CN105794444A - Germination method for climbing fern spores - Google Patents
Germination method for climbing fern spores Download PDFInfo
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- CN105794444A CN105794444A CN201410848056.2A CN201410848056A CN105794444A CN 105794444 A CN105794444 A CN 105794444A CN 201410848056 A CN201410848056 A CN 201410848056A CN 105794444 A CN105794444 A CN 105794444A
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Abstract
The invention relates to a germination method for climbing fern spores. The method comprises the steps: (1) gathering spores of climbing fern plants for later use; (2) loading garden soil to the lower part of a flowerpot, drenching the garden soil with carbendazol or topsin liquid, covering the soil with a thin film, carrying out sealing for 1 to 2 days, laying a high-temperature disinfected peat soil matrix with the thickness of 2cm to 3cm, and drenching the matrix with water; (3) sowing the stand-by spores to the surface layer of the peat soil matrix according to the density of 0.25g/m<2> to 0.375g/m<2>; (4) carrying out greenhouse culturing in a manner of covering with glass and maintaining the temperature of 20 DEG C to 30 DEG C, the illumination intensity of 2,000lx to 3,000lx and the illumination time of 12h/d, or carrying out covering with a shading screen with the shading degree of 75% and keeping the soil humidity of 60% to 88% and the air humidity of 80% to 90%; (5) carrying out fertilizing 30 days after sowing; and (6) carrying out transplanting when the number of accessory pinnae grown from each juvenile sporophyte is 2 to 3 and the height of each plant is 8cm to 14cm. According to the method, the germinating rate is high, the rotting rate is low, the seedling rate is high, and the transplanting survival rate of juvenile sporophytes reaches 90% or more.
Description
Technical field
The present invention relates to a kind of method for propagation, particularly relate to the germination method of a kind of Spora Lygodii spore.
Background technology
Spora Lygodii is common pteridophyta, it belongs to Pteridophyta, thin capsule Filicopsida, straight Herba pteridii latiusculi order, Lygodiaceae, Lygodium, it is distributed in subtropical and tropical zones, north is to Shanxi and Southern Henan, and west reaches Sichuan, Yunnan and Guizhou, and herb or ripe spore can hyoscines, slightly sweet flavor, cold in nature, there is heat-clearing and toxic substances removing, effect of relieving stranguria by diuresis；Plant tool is wound around habit, is the good material of vertical greening, and its ingredient is complex, has bioactive ingredients, mainly by phenolic compound, flavonoid, alkaloids, steroidal and triterpenoid compound etc..Test shows: the ethanol extraction of Spora Lygodii spore 50% has significant antiandrogen effect, Resina garciniae coccus, staphylococcus aureus, bacillus subtilis and beta hemolytic streptococcus etc. are all had good fungistatic effect by Spora Lygodii ethanol extract, moreover, it has been found that Spora Lygodii has the effect of resisiting influenza virus, HIV-1 virus, sindbis alphavirus etc..In the book such as " good book on Chinese herbal medicine ", " book on Chinese herbal medicine justice ", " Chinese Plants will ", " Guangxi Chinese medicinal herbal ", " Hunan medicine will ", " conventional Chinese herbal medicine handbook ", " Hunan medicine will ", " Chinese Plants will " all on the books.Can be used for controlling the diseases such as urinary tract infection, hepatitis, oedema due to nephritis, lithangiuria, nebulousurine, leucorrhea, mumps, laryngopharynx swelling and pain, enteritis, dysentery, herpes zoster, skin eczema.
Spora Lygodii is conventional Chinese medicine, and consumption is very big, and its crude drug is all be main by purchasing natural crude drugs.At present owing to artificial a large amount of collections and soil are excessively developed the resource causing Spora Lygodii and day by day reduced.Therefore the artificial growth carrying out Spora Lygodii is a kind of effective measures of protection Spora Lygodii germ plasm resource.
In vascular plant, the monoid that pteridophyta is unique sporinite and gametocyte can be lived on one's own life.The spore that most pteridophyta spore bodies produce can sprout the gametocyte for living on one's own life, and then forms new sporinite by spermatiation.Under natural conditions, pteridophyta spore is typically via illumination breaking dormancy, relying on action of gravity complete Germinal Vesicle Migration and set up polarity, then pass through twice asymmetric cell division and produce rhizoid and protonema germinal cell respectively, protonema germinal cell continues division and forms gametocyte.Visible, spore germination is the key link in pteridophyta life cycle.
