CN105865888A - Preparation system of cell paraffin blocks - Google Patents
Preparation system of cell paraffin blocks Download PDFInfo
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- CN105865888A CN105865888A CN201610187761.1A CN201610187761A CN105865888A CN 105865888 A CN105865888 A CN 105865888A CN 201610187761 A CN201610187761 A CN 201610187761A CN 105865888 A CN105865888 A CN 105865888A
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- conduit
- test tube
- liquid
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- semipermeable membrane
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000012188 paraffin wax Substances 0.000 title abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 54
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 239000012528 membrane Substances 0.000 claims abstract description 36
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 29
- 229940057995 liquid paraffin Drugs 0.000 claims abstract description 4
- 230000001413 cellular effect Effects 0.000 claims description 10
- 210000003141 lower extremity Anatomy 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000005086 pumping Methods 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 description 38
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 24
- 238000000034 method Methods 0.000 description 22
- 230000008569 process Effects 0.000 description 16
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 8
- 230000018044 dehydration Effects 0.000 description 8
- 238000006297 dehydration reaction Methods 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 230000006378 damage Effects 0.000 description 5
- 238000002791 soaking Methods 0.000 description 5
- 239000001993 wax Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 206010048612 Hydrothorax Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a preparation system of cell paraffin blocks. The preparation system comprises a rotating disk, a test tube, a semi-permeable membrane, a liquid sucking device, a pumping device and a pressurizing device. The test tube is fixedly arranged in the first fixed hole of the rotating disk, the test tube comprises a first tube body and a second tube body which are detachably connected, and the bottom of the second tube body is provided with a hole. The semi-permeable membrane is arranged between the first tube body and the second tube body. The liquid sucking device is used for sucking a cell liquid, a reagent and liquid paraffin, and comprises a mechanical arm, a suction tube, first conduits and working pumps. The pumping device comprises a fixed part and a second conduit, the fixed part is a hollow structure, the top opening of the fixed part is fixedly connected with the hole at the bottom of the second tube body, and the bottom opening of the fixed part is fixedly connected with one end of the second conduit. The pressurizing device is connected with the other end of the second conduit, and provides a positive pressure or a negative pressure for the bottom of the test tube through the second conduit.
Description
The application is filing date on April 23rd, 2014, Application No. 201410166957.3, invention name
It is referred to as the divisional application of " automatically preparing the devices and methods therefor of cell block ".
Technical field
The present invention relates to medical electronics instrument field, and particularly to the preparation system of a kind of cell block.
Background technology
Medically process body fluid cell usually the cell with liquid to be coated directly on sheet glass, then pass through
Microscope is observed.Along with the most cytopathologic development, it is general that above this routine techniques makes
The limitation of logical smear is more and more obvious, such as smear Existence and uniquenss, it is impossible to replicate；Can not forever retain；
Hinder the application that such as SABC, molecular pathology learn a skill of emerging pathological technique.
Cell block is then to fix carrying out paraffin after the cell extraction in liquid, is formed like tissue after fixing
The form of section.This method solve above-mentioned common smear exist problem, also can avoid because of smear damage,
The doctor-patient dispute that loss etc. may cause.Make cell block is also the basis preparing organization chip simultaneously, should
Technology will be widely used in cytopathology field.
Making cell block at present substantially uses manual method to process, and the body fluid cell of process can include
Hydrothorax, ascites, cervical exfoliated cell, dropsy of serous cavity, cerebrospinal fluid, sputum etc..Make by hand briefly
Step as follows: pour the body fluid of extraction into test tube, by the test tube high speed centrifugation some time, remove on test tube
Portion contains the supernatant of the interference components such as blood, makes the liquid cell of collection form cell mass；Careful stripping
From lower complete cell mass, wrap with permeable filter paper, go through fixing, dehydration, transparent, dye, soak
Wax, cool down, the series of steps such as embedding, then embedded wax stone is cut under microtome about 5 μm
Thin slice, thus it is finally completed cell block.
