CN105943833B - Anti-fatigue traditional Chinese medicine health tea for physical exercise - Google Patents
Anti-fatigue traditional Chinese medicine health tea for physical exercise Download PDFInfo
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Abstract
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: the anti-fatigue health-care tea is characterized by comprising, by weight, 10-20 parts of ginseng, 5-15 parts of prepared rehmannia root, 5-15 parts of dogwood fruit, 10-14 parts of Chinese yam and 5-15 parts of asparagus, and is used for sports anti-fatigue traditional Chinese medicine health-care tea.
Description
Technical Field
The invention belongs to the field of physical strength recovery and fatigue resistance in physical exercise, and particularly relates to anti-fatigue traditional Chinese medicine health-care tea in physical exercise. In addition, the invention also relates to an anti-fatigue action mechanism and aging delaying of the anti-fatigue traditional Chinese medicine health tea for physical exercise.
Background
In physical recovery from physical exercise for fatigue resistance, fatigue is a physiological phenomenon in which the body function does not continue at a specific level or the whole body fails to maintain a predetermined exercise intensity, and is an extremely complex process of physical change. Fatigue can cause the athletic ability of sports athletes to be reduced, if fatigue cannot be eliminated in time after the occurrence of fatigue, the fatigue can be gradually accumulated, and finally strain is caused, and overtraining syndrome, chronic fatigue syndrome and the like appear, so that endocrine disturbance and immunity decline of an organism are caused, aging is easy to happen, even organic lesion appears, and the fatigue becomes an important factor threatening physical and mental health of human beings. Therefore, delaying the onset of fatigue and promoting recovery from fatigue have been the research focus of the sports recovery discipline.
Sugars are an important energy source for muscle tissue. Most of the energy of muscle tissues is supplied by sugar metabolism when the body performs a short-time heavy exercise, and energy is supplied by sugar oxidation first when a long-time light exercise is performed, and fat and protein are used when available sugar is exhausted. Sugar is most easily oxidized, oxygen consumption is low during oxidation, and compared with fat, under the condition of consuming equal amount of oxygen, the yield efficiency of sugar is higher than that of fat by 4.5 percent, which is more important under the condition of insufficient oxygen and can be a factor for determining win and loss at some time of a match. In sports for more than 1 hour, such as long distance running, long distance swimming, cycling, skiing, marathon, triathlon, football, hockey, tennis, etc., sugar reserves in the body can be depleted, and glycogen depletion can affect athletic performance, particularly endurance. Sugar stores in the body include three classes of muscle glycogen, liver glycogen and blood glucose. Muscle glycogen is the largest part of the sugar reserve. Reserve and metabolic capacity of muscle glycogen are important factors determining endurance. Numerous studies have shown that physical failure due to exercise always occurs simultaneously with depletion of muscle glycogen. With the increasing consumption of muscle glycogen, the body will consume liver glycogen to maintain blood glucose levels, resulting in a decrease in liver glycogen. Thus, liver glycogen and muscle glycogen content are sensitive indicators reflecting the degree of fatigue.
Exercise-induced fatigue is an unavoidable physiological phenomenon caused by exercise. Delaying the onset of fatigue and promoting recovery from fatigue have been the focus of research in sports medicine. Since chemicals and biological products contain components that may cause harm to the body and thus their use is limited, there is a strong need to find safer and more effective anti-fatigue materials. The physical recovery, fatigue resistance and aging delay of sports are realized to the maximum extent, and the development of the traditional Chinese medicine as a sports tonic becomes a hot topic of sports medicine research.
Disclosure of Invention
The invention aims to provide a novel traditional Chinese medicine health tea with an anti-fatigue effect. Specifically, the invention provides the traditional Chinese medicine health tea with the anti-fatigue effect, which comprises the following medicinal components:
the invention aims to provide a novel traditional Chinese medicine health-care tea with anti-fatigue activity.
The second purpose of the invention is to provide the anti-fatigue pharmacological action and action mechanism of the novel traditional Chinese medicine health-care tea.