" foundation of Spora Lygodii sporinite rapid propagation system " [Zeng Zhi, Jiang Wei etc. the foundation [J] of Spora Lygodii sporinite rapid propagation system. Hubei Institute For Nationalities's journal (natural science edition), 2012,30 (3): 335-338] study on reproduction has been carried out with Spora Lygodii sporophyll for material, obtaining sporophyll, to be divided into the optimum condition of GGB be MS+NAA0.3mg/L+6-BA0.5mg/L, and inductivity reaches 90%；It is MS+6-BA1.5mg/+NaH that GGB proliferation and differentiation forms the optimum condition of seedling2PO4200mg/L, growth coefficient reaches 7.8；Root media optimum condition is 1/2MS+NAA0.1mg/L+2% sucrose, rooting rate reach 86.7% or 1/2MS+IBA0.2mg/L+2% sucrose rooting rate be 87%；Acclimatization and transplants is to perlite: in the substrate of the peat composed of rotten mosses=2:1, survival rate 87.5%.A large amount of Spora Lygodii tissue cultured seedling can be produced in a short time, provide foundation for industrial seedling rearing.But said method is with Spora Lygodii sporophyll for material, to such as how spore be material so that it is fast-germination becomes gametocyte, sporinite and does not provide the enlightenment of any technology.
CN103999750A discloses the propagation method of a kind of Herba Botrychii spore, including following four step: one, Herba Botrychii spore collect, pretreatment, two, Herba Botrychii Spore cultivation benchmark standby, process, three, spore sowing and management, four, the little transplantation of seedlings of Herba Botrychii.Due to the fact that and have employed above-mentioned technical scheme, solve conventional Herba Botrychii seedling and be completely dependent on the limitation of wild resource, and tradition Propagtion of Pteridophyta Plants tissue culture invests big, to need professional etc. technological deficiency problem.There is the advantages such as breeding coefficient height, cost is low, planting percent is high, rotting rate is low, it is possible to few female parent material will be utilized to be trained high-quality seedling to greatest extent, as planting of maternal, be one of optimal path breeding Herba Botrychii at present.But said method is not particularly suited for the breeding of Spora Lygodii spore.
In view of this, the special proposition present invention.
Summary of the invention
It is an object of the invention to provide the germination method of a kind of Spora Lygodii spore, the method germination rate is high, rotting rate is low, planting percent is high.
For achieving the above object, the present invention adopts the following technical scheme that
The germination method of a kind of Spora Lygodii spore, comprises the steps:
1) collect seed
Gather Spora Lygodii plant spore, dress envelope to be saved in constant temperature refrigerator standby；
2) prepare before sowing
Bottom flowerpot, fill garden mould, drench carbendazim or thiophanate liquid, cover transparent membrane and seal 1～2 day, then spread 2～3cm thick, through the peat soil substrate of high-temperature sterilization, water of drenching；
3) sowing
By step 1) standby spore presses 0.25g～0.375g/m2Thickness of sowing be seeded in peat soil substrate top layer；
4) daily management
Cover glass, maintain temperature 20～30 DEG C, intensity of illumination is 2000～3000lx, light application time be 12h/d or cover shade rate be the shade net of 75% to control intensity of illumination, and keep soil moisture 60%～88%, air humidity be 80%～90% greenhouse in cultivate；
5) strong sprout
Within 30th day, proceed by fertilising in sowing, within every 10 days, apply fertilizer 1 time；
6) transplant for the first time
It is transplanted to nursery in equipped with the nutrient cup of seedling medium when juvenile sporophyte grows 2～3 accessory pinnas, plant 8～14cm height.
Spore germination is the key link in pteridophyta life cycle.How improving the germination rate of spore, reducing rotting rate thus improving planting percent, prior art does not provide more enlightenment, Spora Lygodii spore is being carried out substantial amounts of research by the present invention, substantial amounts of research has been carried out from the suitableeest Spora Lygodii spore germination temperature, moisture, substrate, shade rate, thickness of sowing, transplanting opportunity etc., draw the above-mentioned germination method of the present invention, the inventive method matrix composition is simple, operational approach is simple, solve the technical bottleneck in Spora Lygodii sporogenesis process so that it is juvenile sporophyte transplanting survival rate reaches more than 90%.The application of this technology is by the foundation strong for the breeding offer of Spora Lygodii spore land for growing field crops, great popularization.
