CN105794645A - Intelligent tissue culture room capable of analyzing growth state through internet - Google Patents
Intelligent tissue culture room capable of analyzing growth state through internet Download PDFInfo
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- CN105794645A CN105794645A CN201610214287.7A CN201610214287A CN105794645A CN 105794645 A CN105794645 A CN 105794645A CN 201610214287 A CN201610214287 A CN 201610214287A CN 105794645 A CN105794645 A CN 105794645A
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- 230000012010 growth Effects 0.000 title claims abstract description 43
- 238000004458 analytical method Methods 0.000 claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 17
- 238000001514 detection method Methods 0.000 claims description 8
- 241001074085 Scophthalmus aquosus Species 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000004378 air conditioning Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 2
- 239000010410 layer Substances 0.000 description 14
- 241000196324 Embryophyta Species 0.000 description 9
- 230000000844 anti-bacterial effect Effects 0.000 description 9
- 241000233866 Fungi Species 0.000 description 7
- 239000000428 dust Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 4
- 238000004161 plant tissue culture Methods 0.000 description 4
- 230000002354 daily effect Effects 0.000 description 3
- 238000007405 data analysis Methods 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 230000008467 tissue growth Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000032459 dedifferentiation Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B19/00—Programme-control systems
- G05B19/02—Programme-control systems electric
- G05B19/418—Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Automation & Control Theory (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Quality & Reliability (AREA)
- Manufacturing & Machinery (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides an intelligent tissue culture room capable of analyzing a growth state through the internet. The intelligent tissue culture room comprises an outer chamber, an inner chamber and a control system, wherein the inner chamber is provided with a growth state analysis system, the control system can be connected with an internet database, high-definition photos are taken, photo pixels are analyzed through network data and cloud computing, and the growth state of tissue culture seedlings is determined; local data and network data are analyzed through the control system, tissue culture standards are formed, the tissue culture room is uniformly controlled, and real-time, accurate and intelligent control can be realized. The provided intelligent tissue culture room capable of analyzing the growth state has the advantages of intelligence, low density of environment bacteria and capability of analyzing the growth state of the tissue culture seedlings.
Description
Technical field
The invention belongs to plant tissue culture apparatus field, particularly relate to a kind of the Internet and analyze the smart group training room of growth conditions.
Background technology
Tissue culture technique is to be placed in culture medium by work organ, tissue or cell under sterile conditions, and it is placed in suitable environment, carry out the cell of cultivation, tissue or individuality continuously, it mainly utilizes cellular omnipotency, namely each cell has the hereditary material of complete set, inducing the plant tissue broken up to carry out dedifferentiation by the specific combination of the various hormones in culture medium, thus inducing into callus, further inducing the individuality that makes new advances.This technology is widely used to agriculture and biological, medical research, has made huge contribution for micropropagation of plants, plant species protection and correlational study.
But owing to the culture medium that tissue culture uses containing substantial amounts of nutrient substance, not only it is suitable for plant cell and tissue growth, also being adapted for various antibacterial and fungus growth, the growth of antibacterial can consume the nutrient substance in culture medium in a large number, and the growth of plant cell is impacted simultaneously.Low additionally, due to Calli Differentiation degree, the defensive ability/resistance ability of antibacterial and fungus is poor, as easy as rolling off a log it is subject to antibacterial and material impact that fungus discharges, or poisoned, or produce variation, tissue culture's industry is caused strong influence, so totally aseptic environment is of crucial importance to tissue culture.
Group training room is the important means of control group training environment, but the room that mostly to be between common, current group training room, unavoidably can by extraneous antibacterial when technical staff passes in and out, funguses etc. are unfavorable for that the material of group training environment takes group training indoor to, affect the effect of tissue culture, although there is temperature additionally current group training room, the enviromental monitoring equipments such as intensity of illumination, but lack the detection system of tissue cultured seedling growth conditions, need nonetheless remain for the growth conditions of manual observations's tissue cultured seedling, intelligent group training room truly can not be realized, the growth conditions simultaneously analyzing tissue cultured seedling needs googol according to amount and complicated calculating.
