CN105817455B - A kind of microflow controlled biochip cleaning device and cleaning needle - Google Patents
A kind of microflow controlled biochip cleaning device and cleaning needle Download PDFInfo
- Publication number
- CN105817455B CN105817455B CN201610294626.7A CN201610294626A CN105817455B CN 105817455 B CN105817455 B CN 105817455B CN 201610294626 A CN201610294626 A CN 201610294626A CN 105817455 B CN105817455 B CN 105817455B
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- cleaning
- cleaning needle
- needle
- chip
- liquid injection
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- 238000004140 cleaning Methods 0.000 title claims abstract description 155
- 238000000018 DNA microarray Methods 0.000 title claims abstract description 31
- 239000007788 liquid Substances 0.000 claims description 40
- 238000002347 injection Methods 0.000 claims description 31
- 239000007924 injection Substances 0.000 claims description 31
- 238000005406 washing Methods 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 3
- 230000003014 reinforcing effect Effects 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 230000037452 priming Effects 0.000 claims 3
- 238000000034 method Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 4
- 239000004579 marble Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B11/00—Cleaning flexible or delicate articles by methods or apparatus specially adapted thereto
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Cleaning By Liquid Or Steam (AREA)
Abstract
The invention discloses a kind of microflow controlled biochip cleaning device, including：Base, cleaning needle fixed mount and cleaning needle；Wherein, base includes horizontal positioned bottom plate and the side plate being vertically installed on bottom plate；Cleaning needle fixed mount is arranged on side plate, and can be moved up and down along side plate plate face；Also, there are multiple cleaning needle fixed bits, for fixing cleaning needle on cleaning needle fixed mount；Cleaning needle is placed in cleaning needle fixed bit during use, and microflow controlled biochip to be cleaned is fixed on bottom plate, and the position of cleaning needle is corresponding with the position of chip.The invention also discloses a kind of cleaning needle.The present invention can exclude the interference of human factor as the dedicated cleaner of microflow controlled biochip.
Description
Technical Field
The invention relates to the technical field of immunodiagnosis detection, in particular to a micro-fluidic biochip cleaning device and a cleaning needle.
Background
The microfluidic chip analysis technology is one of the current science and technology leading fields, mainly uses analytical chemistry, controls fluid in a micron-sized structure, performs reaction detection, and has the advantages of small volume, less reagent and sample consumption, capability of simultaneously detecting multiple samples and multiple indexes and the like. In the past, the detection needs a sample amount of a few milliliters, only needs a sample amount of a few microliters after the micro-fluidic chip technology is adopted, so that the consumption of samples and reagents is greatly saved, the performance index is superior to that of a common detection method, and the method has wide application prospect and important application value.
In the field of immunodiagnostic testing, microfluidic chip analysis techniques can be used for in vitro immunodiagnostic testing, generally comprising the steps of: coating antibody, sealing, loading, cleaning, adding enzyme-labeled secondary antibody, cleaning and reading by an instrument. In actual test operation, in order to ensure the accuracy and success rate of the test, a plurality of cleaning steps are required. At present, due to the lack of a special cleaning device, most of test operations are finished by adopting a method of manually cleaning a spray gun, a large amount of time and energy are consumed, most importantly, human factors existing in the manual cleaning process can influence the test results, for example, the manual cleaning process has the condition of insufficient cleaning or excessive cleaning, the next process can be influenced, and further the whole test result is adversely affected.
Disclosure of Invention
In view of the above, the present invention provides a microfluidic biochip cleaning apparatus and a cleaning needle, which can be used as a dedicated cleaning apparatus to eliminate the interference of human factors.
In one aspect, the present invention provides a microfluidic biochip cleaning apparatus, comprising: the cleaning device comprises a base, a cleaning needle fixing frame and a cleaning needle; the base comprises a bottom plate which is horizontally arranged and a side plate which is vertically arranged on the bottom plate; the cleaning needle fixing frame is arranged on the side plate and can move up and down along the plate surface of the side plate; the cleaning needle fixing frame is provided with a plurality of cleaning needle fixing positions for fixing the cleaning needles; when the device is used, the cleaning needle is arranged in the cleaning needle fixing position, the microfluidic biochip to be cleaned is fixed on the bottom plate, and the position of the cleaning needle corresponds to the position of the chip.
Preferably, the side plate is provided with a linear guide rail, the linear guide rail is arranged along the vertical direction, and the cleaning needle fixing frame is arranged on the guide rail through a guide rail connecting block.
Preferably, the end of the linear guide rail is provided with a limiting block for limiting the moving amplitude of the cleaning needle fixing frame.