In above step, dehydration, transparent, waxdip can be automatically performed in tissue processor, but remaining
Step is the most all by having been manually done, and is time-consumingly about 7～8 hours, after certain step of period completes, and behaviour
Make personnel and manual intervention just must can carry out next step, time-consuming, laborious.And make cell block by hand,
It is directly associated with the experience level of operator, is otherwise likely to that the uneven situation of wax stone quality occurs；
Additionally manual operations is also possible to contact with the reagent such as formalin, dimethylbenzene, and careless slightly will giving is grasped
The health making personnel brings injury.
Summary of the invention
The present invention has been manually done to overcome existing cell block to make to use, and not only performance accuracy is low and pole
Easily give the problem that the health of operator brings injury, it is provided that the preparation system of a kind of cell block.
To achieve these goals, the present invention provides the preparation system of a kind of cell block, including rotating disk, examination
Pipe, semipermeable membrane, liquid taking device, draw-out device, pressue device and sensor.Rotating disk has multiple first
Fixing hole.The quantity of test tube is at least one, and is fixed on the first fixing hole, test tube include the first body and
Second body, the first body and the second body are detachable connection, and the bottom of the second body has perforate,
Second body is the rounding mesa-shaped of hollow, and the area being positioned at the perforate of the second its bottom is less than the second body
Bottom area, the lateral wall of the first body is provided with stop block, the lower limb of stop block and the first body and
The joint face of the second body is in the same plane.Semipermeable membrane is arranged between the first body and the second body, and half
The upper surface of permeable membrane and lower surface are all covered with wire netting, and wire netting and semipermeable membrane are arranged at first
Between body and the second body.
Liquid taking device is that test tube adds cellular liquid, reagent and liquid paraffin, it include mechanical arm, suction pipe,
First conduit, working barrel and draw-out device.Mechanical arm includes bottom, rotation section and clamping part, and bottom has
Having multiple through hole, mechanical arm is to be provided with controller in hollow structure, and rotation section.Suction pipe is arranged at clamping
Portion.First conduit via through holes and mechanical arm inside are connected with suction pipe, and for the circulation of liquid, first leads
Being provided with electromagnetic valve on pipe, first conduit is correspondingly arranged on an electromagnetic valve, and electromagnetic valve and controller
It is electrically connected with.Working barrel is arranged at the first conduit, and the reagent aspiration that will be located in the reagent bottle below suction pipe arrives
In suction pipe, and first conduit is correspondingly arranged on a working barrel；Wherein working barrel electrically connects with controller
Connect.
Draw-out device includes fixture and the second conduit, and fixture is hollow structure, the open top of fixture
Perforate with the second its bottom is fixedly connected, and the bottom opening of fixture and one end of the second conduit are solid mutually
Fixed connection, the top of fixture is provided with the groove corresponding with the second body of hollow rounding mesa-shaped, and recessed
The both sides of groove are provided with working arm, and the sidewall of groove is provided with bolster.Pressue device and the second conduit another
One end is connected, by the second conduit for providing malleation or negative pressure bottom test tube.Sensor is arranged at test tube
Sidewall, sensor is electrically connected with the controller in rotation section, the liquid level of liquid in the real-time test tubes of sensor
Highly.
In sum, the preparation system of cell block that the present invention provides, the first conduit in liquid taking device with
And working barrel by cellular liquid to be prepared and is positioned at the reagent of reagent bottle and is added by mechanical arm and suction pipe
It is added in test tube.Machinery takes liquid and instead of and traditional manually take liquid, and reagent does not contacts with operator, operator
Health will not come to harm.Also the addition of control reagent is can reach, with manual operation by controlling working barrel
Compare precision higher.By arrange pressue device soak formalin, be dehydrated, dye, transparent and leaching
The steps such as afrodite wax constantly repeat to increasing malleation or negative pressure bottom test tube so that be positioned on semipermeable membrane is thin
Born of the same parents sufficiently with the reagent reacting added.User only need to set pressue device on demand and add malleation and negative pressure
Time, said process can be automatically performed.
Additionally, upper surface and lower surface at semipermeable membrane are respectively provided with wire netting, when heater is to paraffin pipe
When road and test tube, wire netting has good heat conduction function and can be delivered on semipermeable membrane by the heat on test tube,
The paraffin on semipermeable membrane is made to keep liquid during soaking paraffin.
For above and other objects of the present invention, feature and advantage can be become apparent, cited below particularly preferably
Embodiment, and coordinate accompanying drawing, it is described in detail below.
