentioned corresponding slant medium, is to leave standstill under 26 ℃ the condition to cultivate 48 hours in temperature.
2, liquid seeds enlarged culturing
Each bacterial strain behind the picking slant culture is inoculated in respectively in the corresponding liquid nutrient medium (1L culture medium inoculated 4-6 pin) respectively, shaking table was cultivated 72 hours under 28 ℃ rotating speed 200rpm condition, obtain respectively the bacterium liquid of 7 kinds of bacterial strains, the content of the bacterium in every kind of bacterium liquid is respectively 3.1 * 10
7Cfu/ml, 2.1 * 10
8Cfu/ml, 2.46 * 10
7Cfu/ml, 4.9 * 10
8Cfu/ml, 4.23 * 10
7Cfu/ml, 2.03 * 10
8Cfu/ml, 1.83 * 10
8Cfu/ml.
3, solid enlarges fermentation culture
Respectively with each bacterial strain bacterium liquid in mass ratio equal proportion mix, make composite bacteria liquid;
With composite bacteria liquid by volume the inoculum size of per-cent 4% be linked in the solid enlarged culturing base, add the water stirring and be adjusted to water content 55%(quality percentage composition), cultivate at 28 ℃ condition bottom fermentations, the culture base-material stacking height is 10cm, stir every day 1 time, finish through 8 days fermentation culture, obtain tunning.
4, drying
Tunning is dried to moisture content 16% at 45 ℃; Obtain composite fungus agent.
5, detect
The composite fungus agent goods that gather different depths detect, and the living bacteria count of composite fungus agent is 3.2 * 10
8Cfu/g, moisture content is 15.4%.
6, packing, preservation
Adopt opaque plastics bag to pack, every bag of 0.5Kg places the environment of drying, cool place, ventilation to preserve, and preserving and detecting viable count after 6 months is 2.8 * 10
8Cfu/g.
The preparation of embodiment 2, composite fungus agent and detection
1, slant strains is cultivated
Thermophilic fungus destroyed wire (Sporotrichum thermophile) preserving number CICC2441, healthy and free from worry wood mould (Trichoderma konigii) preserving number CGMCC3.4001, whiterot fungi (Phanerochaete chrysosporium) preserving number CGMCC5.776, aspergillus niger (Aspergillus niger) preserving number CGMCC3.03147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) preserving number CICC1415, subtilis (Bacillus subtilis) preserving number CICC10076, producing these 7 kinds of bacterial strains of dyadic streptomycete (Streptomyces ambofaciens) preserving number ACCC40133 and be inoculated in respectively on the corresponding slant medium, is to leave standstill under 30 ℃ the condition to cultivate 24 hours in temperature.
2, liquid seeds enlarged culturing
Each bacterial strain behind the picking slant culture is inoculated respectively (1L culture medium inoculated 4-6 pin) in corresponding liquid nutrient medium respectively, shaking table is 84 hours under 30 ℃ rotating speed 200rpm condition, obtain respectively the bacterium liquid of 7 kinds of bacterial strains, the content of the bacterium in every kind of bacterium liquid is respectively 3.8 * 10
7Cfu/ml, 3.1 * 10
8Cfu/ml, 4.0 * 10
7Cfu/ml, 6.22 * 10
8Cfu/ml, 5.83 * 10
7Cfu/ml, 4.51 * 10
8Cfu/ml and 2.65 * 10
8Cfu/ml.
3, solid enlarges fermentation culture
Respectively with each bacterial strain bacterium liquid in mass ratio equal proportion mix, make composite bacteria liquid;
With composite bacteria liquid by the long-pending per-cent of quality volume percent 5%(composite bacteria liquid quality and solid enlarged culturing matrix) inoculum size be linked in the solid enlarged culturing base, add the water stirring and be adjusted to water content 60%(quality percentage composition), cultivate at 35 ℃ condition bottom fermentations, the culture base-material stacking height is 14cm, stir every day 2 times, finish through 5 days fermentation culture, obtain tunning.
4, drying
Tunning is dried to moisture content 12% at 45 ℃; Obtain composite fungus agent.
5, detect
The composite fungus agent goods that gather different depths detect, and the living bacteria count of composite fungus agent is 5.2 * 10
8Cfu/g, moisture content is 13.6%.
