CN105890944B - Cell block preparation facilities with mechanical arm - Google Patents
Cell block preparation facilities with mechanical arm Download PDFInfo
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- CN105890944B CN105890944B CN201610204152.2A CN201610204152A CN105890944B CN 105890944 B CN105890944 B CN 105890944B CN 201610204152 A CN201610204152 A CN 201610204152A CN 105890944 B CN105890944 B CN 105890944B
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- Prior art keywords
- tube body
- conduit
- test tube
- mechanical arm
- fixing piece
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 238000012360 testing method Methods 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 47
- 239000012528 membrane Substances 0.000 claims abstract description 35
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 30
- 230000001413 cellular effect Effects 0.000 claims abstract description 9
- 239000002184 metal Substances 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 description 44
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 24
- 238000000034 method Methods 0.000 description 19
- 239000012188 paraffin wax Substances 0.000 description 18
- 230000008569 process Effects 0.000 description 12
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 10
- 230000018044 dehydration Effects 0.000 description 9
- 238000006297 dehydration reaction Methods 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000007170 pathology Effects 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 206010048612 Hydrothorax Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of cell block preparation facilities with mechanical arm, including turntable, test tube, semi-permeable membrane, liquid taking device, draw-out device and pressurizing device.Test tube is fixed in the first fixation hole on turntable, and test tube includes the first tube body and the second tube body, and the first tube body and the second tube body are detachable connection, and the bottom of the second tube body has aperture.Semi-permeable membrane is set between the first tube body and the second tube body.Liquid taking device is for drawing cellular liquid, reagent and atoleine, including mechanical arm, suction pipe, the first conduit and working barrel.Draw-out device includes fixing piece and the second conduit, and fixing piece is hollow structure, and the top opening of fixing piece is mutually fixedly connected with the aperture of the second bottom, and the bottom opening of fixing piece is mutually fixedly connected with one end of the second conduit.Pressurizing device is connected with the other end of the second conduit, provides positive pressure or negative pressure by the second conduit for test tube bottom.
Description
It is on April 23rd, 2014 that the application, which is the applying date, entitled " automatic application No. is 201410166957.3
Prepare the devices and methods therefor of cell block " divisional application.
Technical field
The present invention relates to medical electronic instrument fields, and in particular to a kind of cell block with mechanical arm prepares dress
It sets.
Background technique
Medically processing body fluid cell is usually and the cell with liquid is coated directly on sheet glass, then pass through microscope
It is observed.With the development of cell pathology in recent years, the limitation of the common smear of the above this routine techniques production is got over
It is more obvious, such as smear Existence and uniquenss, not reproducible；Cannot permanently it retain；Hinder emerging pathological technique such as immune group
Change, the application of molecular pathology technology.
Cell block is fixed paraffin is carried out after the cell extraction in liquid, and allied organization's slice is made after fixed
Form.This method solve above-mentioned common smear there are the problem of, also can avoid because of smear damage, the doctor that may cause such as lose
Suffer from dispute.Making cell block simultaneously is also the basis for preparing organization chip, which will be widely used in cell pathology
Field.
Production cell block substantially uses manual method to handle at present, the body fluid cell of processing may include hydrothorax,
Ascites, cervical exfoliated cell, dropsy of serous cavity, cerebrospinal fluid, sputum etc..Hand-made brief step is as follows: by extraction
Body fluid pours into test tube, by the test tube high speed centrifugation some time, removes test tube top and contains the supernatant of the interference components such as blood,
The liquid cell collected is set to form cell mass；Complete cell mass under careful removing, is wrapped with permeable filter paper, after solid
Embedded wax stone, is then cut by the series of steps such as fixed, dehydration, transparent, dyeing, waxdip, cooling, embedding under slicer
About 5 μm of thin slice, to be finally completed cell block.
In above step, dehydration, transparent, waxdip can be automatically performed in tissue processor, but remaining step is present
It is all by having been manually done, time-consuming is about 7~8 hours, and during which after the completion of some step, operator must manual intervention
It can be carried out in next step, it is time-consuming, laborious.And the experience level of hand-made cell block and operator are directly associated, and it is no
Then it is likely to the irregular situation of wax stone quality occur；In addition manual operations is also possible to the examination such as meeting and formalin, dimethylbenzene
Agent is in contact, careless slightly to injure to the health care belt of operator.
