CN105859899A - Rhizoma gastrodiae polysaccharide extraction method - Google Patents
Rhizoma gastrodiae polysaccharide extraction method Download PDFInfo
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- CN105859899A CN105859899A CN201610231405.5A CN201610231405A CN105859899A CN 105859899 A CN105859899 A CN 105859899A CN 201610231405 A CN201610231405 A CN 201610231405A CN 105859899 A CN105859899 A CN 105859899A
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- gastrodia elata
- recovery rate
- extracting
- rhizoma gastrodiae
- elata polysaccharide
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The invention discloses a rhizoma gastrodiae polysaccharide extraction method. The method includes the steps that extraction process conditions are determined; rhizoma gastrodiae polysaccharide is extracted in an ultrasonic-assisted mode according to the extraction process conditions. According to the extraction process conditions, ultrasonic wave output power is larger than 0%, extraction temperature is 40-80 DEG C, extraction time ranges from 15 min to 40 min, and the material to solution ratio is 1:(10-50) g/mL. The extraction rate of rhizoma gastrodiae polysaccharide can be increased.
Description
Technical field
The present invention relates to medical domain, a kind of method extracted in particular to gastrodia elata polysaccharide.
Background technology
Rhizoma Gastrodiae have another name called rhizoma gastrodiae, solely shake sesame, close from grass, Bai Longpi etc., be the orchid family Gastrodia herbaceos perennial.Generation
In boundary, only China, Korea, Japan and area, the former Soviet Union Far East produce rhizoma Gastrodiae, but yield is most with China, mainly
It is distributed in the ground such as Yunnan, Sichuan, Shaanxi, Hubei, Henan, Jilin, Tibet.Its rhizome is used as medicine to treat dizziness
The diseases such as dizzy, extremity numbness, child convulsion, are rare Chinese herbal medicines[12].Gastrodia elata polysaccharide has immunocompetence, exempts from human body
The regulation of epidemic disease function, the Clinics and Practices of cancer, anti-aging and release the aspect such as organism fatigue and have important effect,
Therefore people have become the popular research field of biological medicine circle to the research of gastrodia elata polysaccharide.
But, existing extraction process is relatively low to the recovery rate of gastrodia elata polysaccharide, in order to obtain enough gastrodia elata polysaccharides, needs
Consume substantial amounts of rhizoma Gastrodiae, cause the waste of rhizoma Gastrodiae resource.
For above-mentioned problem, effective solution is the most not yet proposed.
Summary of the invention
Embodiments provide a kind of method that gastrodia elata polysaccharide extracts, to improve the recovery rate of gastrodia elata polysaccharide.
An aspect according to embodiments of the present invention, it is provided that a kind of method that gastrodia elata polysaccharide extracts, comprises determining that and carries
Taking technique condition；Ultrasonic assistant is utilized to extract gastrodia elata polysaccharide according to described extracting factor；Wherein, described extraction
Process conditions include: ultrasonic power output is more than 0%, and Extracting temperature 40 DEG C to 80 DEG C, extraction time 15min is extremely
40min, solid-liquid ratio 1: 10-50g/mL.
In embodiments of the present invention, extracting factor is determined；Ultrasonic assistant is utilized according to described extracting factor
Extract gastrodia elata polysaccharide；Wherein, described extracting factor includes: ultrasonic power output is more than 0%, Extracting temperature 40 DEG C
To 80 DEG C, extraction time 15min to 40min, solid-liquid ratio 1: 10-50g/mL.So, by said extracted technique
Condition, and combine ultrasonic wave and carry out the extraction of gastrodia elata polysaccharide, reach the technique effect of the recovery rate improving gastrodia elata polysaccharide.
Accompanying drawing explanation
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes the part of the application, this
Bright schematic description and description is used for explaining the present invention, is not intended that inappropriate limitation of the present invention.At accompanying drawing
In:
Fig. 1 is the schematic flow sheet of a kind of optional gastrodia elata polysaccharide extracting method according to embodiments of the present invention；
Fig. 2 is the relation signal of a kind of optional ultrasonic power according to embodiments of the present invention and gastrodia elata polysaccharide recovery rate
Figure；
Fig. 3 is the relation schematic diagram of a kind of optional Extracting temperature according to embodiments of the present invention and gastrodia elata polysaccharide recovery rate；
Fig. 4 is the relation schematic diagram of a kind of optional extraction time according to embodiments of the present invention and gastrodia elata polysaccharide recovery rate；
Fig. 5 is the relation schematic diagram of a kind of optional solid-liquid ratio according to embodiments of the present invention and gastrodia elata polysaccharide recovery rate.
