CN105823727A - Microfluid control detecting device and system - Google Patents
Microfluid control detecting device and system Download PDFInfo
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- CN105823727A CN105823727A CN201610294161.5A CN201610294161A CN105823727A CN 105823727 A CN105823727 A CN 105823727A CN 201610294161 A CN201610294161 A CN 201610294161A CN 105823727 A CN105823727 A CN 105823727A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention discloses a microfluid control detecting device which comprises a base, a sample adding device and a negative pressure pump. The sample adding device is located above the base. A chip groove and a waste liquid groove are formed in the base. The sample adding device comprises a rotating disc, an air cylinder and liquid storing pipes. The rotating disc is fixedly connected with the air cylinder. The rotating disc is provided with a plurality of fixing positions. In the using process, the liquid storing pipes are placed into the fixing positions. In the detecting process, a microfluid control chip to be detected is placed into the chip groove, the height of a rotating disc is adjusted by means of the air cylinder, and the rotating disc is controlled to rotate so that the liquid storing pipes are located above a liquid injection opening of the microfluid control chip. A liquid outlet of the microfluid control chip is connected with the negative pressure pump. The negative pressure pump is used for injecting waste liquid into the waste liquid groove. The invention further discloses a microfluid control detecting system. By means of the device and the system, fast and accurate microfluid control detection can be achieved.
Description
Technical field
The present invention relates to microfluidic chip technology, be specifically related to a kind of micro-fluidic detection device and system.
Background technology
Microflow control technique is the technology that new development in recent years is got up, and this technology, based on analytical chemistry, manipulates fluid in micron scale construction, carries out reaction detection, have that volume is little, reagent and amount of samples is few, can be with advantages such as Multi-example multi objective detections simultaneously.The detection in the past needing sample size to be several milliliters, after using microfluidic chip technology, it is only necessary to the sample size of several microlitres, it is greatly saved the consumption of sample and reagent, and performance indications are better than common detection method, it is with a wide range of applications and important using value.
Micro-fluidic chip is the main platform that microflow control technique realizes, and is primarily characterized in that by controlling fluid flowing in microchannel, completes the various functions of chip system.At present, in micro-fluidic chip system, the driving of microfluid is with to control be by integrating pump (or external pump) in the chips in a variety of manners with various forms of valve bodies with the use of realizing, the most current microfluidic analysis detecting instrument is in manual automanual state mostly, and instrument price is expensive, bulky, inconvenient operation.Making a general survey of micro-fluidic detection field, existing micro-fluidic detection device certain structures is simple, and fluid controls function singleness, although some can realize the various forms of control of microfluid, but structure is complex, processing difficulties, cost intensive, is unfavorable for producing in enormous quantities.And, existing micro-fluidic detection device need to be equipped with various bulky microfluidic control facility because the valve body function in chip is undesirable mostly, this does not obviously meet the miniaturization of microfluidic chip technology, portability requirement, it is impossible to meets micro-fluidic chip and processes the demand of complex fluid.
Summary of the invention
In view of this, the present invention proposes a kind of micro-fluidic detection device and system, can realize efficient quick micro-fluidic detection.
On the one hand, the present invention provides a kind of micro-fluidic detection device, including: base, sample adding device and negative pressure pump, sample adding device is positioned at above base, wherein, base has chip slot and waste liquid tank；Sample adding device includes rotating circular disk, cylinder and liquid storage pipe, wherein rotating circular disk and the fixing connection of cylinder；There is multiple fixed bit, during liquid storage pipe is positioned over fixed bit during use on rotating circular disk；During detection, micro-fluidic chip to be detected is placed in chip slot, height by cylinder regulation rotating circular disk, and control rotating circular disk rotation, so that liquid storage pipe is positioned at the top of the liquid injection port of micro-fluidic chip, the liquid outlet of micro-fluidic chip is connected with negative pressure pump, and negative pressure pump is for injecting waste liquid in waste liquid tank.
Preferably, the tip of described liquid storage pipe is placed with piezoelectric ceramics, for being controlled the flowing of liquid storage liquid in pipe by piezoelectric effect.
Preferably, described rotating circular disk connects Zero-point positioning system, is controlled rotation and the location of described rotating circular disk by Zero-point positioning system.
Preferably, the size of described chip slot adapts with the size of micro-fluidic chip to be detected.
Preferably, described micro-fluidic chip to be detected is the micro-fluidic chip in S bend pipe road.