Summary of the invention
There are the problems referred to above based on prior art, the present invention provides the smart group training room that growth conditions is analyzed in a kind of the Internet, it includes mistress, interior room and control system, mistress includes changing rooms, disinfection system, interior room includes group training frame, detection system, temperature control system, group training light-source system, illuminator, growth conditions analysis system, and control system includes PLC control system and host computer;Mistress's life-time service ultraviolet radiation sterilization, can first cushion in mistress when technical staff passes in and out, change aseptic clothing and enter back into interior room, decrease the external world to a certain extent and be unfavorable for that the material of group training environment is brought to the probability that group training is indoor, simultaneously by organizing the position replacement of the training every interlayer of frame, technical staff can easily manage the tissue cultured seedling of eminence, spatial altitude can be made full use of and save space, can also pass through to shoot high definition photo, utilize network data and cloud computing to analyze photograph pixel and determine the growth conditions of tissue cultured seedling;Group training room utilizes control system analysis local data and network data, formation group training standard, then group training room is carried out unified control can accomplish real-time, accurate and intelligentized control.A kind of the Internet provided by the invention is analyzed the smart group training room of growth conditions and is had the advantage that intellectuality, environmental bacteria density are low and can analyze tissue cultured seedling growth conditions.
The smart group training room of growth conditions is analyzed in a kind of the Internet, and it includes mistress, interior room and control system;
Mistress includes changing rooms, disinfection system, and interior room includes group training frame, detection system, temperature control system, group training light-source system, illuminator, and control system includes PLC control system and host computer;
Group training frame is neatly placed in interior room, leaves passageway between each group of training frame, and group every layer of frame of training can be moved and switch along track by gear driven;Detection system includes temperature sensor and intensity of illumination sensor, is hung on the wall at four sides, interior room;Temperature control system includes air-conditioning, is placed in interior room;Group training light-source system includes white light light pipe, is arranged on each group of top training each layer of frame;Illuminator is arranged on group training ceiling portion, and growth conditions is analyzed system and included the high-definition camera being arranged on bottom group training rack-layer and the analysis software deposited in control system;
Changing rooms is positioned at outer indoor, includes the public gown of a doctor after clothes hanger, sterilizing and medicated cap in changing rooms;Disinfection system includes ultraviolet light pipe, is arranged on mistress top;
Interior room is placed in mistress mutually, can enter interior room from mistress by the doorway of interior room, and control system is positioned at outer indoor, is connected with other system and network data base by the Internet, controls to coordinate each system and runs.
Wherein, the doorframe of mistress has supply air system, towards drying outdoors, supply air system on doorframe outwards sprays filtrated air and the harmful substances such as dust floating in outside air, antibacterial and fungus can be stoped to a certain extent to enter into outer indoor, the harmful substances such as dust with entrance personnel can also be blown away, it is ensured that the environment of mistress cleans than external environment.
Wherein, the doorframe of interior room has supply air system, towards drying outdoors, supply air system on doorframe outwards sprays filtrated air and the harmful substances such as dust floating in mistress's air, antibacterial and fungus can be stoped to a certain extent to enter in interior room, the harmful substances such as dust with entrance personnel can also be blown away, it is ensured that the environment of interior room is than mistress clean environment.
Wherein, the doorway on interior room and mistress is built up with dislocation form, it is possible to stoping outside air directly to connect with interior room air, entering into from the external world have to through the buffering of mistress among interior room.
Wherein, having on the wall that interior room and mistress are separated by can the windowpane of filters ultra violet line, can be observed from the inside room of mistress by windowpane, windowpane makes technical staff can observe the situation of interior room from mistress, condition of at any time spy being oestrused makes strain, filter glass can stop the ultraviolet radiation of mistress in interior room simultaneously, causes plant tissue to make a variation.