Detailed description of the invention
In order to make those skilled in the art be more fully understood that the present invention program, below in conjunction with in the embodiment of the present invention
Accompanying drawing, is clearly and completely described the technical scheme in the embodiment of the present invention, it is clear that described embodiment
It is only the embodiment of a present invention part rather than whole embodiments.Based on the embodiment in the present invention, ability
The every other embodiment that territory those of ordinary skill is obtained under not making creative work premise, all should belong to
The scope of protection of the invention.
It should be noted that term " first " in description and claims of this specification and above-mentioned accompanying drawing, "
Two " it is etc. for distinguishing similar object, without being used for describing specific order or precedence.Should be appreciated that this
Sample use data can exchange in the appropriate case, in order to embodiments of the invention described herein can with except
Here the order beyond those illustrating or describing is implemented.Additionally, term " includes " and " having " and they
Any deformation, it is intended that cover non-exclusive comprising, such as, contain series of steps or the process of unit, side
Method, system, product or equipment are not necessarily limited to those steps or the unit clearly listed, but can include the clearest
List or for intrinsic other step of these processes, method, product or equipment or unit.
Fig. 1 is the extracting method of a kind of gastrodia elata polysaccharide according to embodiments of the present invention, as it is shown in figure 1, the method includes
Following steps:
S101, determine extracting factor.
Wherein, this extracting factor includes: ultrasonic power output be more than 0%, Extracting temperature 40 DEG C to 80 DEG C,
Extraction time 15min to 40min, solid-liquid ratio 1: 10-50g/mL.
Wherein, above-mentioned ultrasonic power output can be 0%, 60%, 70%, 80%, 90%, 100%, at the present embodiment
In be 100%.
Selection to ultrasonic power below illustrates:
Solid-liquid ratio be 1: 40g/mL, extraction time be 30min, Extracting temperature be 60 DEG C, ultrasonic power output
Extract gastrodia elata polysaccharide under the conditions of being respectively 0%, 60%, 70%, 80%, 90%, 100%, measure gastrodia elata polysaccharide recovery rate,
Determine ultrasonic power.
Wherein,
In formula: C is for presetting glucose quality concentration (mg/L)；V is constant volume (mL)；M is sample dry weight (g)
As in figure 2 it is shown, the corresponding relation figure of the recovery rate that Fig. 2 is different ultrasonic power and gastrodia elata polysaccharide, by Fig. 2
It can be seen that along with the increase of ultrasonic power, recovery rate gradually rises.Ultrasonic wave can improve rhizoma Gastrodiae by a relatively large margin
The recovery rate of polysaccharide, when ultrasonic power output is 100%, recovery rate is maximum, ultrasonic wave the most in the present embodiment
Power output is 100%.
Said extracted temperature can be 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, is 40 DEG C in the present embodiment.
Selection to Extracting temperature below illustrates:
Solid-liquid ratio be 1: 40g/mL, extraction time be 30min, ultrasonic power output be 100%, Extracting temperature
Be respectively 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, extract gastrodia elata polysaccharide under the conditions of 80 DEG C, measure gastrodia elata polysaccharide recovery rate,
Determine Extracting temperature.
Wherein, the calculating of recovery rate can use above-mentioned formula (1)
As it is shown on figure 3, the corresponding relation figure of the recovery rate that Fig. 3 is different Extracting temperature and gastrodia elata polysaccharide, can by Fig. 3
To find out, along with the increase of Extracting temperature, recovery rate is gradually increased, and when Extracting temperature reaches 60 DEG C, is further added by carrying
Taking temperature, recovery rate slowly declines.Therefore, Extracting temperature is 60 DEG C in the present embodiment.
The said extracted time can be 15min, 20min, 25min, 30min, 35min, 40min, at the present embodiment
In be 30min.
Selection to extraction time below illustrates:
Solid-liquid ratio be 1: 40g/mL, Extracting temperature be 60 DEG C, ultrasonic power output be 100%, extraction time
Extract gastrodia elata polysaccharide under the conditions of being respectively 15min, 20min, 25min, 30min, 35min, 40min, measure rhizoma Gastrodiae
Polysaccharide extract rate.Determine extraction time.
Wherein, the calculating of recovery rate can use above-mentioned formula (1)
As shown in Figure 4, Fig. 4 is the corresponding relation figure of the recovery rate of different extraction time and gastrodia elata polysaccharide, can by Fig. 4
To find out, along with the increase recovery rate of extraction time is gradually increased, upon extracting between when reaching 30min, carry in increase
Taking the time, recovery rate increases inconspicuous.It is thought of as shortening extraction time, minimizing energy consumption, therefore, in the present embodiment,
Extraction time is 30min.