Summary of the invention
The present invention is in order to overcome existing cell block production need to be using having been manually done, and not only operation precision is low and easily to behaviour
The problem of body of author brings injury provides a kind of cell block preparation facilities with mechanical arm.
To achieve the goals above, the present invention provides a kind of cell block preparation facilities with mechanical arm, including turntable,
Test tube, semi-permeable membrane, liquid taking device, draw-out device and pressurizing device.Turntable has multiple first fixation holes.The quantity of test tube is extremely
It is one few, and it is fixed on the first fixation hole, test tube includes the first tube body and the second tube body, and the first tube body and the second tube body are that can divide
It is connected from formula, the bottom of the second tube body has aperture, and the second tube body is in hollow rounding mesa-shaped, and is located at the second bottom
For the area of aperture less than the area of the second bottom, the lateral wall of the first tube body is provided with stop block, the lower edge of stop block
It is in the same plane with the joint face of the first tube body and the second tube body.Semi-permeable membrane is set between the first tube body and the second tube body,
The upper surface and lower surface of semi-permeable membrane is all covered with metal mesh, and metal mesh and semi-permeable membrane are arranged at the first tube body and
Between two tube bodies.
Liquid taking device includes mechanical arm, suction pipe, the first conduit and working barrel.Wherein mechanical arm is that test tube adds cell liquid
Body, reagent and atoleine.Mechanical arm includes bottom, rotation section and clamping part, and bottom has multiple through-holes, and mechanical arm is
Hollow structure, and controller is provided in rotation section.Suction pipe is set to clamping part.Inside first conduit via through holes and mechanical arm
It is connected with suction pipe, for the circulation of liquid, solenoid valve is provided on the first conduit, first conduit is correspondingly arranged on one
Solenoid valve, and solenoid valve and controller are electrically connected.Working barrel is set to the first conduit, will be located in the reagent bottle below suction pipe
Reagent aspiration to suction pipe in, and first conduit is correspondingly arranged on a working barrel；Wherein working barrel and controller are electrical
Connection.
Draw-out device includes fixing piece and the second conduit, and fixing piece is hollow structure, the top opening of fixing piece and second
The aperture of bottom is mutually fixedly connected, and the bottom opening of fixing piece is mutually fixedly connected with one end of the second conduit, fixing piece
Top is provided with groove corresponding with the second tube body of hollow rounding mesa-shaped, and the both sides of groove are provided with working arm.Pressurization
Device is connected with the other end of the second conduit, provides positive pressure or negative pressure by the second conduit for test tube bottom.
In conclusion the cell block preparation facilities provided by the invention with mechanical arm, first in liquid taking device is led
Cellular liquid to be prepared and the reagent in reagent bottle are added to by pipe and working barrel by mechanical arm and suction pipe
In test tube.Machinery takes liquid manually to take liquid instead of traditional, and reagent is not contacted with operator, and operator's body not will receive wound
Evil.It also can reach the additive amount of control reagent by control working barrel, precision is higher compared with manual operation.It is pressurizeed by setting
Device impregnate formalin, dehydration, dyeing, it is transparent and impregnate paraffin and etc. in constantly repeat to test tube bottom increase just
Pressure or negative pressure, so that the cell being located on semi-permeable membrane is adequately reacted with added reagent.User need to only set as needed
Pressurizing device adds the time of positive pressure and negative pressure, can be automatically performed the above process.
In addition, the upper surface and lower surface in semi-permeable membrane is respectively provided with metal mesh, when heating device to paraffin pipeline and
When test tube, metal mesh has good thermally conductive function can be by the heat transfer to semi-permeable membrane on test tube, so that on semi-permeable membrane
Paraffin keeps liquid during impregnating paraffin.
For above and other objects of the present invention, feature and advantage can be clearer and more comprehensible, preferred embodiment is cited below particularly,
And cooperate attached drawing, it is described in detail below.
Detailed description of the invention
Fig. 1 show the cell block preparation facilities schematic diagram with mechanical arm of one embodiment of the invention offer；
Fig. 2 show the schematic cross-section of test tube in Fig. 1, fixing piece and pressurizing device；
Fig. 3 show the work flow diagram of the cell block preparation facilities shown in FIG. 1 with mechanical arm.