In germination method of the present invention, wherein, described step 1) be: the acquisition time of spore is 8～JIUYUE in summer, gathering the Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, after 5～7d, Sporangium splits, spore is scattered naturally, being collected by spore, seal up for safekeeping with sealing is packed, dress seals and is saved in the constant temperature refrigerator of 5 ± 1 DEG C standby.
Further, step 2) in, at the garden mould of built-in 4/5 volume of flowerpot, its top layer is twisted with the fingers thin with hands；Described peat soil substrate was 80 mesh sieves the peat soil substrate through 120 DEG C of high-temperature sterilizations.
Further, step 2) in, described water of drenching is adopt the mode of spray painting to drench water.
In above-mentioned germination method, step 3) in, described sowing is seeded in peat soil substrate top layer equably for being stained with spore powder with clean brush pen.
In above-mentioned germination method, step 5) in, described fertilising adopts spray painting, the thin mode executed to apply fertilizer.
In above-mentioned germination method, step 6) in, the mixed-matrix that described seedling medium is garden mould and peat soil forms with the mass ratio of 3:1.
Further, carrying out step 6) described in transplanting time drench and determine root water.
After planting, after Spora Lygodii gametocyte is formed, open the glass ventilation 1～2h covering on flowerpot every day and spray 1 time.
Step 2) in, the sowing time of described sowing is mid-September in autumn, selects the flowerpot nursery of high 25cm, diameter 30cm in booth.
Matrix composition of the present invention is simple, and operational approach is simple, solves the technical bottleneck in Spora Lygodii sporogenesis process so that it is juvenile sporophyte transplanting survival rate reaches more than 90%.The application of this technology is by the foundation strong for the breeding offer of Spora Lygodii spore land for growing field crops, great popularization.
Compared with prior art, the advantage that the germination method of Spora Lygodii spore provided by the present invention has germination rate height, rotting rate is low, planting percent is high.
Detailed description of the invention
Being below the specific embodiment of the present invention, described embodiment is to further describe the present invention, rather than the restriction present invention.
Embodiment 1, Spora Lygodii spore germination method
1) collect seed
The acquisition time of spore is 8～JIUYUE in summer, gathers the Spora Lygodii plant with maturation spore, is then placed on thin film and naturally dries in the shade, after 5～7d, Sporangium splits, and spore is scattered naturally, is collected by spore, sealing up for safekeeping with sealing is packed, dress seals and is saved in the constant temperature refrigerator of 5 ± 1 DEG C standby；
2) prepare before sowing
Sowing time is mid-September in autumn, the flowerpot nursery of high 25cm, diameter 30cm is selected in booth, garden mould at basin built-in 4/5, drench carbendazim, cover transparent membrane to seal 2 days, then spread that 2～3cm is thick, cross 80 order dusting covers and the peat soil substrate through 120 DEG C of high-temperature sterilizations, adopt the mode of spray painting to drench water；
3) sowing
With the spore gathered then by 0.25g/m2Sowing, is stained with spore powder with clean brush pen during sowing and is seeded in peat soil substrate top layer uniformly；
4) daily management
Cover glass, maintain temperature 20 DEG C, intensity of illumination be 2000lx, light application time be 12h/d, keep soil moisture 60%, air humidity be 80% greenhouse in cultivate；
5) strong sprout
Sow the 30th day and proceed by fertilising, 10 days/1 time.
6) transplant for the first time
Being transplanted in nutrient cup when juvenile sporophyte grows 2～3 accessory pinnas, plant 8～14cm height nursery, seedling medium is garden mould: the mixed-matrix of peat soil=3:1, need to drench and determine root water during transplanting.