Above-mentioned solid-liquid ratio can be 1: 10g/mL, 1: 20g/mL, 1: 30g/mL, 1: 40g/mL, 1: 50g/mL,
In the present embodiment, this solid-liquid ratio is 1: 40g/mL.
Selection to solid-liquid ratio below illustrates:
Extracting temperature be 60 DEG C, extraction time be 30min, ultrasonic power output be 100%, solid-liquid ratio is respectively
Gastrodia elata polysaccharide is extracted under the conditions of 1: 10g/mL, 1: 20g/mL, 1: 30g/mL, 1: 40g/mL, 1: 50g/mL,
Measure gastrodia elata polysaccharide recovery rate, determine solid-liquid ratio.
Wherein, the calculating of recovery rate can use above-mentioned formula (1)
As it is shown in figure 5, Fig. 5 is the different feed liquid corresponding relation figure than the recovery rate with gastrodia elata polysaccharide, permissible by Fig. 5
Find out, recovery rate when recovery rate is slightly above 1: 50g/mL when solid-liquid ratio is 1: 40g/mL, but apparently higher than solid-liquid ratio
It it is recovery rate when 1: 10g/mL, 1: 20g/mL and 1: 30g/mL.Therefore, in the present embodiment, solid-liquid ratio is
1∶40g/mL。
S102, utilize according to this extracting factor ultrasonic assistant extract gastrodia elata polysaccharide.
So, by said extracted process conditions, and combine ultrasonic wave and carry out the extraction of gastrodia elata polysaccharide, reached to improve
The technique effect of the recovery rate of gastrodia elata polysaccharide.
The invention described above embodiment sequence number, just to describing, does not represent the quality of embodiment.
In the above embodiment of the present invention, the description to each embodiment all emphasizes particularly on different fields, and does not has in certain embodiment
The part described in detail, may refer to the associated description of other embodiments.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For Yuan, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improve and profit
Decorations also should be regarded as protection scope of the present invention.
Claims (5)
1. the extracting method of a gastrodia elata polysaccharide, it is characterised in that including:
Determine extracting factor；
Ultrasonic assistant is utilized to extract gastrodia elata polysaccharide according to described extracting factor；
Wherein, described extracting factor includes: ultrasonic power output is more than 0%, and Extracting temperature 40 DEG C is extremely
80 DEG C, extraction time 15min to 40min, solid-liquid ratio 1: 10-50g/mL.
Method the most according to claim 1, it is characterised in that described ultrasonic power output is 100%.
Method the most according to claim 1, it is characterised in that described Extracting temperature is 60 DEG C.
Method the most according to claim 1, it is characterised in that described extraction time is 30min.
Method the most according to claim 1, it is characterised in that described solid-liquid ratio is 1: 40g/mL.
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Cited By (3)
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| CN106361593A (en) * | 2016-09-22 | 2017-02-01 | 防城港市港口区思达电子科技有限公司 | Natural anti-senescence component and preparation method thereof |
| CN107759710A (en) * | 2017-09-19 | 2018-03-06 | 广东中烟工业有限责任公司 | The preparation method of largeleaf poacynum leaf extract and its application in terms of tobacco |
| CN108659141A (en) * | 2018-06-08 | 2018-10-16 | 铜仁学院 | The extracting method of its anesthesia stem polysaccharide and application |
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| CN1907432A (en) * | 2006-08-16 | 2007-02-07 | 安徽大学 | Application of polysaccharide from the Gastrodia elata Blume in health caring stuff and medicine |
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103665178A (en) * | 2013-11-19 | 2014-03-26 | 刘祥义 | Method for extracting gastrodia elata polysaccharide |
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2016
- 2016-04-10 CN CN201610231405.5A patent/CN105859899A/en active Pending
Patent Citations (3)
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| CN1907432A (en) * | 2006-08-16 | 2007-02-07 | 安徽大学 | Application of polysaccharide from the Gastrodia elata Blume in health caring stuff and medicine |
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103665178A (en) * | 2013-11-19 | 2014-03-26 | 刘祥义 | Method for extracting gastrodia elata polysaccharide |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106361593A (en) * | 2016-09-22 | 2017-02-01 | 防城港市港口区思达电子科技有限公司 | Natural anti-senescence component and preparation method thereof |
| CN107759710A (en) * | 2017-09-19 | 2018-03-06 | 广东中烟工业有限责任公司 | The preparation method of largeleaf poacynum leaf extract and its application in terms of tobacco |
| CN107759710B (en) * | 2017-09-19 | 2020-05-29 | 广东中烟工业有限责任公司 | Preparation method of ambary hemp extract and application of ambary hemp extract in tobacco |
| CN108659141A (en) * | 2018-06-08 | 2018-10-16 | 铜仁学院 | The extracting method of its anesthesia stem polysaccharide and application |
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