6, packing, preservation
Adopt opaque plastics bag to pack, every bag of 1Kg places the environment of drying, cool place, ventilation to preserve, and preserving and detecting viable count after 6 months is 4.7 * 10
8Cfu/g.
Embodiment 3, composite fungus agent are used
Compost take manioc waste as main raw material, specific as follows:
Composting material forms: cassava alcohol slag (particle diameter is less than 2mm, and C/N is about 45) 75%(quality percentage composition) and filter mud (cassava alcohol factory waste water filter mud) 25%(quality percentage composition);
Making processes: add respectively the composite fungus agent that is obtained by embodiment 1 or embodiment 2 by 0.2% and 0.22% of composting material quality, regulating C/N with urea is 30, regulating water content is 60%(quality percentage composition), stir, pile the strip that height is no more than 80cm, the wide 150-200cm in bottom.Adopt artificial turning, enter every 3d turning of pliotherm period 1 time, cooldown period 5d turning 1 time obtains fertilizer.Take the same composting material that do not add composite fungus agent as blank, do positive control with 0.22% and 0.2% aspergillus niger and the single bacterium of thermophilic fungus destroyed wire that add respectively the composting material quality.
Above-mentioned fertilizer is carried out the compost maturity effect detection, and the result is as shown in table 1.
The detection method of cellulose decomposition rate:
(1) with filter cloth oven dry under 105 ℃, kept dry.
(2) the 1.0000g sample is placed the 150mL beaker, add 70ml2.0mol/L HCl, put into 100 ℃ of insulations of high-pressure steam sterilizing pan 50min.
(3) filter after HCl processes, filter residue washs 2 times successively with 95% ethanol, dehydrated alcohol and acetone, and filter residue and filter cloth are together dried, and being dried to constant weight, to deduct filter paper heavily be W
1
(4) filter cloth surface residue is swept in the beaker, add 10ml72%H
2SO
4, add 90ml distilled water behind the degraded 4h and spend the night.Filter next day, filter residue is washed till about pH6.5, and filter residue and filter cloth are together dried, and is dried to constant weight and deducts filter paper and heavily be W
2
Calculation formula is:
The detection method of humic acids content:
Manioc waste after the fermentation of becoming thoroughly decomposed of oven dry is ground into 40 orders, accurately about weighing 1.000g, adds 0.2mol/LNaOH50ml.Centrifugation behind constant-temperature table vibration 24h.The clear liquid of separating is placed in the triangular flask, adds 0.5mol/L HCl70ml.After leaving standstill 24h, again centrifugation.Remove the upper strata sour water, throw out is washed till without Cl with deionized water
-1Detect.Then humic acid precipitation is washed in the load weighted watch-glass, be placed in the loft drier freeze-day with constant temperature and weigh to constant weight.The black solid that finally obtains is exactly humic acid (HA) particle.Calculation formula is:
In the formula: W
1: humic acid is heavy, g;
W
0: sample is heavy, g.
Table 1 adds microbial inoculum to the promoter action of compost maturity
| Composting material | The cellulose decomposition rate | Humic acids content |
| Manioc waste+filter mud+0.2% composite fungus agent (embodiment 1) | 20.88％ | 10.08％ |
| Manioc waste+filter mud+0.22% composite fungus agent (embodiment 2) | 18.53％ | 9.78％ |
| Manioc waste+filter mud (blank) | 11.67％ | 8.11％ |
| Manioc waste+filter mud+0.22% black-koji mould (positive control) | 15.41％ | 7.82％ |
| Manioc waste+filter mud+0.2% thermophilic fungus destroyed wire bacterium (positive control) | 12.97％ | 7.83％ |
As can be seen from Table 1, cellulose decomposition and humic acids accumulation there is good promoter action after adding the composite fungus agent that is obtained by the present invention.
Claims (10)
1. microbial inoculum that promotes that composting material becomes thoroughly decomposed, its activeconstituents are thermophilic fungus destroyed wire (Sporotrichum thermophile), healthy and free from worry wood mould (Trichoderma konigii), whiterot fungi (Phanerochaete chrysosporium), aspergillus niger (Aspergillus niger), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), subtilis (Bacillus subtilis) and produce dyadic streptomycete (Streptomyces ambofaciens).
