CN105802857A - Liquid culture medium of phellinus igniarius strains and preparation method of liquid culture medium as well as fermentation method of phellinus igniarius strains - Google Patents
Liquid culture medium of phellinus igniarius strains and preparation method of liquid culture medium as well as fermentation method of phellinus igniarius strains Download PDFInfo
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Abstract
The invention discloses a liquid culture medium of phellinus igniarius strains and a preparation method of the liquid culture medium as well as a fermentation method of the phellinus igniarius strains. The culture medium is prepared from the following raw materials in parts by weight: 10g/L to 25g/L of glucose, 30g/L to 50g/L of soybean cake powder, 1g/L to 5g/L of KH2PO4 and 1g/L to 2g/L of MgSO4, and the pH value is 5.5 to 6.5. The culture medium provided by the invention is simple in components and reasonable in formula and proportion; particularly, the soybean cake powder is added into the culture medium, so that the growth and development of hyphae can be accelerated and the activity of the strains is improved, and furthermore, the yield of the hyphae is improved and the yield of dry hyphae can reach 13.5g/L or more than 13.5g/L; the culture medium is the liquid culture medium and has simple formula and components, so that the method for preparing the culture medium is simple to operate, convenient to prepare and low in cost, and is suitable for industrial large-scale production.
Description
Technical field
The present invention relates to Phellinus igniarius (L. ex Fr.) Quel. strain fermentation field, be specifically related to the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, preparation method and fermentation process thereof.
Background technology
Phellinus igniarius (L. ex Fr.) Quel. (InonotussanghuangShengH.Wu, T.Hatt.&Y.C.Dai) is a newly discovered novel species, and Mycobank is numbered: 563091[1].Taxonomy belongs to Fungi mycota, Basidiomycota Basidiomycota, Agaricomycotina gill fungi subphylum, Agaricomycetes gill fungi guiding principle, Hymenochaetales rust leather pore fungi order, Hymenochaetaceae Hymenochaetaceae, Inonotus fibre shelf fungus belongs to, it is grown on the trunk of Morus, with host, there is specificity.
Phellinus igniarius (L. ex Fr.) Quel. initial stage picture block loess, through growth after a while, appearance is as the tongue stretched out on stub.Sporophore is perennial, the raw stockless in side, hard wooden, hemispherical ungulate, flat or irregular shape, capping is shallow liver brown to taupe or black, there is fine hair at the beginning, often have after old and significantly chap in length and breadth, have concentric ring rib, edge is blunt, having yellow flange, bacterial context is faint yellow or light coffee color, wooden, tube multilamellar, level is inconspicuous.Phellinus igniarius (L. ex Fr.) Quel. is different because of its parasitic seeds, and its shape, color and the composition contained are also different.At present, the Phellinus igniarius (L. ex Fr.) Quel. that market is sold has mulberry Phellinus igniarius (L. ex Fr.) Quel., pinaster Phellinus igniarius (L. ex Fr.) Quel., willow Phellinus igniarius (L. ex Fr.) Quel., Betula platyphylla Suk. Phellinus igniarius (L. ex Fr.) Quel. etc., and wherein mulberry Phellinus igniarius (L. ex Fr.) Quel. is superfine product, and price is the highest.
Phellinus igniarius (L. ex Fr.) Quel. be a kind of preciousness with fungus, have the laudatory title of " forest gold "." Chinese medicine voluminous dictionary " is recorded, sporophore is used as medicine, the gynaecopathias such as metrorrhagia, stranguria with blood, leukorrhagia, amenorrhea can be controlled, the effects such as it is antibacterial, anticancer, fibrosis, antioxidation are also very notable, thus become the study hotspot in international research field, particularly Japan and Korea S, carried out research extensively and profoundly to it.Owing to Phellinus igniarius (L. ex Fr.) Quel. is expensive and supply falls short of demand; necessarily cause the people's unordered collection to Phellinus igniarius (L. ex Fr.) Quel. bacterium; particularity and complexity plus the physiological ecological of Phellinus igniarius (L. ex Fr.) Quel. bacterium own; and the restriction of external condition; the Phellinus igniarius (L. ex Fr.) Quel. sporophore formed in nature is very rare; there is problems of natural Phellinus igniarius (L. ex Fr.) Quel. scarcity of resources, face exhaustion, it is difficult to meet the market demand.So utilizing biological engineering that it is carried out liquid fermentation mycelium production and artificial culture research is extremely important, but it is no matter artificial culture sporophore or liquid fermentation mycelium produces, it is thus achieved that the excellent species of high-quality is crucial.At present, prior art adopts comprehensive PD culture medium to cultivate, but there is the problem yielded poorly, and adopts PD culture medium to can only achieve the dry mycelium/1000ml of 6.0-7.8g.
