CN105779527A - Method for increasing alkaline pretreated wood fiber raw material enzymolysis yield - Google Patents
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Abstract
The invention discloses a method for increasing the alkaline pretreated wood fiber raw material enzymolysis yield. The method comprises the steps of raw material pretreatment and enzyme hydrolysis, wherein in the step of pretreatment, polyethylene glycol diglycidyl ether is added to co-treat a substrate. The method for increasing the alkaline pretreated wood fiber raw material enzymolysis yield has the beneficial effects that during low-temperature alkaline pretreatment, polyethylene glycol diglycidyl ether is added to co-treat the substrate, thus effectively weakening nonspecific adsorption of lignin toward cellulase in the enzyme hydrolysis process and being beneficial to promoting cellulase hydrolysis; test results show that compared with existing research results, the enzyme hydrolysis yield is increased by 39.75% and reaches 71.26%; meanwhile, the method is simple in pretreatment process conditions, mild in reaction conditions and high in carbohydrate recovery rate, has the advantages of low energy consumption, medicine recovery, and the like, further has actual operation value and has good practicability.
Description
Technical field
The present invention relates to the preparation technology of fermentable sugar, be specifically related to a kind of method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield.
Background technology
Along with human society to the growing of fossil resource demand and exhaustive exploitation, on the earth, fossil resource is inevitably faced with exhausted destiny totally；Greenhouse effect that the use of a large amount of fossil resources causes and the haze broken out in recent years pollute and then develop into as the encountered another a major challenge of the mankind；Meanwhile, " white pollution " caused that use in a large number of the non-degradable plastic being raw material production with petroleum resources has become society's one big public hazards, urgent need searching degradable new material.Therefore, China's Energy restructuring is very urgent, utilizes reproducible biomass resource to produce bioenergy, bio-based chemicals and biomaterial are the permanent mechanism of China's sustainable development.
Semen Maydis is the cereal crops that China is important, and annual produced corn straw total amount is extremely considerable.The atmosphere polluting problem that the burning of corn straw causes is urgently to be resolved hurrily, and therefore the scale of corn straw utilizes and also receives much concern.Lignocellulose raw material biorefinery becomes the energy or chemicals to be one of corn straw scale, effective approach utilized.Cellulose in corn straw can be biodegradable into monosaccharide under cellulase catalytic, is then fermented into the various energy or chemicals by different microorganisms.The same with other cellulose castoff, in corn straw, cellulose, hemicellulose and lignin form the structure of densification, are unfavorable for that cellulase is to cellulosic effect.Wherein, in substrate, cellulase hydrolysis is had inhibition by the existence of lignin, and its suppression mechanism includes the sterically hindered effect to cellulase attack and the ineffective adsorption effect to cellulase.Generally, it is considered that delignification rate is more high in preprocessing process, in lignocellulose raw material, cellulose exposes more many, is more conducive to follow-up cellulase hydrolysis.Alkaline process pretreatment and organic solvent pretreatment are notable to the removal effect of lignin, but owing to pre-processing device is required height by organic solvent pre-treatment, and have the problem such as potential safety hazard of blast, and industrial applications probability is relatively low.Alkaline process pretreatment is usually used in pulping and paper-making industry, and the medicine recovery technology of maturation makes one of alkaline process pretreatment common method becoming wood fibre pretreatment.In alkaline process preprocessing process, the ehter bond of lignin and ester bond rupture in a large number so that a large amount of dissolution of lignin.Research shows, the removal efficiency of alkali charge and pretreatment temperature and lignin is proportional.But in oxygenation pretreatment process, too high alkali charge and pretreatment temperature can cause serious peeling reaction, thus reducing the response rate of cellulose and hemicellulose.
The suppression degree of cellulase hydrolysis is not limited only by the impact of content of lignin by lignin, closely related with the physicochemical property of lignin and chemical constitution yet.Residual lignin in oxygenation pretreatment material can pass through adsorptive cellulose enzyme, reduces effective fiber element enzyme concentration, thus increasing the cost of cellulase consumption and enzyme hydrolysis.Interaction force between lignin and cellulase protein mainly includes hydrophobic interaction, electrostatic interaction and hydrogen bond action.Lot of documents reports can intervene the interaction between cellulase and lignin by interpolation nonionic surfactant, thus improving performing enzyme hydrolysis on wood fiber raw material performance.Report lignin in recent years and prepare amphipathic lignin-base surfactant through polyethoxy grafting and modifying.Cellulase hydrolysis process is added lignin-base surfactant, cellulase hydrolysis can be remarkably promoted equally.At present, polyethoxy grafting and modifying method only for lignin sample, need to will carry out chemical modification, then be added in enzyme hydrolysis system after lignin separation in raw material, thus playing the effect improving pretreated material enzyme hydrolysis yield.This technical process is excessively complicated, is not suitable for large-scale industrial and produces, and therefore needs to simplify polyethoxy grafting and modifying lignin and improves the technical process of cellulase hydrolysis yield.