2. the microbial inoculum described in according to claim 1, it is characterized in that: the activeconstituents of described microbial inoculum is thermophilic fungus destroyed wire (Sporotrichum thermophile) CICC2441, healthy and free from worry wood mould (Trichoderma konigii) CGMCC3.04001, whiterot fungi (Phanerochaete chrysosporium) CGMCC5.00776, aspergillus niger (Aspergillus niger) CGMCC3.3147, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC1415, subtilis (Bacillus subtilis) CICC10076 and product dyadic streptomycete (Streptomyces ambofaciens) ACCC40133.
3. the microbial inoculum described in according to claim 1 and 2, it is characterized in that: effectively total viable count of described microbial inoculum is greater than 0.5 * 10
8Cfu/g; Wherein, the ratio of the living bacteria count of described thermophilic fungus destroyed wire, mould, the described whiterot fungi of described healthy and free from worry wood, described aspergillus niger, described yeast saccharomyces cerevisiae, described subtilis and described product dyadic streptomycete is 1:(1-30): (0.1-5): (1-50): (0.1-5): (1-50): (1-30);
Described thermophilic fungus destroyed wire is that the mould mould CGMCC3.04001 of healthy and free from worry wood of being of thermophilic fungus destroyed wire CICC2441, described healthy and free from worry wood, described whiterot fungi are that whiterot fungi CGMCC5.00776, described aspergillus niger are that aspergillus niger CGMCC3.3147, described yeast saccharomyces cerevisiae are that yeast saccharomyces cerevisiae CICC1415, described subtilis are that subtilis CICC10076 and described product dyadic streptomycete are product dyadic streptomycete ACCC40133.
4. according to claim 1 and 2 or the microbial inoculum described in 3, it is characterized in that: the quality percentage composition of moisture is 12-16% in the described microbial inoculum; The living bacteria count of described microbial inoculum is 2.8 * 10
8Cfu/g-5.2 * 10
8Cfu/g;
Further, the living bacteria count of described microbial inoculum is 3.2 * 10
8Cfu/g-5.2 * 10
8Cfu/g;
Further, the living bacteria count of described microbial inoculum is specially 2.8 * 10
8Cfu/g, 3.2 * 10
8Cfu/g, 4.7 * 10
8Cfu/g or 5.2 * 10
8Cfu/g.
5. a method for preparing arbitrary described microbial inoculum among the claim 1-4 comprises the steps:
1) following 7 kinds of bacteria culture fluids is mixed, obtain composite bacteria liquid: thermophilic fungus destroyed wire (Sporotrichum thermophile) bacteria culture fluid, healthy and free from worry wood mould (Trichoderma konigii) bacteria culture fluid, whiterot fungi (Phanerochaete chrysosporium) bacteria culture fluid, aspergillus niger (Aspergillus niger) bacteria culture fluid, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) bacteria culture fluid, subtilis (Bacillus subtilis) bacteria culture fluid and product dyadic streptomycete (Streptomyces ambofaciens) bacteria culture fluid;
2) mix adding water behind the described composite bacteria liquid access solid enlarged culturing base, obtain culture base-material, the described culture base-material of fermentation culture is collected tunning, namely obtains microbial inoculum.
6. method according to claim 5 is characterized in that:
In the step 1), the living bacteria count of described 7 kinds of bacteria culture fluids is all greater than 1 * 10
7Cfu/ml;
The mass ratio of described 7 kinds of bacteria culture fluids is followed successively by: 1:(0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1): (0.9-1.1);
Described thermophilic fungus destroyed wire is that the mould mould CGMCC3.04001 of healthy and free from worry wood of being of thermophilic fungus destroyed wire CICC2441, described healthy and free from worry wood, described whiterot fungi are that whiterot fungi CGMCC5.00776, described aspergillus niger are that aspergillus niger CGMCC3.3147, described yeast saccharomyces cerevisiae are that yeast saccharomyces cerevisiae CICC1415, described subtilis are that subtilis CICC10076 and described product dyadic streptomycete are product dyadic streptomycete ACCC40133;
Step 2) in, described composite bacteria liquid is to add water behind the inoculum size access solid enlarged culturing base of 4%-5% to be mixed to biodiversity per-cent be 55%-60% by the quality volume percent.