Summary of the invention
It is an object of the invention to overcome the shortcoming of prior art, it is provided that the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, preparation method and fermentation process thereof.
The purpose of the present invention is achieved through the following technical solutions: the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, and it is made up of the raw material of following concentration:
Preferably, it is made up of the raw material of following concentration:
A kind of preparation method of the fluid medium of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps:
S1. each raw material is weighed by above-mentioned formula proportion, standby；
S2. by soybean cake powder adds water boil 25～35min, filter, take filtrate, filtrate adds glucose, KH2PO4、MgSO4, heated and stirred mix homogeneously also loads in 300ml triangular flask after adjusting pH value, and every bottled amount is 200ml, seals with gauze；
S3. at 115～121 DEG C, 0.069～0.103MPa sterilization treatment 30～45min, the fluid medium of Phellinus igniarius (L. ex Fr.) Quel. strain is namely prepared.
The fermentation process of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps
S1. accessing Phellinus igniarius (L. ex Fr.) Quel. fermented bacterium under gnotobasis in the fluid medium of preparation, the inoculum concentration of every bottle is the 10～20% of liquid spawn gross weight, and the concentration of liquid spawn is 1～2%；
S2. being placed on agitator by triangular flask and carry out fermentation culture after inoculation, the temperature of described fermentation culture is 25～30 DEG C, and incubation time is 10～15d, and the rotating speed of agitator is 120～150rad/min.
The invention have the advantages that medium component provided by the invention is simple, formula and reasonable mixture ratio, especially culture medium with the addition of soybean cake powder, can promote that mycelial growth is grown, improve the activity of bacterial strain, therefore improve mycelium production, dry mycelium production is up to more than 13.5g/L；Owing to the culture medium of the present invention is that fluid medium, formula components are simple, therefore, the method preparing this culture medium is simple to operate, preparation is convenient, cost is low, it is adaptable to industrialization large-scale production.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated:
Embodiment 1: the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it is made up of the raw material of following concentration:
Embodiment 2: the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it is made up of the raw material of following concentration:
Embodiment 3: the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it is made up of the raw material of following concentration:
Embodiment 4: the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it is made up of the raw material of following weight parts:
Embodiment 5: the preparation method of the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps:
S1. each raw material is weighed by above-mentioned formula proportion, standby；
S2. by soybean cake powder adds water boil 25min, filter, take filtrate, filtrate adds glucose, KH2PO4、MgSO4, heated and stirred mix homogeneously also loads in 300ml triangular flask after adjusting pH value, and every bottled amount is 200ml, seals with gauze；
S3. at 115 DEG C, 0.069MPa sterilization treatment 30min, the fluid medium of Phellinus igniarius (L. ex Fr.) Quel. strain is namely prepared.
Embodiment 6: the preparation method of the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps:
S1. each raw material is weighed by above-mentioned formula proportion, standby；
S2. by soybean cake powder adds water boil 35min, filter, take filtrate, filtrate adds glucose, KH2PO4、MgSO4, heated and stirred mix homogeneously also loads in 300ml triangular flask after adjusting pH value, and every bottled amount is 200ml, seals with gauze；
S3. at 121 DEG C, 0.103MPa sterilization treatment 45min, the fluid medium of Phellinus igniarius (L. ex Fr.) Quel. strain is namely prepared.
Embodiment 7: the preparation method of the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps:
S1. each raw material is weighed by above-mentioned formula proportion, standby；
S2. by soybean cake powder adds water boil 30min, filter, take filtrate, filtrate adds glucose, KH2PO4、MgSO4, heated and stirred mix homogeneously also loads in 300ml triangular flask after adjusting pH value, and every bottled amount is 200ml, seals with gauze；
S3. at 118 DEG C, 0.082MPa sterilization treatment 38min, the fluid medium of Phellinus igniarius (L. ex Fr.) Quel. strain is namely prepared.
Embodiment 8: the fermentation process of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps
S1. accessing Phellinus igniarius (L. ex Fr.) Quel. fermented bacterium under gnotobasis in the fluid medium of preparation, the inoculum concentration of every bottle is the 10% of liquid spawn gross weight, and the concentration of liquid spawn is 1%；
S2. being placed on agitator by triangular flask and carry out fermentation culture after inoculation, the temperature of described fermentation culture is 25 DEG C, and incubation time is 10d, and the rotating speed of agitator is 120rad/min.