A traditional Chinese medicine health tea for resisting fatigue in sports comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, oriental waterplantain rhizome, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 10 to 20 parts by weight, the prepared rehmannia root comprises 5 to 15 parts by weight, the dogwood fruit comprises 5 to 15 parts by weight, the Chinese yam comprises 10 to 14 parts by weight, the asparagus root comprises 5 to 15 parts by weight, the dwarf lilyturf tuber comprises 5 to 15 parts by weight, the hawthorn fruit comprises 5 to 15 parts by weight, the tuckahoe comprises 5 to 15 parts by weight, the cortex moutan is 5 to 15 parts by weight, the oriental waterplantain rhizome comprises 5 to 15 parts by weight, the schisandra fruit comprises 5 to 15 parts by weight, and the maltose comprises 8, 6-10 parts of fried semen cuscutae, 6-15 parts of wolfberry fruit, 5-10 parts of deerhorn glue, 6-15 parts of kudzu root, 5-10 parts of prepared fleece-flower root, 5-10 parts of paper mulberry fruit, 5-10 parts of flatstem milkvetch seed and 5-10 parts of honey-fried licorice root.
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, alisma orientale, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 10 parts by weight, the prepared rehmannia root comprises 5 parts by weight, the dogwood comprises 5 parts by weight, the yam comprises 10 parts by weight, the asparagus root comprises 5 parts by weight, the dwarf lilyturf tuber comprises 5 parts by weight, the hawthorn fruit comprises 5 parts by weight, the tuckahoe comprises 5 parts by weight, the cortex moutan comprises 5 parts by weight, the alisma rhizome comprises 5 parts by weight, the schisandra fruit comprises 5 parts by weight, the maltose comprises 8 parts by weight, the fried dodder seed comprises 6 parts by weight, the medlar comprises 6 parts by weight, the deerhorn glue comprises 5 parts, 6 parts of kudzu root, 5 parts of prepared fleece-flower root, 5 parts of papermulberry fruit, 5 parts of flatstem milkvetch seed and 5 parts of honey-fried licorice root.
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, alisma orientale, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 20 parts by weight, the prepared rehmannia root comprises 15 parts by weight, the dogwood comprises 15 parts by weight, the yam comprises 14 parts by weight, the asparagus root comprises 15 parts by weight, the dwarf lilyturf tuber comprises 15 parts by weight, the hawthorn fruit comprises 15 parts by weight, the tuckahoe comprises 15 parts by weight, the cortex moutan comprises 15 parts by weight, the alisma rhizome comprises 15 parts by weight, the schisandra fruit comprises 15 parts by weight, the maltose comprises 14 parts by weight, the fried dodder seed comprises 10 parts by weight, the medlar comprises 15 parts by weight, the deerhorn glue comprises 10 parts, the radix puerariae beverage is prepared from 15 parts by weight of radix puerariae, 10 parts by weight of radix polygoni multiflori preparata, 10 parts by weight of fructus broussonetiae, 10 parts by weight of semen astragali complanati and 10 parts by weight of radix glycyrrhizae preparata.
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, alisma orientale, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 15 parts by weight, the prepared rehmannia root comprises 10 parts by weight, the dogwood comprises 10 parts by weight, the yam comprises 12 parts by weight, the asparagus root comprises 8 parts by weight, the dwarf lilyturf tuber comprises 8 parts by weight, the hawthorn fruit comprises 10 parts by weight, the tuckahoe comprises 10 parts by weight, the cortex moutan comprises 10 parts by weight, the alisma rhizome comprises 10 parts by weight, the schisandra fruit comprises 10 parts by weight, the maltose comprises 10 parts by weight, the fried dodder seed comprises 8 parts by weight, the medlar comprises 10 parts by weight, the deerhorn glue comprises 8 parts, the radix puerariae beverage is prepared from 10 parts by weight of radix puerariae, 8 parts by weight of radix polygoni multiflori preparata, 8 parts by weight of fructus broussonetiae, 8 parts by weight of semen astragali complanati and 6 parts by weight of radix glycyrrhizae preparata.
