CN2702313Y - Biological chip production and chip-on biomolecule fixing and detection integrated system - Google Patents
Biological chip production and chip-on biomolecule fixing and detection integrated system Download PDFInfo
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- CN2702313Y CN2702313Y CN 200420064457 CN200420064457U CN2702313Y CN 2702313 Y CN2702313 Y CN 2702313Y CN 200420064457 CN200420064457 CN 200420064457 CN 200420064457 U CN200420064457 U CN 200420064457U CN 2702313 Y CN2702313 Y CN 2702313Y
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Abstract
The utility model relates to a biological chip producing, sample adding and the reaction detecting integrated system. The utility model comprises an elastic material piece is provided with a groove, an opening hole which is provided on the both sides of the groove, and a rigid material block which is opened with a round hole. The opening position of the round hole is corresponding to the through hole of the elastic material piece. The surface of the elastic material piece without groove is adhered with the rigid material block together, and the hole of the elastic material piece is connected with the hole of the rigid material block. The chip is arranged on the elastic material piece, and the surface of the chip is correspondingly contacted with the groove. Another rigid material block is provided with a hole, and a test tube is provided in the hole. The test tube is correspondingly arranged with the liquid outlet which is connected with the micro pump. The utility model is provided with an adjustable sample platform support. The rigid material block which is provided on the support, is provided with the round hole, and the round hole is corresponding to the through hole of the elastic material plate. Each channel of the micro pump is connected with the micro-flow passage liquid inlet or outlet micro tubes of the chip. The system designs the liquid transporting unit, which causes the liquor of the each flow passage to simultaneously and uniformly flow into the micro-flow passage in the same time, and the flowing compatibility and the evenness of each flow passage are held.
Description
Technical field
The utility model relates to a kind of micro sample-adding of biomolecule chip and the method and device thereof of reaction of being used for, and particularly relates to a kind ofly collecting that biochip is made and directly carrying out on this biochip that biomolecule is fixed and the incorporate device of detection reaction immediately.
Background technology
The biomolecule chip is a kind of integrated parallel Measurement for Biotechnique that just grew up in recent years, can integrated multiple aglucon on small geometric scale, so just can be simultaneously the multiple index of micro-example be detected.Because biomolecule sample price height requires consumption the least possible, therefore require employed biomolecule reagent of biomolecule chip and sample traceization, this also just requires chip application of sample and reaction unit microminiaturization.What at present, the application of sample of biochip mainly adopted is point sample instrument.Difference according to the point sample mode is divided into two classes, and a class is a contact, at first dip in and get stand-by aglucon with spotting needle, then by the contact chip surface aglucon point on chip; One class is the spray printing formula, draws a spot of aglucon for the treatment of a little with hollow spotting needle earlier, and the mode by similar ink-jet printer is added to aglucon on the chip surface then.The common drawback of this dual mode is that the point sample amount is inhomogeneous, and the surface density of the interior aglucon molecule of a single point distributes also inhomogeneous, and this will have a strong impact on the quality of testing result, and be difficult to quantification.Current, what biochip reaction adopted is the W-response mode mostly, exactly whole being immersed in the testing sample solution of chip is reacted.The testing sample solution amount that this method needs is more, and the reaction time is long, and sensitivity is not high.
Another chip application of sample and reaction technology are to adopt the fluid channel technology.At present, generally the chip of the fluid channel technology of Shi Yonging is incorporate, be that chip and fluid channel are to be produced on the same block of material, as document 1:Dielectrophoretic cell separation and gene expression profiling onmicroelectronic chip arrays.July 15,2002 Huang Y, Joo S, Duhon M, HellerM, Wallace B, Xu X, Anal Chem 2002 Jul 15; 74 (14): described among the 3362-71.The employed fluid channel of this method is disposable use, and this fluid channel is made complicated, and cost is higher, makes to use to be restricted.By the chip and the fluid channel technology of separating, fluid channel is reused, reduce cost greatly.But for the solution uniformity that makes each runner the fluid channel of flowing through, reach the flow state uniformity of chip each point, reduce because flowing velocity or inconsistent influence to the chip detection result successively need be further improved the fluid channel transport system.