Accompanying drawing explanation
Fig. 1 show the preparation system schematic diagram of the cell block that one embodiment of the invention provides；
Fig. 2 show the schematic cross-section of test tube in Fig. 1, fixture and pressue device；
Fig. 3 show the workflow diagram of the preparation system of the cell block shown in Fig. 1.
Detailed description of the invention
Fig. 1 show the preparation system schematic diagram of the cell block that one embodiment of the invention provides；Shown in Fig. 2
For the schematic cross-section of test tube, fixture and pressue device in Fig. 1；It is thin that Fig. 3 show shown in Fig. 1
The workflow diagram of the preparation system of born of the same parents' wax stone.See also Fig. 1 to Fig. 3.
The present invention provides the preparation system of a kind of cell block, including rotating disk 1, test tube 2, semipermeable membrane 3, takes
Liquid device 4, draw-out device 5 and pressue device 6.Rotating disk 1 has multiple first fixing hole 11.Test tube 2
Quantity be at least one, and be fixed on the first fixing hole 11.Test tube 2 includes the first body 21 and the second pipe
Body 22, the first body 21 and the second body 22 are detachable connection, and the bottom of the second body 22 has out
Hole 221.Semipermeable membrane 3 is arranged between the first body 21 and the second body 22.Liquid taking device 4 includes mechanical arm
41, suction pipe the 42, first conduit 43 and working barrel 44.Wherein mechanical arm 41 for test tube 2 add reagent or
Liquid paraffin.Mechanical arm 41 includes bottom 411, rotation section 412 and clamping part 413, and bottom 411 has
There is multiple through hole.Mechanical arm 41 is to be provided with controller 8 in hollow structure, and rotation section 412.Suction pipe 42
It is arranged at clamping part 413.First conduit 43 via through holes and mechanical arm 41 inside are connected with suction pipe 42,
Circulation for liquid.Working barrel 44 is arranged at the first conduit 43, will be located in the reagent bottle below suction pipe 42
In reagent aspiration in suction pipe 42, and first conduit 43 is correspondingly arranged on a working barrel 44.Take out
Fetching is put 5 and is included fixture 51 and the second conduit 52, and fixture 51 is hollow structure, the top of fixture 51
Perforate 221 bottom portion's opening and the second body 22 is fixedly connected, the bottom opening of fixture 51 and
One end of two conduits 52 is fixedly connected.Pressue device 6 is connected with the other end of the second conduit 52, logical
Cross the second conduit 52 for providing malleation or negative pressure bottom test tube 2.Wherein, the first conduit 43 is additionally provided with
Electromagnetic valve 45, and first conduit 43 is correspondingly arranged on an electromagnetic valve 45.
In the present embodiment, liquid taking device 4 has two sets, the most a set of for extracting cellular liquid to be produced
With the bottom 411 of mechanical arm 41 in the liquid, and this set liquid taking device such as formalin, ethanol and dimethylbenzene
There are four through holes, have four the first conduits 43 to pass on this corresponding through hole and this liquid taking device 4 respectively
Suction pipe 42 is connected, and being respectively used to draw formalin, concentration is 95% ethanol, anhydrous alcohol and diformazan
Benzene, another set of liquid taking device 4 is used for extracting paraffin.But, this is not limited in any way by the present invention.Yu Qi
In its embodiment, a set of liquid taking device 4 can be used, but more than five logical is set in the bottom of mechanical arm 41
Hole, and two clamping parts 413 and two suction pipes 42 are set, one of them suction pipe 42 is used for drawing formal
The reagent such as woods and cellular liquid to be prepared, and another suction pipe 42 is used for drawing paraffin.
Below in conjunction with Fig. 1 and Fig. 3, the workflow of the preparation system of the cell block that the present invention provides is carried out
Describe in detail.
This process originates in sampling, it would be desirable to produces the cellular liquid of cell block and draws into and have semipermeable membrane 3
Test tube 2 in (step S101).After having drawn, suction pipe is rinsed.
Soak formalin (step S102).User opens be connected with the reagent bottle holding formalin
Working barrel 44 on one conduit 43 and electromagnetic valve 45, the neutral good fortune that 5ml concentration is 10% drawn by suction pipe 42
Your Malin is in the test tube 2 being placed with cellular liquid.
