CN105794646A - Double-layer tissue culture room - Google Patents

Double-layer tissue culture room Download PDF

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Publication number
CN105794646A
CN105794646A CN201610214289.6A CN201610214289A CN105794646A CN 105794646 A CN105794646 A CN 105794646A CN 201610214289 A CN201610214289 A CN 201610214289A CN 105794646 A CN105794646 A CN 105794646A
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CN
China
Prior art keywords
room
mistress
group training
interior room
double
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610214289.6A
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Chinese (zh)
Inventor
何志铿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Foshan Rongxintong Enterprise Consultation Services Co Ltd
Original Assignee
Foshan Rongxintong Enterprise Consultation Services Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Foshan Rongxintong Enterprise Consultation Services Co Ltd filed Critical Foshan Rongxintong Enterprise Consultation Services Co Ltd
Priority to CN201610214289.6A priority Critical patent/CN105794646A/en
Publication of CN105794646A publication Critical patent/CN105794646A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention provides a double-layer tissue culture room and belongs to the field of plant tissue culture equipment. The tissue culture room comprises an outer room and an inner room, wherein the outer room comprises a changing room and a sterilization system; the inner room comprises tissue culture frames, detection systems, temperature control systems, tissue culture light source systems and lighting systems. Ultraviolet irradiation is adopted for sterilization for a long time in the outer room, a technician can stay temporarily in the outer room firstly when entering and leaving the room and enters the inner room after wearing sterile clothing, and the probability of carrying outside substances adverse to the tissue culture environment into the tissue culture room is decreased to a certain extent. The double-layer tissue culture room has the advantages of low cost, high sterilization efficiency and low environmental bacteria density.

