CN111721823A - Plant nutrient real-time detection system based on electrophoresis microfluidic technology - Google Patents
Plant nutrient real-time detection system based on electrophoresis microfluidic technology Download PDFInfo
- Publication number
- CN111721823A CN111721823A CN202010714198.5A CN202010714198A CN111721823A CN 111721823 A CN111721823 A CN 111721823A CN 202010714198 A CN202010714198 A CN 202010714198A CN 111721823 A CN111721823 A CN 111721823A
- Authority
- CN
- China
- Prior art keywords
- soil
- data
- nutrient
- nutrient content
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 57
- 238000005516 engineering process Methods 0.000 title claims abstract description 23
- 238000011897 real-time detection Methods 0.000 title claims abstract description 14
- 238000001962 electrophoresis Methods 0.000 title description 6
- 239000002689 soil Substances 0.000 claims abstract description 96
- 238000001514 detection method Methods 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000007405 data analysis Methods 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 12
- 238000013144 data compression Methods 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 235000021049 nutrient content Nutrition 0.000 claims description 48
- 239000000758 substrate Substances 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 12
- 239000000872 buffer Substances 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 238000005070 sampling Methods 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- 238000006073 displacement reaction Methods 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims description 3
- 238000002386 leaching Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 230000008859 change Effects 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 3
- 230000004720 fertilization Effects 0.000 abstract description 3
- 230000004907 flux Effects 0.000 abstract description 3
- 238000009472 formulation Methods 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000005370 electroosmosis Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000012530 fluid Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000004377 microelectronic Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000005527 soil sampling Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F11/00—Error detection; Error correction; Monitoring
- G06F11/07—Responding to the occurrence of a fault, e.g. fault tolerance
- G06F11/14—Error detection or correction of the data by redundancy in operation
- G06F11/1402—Saving, restoring, recovering or retrying
- G06F11/1446—Point-in-time backing up or restoration of persistent data
- G06F11/1458—Management of the backup or restore process
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Theoretical Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geology (AREA)
- Remote Sensing (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Quality & Reliability (AREA)
- Electrochemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a plant nutrient real-time detection system based on an electrophoretic micro-fluidic technology, which comprises a soil acquisition unit, a soil liquid extraction unit, a nutrient detection unit and a data analysis and processing unit; the data analysis processing unit comprises a data comparison module, a data compression module and a data backup module. Has the advantages that: the invention has the advantages of high flux, low consumption and less pollution when detecting the plant nutrients, and the micro-fluidic chip and the like are arranged, thereby reducing the detection time of the plant nutrients, improving the working efficiency, reducing the consumption of the detection and reducing the pollution of the detection to the environment; by arranging the rotating device, the flowing speed of the soil liquid in the micro-fluidic chip can be increased, and the detection efficiency is further improved; through setting up data analysis processing unit to can prevent that the data detected from leading to the fact the problem of data loss because of computer system trouble, guarantee to detect the change of knowing plant nutrient continuously, be favorable to the formulation of the plan of plant fertilization.
Description
Technical Field
The invention relates to the field of plant nutrient detection, in particular to a plant nutrient real-time detection system based on an electrophoretic micro-fluidic technology.
Background
Microfluidics refers to the science and technology involved in systems that process or manipulate tiny fluids (nanoliters to attoliters in volume) using microchannels (tens to hundreds of microns in size), and is an emerging interdiscipline that involves chemical, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Because of the miniaturization, integration, and other features, microfluidic devices are often referred to as microfluidic chips.
Important factors influencing plant growth are nutrients in soil near the plant, mainly comprising elements such as nitrogen, phosphorus and potassium, and the content of the elements cannot be too high or too low, otherwise, the elements can have negative effects on plant production. The traditional methods for detecting plant nutrients are usually a rapid test method and an ion collection method, but the methods can generate excessive waste liquid and are not environment-friendly. The existing problems can be effectively solved by adopting the electrophoretic microfluidic technology.
An effective solution to the problems in the related art has not been proposed yet.
Disclosure of Invention
Aiming at the problems in the related art, the invention provides a plant nutrient real-time detection system based on an electrophoretic micro-fluidic technology, so as to overcome the technical problems in the prior related art.