Embodiment 2, Spora Lygodii spore germination method
1) collect seed
The acquisition time of spore is 8～JIUYUE in summer, gathers the Spora Lygodii plant with maturation spore, is then placed on thin film and naturally dries in the shade, after 5～7d, Sporangium splits, and spore is scattered naturally, is collected by spore, sealing up for safekeeping with sealing is packed, dress seals and is saved in the constant temperature refrigerator of 5 ± 1 DEG C standby；
2) prepare before sowing
Sowing time is mid-September in autumn, the flowerpot nursery of high 25cm, diameter 30cm is selected in booth, garden mould at basin built-in 4/5, drench thiophanate liquid (by specification), cover transparent membrane to seal 1 day, then spread that 2～3cm is thick, cross 80 order dusting covers and the peat soil substrate through 120 DEG C of high-temperature sterilizations, adopt the mode of spray painting to drench water；
3) sowing
With the spore gathered then by 0.375g/m2Sowing, is stained with spore powder with clean brush pen during sowing and is seeded in peat soil substrate top layer uniformly；
4) daily management
Cover glass, maintain temperature 30 DEG C, intensity of illumination be 3000lx, light application time be 12h/d, keep soil moisture 88%, air humidity be 90% greenhouse in cultivate；
5) strong sprout
Sow the 30th day and proceed by fertilising, 10 days/1 time.
6) transplant for the first time
Being transplanted in nutrient cup when juvenile sporophyte grows 2～3 accessory pinnas, plant 8～14cm height nursery, seedling medium is garden mould: the mixed-matrix of peat soil=3:1, need to drench and determine root water during transplanting.
Embodiment 3, Spora Lygodii spore germination method
1) collect seed
The acquisition time of spore is 8～JIUYUE in summer, gathers the Spora Lygodii plant with maturation spore, is then placed on thin film and naturally dries in the shade, after 5～7d, Sporangium splits, and spore is scattered naturally, is collected by spore, sealing up for safekeeping with sealing is packed, dress seals and is saved in the constant temperature refrigerator of 5 ± 1 DEG C standby；
2) prepare before sowing
Sowing time is mid-September in autumn, selects the flowerpot nursery of high 25cm, diameter 30cm in booth, at the garden mould of basin built-in 4/5, drench carbendazim, cover transparent membrane to seal 1～2 day, then spread 2～3cm thickness, cross 80 order dusting covers and the peat soil substrate through 120 DEG C of high-temperature sterilizations, water of drenching；
3) sowing
With the spore gathered then by 0.50g/m2Sowing, is stained with spore powder with clean brush pen during sowing and is seeded in peat soil substrate top layer uniformly；After planting, after Spora Lygodii gametocyte is formed, open the glass ventilation 2h covered on flowerpot every day and spray 1 time.
4) daily management
Cover glass, cover shade rate be the shade net of 75% to control intensity of illumination, keep soil moisture 75%, air humidity be 85% greenhouse in cultivate；
5) strong sprout
Sow the 30th day and proceed by fertilising, 10 days/1 time.
6) transplant for the first time
Being transplanted in nutrient cup when juvenile sporophyte grows 2～3 accessory pinnas, plant 8～14cm height nursery, seedling medium is garden mould: the mixed-matrix of peat soil=3:1.
Embodiment 4, Spora Lygodii spore germination method
1) collect seed
The acquisition time of spore is 8～JIUYUE in summer, gathers the Spora Lygodii plant with maturation spore, is then placed on thin film and naturally dries in the shade, after 5～7d, Sporangium splits, and spore is scattered naturally, is collected by spore, sealing up for safekeeping with sealing is packed, dress seals and is saved in the constant temperature refrigerator of 5 ± 1 DEG C standby；
2) prepare before sowing
Sowing time is mid-September in autumn, the flowerpot nursery of high 25cm, diameter 30cm is selected in booth, garden mould at basin built-in 4/5, drench thiophanate liquid (by specification), cover transparent membrane to seal 2 days, then spread 2～3cm thickness, cross 80 order dusting covers and the peat soil substrate through 120 DEG C of high-temperature sterilizations, water of drenching；
3) sowing
With the spore gathered then by 0.30g/m2Sowing, is stained with spore powder with clean brush pen during sowing and is seeded in peat soil substrate top layer uniformly；After planting, after Spora Lygodii gametocyte is formed, open the glass ventilation 1h covered on flowerpot every day and spray 1 time.
4) daily management
Cover glass, maintain temperature 20～30 DEG C, intensity of illumination is 2000～3000lx, light application time is 12h/d or to cover shade rate be that the shade net of 75% is to control intensity of illumination, keep soil moisture 60%～88%, air humidity be 80%～90% greenhouse in cultivate；
5) strong sprout
Sow the 30th day and begin with spray painting, the thin mode executed executes complete good good fortune fertilizer, 10 days/1 time.
