CN103610917A - Comprehensive extraction method of rhizoma polygonati total saponins and polygahatous polysaccharides - Google Patents
Comprehensive extraction method of rhizoma polygonati total saponins and polygahatous polysaccharides Download PDFInfo
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Abstract
The invention discloses a comprehensive extraction method of rhizoma polygonati total saponins and polygahatous polysaccharides. The comprehensive extraction method is characterized by comprising an extraction method of rhizoma polygonati total saponins and an extraction method of polygahatous polysaccharides, wherein the extraction of the rhizoma polygonati total saponins comprises the processes of extracting, degreasing and extracting; the extraction of the polygahatous polysaccharides comprises the processes of water extraction, alcohol precipitation and drying. By adopting the comprehensive extraction method, a by-product which is obtained by extraction of the rhizoma polygonati total saponins at an early stage is used for extracting the polygahatous polysaccharides, so that the medicinal material resource of the rhizoma polygonati is saved, and the utilization rate of the rhizoma polygonati resource is improved. The extraction of the polygahatous polysaccharides at a later stage is not affected by the extraction of the rhizoma polygonati total saponins at the early stage. The rhizoma polygonati total saponins extracted by the method can be used for preparing a rhizoma polygonati wine; the polygahatous polysaccharides can be used for preparing rhizoma polygonati soft capsules, rhizoma polygonati lozenges, rhizoma polygonati instant tea or rhizoma polygonati soft capsule health food.
Description
Technical field
The active component that the invention belongs to natural drug extracts field, belongs to especially the combined extraction method of a kind of saponins of rhizoma polygonati and polygonatum polysaccharide.
Background technology
Rhizoma Polygonati is the general name of Liliaceae Polygonatum plant, has another name called Rhizoma Polygonati Cirrhifolii, Caput Gallus domesticus ginseng.The total Si Shi Yu Zhong， of Polygonatum plant China has more than 30 to plant.According to the difference of former plant and Medicinal Materials Characters, Rhizoma Polygonati can be divided into three kinds of RHIZOMA POLYGONATI ZINGIBERIFORME, polygonatum sibiricum Redoute and large Rhizoma Polygonatis, contains more polysaccharide and steroidal saponin in Rhizoma Polygonati.There are some researches show that polygonatum polysaccharide has the functions such as the immunity of enhancing, antitumor, defying age, antiviral, blood fat reducing, raising memory; Saponins of rhizoma polygonati has immunity, blood sugar lowering, the antitumor of raising, improves the functions such as learning and memory.Therefore polygonatum polysaccharide and saponins of rhizoma polygonati have very high economic worth.
The saponins of rhizoma polygonati of report and polygonatum polysaccharide extraction are independent extraction mostly at present, have not made full use of Rhizoma Polygonati herb resource.The saponins of rhizoma polygonati of report extracts main ethanol extraction or the decocting of adopting and extracts, extracting solution through organic solvent extraction or after macroporous resin adsorption alcoholic solution eluting, obtain saponins of rhizoma polygonati; Polygonatum polysaccharide mainly obtains by the method for water extract-alcohol precipitation.And about the comprehensive report extracting of saponins of rhizoma polygonati and polygonatum polysaccharide seldom.China Patent No.: CN101837091A discloses < < technique > > who extracts continuously Rhizoma Polygonati Saponin and polygonatum polysaccharide, the method is passed through ethanol ultrasonic extraction, from concentrated solution, obtain saponins of rhizoma polygonati, from Rhizoma Polygonati medicinal residues, obtain polygonatum polysaccharide, saponins of rhizoma polygonati and polygonatum polysaccharide purity that the method obtains through multiple operation are higher, but complex procedures, running cost is high, is unfavorable for realizing suitability for industrialized production.
Summary of the invention
Object of the present invention aims to provide a kind of Rhizoma Polygonati resource and simple comprehensive method of extracting saponins of rhizoma polygonati and polygonatum polysaccharide of operation efficiently utilized.