Therefore, the invention adopts the following specific technical scheme:
the system comprises a soil acquisition unit, a soil liquid extraction unit, a nutrient detection unit and a data analysis and processing unit; the soil collecting unit is used for collecting soil samples at different depths around the plant and sending the soil samples to a laboratory;
the soil liquid extraction unit is used for preparing the collected soil sample into soil liquid;
the nutrient detection unit is used for adding the prepared soil liquid into detection equipment, measuring the content of elements such as nitrogen, phosphorus and potassium in the soil liquid to obtain nutrient content data, and inputting the nutrient content data into a computer;
the data analysis processing unit comprises a data comparison module, a data compression module and a data backup module;
the data comparison module is used for comparing each group of nutrient content data with each other and comparing each group of nutrient content data with a soil nutrient content standard table to obtain the soil nutrient grade of each group of soil;
the data compression module is used for compressing and storing each group of nutrient content data to obtain compressed data;
the data backup module is used for backing up the compressed data.
Further, the soil collecting unit is used for collecting soil samples at different depths around the plant, and the step of sending the soil samples to the laboratory further comprises:
sampling soil with the depth of four layers of 0-10cm, 10-20cm, 20-30cm and 30-6-40cm around the plant, wherein the quality of each sample is the same;
repeatedly sampling sand soil with four layers of depth on the soil surface every 10cm, and taking five groups in total;
and (4) uniformly mixing the soil samples, filling the soil samples into a sealing tape, sealing the sealing tape, marking numbers, and sending the sealing tape to a laboratory.
Further, the step of preparing the soil liquid from the collected soil sample by the soil liquid extraction unit further comprises:
respectively putting the collected soil samples into a dryer, and drying at 105-115 ℃;
putting the dried soil sample into a dried conical flask, and adding a proper amount of 0.5MOL/L sodium bicarbonate leaching liquor;
stirring the soil sample by a glass rod, adding a proper amount of activated carbon, oscillating vigorously for 1-2 minutes, standing for 10 minutes, and filtering to obtain a soil solution.
Further, the data compression module is used for compressing and storing each group of nutrient content data, and the step of obtaining compressed data further comprises:
step one, acquiring nutrient content data and an initial acquisition time point of the nutrient content data, and setting the type of a fitting curve of the nutrient content data;
step two, obtaining an initial feasible region of nutrient content data according to the fitting curve;
acquiring first nutrient content data of a set time interval after an initial acquisition time point, and acquiring a first feasible region of the first nutrient content data according to a fitting curve;
judging whether an intersection exists between the initial feasible region and the first feasible region;
if the intersection exists, taking the intersection as an initial feasible region, taking the first nutrient content data as initial measurement data, and executing the third step;
if the intersection does not exist, obtaining a coordinate value in the initial feasible region, taking the coordinate value as a coefficient of a fitting curve, storing the coordinate value and the nutrient content data obtained at the initial time point in the step one, taking the first nutrient content data as encrypted declaration data, and executing the step one.
Further, the step of the data backup module for backing up the compressed data further includes:
generating a difference list between the target folder and the backup folder when a backup instruction for backing up the target folder to the backup folder is received;
calculating the difference proportion of at least difference data of the difference list in the target folder;
judging whether the difference proportion is larger than a specific proportion or not;
overwriting at least the target data of the target folder to the backup folder when the difference ratio is greater than the specific ratio so as to backup the target folder to the backup folder; and when the difference proportion is not larger than the specific proportion, backing up the target folder to the backup folder by using a difference backup program.
Furthermore, the nutrient detection unit comprises a base, a rotating device is arranged at the top end of the base, a microfluidic chip is arranged at the top end of the rotating device, an electrophoresis detector is arranged at the top end of the base and on one side of the microfluidic chip, and a computer is arranged on the side edge of the base.
Furthermore, in order to accelerate the flow speed of soil liquid in the microfluidic chip, the detection efficiency is improved, noise generated in rotation can be reduced, and the working environment of detection personnel is improved.
Furthermore, in order to improve the rotation precision of the microfluidic chip, angular displacement sensors are respectively arranged on the stator and the rotor.