Advantageous effects
In the invention, the prescription of the compound traditional Chinese medicine is firstly studied on the anti-fatigue traditional Chinese medicine health tea for sports by taking the drug effect as an index, and the prescription composition is finally determined. Establishing a mouse forced swimming experimental model, taking the mouse exhaustion swimming time as an index, confirming that the sports anti-fatigue traditional Chinese medicine health-care tea has obvious anti-fatigue effect (see an example 1 for details), simultaneously, utilizing the liver glycogen, muscle glycogen, lactate dehydrogenase and serum glucose content of a mouse after sports as indexes to research the anti-fatigue effect mechanism of the sports anti-fatigue traditional Chinese medicine health-care tea (see an example 2 for details), measuring the contents of the lactate dehydrogenase and the serum glucose by a full-automatic biochemical analyzer, and measuring the contents of the liver glycogen and the muscle glycogen by an ultraviolet spectrophotometer. The effective components of the sports anti-fatigue traditional Chinese medicine health tea provided by the invention are in a forced swimming experiment model of mice, the exhaustion swimming time of mice in a medium-dose group and a high-dose group is obviously longer than that of mice in a control group, and the sports anti-fatigue traditional Chinese medicine health tea has better anti-fatigue activity. In the research of the anti-fatigue action mechanism, the liver glycogen and the muscle glycogen of a mouse in a drug group are obviously higher than those of a control group after exercise, and the anti-fatigue traditional Chinese medicine health tea for physical exercise is prompted to play an important role in maintaining the liver glycogen and the muscle glycogen content of the mouse after exercise, so that a better energy reserve is provided for an organism. Simultaneously, the content of serum glucose and lactate dehydrogenase can be increased, and the activity of the lactate dehydrogenase is dose-dependent. The composition is shown to have remarkable anti-fatigue effect, and meanwhile, the composition is also found to have certain anti-aging effect in example 3; therefore, the invention has better effects of resisting fatigue and delaying senility, does not have any side effect and has good effect.
The traditional Chinese medicine health tea for physical exercise and fatigue resistance is sweet and mild in temperature, good for invigorating primordial qi and promoting the production of body fluid, and is an essential drug for treating consumptive disease and internal injury; prepared rehmannia root, radix rehmanniae is sweet and slightly warm and has the effect of nourishing yin, tonifying kidney, nourishing blood and replenishing essence to consolidate the constitution. They are combined to nourish yin and tonify qi, strengthen the body resistance and reinforce primordial qi, so they are monarch drugs. The wine-roasted dogwood warms and tonifies and astringes, and is good at tonifying the kidney, nourishing the liver, arresting seminal emission and absorbing qi; the yam is sweet and neutral and can tonify the astringency, benefit qi and nourish yin, secure essence and reduce urination and receive qi; ophiopogon japonicus is sweet and slightly bitter, has slight cold and clear and nourish, and is good at nourishing yin and moistening dryness, clearing heart fire and relieving restlessness; tian Dong is sweet, moist, nourishing, bitter and cold, clearing and descending, good at clearing and nourishing lung and kidney, and can nourish yin, clear fire, moisten dryness and smooth intestine. The four medicines are matched to help the monarch medicine to nourish and consolidate the constitution, and also can astringe and discharge the lower yuan, clear away the heart fire and relieve restlessness, and relax bowel, so the traditional Chinese medicine is a ministerial medicine. Alisma orientale is sweet, cold and clear, good at purging heat and promoting diuresis, and combined with prepared rehmannia root for purging kidney and descending turbidity; poria cocos, rhizoma Dioscoreae, Poria cocos, rhizoma Dioscoreae, Poria cocos; cortex moutan, being bitter and cold in property, is used to clear deficiency heat, and combined with Tian and Mai Dong to make shan Zhu Yu stir-baked with wine warm and astringent. The kudzu vine root is pungent and cool, and can stimulate the spleen and stomach to clear up the ascending of yang qi to produce the body fluid and quench thirst; the parched dodder seed is pungent, sweet, mild and astringent, and is good at nourishing yin and tonifying yang, nourishing liver and improving eyesight, and securing essence and reducing urination; gou Qi Zi is sweet, neutral and moist in nature, good at nourishing Yin and tonifying kidney, and also good at supporting kidney yang. The medicines are combined to tonify the middle-jiao and discharge, promote the recovery of true yin and strengthen the effect of monarch and ministerial medicines, so the traditional Chinese medicine is an adjuvant medicine. Prepared licorice root, radix Glycyrrhizae Praeparata, sweet in taste and neutral in nature, acts as a guiding drug for tonifying middle-jiao and harmonizing various herbs in the recipe.
