CN105837699B - 一种植物乳杆菌胞外多糖及其用于拮抗蜡样芽孢杆菌肠毒素毒性的用途 - Google Patents
一种植物乳杆菌胞外多糖及其用于拮抗蜡样芽孢杆菌肠毒素毒性的用途 Download PDFInfo
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- CN105837699B CN105837699B CN201610202595.8A CN201610202595A CN105837699B CN 105837699 B CN105837699 B CN 105837699B CN 201610202595 A CN201610202595 A CN 201610202595A CN 105837699 B CN105837699 B CN 105837699B
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- lactobacillus plantarum
- bacillus cereus
- exocellular polysaccharide
- enterotoxin
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- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 71
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 71
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 71
- 150000004676 glycans Chemical class 0.000 title claims abstract description 66
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 66
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 66
- 230000001988 toxicity Effects 0.000 title claims abstract description 12
- 231100000419 toxicity Toxicity 0.000 title claims abstract description 12
- 101001057129 Bacillus cereus Enterotoxin Proteins 0.000 title abstract description 22
- 101001057130 Bacillus cereus Enterotoxin Proteins 0.000 title abstract description 22
- 230000008485 antagonism Effects 0.000 title abstract description 6
- 210000002490 intestinal epithelial cell Anatomy 0.000 claims abstract description 19
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- 235000009508 confectionery Nutrition 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 23
- 238000000926 separation method Methods 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 21
- 238000000502 dialysis Methods 0.000 claims description 20
- 235000019441 ethanol Nutrition 0.000 claims description 19
- 241000193755 Bacillus cereus Species 0.000 claims description 18
- 101710146739 Enterotoxin Proteins 0.000 claims description 18
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- 239000012530 fluid Substances 0.000 claims description 15
- 238000001556 precipitation Methods 0.000 claims description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 230000007062 hydrolysis Effects 0.000 claims description 11
- 238000006460 hydrolysis reaction Methods 0.000 claims description 11
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 7
- 101710163270 Nuclease Proteins 0.000 claims description 7
- 239000006166 lysate Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- OUDSBRTVNLOZBN-UHFFFAOYSA-N tolazamide Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NC(=O)NN1CCCCCC1 OUDSBRTVNLOZBN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 230000002949 hemolytic effect Effects 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 2
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- 230000003833 cell viability Effects 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 239000003053 toxin Substances 0.000 description 7
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- 238000012360 testing method Methods 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 210000000813 small intestine Anatomy 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 241000257169 Bacillus cereus ATCC 10987 Species 0.000 description 3
- 241000301512 Bacillus cereus ATCC 14579 Species 0.000 description 3
- 229920002444 Exopolysaccharide Polymers 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
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- 101710112752 Cytotoxin Proteins 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
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- 238000007796 conventional method Methods 0.000 description 2
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- 239000002619 cytotoxin Substances 0.000 description 2
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- 230000000968 intestinal effect Effects 0.000 description 2
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- 235000013406 prebiotics Nutrition 0.000 description 2
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- 102000004190 Enzymes Human genes 0.000 description 1
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- 241000726221 Gemma Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
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- 238000003916 acid precipitation Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001775 anti-pathogenic effect Effects 0.000 description 1
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- 235000013361 beverage Nutrition 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
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- 239000003349 gelling agent Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
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- 239000004615 ingredient Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
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- 238000012113 quantitative test Methods 0.000 description 1
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- -1 saccharide compound Chemical class 0.000 description 1
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- 210000001519 tissue Anatomy 0.000 description 1
- 210000004916 vomit Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (3)
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CN201610202595.8A CN105837699B (zh) | 2016-04-05 | 2016-04-05 | 一种植物乳杆菌胞外多糖及其用于拮抗蜡样芽孢杆菌肠毒素毒性的用途 |
Applications Claiming Priority (1)
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CN201610202595.8A CN105837699B (zh) | 2016-04-05 | 2016-04-05 | 一种植物乳杆菌胞外多糖及其用于拮抗蜡样芽孢杆菌肠毒素毒性的用途 |
Publications (2)
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CN105837699A CN105837699A (zh) | 2016-08-10 |
CN105837699B true CN105837699B (zh) | 2018-06-22 |
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CN201610202595.8A Active CN105837699B (zh) | 2016-04-05 | 2016-04-05 | 一种植物乳杆菌胞外多糖及其用于拮抗蜡样芽孢杆菌肠毒素毒性的用途 |
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107243645B (zh) * | 2017-06-06 | 2019-12-10 | 东南大学 | 一种利用植物乳杆菌胞外多糖合成贵金属纳米颗粒的方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105255752A (zh) * | 2015-04-27 | 2016-01-20 | 南昌大学 | 高耐酸植物乳杆菌zdy2013 |
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2016
- 2016-04-05 CN CN201610202595.8A patent/CN105837699B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105255752A (zh) * | 2015-04-27 | 2016-01-20 | 南昌大学 | 高耐酸植物乳杆菌zdy2013 |
Non-Patent Citations (3)
Title |
---|
Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315;Shengjie Li et al.;《Journal of Dairy Science》;20141003;第97卷(第12期);摘要,EPS的提取、纯化、检测部分,抗菌活性部分、图6 * |
Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics;Milica Nikolic et al.;《International Journal of Food Microbiology》;20120720;第158卷;2.2节 * |
In vitro probiotic characteristics of Lactobacillus plantarum ZDY 2013 and its modulatory effect on gut microbiota of mice;Renhui Huang et al.;《Journal of Dairy Science》;20150702;第98卷(第9期);摘要,胞外多糖产量部分,表3 * |
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Effective date of registration: 20240516 Address after: 750200, 300 meters northeast of the intersection of Changtai Road and Hongyun East Road in Hongguang Town, Helan Industrial Park, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Patentee after: Ningxia Wodidi Water and Fertilizer Technology Co.,Ltd. Country or region after: China Address before: 200120 building C, No. 888, Huanhu West 2nd Road, Lingang New Area, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Patentee before: Shanghai ruishenglian Information Technology Co.,Ltd. Country or region before: China Effective date of registration: 20240514 Address after: 200120 building C, No. 888, Huanhu West 2nd Road, Lingang New Area, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Patentee after: Shanghai ruishenglian Information Technology Co.,Ltd. Country or region after: China Address before: 999 No. 330031 Jiangxi province Nanchang Honggutan University Avenue Patentee before: Nanchang University Country or region before: China |