7. it is characterized in that according to claim 5 or 6 described methods:
In the step 1), the living bacteria count of described 7 kinds of bacteria culture fluids respectively is 1.95-4.6 * 10
7Cfu/ml, 0.87-5.83 * 10
8Cfu/ml, 1.26-7.63 * 10
7Cfu/ml, 1.4-9.47 * 10
8Cfu/ml, 0.87-9.57 * 10
7Cfu/ml, 1.52-8.8 * 10
8Cfu/ml and 1.39-4.67 * 10
8Cfu/ml;
Described 7 kinds of mass mixings such as bacteria culture fluid;
The culture condition that obtains of described 7 kinds of bacteria culture fluids is: cultivate 72-84h under 28-30 ℃, 200rpm condition;
Step 2) in, described solid enlarged culturing base is prepared as follows: with the cassava starch dregs of particle diameter 0.425-0.85mm, add in mass ratio 10% Semen Maydis powder, mixing obtains solid enlarged culturing base;
The condition of described fermentation culture is specific as follows: 28-35 ℃, described culture base-material stacking height be stir 10-14cm, every day 1-2 time, through 5-8 days fermentation culture, obtain tunning.
8. arbitrary described method according to claim 5-7 is characterized in that:
Step 2) in, the condition of the described culture base-material of described fermentation culture is as follows: 28-35 ℃, described culture base-material stacking height be stir 10-14cm, every day 1-2 time, through 5-8 days fermentation culture, obtain tunning;
Be specially:
28 ℃, described culture base-material stacking height be stir 10cm, every day 1 time, through 8 days fermentation culture, obtain tunning;
Or 35 ℃, described culture base-material stacking height be stir 14cm, every day 2 times, through 5 days fermentation culture, obtain tunning;
In step 2) the collection tunning after, comprise the steps: that also it is 12-16% that tunning is dried to the biodiversity percentage amounts, obtains microbial inoculum.
9. the application of arbitrary described microbial inoculum in production biological organic fertilizer, compost or promotion composting material become thoroughly decomposed among the claim 1-3.
10. application according to claim 8 is characterized in that:
Described composting material is organic waste; Described organic waste is the cellulose family organic waste; Described cellulose family organic waste is that 75% cassava alcohol slag and quality percentage composition are that 25% filter mud forms by the quality percentage composition;
Described composting material is 450-550:1 with the functional quality ratio of described microbial inoculum;
Described promotion composting material becomes thoroughly decomposed for promoting cellulose decomposition and/or improving humic acids content.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105330356A (en) * | 2015-11-20 | 2016-02-17 | 四川北方硝化棉股份有限公司 | Waste nitrocotton treating method |
| CN108587967A (en) * | 2018-04-30 | 2018-09-28 | 黄山中科新佳生物科技有限公司 | The preparation method and applications of the decomposed composite bacteria agent of kitchen garbage of high temperature resistant salt tolerant |
| CN109055263A (en) * | 2018-08-13 | 2018-12-21 | 西南科技大学 | The antimicrobial composition and biodegrading process of degradation heavy metal accumulation plant |
| CN109679874A (en) * | 2019-01-15 | 2019-04-26 | 青岛科信新能源技术有限公司 | A method of biomass thermal liquid waste solution is handled using microbial strains |
| CN112205514A (en) * | 2020-09-29 | 2021-01-12 | 天津科技大学 | Method for producing multifunctional spirit vinasse feed |
| CN113582777A (en) * | 2021-09-08 | 2021-11-02 | 重庆市风景园林科学研究院 | Fermentation inoculant and application technology thereof in garden plant waste composting |
| CN114015597A (en) * | 2021-11-04 | 2022-02-08 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating garden residual branch organic garbage |
| CN114058540A (en) * | 2021-11-04 | 2022-02-18 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating organic garbage of fruits and vegetables |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174398A (en) * | 2010-12-14 | 2011-09-07 | 北京沃土天地生物科技有限公司 | Composite microbiological bacterial agent used for returning maize straws to field and preparation method and applications thereof |