Summary of the invention
Goal of the invention: for the deficiencies in the prior art, it is an object of the invention to provide the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, by adding polyethoxy ether coprocessing substrate in preprocessing process, reach to improve the purpose of enzyme hydrolysis yield.
Technical scheme: in order to realize foregoing invention purpose, the technical solution used in the present invention is as follows:
A kind of method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, including pretreatment of raw material and enzyme hydrolysis step；In described pre-treatment step, add polyethyleneglycol diglycidylether coprocessing substrate.
Described polyethyleneglycol diglycidylether g/g consumption is 5-30%, by material over dry restatement, it is preferred to 10%.
Described pre-treatment step is: lignocellulose raw material is in alkali consumption 10-40%, polyethyleneglycol diglycidylether consumption 10%, solid-to-liquid ratio 1:10g/mL, coprocessing 3h at 70 DEG C.It is preferably: lignocellulose raw material is in alkali consumption 10%, polyethyleneglycol diglycidylether consumption 10%, solid-to-liquid ratio 1:10g/mL, coprocessing 3h at 70 DEG C.
In described enzyme hydrolysis step, enzyme used is cellulase, is the complex of one or more enzymes of the cellulase becoming glucose with Trichoderma spp., aspergillosis or bacteriogenic energy degraded cellulose.
Described enzyme hydrolysis cellulose g/mL concentration is 2-10%.
The described method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, specifically comprises the following steps that
1) lignocellulose raw material pretreatment solid-to-liquid ratio is 1:10, and alkali consumption is 10-40%, and polyethyleneglycol diglycidylether consumption is 5-30%, and pretreatment time is 1.5-4.0h；
2), after pretreatment terminates, isolated by filtration obtains pretreated material, and through washing, filter, wash away the alkali liquor of remnants, the lignin of dissolution and sugar；
3) above-mentioned pretreatment corn straw is mixed with cellulase, add water, pH buffer, acid or alkali, mix to cellulose concentration 2-10%, control ph is at 4.0-6.0, in reaction system, every gram of cellulosic cellulase consumption is 10-40FPU, and at 45-55 DEG C, enzyme digestion reaction 48-72h obtains fermentable sugar.
The described method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, it is preferable that specifically comprise the following steps that
1) lignocellulose raw material pretreatment solid-to-liquid ratio is 1:10, and alkali consumption is 10%, and polyethyleneglycol diglycidylether consumption is 10%, and pretreatment time is 3h；
2), after pretreatment terminates, isolated by filtration obtains pretreated material, and through washing, filter, wash away the alkali liquor of remnants, the lignin of dissolution and sugar；
3) above-mentioned pretreatment corn straw is mixed with cellulase, add water, pH buffer, acid or alkali, mix to cellulose concentration 2-10%, control ph is at 4.0-6.0, in reaction system, every gram of cellulosic cellulase consumption is 10-40FPU, and at 45-55 DEG C, enzyme digestion reaction 48-72h obtains fermentable sugar.
Beneficial effect: compared with prior art, the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield of the present invention, low-temperature alkaline pretreatment is added polyethyleneglycol diglycidylether coprocessing substrate, effectively weaken the lignin non-specific adsorption to cellulase in enzyme hydrolysis process, may advantageously facilitate the carrying out of cellulase hydrolysis.Result of the test shows, compared with existing result of study, the enzyme hydrolysis yield of this patent improves 39.75%, reaches 71.26%.This method pretreating process condition is simple simultaneously, and reaction condition is gentle, and the carbohydrate response rate is high, and has the advantage such as low energy consumption and medicine recovery, has more practical operation and is worth, has good practicality.
Accompanying drawing explanation
Fig. 1 is free protein content result figure in pretreatment Factor of Enzymolysis Corn Stalk process.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further elaborated.Embodiment is for the illustrative not limiting present invention.In this area, any those of ordinary skill is it will be appreciated that these embodiments, does not limit the present invention in any way, can make suitable amendment and without prejudice to the essence of the present invention and deviation the scope of the present invention.