The object of the invention is to adopt following technical scheme to realize:
The combined extraction method of saponins of rhizoma polygonati of the present invention and polygonatum polysaccharide, its feature is: comprise the extracting method of saponins of rhizoma polygonati and the extracting method of polygonatum polysaccharide;
The extracting method of described saponins of rhizoma polygonati is to carry out as follows:
In a, the ethanol that is 75-85% by Solomonseal Rhizome in volumetric concentration, soak 4-5 hour, water bath with thermostatic control backflow 1-2 hour under 50-60 ℃ of condition then, sucking filtration, obtains Rhizoma Polygonati extracting solution and Rhizoma Polygonati medicinal residues; The volume ratio of the ethanol that the quality of described Solomonseal Rhizome and described volumetric concentration are 75-85% is 1g:10-20ml;
B, the Rhizoma Polygonati extracting solution that step a is obtained carry out concentrating under reduced pressure and obtain concentrated solution; By the petroleum ether extraction defat of described concentrated solution; Concentrated solution after defat is obtained to butanol extraction liquid with n-butanol extraction; Described butanol extraction liquid is carried out to concentrating under reduced pressure, obtain saponins of rhizoma polygonati;
The extracting method of described polygonatum polysaccharide is to carry out as follows:
C, the Rhizoma Polygonati medicinal residues that step a is obtained volatilize ethanol and obtain without ethanol Rhizoma Polygonati medicinal residues, described, add distilled water, water bath with thermostatic control backflow 1.5-2.5 hour under 80-85 ℃ of condition, sucking filtration in without ethanol Rhizoma Polygonati medicinal residues, obtain Rhizoma Polygonati medicinal residues B and supernatant, collect supernatant; To described supernatant concentrating under reduced pressure, obtain water extract concentrated solution; The described weight ratio 1:10-15 without ethanol Rhizoma Polygonati medicinal residues and described distilled water;
D, the water extract concentrated solution of step c gained is added to volumetric concentration is that 95% ethanol carries out precipitate with ethanol, then sucking filtration after standing 24-36 hour, obtain precipitation and filtrate, in sucking filtration process, with absolute ethanol washing, precipitate, get precipitation and volatilize after ethanol-50 ℃ of vacuum lyophilizations to constant weight, obtain polygonatum polysaccharide; The volume ratio of the ethanol that described water extract concentrated solution and described volumetric concentration are 95% is 1:3-5.
The combined extraction method of saponins of rhizoma polygonati of the present invention and polygonatum polysaccharide, its feature is also: the Rhizoma Polygonati medicinal residues that step a is obtained are to soak 4-5 hour in 75-85% ethanol in volumetric concentration, then water bath with thermostatic control backflow 1-2 hour under 50-60 ℃ of condition, sucking filtration, obtain Rhizoma Polygonati extracting solution B, described Rhizoma Polygonati extracting solution B is incorporated in step a gained Rhizoma Polygonati extracting solution and carries out step b; The volume ratio of the quality of described Rhizoma Polygonati medicinal residues and described volumetric concentration 75-85% ethanol is 1g:10-20ml.
To adding distilled water in gained Rhizoma Polygonati medicinal residues B after step c sucking filtration, water bath with thermostatic control reflux, extract, 1.5-2.5 hour under 80-85 ℃ of condition, sucking filtration, collects supernatant B, and described supernatant B is incorporated in step c gained supernatant and carries out concentrating under reduced pressure; The weight ratio of described Rhizoma Polygonati medicinal residues B and described distilled water is 1:10-15.
Gained filtrate after steps d sucking filtration is concentrated to obtain to concentrated solution B, at described concentrated solution B, adding volumetric concentration is that 95% ethanol carries out precipitate with ethanol, then sucking filtration after standing 24-36 hour, obtain precipitate B and liquor B, in sucking filtration process, use absolute ethanol washing precipitate B, described precipitate B is incorporated in steps d gained precipitation.
Compared with the prior art, beneficial effect of the present invention is embodied in:
1, the combined extraction method of saponins of rhizoma polygonati of the present invention and polygonatum polysaccharide, utilize extract saponins of rhizoma polygonati gained by-product for extracting polygonatum polysaccharide early stage, save the utilization rate of Rhizoma Polygonati herb resource, raising Rhizoma Polygonati resource, and the extraction of saponins of rhizoma polygonati in earlier stage does not affect the extraction of later stage polygonatum polysaccharide;
2, the organic solvent toxicity that the inventive method is used is little, is easy to reclaim; And this method has, and operation is few, equipment is simple, easy to operate, feasibility good, low production cost, be easy to realize the features such as suitability for industrialized production.
The specific embodiment
Below in conjunction with example, the present invention is elaborated.