Summary of the invention
The purpose of this utility model is in order to overcome the shortcoming of above-mentioned prior art, for the cost of manufacture that reduces biochip significantly with simplify the technology of the fixing and detection reaction of biomolecule, improve the flow of solution homogeneity of each runner simultaneously, reduce because flowing velocity or inconsistent influence successively, collect that biochip is made and directly carry out on this biochip that biomolecule is fixed and the incorporate device of detection reaction immediately thereby provide a kind of to the chip detection result.
The purpose of this utility model is achieved in that
The utility model provides a kind ofly collects that biochip is made and directly carry out on this biochip that biomolecule is fixed and the incorporate system of detection reaction immediately, comprising: one has the microchannel chip unit that the rigid material piece 4 of porous is formed by at least one chip block 1, at least one elastic material board 3 and one;
Described elastic material board 3 is made the groove 2 that is arrayed on its surface, each groove 2 two ends has through hole 10, as liquid-inlet 7 and outlet 8, makes fluid channel template (as shown in Figure 1a) respectively;
Described rigid material piece 4 has circular hole 5 on it, this circular hole 5 is by arrayed, and the distance between its circular hole 5 equals the length (shown in Fig. 3 a) of the groove 2 on the elastic material board 3; The position that this circular hole 5 is opened is corresponding with the through hole 10 of elastic material sheet, and the hole is big or small identical; Described elastic material sheet 3 is not bonded together with the face and the rigid material piece 4 of groove, and the groove 2 on its elastic material sheet 3 is outside, and the hole on two block of material communicates; Chip 1 is placed on the elastic material sheet 3, and its chip 1 surface and groove 2 relative closely contacts form cavity 9 one by one; Each cavity has 7, one outlets of an import, 8 (shown in Fig. 2 a), and import 7 and outlet 8 respectively are connected with a microtubule; Import and outlet microtubule are arranged (as shown in Figure 4) in order; It is characterized in that:
At least one collects the waste collection unit that waste liquid constitutes with the rigid material piece 6 and the test tube of test tube by being used to place; Have aperture 20 in order on this solid pieces of material 6, can place test tube in the aperture 20 and collect waste liquid; The liquid outlet that is communicated with micro pump is oppositely arranged with the test tube that the collection waste liquid is used;
Also comprise a rigid material piece 16 that is used to place sample, have circular hole 19 on this rigid material piece 16, this circular hole 19 is by arrayed, the through hole 10 as liquid-inlet of its circular hole 19 corresponding elastic material board 3 upper grooves 2; Place the container that contains a kind of protein sample in each circular hole 19, this rigid material piece 16 is installed on the example platform 14; And:
At least one sample stage support, this sample stage support is fixed a pole 21 by on a base 11 and its, gear 12 is installed pole one side and knob 13 is formed rotating mechanisms, by gear 12 with a sample platform 14 and pole vertical fixing; This platform 14 is placed rigid material piece 16; Regulate the height of example platform 14 by turning knob 13 driven gears 12;
At least one has multichannel micro pump 15, and the fluid channel liquid-inlet on each passage of micro pump 15 and the protein-chip unit or the microtubule of outlet are communicated with (as shown in Figure 4) with micro pump; By the governor motion of micro pump 15, regulator solution rate of flow of fluid, flow wait the set time of protein sample on chip or the time of reaction controlled.
Perhaps also comprise a fluid channel template 3 ', have through hole on it, the position and the number of through hole are set as required; The face of this elastic material board 3 ' band groove 2 is buckled on the elastic material board 3, its through hole is communicated with a part of liquid-inlet 7 and liquid outlet 8 on the elastic material board 3 respectively, the groove 2 of elastic material board 3 ' is communicated with all the other liquid-inlets 7 on the elastic material board 3 and liquid outlet 8, forms the fluid channel (shown in Fig. 2 b) of having only an import and an outlet.
Described container is the pipe that centrifuge tube etc. holds biological sample.