Wherein, the group training light-source system in interior room is to train room power supply by timing power-on switch connection group, and the group training light source automatically controlling interior room is opened, and builds the alternation of light and darkness phenomenon in natural environment.
Wherein, interior room also has disinfection system, is arranged on the top of interior room, and whole group of training room is carried out sterilization treatment thoroughly by the disinfection system that can open interior ceiling portion after a collection of plant tissue culture terminates.
Wherein, group in interior room training frame is connected with motor, can be driven by motor to organize and carries out place-exchange between the different layers trained on frame, makes the tissue cultured seedling being placed in eminence can move to lower, facilitates technical staff's daily management.
Wherein, the host computer in control system is first connected with PLC control system and network data base, and PLC control system is connected with the indoor other system of group training again.
Wherein, the host computer in control system is analyzed contrast, formation group training standard according to local data and network data, according to standard, PLC control system is made adjustment, and then controls the operation of whole group of training room.
Wherein, it is shoot tissue culture bottle by high-definition camera that growth conditions analyzes system, by analyzing software, captured photo is carried out pixel analysis, it is determined that the upgrowth situation of tissue cultured seedling.
The invention has the beneficial effects as follows: using common tissue culture room as interior room, outside it, add one layer of mistress cushion, group training room is avoided directly to connect with the external world, reduce the external world to be suspended in the technical staff that dust in air, antibacterial and fungus etc. enter or passed in and out and be brought in group training room, affect group training environment;Technical staff more can change one's clothes in mistress, reduces the possibility being contaminated of pollution group training room further;Mistress opens disinfection system when not in use for a long time, makes mistress be constantly in aseptic or that bacterial density is extremely low state, it is possible to play a good cushioning effect for group training room, the probability that minimizing group training room is contaminated;Between utilization group every layer of frame of training can switch and the tissue cultured seedling of eminence is moved to lower, facilitate daily management, make full use of spatial altitude and the area of saving group training room, reach space-saving purpose;Use growth conditions to analyze system and tissue cultured seedling photo is carried out pixel analysis, it may be determined that the growth conditions of tissue cultured seedling;Use control system analysis local data and network data, formation group training standard, then group training room is carried out unified control can accomplish real-time, accurate and intelligentized control.
Accompanying drawing explanation
Fig. 1, the structural representation of the smart group training room of growth conditions is analyzed in a kind of the Internet.
Fig. 2, the side structure schematic diagram of the group training frame of the smart group training room of growth conditions is analyzed in a kind of the Internet.
Fig. 3, this links schematic diagram to control system and other system in the smart group training room of a kind of the Internet analysis growth conditions.
Wherein 1-mistress, room in 2-, 3-controls system, 11-changing rooms, 12-disinfection system, 21-group training frame, 22-detects system, 23-temperature control system, 24-group training light-source system, 25-illuminator, 211-gear, 212-track, 261-high-definition camera, 31-PLC controls system, 32-host computer.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the invention will be further described.
The smart group training room of growth conditions is analyzed in a kind of the Internet as shown in Figure 1, and it includes mistress (1), interior room (2) and controls system (3);
Mistress (1) includes changing rooms (11), disinfection system (12), interior room (2) includes group training frame (21), detection system (22), temperature control system (23), group training light-source system (24), illuminator (25), controls system (3) and includes PLC control system (31) and host computer (32);
Neatly to be placed in interior room (2) inner for group training frame (21), leaves passageway between each group of training frame (21), and group every layer of frame of training can pass through gear (211) and drive and move and switch along track (212);Detection system (22) includes temperature sensor and intensity of illumination sensor, is hung on the wall at four sides, interior room (2);Temperature control system (23) includes air-conditioning, is placed in interior room (2) inner;Group training light-source system (24) includes white light light pipe, is arranged on each group of top training each layer of frame (21);Illuminator (25) is arranged on group training ceiling portion, and growth conditions is analyzed system and included the high-definition camera (261) being arranged on bottom group training rack-layer and the analysis software deposited in control system (3);
Changing rooms (11) is positioned at mistress (1), includes the public gown of a doctor after clothes hanger, sterilizing and medicated cap in changing rooms (11);Disinfection system (12) includes ultraviolet light pipe, is arranged on mistress (1) top;
Interior room (2) is placed in mistress (1) mutually, room (2) in can being entered from mistress (1) by the doorway of interior room (2), controls system (3) and is positioned at mistress (1), is connected with other system and network data base by the Internet, controls to coordinate each system and runs.