Embodiment 1
The combined extraction method of the present embodiment saponins of rhizoma polygonati and polygonatum polysaccharide comprises the extracting method of saponins of rhizoma polygonati and the extracting method of polygonatum polysaccharide;
1, carry out first as follows the extraction of saponins of rhizoma polygonati:
In a, the ethanol that is 75% by Solomonseal Rhizome in volumetric concentration, soak 4 hours, then under 50 ℃ of conditions, water bath with thermostatic control refluxes 2 hours, and sucking filtration, obtains Rhizoma Polygonati extracting solution and Rhizoma Polygonati medicinal residues; Rhizoma Polygonati medicinal residues are carried out to ethanol extraction for the second time according to same method, and the Rhizoma Polygonati extracting solution that merging extracted twice obtains carries out step b; The volume ratio of the ethanol that wherein quality of Solomonseal Rhizome and volumetric concentration are 75% is 1g:10ml;
B, the Rhizoma Polygonati extracting solution that step a is obtained carry out concentrating under reduced pressure and obtain concentrated solution; By concentrated solution petroleum ether extraction defat; Concentrated solution after defat is obtained to butanol extraction liquid with n-butanol extraction; Butanol extraction liquid is carried out to concentrating under reduced pressure, obtain saponins of rhizoma polygonati;
2, then carry out as follows the extraction of polygonatum polysaccharide:
C, the Rhizoma Polygonati medicinal residues that step a is obtained volatilize ethanol and obtain without ethanol Rhizoma Polygonati medicinal residues, according to the weight ratio 1:10 without ethanol Rhizoma Polygonati medicinal residues and distilled water, add distilled water, under 85 ℃ of conditions, water bath with thermostatic control refluxes 1.5 hours, sucking filtration, obtain Rhizoma Polygonati medicinal residues B and supernatant, collect supernatant; Rhizoma Polygonati medicinal residues B is extracted for the second time according to same method, merge the supernatant that extracted twice obtains; To supernatant concentrating under reduced pressure, obtain water extract concentrated solution;
D, the water extract concentrated solution of step c gained is added to volumetric concentration is that 95% ethanol carries out precipitate with ethanol, and then sucking filtration after standing 24 hours, by absolute ethanol washing precipitation, to remove wherein moisture, obtains precipitation and filtrate in sucking filtration process; The volume ratio of the ethanol that wherein water extract concentrated solution and volumetric concentration are 95% is 1:5; After filtrate is concentrated, by same method, carry out precipitate with ethanol for the second time, after sucking filtration, merge the precipitation of twice acquisition.
Get precipitation and volatilize after ethanol-50 ℃ of vacuum lyophilizations to constant weight, obtain polygonatum polysaccharide.
The extraction ratio of the resulting saponins of rhizoma polygonati of the present embodiment is 6.80%, and in saponins of rhizoma polygonati, saponin content is 28.60%; The extraction ratio of polygonatum polysaccharide is 5.58%, and polygonatum polysaccharide purity is 83.55%.
Embodiment 2
The present embodiment saponins of rhizoma polygonati and polygonatum polysaccharide combined extraction method comprise the extracting method of saponins of rhizoma polygonati and the extracting method of polygonatum polysaccharide;
1, carry out first as follows the extraction of saponins of rhizoma polygonati:
In a, the ethanol that is 85% by Solomonseal Rhizome in volumetric concentration, soak 4.5 hours, then under 60 ℃ of conditions, water bath with thermostatic control refluxes 1 hour, and sucking filtration, obtains Rhizoma Polygonati extracting solution and Rhizoma Polygonati medicinal residues; The volume ratio of the ethanol that wherein quality of Solomonseal Rhizome and volumetric concentration are 85% is 1g:20ml; Rhizoma Polygonati medicinal residues are carried out to ethanol extraction for the second time according to same method, and the Rhizoma Polygonati extracting solution that merging extracted twice obtains carries out step b;
B, the Rhizoma Polygonati extracting solution that step a is obtained carry out concentrating under reduced pressure and obtain concentrated solution; By concentrated solution petroleum ether extraction defat; Concentrated solution after defat is obtained to butanol extraction liquid with n-butanol extraction; Butanol extraction liquid is carried out to concentrating under reduced pressure, obtain saponins of rhizoma polygonati;
2, then carry out as follows the extraction of polygonatum polysaccharide:
C, the Rhizoma Polygonati medicinal residues that step a is obtained volatilize ethanol and obtain without ethanol Rhizoma Polygonati medicinal residues, according to the weight ratio 1:15 without ethanol Rhizoma Polygonati medicinal residues and distilled water, add distilled water, under 80 ℃ of conditions, water bath with thermostatic control refluxes 2.5 hours, sucking filtration, obtain Rhizoma Polygonati medicinal residues B and supernatant, collect supernatant; Rhizoma Polygonati medicinal residues B is extracted for the second time according to same method, merge the supernatant that extracted twice obtains; To supernatant concentrating under reduced pressure, obtain water extract concentrated solution;
D, the water extract concentrated solution of step c gained is added to volumetric concentration is that 95% ethanol carries out precipitate with ethanol, and then sucking filtration after standing 36 hours, by absolute ethanol washing precipitation, to remove wherein moisture, obtains precipitation and filtrate in sucking filtration process; The volume ratio of the ethanol that wherein water extract concentrated solution and volumetric concentration are 95% is 1:3; After filtrate is concentrated, by same method, carry out precipitate with ethanol for the second time, after sucking filtration, merge the precipitation of twice acquisition.