On the other hand, the present invention also provides for a kind of micro-fluidic detecting system, including data acquisition module, data analysis module and micro-fluidic detection device as above；Wherein,
Data acquisition module is acquisition testing signal during detecting at described micro-fluidic detection device；
Data analysis module carries out predetermined analyzing and processing for the detection signal collecting data acquisition module.
Preferably, described data acquisition module is CCD camera.
Preferably, described data analysis module is computer.
The micro-fluidic structure of the detecting device that the embodiment of the present invention is provided is simple, easy to process, with low cost, easily realize industrialization produces.Relative to conventional detection technique, the micro-fluidic detection device of the embodiment of the present invention and system simple operation, possess the plurality of advantages such as miniaturization, automatization, portability, the most quickly detection.
Accompanying drawing explanation
Fig. 1 is the structural representation of the micro-fluidic detection device of the preferred embodiment of the present invention.
Fig. 2 is the structural representation of the base of the embodiment of the present invention.
Fig. 3 is the structural representation of the sample adding device of the embodiment of the present invention.
Fig. 4 is the structural representation of the micro-fluidic chip to be detected of the embodiment of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing and specific embodiment, technical scheme is described in detail.
The micro-fluidic detection device of the embodiment of the present invention includes: base, sample adding device and negative pressure pump, and sample adding device is positioned at above base, wherein, base has chip slot and waste liquid tank；Sample adding device includes rotating circular disk, cylinder and liquid storage pipe, wherein rotating circular disk and the fixing connection of cylinder；There is multiple fixed bit, during liquid storage pipe is positioned over fixed bit during use on rotating circular disk.During detection, micro-fluidic chip to be detected is placed in chip slot, height by cylinder regulation rotating circular disk, and control rotating circular disk rotation, so that liquid storage pipe is positioned at the top of the liquid injection port of micro-fluidic chip, the liquid outlet of micro-fluidic chip is connected with negative pressure pump, and negative pressure pump is for injecting waste liquid in waste liquid tank.
When practical operation, micro-fluidic chip to be detected is placed in the chip slot of base, connect negative pressure pump, according to detection program it needs to be determined that the quantity of liquid storage pipe, corresponding test solution is joined in liquid storage pipe, liquid storage pipe is fixed on a rotary disc, utilize cylinder to control rotating circular disk to move up and down, and control rotating circular disk rotational positioning hand or with motor, thus by the liquid injection port of the outlet alignment micro-fluidic chip of liquid storage pipe, then control test solution and flow out in injection micro-fluidic chip, negative pressure pump is started after question response, waste liquid is injected in waste liquid tank；Then rotate rotating circular disk, the liquid in next liquid storage pipe is injected in micro-fluidic chip, carries out the most successively, until all courses of reaction are complete.
Fig. 1 shows the structural representation of the micro-fluidic detection device of the preferred embodiment of the present invention, wherein the chip slot 1-1 of base 1 is placed with a micro-fluidic chip, base 1 be arranged above sample adding device 2, rotating circular disk 2-1 is connected to the top of cylinder 2-2, multiple fixed bit, liquid storage pipe 2-3 is had to be placed in fixed bit along rotating circular disk 2-1 circumferencial direction.
Fig. 2 shows the structure of the base of the embodiment of the present invention, and wherein the size of chip slot 1-1 should adapt with the size of micro-fluidic chip to be detected.For different Detection tasks, micro-fluidic chip to be detected can be any micro-fluidic chip, and such as the micro-fluidic chip in S bend pipe road, micro-fluidic chip to be detected should have liquid injection port and liquid outlet.
Fig. 3 shows the structure of the sample adding device of the embodiment of the present invention, and wherein rotating circular disk 2-1 is connected to the top of cylinder 2-2, has multiple fixed bit, dismountable liquid storage pipe 2-3 to be placed in fixed bit along rotating circular disk 2-1 circumferencial direction.The material selection macromolecular material of liquid storage pipe 2-3, such as polypropylene PP.
In an embodiment of the present invention, the tip of liquid storage pipe 2-3 is placed with piezoelectric ceramics (not shown), is controlled the flowing of liquid storage liquid in pipe during detection by piezoelectric effect.
In an embodiment of the present invention, rotating circular disk 2-1 is connected with Zero-point positioning system (not shown), is controlled rotation and the location of rotating circular disk during detection by Zero-point positioning system.