In following example, glucose, xylose and cellobiose concentration adopt high performance liquid chromatography (HPLC) to measure.Chromatographic condition is as follows: chromatograph: Agillent1200 high performance liquid chromatograph；Chromatographic column: Bio-RadAminexHPX-87H；Mobile phase: 0.005mol/L sulphuric acid, flow velocity: 0.6mL/min；Column temperature: 55 DEG C；Detector: differential refraction detector；Sample size: 10 μ L.External standard method.
In embodiment, pretreatment alkali consumption is with corn straw over dry restatement, for alkali consumption 10% (g/g), refers to pretreatment 100g over dry raw material, and alkali consumption is 10g；Polyethyleneglycol diglycidylether consumption is with corn straw over dry restatement, for polyethyleneglycol diglycidylether consumption 10% (g/g), refers to pretreatment 100g over dry raw material, and polyethyleneglycol diglycidylether consumption is 10g.
Embodiment 1
A kind of method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, step is as follows:
1) over dry corn straw 100g is weighed respectively, respectively with 10%, 20%, 30%, 40% (g/g, lower same) sodium hydroxide, in solid-to-liquid ratio 1:10 (g/mL, lower same), polyethyleneglycol diglycidylether consumption is 10% (g/g, lower same), pretreatment 3h at 70 DEG C.
2), after pretreatment terminates, material is through washing and collects by filtration.The Principle components analysis of polyethoxy ether pretreatment corn straw is in Table 1.
(3) weigh the corn straw that the above-mentioned alkali consumption that cellulose over dry is heavily 1.00g is 10%, 20%, 30%, 40% and polyethyleneglycol diglycidylether consumption is 10% pretreatment respectively and be hydrolyzed in bottle in 2 250mL, add 1mol/L citrate buffer solution 2.5mL in each hydrolysis bottle, enzyme dosage is the cellulosic cellulase of 20FPU/g and appropriate distilled water, making moisture cumulative volume in enzymatic hydrolysis system is 50mL, cover lid after reaction system fully being mixed with glass rod, enzymolysis 72h in 150rpm, the constant-temperature table of 50 DEG C.
After enzymolysis terminates, respectively by hydrolysate centrifuge centrifugal 5min in the 10000rpm when, taking supernatant and measure concentration of glucose therein and cellobiose concentration, and calculate enzyme hydrolysis yield, data take double; two parallel test meansigma methods.Wherein, cellulase hydrolysis yield (%)=(in hydrolyzed solution cellobiose concentration g/L × 0.95 in concentration of glucose g/L × 0.9+ hydrolyzed solution) × 0.05 ÷ (substrate over dry weight g × content of cellulose) × 100%.In formula: 0.9 is glucose and cellulosic conversion coefficient；0.95 is cellobiose and cellulosic conversion coefficient；0.05 is hydrolyzed solution volume, L.
The Principle components analysis of polyethoxy ether pretreatment corn straw 72h and enzymolysis yield are in Table 1.
The main component of table 1 polyethoxy ether pretreatment corn straw and enzymolysis yield
Show from table 1 result, under identical polyethyleneglycol diglycidylether consumption (10%, g/g), increase along with pretreatment alkali consumption, in pretreatment corn straw, beta-dextran content increases, and xylan and sour insoluble content of lignin significantly reduce, cellulase hydrolysis yield significantly improves simultaneously.
Embodiment 2
A kind of method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, step is as follows:
1) over dry corn straw 100g is weighed respectively, with 10% (g/g) sodium hydroxide, in solid-to-liquid ratio 1:10 (g/mL), polyethyleneglycol diglycidylether consumption is 5%, 10%, 20%, 30% (g/g) respectively, pretreatment 3h at 70 DEG C.
2), after pretreatment terminates, material is through washing and collects by filtration.The Principle components analysis of polyethoxy ether pretreatment corn straw is in Table 2.
(3) weighing cellulose over dry respectively is heavily 10% for the above-mentioned alkali consumption of 1.00g, the corn straw that polyethyleneglycol diglycidylether consumption is 5%, 10%, 20%, 30% pretreatment is hydrolyzed in bottle in 2 250mL, add 1mol/L citrate buffer solution 2.5mL in each hydrolysis bottle, enzyme dosage is the cellulosic cellulase of 20FPU/g and appropriate distilled water, making moisture cumulative volume in enzymatic hydrolysis system is 50mL, cover lid after reaction system fully being mixed with glass rod, enzymolysis 72h in 150rpm, the constant-temperature table of 50 DEG C.