Ginseng radix is dried root of Panaxginsen C.A.Mey. of Araliaceae, and is recorded in Shen nong's herbal Jing for nourishing five internal organs, calming spirit, stopping palpitation, eliminating pathogenic qi, improving eyesight, improving intelligence, reducing weight, and prolonging life. The ginseng has complex chemical components, contains saponin, fatty acid, volatile oil, amino acid, saccharide, flavone, vitamin and the like, wherein the saponin components are considered as main active components in the ginseng, and have good effects on resisting tumors, resisting aging, resisting arrhythmia, improving learning and memory, improving microcirculation, improving the anti-hypoxia capability of tissues, inhibiting platelet aggregation, enhancing sexual function and immune function, resisting fatigue and the like. The results of pharmacological research, clinical observation and epidemiological investigation on the effective components of ginseng by internal and external students prove that the ginsenoside can relieve physical fatigue through the functions of resisting oxygen free radicals, regulating energy substances, regulating lactic acid metabolism and the like, and has the functions of improving training performance, improving maximum oxygen intake, stabilizing internal environment and reducing serum lactic acid after exercise.
At present, no research report of health-care beverage capable of playing an anti-fatigue effect from multiple levels exists, the inventor finally obtains a traditional Chinese medicine health-care tea with a remarkable anti-fatigue effect through long-term and hard research experiments, and the pharmacological activity detection shows that the overall synergistic effect of the traditional Chinese medicine health-care tea has good anti-fatigue activity and can delay senility. After the composition is taken by athletes, no side effect occurs, and the effect is good.
Detailed Description
A traditional Chinese medicine health tea for resisting fatigue in sports comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, oriental waterplantain rhizome, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 10 to 20 parts by weight, the prepared rehmannia root comprises 5 to 15 parts by weight, the dogwood fruit comprises 5 to 15 parts by weight, the Chinese yam comprises 10 to 14 parts by weight, the asparagus root comprises 5 to 15 parts by weight, the dwarf lilyturf tuber comprises 5 to 15 parts by weight, the hawthorn fruit comprises 5 to 15 parts by weight, the tuckahoe comprises 5 to 15 parts by weight, the cortex moutan is 5 to 15 parts by weight, the oriental waterplantain rhizome comprises 5 to 15 parts by weight, the schisandra fruit comprises 5 to 15 parts by weight, and the maltose comprises 8, 6-10 parts of fried semen cuscutae, 6-15 parts of wolfberry fruit, 5-10 parts of deerhorn glue, 6-15 parts of kudzu root, 5-10 parts of prepared fleece-flower root, 5-10 parts of paper mulberry fruit, 5-10 parts of flatstem milkvetch seed and 5-10 parts of honey-fried licorice root.
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, alisma orientale, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 10 parts by weight, the prepared rehmannia root comprises 5 parts by weight, the dogwood comprises 5 parts by weight, the yam comprises 10 parts by weight, the asparagus root comprises 5 parts by weight, the dwarf lilyturf tuber comprises 5 parts by weight, the hawthorn fruit comprises 5 parts by weight, the tuckahoe comprises 5 parts by weight, the cortex moutan comprises 5 parts by weight, the alisma rhizome comprises 5 parts by weight, the schisandra fruit comprises 5 parts by weight, the maltose comprises 8 parts by weight, the fried dodder seed comprises 6 parts by weight, the medlar comprises 6 parts by weight, the deerhorn glue comprises 5 parts, 6 parts of kudzu root, 5 parts of prepared fleece-flower root, 5 parts of papermulberry fruit, 5 parts of flatstem milkvetch seed and 5 parts of honey-fried licorice root.
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, alisma orientale, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 20 parts by weight, the prepared rehmannia root comprises 15 parts by weight, the dogwood comprises 15 parts by weight, the yam comprises 14 parts by weight, the asparagus root comprises 15 parts by weight, the dwarf lilyturf tuber comprises 15 parts by weight, the hawthorn fruit comprises 15 parts by weight, the tuckahoe comprises 15 parts by weight, the cortex moutan comprises 15 parts by weight, the alisma rhizome comprises 15 parts by weight, the schisandra fruit comprises 15 parts by weight, the maltose comprises 14 parts by weight, the fried dodder seed comprises 10 parts by weight, the medlar comprises 15 parts by weight, the deerhorn glue comprises 10 parts, the radix puerariae beverage is prepared from 15 parts by weight of radix puerariae, 10 parts by weight of radix polygoni multiflori preparata, 10 parts by weight of fructus broussonetiae, 10 parts by weight of semen astragali complanati and 10 parts by weight of radix glycyrrhizae preparata.