6) transplant for the first time
Being transplanted in nutrient cup when juvenile sporophyte grows 2～3 accessory pinnas, plant 8～14cm height nursery, seedling medium is garden mould: the mixed-matrix of peat soil=3:1.
Test example 1, the suitableeest Spora Lygodii spore germination temperature test
Gathering the wild Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, treat that Sporangium splits after 5～7d, spore is scattered naturally, is collected by the spore on thin film, standby with sealing in the packed constant temperature refrigerator sealing and being saved in 5 DEG C up for safekeeping；
Temperature experiment
Being set to constant temperature, each process repeats 3 times；Periodically illumination is 3000lx；Time is in 12h/d artificial culture case respectively 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, gives constant temperature periodically illumination and completely black 8 process of constant temperature.First in culture dish, spread two layers of filter paper during test, and infiltrate filter paper with distilled water, then the spore of Spora Lygodii is sprinkling upon on filter paper equably, be then placed in corresponding artificial culture case, regularly utilize microscopic.
Result
Surveying to the 30th day that germination rate is known to be begun to sprout when the periodically illumination of 30 DEG C of constant temperature on the 8th day, germination percentage reaches 76.7%；Within 13rd day, starting to sprout under constant temperature 25 DEG C periodically illumination condition, germination percentage is 68.2%；Within 20th day, starting to sprout under constant temperature 20 DEG C periodically illumination condition, germination percentage is 19.6%；Within 15th day, starting to sprout under constant temperature 35 DEG C periodically illumination condition, germination percentage is only 1.5%；Best under constant temperature, completely black condition one group is 30 DEG C, also 20 talentes must see its spore germination.Visible Spora Lygodii spore is under identical temperature conditions, and under constant temperature periodically illumination condition, spore germination is superior to the spore germination under the completely black condition of constant temperature.Still must the illumination of some strength in visible spore germination process.Visible Spora Lygodii spore is best suitable for sprouting under 30 DEG C of constant temperature periodically illumination condition.
Test example 2, the suitableeest Spora Lygodii spore germination moisture test
Gathering the wild Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, when Sporangium splits after 5～7d, spore is scattered naturally, is collected by the spore on thin film, standby with sealing in the packed constant temperature refrigerator sealing and being saved in 5 DEG C up for safekeeping；
Moisture test
It is 3000lx, time 12h/d in periodicity illumination.Temperature is cultivate in the constant incubator of 30 DEG C, utilizes culture dish that spore is sowed at seedling medium that moisture ratio is 40%, 52%, 64%, 76%, 88%, 100% respectively: to sow in the formulation media of garden mould=1:3.
Result
When formulation media water content is 88%, spore began to show green in stromal surface at the 12nd day, occurred in a large number at 21 days gametocytes, and when the 50th day, the 1st strain sporophyte seedling occurred, within 90 days, had 20% Development of Gametophytes to become sporinite；Substrate moisture ratio spore germination situation between 64%～76% with moisture content 76% better, occurs in a large number at 22 days gametocytes, and when the 80th with the day after tomorrow, the 1st strain sporophyte seedling occurs, but within 90 days, only has 3% Development of Gametophytes and become sporinite.The visible formulation media moisture ratio being best suitable for Spora Lygodii growth is 88%.
Test example 3, the suitableeest Spora Lygodii spore germination based assays
Gathering the wild Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, when Sporangium splits after 5～7d, spore is scattered naturally, is collected by the spore on thin film, standby with sealing in the packed constant temperature refrigerator sealing and being saved in 5 DEG C up for safekeeping；
Matrix treatments is tested
JIUYUE is sowed, and selects the flowerpot nursery of high 25cm, diameter 30cm in booth；Choose high-temperature sterilization peat soil, high-temperature sterilization garden mould, peat soil of not sterilizing, garden mould of not sterilizing, the substrate 5 kinds different such as fresh lichen of not sterilizing as Germination textures.Each substrate is all spread this substrate of 2～3cm and is crossed the 80 thin substrate of purpose on top layer；The test group garden mould spreading fresh lichen fills basin, the top layer fresh lichen of kind last layer (need not sieve).Drenching water, then sow, thickness of sowing is 0.5g/.Cover the glass sterilized, keep humidity.