Open pressue device 6, bottom test tube, increase negative pressure by the second conduit 52 so that moisture, little point
Son and formalin liquid are by semipermeable membrane 3, and the cell in cellular liquid is then trapped on semipermeable membrane 3.
When on semipermeable membrane 3 accumulation cell more block semipermeable membrane 3 time.Arranging pressue device 6, reversion controls pump,
Bottom test tube, increase malleation, the cell got lodged on semipermeable membrane 3 is pushed back in cellular liquid again.The most again
Again increase negative pressure, the liquid of mix reagent is sucked in waste liquid bottle by semipermeable membrane 3.Constantly repeat above-mentioned
Process so that all cells on semipermeable membrane 3 the most fully soaks formalin, this process lasts 2 hours,
Cross punching must assure that the formalin liquid of at least more than 2ml in test tube at this.
In order to ensure the formalin liquid of at least more than 2ml in test tube, in the present embodiment, by examination
Arranging sensor on the sidewall of pipe 2, this sensor is electrically connected with controller.The real-time test tubes of controller 2
Interior liquid level, when the liquid level in test tube 2 is less than 2ml, controller output control signal is opened
Working barrel 44 and electromagnetic valve 45, suction pipe 42 draws formalin in test tube, it is achieved automatic soaking Fu Er
The process of Malin.
Dehydration (step S103), this dehydration includes primary dewatering and second dehydration, primary dewatering and secondary
The process of dehydration is identical with the process soaking formalin, and reagent simply used in primary dewatering is that concentration is
The ethanol of 95%, dewatering time is 1.5 hours.And the reagent used by second dehydration is anhydrous alcohol, and it is dehydrated
Time is also 1.5 hours.
Dyeing (step S104), adds 0.8ml Yihong in test tube 2 and contaminates the cell of second dehydration
Color, constantly repeats increasing negative pressure, malleation and negative pressure bottom test tube so that is detained on semipermeable membrane 3 is thin
Born of the same parents fully dye, and this process lasts 10 minutes.
Transparent (step S105), in vitro adds dimethylbenzene and the cell after dyed is carried out transparent processing,
Constantly repeating increasing negative pressure, malleation and negative pressure bottom test tube 2, this process lasts 30 minutes.
Soaking paraffin (step S106), heating fills the reagent bottle of paraffin, paraffin suction pipe and test tube,
Paraffin melting by reagent bottle, the paraffin after melting adds to test tube 2, to bottom test tube 2 constantly
Repeating to increase negative pressure, malleation and negative pressure, this process lasts 1.5 hours, will remain on cell after transparent processing
Dimethylbenzene cement out, the temperature of test tube 2 is at 60 DEG C～70 DEG C in this process.In the present embodiment, it is anti-
Paraffin cooled and solidified on semipermeable membrane 3 the most in step s 106, at upper surface and the lower surface of semipermeable membrane 3
It is all covered with wire netting 7, and wire netting 7 and semipermeable membrane 3 is arranged between the first body and the second body,
Wire netting 7 can quickly by the temperature transfer of test tube 2 to semipermeable membrane 3 so that the temperature of semipermeable membrane 3 maintains
60 DEG C～70 DEG C.Additionally, semipermeable membrane 2 also can be played a supporting role by wire netting 5.
In above-mentioned steps S102～step S106, make to be trapped on semipermeable membrane 3 by pressue device 6
The reagent that adds of cell and each step or paraffin repeat reaction, all of process is all automatically performed, user
Time of each step only need to be set and pressue device 6 adds time of malleation or negative pressure.Operator without
Any reagent need to be contacted, the healthy of operator will not be endangered.Further, by increasing electromagnetic valve
45, by controlling the valve opening of electromagnetic valve 45, the accurate absorption of reagent can be realized.And increase sensor
Liquid level in test tubes 2, and the signal detected is passed to controller, controller is according to this inspection
Survey signal and control electromagnetic valve 45 and the duty of working barrel 44, it is achieved automatically control.
Embedding frame is put into when step S106 completes and removed by the cell block on semipermeable membrane 3 after test tube 2 cooling
Inside carry out embedding and embedded cell block being placed under microtome section (step S107).