During use, the base material of chip 1 is installed on the protein-chip unit, it is closely contacted with elastic material sheet 3, and compress fluid channel by external force, the container of placing solution places in the porous rigid material block 16 on the sample stage support, regulate the sample stage height by rotating turning knob 13, can make that the protein solution in the sequentially-placed container contacts on import microtubule 17 and the porous rigid material block 16; The liquid level consistance by keeping protein solution in each container and the consistance of each import microtubule 17 length of protein-chip, when pump drove, protein solution can flow into each fluid channel at one time.
The outlet microtubule 18 of protein-chip system is collected waste liquid with in the test tube with being connected to by pump, under pump drives, protein solution by in the container through the import microtubule 17 chip cavity 9 of flowing through, the aglucon molecule arrives at the chip unit internal fixation, unnecessary albumen flows out through the outlet microtubule with solution, flows into waste collection system at last.This device realizes that the ground mechanization of solution uniformity transports, thereby assurance flow of solution homogeneity makes chip detection unanimity as a result;
Described elastic material sheet comprises: silica gel, rubber, plastics or other rubber-like material.
Described rigid material block of material comprises: materials such as plastics, metal, glass, its thickness 1mm is to 10mm.
Described groove is a strip groove, and its strip groove area is from 0.01mm
2-1mm
2The degree of depth is from 10 μ m-1mm; At least 2 of the numbers of its strip groove for example can be from 2-1000.
Described through hole internal diameter can be from 10 μ m to 1mm.
The internal diameter of described fluid channel can be from 1 μ m to 1mm.
Described microtubule comprises: poly-four fluorine tube, plastic tube, silicone tube, stainless-steel tube etc., basic internal diameter can be from 1 μ m to 10mm.
Described pump can be hyperchannel micro pumps such as peristaltic pump, ram pump.
Advantage of the present utility model is:
The system that the utility model provides is a kind of improved system, it is owing to increase application of sample unit and liquid transport unit in existing device, made biochip earlier so have in a device, carried out immediately on this chip that biomolecule is fixed and the function of detection reaction.
Utilize system of the present utility model independently to carry out application of sample simultaneously to multiple spot; The controlled amount of application of sample amount and point; The fixing zone of aglucon biomolecule is that strictness is limited by groove on the chip, fixing and reacted surface is washed through damping fluid again, the size of the point on the feasible chip of making and the biomolecule surface density uniformity in the point can effectively improve the detection quality.The chip reaction is limited in the tiny area, and under flow state, has quickened the mass transfer rate of biomolecule, has shortened the reaction time effectively, has improved sensitivity.Regulate by the height of sample stage support knob simultaneously, can change solution easily.In system of the present utility model again owing to increased the liquid transport unit, make the solution of each runner evenly flow into fluid channel at one time simultaneously, the consistance and the homogeneity that have kept each flow passage can reduce because the inhomogeneous generation chip detection error that flows.Method of the present utility model can make the biochip preparation and be reflected in the same device and realize, and can reuse, and effectively reduces the preparation expense of chip.