Embodiment 9: the fermentation process of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps
S1. accessing Phellinus igniarius (L. ex Fr.) Quel. fermented bacterium under gnotobasis in the fluid medium of preparation, the inoculum concentration of every bottle is the 20% of liquid spawn gross weight, and the concentration of liquid spawn is 2%；
S2. being placed on agitator by triangular flask and carry out fermentation culture after inoculation, the temperature of described fermentation culture is 30 DEG C, and incubation time is 15d, and the rotating speed of agitator is 150rad/min.
Embodiment 10: the fermentation process of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain, it comprises the following steps
S1. accessing Phellinus igniarius (L. ex Fr.) Quel. fermented bacterium under gnotobasis in the fluid medium of preparation, the inoculum concentration of every bottle is the 15% of liquid spawn gross weight, and the concentration of liquid spawn is 1.5%；
S2. being placed on agitator by triangular flask and carry out fermentation culture after inoculation, the temperature of described fermentation culture is 28 DEG C, and incubation time is 13d, and the rotating speed of agitator is 135rad/min.
Embodiment 11: the optimum formula option screening experiment of fermentation medium
Adopting intiutive analysis method to be analyzed orthogonal experiments, result is in Table 1, and the impact order of mycelial biomass is D > C > B > A by factor A, B, C, D, and the impact order of yield of extracellular polysaccharide is B > D > C > A；The optimum combination of mycelial biomass is A1B3C3D3, but more excellent it is combined as A by what variance calculated1B1C2D3, under the same conditions two being verified property of combination are tested, that mycelial biomass is the highest is still A1B3C3D3Combination；The optimum combination of extracellular polysaccharide is A2B3C2D1, consistent with variance result of calculation.A1B3C3D3Combination mycelial biomass pole is significantly higher than A2B3C2D1, but yield of extracellular polysaccharide is more or less the same, so at A1B3C3D3The mycelial biomass of condition is all higher with two indexs of yield of extracellular polysaccharide.Therefore, comprehensive these two aspects considers, optimal medium formula is: glucose 15g/L, soybean cake powder 50g/L, KH2PO43g/L, MgSO41.5g/L。
Table 1: orthogonal and result
Claims (4)
1. the fluid medium of a Phellinus igniarius (L. ex Fr.) Quel. strain, it is characterised in that it is made up of the raw material of following concentration:
Glucose: 10～25g/L；Soybean cake powder: 30～50g/L；
KH2PO4: 1～5g/L；MgSO4: 1～2g/L；
PH is 5.5～6.5.
2. the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain as claimed in claim 1, it is characterised in that it is made up of the raw material of following concentration:
Glucose: 18g/L；Soybean cake powder: 40g/L；
KH2PO4: 3g/L；MgSO4: 1.5g/L；
PH is 6.
3. the preparation method of the fluid medium of a kind of Phellinus igniarius (L. ex Fr.) Quel. strain as claimed in claim 1 or 2, it is characterised in that it comprises the following steps:
S1. each raw material is weighed by above-mentioned formula proportion, standby；
S2. by soybean cake powder adds water boil 25～35min, filter, take filtrate, filtrate adds glucose, KH2PO4、MgSO4, heated and stirred mix homogeneously also loads in 300ml triangular flask after adjusting pH value, and every bottled amount is 200ml, seals with gauze；
S3. at 115～121 DEG C, 0.069～0.103MPa sterilization treatment 30～45min, the fluid medium of Phellinus igniarius (L. ex Fr.) Quel. strain is namely prepared.
4. the fermentation process of a Phellinus igniarius (L. ex Fr.) Quel. strain, it is characterised in that it comprises the following steps
S1. accessing Phellinus igniarius (L. ex Fr.) Quel. fermented bacterium under gnotobasis in the fluid medium of preparation, the inoculum concentration of every bottle is the 10～20% of liquid spawn gross weight, and the concentration of liquid spawn is 1～2%；
S2. being placed on agitator by triangular flask and carry out fermentation culture after inoculation, the temperature of described fermentation culture is 25～30 DEG C, and incubation time is 10～15d, and the rotating speed of agitator is 120～150rad/min.
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| CN115287197A (en) * | 2022-06-01 | 2022-11-04 | 中华全国供销合作总社济南果品研究所 | Millet fermentation phellinus igniarius strain and preparation method of millet phellinus igniarius fermentation product |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115287197A (en) * | 2022-06-01 | 2022-11-04 | 中华全国供销合作总社济南果品研究所 | Millet fermentation phellinus igniarius strain and preparation method of millet phellinus igniarius fermentation product |
| CN115287197B (en) * | 2022-06-01 | 2024-02-02 | 中华全国供销合作总社济南果品研究所 | Millet fermented Phellinus linteus strain and preparation method of millet Phellinus linteus fermented product |
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Application publication date: 20160727 |