After enzymolysis terminates, respectively by hydrolysate centrifuge centrifugal 5min in the 10000rpm when, taking supernatant and measure concentration of glucose therein and cellobiose concentration, and calculate enzyme hydrolysis yield, data take double; two parallel test meansigma methods.The Principle components analysis of polyethoxy ether pretreatment corn straw 72h and enzymolysis yield are in Table 2.
The main component of table 2 polyethoxy ether pretreatment corn straw and enzymolysis yield
From table 2 it is shown that under identical alkali consumption (10%, g/g), along with the increase of polyethyleneglycol diglycidylether consumption, in pretreatment corn straw, sour insoluble content of lignin is gradually increased, and pretreated material enzymolysis yield gradually rises.When polyethyleneglycol diglycidylether consumption is 10%, enzymolysis yield is increased to 69.07%.Increasing polyethyleneglycol diglycidylether consumption further, enzymolysis yield does not present significant raising.
Embodiment 3
A kind of method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, cellulase absorption situation test, specifically comprise the following steps that
1) 100g over dry corn straw is weighed, in alkali consumption 10% (g/g), solid-to-liquid ratio is 1:10 (g/mL), and pretreatment 3h at polyethyleneglycol diglycidylether consumption 10% (g/g) 70 DEG C collects by filtration pretreatment fluid and pretreated material.
2) pretreated material through washing and collects by filtration, and operates for cellulase hydrolysis.
3) weighing cellulose over dry respectively is heavily 10% for the above-mentioned alkali consumption of 1.00g, the corn straw that polyethyleneglycol diglycidylether consumption is 10% pretreatment is hydrolyzed in bottle in 2 250mL, add 1mol/L citrate buffer solution 2.5mL in each hydrolysis bottle, enzyme dosage is the cellulosic cellulase of 20FPU/g and appropriate distilled water, making moisture cumulative volume in enzymatic hydrolysis system is 50mL, cover lid after reaction system fully being mixed with glass rod, enzymolysis 72h in 150rpm, the constant-temperature table of 50 DEG C.
4) in enzymolysis process, sample respectively at 3h, 6h, 12h, 24h, 48h, 72h, with centrifuge centrifugal 5min in the 10000rpm when, take supernatant and measure the concentration of wherein resolvase albumen, and calculating relative protein content, data take double; two parallel test meansigma methods.Wherein, resolvase protein concentration (%)=(in supernatant pheron concentration g/L) ÷ (the total pheron concentration g/L added in enzymatic hydrolysis system) × 100%.
To be not added with polyethyleneglycol diglycidylether for compareing with process, carry out simultaneous test.Result is as shown in Figure 1, it was shown that in the enzymolysis process of test sample, in enzymolysis supernatant free pheron concentration present fall before after the trend that rises.During enzymolysis 3h, resolvase protein content is the 57.1% of total protein content.After enzymolysis carries out 24h, resolvase protein content drops to 34.7%.After enzymolysis 72h, in enzymolysis supernatant, free pheron concentration is 66.3%.In the enzymolysis process of control sample, in enzymolysis supernatant free pheron concentration present equally fall before after the trend that rises.During enzymolysis 3h, resolvase protein content is the 39.8% of total protein content, far below the resolvase protein content (57.1%) in test sample 3h enzymolysis supernatant.After enzymolysis 72h, in the supernatant of control sample, free pheron concentration is 29.59%, is also preferably below the resolvase protein content (66.3%) in test sample 72h enzymolysis supernatant.Illustrate that adding polyethyleneglycol diglycidylether pretreatment altogether reduces the pretreated material non-specific adsorption to cellulase.
Comparative example 1
A kind of method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, with embodiment 1, but when alkalescence is with process without polyethyleneglycol diglycidylether, specifically comprises the following steps that
1) over dry corn straw 100g is weighed respectively, respectively with 10%, 20%, 30%, 40% (g/g) sodium hydroxide, in solid-to-liquid ratio 1:10 (g/mL), pretreatment 3h at 70 DEG C.
2), after pretreatment terminates, material is through washing and collects by filtration.The Principle components analysis of pretreatment corn straw is in Table 3.