Get precipitation and volatilize after ethanol-50 ℃ of vacuum lyophilizations to constant weight, obtain polygonatum polysaccharide.
The extraction ratio of the resulting saponins of rhizoma polygonati of the present embodiment is 6.52%, and in saponins of rhizoma polygonati, saponin content is 28.23%; The extraction ratio of polygonatum polysaccharide is 5.66%, and polygonatum polysaccharide purity is 84.56%.
Embodiment 3
The present embodiment saponins of rhizoma polygonati and polygonatum polysaccharide combined extraction method comprise the extracting method of saponins of rhizoma polygonati and the extracting method of polygonatum polysaccharide;
1, carry out first as follows the extraction of saponins of rhizoma polygonati:
In a, the ethanol that is 80% by Solomonseal Rhizome in volumetric concentration, soak 5 hours, then under 55 ℃ of conditions, water bath with thermostatic control refluxes 1.5 hours, and sucking filtration, obtains Rhizoma Polygonati extracting solution and Rhizoma Polygonati medicinal residues; Rhizoma Polygonati medicinal residues are carried out to ethanol extraction for the second time according to same method, and the Rhizoma Polygonati extracting solution that merging extracted twice obtains carries out step b; The volume ratio of the ethanol that wherein quality of Solomonseal Rhizome and volumetric concentration are 80% is 1g:15ml;
B, the Rhizoma Polygonati extracting solution that step a is obtained carry out concentrating under reduced pressure and obtain concentrated solution; By concentrated solution petroleum ether extraction defat; Concentrated solution after defat is obtained to butanol extraction liquid with n-butanol extraction; Butanol extraction liquid is carried out to concentrating under reduced pressure, obtain saponins of rhizoma polygonati;
2, then carry out as follows the extraction of polygonatum polysaccharide:
C, the Rhizoma Polygonati medicinal residues that step a is obtained volatilize ethanol and obtain without ethanol Rhizoma Polygonati medicinal residues, according to the weight ratio 1:12 without ethanol Rhizoma Polygonati medicinal residues and distilled water, add distilled water, under 83 ℃ of conditions, water bath with thermostatic control refluxes 2 hours, sucking filtration, obtain Rhizoma Polygonati medicinal residues B and supernatant, collect supernatant; Rhizoma Polygonati medicinal residues B is extracted for the second time according to same method, merge the supernatant that extracted twice obtains; To supernatant concentrating under reduced pressure, obtain water extract concentrated solution;
D, the water extract concentrated solution of step c gained is added to volumetric concentration is that 95% ethanol carries out precipitate with ethanol, and then sucking filtration after standing 30 hours, by absolute ethanol washing precipitation, to remove wherein moisture, obtains precipitation and filtrate in sucking filtration process; The volume ratio of the ethanol that wherein water extract concentrated solution and volumetric concentration are 95% is 1:4; After filtrate is concentrated, by same method, carry out precipitate with ethanol for the second time, after sucking filtration, merge the precipitation of twice acquisition.
Get precipitation and volatilize after ethanol-50 ℃ of vacuum lyophilizations to constant weight, obtain polygonatum polysaccharide.