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, alisma orientale, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, and is characterized in that the ginseng comprises 15 parts by weight, the prepared rehmannia root comprises 10 parts by weight, the dogwood comprises 10 parts by weight, the yam comprises 12 parts by weight, the asparagus root comprises 8 parts by weight, the dwarf lilyturf tuber comprises 8 parts by weight, the hawthorn fruit comprises 10 parts by weight, the tuckahoe comprises 10 parts by weight, the cortex moutan comprises 10 parts by weight, the alisma rhizome comprises 10 parts by weight, the schisandra fruit comprises 10 parts by weight, the maltose comprises 10 parts by weight, the fried dodder seed comprises 8 parts by weight, the medlar comprises 10 parts by weight, the deerhorn glue comprises 8 parts, the radix puerariae beverage is prepared from 10 parts by weight of radix puerariae, 8 parts by weight of radix polygoni multiflori preparata, 8 parts by weight of fructus broussonetiae, 8 parts by weight of semen astragali complanati and 6 parts by weight of radix glycyrrhizae preparata.
An anti-fatigue traditional Chinese medicine health tea for sports, which comprises the following components: 15g of ginseng, 10g of prepared rehmannia root, 10g of dogwood, 12g of Chinese yam, 8g of asparagus, 8g of dwarf lilyturf tuber, 10g of hawthorn, 10g of tuckahoe, 10g of cortex moutan, 10g of rhizoma alismatis, 10g of Chinese magnoliavine fruit, 10g of maltose, 8g of fried dodder, 10g of medlar, 8g of deerhorn gelatin, 10g of kudzu root, 8g of prepared fleece-flower root, 8g of fructus broussonetiae, 8g of flastem milkvetch seed and 6g of honey-fried licorice root.
Example 1 forced swimming experiment- -ZYBJC anti-fatigue Activity verification
Male ICR mice, 11-12 weeks old, were placed in animal chambers alternating at room temperature 23 + -1 deg.C for 12h light and 12h night, and were free to eat and drink water. All experimental procedures were in compliance with the ethical committee standards for laboratory animals. Mice were fed ad-hoc for l weeks, randomly grouped (18 per group) as follows: model group (mice underwent forced swim experiments with physiological saline administration); in groups 2-4, after the mice are forced to swim, 75,150,300mg/Kg of sports anti-fatigue traditional Chinese medicine health tea (ZYBJC) is respectively given, and the administration volume is 10ml/Kg of body weight. The administration is carried out by intragastric administration once a day for 15 days. Forced swim experiments have been demonstrated as an effective animal model for the evaluation of anti-fatigue drugs. The forced swimming test was performed by placing the ICR mouse alone in a plastic cylinder (25 cm high, 10cm diameter) containing water at a temperature of 23-25 deg.C and a depth of 10cm high to ensure that the mouse could not escape and could not approach the bottom of the plastic cylinder. After each swimming, the mice were wiped dry with a paper towel and returned to the rearing cage. The water in the container needs to be replaced after each mouse swims. The mouse was forced to stay in the plastic cylinder for 6min, and when the mouse stopped struggling in the plastic cylinder, it was considered immobile while remaining floating (with only minor movements to keep the head out of the water). The total test time lasts for 6min, the first 2min is taken as an adaptation period, and the fixed time is recorded after the last 4 min. Forced swimming experiments were performed 30min after each day of dosing for 15d continuously, and the time for mice to swim exhaustively was recorded after the end of the last forced swimming.