Description of drawings
The structural plan synoptic diagram of elastic material sheet 3 in Fig. 1 a the utility model device
Fig. 1 b is the A-A ' cross section view of Fig. 1 a
Fig. 2 a is that synoptic diagram (expression is used for that sample is fixed or the structure drawing of device of each point when carrying out detection reaction separately) is formed in first kind of microchannel chip unit in the utility model device
Fig. 2 b is that synoptic diagram (represent the structure drawing of device when each point carries out detection reaction with same solution, the through hole at elastic material sheet 3 groove two ends and the through hole of another elastic material sheet 3 ' corresponding the communicating of staggering forms fluid channel cooperation synoptic diagram) is formed in second kind of microchannel chip unit in the utility model device
The rigid material piece floor map of the microchannel chip of Fig. 3 a the utility model device
A-A ' the cross section view of Fig. 3 b Fig. 3 a
Microchannel chip cell side view in Fig. 4 the utility model device
Fig. 5 is that system of the present utility model (have microchannel chip unit, sample stage support, and the unit of liquid transport and waste collection) forms synoptic diagram
Drawing is described as follows:
1-chip 2-groove
3-elastic material board 3 '-elastic material board
The circular hole of the rigid material piece 5-microchannel chip rigid material piece of 4-microchannel chip
6-collects waste liquid rigid material piece 7-testing liquid import
8-testing liquid outlet 9-cavity
Through hole 11-base on the 10-elastic material board
12-gear 13-knob
14-platform 15-micro pump
16-places the rigid material piece 17-import microtubule of sample
18-outlet microtubule 19-places the circular hole of the rigid material piece of sample
20-collects the circular hole 21-pole of waste liquid with the rigid material piece
Embodiment
Referring to Fig. 1 a and Fig. 1 b; Getting a silica gel sheet is elastic material sheet 3, makes 12 grooves 2 on this silica gel sheet, and the groove area is 1mm
2, be 0.1mm deeply; Groove 2 two ends have through hole 10, and the internal diameter of its through hole 10 is 0.5mm.
Referring to Fig. 3 a and Fig. 3 b, a rigid material piece 4 is an organic glass, and having the aperture on it is the through hole 5 of 0.5mm, and the position that this through hole 5 is opened is corresponding with the through hole 10 of elastic material sheet 3.
Referring to Fig. 2 a, get a silicon base as chip 1, make one and of the present inventionly have a microchannel chip unit (expression is used for that sample is fixed or the structure drawing of device of each point when carrying out detection reaction separately); Chip 1 is placed on the face of elastic material sheet 3 band grooves 2, its chip 1 surface and groove 2 relative closely contacts form cavity 9 one by one; Each cavity has 7, one outlets of an import, 8 (shown in Fig. 2 a).
Referring to Fig. 4, elastic material sheet 3 is not placed on rigid material piece 4 with the face of groove is placed on, the through hole 5 of this rigid material piece 4 communicates with the through hole 10 of elastic material sheet 3, and compresses silicon 1.Silicon 1 closely contacts with the face of elastic material sheet 3 band grooves, and forms 12 annular seal spaces 9, promptly forms 12 fluid channel, and its fluid channel internal diameter is 0.5mm, and each cavity has two microtubule runners of one-in-and-one-out mouth; An import microtubule 17 is a stainless-steel tube, and outlet microtubule 18 front portion are divided into polyfluortetraethylene pipe, the part by pump 15 connects into silicone tube, and the silicone tube part directly picks out behind each passage by peristaltic pump 15 in the outlet microtubule 18.Pump 15 is for containing the peristaltic pump of 12 passages.Chip 1 material is a silicon.Fluid channel internal diameter 0.5mm.
Referring to Fig. 5; Make the embodiment of an a kind of system of the present utility model
A rigid material piece 16 of placing sample is an organic glass, and having the aperture on it is the through hole 19 of 60mm, and the position that this through hole 19 is opened is corresponding with the position of import microtubule 17.Placement fills 12 centrifuge tubes of 12 kinds of solution respectively in the through hole 19, and the centrifuge tube volume is 0.2ml.
A sample stage support of making of metallic aluminium, this sample stage support is by a base 11, with fix a pole 21 on it, pole 21 1 sides are equipped with the rotating mechanism that gear 12 and knob 13 formed, by the example platform 14 and pole 21 vertical fixing of gear 12 with a metallic aluminium; Place the rigid material piece 16 of setting-out product on this platform 14; Regulate the height of example platform 14 by turning knob 13 driven gears 12; Its adjustable-height scope is 0-8cm.
One places the waste collection unit that the rigid material piece 6 of collecting the waste liquid test tube is organic glass and test tube formation; Have 12 through holes 20 on its rigid material piece 6, can place test tube in the aperture 20 and collect waste liquid; The position of each through hole is with corresponding through outlet microtubule 18 positions behind the peristaltic pump.Place the centrifuge tube of 1.5ml in the through hole 20, outlet microtubule 18 ends are put on the centrifuge tube.