Specific embodiment
Fig. 1 show the cell block preparation facilities schematic diagram with mechanical arm of one embodiment of the invention offer；
Fig. 2 show the schematic cross-section of test tube in Fig. 1, fixing piece and pressurizing device；Fig. 3 show shown in FIG. 1
The work flow diagram of cell block preparation facilities with mechanical arm.Also referring to Fig. 1 to Fig. 3.
The present invention provides a kind of cell block preparation facilities with mechanical arm, including turntable 1, test tube 2, semi-permeable membrane 3, takes
Liquid device 4, draw-out device 5 and pressurizing device 6.Turntable 1 has multiple first fixation holes 11.The quantity of test tube 2 is at least one
It is a, and it is fixed on the first fixation hole 11.Test tube 2 includes the first tube body 21 and the second tube body 22, the first tube body 21 and the second tube body
22 be detachable connection, and the bottom of the second tube body 22 has aperture 221.Semi-permeable membrane 3 is set to the first tube body 21 and the second pipe
Between body 22.Liquid taking device 4 includes mechanical arm 41, suction pipe 42, the first conduit 43 and working barrel 44.Wherein mechanical arm 41 is test tube
2 addition reagents or atoleine.Mechanical arm 41 includes bottom 411, rotation section 412 and clamping part 413, and bottom 411 has more
A through-hole.Mechanical arm 41 is hollow structure, and is provided with controller in rotation section 412.Suction pipe 42 is set to clamping part 413.The
One conduit, 43 via through holes and 41 inside of mechanical arm are connected with suction pipe 42, the circulation for liquid.Working barrel 44 is set to
One conduit 43 will be located in the reagent aspiration to suction pipe 42 in the reagent bottle below suction pipe 42, and first conduit 43 is corresponding
It is provided with a working barrel 44.Draw-out device 5 includes fixing piece 51 and the second conduit 52, and fixing piece 51 is hollow structure, fixed
The top opening of part 51 is fixedly connected with 221 phase of aperture of 22 bottom of the second tube body, and the bottom opening of fixing piece 51 is led with second
One end of pipe 52 is mutually fixedly connected.Pressurizing device 6 is connected with the other end of the second conduit 52, is test tube by the second conduit 52
2 bottoms provide positive pressure or negative pressure.Wherein, solenoid valve 45 is additionally provided on the first conduit 43, and the first conduit 43 correspondence is set
It is equipped with a solenoid valve 45.
In this present embodiment, liquid taking device 4 has two sets, wherein a set of for extracting cellular liquid and formal to be produced
The liquid such as woods, alcohol and dimethylbenzene, and in the set liquid taking device mechanical arm 41 the tool of bottom 411 there are four through-hole, have respectively
Four the first conduits 43 pass through the corresponding through-hole and are connected with the suction pipe 42 on the liquid taking device 4, are respectively used to draw Fu Er
Malin, concentration are 95% alcohol, absolute alcohol and dimethylbenzene, and another set of liquid taking device 4 is for extracting paraffin.However, this hair
It is bright that this is not limited in any way.In other embodiments, a set of liquid taking device 4 can be used, but be arranged in the bottom of mechanical arm 41
Five or more through-holes, and two clamping parts 413 and two suction pipes 42 are set, one of suction pipe 42 is for drawing formal
The reagents such as woods and cellular liquid to be prepared, and another suction pipe 42 is for drawing paraffin.
Below with reference to Fig. 1 and Fig. 3 to the workflow of the cell block preparation facilities provided by the invention with mechanical arm
It is described in detail.
The process originates in sampling, it would be desirable to which the cellular liquid for producing cell block draws into the test tube 2 with semi-permeable membrane 3
Interior (step S101).Suction pipe is rinsed after having drawn.
It impregnates formalin (step S102).User opens the first conduit being connected with the reagent bottle for holding formalin
Working barrel 44 and solenoid valve 45 on 43, it is thin to being placed with that suction pipe 42 draws the neutral formalin that 5ml concentration is 10%
In the test tube 2 of cytosol body.