| CN102178045A (en) * | 2011-06-23 | 2011-09-14 | 南开大学 | Method for producing feed by fermenting and ammoniating straws |
| CN102212494A (en) * | 2011-04-12 | 2011-10-12 | 王颖 | Organic matter decomposing inoculant, and preparation method and application thereof |
| CN102224881A (en) * | 2011-06-23 | 2011-10-26 | 南开大学 | Ammonified straw and distillers' grains mixed fermented feed and production method thereof |
| CN102276301A (en) * | 2011-06-02 | 2011-12-14 | 武汉金禾科技发展有限公司 | Straw decomposing agent and production method thereof |
| CN102337237A (en) * | 2011-09-29 | 2012-02-01 | 北京可力美施生物科技有限公司 | Functional organic material decomposing agent and preparation method thereof |
| CN102382768A (en) * | 2011-09-29 | 2012-03-21 | 武汉施瑞福生物技术有限公司 | Microbial straw decomposition additive as well as preparation method and application thereof |
| CN102517217A (en) * | 2011-12-27 | 2012-06-27 | 何颖霞 | Environment-friendly straw fermenting agent |
-
2013
- 2013-07-12 CN CN201310292272.9A patent/CN103320369B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174398A (en) * | 2010-12-14 | 2011-09-07 | 北京沃土天地生物科技有限公司 | Composite microbiological bacterial agent used for returning maize straws to field and preparation method and applications thereof |
| CN102212494A (en) * | 2011-04-12 | 2011-10-12 | 王颖 | Organic matter decomposing inoculant, and preparation method and application thereof |
| CN102276301A (en) * | 2011-06-02 | 2011-12-14 | 武汉金禾科技发展有限公司 | Straw decomposing agent and production method thereof |
| CN102178045A (en) * | 2011-06-23 | 2011-09-14 | 南开大学 | Method for producing feed by fermenting and ammoniating straws |
| CN102224881A (en) * | 2011-06-23 | 2011-10-26 | 南开大学 | Ammonified straw and distillers' grains mixed fermented feed and production method thereof |
| CN102337237A (en) * | 2011-09-29 | 2012-02-01 | 北京可力美施生物科技有限公司 | Functional organic material decomposing agent and preparation method thereof |
| CN102382768A (en) * | 2011-09-29 | 2012-03-21 | 武汉施瑞福生物技术有限公司 | Microbial straw decomposition additive as well as preparation method and application thereof |
| CN102517217A (en) * | 2011-12-27 | 2012-06-27 | 何颖霞 | Environment-friendly straw fermenting agent |
Non-Patent Citations (4)
| Title |
|---|
| 周新萍 等: "高产纤维素酶生二素链霉菌的鉴定与选育研究", 《CHINA BREWING》, no. 3, 31 March 2007 (2007-03-31), pages 20 - 26 * |
| 文少白 等: "微生物降解纤维素的研究概况", 《中国农学通报》, vol. 26, no. 1, 31 January 2010 (2010-01-31), pages 231 - 236 * |
| 王宝林 等: "一株嗜热毁丝霉菌株产纤维素酶条件优化", 《酿酒科技》, no. 10, 30 October 2012 (2012-10-30), pages 27 - 31 * |
| 郑鹏 等: "8个菌株的纤维素分解能力比较研究", 《热带作物学报》, vol. 33, no. 6, 30 June 2012 (2012-06-30), pages 1121 - 1125 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105330356A (en) * | 2015-11-20 | 2016-02-17 | 四川北方硝化棉股份有限公司 | Waste nitrocotton treating method |
| CN108587967A (en) * | 2018-04-30 | 2018-09-28 | 黄山中科新佳生物科技有限公司 | The preparation method and applications of the decomposed composite bacteria agent of kitchen garbage of high temperature resistant salt tolerant |
| CN109055263A (en) * | 2018-08-13 | 2018-12-21 | 西南科技大学 | The antimicrobial composition and biodegrading process of degradation heavy metal accumulation plant |
| CN109679874A (en) * | 2019-01-15 | 2019-04-26 | 青岛科信新能源技术有限公司 | A method of biomass thermal liquid waste solution is handled using microbial strains |
| CN112205514A (en) * | 2020-09-29 | 2021-01-12 | 天津科技大学 | Method for producing multifunctional spirit vinasse feed |
| CN113582777A (en) * | 2021-09-08 | 2021-11-02 | 重庆市风景园林科学研究院 | Fermentation inoculant and application technology thereof in garden plant waste composting |
| CN114015597A (en) * | 2021-11-04 | 2022-02-08 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating garden residual branch organic garbage |
| CN114058540A (en) * | 2021-11-04 | 2022-02-18 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating organic garbage of fruits and vegetables |
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