Result
See shown in table 1 below.
The situation of Spora Lygodii spore germination in table 1, different substrates
It can be seen from the results above that Spora Lygodii spore germination is had significant impact by physical property in soil different in natural temperature, glass room.Data are respectively organized surveying it after 90 days breed.From upper table, known Spora Lygodii spore is crossed Seeded growth in peat soil substrate in high-temperature sterilization and is obtained best, and in unit are, gametocyte and sporinite are maximum, up to 47.72 strains/cm2, wherein sporinite conversion ratio is up to 64.2%, and individual plant comprehensive plant strain growth situation (comparing with Weight per plant) is best.
Test example 4, the suitableeest Spora Lygodii spore germination shade rate are tested
Gathering the wild Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, when Sporangium splits after 5～7d, spore is scattered naturally, is collected by the spore on thin film, standby with sealing in the packed constant temperature refrigerator sealing and being saved in 5 DEG C up for safekeeping；
Shade rate is tested
JIUYUE is sowed, with the flowerpot nursery of high 25cm, diameter 30cm in booth.Flowerpot bottom loads garden mould, spreads and crosses 80 mesh sieve garden moulds into top 2～3cm, through the peat soil of high-temperature sterilization.Thiophanate (illustratively) overlay film one of drenching is late.Utilize the shade net of different densities, be respectively set to 90%, 75%, 50%, 4 kinds of shade rates of full exposure process.Drenching water, then sow, thickness of sowing is 0.5g/, repeats 3 times.Cover sterilization glass again, keep humidity；
Result
See shown in table 2 below.
Spora Lygodii spore germination information slip in table 2, different shade rate
By the above results it can be seen that under identical natural conditions, sprouted through 90 days, it is maximum that spore every basin when shade rate is 90% obtains sporinite number；When shade rate is 75%, individual plant comprehensive plant strain growth situation is best.Visible spore is little to intensity of illumination demand at the rudiment initial stage, but illumination still has when developing into the sporinite seedling of high-quality certain demand.
Test example 5, the suitableeest Spora Lygodii spore germination thickness of sowing are tested
Gathering the wild Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, when Sporangium splits after 5～7d, spore is scattered naturally, is collected by the spore on thin film, standby with sealing in the packed constant temperature refrigerator sealing and being saved in 5 DEG C up for safekeeping；
Different thickness of sowing tests
JIUYUE is sowed, and loads garden mould in flowerpot bottom, covers the glass sterilized, be placed in booth, and thiophanate overlay film one of drenching is late.Then top spreads the peat soil through high-temperature sterilization into mistake 80 mesh sieve thick for 2～3cm, water of drenching so that it is humidity is maintained at 64%～88%, thickness of sowing be seeded in above-mentioned peat soil, cover the glass sterilized, then cover the shade net that shade rate is 75%, within second day, press 0.0625g/m respectively2、0.125g/m2、0.250g/m2、0.375g/m2、0.500g/m2、1.0/m25 kinds of thickness of sowing sowings.
Result
See shown in table 3 below.
Table 3, different thickness of sowing Spora Lygodii spore germination situation
It can be seen from the results above that 1.0g/m2Go mouldy after 30 days serious, almost entirely destroy.Wherein, thickness of sowing is 0.5g/m2Maximum 3.22 strains of the sporinite/cm obtained2, but the worst Weight per plant 0.011g of the comprehensive growing state of plant.Secondly, thickness of sowing is 0.375g/m2The sporinite 1.45 strains/cm obtained2, but comprehensive plant strain growth situation is best, and Weight per plant is 0.036g.Visible, thickness of sowing is 0.5g/m2Time, Spora Lygodii spore germination is the maximum of sporinite, and spore thickness of sowing is 0.375g/m2Time the comprehensive growing state of individual plant plant best.0.25g/m2The comprehensive growing state of individual plant plant and 0.375g/m2Close, when therefore needing to obtain healthy and strong Spora Lygodii seedling, can by 0.25～0.375/m2Carry out sowing and breeding.