Pressurizing device 6 is opened, negative pressure is increased in test tube bottom by the second conduit 52, so that moisture, small molecule and good fortune
You pass through semi-permeable membrane 3 by Malin's liquid, and the cell in cellular liquid is then trapped on semi-permeable membrane 3.When what is accumulated on semi-permeable membrane 3
Cell is more when blocking semi-permeable membrane 3.Pressurizing device 6 is set, and reversion control pump increases positive pressure in test tube bottom, will get lodged in semi-transparent
Cell on film 3 pushes back in cellular liquid again.Increase negative pressure again again later, the liquid of mix reagent is passed through into semi-permeable membrane 3
It sucks in waste liquid bottle.It constantly repeats the above process, so that all cells on semi-permeable membrane 3 sufficiently impregnate formalin, the mistake
Journey lasts 2 hours, must assure that the formalin liquid of at least 2ml or more in test tube in the process.
In order to guarantee the formalin liquid of at least 2ml or more in test tube, in the present embodiment, by the side of test tube 2
Sensor is set on wall, and the sensor and controller are electrically connected.Liquid level in controller real-time detection test tube 2, works as examination
When liquid level in pipe 2 is lower than 2ml, controller output control signal opens working barrel 44 and solenoid valve 45, and suction pipe 42 is inhaled
It takes in formalin to test tube, realizes the process of automatic soaking formalin.
It is dehydrated (step S103), which includes primary dewatering and second dehydration, primary dewatering and second dehydration
Process is identical as the process of formalin is impregnated, and only reagent used in primary dewatering is the alcohol that concentration is 95%, dehydration
Time is 1.5 hours.And reagent used in second dehydration is absolute alcohol, and dewatering time is also 1.5 hours.
It dyes (step S104), the Yihong 0.8ml is added in test tube 2, the cell of second dehydration is dyed, constantly
It repeats to increase negative pressure, positive pressure and negative pressure to test tube bottom, so that the cell being detained on semi-permeable membrane 3 sufficiently dyes, which is gone through
When 10 minutes.
Transparent (step S105) in vitro adds dimethylbenzene and carries out transparent processing, constantly weight to the cell after dyed
It is multiple that 30 minutes are lasted to 2 bottom of test tube increase negative pressure, positive pressure and negative pressure, the process.
It impregnates paraffin (step S106), heating fills the reagent bottle, paraffin suction pipe and test tube of paraffin, by reagent bottle
Interior is paraffin melting, and the paraffin after thawing is added to test tube 2, to 2 bottom of test tube constantly repeat increase negative pressure, positive pressure and
Negative pressure, the process last 1.5 hours, dimethylbenzene remaining on cell after transparent processing are cemented out, in this process test tube 2
Temperature at 60 DEG C~70 DEG C.In the present embodiment, to prevent the paraffin cooled and solidified on semi-permeable membrane 3 in step s 106, half
The upper surface and lower surface of permeable membrane 3 is all covered with metal mesh 7, and metal mesh 7 and semi-permeable membrane 3 be arranged at the first tube body and
Between second tube body, the temperature of test tube 2 quickly can be passed to semi-permeable membrane 3 by metal mesh 7, so that the temperature of semi-permeable membrane 3 maintains
60 DEG C~70 DEG C.In addition, metal mesh 7 can also play a supporting role to semi-permeable membrane 2.
In above-mentioned steps S102~step S106, made by pressurizing device 6 cell being trapped on semi-permeable membrane 3 with
The reagent or paraffin of each step addition repeat to react, and all processes are automatically performed, and each step need to be only arranged in user
The time of time and pressurizing device 6 plus positive pressure or negative pressure.Operator will not endanger operation without contacting any reagent
The health of person.Further, by increasing solenoid valve 45, by controlling the valve opening of solenoid valve 45, it can be achieved that reagent
Accurate absorption.And the signal that increases the liquid level that sensor comes in test tubes 2, and will test passes to controller,
Controller controls the working condition of solenoid valve 45 and working barrel 44 according to the detection signal, realizes and automatically controls.
Cell block removal on semi-permeable membrane 3 is put into embedding frame after step S106 is completed and test tube 2 is cooling and is carried out
It embeds and embedded cell block is placed in slice (step S107) under slicer.
In this present embodiment, the second tube body 22 is in hollow rounding mesa-shaped, and is located at the aperture 221 of 22 bottom of the second tube body
Area less than 22 bottom of the second tube body area.The top of fixing piece 51 corresponding with the rounding mesa-shaped structure is provided with recessed
Slot 511, and the both sides of groove 511 are provided with working arm 512.Liquid can be concentrated and be flowed by the second tube body 22 of rounding mesa-shaped structure
In hollow fixing piece 51.Meanwhile the area of the aperture 221 of 22 bottom of the second tube body is less than the area of 22 bottom of the second tube body,
Any leakage will not occur for liquid.