Referring to the device of Fig. 5 made, and carry out the fixing and reaction of 12 kinds of protein thereon.
(1) as shown in Figure 5, silicon chip is installed in the chip system.
(2) placement fills 12 centrifuge tubes of 12 kinds of protein solutions (concentration is respectively 0.1mg/ml) respectively in the through hole 19 of sample stage, and the centrifuge tube volume is 0.2ml, regulates sample stage knob 13, and import microtubule 17 is immersed in the protein solutions.And make the length unanimity of each import microtubule, each centrifuge tube height unanimity of sample stage, so each import microtubule 17 inserts the degree of depth unanimity of centrifuge tubes.
(3) under the driving of peristaltic pump 15,12 kinds of protein solutions are transported to zones of different on the silicon chip by fluid channel respectively, flow speed control is in 1 mul/min, 10 minutes time;
(4) then, centrifuge tube is replaced with the centrifuge tube that phosphate buffer is housed, carries phosphate buffer to the chip surface of above-mentioned steps (3) preparation by fluid channel, flow velocity is 10 mul/min, washes the biomolecule that is not fixed on the silicon chip surface;
(5) centrifuge tube is replaced with the centrifuge tube (every pipe contains 20 microlitres liquid to be measured) that contains testing sample is housed, carry liquid to be measured to the chip surface of above-mentioned steps (4) preparation by fluid channel, flow velocity is 10 mul/min, liquid to be measured and chip reaction back outflow.
(6) repeating step (4) washes unconjugated biomolecule.Damping fluid that all outlet ports microtubule 18 flows out and the liquid to be measured that reacted all flow into to be collected in the waste liquid centrifuge tube.
(7) take off chip, nitrogen dries up the back and observes.
Referring to Fig. 2 b, make the chip unit that another kind of the present utility model has cascaded structure; Promptly also comprise a fluid channel template 3 ', have through hole on it, the position of through hole and number are set according to the lead to the hole site of elastic material board 3 and number.The through hole at elastic material sheet 3 groove two ends and the through hole of another elastic material sheet 3 ' corresponding the communicating of staggering forms fluid channel and cooperates, the face of this elastic material board 3 ' band groove 2 is buckled on the elastic material board 3, its through hole is communicated with a part of liquid-inlet 7 and liquid outlet 8 on the elastic material board 3 respectively, the groove 2 of elastic material board 3 ' is communicated with all the other liquid-inlets 7 on the elastic material board 3 and liquid outlet 8, forms the fluid channel (shown in Fig. 2 b) of having only an import and an outlet.
Other parts are identical with embodiment 1.
Claims (9)
1. one kind collects the fixing system that detects one that reaches of biomolecule on biochip making and the chip, comprise: one by making the groove 2 that is arrayed at least one chip block 1, at least one surface, and each groove 2 two ends has elastic material board 3 and microchannel chip unit that the rigid material piece with porous 4 is formed of through hole 10;
Described porous rigid material block 4 has circular hole 5 on it, this circular hole 5 is by arrayed, and the position that this circular hole 5 is opened is corresponding with the through hole 10 of elastic material sheet, and the hole is big or small identical; Described elastic material sheet 3 is not bonded together with the face and the rigid material piece 4 of groove, and the groove 2 on its elastic material sheet 3 is outside, and the hole on two block of material communicates; Chip 1 is placed on the elastic material sheet 3, and its chip 1 surface and groove 2 relative closely contacts form cavity 9 one by one; Each cavity has 7, one outlets 8 of an import, and import 7 and outlet 8 respectively are connected with a microtubule; Import and outlet microtubule are arranged in order; It is characterized in that:
Comprise that also at least one collects the waste collection unit that waste liquid constitutes with the rigid material piece 6 and the test tube of test tube by being used to place; Have hole 20 on this solid pieces of material 6, place the test tube of collecting waste liquid in the hole 20; And the liquid outlet that test tube is communicated with micro pump is oppositely arranged;
A rigid material piece 16 that is used to place sample has circular hole 19 on this rigid material piece 16, and this circular hole 19 is by arrayed, a through hole of its circular hole 19 corresponding elastic material board 3 upper grooves 2; Place a kind of container of protein sample in each circular hole 19, this rigid material piece 16 is installed on the example platform 14; With:
At least one sample stage support, this sample stage support is fixed a pole 21 by on a base 11 and its, gear 12 is installed pole one side and knob 13 is formed rotating mechanisms, by gear 12 with a sample platform 14 and pole vertical fixing; This platform 14 is placed rigid material piece 16; Regulate the height of example platform 14 by turning knob 13 driven gears 12; And
At least one has multichannel micro pump 15, and the fluid channel liquid-inlet on each passage of micro pump 15 and the protein-chip unit or the microtubule of outlet are communicated with.