Further, in order to reduce the detection time of plant nutrients, the work efficiency is improved, the consumption of detection can be reduced, and the pollution of detection to the environment can be reduced, the micro-fluidic chip comprises a base plate connected with the two tops of the rotating shaft, a plurality of groups of sample inlets are arranged on the base plate in a circumferential array mode, one side, away from the central position of the base plate, of each group of sample inlets is respectively provided with a buffer pool, one side, away from the sample inlets, of each group of buffer pools is respectively provided with a reaction pool, one end, away from the buffer pools, of each group of reaction pools is respectively provided with a gas outlet, and the two sides of.
Furthermore, in order to improve the electroosmosis property and the optical property of the microfluidic chip and further improve the detection speed and the detection precision of the nutrient detection unit, the substrate is made of glass.
The invention has the beneficial effects that:
the invention has the advantages of high flux, low consumption and less pollution when detecting the plant nutrients, and the micro-fluidic chip and the like are arranged, thereby reducing the detection time of the plant nutrients, improving the working efficiency, reducing the consumption of the detection and reducing the pollution of the detection to the environment; by arranging the rotating device, the flowing speed of the soil liquid in the micro-fluidic chip can be increased, and the detection efficiency is further improved; the substrate is made of glass, so that the electroosmosis property and the optical property of the microfluidic chip can be improved, and the detection speed and the detection precision of the nutrient detection unit are improved; through setting up data analysis processing unit to can prevent that the data detected from leading to the fact the problem of data loss because of computer system trouble, guarantee to detect the change of knowing plant nutrient continuously, be favorable to the formulation of the plan of plant fertilization.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram of a real-time plant nutrient detection system based on electrophoretic micro-fluidic technology according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a three-dimensional structure of a nutrient detection unit in a plant nutrient real-time detection system based on an electrophoretic micro-fluidic technology according to an embodiment of the present invention;
FIG. 3 is a schematic front structural diagram of a nutrient detection unit in a plant nutrient real-time detection system based on an electrophoretic micro-fluidic technology according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a pipeline of a microfluidic chip of a nutrient detection unit in a real-time plant nutrient detection system based on an electrophoretic microfluidic technology according to an embodiment of the present invention.
In the figure:
1. a soil collection unit; 2. a soil liquid extraction unit; 3. a nutrient detection unit; 4. a data analysis processing unit; 401. a data comparison module; 402. a data compression module; 403. a data backup module; 5. a base; 6. a rotating device; 601. a stator; 602. a rotor; 603. a connecting shaft; 604. mounting grooves; 605. a bearing; 606. a first rotating shaft; 607. a second rotating shaft; 608. an angular displacement sensor; 7. a microfluidic chip; 701. a substrate; 702. a sample inlet; 703. a buffer pool; 704. a reaction tank; 705. an air outlet; 706. positive and negative electrodes; 8. an electrophoresis detector; 9. and (4) a computer.
Detailed Description
For further explanation of the various embodiments, the drawings which form a part of the disclosure and which are incorporated in and constitute a part of this specification, illustrate embodiments and, together with the description, serve to explain the principles of operation of the embodiments, and to enable others of ordinary skill in the art to understand the various embodiments and advantages of the invention, and, by reference to these figures, reference is made to the accompanying drawings, which are not to scale and wherein like reference numerals generally refer to like elements.
According to the embodiment of the invention, a plant nutrient real-time detection system based on an electrophoretic micro-fluidic technology is provided.
Referring to the drawings and the detailed description, the invention will be further described, and as shown in fig. 1 to 4, a plant nutrient real-time detection system based on the electrophoretic micro-fluidic technology according to an embodiment of the invention includes a soil collection unit 1, a soil liquid extraction unit 2, a nutrient detection unit 3, and a data analysis processing unit 4; the soil collecting unit 1 is used for collecting soil samples at different depths around the plant and sending the soil samples to a laboratory;
the soil liquid extraction unit 2 is used for preparing soil liquid from the collected soil sample;
the nutrient detection unit 3 is used for adding the prepared soil liquid into detection equipment, measuring the content of elements such as nitrogen, phosphorus and potassium in the soil liquid to obtain nutrient content data, and inputting the nutrient content data into the computer 9;
the data analysis processing unit 4 comprises a data comparison module 401, a data compression module 402 and a data backup module 403;
the data comparison module 401 is configured to compare each group of nutrient content data with each other, and compare each group of nutrient content data with a soil nutrient content standard table to obtain a soil nutrient grade of each group of soil;
the data compression module 402 is configured to compress and store each group of nutrient content data to obtain compressed data;
the data backup module 403 is used for backing up the compressed data.