The experimental results show that exhaustive swimming experiments are carried out after the administration for 15 days by gavage, and the results are shown in table 1. The exhaustive swimming time of the mice in the medium-dose group and the high-dose group is obviously longer than that of the mice in the control group, and the exhaustive swimming time of the mice in the low-dose group is statistically significant compared with that of the mice in the model control group. The data show that the sports anti-fatigue traditional Chinese medicine health tea can obviously prolong the exhaustion swimming time of mice, wherein the effect is most obvious in a high-dose group, and the anti-fatigue effect is better along with the increase of the dose. ZYBJC's representative ' sports antifatigue Chinese medicine health-care tea '
TABLE 1 Effect of ZYBJC on mouse exhaustive swimming time (X + -SD, n ═ 18)
Note that*P<0.05，**P<0.01
Example 2 study of ZYBJC mechanism for resisting fatigue
1. Effects on hepatic and muscle glycogen in mice after exercise:
the forced swimming experiment mouse is taken out and wiped dry after being infused with the stomach for 15d, and then is 30min after the last administration, the mouse is swimed for 90min without load, the mouse is killed after being quieted for 30min, the liver and the quadriceps femoris muscle of the mouse are taken out quickly, and the mouse is placed in a liquid nitrogen tank to be tested. The detection method comprises (1) rinsing liver or muscle specimen with normal saline, drying with filter paper, and accurately weighing tissue weight; (2) adding the samples into a test tube according to the weight (mg) of the samples and the volume (mul) of an alkali solution which is 1:3, boiling in a boiling water bath for 20mni, and cooling with running water; (3) further preparing glycogen hydrolysate into glycogen detection solution; mixing, decocting in boiling water for 5min, cooling, and measuring OD value of each tube at 620nm wavelength. Processing by SPSS 10.0 statistical software, wherein all data are expressed by X +/-S, and the difference between groups is analyzed by variance; p <0.05, significant difference; p <0.01, the difference was very significant.
The results of the effect of the anti-fatigue traditional Chinese medicine health tea (ZYBJC) for physical exercise on the liver glycogen and the muscle glycogen of the mice after exercise are shown in a table 2. After exercise, the muscle glycogen content of the mice in the low-dose group is higher than that of the control group (P <0.05), while the muscle glycogen content of the mice in the medium-dose group and the high-dose group is remarkably higher than that of the control group (P < 0.01); after exercise, the muscle glycogen content of the mice in the low-dose group and the medium-dose group is higher than that of the control group (P <0.05), and the liver glycogen content of the mice in the high-dose group is obviously higher than that of the control group (P < 0.01). The physical exercise anti-fatigue traditional Chinese medicine health tea (ZYBJC) is proved to be capable of obviously improving the contents of liver glycogen and muscle glycogen of mice after exercise. After the mice in the drug group take exercise, the liver glycogen and the muscle glycogen are both obviously higher than those in the control group, and the physical exercise anti-fatigue traditional Chinese medicine health tea is prompted to play an important role in maintaining the liver glycogen and the muscle glycogen content of the mice after exercise, thereby providing better energy storage for organisms.
ZYBJC's representative ' sports antifatigue Chinese medicine health-care tea '
TABLE 2 Effect of ZYBJC on liver and muscle glycogen in mice after exercise (X + -SD, n ═ 18)
Note that*P<0.05，**P<0.01
2. Effects on post-exercise serum glucose and lactate dehydrogenase:
after the forced swimming experiment mouse is subjected to the last experiment, the mouse is fished out and wiped dry by a paper towel, blood is collected from the inner canthus vein after 30min, the centrifugation is carried out at 4 ℃, the rpm is 3000 for 10min, and the contents of Lactate Dehydrogenase (LDH) and serum glucose (Glc) in serum are detected by a full-automatic biochemical analyzer. Processing by SPSS 10.0 statistical software, wherein all data are expressed by X +/-S, and the difference between groups is analyzed by variance; p <0.05, significant difference; p <0.01, the difference was very significant.
The results of the effect of the traditional Chinese medicine health tea (ZYBJC) for resisting fatigue in sports on the lactate dehydrogenase and the serum glucose content of the mice after sports are shown in a table 3. Compared with a model control group, the anti-fatigue traditional Chinese medicine health tea for physical exercise given with different doses can increase the content of Glc in the serum of the mouse, wherein the increase of the content of Glc with high dose has significant meaning compared with the model group. Meanwhile, the content of LDH in the serum of mice can be reduced by using different doses of the physical exercise anti-fatigue traditional Chinese medicine health tea, wherein the reduction of the LDH content in a high-dose group has a significant meaning compared with a model group. The result shows that the sports anti-fatigue traditional Chinese medicine health tea can increase the serum glucose and reduce the content of lactate dehydrogenase, and the activity of the tea has dose dependence.