2. by the fixing system that detects one that reaches of biomolecule on described collection biochip making of claim 1 and the chip, it is characterized in that: also comprise a fluid channel template 3 ', have through hole on it, the position and the number of through hole are set as required; The face of this elastic material board 3 ' band groove 2 is buckled on the elastic material board 3, its through hole is communicated with a part of liquid-inlet 7 and liquid outlet 8 on the elastic material board 3 respectively, the groove 2 of elastic material board 3 ' is communicated with all the other liquid-inlets 7 on the elastic material board 3 and liquid outlet 8, forms the fluid channel of having only an import and an outlet.
3. make by claim 1 or 2 described collection biochips and chip on the system of the fixing and detection one of biomolecule, it is characterized in that: described container is that centrifuge tube or other hold the pipe of biological sample.
4. by the fixing system that detects one that reaches of biomolecule on claim 1 or 2 described collection biochips making and the chip, it is characterized in that: described rigid material block of material comprises: materials such as plastics, metal, glass, its thickness 1mm is to 10mm.
5. by the fixing system that detects one that reaches of biomolecule on claim 1 or 2 described collection biochips making and the chip, it is characterized in that: described groove is a strip groove, and its strip groove area is from 0.01mm2-1mm2; The degree of depth is from 10 μ m-1mm; At least 2 of the numbers of its strip groove.
6. by the fixing system that detects one that reaches of biomolecule on claim 1 or 2 described collection biochips making and the chip, it is characterized in that: described through hole internal diameter is that 10 μ m are to 1mm.
7. by the fixing system that detects one that reaches of biomolecule on claim 1 or 2 described collection biochips making and the chip, it is characterized in that: the internal diameter of described fluid channel is that 1 μ m is to 1mm.
8. by the fixing system that detects one that reaches of biomolecule on claim 1 or 2 described collection biochips making and the chip, it is characterized in that: described microtubule comprises: poly-four fluorine tube, plastic tube, silicone tube, stainless-steel tube, its internal diameter is that 1 μ m is to 10mm.
9. by the fixing system that detects one that reaches of biomolecule on claim 1 or 2 described collection biochips making and the chip, it is characterized in that: described pump comprises peristaltic pump, ram pump or hyperchannel micro pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420064457 CN2702313Y (en) | 2004-06-11 | 2004-06-11 | Biological chip production and chip-on biomolecule fixing and detection integrated system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420064457 CN2702313Y (en) | 2004-06-11 | 2004-06-11 | Biological chip production and chip-on biomolecule fixing and detection integrated system |
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| CN2702313Y true CN2702313Y (en) | 2005-05-25 |
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|---|---|---|---|
| CN 200420064457 Expired - Fee Related CN2702313Y (en) | 2004-06-11 | 2004-06-11 | Biological chip production and chip-on biomolecule fixing and detection integrated system |
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| CN100392316C (en) * | 2006-03-27 | 2008-06-04 | 博奥生物有限公司 | Flow structure of controlling liquid continuously flowing in micro-pipeline |