As the presently preferred embodiments, the doorframe of mistress (1) has supply air system, towards drying outdoors.
As the presently preferred embodiments, the doorframe of interior room (2) has supply air system, towards drying outdoors.
As the presently preferred embodiments, the doorway on interior room (2) and mistress (1) is built up with dislocation form.
As the presently preferred embodiments, the wall that interior room (2) and mistress (1) are separated by has can the windowpane of filters ultra violet line, can be observed from mistress (1) inwardly room (2) by windowpane.
As the presently preferred embodiments, group training light-source system (24) that interior room (2) are inner is to train room power supply by timing power-on switch connection group.
As the presently preferred embodiments, interior room (2) also have disinfection system (12), are arranged on the top of interior room (2).
As the presently preferred embodiments, group in interior room training frame is connected with motor, can be driven by motor to organize and carries out place-exchange between the different layers trained on frame (21).
As the presently preferred embodiments, controlling the host computer (32) in system (3) and be first connected with PLC control system (31) and network data base connection, PLC control system (31) is connected with the indoor other system of group training again.
As the presently preferred embodiments, the host computer (32) controlled in system (3) is analyzed contrast, formation group training standard according to local data and network data, according to standard, PLC control system (31) is made adjustment, and then controls the operation of whole group of training room.
As the presently preferred embodiments, as the presently preferred embodiments, host computer (32) makes group training standard according to local data and network data analysis, instruction is sent to PLC control system (32), group training room is set, the air-conditioning using temperature control system (23) arranges the temperature that group training is indoor, and the temperature making group training indoor is maintained at the optimum temperature of plant tissue growth.
As the presently preferred embodiments, skill host computer (32) makes group training standard according to local data and network data analysis, instruction is sent to PLC control system (32), group training room is set, use timing energization switch that group training the opening and light of light source is set, group training lab simulation go out sooner or later substitute natural phenomena.
As the presently preferred embodiments, host computer (32) makes group training standard according to local data and network data analysis, instruction is sent to PLC control system (32), group training room is set, by opening the light pipe control intensity of illumination that the group of varying number is trained in light source, to reach to be best suitable for the intensity of illumination of plant tissue growth.
As the presently preferred embodiments, it is positioned in group training frame (21) by inoculating complete plant tissue culture bottle, host computer (32) in control system (3) is according to the group training standard analyzed, PLC control system (31) is sent instruction, control group training environment, the upgrowth situation of observation of plant tissue in technical staff's entrance every day group training room.
As the presently preferred embodiments, place and when inoculating complete tissue culture bottle, can first occupy following group training rack-layer, then use motor by upper and lower group training rack-layer switch, then place tissue culture bottle;The equally possible tissue cultured seedling first having processed low layer when tissue cultured seedling is carried out daily management, the position exchanging levels group training rack-layer again through motor continues with remaining tissue cultured seedling.
As the presently preferred embodiments, tissue cultured seedling is once shot by high-definition camera (261) for every 12 hours, photo is transferred to control system (3,), utilized network data base and cloud computing that photo is carried out pixel analysis by the analysis software in host computer (32), it is determined that whether tissue cultured seedling contaminated and its growth conditions.
Technical staff needs the disinfection system (12) that entrance group is trained during room in the outdoor room (1) of first closedown group training, subsequently into the outdoor room (1) of group training, when entering, the supply air system on outdoor room (1) doorframe of group training is constantly outwards blown, the materials such as the dust with technical staff, antibacterial are blown away and stop the float in outside air to enter, after entering mistress (1), changes the gown of a doctor of sterilizing, disposable slippers and medicated cap at changing rooms (11).