In the present embodiment, the second body 22 rounding mesa-shaped in hollow, and be positioned at bottom the second body 22
The area of perforate 221 less than the area bottom the second body 22.With this rounding corresponding consolidating of mesa-shaped structure
The top of locking member 51 is provided with groove 511, and the both sides of groove 511 are provided with working arm 512.Inverted round stage
In liquid can be concentrated the fixture 51 flowing into hollow by the second body 22 of shape structure.Meanwhile, the second body
The area of the perforate 221 bottom 22 is less than the area bottom the second body 22, and liquid will not occur any seepage.
Rounding mesa-shaped structure can preferably protect the second body 22, prevents the second body 22 chipping.For entering
Protection second body 22 of one step, not only the sidewall at groove 511 is provided with bolster 513, the most also exists
The lateral wall of the first body 21 is provided with stop block 211, the lower limb of stop block 211 and the first body 21 and
The joint face of the second body 22 is in the same plane.When fixture 51 rises fixing even with the second body 22
When connecing, working arm 512 is stopped by stop block 211, prevents fixture 51 from too rising, so that
The bottom of the second body 22 is squeezed and chipping.
In sum, the preparation system of the cell block that the present invention provides and method thereof, in liquid taking device 4
First conduit 43 and working barrel 44 are by cellular liquid to be prepared and be positioned at the reagent of reagent bottle and pass through
Mechanical arm 41 and suction pipe 42 add in test tube 2.Machinery takes liquid and instead of traditional liquid that manually takes, examination
Agent does not contacts with operator, and operator's health will not come to harm.Also control is can reach by controlling working barrel 44
The addition of reagent processed, precision is higher compared with manual operation.Fu Er is being soaked by arranging pressue device 6
Malin, be dehydrated, dye, transparent and soak in the steps such as paraffin and constantly repeat just to increase to bottom test tube 2
Pressure or negative pressure so that be positioned at the reagent reacting that the cell on semipermeable membrane 3 is sufficient and is added.User only needs
Set pressue device 6 on demand and add the time of malleation and negative pressure, said process can be automatically performed.
Additionally, upper surface and lower surface at semipermeable membrane 3 arrange wire netting 7, when heater is to paraffin pipe
When road and test tube, wire netting 7 has good heat conduction function can be delivered to semipermeable membrane 3 by the heat on test tube
On so that the paraffin on semipermeable membrane 3 keeps liquid during soaking paraffin.
Although the present invention is disclosed above by preferred embodiment, but it is not limited to the present invention, any ripe
Know this those skilled in the art, without departing from the spirit and scope of the present invention, a little change and retouching can be made, therefore
Protection scope of the present invention is when being as the criterion depending on claims scope required for protection.
Claims (1)
1. the preparation system of a cell block, it is characterised in that including:
Rotating disk, has multiple first fixing hole；
Test tube, the quantity of described test tube is at least one, and is fixed on the first fixing hole, and described test tube includes
First body and the second body, described first body and the second body are detachable connection, described second pipe
The bottom of body has perforate, and the second body is the rounding mesa-shaped of hollow, and is positioned at the perforate of the second its bottom
Area less than the area of the second its bottom, the lateral wall of described first body is provided with stop block, described
The lower limb of stop block is in the same plane with the joint face of described first body and the second body；
Semipermeable membrane, is arranged between described first body and the second body, the upper surface of described semipermeable membrane and under
Surface is all covered with wire netting, and described wire netting and semipermeable membrane are arranged at described first body and second
Between body；
Liquid taking device, adds cellular liquid, reagent and liquid paraffin for test tube, including:
Mechanical arm, including bottom, rotation section and clamping part, described bottom has multiple through hole, institute
Stating mechanical arm is to be provided with controller in hollow structure, and described rotation section；
Suction pipe, is arranged at described clamping part；
First conduit, is connected with described suction pipe, for liquid through described through hole and mechanical arm inside
Circulation, the first conduit is provided with electromagnetic valve, first conduit is correspondingly arranged on an electromagnetic valve,
And described electromagnetic valve is electrically connected with controller；
Working barrel, is arranged at described first conduit, will be located in the examination in the reagent bottle below described suction pipe
Agent is drawn in described suction pipe, and first conduit is correspondingly arranged on a working barrel；Wherein said
Working barrel is electrically connected with described controller；
Draw-out device, including fixture and the second conduit, described fixture is hollow structure, described fixture
The perforate of open top and described second its bottom be fixedly connected, the bottom opening of fixture and second
One end of conduit is fixedly connected, and the top of fixture is provided with relative with the second body of hollow rounding mesa-shaped
The groove answered, and the both sides of described groove are provided with working arm, the sidewall of groove is provided with bolster；
Pressue device, is connected with the other end of described second conduit, is to carry bottom test tube by the second conduit
For malleation or negative pressure；
Sensor, is arranged at the sidewall of described test tube, and described sensor is electrically connected with the control in described rotation section
Device processed, the liquid level of liquid in the real-time test tubes of sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610187761.1A CN105865888B (en) | 2014-04-23 | 2014-04-23 | The preparation system of cell block |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610187761.1A CN105865888B (en) | 2014-04-23 | 2014-04-23 | The preparation system of cell block |
| CN201410166957.3A CN103969096B (en) | 2014-04-23 | 2014-04-23 | Automatically device and the method thereof of cell block is prepared |
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| CN201610191848.6A Active CN105890943B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with automatic detection function |
| CN201610182572.5A Active CN105865864B (en) | 2014-04-23 | 2014-04-23 | Cell block processing unit (plant) with quick preparation function |
| CN201610186890.9A Active CN105842028B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with pressurization, extract function |
| CN201610172989.3A Expired - Fee Related CN105628477B (en) | 2014-04-23 | 2014-04-23 | The full-automatic device for quickly preparing cell block |
| CN201610254620.7A Active CN105928757B (en) | 2014-04-23 | 2014-04-23 | Self-action cell block preparation system |
| CN201610187761.1A Active CN105865888B (en) | 2014-04-23 | 2014-04-23 | The preparation system of cell block |
| CN201410166957.3A Active CN103969096B (en) | 2014-04-23 | 2014-04-23 | Automatically device and the method thereof of cell block is prepared |
| CN201610204152.2A Expired - Fee Related CN105890944B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with mechanical arm |
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| CN201610191848.6A Active CN105890943B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with automatic detection function |
| CN201610182572.5A Active CN105865864B (en) | 2014-04-23 | 2014-04-23 | Cell block processing unit (plant) with quick preparation function |
| CN201610186890.9A Active CN105842028B (en) | 2014-04-23 | 2014-04-23 | Cell block preparation facilities with pressurization, extract function |
| CN201610172989.3A Expired - Fee Related CN105628477B (en) | 2014-04-23 | 2014-04-23 | The full-automatic device for quickly preparing cell block |
| CN201610254620.7A Active CN105928757B (en) | 2014-04-23 | 2014-04-23 | Self-action cell block preparation system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104324668B (en) * | 2014-11-26 | 2016-08-31 | 乐卫敏 | A kind of tissue embedding machine wax melting device |
| CN106644635A (en) * | 2016-12-07 | 2017-05-10 | 贵州大学 | Automatic medicine changing device and method for paraffin sections |
| CN108760447A (en) * | 2018-05-23 | 2018-11-06 | 深圳大学 | Semi-automatic dyeing apparatus |