Preferably, the device further comprises a liquid injection tool, the bottom plate is provided with a liquid injection tool groove, and the liquid injection tool is installed in the liquid injection tool groove; wherein, annotate and have chip supporting mechanism on the liquid frock, the micro-fluidic biochip that treats the washing when using is installed on chip supporting mechanism.
Preferably, annotate one side of liquid frock groove and be provided with the marble fixed block, fix the position of annotating the liquid frock through the marble fixed block during the installation.
Preferably, one side of the liquid injection tool is provided with a guide mechanism, and the liquid injection tool is installed in the liquid injection tool groove through the guide mechanism during installation.
Preferably, a chip baffle is arranged on the other side of the liquid injection tool and used for limiting the position of the chip installed in the chip supporting mechanism.
Preferably, the cleaning needle comprises a cleaning needle supporting part and a cleaning needle body, the cleaning needle supporting part is sleeved on the cleaning needle body, and the cleaning needle is fixed in the cleaning needle fixing position through the cleaning needle supporting part.
Preferably, one or more adjusting bolts are arranged on the cleaning needle supporting part, and the relative positions of the cleaning needle supporting part and the cleaning needle body are adjusted through the adjusting bolts.
Preferably, the cleaning needle fixing frame is provided with at least two rows of cleaning needle fixing positions, when in use, the position of one row of cleaning needles in the at least two rows of cleaning needle fixing positions corresponds to the liquid injection port of the chip, and the position of the other row of cleaning needles in the at least two rows of cleaning needle fixing positions corresponds to the waste liquid outlet of the chip.
Preferably, a vacuum negative pressure device is connected to the upper end of the cleaning needle.
In another aspect, the present invention further provides a cleaning needle, comprising a cleaning needle support portion and a cleaning needle body; wherein, wash needle supporting part cover and establish on wasing the needle body, wash and be provided with one or more adjusting bolt on the needle supporting part, through adjusting bolt adjustment washs the relative position of needle supporting part and washing needle body.
Aiming at the defects of the prior art, the embodiment of the invention provides the micro-fluidic biochip cleaning device which can be operated in batch, saves time and energy and has strong controllability. The device provided by the embodiment of the invention has the advantages of simple structure, convenience in processing and convenience in operation; a motor can be arranged on the base according to the requirement, so that the automatic operation is realized; in actual use, the number of the cleaning needles can be adjusted according to the number of the biochips, so that small-batch simultaneous cleaning is realized, the influence of the cleaning process on the batch difference is eliminated, the precision of the cleaning process can be improved, the interference of human factors is eliminated, and the cleaning time can be greatly saved.
Drawings
FIG. 1 is a schematic structural view of a microfluidic biochip cleaning apparatus according to a preferred embodiment of the present invention.
Fig. 2 is a front view of a base of an embodiment of the present invention.
Fig. 3 is a rear view of a base of an embodiment of the present invention.
Fig. 4 is a schematic structural diagram of a liquid injection tool according to an embodiment of the present invention.
Fig. 5 is a schematic view showing a state in which the cleaning needle is placed on the cleaning needle holder according to the embodiment of the present invention.
Fig. 6 is a schematic structural view of a cleaning needle holder according to an embodiment of the present invention.
Fig. 7 is a schematic structural view of a cleaning needle according to an embodiment of the present invention.
Detailed Description
The technical solution of the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.
The microfluidic biochip cleaning device of the embodiment of the invention comprises: the cleaning device comprises a base, a cleaning needle fixing frame and a cleaning needle, wherein the base comprises a horizontally placed bottom plate and a side plate vertically arranged on the bottom plate; the cleaning needle fixing frame is arranged on the side plate and can move up and down along the plate surface of the side plate; the cleaning needle fixing frame is provided with a plurality of cleaning needle fixing positions for fixing the cleaning needles; when the device is used, the cleaning needle is arranged in the cleaning needle fixing position, the microfluidic biochip to be cleaned is fixed on the bottom plate, and the position of the cleaning needle corresponds to the position of the chip.
In practical application, no matter a single or a plurality of micro-fluidic biochips to be cleaned can be fixed on the bottom plate, and a corresponding number of cleaning needles are placed in the cleaning needle fixing frame.
Fig. 1 shows a schematic structural diagram of a microfluidic biochip cleaning apparatus according to a preferred embodiment of the present invention, in which a cleaning needle holder 2 is installed on a side plate of a base 1, and a liquid injection tool 3 is disposed on a bottom plate of the base 1 for fixing a microfluidic biochip to be cleaned. Wherein, a plurality of cleaning needles are arranged in the cleaning needle fixing frame 2.