Rounding mesa-shaped structure can preferably protect the second tube body 22, prevent the second tube body 22 chipping.It is further
The second tube body 22 is protected, the side wall of groove 511 is not only provided with bolster 513, while also in the lateral wall of the first tube body 21
It is provided with stop block 211, the lower edge of stop block 211 and the joint face of the first tube body 21 and the second tube body 22 are generally aligned in the same plane
On.When the rising of fixing piece 51 is fixedly connected with the second tube body 22, stop block 211 stops working arm 512, prevents from fixing
Part 51 excessively rises, so that the bottom of the second tube body 22 is squeezed and chipping.
In conclusion the cell block preparation facilities and its method provided by the invention with mechanical arm, in liquid taking device 4
The first conduit 43 and working barrel 44 cellular liquid to be prepared and reagent in reagent bottle passed through into mechanical arm 41
And suction pipe 42 is added in test tube 2.Machinery takes liquid manually to take liquid instead of traditional, and reagent is not contacted with operator, operation
Person's body will not come to harm.The additive amount that also can reach control reagent by control working barrel 44, the essence compared with manual operation
Du Genggao.By setting pressurizing device 6 impregnate formalin, dehydration, dyeing, it is transparent and impregnate paraffin and etc. in constantly
It repeats to increase positive pressure or negative pressure to 2 bottom of test tube, so that the cell being located on semi-permeable membrane 3 is adequately anti-with added reagent
It answers.User need to only set the time of pressurizing device 6 plus positive pressure and negative pressure as needed, can be automatically performed the above process.
In addition, metal mesh 7 is arranged in the upper surface and lower surface in semi-permeable membrane 3, when heating device to paraffin pipeline and
When test tube, metal mesh 7 has good thermally conductive function can be by the heat transfer on test tube to semi-permeable membrane 3, so that on semi-permeable membrane 3
Paraffin keep liquid during impregnating paraffin.
Although the present invention is disclosed above by preferred embodiment, however, it is not intended to limit the invention, this any known skill
Skill person can make some changes and embellishment without departing from the spirit and scope of the present invention, therefore protection scope of the present invention is worked as
Subject to claims range claimed.
Claims (1)
1. a kind of cell block preparation facilities with mechanical arm characterized by comprising
Turntable has multiple first fixation holes；
Test tube, the quantity of the test tube is at least one, and is fixed on the first fixation hole, and the test tube includes the first tube body and the
Two tube bodies, first tube body and the second tube body are detachable connection, and the bottom of second tube body has aperture, the second pipe
Body is in hollow rounding mesa-shaped, and the area positioned at the area of the aperture of the second bottom less than the second bottom, described
The lateral wall of first tube body is provided with stop block, the connection of the lower edge of the stop block and first tube body and the second tube body
Face is in the same plane；
Semi-permeable membrane is set between first tube body and the second tube body, and the upper surface and lower surface of the semi-permeable membrane covers
There is metal mesh, and the metal mesh and semi-permeable membrane are arranged between first tube body and the second tube body；
Liquid taking device adds cellular liquid, reagent and atoleine for test tube, comprising:
Mechanical arm, including bottom, rotation section and clamping part, the bottom have multiple through-holes, and the mechanical arm is hollow knot
Structure, and controller is provided in the rotation section；
Suction pipe is set to the clamping part；
First conduit, through being connected inside the through-hole and mechanical arm with the suction pipe, for the circulation of liquid, the first conduit
On be provided with solenoid valve, first conduit is correspondingly arranged on a solenoid valve, and the solenoid valve and controller are electrically connected；