Description

A kind of double-deck group training room
Technical field
The invention belongs to plant tissue culture apparatus field, particularly relate to a kind of double-deck group training room.
Background technology
Tissue culture technique is to be placed in culture medium by work organ, tissue or cell under sterile conditions, and it is placed in suitable environment, carry out the cell of cultivation, tissue or individuality continuously, it mainly utilizes cellular omnipotency, namely each cell has the hereditary material of complete set, inducing the plant tissue broken up to carry out dedifferentiation by the specific combination of the various hormones in culture medium, thus inducing into callus, further inducing the individuality that makes new advances.This technology is widely used to agriculture and biological, medical research, has made huge contribution for micropropagation of plants, plant species protection and correlational study.
But owing to the culture medium that tissue culture uses containing substantial amounts of nutrient substance, not only it is suitable for plant cell and tissue growth, also being adapted for various antibacterial and fungus growth, the growth of antibacterial can consume the nutrient substance in culture medium in a large number, and the growth of plant cell is impacted simultaneously.Low additionally, due to Calli Differentiation degree, the defensive ability/resistance ability of antibacterial and fungus is poor, as easy as rolling off a log it is subject to antibacterial and material impact that fungus discharges, or poisoned, or produce variation, tissue culture's industry is caused strong influence, so totally aseptic environment is of crucial importance to tissue culture.
Group training room is the important means of control group training environment, but the room that mostly to be between common, current group training room, extraneous antibacterial, fungus etc. being unfavorable for when technical staff passes in and out, the material of group training environment takes group training indoor to, affects the effect of tissue culture unavoidably.
Summary of the invention
There are the problems referred to above based on prior art, the present invention provides a kind of double-deck group training room, and it includes mistress and Nei room, and mistress includes changing rooms, disinfection system, and interior room includes group training frame, detection system, temperature control system, group training light-source system, illuminator;Mistress's life-time service ultraviolet radiation sterilization, can first cushion when technical staff passes in and out in mistress, changes aseptic clothing and enters back into interior room, decreases the external world to a certain extent and is unfavorable for that the material of group training environment is brought to the probability that group training is indoor.A kind of double-deck group training room provided by the invention has that cost is low, sterilizing efficiency is high, the low density advantage of environmental bacteria.
A kind of double-deck group training room, it includes mistress and Nei room;
Mistress includes changing rooms, disinfection system, and interior room includes group training frame, detection system, temperature control system, group training light-source system, illuminator;
Group training frame is neatly placed in interior room, leaves passageway between each group of training frame;Detection system includes thermometer, is hung on the wall at four sides, interior room;Temperature control system includes air-conditioning, is placed in interior room;Group training light-source system includes white light light pipe, is arranged on each group of top training each layer of frame;Illuminator is arranged on group training ceiling portion;
Changing rooms is positioned at outer indoor, includes the public gown of a doctor after clothes hanger, sterilizing and medicated cap in changing rooms;Disinfection system includes ultraviolet light pipe, is arranged on mistress top;
Interior room is placed in mistress mutually, can enter interior room from mistress by the doorway of interior room.
Wherein, the doorframe of mistress has supply air system, towards drying outdoors, supply air system on doorframe outwards sprays filtrated air and the harmful substances such as dust floating in outside air, antibacterial and fungus can be stoped to a certain extent to enter into outer indoor, the harmful substances such as dust with entrance personnel can also be blown away, it is ensured that the environment of mistress cleans than external environment.
Wherein, the doorframe of interior room has supply air system, towards drying outdoors, supply air system on doorframe outwards sprays filtrated air and the harmful substances such as dust floating in mistress's air, antibacterial and fungus can be stoped to a certain extent to enter in interior room, the harmful substances such as dust with entrance personnel can also be blown away, it is ensured that the environment of interior room is than mistress clean environment.
Wherein, the doorway on interior room and mistress is built up with dislocation form, it is possible to stoping outside air directly to connect with interior room air, entering into from the external world have to through the buffering of mistress among interior room.
Wherein, having on the wall that interior room and mistress are separated by can the windowpane of filters ultra violet line, can be observed from the inside room of mistress by windowpane, windowpane makes technical staff can observe the situation of interior room from mistress, condition of at any time spy being oestrused makes strain, filter glass can stop the ultraviolet radiation of mistress in interior room simultaneously, causes plant tissue to make a variation.
Wherein, the group training light-source system in interior room is to train room power supply by timing power-on switch connection group, and the group training light source automatically controlling interior room is opened, and builds the alternation of light and darkness phenomenon in natural environment.
Wherein, interior room also has disinfection system, is arranged on the top of interior room, and whole group of training room is carried out sterilization treatment thoroughly by the disinfection system that can open interior ceiling portion after a collection of plant tissue culture terminates.
The invention has the beneficial effects as follows: using common tissue culture room as interior room, outside it, add one layer of mistress cushion, group training room is avoided directly to connect with the external world, reduce the external world to be suspended in the technical staff that dust in air, antibacterial and fungus etc. enter or passed in and out and be brought in group training room, affect group training environment;Technical staff more can change one's clothes in mistress, reduces the possibility being contaminated of pollution group training room further;Mistress opens disinfection system when not in use for a long time, makes mistress be constantly in aseptic or that bacterial density is extremely low state, it is possible to play a good cushioning effect for group training room, the probability that minimizing group training room is contaminated.
Accompanying drawing explanation
Fig. 1, the structural representation of a kind of double-deck group training room.
Wherein 1-mistress, room in 2-, 11-changing rooms, 12-disinfection system, 21-group training frame, 22-detects system, 23-temperature control system, 24-group training light-source system, 25-illuminator.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the invention will be further described.
A kind of double-deck group training room as shown in Figure 1, it includes mistress (1) and interior room (2);
Mistress (1) includes changing rooms (11), disinfection system (12), and interior room (2) include group training frame (21), detection system (22), temperature control system (23), group training light-source system (24), illuminator (25);
It is inner that group training frame (21) is neatly placed in interior room (2), leaves passageway between each group of training frame (21);Detection system (22) includes thermometer, is hung on the wall at four sides, interior room (2);Temperature control system (23) includes air-conditioning, is placed in interior room (2) inner;Group training light-source system (24) includes white light light pipe, is arranged on each group of top training each layer of frame (21);Illuminator (25) is arranged on group training ceiling portion;
Changing rooms (11) is positioned at mistress (1), includes the public gown of a doctor after clothes hanger, sterilizing and medicated cap in changing rooms (11);Disinfection system (12) includes ultraviolet light pipe, is arranged on mistress (1) top;
Interior room (2) is placed in mistress (1) mutually, room (2) in can being entered from mistress (1) by the doorway of interior room (2).
As the presently preferred embodiments, the doorframe of mistress (1) has supply air system, towards drying outdoors.
As the presently preferred embodiments, the doorframe of interior room (2) has supply air system, towards drying outdoors.
As the presently preferred embodiments, the doorway on interior room (2) and mistress (1) is built up with dislocation form.
As the presently preferred embodiments, the wall that interior room (2) and mistress (1) are separated by has can the windowpane of filters ultra violet line, can be observed from mistress (1) inwardly room (2) by windowpane.
As the presently preferred embodiments, group training light-source system (24) that interior room (2) are inner is to train room power supply by timing power-on switch connection group.
As the presently preferred embodiments, interior room (2) also have disinfection system (12), are arranged on the top of interior room (2).
As the presently preferred embodiments, group training room can be set by technical staff according to the condition of tissue culture of different plants, uses the air-conditioning of temperature control system (23) to arrange the temperature that group training is indoor, and the temperature making group training indoor is maintained at the optimum temperature of plant tissue growth.
As the presently preferred embodiments, group training room can be set by technical staff according to the condition of tissue culture of different plants, uses timing energization switch to arrange group training the opening and light of light source, group training lab simulation go out sooner or later substitute natural phenomena.
As the presently preferred embodiments, technical staff according to the condition of tissue culture of different plants, can control intensity of illumination by opening the light pipe organized in training light source of varying number, to reach to be best suitable for the intensity of illumination of plant tissue growth.
As the presently preferred embodiments, it is positioned in group training frame (21) by inoculating complete plant tissue culture bottle, corresponding temperature, intensity of illumination and light application time is set according to the condition needed for plant tissue culture, environment is trained according to setup control group in group training room, the upgrowth situation of observation of plant tissue in technical staff's entrance every day group training room.
Technical staff needs the disinfection system (12) that entrance group is trained during room in the outdoor room (1) of first closedown group training, subsequently into the outdoor room (1) of group training, when entering, the supply air system on outdoor room (1) doorframe of group training is constantly outwards blown, the materials such as the dust with technical staff, antibacterial are blown away and stop the float in outside air to enter, after entering mistress (1), changes the gown of a doctor of sterilizing, disposable slippers and medicated cap at changing rooms (11).
Group training indoor chamber (2) is entered back into after changing one's clothes, when entrance group trains indoor chamber (2), supply air system on interior room (2) doorframe is constantly outwards blown, the float in mistress (1) air is stoped to enter interior room (2) with technical staff, technical staff enters the upgrowth situation of interior room (2) observation of plant tissue afterwards, interior room (2) is left after observation, oneself clothes is changed mistress (1), it is then departed from mistress (1), after walking out mistress (1), reopens the disinfection system (12) of mistress (1).
Use a kind of double-deck group training room provided by the invention, it is possible to greatly reduce the bacterial density in the air that group training is indoor, considerably reduce plant tissue culture and be subject to the possibility of germ contamination, it will be apparent that reduce pollution rate.
Embodiment described above only have expressed one embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that, for the person of ordinary skill of the art, without departing from the inventive concept of the premise, it is also possible to making some deformation and improvement, these broadly fall into protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (7)