Fig. 2 shows a schematic structural diagram of a base according to an embodiment of the present invention, in which a bottom plate 1-1 of the base 1 is horizontally disposed, and side plates 1-2 are vertically mounted on the bottom plate 1-1. The side plates 1-2 are provided with two linear guide rails which are arranged along the vertical direction, the cleaning needle fixing frame 2 can be arranged on the guide rails through the guide rail connecting blocks 1-5, and then the cleaning needle fixing frame 2 can move up and down along the surface of the side plates. And a limiting block 1-6 can be arranged at a proper position of the upper end part of the linear guide rail, so that the upward moving amplitude of the cleaning needle fixing frame 2 can be limited. In the figure 2, a liquid injection tool groove 1-3 on a bottom plate 1-1 is used for installing and containing a liquid injection tool 3, and the position of the liquid injection tool 3 is fixed by a marble fixing block 1-4.
Fig. 3 shows a back structure of the base according to the embodiment of the present invention, a plurality of reinforcing ribs 1-8 may be additionally installed between the side plate 1-2 and the bottom plate 1-1, and the reinforcing ribs 1-8 mainly play a role of fixedly supporting the side plate 1-2.
Fig. 4 shows a structure of a liquid injection tool according to an embodiment of the present invention, the liquid injection tool 3 has a chip support 3-2, and a microfluidic biochip to be cleaned is mounted on the chip support 3-2, so that the position of the chip is fixed. One side of the liquid injection tool 3 is provided with a guide strip 3-3, and the liquid injection tool 3 is installed in the liquid injection tool groove 1-3 through the guide strip 3-3. And a chip baffle 3-1 is arranged on the other side of the liquid injection tool 3, so that the position of the chip can be limited in an auxiliary manner, and the chip is prevented from moving.
Fig. 5 is a schematic view showing a state where the cleaning needle of the embodiment of the present invention is placed on the cleaning needle holder, and fig. 6 and 7 show the structures of the cleaning needle holder and the cleaning needle, respectively. The cleaning needle fixing frame of the preferred embodiment is plate-shaped, the plate surface is provided with at least two rows of cleaning needle fixing positions, for example, the embodiment of fig. 5 and 6 is two rows, and three rows, four rows or more cleaning needle fixing positions can be arranged according to actual requirements.
Referring to fig. 7, the structure of a single cleaning needle is used in cooperation with a cleaning needle holder. The cleaning needle comprises a cleaning needle support 2-2 and a cleaning needle body 2-3, the cleaning needle support 2-2 is sleeved on the cleaning needle body 2-3, one or more adjusting bolts 2-2-1 are arranged on the cleaning needle support 2-2, and the cleaning needle body 2-3 can be adjusted to a desired state by adjusting the relative positions of the cleaning needle support 2-2 and the cleaning needle body 2-3 through the adjusting bolts 2-2-1. The cleaning needle is integrally fixed in the cleaning needle fixing position through the cleaning needle support 2-2 and fixed by a fastening bolt. When cleaning, the upper end part of the cleaning needle is connected with a vacuum negative pressure device, so that automatic cleaning can be realized. The cleaning needle provided by the embodiment of the invention is simple in structure and easy to install.
In actual operation, a microfluidic biochip to be cleaned is placed in the chip support 3-2, then the whole liquid injection tool 3 is placed in the liquid injection tool groove 1-3, and the position of the liquid injection tool is fixed through the marble fixing block 1-4; the negative pressure device is connected on washing needle upper portion, and manual or motor control washs needle mount 2 and moves down, and the adjustment washs the needle so that the back row of washing needle corresponds liquid injection mouth and the waste liquid export of biochip respectively, forms closed loop, starts external negative pressure device and can accomplish the washing, and later manual or motor control washs needle mount 2 rebound, and it can to push out notes liquid frock 3.
The technical solutions of the present invention have been described in detail with reference to specific embodiments, which are used to help understand the ideas of the present invention. The derivation and modification made by the person skilled in the art on the basis of the specific embodiment of the present invention also belong to the protection scope of the present invention.
