CN105777247A - Preparation method of selenium-enriched earthworm organic fertilizer - Google Patents
Preparation method of selenium-enriched earthworm organic fertilizer Download PDFInfo
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- CN105777247A CN105777247A CN201610059486.5A CN201610059486A CN105777247A CN 105777247 A CN105777247 A CN 105777247A CN 201610059486 A CN201610059486 A CN 201610059486A CN 105777247 A CN105777247 A CN 105777247A
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- lumbricus
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- 239000011669 selenium Substances 0.000 title claims abstract description 121
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 110
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 109
- 241000361919 Metaphire sieboldi Species 0.000 title claims abstract description 62
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000003895 organic fertilizer Substances 0.000 title abstract description 6
- 239000000758 substrate Substances 0.000 claims abstract description 48
- 239000003337 fertilizer Substances 0.000 claims abstract description 39
- 150000001413 amino acids Chemical class 0.000 claims abstract description 23
- 235000016709 nutrition Nutrition 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 claims abstract description 15
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 5
- 239000010871 livestock manure Substances 0.000 claims abstract description 4
- 229940091258 selenium supplement Drugs 0.000 claims description 102
- 241000243684 Lumbricus Species 0.000 claims description 44
- 238000009395 breeding Methods 0.000 claims description 35
- 230000001488 breeding effect Effects 0.000 claims description 35
- 239000000428 dust Substances 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 230000035764 nutrition Effects 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 18
- 239000011550 stock solution Substances 0.000 claims description 16
- 239000011781 sodium selenite Substances 0.000 claims description 15
- 239000007864 aqueous solution Substances 0.000 claims description 14
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 14
- 229960001471 sodium selenite Drugs 0.000 claims description 14
- 235000015921 sodium selenite Nutrition 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- 210000003608 fece Anatomy 0.000 claims description 12
- 244000144972 livestock Species 0.000 claims description 9
- 244000144977 poultry Species 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 241000243686 Eisenia fetida Species 0.000 claims description 2
- 238000003860 storage Methods 0.000 abstract description 3
- 241001233061 earthworms Species 0.000 abstract 3
- 238000000605 extraction Methods 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 238000002156 mixing Methods 0.000 abstract 2
- 238000000354 decomposition reaction Methods 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 238000005303 weighing Methods 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000002689 soil Substances 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000002366 mineral element Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910003424 Na2SeO3 Inorganic materials 0.000 description 1
- 206010039921 Selenium deficiency Diseases 0.000 description 1
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 1
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 231100000693 bioaccumulation Toxicity 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003342 selenium Chemical class 0.000 description 1
- 229960002718 selenomethionine Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0332—Earthworms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a preparation method of a selenium-enriched earthworm organic fertilizer. The method includes the following steps: in accordance with the mass ratio of 1:1, weighing fermented livestock manure and mushroom dregs, sieving, and mixing evenly to obtain a substrate for feeding earthworms; preparing a selenium-enriched feeding substrate and a normal feeding substrate; introducing the earthworms into the obtained selenium-enriched feeding substrate and the obtained normal feeding substrate, feeding, and separating the earthworms and wormcast; preparing an earthworm homogenate; carrying out enzymatic hydrolysis of the earthworm homogenate, to obtain a selenium-enriched amino acid nutritional original solution; preparing a wormcast extraction liquid; and mixing the wormcast extraction liquid and the selenium-enriched amino acid nutritional original solution, and sterilizing to obtain the fertilizer. The preparation method of the selenium-enriched earthworm organic fertilizer has the characteristics of low production cost and short production cycle; and the obtained product has the advantages of long storage time, complete earthworm decomposition, high organic selenium content and the like.
Description
Technical field
The present invention relates to agricultural organic fertilizer technical field, be more particularly to the manufacture method of a kind of selenium-enriched earthworm fertilizer.
Background technology
Selenium is that humans and animals bulk-growth grows required trace element, is also the beneficial element of plant growing.Selenium participates in multiple important functional enzyme in composition body, and maintenance health and normal physiological metabolism are had particularly important effect.China's Selenium in Soil maldistribution of the resources is weighed and there is large-area scarce selenium area, and this selemium nutrition directly resulted in the selenium source food that human body is main is under-supply, and then affects health.Additionally, since 20th century, inorganic fertilizer and pesticide a large amount of use cause create such as Soil structure destruction, soil property worsen, a series of great environmental problem and the agricultural problem such as the quality of agricultural product and safety reduction, the harm of human body is constantly increased, how to solve agricultural product selenium deficiency simultaneously and use the negative agricultural problem that inorganic fertilizer etc. brings to become vast researcher and planted a great problem that raiser faces.