In one embodiment, the step of the soil sampling unit 1 for collecting soil samples at different depths around the plant and sending the soil samples to the laboratory further comprises:
sampling soil with the depth of four layers of 0-10cm, 10-20cm, 20-30cm and 30-6-40cm around the plant, wherein the quality of each sample is the same;
repeatedly sampling sand soil with four layers of depth on the soil surface every 10cm, and taking five groups in total;
and (4) uniformly mixing the soil samples, filling the soil samples into a sealing tape, sealing the sealing tape, marking numbers, and sending the sealing tape to a laboratory.
In one embodiment, the step of preparing the soil liquid from the collected soil sample by the soil liquid extraction unit 2 further comprises:
respectively putting the collected soil samples into a dryer, and drying at 105-115 ℃;
putting the dried soil sample into a dried conical flask, and adding a proper amount of 0.5MOL/L sodium bicarbonate leaching liquor;
stirring the soil sample by a glass rod, adding a proper amount of activated carbon, oscillating vigorously for 1-2 minutes, standing for 10 minutes, and filtering to obtain a soil solution.
In one embodiment, the data compression module 402 is configured to compress and store each set of nutrient content data, and the step of obtaining compressed data further includes:
step one, acquiring nutrient content data and an initial acquisition time point of the nutrient content data, and setting the type of a fitting curve of the nutrient content data;
step two, obtaining an initial feasible region of nutrient content data according to the fitting curve;
acquiring first nutrient content data of a set time interval after an initial acquisition time point, and acquiring a first feasible region of the first nutrient content data according to a fitting curve;
judging whether an intersection exists between the initial feasible region and the first feasible region;
if the intersection exists, taking the intersection as an initial feasible region, taking the first nutrient content data as initial measurement data, and executing the third step;
if the intersection does not exist, obtaining a coordinate value in the initial feasible region, taking the coordinate value as a coefficient of a fitting curve, storing the coordinate value and the nutrient content data obtained at the initial time point in the step one, taking the first nutrient content data as encrypted declaration data, and executing the step one.
In one embodiment, the step of the data backup module 403 for backing up the compressed data further includes:
generating a difference list between the target folder and the backup folder when a backup instruction for backing up the target folder to the backup folder is received;
calculating the difference proportion of at least difference data of the difference list in the target folder;
judging whether the difference proportion is larger than a specific proportion or not;
overwriting at least the target data of the target folder to the backup folder when the difference ratio is greater than the specific ratio so as to backup the target folder to the backup folder; and when the difference proportion is not larger than the specific proportion, backing up the target folder to the backup folder by using a difference backup program.
As shown in fig. 2-4, the nutrient detection unit 3 includes a base 5, a rotating device 6 is disposed on the top of the base 5, a microfluidic chip 7 is disposed on the top of the rotating device 6, an electrophoresis detector 8 is disposed on one side of the microfluidic chip 7 on the top of the base 5, and a computer 9 is disposed on the side of the base 5.
In an embodiment, for the above rotating device 6, the rotating device 6 includes a stator 601 disposed at the top end of the base 5, a rotor 602 is disposed inside the stator 601, a connecting shaft 603 is connected to the inside of the rotor 602, a mounting groove 604 is disposed in the base 5 and below the connecting shaft 603, a bearing 605 is disposed in the mounting groove 604, a first rotating shaft 606 is disposed between the bearing 605 and the bottom end of the connecting shaft 603, a second rotating shaft 607 is disposed at the top end of the connecting shaft 603, and the top end of the second rotating shaft 607 is connected to the microfluidic chip 7, so that the flowing speed of the soil liquid in the microfluidic chip 7 can be increased, the detection efficiency can be improved, the noise generated during rotation can be reduced, and the working environment of the detection personnel can.
In one embodiment, for the stator 601, the angular displacement sensor 608 is disposed on each of the stator 601 and the rotor 602, so as to improve the rotation accuracy of the microfluidic chip 7.