Table 3 effect of zyjc on post-exercise mouse lactate dehydrogenase and serum glucose (X ± SD, n ═ 18)
Note that*P<0.05，**P<0.01
Example 3 Effect of ZYBJC on anti-aging Functions in mice
10-month-old ICR mice were placed in animal chambers alternating at room temperature 23 + -1 deg.C for 12h light and 12h night, and were free to eat and drink water. All experimental procedures were in compliance with the ethical committee standards for laboratory animals. Mice were fed ad-hoc for l weeks, randomly grouped (15 per group) as follows: blank control, groups 2-4: in the treatment group, 75,150,300mg/Kg of physical exercise anti-fatigue traditional Chinese medicine health tea (ZYBJC) is respectively given, and the administration volume is 10ml/Kg of body weight. The administration is carried out by intragastric administration once a day for 60 days continuously, and various aging indexes are measured. ZYBJC's representative ' sports antifatigue Chinese medicine health-care tea '
1. Determination of lipofuscin content in myocardium
Collecting myocardium, removing connective tissue and fat, accurately weighing, adding chloroform methanol extractive solution (2:1) into homogenizer, homogenizing, and extracting lipofuscin for 2-3 times. The several extracts were combined and made up to 7 ml. The extract was centrifuged at 3000rpm for 10min, and the supernatant was used for measuring fluorescence intensity. The excitation wavelength is 360nm, the emission wavelength is 450nm, the fluorescence intensity is 50 units, quinine sulfate (0.1 mu g/ml,0.1mol/L sulfuric acid) is used as a standard control, a sample is measured, chloroform methanol extract is used as a blank control, and the experimental result is shown in Table 4.
TABLE 4 Effect of ZYBJC on the lipofuscin content in the myocardium of mice (X + -SD, n 15)
Note that*P<0.05，**P<0.01
The experimental result shows that compared with a blank control, the myocardial lipofuscin content of the mice in the medium-dose group is reduced to a certain extent, but no significant difference exists, and the high-dose ZYBJC has a significant effect of reducing the myocardial lipofuscin content of the aged mice and has a good effect of delaying senescence. Namely: the high-dose sports anti-fatigue traditional Chinese medicine health tea has a remarkable effect of reducing the content of myocardial lipofuscin of aged mice, and has a good effect of delaying senescence.
2. Determination of liver superoxide dismutase (SOD) Activity
After the mouse is decapitated, a proper amount of liver is taken and added into pre-cooled 0.1mol/L PH8.2Tris-HCL buffer solution to prepare 10% (w/v) tissue homogenate. Centrifuging the homogenized liquid at 1300rpm and 4 deg.C for 10min to obtain supernatant as crude enzyme solution for determination. Determination of the rate of auto-oxidation of pyrogallol: 10ul of pyrogallol solution was added to 4.5ml of Tris-HCl buffer which had been incubated at 25 ℃ for 20min, immediately started the timing, and quickly shaken up, poured into a quartz cuvette with an optical path of 1cm, and the absorbance A was measured every 30 seconds at X-325, and read 5min for each tube. The sample solutions were tested in the same manner and the results are shown in Table 5.
TABLE 5 Effect of ZYBJC on mouse superoxide dismutase activity (X + -SD, n 15)
Note that*P<0.05，**P<0.01
The experimental result shows that compared with a blank control, the SOD activity of the mice in the low-dose group has an increasing trend but no significant change, and the SOD activity of the mice in the medium-dose group and the high-dose group is obviously enhanced (P < 0.05). Therefore, the ZYBJC with medium and high doses can obviously improve the SOD activity of the aged mice and has better effect of delaying senility. Namely: the traditional Chinese medicine health tea for resisting fatigue in sports with medium and high doses can obviously improve the SOD activity of aged mice and has better effect of delaying senility. All the tests have no side effect and good effect.