Group training indoor chamber (2) is entered back into after changing one's clothes, when entrance group trains indoor chamber (2), supply air system on interior room (2) doorframe is constantly outwards blown, the float in mistress (1) air is stoped to enter interior room (2) with technical staff, technical staff enters the upgrowth situation of interior room (2) observation of plant tissue afterwards, interior room (2) is left after observation, oneself clothes is changed mistress (1), it is then departed from mistress (1), after walking out mistress (1), reopens the disinfection system (12) of mistress (1).
A kind of the Internet provided by the invention is used to analyze the smart group training room of growth conditions, the bacterial density in the air that group training is indoor can be greatly reduced, considerably reduce plant tissue culture and be subject to the possibility of germ contamination, significantly reduce pollution rate, take full advantage of the eminence space of group training room simultaneously, save the area of group training room, intelligent, accurate, real-time control can have been realized and analyze tissue cultured seedling upgrowth situation, improve the quality of tissue culture.
Embodiment described above only have expressed one embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that, for the person of ordinary skill of the art, without departing from the inventive concept of the premise, it is also possible to making some deformation and improvement, these broadly fall into protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. the smart group training room of a Internet analysis growth conditions, it is characterised in that it includes mistress, interior room and control system;
Mistress includes changing rooms, disinfection system, and interior room includes group training frame, detection system, temperature control system, group training light-source system, illuminator, growth conditions analysis system, and control system includes PLC control system and host computer;
Group training frame is neatly placed in interior room, leaves passageway between each group of training frame, and group every layer of frame of training can be moved and switch along track by gear driven;Detection system includes temperature sensor and intensity of illumination sensor, is hung on the wall at four sides, interior room;Temperature control system includes air-conditioning, is placed in interior room;Group training light-source system includes white light light pipe, is arranged on each group of top training each layer of frame;Illuminator is arranged on group training ceiling portion;Growth conditions is analyzed system and is included the high-definition camera being arranged on bottom group training rack-layer and the analysis software deposited in control system;
Changing rooms is positioned at outer indoor, includes the public gown of a doctor after clothes hanger, sterilizing and medicated cap in changing rooms;Disinfection system includes ultraviolet light pipe, is arranged on mistress top;
Interior room is placed in mistress mutually, can enter interior room from mistress by the doorway of interior room, and control system is positioned at outer indoor, is connected with other system and network data base by the Internet, controls to coordinate each system and runs.
2. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterised in that have supply air system on the doorframe of described mistress and Nei room, towards drying outdoors.
3. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterised in that the doorway on described interior room and mistress is built up with dislocation form.
4. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterised in that the wall that described interior room and mistress are separated by has can the windowpane of filters ultra violet line, can be observed from the inside room of mistress by windowpane.
5. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterised in that the group training light-source system in described interior room is to train room power supply by timing power-on switch connection group.
6. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterised in that described interior room also has disinfection system, is arranged on the top of interior room.
7. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterised in that the group training frame in described interior room is connected with motor, can be driven by motor to organize and carries out place-exchange between the different layers trained on frame.
8. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterised in that the host computer in described control system is first connected with PLC control system and network data base, and PLC control system is connected with the indoor other system of group training again.
9. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterized in that, the described host computer in control system is analyzed contrast according to local data and network data, formation group training standard, according to standard, PLC control system is made adjustment, and then control the operation of whole group of training room.
10. the smart group training room of growth conditions is analyzed in a kind of the Internet according to claim 1, it is characterized in that, it is shoot tissue culture bottle by high-definition camera that described growth conditions analyzes system, by analyzing software, captured photo is carried out pixel analysis, it is determined that the upgrowth situation of tissue cultured seedling.
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CN106886857A (en) * | 2017-02-23 | 2017-06-23 | 佛山市融信通企业咨询服务有限公司 | A kind of intelligent flower culture person's performance appraisal system |
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