1. a double-deck group training room, it is characterised in that it includes mistress and Nei room;
Mistress includes changing rooms, disinfection system, and interior room includes group training frame, detection system, temperature control system, group training light-source system, illuminator;
Group training frame is neatly placed in interior room, leaves passageway between each group of training frame;Detection system includes thermometer, is hung on the wall at four sides, interior room;Temperature control system includes air-conditioning, is placed in interior room;Group training light-source system includes white light light pipe, is arranged on each group of top training each layer of frame;Illuminator is arranged on group training ceiling portion;
Changing rooms is positioned at outer indoor, includes the public gown of a doctor after clothes hanger, sterilizing and medicated cap in changing rooms;Disinfection system includes ultraviolet light pipe, is arranged on mistress top;
Interior room is placed in mistress mutually, can enter interior room from mistress by the doorway of interior room.
2. a kind of double-deck group training room according to claim 1, it is characterised in that on the doorframe of described mistress, there is supply air system, towards drying outdoors.
3. a kind of double-deck group training room according to claim 1, it is characterised in that on the doorframe of described interior room, there is supply air system, towards drying outdoors.
4. a kind of double-deck group training room according to claim 1, it is characterised in that the doorway on described interior room and mistress is built up with dislocation form.
5. a kind of double-deck group training room according to claim 1, it is characterised in that the wall that described interior room and mistress are separated by has can the windowpane of filters ultra violet line, can be observed from the inside room of mistress by windowpane.
6. a kind of double-deck group training room according to claim 1, it is characterised in that the group training light-source system in described interior room is to train room power supply by timing power-on switch connection group.
7. a kind of double-deck group training room according to claim 1, it is characterised in that described interior room also has disinfection system, is arranged on the top of interior room.
CN201610214289.6A 2016-04-08 2016-04-08 Double-layer tissue culture room Pending CN105794646A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610214289.6A CN105794646A (en) 2016-04-08 2016-04-08 Double-layer tissue culture room