Claims (4)
1. An anti-fatigue traditional Chinese medicine health tea for sports, which is characterized by comprising the following raw materials: ginseng, prepared rehmannia root, dogwood fruit, Chinese yam, asparagus root, dwarf lilyturf tuber, hawthorn fruit, tuckahoe, root bark of tree peony, oriental waterplantain rhizome, Chinese magnoliavine fruit, maltose, fried dodder seed, medlar, deerhorn glue, kudzu root, prepared fleece-flower root, paper mulberry fruit, flatstem milkvetch seed and honey-fried licorice root, wherein the ginseng accounts for 10 to 20 parts by weight, the prepared rehmannia root accounts for 5 to 15 parts by weight, the dogwood fruit accounts for 5 to 15 parts by weight, the Chinese yam accounts for 10 to 14 parts by weight, the asparagus root accounts for 5 to 15 parts by weight, the dwarf lilyturf tuber accounts for 5 to 15 parts by weight, the hawthorn fruit accounts for 5 to 15 parts by weight, the tuckahoe accounts for 5 to 15 parts by weight, the cortex moutan root accounts for 5 to 15 parts by weight, the oriental waterplantain rhizome accounts for 5 to 15 parts by weight, the schisandra fruit accounts for 5 to, 6-10 parts of fried semen cuscutae, 6-15 parts of wolfberry fruit, 5-10 parts of deerhorn glue, 6-15 parts of kudzu root, 5-10 parts of prepared fleece-flower root, 5-10 parts of paper mulberry fruit, 5-10 parts of flatstem milkvetch seed and 5-10 parts of honey-fried licorice root.
2. The sports antifatigue Chinese medicinal health tea as claimed in claim 1, wherein said ginseng is 10 parts by weight, said prepared rehmannia root is 5 parts by weight, said dogwood is 5 parts by weight, said yam is 10 parts by weight, said asparagus is 5 parts by weight, said ophiopogon root is 5 parts by weight, said hawthorn is 5 parts by weight, said poria is 5 parts by weight, said cortex moutan is 5 parts by weight, said alisma orientale is 5 parts by weight, said schisandra chinensis is 5 parts by weight, said sugar is 8 parts by weight, said parched dodder is 6 parts by weight, said medlar is 6 parts by weight, said antler glue is 5 parts by weight, said pueraria root is 6 parts by weight, said prepared fleece-flower root is 5 parts by weight, said papermulberry fruit is 5 parts by weight, said flastem milkvetch is 5 parts by weight, the honey-fried licorice root is prepared with honey and honey in 5 weight portions.
3. The sports antifatigue Chinese medicinal health tea as claimed in claim 1, wherein said ginseng is 20 parts by weight, said prepared rehmannia root is 15 parts by weight, said cornus officinalis is 15 parts by weight, said yam is 14 parts by weight, said asparagus is 15 parts by weight, said ophiopogon root is 15 parts by weight, said hawthorn is 15 parts by weight, said poria cocos is 15 parts by weight, said cortex moutan is 15 parts by weight, said alisma orientale is 15 parts by weight, said schisandra chinensis is 15 parts by weight, said sugar is 14 parts by weight, said parched dodder is 10 parts by weight, said wolfberry fruit is 15 parts by weight, said antler glue is 10 parts by weight, said pueraria root is 15 parts by weight, said prepared fleece-flower root is 10 parts by weight, said broussonetia papyrifera is 10 parts by weight, said flastem milkvetch seed is 10 parts by weight, the honey-fried licorice root is 10 parts by weight.
4. The sports antifatigue Chinese medicinal health tea as claimed in claim 1, wherein said ginseng is 15 parts by weight, said prepared rehmannia root is 10 parts by weight, said cornus officinalis is 10 parts by weight, said yam is 12 parts by weight, said asparagus is 8 parts by weight, said ophiopogon root is 8 parts by weight, said hawthorn is 10 parts by weight, said poria cocos is 10 parts by weight, said cortex moutan is 10 parts by weight, said alisma orientale is 10 parts by weight, said schisandra chinensis is 10 parts by weight, said sugar is 10 parts by weight, said parched dodder is 8 parts by weight, said wolfberry fruit is 10 parts by weight, said antler glue is 8 parts by weight, said pueraria root is 10 parts by weight, said prepared fleece-flower root is 8 parts by weight, said broussonetia papyrifera is 8 parts by weight, said flastem milkvetch is 8 parts by weight, the honey-fried licorice root is prepared in parts by weight of 6.
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