Working barrel is set to first conduit, will be located at the reagent aspiration in the reagent bottle below the suction pipe to the suction
In pipe, and first conduit is correspondingly arranged on a working barrel；Wherein the working barrel and the controller are electrically connected；
Draw-out device, including fixing piece and the second conduit, the fixing piece be hollow structure, the top opening of the fixing piece with
The aperture of second bottom is mutually fixedly connected, and the bottom opening of fixing piece is mutually fixedly connected with one end of the second conduit,
The top of fixing piece is provided with groove corresponding with the second tube body of hollow rounding mesa-shaped, and the both sides of the groove are provided with
Working arm；
Pressurizing device is connected with the other end of second conduit, provides positive pressure by the second conduit for test tube bottom or bears
Pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610204152.2A CN105890944B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with mechanical arm |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610204152.2A CN105890944B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with mechanical arm |
| CN201410166957.3A CN103969096B (en) | 2014-04-23 | 2014-04-23 | Automatically device and the method thereof of cell block is prepared |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410166957.3A Division CN103969096B (en) | 2014-04-23 | 2014-04-23 | Automatically device and the method thereof of cell block is prepared |
Publications (2)
| Publication Number | Publication Date |
|---|---|
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| CN201610182572.5A Active CN105865864B (en) | 2014-04-23 | 2014-04-23 | Cell block processing unit (plant) with quick preparation function |
| CN201610186890.9A Active CN105842028B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with pressurization, extract function |
| CN201610172989.3A Expired - Fee Related CN105628477B (en) | 2014-04-23 | 2014-04-23 | The full-automatic device for quickly preparing cell block |
| CN201610254620.7A Active CN105928757B (en) | 2014-04-23 | 2014-04-23 | Self-action cell block preparation system |
| CN201610187761.1A Active CN105865888B (en) | 2014-04-23 | 2014-04-23 | The preparation system of cell block |
| CN201410166957.3A Active CN103969096B (en) | 2014-04-23 | 2014-04-23 | Automatically device and the method thereof of cell block is prepared |
| CN201610204152.2A Expired - Fee Related CN105890944B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with mechanical arm |
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| CN201610191848.6A Active CN105890943B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with automatic detection function |
| CN201610182572.5A Active CN105865864B (en) | 2014-04-23 | 2014-04-23 | Cell block processing unit (plant) with quick preparation function |
| CN201610186890.9A Active CN105842028B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with pressurization, extract function |
| CN201610172989.3A Expired - Fee Related CN105628477B (en) | 2014-04-23 | 2014-04-23 | The full-automatic device for quickly preparing cell block |
| CN201610254620.7A Active CN105928757B (en) | 2014-04-23 | 2014-04-23 | Self-action cell block preparation system |
| CN201610187761.1A Active CN105865888B (en) | 2014-04-23 | 2014-04-23 | The preparation system of cell block |
| CN201410166957.3A Active CN103969096B (en) | 2014-04-23 | 2014-04-23 | Automatically device and the method thereof of cell block is prepared |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104324668B (en) * | 2014-11-26 | 2016-08-31 | 乐卫敏 | A kind of tissue embedding machine wax melting device |
| CN106644635A (en) * | 2016-12-07 | 2017-05-10 | 贵州大学 | Automatic medicine changing device and method for paraffin sections |
| CN108760447A (en) * | 2018-05-23 | 2018-11-06 | 深圳大学 | Semi-automatic dyeing apparatus |
| CN113390700B (en) * | 2021-05-24 | 2022-12-23 | 杭州电子科技大学 | Automatic cell wax block preparation system and method based on centrifugal method |
| CN113390692B (en) * | 2021-05-24 | 2023-02-28 | 杭州电子科技大学 | Centrifugal test tube for automatically preparing cell wax block |
| CN113390699B (en) * | 2021-05-24 | 2022-12-09 | 杭州电子科技大学 | Device and method for preparing cell wax block in stamping mode |