Lumbricus contains the nutrient substance such as a large amount of protein, the aminoacid of abundant species, vitamin and mineral element, is that a kind of well amino acid foliage fertilizer makes material.Lumbricus Se accumulation amount is big, and by biotransformation, inorganic selenium can be converted into organic selenium, and transformation efficiency is high.Wormcast contains a certain amount of nitrogen, phosphorus, potassium, multiple mineral element and substantial amounts of profitable strain, is the organic fertilizer of a kind of high-quality.The fertilizer made currently with Lumbricus and wormcast is how fertile as solid-based, need to use with other fertilizer and Se content is relatively low, still can not meet the demand of plant selenium-rich, and some currently used fertilizer rich in selenium mostly are inorganic selenium fertilizer, chronic administration or use the security risk that improper existence is certain.
Summary of the invention
(1) to solve the technical problem that
The technical problem to be solved in the present invention is how to improve the Se content in fertilizer, and provides the manufacture method of selenium-enriched earthworm fertilizer.
(2) technical scheme
In order to solve above-mentioned technical problem, the invention provides the manufacture method of a kind of selenium-enriched earthworm fertilizer, the method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 3-5mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus;
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins the sodium selenite aqueous solution of 0.5-1.0L in the substrate of the 12.5-25kg weight that step one obtains;In the another substrate that a certain amount of water is joined the other 12.5-25kg weight that step one obtains;Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust;
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raise at 22-28 DEG C of temperature, once identical with former substrate substrate is added respectively every 15-20 days, after earthworm breeding 40-50 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm, standby;
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 20-26 hour under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate, obtains Lumbricus homogenate;
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 50-60 DEG C of condition, every stirring in 5-6 hour once, enzymolysis 20-26 hour, obtain selenium-rich amino acid nutrition stock solution;The present invention need not additional fermenting agent, cost is low, and efficiency is high, simple to operate.
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5-6, it is sufficiently stirred at ambient temperature, it is filtered after stirring 3-5 hour, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed by a certain percentage, by mixed liquor sterilizing 1.5-2.5 hour under 100 DEG C of conditions, then cooling, filtration, to obtain final product.Sterilizing is to prevent in later stage storage process owing to bacterial activity produces rotten, fouling phenomenon, extending fertilizer period of storage.
Preferably, described feces of livestock and poultry is cattle manure.
Preferably, described dreg is the residue of mushroom culture medium material, it is preferable that the residue of culture medium for golden mushroom material.
Preferably, described Lumbricus is girdled adult Eisenia foetida.Because the compatriot that grows up likes that victory earthworm bioaccumulation and the ability being converted into organic selenium are strong.
Preferably, in step 6, described wormcast extracting solution mixes according to volume ratio 1:0.8-1.2 with selenium-rich amino acid nutrition stock solution.
Preferably, described method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 4mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus;
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins 0.8L sodium selenite aqueous solution in the substrate of the 20kg weight that step one obtains;In the another substrate that a certain amount of water is joined the other 20kg weight that step one obtains;Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust;
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 18 days respectively, earthworm breeding is after 45 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm;
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 24 hours under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate;
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 55 DEG C of conditions, every stirring in 5.5 hours once, enzymolysis 24 hours, obtain selenium-rich amino acid nutrition stock solution;
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5.5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 4 hours, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed according to volume ratio 1:1, by mixed liquor sterilizing 2 hours under 100 DEG C of conditions, then cooling, filtration, to obtain final product.
Present invention also offers the selenium-enriched earthworm fertilizer that the manufacture method of described selenium-enriched earthworm fertilizer prepares, its total Se content is about 8-12mg/L, and wherein organic selenium content accounts for more than 96%.
Preferably, the pH value of described selenium-enriched earthworm fertilizer is 6.8, close to neutral.This is advantageously in plant stem-leaf absorbing fertilizer Middle nutrition element.
Selenium-enriched earthworm fertilizer of the present invention according to the nutritional need of Different Crop and fruit and vegerable, directly can use after diluting suitable multiple.