Claims (9)
1. A microfluidic biochip cleaning device, comprising: the cleaning device comprises a base, a cleaning needle fixing frame and a cleaning needle; wherein,
the base comprises a horizontally arranged bottom plate, a side plate vertically arranged on the bottom plate and a reinforcing rib arranged between the side plate and the bottom plate;
the cleaning needle fixing frame is arranged on the side plate and can move up and down along the plate surface of the side plate; the cleaning needle fixing frame is provided with a plurality of cleaning needle fixing positions for fixing the cleaning needles;
when in use, the cleaning needle is arranged in the cleaning needle fixing position, the microfluidic biological chip to be cleaned is fixed on the bottom plate, and the position of the cleaning needle corresponds to the position of the chip;
the cleaning needle comprises a cleaning needle supporting part and a cleaning needle body, the cleaning needle supporting part is sleeved on the cleaning needle body, the cleaning needle is fixed in a cleaning needle fixing position through the cleaning needle supporting part, one or more adjusting bolts are arranged on the cleaning needle supporting part, and the adjusting bolts adjust the relative positions of the cleaning needle supporting part and the cleaning needle body.
2. The microfluidic biochip cleaning apparatus according to claim 1, wherein the side plates have linear guides extending in a vertical direction, and the cleaning pin holder is mounted on the guides via a guide connection block.
3. The microfluidic biochip cleaning apparatus according to claim 2, wherein the linear guide has a stopper at an end thereof for limiting the movement of the cleaning pin holder.
4. The microfluidic biochip cleaning device of claim 1, further comprising a liquid injection tool, wherein the bottom plate has a liquid injection tool slot, and the liquid injection tool is mounted in the liquid injection tool slot; wherein, annotate and have chip supporting mechanism on the liquid frock, the micro-fluidic biochip that treats the washing when using is installed on chip supporting mechanism.
5. The microfluidic biochip cleaning apparatus according to claim 4, wherein a ball fixing block is disposed on one side of the liquid injection tool groove, and the position of the liquid injection tool is fixed by the ball fixing block during installation.
6. The microfluidic biochip cleaning apparatus of claim 4, wherein the priming tool has a guide mechanism on one side, and the priming tool is mounted in the priming tool slot via the guide mechanism during mounting.
7. The microfluidic biochip cleaning apparatus according to claim 6, wherein the liquid injection tool has a chip stopper on the other side thereof for limiting the position of the chip mounted in the chip support mechanism.
8. The microfluidic biochip cleaning apparatus according to any of claims 1 to 7, wherein the cleaning pin holder has at least two rows of cleaning pin holding locations, wherein in use, one of the at least two rows of cleaning pin holding locations corresponds to the liquid inlet of the chip, and the other of the at least two rows of cleaning pin holding locations corresponds to the waste liquid outlet of the chip.
9. The microfluidic biochip cleaning apparatus according to any one of claims 1 to 7, wherein a vacuum negative pressure device is connected to an upper end of the cleaning needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610294626.7A CN105817455B (en) | 2016-05-05 | 2016-05-05 | A kind of microflow controlled biochip cleaning device and cleaning needle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610294626.7A CN105817455B (en) | 2016-05-05 | 2016-05-05 | A kind of microflow controlled biochip cleaning device and cleaning needle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105817455A CN105817455A (en) | 2016-08-03 |
| CN105817455B true CN105817455B (en) | 2018-04-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610294626.7A Active CN105817455B (en) | 2016-05-05 | 2016-05-05 | A kind of microflow controlled biochip cleaning device and cleaning needle |
Country Status (1)
| Country | Link |
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| CN (1) | CN105817455B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107583918B (en) * | 2017-08-25 | 2019-08-20 | 博奥生物集团有限公司 | A kind of semi-automated biological device for cleaning chip |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090230044A1 (en) * | 2008-03-13 | 2009-09-17 | Agilent Technologies, Inc. | Microfluid Chip Cleaning |
| CN201519677U (en) * | 2009-09-28 | 2010-07-07 | 内蒙古科技大学包头医学院 | Ultrasonic cleaner for micropipettor suction head |
| CN103212552B (en) * | 2013-04-10 | 2015-10-14 | 上海裕隆生物科技有限公司 | Equipment for drying is dipped in a kind of point sample instrument syringe needle cleaning |
| CN104492742B (en) * | 2015-01-06 | 2017-02-22 | 北京九州泰康生物科技有限责任公司 | Biochip cleaning device |
| CN105344639A (en) * | 2015-11-06 | 2016-02-24 | 江苏三联生物工程有限公司 | Biological chip washing mechanism |
| CN205613817U (en) * | 2016-05-05 | 2016-10-05 | 北京纳迅科技股份有限公司 | Micro -fluidic biochip belt cleaning device and washing needle |
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2016
- 2016-05-05 CN CN201610294626.7A patent/CN105817455B/en active Active
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| CN105817455A (en) | 2016-08-03 |
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