| TWI777599B (en) * | 2021-06-04 | 2022-09-11 | 國立中興大學 | cell grabbing device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201413267Y (en) * | 2009-05-20 | 2010-02-24 | 赵晓辉 | Eight-jar automatic staining machine for hematoxylin and eosin (HE) staining of biological tissue paraffin section |
| CN101992136A (en) * | 2010-09-30 | 2011-03-30 | 何洪敏 | Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells |
| CN201841020U (en) * | 2010-10-26 | 2011-05-25 | 徐海苗 | Filtering type moulding centrifugal tube for manufacturing cell wax block |
| CN102764133A (en) * | 2012-08-10 | 2012-11-07 | 上海科华检验医学产品有限公司 | Vacuum blood collection tube and method thereof capable of directly separating blood plasma |
| CN103543057A (en) * | 2013-11-13 | 2014-01-29 | 何洪敏 | Combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1029228B1 (en) * | 1997-11-04 | 2002-03-13 | Monogen, Inc. | Method and apparatus for mixing and separating particulate matter from a liquid specimen |
| ES2372169T3 (en) * | 2002-10-31 | 2012-01-16 | University Of Massachusetts | METHOD AND APPLIANCE FOR QUICK EMBOTTING OF BLOCKS WITH CELLS. |
| CN1253716C (en) * | 2004-01-08 | 2006-04-26 | 中南大学湘雅医学院肿瘤研究所 | Tissue chip used for tumour early stage diagnosis and preparation device |
| CN100451649C (en) * | 2004-01-08 | 2009-01-14 | 中南大学湘雅医学院肿瘤研究所 | Cell chip manufacturing method and its device |
| CN101061383B (en) * | 2004-11-24 | 2012-08-22 | 李荣山 | Cytoblock preparation method |
| CN100460850C (en) * | 2005-07-20 | 2009-02-11 | 徐华 | Making method of body fluid cell lump paraffin section |
| WO2007109639A2 (en) * | 2006-03-20 | 2007-09-27 | Rongshan Li | Cytoblock preparation system and methods of use |
| US7914462B2 (en) * | 2006-08-15 | 2011-03-29 | Cytyc Corporation | Methods for processing cell block |
| CN100587452C (en) * | 2007-04-23 | 2010-02-03 | 中国农业大学 | Method for preparing oocyte paraffin cut film |
| CN101344467A (en) * | 2008-08-20 | 2009-01-14 | 赵晓辉 | Routine paraffin wax flaking HE dyeing non-xylol whole-course treatment liquid for biological organization sample |
| EP2458387A3 (en) * | 2010-11-30 | 2013-09-18 | Kabushiki Kaisha Yaskawa Denki | specimen processing system |
| WO2013059526A1 (en) * | 2011-10-18 | 2013-04-25 | The Trustees Of Columbia University In The City Of New York | Medical apparatus and method for collecting biological samples |
| CN203908836U (en) * | 2014-04-23 | 2014-10-29 | 杭州电子科技大学 | Automatic device for preparing cell wax blocks |
-
2014
- 2014-04-23 CN CN201610191848.6A patent/CN105890943B/en active Active
- 2014-04-23 CN CN201610182572.5A patent/CN105865864B/en active Active
- 2014-04-23 CN CN201610186890.9A patent/CN105842028B/en active Active
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- 2014-04-23 CN CN201610254620.7A patent/CN105928757B/en active Active
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- 2014-04-23 CN CN201410166957.3A patent/CN103969096B/en active Active
- 2014-04-23 CN CN201610204152.2A patent/CN105890944B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201413267Y (en) * | 2009-05-20 | 2010-02-24 | 赵晓辉 | Eight-jar automatic staining machine for hematoxylin and eosin (HE) staining of biological tissue paraffin section |
| CN101992136A (en) * | 2010-09-30 | 2011-03-30 | 何洪敏 | Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells |
| CN201841020U (en) * | 2010-10-26 | 2011-05-25 | 徐海苗 | Filtering type moulding centrifugal tube for manufacturing cell wax block |
| CN102764133A (en) * | 2012-08-10 | 2012-11-07 | 上海科华检验医学产品有限公司 | Vacuum blood collection tube and method thereof capable of directly separating blood plasma |
| CN103543057A (en) * | 2013-11-13 | 2014-01-29 | 何洪敏 | Combined type centrifuge tube for preparation of cytological paraffin block by using trace cast-off cells |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105865888B (en) | 2019-02-22 |
| CN105865888A (en) | 2016-08-17 |
| CN105890943A (en) | 2016-08-24 |
| CN103969096B (en) | 2016-06-01 |
| CN105890943B (en) | 2019-01-25 |
| CN105842028B (en) | 2019-02-22 |
| CN105928757A (en) | 2016-09-07 |
| CN105842028A (en) | 2016-08-10 |
| CN103969096A (en) | 2014-08-06 |
| CN105865864B (en) | 2019-01-25 |
| CN105865864A (en) | 2016-08-17 |
| CN105890944A (en) | 2016-08-24 |
| CN105628477A (en) | 2016-06-01 |
| CN105628477B (en) | 2018-01-16 |
| CN105928757B (en) | 2019-01-25 |
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