| CN100392317C (en) * | 2006-03-27 | 2008-06-04 | 博奥生物有限公司 | Flow structure of controlling liquid continuously flowing in micro-pipeline |
| WO2008083526A1 (en) * | 2007-01-10 | 2008-07-17 | Capitalbio Corporation | Microfluidic devices and methods for multiple analyte detection |
| CN102250751A (en) * | 2011-03-22 | 2011-11-23 | 博奥生物有限公司 | Interface device for biochip |
| CN102879593A (en) * | 2011-07-11 | 2013-01-16 | 张兆麟 | Multichannel liquid sample reagent rapid charging reactor |
| CN104049029A (en) * | 2014-07-02 | 2014-09-17 | 重庆大学 | Multi-channel magneto-elastic sensor detection chip |
| CN106226540A (en) * | 2016-08-31 | 2016-12-14 | 贵州金玖生物技术有限公司 | Full-automatic protein chip analyzer |
| CN106248979A (en) * | 2016-08-31 | 2016-12-21 | 贵州金玖生物技术有限公司 | Disposable reaction liquid suction reactor |
| CN109406813A (en) * | 2018-12-14 | 2019-03-01 | 贵州大学 | A kind of microfluidic control device for high-throughput droplet array microfluid point sample |
| CN109613295A (en) * | 2018-12-14 | 2019-04-12 | 贵州大学 | A kind of high throughput droplet array microfluid point sample control device |
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| WO2024152158A1 (en) * | 2023-01-16 | 2024-07-25 | 王锦弘 | Biochip manufacturing method and product thereof, and testing method using biochip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100392316C (en) * | 2006-03-27 | 2008-06-04 | 博奥生物有限公司 | Flow structure of controlling liquid continuously flowing in micro-pipeline |
| CN100392317C (en) * | 2006-03-27 | 2008-06-04 | 博奥生物有限公司 | Flow structure of controlling liquid continuously flowing in micro-pipeline |
| WO2008083526A1 (en) * | 2007-01-10 | 2008-07-17 | Capitalbio Corporation | Microfluidic devices and methods for multiple analyte detection |
| US8003063B2 (en) | 2007-01-10 | 2011-08-23 | Capitalbio Corporation | Microfluidic devices and methods for multiple analyte detection |
| CN102250751A (en) * | 2011-03-22 | 2011-11-23 | 博奥生物有限公司 | Interface device for biochip |
| CN102250751B (en) * | 2011-03-22 | 2013-05-22 | 博奥生物有限公司 | Interface device for biochip |
| CN102879593A (en) * | 2011-07-11 | 2013-01-16 | 张兆麟 | Multichannel liquid sample reagent rapid charging reactor |
| CN102879593B (en) * | 2011-07-11 | 2014-09-03 | 张兆麟 | Multichannel liquid sample reagent rapid charging reactor |
| CN104049029A (en) * | 2014-07-02 | 2014-09-17 | 重庆大学 | Multi-channel magneto-elastic sensor detection chip |
| CN104049029B (en) * | 2014-07-02 | 2017-05-10 | 重庆大学 | Multi-channel magneto-elastic sensor detection chip |
| CN106226540A (en) * | 2016-08-31 | 2016-12-14 | 贵州金玖生物技术有限公司 | Full-automatic protein chip analyzer |
| CN106248979A (en) * | 2016-08-31 | 2016-12-21 | 贵州金玖生物技术有限公司 | Disposable reaction liquid suction reactor |
| CN106226540B (en) * | 2016-08-31 | 2018-09-14 | 贵州金玖生物技术有限公司 | Full-automatic protein chip analyzer |
| CN109406813A (en) * | 2018-12-14 | 2019-03-01 | 贵州大学 | A kind of microfluidic control device for high-throughput droplet array microfluid point sample |
| CN109613295A (en) * | 2018-12-14 | 2019-04-12 | 贵州大学 | A kind of high throughput droplet array microfluid point sample control device |
| CN109613294A (en) * | 2018-12-14 | 2019-04-12 | 贵州大学 | A kind of high throughput droplet array microfluid spotting fluid sample feeding device |
| WO2024152158A1 (en) * | 2023-01-16 | 2024-07-25 | 王锦弘 | Biochip manufacturing method and product thereof, and testing method using biochip |
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