The extraction ratio of the resulting saponins of rhizoma polygonati of the present embodiment is 6.43%, and in saponins of rhizoma polygonati, saponin content is 27.65%; The extraction ratio of polygonatum polysaccharide is 5.42%, and polygonatum polysaccharide purity is 84.75%.
In embodiment 1,2,3, the saponins of rhizoma polygonati of gained can be used for preparing sealwort wine.In embodiment 1,2,3, the polygonatum polysaccharide of gained can be used for preparing Rhizoma Polygonati soft capsule, Rhizoma Polygonati buccal tablet, Rhizoma Polygonati instant tea or Rhizoma Polygonati capsule health food containing.
Claims (4)
1. a combined extraction method for saponins of rhizoma polygonati and polygonatum polysaccharide, is characterized in that: comprise the extracting method of saponins of rhizoma polygonati and the extracting method of polygonatum polysaccharide;
The extracting method of described saponins of rhizoma polygonati is to carry out as follows:
In a, the ethanol that is 75-85% by Solomonseal Rhizome in volumetric concentration, soak 4-5 hour, water bath with thermostatic control backflow 1-2 hour under 50-60 ℃ of condition then, sucking filtration, obtains Rhizoma Polygonati extracting solution and Rhizoma Polygonati medicinal residues; The volume ratio of the ethanol that the quality of described Solomonseal Rhizome and described volumetric concentration are 75-85% is 1g:10-20ml;
B, the Rhizoma Polygonati extracting solution that step a is obtained carry out concentrating under reduced pressure and obtain concentrated solution; By the petroleum ether extraction defat of described concentrated solution; Concentrated solution after defat is obtained to butanol extraction liquid with n-butanol extraction; Described butanol extraction liquid is carried out to concentrating under reduced pressure, obtain saponins of rhizoma polygonati;
The extracting method of described polygonatum polysaccharide is to carry out as follows:
C, the Rhizoma Polygonati medicinal residues that step a is obtained volatilize ethanol and obtain without ethanol Rhizoma Polygonati medicinal residues, described, add distilled water, water bath with thermostatic control backflow 1.5-2.5 hour under 80-85 ℃ of condition, sucking filtration in without ethanol Rhizoma Polygonati medicinal residues, obtain Rhizoma Polygonati medicinal residues B and supernatant, collect supernatant; To described supernatant concentrating under reduced pressure, obtain water extract concentrated solution; The described weight ratio without ethanol Rhizoma Polygonati medicinal residues and described distilled water is 1:10-15;
D, the water extract concentrated solution of step c gained is added to volumetric concentration is that 95% ethanol carries out precipitate with ethanol, then sucking filtration after standing 24-36 hour, obtain precipitation and filtrate, in sucking filtration process, with absolute ethanol washing, precipitate, get precipitation and volatilize after ethanol-50 ℃ of vacuum lyophilizations to constant weight, obtain polygonatum polysaccharide; The volume ratio of the ethanol that described water extract concentrated solution and described volumetric concentration are 95% is 1:3-5.
2. the combined extraction method of saponins of rhizoma polygonati according to claim 1 and polygonatum polysaccharide, it is characterized in that: the Rhizoma Polygonati medicinal residues that step a is obtained are to soak 4-5 hour in 75-85% ethanol in volumetric concentration, then water bath with thermostatic control backflow 1-2 hour under 50-60 ℃ of condition, sucking filtration, obtain Rhizoma Polygonati extracting solution B, described Rhizoma Polygonati extracting solution B is incorporated in step a gained Rhizoma Polygonati extracting solution and carries out step b; The volume ratio of the quality of described Rhizoma Polygonati medicinal residues and described volumetric concentration 75-85% ethanol is 1g:10-20ml.
3. the combined extraction method of saponins of rhizoma polygonati according to claim 1 and polygonatum polysaccharide, it is characterized in that: to adding distilled water in gained Rhizoma Polygonati medicinal residues B after step c sucking filtration, water bath with thermostatic control reflux, extract, 1.5-2.5 hour under 80-85 ℃ of condition, sucking filtration, collect supernatant B, described supernatant B is incorporated in step c gained supernatant and carries out concentrating under reduced pressure; The weight ratio of described Rhizoma Polygonati medicinal residues B and described distilled water is 1:10-15.