Test example 6, the suitableeest Spora Lygodii spore germination fertilizer test
Gathering the wild Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, when Sporangium splits after 5～7d, spore is scattered naturally, is collected by the spore on thin film, standby with sealing in the packed constant temperature refrigerator sealing and being saved in 5 DEG C up for safekeeping；
Different fertilizer is tested
JIUYUE is sowed, and loads garden mould in flowerpot bottom, covers the glass sterilized, be placed in booth, and thiophanate overlay film one of drenching is late.Then top spreads the peat soil through high-temperature sterilization into mistake 80 mesh sieve thick for 2～3cm, drench water, its humidity is made to be maintained at 64%～88%, thickness of sowing be seeded in above-mentioned peat soil, cover the glass sterilized, then cover the shade net that shade rate is 75%, sow by 0.5g/, fertilising within 30th day, is started in sowing, 10 days/1 time.Apply complete good good fortune 0.05% respectively, Hai Wei executes 0.05%, compound fertilizer 0.05%, ck4 kind fertilising (water fertilising) process.
Result
Result is shown in table 4 below.
Table 4, Different Fertilization Spora Lygodii spore germination situation
Spora Lygodii seedling is had different impacts (see table 4) by different fertilizer.Within 30th day, start fertilising from nursery, within every 10 days, apply fertilizer 1 time.Sporinite growing state is surveyed after 2 months.From each fertilising group growing state: that sporinite is maximum is matched group (ck) 10.72 strains/cm not applied fertilizer2, other obtains sporinite and how much is followed successively by and executes complete good good fortune 9.22 strains/cm2> Shi Haiweishi 8.22 strains/cm2> sealing fertilizer 6.28 strains/cm2.But getting well much from what the ratio of the comprehensive growing state fertilising of individual plant or not, other Weight per plant order is followed successively by the Shi Haiweishi > Quan Jiafu > compound fertilizer > matched group not applied fertilizer and executes.
Test example 7, the suitableeest Spora Lygodii spore Transplanting Test
Gathering the wild Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, when Sporangium splits after 5～7d, spore is scattered naturally, is collected by the spore on thin film, standby with sealing in the packed constant temperature refrigerator sealing and being saved in 5 ± 1 DEG C up for safekeeping；
Different size seedling replanting is tested
In the glass room flowerpot nursery with high 12cm, diameter 8cm, press gametocyte (original foliage) and sporinite (1 leaf, 3 leaves, plant height 1～2cm, plant height 2～4cm, plant height 4～8cm, plant height 8～14cm) 7 kinds process respectively, repeat 3 times.Within 90 days, surveying data in sowing, every group surveys 30 strains, and the survival rate that research Spora Lygodii seedling is transplanted under the much conditions of plant is the highest.
Sowing
Loading garden mould in flowerpot bottom, cover the glass sterilized, be placed in booth, thiophanate overlay film one of drenching is late.Then top spreads the peat soil through high-temperature sterilization into mistake 80 mesh sieve thick for 2～3cm, and water of drenching so that it is humidity is maintained at 64%～88%, then by the spore after incubator is cultivated by 0.25～0.375g/m2Thickness of sowing be seeded in above-mentioned peat soil, cover the glass sterilized, then cover the shade net that shade rate is 75%, in sowing within the 30th day, start its Shi Haiwei is applied fertilizer, 10 days/1 time.
Transplant
Grow up to the sporinite of 8～14cm after 90 days, be transplanted to mass ratio be 3:1 garden mould and peat soil composition substrate in.
Result
Result is shown in shown in table 5 below:
Table 5, Spora Lygodii different size seedling replanting situation
It can be seen from the results above that in glass room under natural conditions, its later stage growing state is had different impacts after different size of Spora Lygodii seedling replanting.Sporinite growing state (see table 5) is surveyed after transplanting 4 months.From each group of growing state: what transplanting survival rate was the highest is sporinite 8-14cm group, and its survival rate is up to 100%；This 3 group survival rate of 1-2cm, 3 leaf 2-4cm, 4-8cm is more or less the same, and all can reach more than 92%；The transplanting survival rate of 1 leaf group is also up to 66.67%；The transplanting survival rate worst 51.85% of gametocyte group.Also it is sporinite 8-14cm group > sporinite 4-8cm > sporinite 3 leaf 2-4cm > sporinite 1 leaf > 1 leaf > gametocyte from the comprehensive growing state of individual plant.Visible Spora Lygodii transplanting survival rate is directly proportional to the comprehensive growing state of individual plant, transplant select Seedling need to reach a certain size to its transplant after Spora Lygodii survive relatively larger with growth effect.