In an embodiment, for the above microfluidic chip 7, by arranging the microfluidic chip 7 to include the substrate 701 connected to the top end of the second rotating shaft 607, the substrate 701 is circumferentially arrayed with a plurality of groups of sample inlets 702, one side of each group of sample inlets 702 away from the center of the substrate 701 is respectively provided with the buffer pool 703, one side of each group of buffer pools 703 away from the sample inlets 702 is respectively provided with the reaction pool 704, one end of each group of reaction pools 704 away from the buffer pool 703 is respectively provided with the gas outlet 705, and two sides of each group of reaction pools 704 are respectively provided with the positive and negative electrodes 706, thereby reducing the detection time of plant nutrients, improving the work efficiency, and reducing the consumption of detection and the pollution of detection to the environment.
In one embodiment, for the substrate 701, the substrate 701 is made of glass, so that electroosmosis properties and optical properties of the microfluidic chip 7 can be improved, and detection speed and accuracy of the nutrient detection unit can be improved.
For the convenience of understanding the technical solutions of the present invention, the following detailed description will be made on the working principle or the operation mode of the present invention in the practical process.
In practical application, the prepared soil solution is added into the sample inlet 702 of the microfluidic chip 7 and flows into the buffer pool 703 and the reaction pool 704 in sequence. The stator 601 and the rotor 602 are electrified, a magnetic field is generated near the stator 601 and the rotor 602, the current in the rotor 602 and the magnetic field act, so that the rotor 602 rotates, the rotor drives the first rotating shaft 606 and the second rotating shaft 607 to rotate, the second rotating shaft 607 drives the microfluidic chip 7 to rotate, and the speed of soil liquid entering the reaction tank 704 is increased. The electrophoresis detector 8 detects the nutrients in the soil liquid.
In conclusion, by means of the technical scheme, the device has the advantages of high flux, low consumption and less pollution when detecting the plant nutrients, and the micro-fluidic chip and the like are arranged, so that the detection time of the plant nutrients is shortened, the working efficiency is improved, the detection consumption can be reduced, and the pollution of the detection to the environment is reduced; by arranging the rotating device 6, the flowing speed of the soil liquid in the micro-fluidic chip 7 can be increased, and the detection efficiency is further improved; the substrate 701 is made of glass, so that the electroosmosis property and the optical property of the microfluidic chip 7 can be improved, and the detection speed and the detection precision of the nutrient detection unit are improved; through setting up data analysis processing unit 4 to can prevent that the data loss's problem is caused because of computer system trouble to the testing data, guarantee to detect continuously and know the change of plant nutrient, be favorable to the formulation of the plan of plant fertilization.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "disposed," "connected," "secured," "screwed" and the like are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A plant nutrient real-time detection system based on an electrophoretic micro-fluidic technology is characterized by comprising a soil acquisition unit (1), a soil liquid extraction unit (2), a nutrient detection unit (3) and a data analysis processing unit (4);
the soil collecting unit (1) is used for collecting soil samples at different depths around a plant and sending the soil samples to a laboratory;
the soil liquid extraction unit (2) is used for preparing soil liquid from the collected soil sample;
the nutrient detection unit (3) is used for adding the prepared soil liquid into detection equipment, measuring the content of elements such as nitrogen, phosphorus and potassium in the soil liquid to obtain nutrient content data, and inputting the nutrient content data into the computer (9);
the data analysis processing unit (4) comprises a data comparison module (401), a data compression module (402) and a data backup module (403);
the data comparison module (401) is used for comparing each group of nutrient content data with each other, and comparing each group of nutrient content data with a soil nutrient content standard table to obtain the soil nutrient grade of each group of soil;
the data compression module (402) is used for compressing and storing each group of nutrient content data to obtain compressed data;
the data backup module (403) is used for backing up the compressed data.
2. The system for detecting the plant nutrients based on the electrophoretic micro-fluidic technology as claimed in claim 1, wherein the soil collection unit (1) is used for collecting soil samples at different depths around the plant, and the step of sending the soil samples to the laboratory further comprises:
sampling soil with the depth of four layers of 0-10cm, 10-20cm, 20-30cm and 30-6-40cm around the plant, wherein the quality of each sample is the same;
repeatedly sampling the sand soil with the depth of the four layers on the soil surface every 10cm, and taking five groups;
and (4) uniformly mixing the soil samples, filling the soil samples into a sealing tape, sealing the sealing tape, marking numbers, and sending the sealing tape to a laboratory.