4. the combined extraction method of saponins of rhizoma polygonati according to claim 1 and polygonatum polysaccharide, it is characterized in that: gained filtrate after steps d sucking filtration is concentrated to obtain to concentrated solution B, at described concentrated solution B, adding volumetric concentration is that 95% ethanol carries out precipitate with ethanol, then sucking filtration after standing 24-36 hour, obtain precipitate B and liquor B, in sucking filtration process, use absolute ethanol washing precipitate B, described precipitate B is incorporated in steps d gained precipitation.
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Cited By (9)
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| CN104324228A (en) * | 2014-11-26 | 2015-02-04 | 安徽省旌德博仕达农业科技有限公司 | Rhizoma polygonati buccal tablet and preparation method thereof |
| CN104940609A (en) * | 2015-07-08 | 2015-09-30 | 广州白云山汉方现代药业有限公司 | Method for extracting and separating multiple active constituents from polygonatum |
| CN104983753A (en) * | 2015-06-11 | 2015-10-21 | 烟台新时代健康产业有限公司 | Extracting technology and measuring method of pine pollen total saponins |
| CN106620243A (en) * | 2015-10-30 | 2017-05-10 | 湖南神农林下中药开发有限公司 | Extraction and separation process for rhizoma polygonati |
| CN106937698A (en) * | 2017-05-08 | 2017-07-11 | 宁波检验检疫科学技术研究院 | The extracting method and extraction element of a kind of Rhizoma Polygonati extract and with antiviral and help the animal feed of growth |
| CN109320624A (en) * | 2018-09-17 | 2019-02-12 | 田金举 | A kind of extraction process of Rhizoma Polygonati extract |
| CN110527000A (en) * | 2019-09-12 | 2019-12-03 | 安徽中医药大学 | A kind of method that polygonatum cyrtonema aerial part extracts polysaccharide |
| CN113817079A (en) * | 2021-11-11 | 2021-12-21 | 云南恩润生物科技发展有限公司 | Extraction method of polygonatum polysaccharide |
| CN113912747A (en) * | 2021-09-30 | 2022-01-11 | 卢接清 | Process for continuously extracting polygonatum saponin and polygonatum polysaccharide |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104324228A (en) * | 2014-11-26 | 2015-02-04 | 安徽省旌德博仕达农业科技有限公司 | Rhizoma polygonati buccal tablet and preparation method thereof |
| CN104324228B (en) * | 2014-11-26 | 2017-09-29 | 安徽省旌德博仕达农业科技有限公司 | A kind of sealwort lozenge and preparation method thereof |
| CN104983753A (en) * | 2015-06-11 | 2015-10-21 | 烟台新时代健康产业有限公司 | Extracting technology and measuring method of pine pollen total saponins |
| CN104940609A (en) * | 2015-07-08 | 2015-09-30 | 广州白云山汉方现代药业有限公司 | Method for extracting and separating multiple active constituents from polygonatum |
| CN106620243A (en) * | 2015-10-30 | 2017-05-10 | 湖南神农林下中药开发有限公司 | Extraction and separation process for rhizoma polygonati |
| CN106937698A (en) * | 2017-05-08 | 2017-07-11 | 宁波检验检疫科学技术研究院 | The extracting method and extraction element of a kind of Rhizoma Polygonati extract and with antiviral and help the animal feed of growth |
| CN106937698B (en) * | 2017-05-08 | 2020-07-31 | 宁波检验检疫科学技术研究院 | Method and device for extracting rhizoma Polygonati extract, and animal feed with antivirus and growth promoting effects |
| CN109320624A (en) * | 2018-09-17 | 2019-02-12 | 田金举 | A kind of extraction process of Rhizoma Polygonati extract |
| CN110527000A (en) * | 2019-09-12 | 2019-12-03 | 安徽中医药大学 | A kind of method that polygonatum cyrtonema aerial part extracts polysaccharide |
| CN113912747A (en) * | 2021-09-30 | 2022-01-11 | 卢接清 | Process for continuously extracting polygonatum saponin and polygonatum polysaccharide |
| CN113912747B (en) * | 2021-09-30 | 2023-01-24 | 贵州恒定天然生物技术有限公司 | Process for continuously extracting polygonatum saponin and polygonatum polysaccharide |
| CN113817079A (en) * | 2021-11-11 | 2021-12-21 | 云南恩润生物科技发展有限公司 | Extraction method of polygonatum polysaccharide |
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