| CN113390700B (en) * | 2021-05-24 | 2022-12-23 | 杭州电子科技大学 | Automatic cell wax block preparation system and method based on centrifugal method |
| CN113390692B (en) * | 2021-05-24 | 2023-02-28 | 杭州电子科技大学 | Centrifugal test tube for automatically preparing cell wax block |
| CN113390699B (en) * | 2021-05-24 | 2022-12-09 | 杭州电子科技大学 | Device and method for preparing cell wax block in stamping mode |
| TWI777599B (en) * | 2021-06-04 | 2022-09-11 | 國立中興大學 | cell grabbing device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004041994A1 (en) * | 2002-10-31 | 2004-05-21 | University Of Massachusetts | Rapid cell block embedding method and apparatus |
| CN101144760A (en) * | 2007-04-23 | 2008-03-19 | 中国农业大学 | Method for preparing oocyte paraffin cut film |
| CN101344467A (en) * | 2008-08-20 | 2009-01-14 | 赵晓辉 | Routine paraffin wax flaking HE dyeing non-xylol whole-course treatment liquid for biological organization sample |
| EP2623950A1 (en) * | 2006-08-15 | 2013-08-07 | Cytyc Corporation | Cell block embedding system and methods |
| JP2015501424A (en) * | 2011-10-18 | 2015-01-15 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | Medical device and method for collecting biological samples |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1029228B1 (en) * | 1997-11-04 | 2002-03-13 | Monogen, Inc. | Method and apparatus for mixing and separating particulate matter from a liquid specimen |
| CN1253716C (en) * | 2004-01-08 | 2006-04-26 | 中南大学湘雅医学院肿瘤研究所 | Tissue chip used for tumour early stage diagnosis and preparation device |
| CN100451649C (en) * | 2004-01-08 | 2009-01-14 | 中南大学湘雅医学院肿瘤研究所 | Cell chip manufacturing method and its device |
| CN101061383B (en) * | 2004-11-24 | 2012-08-22 | 李荣山 | Cytoblock preparation method |
| CN100460850C (en) * | 2005-07-20 | 2009-02-11 | 徐华 | Making method of body fluid cell lump paraffin section |
| WO2007109639A2 (en) * | 2006-03-20 | 2007-09-27 | Rongshan Li | Cytoblock preparation system and methods of use |
| CN201413267Y (en) * | 2009-05-20 | 2010-02-24 | 赵晓辉 | Eight-jar automatic staining machine for hematoxylin and eosin (HE) staining of biological tissue paraffin section |
| CN101992136B (en) * | 2010-09-30 | 2012-09-05 | 何洪敏 | Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells |
| CN201841020U (en) * | 2010-10-26 | 2011-05-25 | 徐海苗 | Filtering type moulding centrifugal tube for manufacturing cell wax block |
| EP2458387A3 (en) * | 2010-11-30 | 2013-09-18 | Kabushiki Kaisha Yaskawa Denki | specimen processing system |
| CN102764133A (en) * | 2012-08-10 | 2012-11-07 | 上海科华检验医学产品有限公司 | Vacuum blood collection tube and method thereof capable of directly separating blood plasma |
| CN103543057B (en) * | 2013-11-13 | 2015-12-02 | 何鑫 | The Combined centrifuge tube of cytology wax stone is prepared for micro-cast-off cells |
| CN203908836U (en) * | 2014-04-23 | 2014-10-29 | 杭州电子科技大学 | Automatic device for preparing cell wax blocks |
-
2014
- 2014-04-23 CN CN201610191848.6A patent/CN105890943B/en active Active
- 2014-04-23 CN CN201610182572.5A patent/CN105865864B/en active Active
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004041994A1 (en) * | 2002-10-31 | 2004-05-21 | University Of Massachusetts | Rapid cell block embedding method and apparatus |
| EP2623950A1 (en) * | 2006-08-15 | 2013-08-07 | Cytyc Corporation | Cell block embedding system and methods |
| CN101144760A (en) * | 2007-04-23 | 2008-03-19 | 中国农业大学 | Method for preparing oocyte paraffin cut film |
| CN101344467A (en) * | 2008-08-20 | 2009-01-14 | 赵晓辉 | Routine paraffin wax flaking HE dyeing non-xylol whole-course treatment liquid for biological organization sample |
| JP2015501424A (en) * | 2011-10-18 | 2015-01-15 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | Medical device and method for collecting biological samples |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105865888B (en) | 2019-02-22 |
| CN105890943A (en) | 2016-08-24 |
| CN103969096B (en) | 2016-06-01 |
| CN105890943B (en) | 2019-01-25 |
| CN105842028B (en) | 2019-02-22 |
| CN105928757A (en) | 2016-09-07 |
| CN105842028A (en) | 2016-08-10 |
| CN103969096A (en) | 2014-08-06 |
| CN105890944B (en) | 2019-01-25 |
| CN105865864B (en) | 2019-01-25 |
| CN105865864A (en) | 2016-08-17 |
| CN105890944A (en) | 2016-08-24 |
| CN105628477A (en) | 2016-06-01 |
| CN105628477B (en) | 2018-01-16 |
| CN105928757B (en) | 2019-01-25 |
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