Claims (10)
1. a germination method for Spora Lygodii spore, described germination method comprises the steps:
1) collect seed
Gather the spore of Spora Lygodii plant, dress envelope to be saved in constant temperature refrigerator standby；
2) prepare before sowing
Fill garden mould, drench carbendazim or thiophanate liquid in flowerpot bottom, cover transparent membrane and seal 1～2 day, then spread 2～3cm thick, through the peat soil substrate of high-temperature sterilization, water of drenching；
3) sowing
By step 1) standby spore presses 0.25g～0.375g/m2Thickness of sowing be seeded in peat soil substrate top layer；
4) daily management
Cover glass, maintain temperature 20～30 DEG C, intensity of illumination is 2000～3000lx, light application time is 12h/d or to cover shade rate be the shade net of 75%, and keep soil moisture 60%～88%, air humidity be 80%～90% greenhouse in cultivate；
5) strong sprout
Within 30th day, proceed by fertilising in sowing, within every 10 days, apply fertilizer 1 time；
6) transplant for the first time
It is transplanted to nursery in equipped with the nutrient cup of seedling medium when juvenile sporophyte grows 2～3 accessory pinnas, plant 8～14cm height.
2. germination method according to claim 1, it is characterized in that, described step 1) be: the acquisition time of spore is 8～JIUYUE in summer, gathering the Spora Lygodii plant with maturation spore, be then placed on thin film and naturally dry in the shade, after 5～7d, Sporangium splits, spore is scattered naturally, being collected by spore, seal up for safekeeping with sealing is packed, dress seals and is saved in the constant temperature refrigerator of 5 ± 1 DEG C standby.
3. germination method according to claim 1, it is characterised in that step 2) in, at the garden mould of built-in 4/5 volume of flowerpot, its top layer is twisted with the fingers thin with hands；Described peat soil substrate was 80 mesh sieves the peat soil substrate through 120 DEG C of high-temperature sterilizations.
4. germination method according to claim 1, it is characterised in that step 2) in, described water of drenching is that the mode adopting spray painting is drenched water.
5. germination method according to claim 1, it is characterised in that step 3) in, described sowing is seeded in peat soil substrate top layer equably for being stained with spore powder with clean brush pen.
6. germination method according to claim 1, it is characterised in that step 5) in, described fertilising adopts spray painting, the thin mode executed to apply fertilizer.
7. germination method according to claim 1, it is characterised in that step 6) in, the mixed-matrix that described seedling medium is garden mould and peat soil forms with the mass ratio of 3:1.
8. germination method according to claim 7, it is characterised in that carrying out step 6) described in transplanting time drench and determine root water.
9. the germination method according to claim 1～8 any one, it is characterised in that after planting, after Spora Lygodii gametocyte is formed, opens the glass ventilation 1～2h covering on flowerpot every day and sprays 1 time.
10. germination method according to claim 9, it is characterised in that step 2) in, the sowing time of described sowing is mid-September in autumn, selects the flowerpot nursery of high 25cm, diameter 30cm in booth.
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| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN107593290A (en) * | 2017-09-01 | 2018-01-19 | 桂林三金药业股份有限公司 | A kind of implantation methods of Lygodium japonicum |
| CN113287483A (en) * | 2021-05-25 | 2021-08-24 | 重庆市药物种植研究所 | Rhizoma drynariae spore direct seeding germination substrate and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102742447A (en) * | 2012-07-27 | 2012-10-24 | 丰城市永生农业发展实业有限公司 | Artificial cultivation method for Japanese climbing fern spore |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102742447A (en) * | 2012-07-27 | 2012-10-24 | 丰城市永生农业发展实业有限公司 | Artificial cultivation method for Japanese climbing fern spore |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN107593290A (en) * | 2017-09-01 | 2018-01-19 | 桂林三金药业股份有限公司 | A kind of implantation methods of Lygodium japonicum |
| CN113287483A (en) * | 2021-05-25 | 2021-08-24 | 重庆市药物种植研究所 | Rhizoma drynariae spore direct seeding germination substrate and use method thereof |
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