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610214289.6A CN105794646A (en) 2016-04-08 2016-04-08 Double-layer tissue culture room

Publications (1)

Publication Number Publication Date
CN105794646A true CN105794646A (en) 2016-07-27

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102331067A (en) * 2011-05-24 2012-01-25 宁波奥克斯电气有限公司 Control method for protection of compressor during refrigeration cycle of direct-current frequency-conversion air conditioner
CN103135540A (en) * 2013-03-22 2013-06-05 河北大学 Plant tissue culture environmental information monitoring and simulating system
CN203732970U (en) * 2014-03-03 2014-07-23 上海邦伯现代农业技术有限公司 Tissue culture room intelligent monitor
CN203759515U (en) * 2014-03-03 2014-08-06 上海邦伯现代农业技术有限公司 Regulating and controlling and trace management system for tissue culture environment
CN104778686A (en) * 2015-03-23 2015-07-15 无锡中科智能农业发展有限责任公司 Tissue culture prewarning method and system based on scab monitoring
CN105371448A (en) * 2015-12-22 2016-03-02 南京科技职业学院 Constant temperature and constant humidity PLC control system for clean room

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102331067A (en) * 2011-05-24 2012-01-25 宁波奥克斯电气有限公司 Control method for protection of compressor during refrigeration cycle of direct-current frequency-conversion air conditioner
CN103135540A (en) * 2013-03-22 2013-06-05 河北大学 Plant tissue culture environmental information monitoring and simulating system
CN203732970U (en) * 2014-03-03 2014-07-23 上海邦伯现代农业技术有限公司 Tissue culture room intelligent monitor
CN203759515U (en) * 2014-03-03 2014-08-06 上海邦伯现代农业技术有限公司 Regulating and controlling and trace management system for tissue culture environment
CN104778686A (en) * 2015-03-23 2015-07-15 无锡中科智能农业发展有限责任公司 Tissue culture prewarning method and system based on scab monitoring
CN105371448A (en) * 2015-12-22 2016-03-02 南京科技职业学院 Constant temperature and constant humidity PLC control system for clean room

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Application publication date: 20160727