3. The system for real-time detection of plant nutrients based on the electrophoretic micro-fluidic technology as claimed in claim 1, wherein the step of the soil liquid extraction unit (2) for preparing the collected soil sample into the soil liquid further comprises:
respectively putting the collected soil samples into a dryer, and drying at 105-115 ℃;
putting the dried soil sample into a dried conical flask, and adding a proper amount of 0.5MOL/L sodium bicarbonate leaching liquor;
stirring the soil sample by a glass rod, adding a proper amount of activated carbon, oscillating vigorously for 1-2 minutes, standing for 10 minutes, and filtering to obtain a soil solution.
4. The system for detecting the plant nutrients based on the electrophoretic micro-fluidic technology as claimed in claim 1, wherein the data compression module (402) is configured to compress and store each group of the nutrient content data, and the step of obtaining the compressed data further includes:
step one, acquiring nutrient content data and an initial acquisition time point of the nutrient content data, and setting the type of a fitting curve of the nutrient content data;
step two, obtaining an initial feasible region of the nutrient content data according to the fitting curve;
step three, obtaining first nutrient content data of a set time interval after the initial acquisition time point, and obtaining a first feasible region of the first nutrient content data according to the fitting curve;
judging whether an intersection exists between the initial feasible region and the first feasible region;
if the intersection exists, taking the intersection as an initial feasible region, taking the first nutrient content data as initial measurement data, and executing a third step;
if no intersection exists, obtaining a coordinate value in the initial feasible region, taking the coordinate value as a coefficient of a fitting curve, storing the coordinate value and the nutrient content data obtained at the initial time point in the first step, taking the first nutrient content data as the encrypted declaration data, and executing the first step.
5. The system for real-time detection of plant nutrients based on the electrophoretic micro-fluidic technology as claimed in claim 1, wherein the step of the data backup module (403) for backing up the compressed data further comprises:
generating a difference list between a target folder and a backup folder when a backup instruction for backing up the target folder to the backup folder is received;
calculating the difference proportion of at least difference data of the difference list in the target folder;
judging whether the difference proportion is larger than a specific proportion or not;
overwriting at least target data of a target folder to the backup folder when the difference ratio is greater than the specific ratio to backup the target folder to the backup folder; and when the difference proportion is not larger than the specific proportion, backing up the target folder to the backup folder by using a difference backup program.
6. The electrophoretic micro-fluidic technology based plant nutrient real-time detection system according to claim 1, wherein the nutrient detection unit (3) comprises a base (5), a rotating device (6) is arranged at the top end of the base (5), a micro-fluidic chip (7) is arranged at the top end of the rotating device (6), an electrophoretic detector (8) is arranged at the top end of the base (5) and on one side of the micro-fluidic chip (7), and the computer (9) is arranged at the side edge of the base (5).
7. The system for detecting the plant nutrients based on the electrophoretic micro-fluidic technology according to claim 6, wherein the rotating device (6) comprises a stator (601) arranged at the top end of the base (5), a rotor (602) is arranged on the inner side of the stator (601), a connecting shaft (603) is connected to the inner side of the rotor (602), a mounting groove (604) is arranged in the base (5) and below the connecting shaft (603), a bearing (605) is arranged in the mounting groove (604), a first rotating shaft (606) is arranged between the bearing (605) and the bottom end of the connecting shaft (603), a second rotating shaft (607) is arranged at the top end of the connecting shaft (603), and the top end of the second rotating shaft (607) is connected with the micro-fluidic chip (7).
8. The system for detecting the plant nutrients based on the electrophoretic micro-fluidic technology as claimed in claim 7, wherein angular displacement sensors (608) are respectively arranged on the stator (601) and the rotor (602).
9. The system for detecting the plant nutrients based on the electrophoretic micro-fluidic technology according to claim 6, wherein the micro-fluidic chip (7) comprises a substrate (701) connected with the top end of the second rotating shaft (607), a plurality of groups of sample inlets (702) are arranged on the substrate (701) in a circumferential array, one side of each group of the sample inlets (702) far away from the central position of the substrate (701) is respectively provided with a buffer pool (703), one side of each group of the buffer pools (703) far away from the sample inlets (702) is respectively provided with a reaction pool (704), one end of each group of the reaction pools (704) far away from the buffer pools (703) is respectively provided with a gas outlet (705), and both sides of each group of the reaction pools (704) are respectively provided with a positive electrode and a negative electrode (706).