Fig. 4 shows the structure of the micro-fluidic chip to be detected of the embodiment of the present invention, and it has liquid injection port 3 and liquid outlet 4.
When the micro-fluidic detection device utilizing the embodiment of the present invention detects, placing micro-fluidic chip in chip slot 1-1, the liquid injection port 3 of chip is below rotating circular disk 2-1, and cylinder 2-2 can be rotated disk 2-1 and move up and down；Fixing dismountable liquid storage pipe 2-3 on rotating circular disk 2-1, can adjust the quantity of liquid storage pipe 2-3 according to detection program；Use Zero-point positioning system to control the rotation of rotating circular disk 2-1, thus it is corresponding with detecting step to control liquid storage pipe 2-3；The tip of liquid storage pipe 2-3 is placed with piezoelectric ceramics, controls the flowing of liquid in liquid storage pipe 2-3 by piezoelectric effect and completes all processes of reaction.Hydraulic pump is accessed, by reacted waste liquid suction waste liquid tank 1-2 at liquid outlet 4.
Further, micro-fluidic detection device based on above example, can be this device configuration data acquisition module and data analysis module, acquisition testing signal carry out interpretation of result during detecting at micro-fluidic detection device.Specifically, the reaction signal of micro-fluidic chip in CCD camera shooting chip slot 1-1 can be used, such as chemiluminescence signal, then send the photo that CCD camera photographs to computer, utilize related software to be analyzed processing, obtain detecting analysis result.
Above, being described in detail technical scheme in conjunction with specific embodiments, described specific embodiment is adapted to assist in the thought understanding the present invention.Within derivation that those skilled in the art make on the basis of the specific embodiment of the invention and modification fall within scope.
Claims (8)
1. a micro-fluidic detection device, it is characterised in that including: base, sample adding device and negative pressure pump, sample adding device is positioned at above base, wherein,
There is on base chip slot and waste liquid tank；
Sample adding device includes rotating circular disk, cylinder and liquid storage pipe, wherein rotating circular disk and the fixing connection of cylinder；There is multiple fixed bit, during liquid storage pipe is positioned over fixed bit during use on rotating circular disk；
During detection, micro-fluidic chip to be detected is placed in chip slot, height by cylinder regulation rotating circular disk, and control rotating circular disk rotation, so that liquid storage pipe is positioned at the top of the liquid injection port of micro-fluidic chip, the liquid outlet of micro-fluidic chip is connected with negative pressure pump, and negative pressure pump is for injecting waste liquid in waste liquid tank.
Micro-fluidic detection device the most as claimed in claim 1, it is characterised in that wherein, the tip of described liquid storage pipe is placed with piezoelectric ceramics, for controlling the flowing of liquid storage liquid in pipe by piezoelectric effect.
Micro-fluidic detection device the most as claimed in claim 1 or 2, it is characterised in that wherein, described rotating circular disk connects Zero-point positioning system, is controlled rotation and the location of described rotating circular disk by Zero-point positioning system.
Micro-fluidic detection device the most as claimed in claim 1 or 2, it is characterised in that wherein, the size of described chip slot adapts with the size of micro-fluidic chip to be detected.
Micro-fluidic detection device the most as claimed in claim 1 or 2, it is characterised in that wherein, described micro-fluidic chip to be detected is the micro-fluidic chip in S bend pipe road.
6. a micro-fluidic detecting system, it is characterised in that include data acquisition module, data analysis module and the micro-fluidic detection device as according to any one of claim 1-5；Wherein,
Data acquisition module is acquisition testing signal during detecting at described micro-fluidic detection device；
Data analysis module carries out predetermined analyzing and processing for the detection signal collecting data acquisition module.
Micro-fluidic detecting system the most as claimed in claim 6, it is characterised in that wherein, described data acquisition module is CCD camera.
Micro-fluidic detecting system the most as claimed in claims 6 or 7, it is characterised in that wherein, described data analysis module is computer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610294161.5A CN105823727B (en) | 2016-05-05 | 2016-05-05 | A kind of micro-fluidic detection device and system |
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| CN201610294161.5A CN105823727B (en) | 2016-05-05 | 2016-05-05 | A kind of micro-fluidic detection device and system |
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| CN105823727B CN105823727B (en) | 2018-10-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113637580A (en) * | 2020-04-27 | 2021-11-12 | 复旦大学 | Multi-force field coupled cell tissue culture chip |
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