(3) beneficial effect
The present invention adopts fermentation cattle manure and dreg to be substrate, and artificially adds selenium (Na2SeO3) raise ripe compatriot and love victory Lumbricus, enrichment and to convert the efficiency of organic selenium high, and selenium-enriched earthworm is to make the material that selenium-rich amino acid is fertile fabulous.Furthermore, containing the trace element such as a large amount of nitrogen, phosphorus, potassium element and copper, ferrum, zinc in common wormcast, and amino acid classes and rich content, it is the SOLID ORGANIC fertilizer of a kind of ecological, environmental protective.Additionally, selenium main and protein bound in Lumbricus body, after enzymolysis, selenium mainly exists with the form of the organics such as selenomethionine in this selenium-enriched earthworm fertilizer, is conducive to the plant leaf blade of corn, vegetable, fruit tree etc. absorb and utilize.In selenium-enriched earthworm fertilizer of the present invention, inorganic selenium content is few, environment will not be had a negative impact, be a kind of environmentally friendly selenium-enriched earthworm fertilizer.Finally, this fertilizer Se content is abundant, amino acid content is high, Acidity of Aikalinity, close to neutral, advantageously absorbs in plant stem-leaf, has promotion crop and fruit and vegerable growth, sterilization, suppresses Metal uptake to accumulate, improve the yield and quality, improve the critical functions such as Se accumulation amount.
Detailed description of the invention
Below in conjunction with embodiment, embodiments of the present invention are described in further detail.Following example are used for illustrating the present invention, but can not be used for limiting the scope of the present invention.
The invention discloses a kind of fertilizer for improving the Se content of crops, vegetable and fruit, yield and quality, be mainly made up of organic selenium, earthworm-amino acid, wormcast extracting solution and various nutrient elements.The present invention is further illustrated below by manufacture method embodiment.
Embodiment 1
Make selenium-enriched earthworm fertilizer and include step: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, cross 4mm sieve respectively, be then sufficiently mixed uniformly, obtain raising the substrate of Lumbricus;Preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, is joined by 0.8L sodium selenite aqueous solution in the substrate of the 20kg weight obtained;In the another substrate that a certain amount of water is joined the other 20kg weight obtained;Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust;The selenium-rich breeding dust obtained and common breeding dust introduce Lumbricus, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 18 days respectively, earthworm breeding is after 45 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm;Selenium-enriched earthworm bowel relieving 24 hours under dark condition that will obtain, then clean with clear water, clean selenium-enriched earthworm tissue refiner are carried out homogenizing homogenate;The multiple enzyme that Lumbricus self contains is utilized to carry out self-dissolving enzymolysis under 55 DEG C of conditions the Lumbricus homogenate obtained, every stirring in 5.5 hours once, enzymolysis 24 hours, obtain selenium-rich amino acid nutrition stock solution;The common wormcast obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5.5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 4 hours, it is filtrated to get wormcast extracting solution, wormcast extracting solution is mixed by 1:1 volume ratio with selenium-rich amino acid nutrition stock solution, by mixed liquor sterilizing 2 hours under 100 DEG C of conditions, then cooling, filtration, obtaining selenium-enriched earthworm fertilizer, its Se content is 10.62mg/L, and wherein organic selenium content is up to 97.32%.
Embodiment 2
Make selenium-enriched earthworm fertilizer and include step: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, cross 3mm sieve respectively, be then sufficiently mixed uniformly, obtain raising the substrate of Lumbricus;Preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, is joined by 0.5L sodium selenite aqueous solution in the substrate of the 12.5kg weight obtained;In the another substrate that a certain amount of water is joined the other 12.5kg weight obtained;Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust;The selenium-rich breeding dust obtained and common breeding dust introduce Lumbricus, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 15 days respectively, earthworm breeding is after 40 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm;Selenium-enriched earthworm bowel relieving 20 hours under dark condition that will obtain, then clean with clear water, clean selenium-enriched earthworm tissue refiner are carried out homogenizing homogenate;The multiple enzyme that Lumbricus self contains is utilized to carry out self-dissolving enzymolysis under 50 DEG C of conditions the Lumbricus homogenate obtained, every stirring in 5 hours once, enzymolysis 20 hours, obtain selenium-rich amino acid nutrition stock solution;The common wormcast obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 3 hours, it is filtrated to get wormcast extracting solution, wormcast extracting solution is mixed by 1:0.8 volume ratio with selenium-rich amino acid nutrition stock solution, by mixed liquor sterilizing 1.5 hours under 100 DEG C of conditions, then cooling, filtration, obtaining selenium-enriched earthworm fertilizer selenium, its Se content is about 8.2mg/L, and wherein organic selenium content accounts for 96%.