10. The system for real-time detection of plant nutrients based on the electrophoretic micro-fluidic technology as claimed in claim 9, wherein the substrate (701) is made of glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010714198.5A CN111721823A (en) | 2020-07-22 | 2020-07-22 | Plant nutrient real-time detection system based on electrophoresis microfluidic technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010714198.5A CN111721823A (en) | 2020-07-22 | 2020-07-22 | Plant nutrient real-time detection system based on electrophoresis microfluidic technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111721823A true CN111721823A (en) | 2020-09-29 |
Family
ID=72573273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010714198.5A Pending CN111721823A (en) | 2020-07-22 | 2020-07-22 | Plant nutrient real-time detection system based on electrophoresis microfluidic technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111721823A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113753977A (en) * | 2021-09-06 | 2021-12-07 | 北京思源广泰科技有限公司 | Data processing method and system |
| CN115032370A (en) * | 2022-06-13 | 2022-09-09 | 中科合肥智慧农业协同创新研究院 | On-site detection device and detection method for soil nutrients |
| CN115598363A (en) * | 2022-12-01 | 2023-01-13 | 华侨大学（Cn） | Mechanical integrated automatic detection system and detection method for soil nutrients |
| WO2023240930A1 (en) * | 2022-06-13 | 2023-12-21 | 中科合肥智慧农业协同创新研究院 | Soil nutrient field test device, test method thereof, and micro-fluidic chip |
| WO2024007489A1 (en) * | 2022-07-05 | 2024-01-11 | 中科合肥智慧农业协同创新研究院 | On-site rapid detection device for soil organic matter, and detection method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101853194A (en) * | 2009-04-03 | 2010-10-06 | 纬创资通股份有限公司 | Data backup method |
| CN101902226A (en) * | 2009-05-25 | 2010-12-01 | 北京庚顿数据科技有限公司 | Data compression method |
| WO2012104870A1 (en) * | 2011-02-04 | 2012-08-09 | Council Of Scientific & Industrial Research | Molecularly imprinted conducting polymer film based aqueous amino acid sensors |
| CN104931551A (en) * | 2015-05-21 | 2015-09-23 | 西安交通大学 | Paper base micro-fluidic chip for selecting soil active bacterium and components and application of paper base micro-fluidic chip |
| CN105486568A (en) * | 2015-11-17 | 2016-04-13 | 郑州欧柯奇仪器制造有限公司 | Universal extraction agent for soil and soil nutrient extraction method |
| CN105717186A (en) * | 2016-02-01 | 2016-06-29 | 常熟理工学院 | Rotary type dielectrophoresis microfluidic analysis device |
| CN107561245A (en) * | 2017-09-04 | 2018-01-09 | 北京林业大学 | Large-size lake wetland of beach soil seed bank, soil seed pool species richness and seed density detection method |
| KR20180120001A (en) * | 2017-04-26 | 2018-11-05 | 광운대학교 산학협력단 | Biosensors for detecting Homocysteine and Cysteine |
| CN210221830U (en) * | 2019-07-04 | 2020-03-31 | 刘明远 | Application device of soil pre-nutrient-increasing device in forest planting and forestation |
| CN111141808A (en) * | 2020-01-20 | 2020-05-12 | 中国科学院合肥物质科学研究院 | Soil available state analysis method based on electrophoresis analysis technology and soil nutrient grade evaluation method thereof |
-
2020
- 2020-07-22 CN CN202010714198.5A patent/CN111721823A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101853194A (en) * | 2009-04-03 | 2010-10-06 | 纬创资通股份有限公司 | Data backup method |
| CN101902226A (en) * | 2009-05-25 | 2010-12-01 | 北京庚顿数据科技有限公司 | Data compression method |
| WO2012104870A1 (en) * | 2011-02-04 | 2012-08-09 | Council Of Scientific & Industrial Research | Molecularly imprinted conducting polymer film based aqueous amino acid sensors |
| CN104931551A (en) * | 2015-05-21 | 2015-09-23 | 西安交通大学 | Paper base micro-fluidic chip for selecting soil active bacterium and components and application of paper base micro-fluidic chip |
| CN105486568A (en) * | 2015-11-17 | 2016-04-13 | 郑州欧柯奇仪器制造有限公司 | Universal extraction agent for soil and soil nutrient extraction method |
| CN105717186A (en) * | 2016-02-01 | 2016-06-29 | 常熟理工学院 | Rotary type dielectrophoresis microfluidic analysis device |