3) weigh cellulose over dry respectively and be heavily hydrolyzed in bottle for the corn straw that above-mentioned alkali consumption is 10%, 20%, 30%, 40% (g/g) pretreatment of 1.00g in 2 250mL, add 1mol/L citrate buffer solution 2.5mL in each hydrolysis bottle, enzyme dosage is the cellulosic cellulase of 20FPU/g and appropriate distilled water, making moisture cumulative volume in enzymatic hydrolysis system is 50mL, cover lid after reaction system fully being mixed with glass rod, enzymolysis 72h in 150rpm, the constant-temperature table of 50 DEG C.
After enzymolysis terminates, respectively by hydrolysate centrifuge centrifugal 5min in the 10000rpm when, taking supernatant and measure concentration of glucose therein and cellobiose concentration, and calculate enzyme hydrolysis yield, data take double; two parallel test meansigma methods, and result is as shown in table 3.
Table 3 is not added with main component and the enzymolysis yield of polyethoxy ether pretreatment corn straw
Table 3 is it is shown that along with the increase of pretreatment alkali consumption, in oxygenation pretreatment corn straw, beta-dextran content is consequently increased, and xylan and sour insoluble content of lignin decrease.Compared with the result of embodiment 1, under identical alkali consumption (10%), in embodiment 1, sour insoluble content of lignin is 12.59% (table 1), the sour insoluble content of lignin (9.98%) higher than in the present embodiment.Additionally, under identical pretreatment alkali consumption, the enzymolysis yield of the present embodiment is below the enzymolysis yield of embodiment 1, it it is 10% time at pretreatment alkali consumption, the enzymolysis yield of embodiment 1 is 71.26% (table 1), improves 39.75% than the enzymolysis yield (50.99%) of the present embodiment.
Comparative example 2
A kind of method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, for adding the conventional alkaline pretreatment Factor of Enzymolysis Corn Stalk method of lignin-base surfactant, specifically comprises the following steps that
1) weighing 100g over dry corn straw, in alkali consumption 10% (g/g), solid-to-liquid ratio is 1:10 (g/mL), and pretreatment 3h at 70 DEG C collects by filtration pretreatment fluid and pretreated material.
2) pretreated material through washing and collects by filtration, and operates for cellulase hydrolysis.
3) add concentrated sulphuric acid, pretreatment fluid pH is adjusted to less than 4.0, precipitate alkali lignin, and wash, filter, for the preparation of lignin-base surfactant.
4) weigh 20g over dry alkali lignin, in alkali consumption 40% (g/g), polyethyleneglycol diglycidylether consumption 200% (g/g), solid-to-liquid ratio 1:10 (g/mL), at 70 DEG C, react 2h.PH is adjusted to less than 4.0 after terminating by reaction, precipitates unmodified lignin.Take the lignin-base surfactant (final concentration 50g/L) after supernatant is modified by ultra-filtration and separation, concentration after centrifugal, add experiment for subsequent enzymatic hydrolysis.
5) weigh cellulose over dry and be heavily hydrolyzed in bottle for the corn straw that alkali consumption is 10% (g/g) pretreatment of 1.00g in 2 250mL, add above-mentioned lignin-base surfactant 0.5mL, 1mol/L citrate buffer solution 2.5mL in each hydrolysis bottle, enzyme dosage is the cellulosic cellulase of 20FPU/g and appropriate distilled water, making moisture cumulative volume in enzymatic hydrolysis system is 50mL, cover lid after reaction system fully being mixed with glass rod, enzymolysis 72h in 150rpm, the constant-temperature table of 50 DEG C.
After enzymolysis terminates, respectively by hydrolysate centrifuge centrifugal 5min in the 10000rpm when, taking supernatant and measure concentration of glucose therein and cellobiose concentration, and calculate enzyme hydrolysis yield, data take double; two parallel test meansigma methods.
Result shows, it is 69.08% that this comparative example adds the conventional alkaline pretreatment corn straw 72h enzymolysis yield of lignin-base surfactant, conventional alkaline pretreated material enzyme hydrolysis yield (50.99%) being not added with lignin-base surfactant than comparative example 1 improves 35.48%, but the enzyme hydrolysis yield (71.26%) of the method (embodiment 1) lower than this patent.
Visible, this method can remarkably promote enzyme hydrolysis, improves enzyme hydrolysis yield, and pretreating process condition is simple, has more practical operation and is worth.
Claims (9)
1. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield, including pretreatment of raw material and enzyme hydrolysis step；It is characterized in that: in described pre-treatment step, add polyethyleneglycol diglycidylether coprocessing substrate.
2. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to claim 1, it is characterised in that: described polyethyleneglycol diglycidylether g/g consumption is 5-30%, by material over dry restatement.
3. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to claim 1 and 2, it is characterised in that: described polyethyleneglycol diglycidylether g/g consumption is 10%, by material over dry restatement.
4. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to claim 1, it is characterized in that: described pre-treatment step is: lignocellulose raw material is in alkali consumption 10-40%, polyethyleneglycol diglycidylether consumption 10%, solid-to-liquid ratio 1:10g/mL, coprocessing 3h at 70 DEG C.
5. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to claim 1 or 4, it is characterized in that: described pre-treatment step is: lignocellulose raw material is in alkali consumption 10%, polyethyleneglycol diglycidylether consumption 10%, solid-to-liquid ratio 1:10g/mL, coprocessing 3h at 70 DEG C.
6. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to claim 1, it is characterized in that: in described enzyme hydrolysis step, enzyme used is cellulase, is the complex of one or more enzymes of the cellulase becoming glucose with Trichoderma spp., aspergillosis or bacteriogenic energy degraded cellulose.
7. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to claim 1, it is characterised in that: described enzyme hydrolysis cellulose g/mL concentration is 2-10%.
8. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to any one of claim 1,2,4,6,7, it is characterised in that specifically comprise the following steps that
1) lignocellulose raw material pretreatment solid-to-liquid ratio is 1:10, and alkali consumption is 10-40%, and polyethyleneglycol diglycidylether consumption is 5-30%, and pretreatment time is 1.5-4.0h；
2), after pretreatment terminates, isolated by filtration obtains pretreated material, and through washing, filter, wash away the alkali liquor of remnants, the lignin of dissolution and sugar；
3) above-mentioned pretreatment corn straw is mixed with cellulase, add water, pH buffer, acid or alkali, mix to cellulose concentration 2-10%, control ph is at 4.0-6.0, in reaction system, every gram of cellulosic cellulase consumption is 10-40FPU, and at 45-55 DEG C, enzyme digestion reaction 48-72h obtains fermentable sugar.
9. the method improving alkalescence pretreatment lignocellulose raw material enzymolysis yield according to claim 8, it is characterised in that specifically comprise the following steps that
1) lignocellulose raw material pretreatment solid-to-liquid ratio is 1:10, and alkali consumption is 10%, and polyethyleneglycol diglycidylether consumption is 10%, and pretreatment time is 3h；
2), after pretreatment terminates, isolated by filtration obtains pretreated material, and through washing, filter, wash away the alkali liquor of remnants, the lignin of dissolution and sugar；
3) above-mentioned pretreatment corn straw is mixed with cellulase, add water, pH buffer, acid or alkali, mix to cellulose concentration 2-10%, control ph is at 4.0-6.0, in reaction system, every gram of cellulosic cellulase consumption is 10-40FPU, and at 45-55 DEG C, enzyme digestion reaction 48-72h obtains fermentable sugar.
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| CN108893507A (en) * | 2018-07-23 | 2018-11-27 | 南京林业大学 | A method of it is pre-processed by combination and promotes sawdust enzymatic hydrolysis |
| CN111909976A (en) * | 2020-08-03 | 2020-11-10 | 南京林业大学 | Method for reducing process cost of preparing fermentable sugar by enzymatic hydrolysis of wood fiber raw material by using mannitol |
| CN114941254A (en) * | 2022-06-16 | 2022-08-26 | 淮阴工学院 | Pretreatment method and application of lignocellulose biomass and method for improving enzymolysis efficiency of lignocellulose biomass |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108893507A (en) * | 2018-07-23 | 2018-11-27 | 南京林业大学 | A method of it is pre-processed by combination and promotes sawdust enzymatic hydrolysis |
| CN111909976A (en) * | 2020-08-03 | 2020-11-10 | 南京林业大学 | Method for reducing process cost of preparing fermentable sugar by enzymatic hydrolysis of wood fiber raw material by using mannitol |
| CN111909976B (en) * | 2020-08-03 | 2022-07-12 | 南京林业大学 | Method for reducing process cost of preparing fermentable sugar by enzymatic hydrolysis of wood fiber raw material by using mannitol |
| CN114941254A (en) * | 2022-06-16 | 2022-08-26 | 淮阴工学院 | Pretreatment method and application of lignocellulose biomass and method for improving enzymolysis efficiency of lignocellulose biomass |
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