CN105837699A - Exopolysaccharide of Lactobacillus plantarum, and application of same in antagonism against toxicity of Bacillus cereus enterotoxins - Google Patents
Exopolysaccharide of Lactobacillus plantarum, and application of same in antagonism against toxicity of Bacillus cereus enterotoxins Download PDFInfo
- Publication number
- CN105837699A CN105837699A CN201610202595.8A CN201610202595A CN105837699A CN 105837699 A CN105837699 A CN 105837699A CN 201610202595 A CN201610202595 A CN 201610202595A CN 105837699 A CN105837699 A CN 105837699A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- bacillus cereus
- exopolysaccharide
- extracellular polysaccharide
- enterotoxin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 75
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 75
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 75
- 239000000147 enterotoxin Substances 0.000 title claims abstract description 17
- 231100000655 enterotoxin Toxicity 0.000 title claims abstract description 17
- 230000001988 toxicity Effects 0.000 title claims abstract description 11
- 231100000419 toxicity Toxicity 0.000 title claims abstract description 11
- 241000193755 Bacillus cereus Species 0.000 title abstract description 19
- 229920002444 Exopolysaccharide Polymers 0.000 title abstract description 12
- 230000008485 antagonism Effects 0.000 title abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 6
- 231100000572 poisoning Toxicity 0.000 claims abstract description 5
- 230000000607 poisoning effect Effects 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract 5
- 150000004676 glycans Chemical class 0.000 claims description 88
- 229920001282 polysaccharide Polymers 0.000 claims description 88
- 239000005017 polysaccharide Substances 0.000 claims description 88
- 239000006228 supernatant Substances 0.000 claims description 46
- 239000007788 liquid Substances 0.000 claims description 37
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 35
- 101001057129 Bacillus cereus Enterotoxin Proteins 0.000 claims description 28
- 101001057130 Bacillus cereus Enterotoxin Proteins 0.000 claims description 28
- 238000000855 fermentation Methods 0.000 claims description 22
- 238000000926 separation method Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 19
- 238000000502 dialysis Methods 0.000 claims description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 14
- 101710146739 Enterotoxin Proteins 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 230000007062 hydrolysis Effects 0.000 claims description 11
- 238000006460 hydrolysis reaction Methods 0.000 claims description 11
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 239000003053 toxin Substances 0.000 claims description 8
- 231100000765 toxin Toxicity 0.000 claims description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 7
- 101710163270 Nuclease Proteins 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 7
- 239000002244 precipitate Substances 0.000 claims description 7
- 238000001556 precipitation Methods 0.000 claims description 7
- OUDSBRTVNLOZBN-UHFFFAOYSA-N tolazamide Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NC(=O)NN1CCCCCC1 OUDSBRTVNLOZBN-UHFFFAOYSA-N 0.000 claims description 7
- 230000002949 hemolytic effect Effects 0.000 claims description 3
- 210000001842 enterocyte Anatomy 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 239000005557 antagonist Substances 0.000 abstract description 2
- 230000000717 retained effect Effects 0.000 abstract description 2
- 230000001225 therapeutic effect Effects 0.000 abstract description 2
- 210000002919 epithelial cell Anatomy 0.000 abstract 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 210000000813 small intestine Anatomy 0.000 abstract 1
- 210000002490 intestinal epithelial cell Anatomy 0.000 description 21
- 239000000243 solution Substances 0.000 description 13
- 230000003833 cell viability Effects 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 11
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 239000006166 lysate Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000033228 biological regulation Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 230000012447 hatching Effects 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 241000257169 Bacillus cereus ATCC 10987 Species 0.000 description 3
- 241000301512 Bacillus cereus ATCC 14579 Species 0.000 description 3
- 208000005374 Poisoning Diseases 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000007541 cellular toxicity Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 101710112752 Cytotoxin Proteins 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 238000013196 antibiotherapy Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 231100000599 cytotoxic agent Toxicity 0.000 description 2
- 239000002619 cytotoxin Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
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- 230000000968 intestinal effect Effects 0.000 description 2
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- 230000000813 microbial effect Effects 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 235000013406 prebiotics Nutrition 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
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- 230000008359 toxicosis Effects 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
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- 235000012970 cakes Nutrition 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
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- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
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- 238000012545 processing Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- -1 saccharide compound Chemical class 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000004916 vomit Anatomy 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610202595.8A CN105837699B (en) | 2016-04-05 | 2016-04-05 | A kind of lactobacillus plantarum exocellular polysaccharide and its purposes for antagonism bacillus cereus enterotoxin toxicity |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610202595.8A CN105837699B (en) | 2016-04-05 | 2016-04-05 | A kind of lactobacillus plantarum exocellular polysaccharide and its purposes for antagonism bacillus cereus enterotoxin toxicity |
Publications (2)
Publication Number | Publication Date |
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CN105837699A true CN105837699A (en) | 2016-08-10 |
CN105837699B CN105837699B (en) | 2018-06-22 |
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CN201610202595.8A Active CN105837699B (en) | 2016-04-05 | 2016-04-05 | A kind of lactobacillus plantarum exocellular polysaccharide and its purposes for antagonism bacillus cereus enterotoxin toxicity |
Country Status (1)
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107243645A (en) * | 2017-06-06 | 2017-10-13 | 东南大学 | A kind of method of utilization Lactobacillus plantarum transposon mutagenesis noble metal nano particles |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105255752A (en) * | 2015-04-27 | 2016-01-20 | 南昌大学 | Lactobacillus plantarum ZDY2013 with high acid resistance |
-
2016
- 2016-04-05 CN CN201610202595.8A patent/CN105837699B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105255752A (en) * | 2015-04-27 | 2016-01-20 | 南昌大学 | Lactobacillus plantarum ZDY2013 with high acid resistance |
Non-Patent Citations (3)
Title |
---|
MILICA NIKOLIC ET AL.: "Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics", 《INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY》 * |
RENHUI HUANG ET AL.: "In vitro probiotic characteristics of Lactobacillus plantarum ZDY 2013 and its modulatory effect on gut microbiota of mice", 《JOURNAL OF DAIRY SCIENCE》 * |
SHENGJIE LI ET AL.: "Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315", 《JOURNAL OF DAIRY SCIENCE》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107243645A (en) * | 2017-06-06 | 2017-10-13 | 东南大学 | A kind of method of utilization Lactobacillus plantarum transposon mutagenesis noble metal nano particles |
CN107243645B (en) * | 2017-06-06 | 2019-12-10 | 东南大学 | Method for synthesizing precious metal nanoparticles by using lactobacillus plantarum exopolysaccharides |
Also Published As
Publication number | Publication date |
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CN105837699B (en) | 2018-06-22 |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
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Effective date of registration: 20240516 Address after: 750200, 300 meters northeast of the intersection of Changtai Road and Hongyun East Road in Hongguang Town, Helan Industrial Park, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Patentee after: Ningxia Wodidi Water and Fertilizer Technology Co.,Ltd. Country or region after: China Address before: 200120 building C, No. 888, Huanhu West 2nd Road, Lingang New Area, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Patentee before: Shanghai ruishenglian Information Technology Co.,Ltd. Country or region before: China Effective date of registration: 20240514 Address after: 200120 building C, No. 888, Huanhu West 2nd Road, Lingang New Area, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Patentee after: Shanghai ruishenglian Information Technology Co.,Ltd. Country or region after: China Address before: 999 No. 330031 Jiangxi province Nanchang Honggutan University Avenue Patentee before: Nanchang University Country or region before: China |