| KR20180120001A (en) * | 2017-04-26 | 2018-11-05 | 광운대학교 산학협력단 | Biosensors for detecting Homocysteine and Cysteine |
| CN107561245A (en) * | 2017-09-04 | 2018-01-09 | 北京林业大学 | Large-size lake wetland of beach soil seed bank, soil seed pool species richness and seed density detection method |
| CN210221830U (en) * | 2019-07-04 | 2020-03-31 | 刘明远 | Application device of soil pre-nutrient-increasing device in forest planting and forestation |
| CN111141808A (en) * | 2020-01-20 | 2020-05-12 | 中国科学院合肥物质科学研究院 | Soil available state analysis method based on electrophoresis analysis technology and soil nutrient grade evaluation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 孙文志 等: "考虑养分变异性的最低成本饲料配方模型", 《动物营养学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113753977A (en) * | 2021-09-06 | 2021-12-07 | 北京思源广泰科技有限公司 | Data processing method and system |
| CN115032370A (en) * | 2022-06-13 | 2022-09-09 | 中科合肥智慧农业协同创新研究院 | On-site detection device and detection method for soil nutrients |
| WO2023240930A1 (en) * | 2022-06-13 | 2023-12-21 | 中科合肥智慧农业协同创新研究院 | Soil nutrient field test device, test method thereof, and micro-fluidic chip |
| WO2024007489A1 (en) * | 2022-07-05 | 2024-01-11 | 中科合肥智慧农业协同创新研究院 | On-site rapid detection device for soil organic matter, and detection method thereof |
| CN115598363A (en) * | 2022-12-01 | 2023-01-13 | 华侨大学（Cn） | Mechanical integrated automatic detection system and detection method for soil nutrients |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111721823A (en) | Plant nutrient real-time detection system based on electrophoresis microfluidic technology | |
| CN102784674A (en) | Centrifugal micro-fluidic chip for detecting chromium ion form in water body and preparation method of centrifugal micro-fluidic chip | |
| CN104001562B (en) | A kind of disk centrifugal type microfludic chip and device separating fast for chromium form | |
| CN102928389B (en) | Device for quickly detecting impregnation amount in real time and using method thereof | |
| CN104792769B (en) | The dynamic micro-extraction of compound, detection combined unit and method in aqueous sample | |
| CN205620290U (en) | Micro -fluidic detection device and system | |
| CN102788791A (en) | Fast determination method for soil acidity and alkalinity and determination device thereof | |
| CN107175090A (en) | A kind of porous carbon solid-phase micro-extraction coating and its preparation method and application | |
| CN107860694B (en) | Method for measuring migration rule of pre-crosslinked gel particles in rock microscopic pore throats | |
| CN107478468B (en) | Accessory of micro-area sampling instrument | |
| CN111076964B (en) | In-situ collection device and method for soluble substances | |
| CN1245621C (en) | Trace quantity sodium ion automatic rapid determination method and apparatus | |
| CN202854008U (en) | Experiment measuring equipment system for wettability of porous media interface | |
| CN111024584A (en) | Acid rock reaction double-rock-plate fracture-cave simulation device and working method thereof | |
| CN208223877U (en) | A kind of Sediment Pore Water original position high-resolution sampler | |
| CN102539500A (en) | Sensor based on asymmetric polymer film as well as detection method and applications thereof | |
| CN103245765A (en) | Ocean parameter measuring system | |
| CN212780211U (en) | PO-DGT of environmental multielement allies oneself with uses detection device | |
| CN104237045A (en) | Novel drainability tester | |
| CN204649479U (en) | Be applicable to the multifunction sampling pond of fermented sample process | |
| CN206930487U (en) | A kind of fully automatic liquid processing system | |
| CN109375286B (en) | A kind of self-operated measuring unit and method of Air-sea heat fluxes | |
| CN202946137U (en) | Digital indicator of pumping well based on multi-sensor information fusion | |
| CN213780084U (en) | Fast sampling and detecting device for obstacle factors of urban relocation area | |
| CN207067057U (en) | Portable USB interface heavy metal ion detection device and electrode card |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200929 |