Embodiment 3
Make selenium-enriched earthworm fertilizer and include step: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, cross 5mm sieve respectively, be then sufficiently mixed uniformly, obtain raising the substrate of Lumbricus;Preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, is joined by 1.0L sodium selenite aqueous solution in the substrate of the 25kg weight obtained;In the another substrate that a certain amount of water is joined the other 25kg weight obtained;Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust;The selenium-rich breeding dust obtained and common breeding dust introduce Lumbricus, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 20 days respectively, earthworm breeding is after 50 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm;Selenium-enriched earthworm bowel relieving 26 hours under dark condition that will obtain, then clean with clear water, clean selenium-enriched earthworm tissue refiner are carried out homogenizing homogenate;The multiple enzyme that Lumbricus self contains is utilized to carry out self-dissolving enzymolysis under 60 DEG C of conditions the Lumbricus homogenate obtained, every stirring in 6 hours once, enzymolysis 26 hours, obtain selenium-rich amino acid nutrition stock solution;The common wormcast obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:6, it is sufficiently stirred at ambient temperature, it is filtered after stirring 5 hours, it is filtrated to get wormcast extracting solution, wormcast extracting solution is mixed by 1:1.2 volume ratio with selenium-rich amino acid nutrition stock solution, by mixed liquor sterilizing 2.5 hours under 100 DEG C of conditions, then cooling, filtration, obtaining selenium-enriched earthworm fertilizer, its Se content is about 12.1mg/L, and wherein organic selenium content accounts for 97.5%.
Embodiment of above is merely to illustrate the present invention, but not limitation of the present invention.Although the present invention being described in detail with reference to embodiment, it will be understood by those within the art that, technical scheme is carried out various combination, amendment or equivalent replacement, without departure from the spirit and scope of technical solution of the present invention, all should be encompassed in the middle of scope of the presently claimed invention.
Claims (8)
1. the manufacture method of a selenium-enriched earthworm fertilizer, it is characterised in that the method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 3-5mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus;
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins the sodium selenite aqueous solution of 0.5-1.0L in the substrate of the 12.5-25kg weight that step one obtains;In the another substrate that a certain amount of water is joined the other 1.55-25kg weight that step one obtains;Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust;
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raise at 22-28 DEG C of temperature, once identical with former substrate substrate is added respectively every 15-20 days, after earthworm breeding 40-50 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm, standby;
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 20-26 hour under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate, obtains Lumbricus homogenate;
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 50-60 DEG C of condition, every stirring in 5-6 hour once, enzymolysis 20-26 hour, obtain selenium-rich amino acid nutrition stock solution;
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5-6, it is sufficiently stirred at ambient temperature, it is filtered after stirring 3-5 hour, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed according to a certain percentage, by mixed liquor sterilizing 1.5-2.5 hour under 100 DEG C of conditions, then cooling, filtration, to obtain final product.
2. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that described feces of livestock and poultry is cattle manure.
3. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that described dreg is the residue of mushroom culture medium material, it is preferable that the residue of culture medium for golden mushroom material.
4. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that described Lumbricus is girdled adult Eisenia foetida.
5. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that in step 6, described wormcast extracting solution mixes according to volume ratio 1:0.8-1.2 with selenium-rich amino acid nutrition stock solution.
6. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that the method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 4mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus;
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins 0.8L sodium selenite aqueous solution in the substrate of the 20kg weight that step one obtains;In the another substrate that a certain amount of water is joined the other 20kg weight that step one obtains;Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust;
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 18 days respectively, earthworm breeding is after 45 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm;
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 24 hours under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate;
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 55 DEG C of conditions, every stirring in 5.5 hours once, enzymolysis 24 hours, obtain selenium-rich amino acid nutrition stock solution;
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5.5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 4 hours, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed according to volume ratio 1:1, by mixed liquor sterilizing 2 hours under 100 DEG C of conditions, then cooling, filtration, to obtain final product.
7. the selenium-enriched earthworm fertilizer that the manufacture method of the selenium-enriched earthworm fertilizer described in claim 1-6 prepares, it is characterised in that its total Se content is about 8-12mg/L, and wherein organic selenium content accounts for more than 96%.
8. selenium-enriched earthworm fertilizer according to claim 7, it is characterised in that its pH value is 6.8, close to neutral.
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