CN105769941A - Application of Chinese torreya essential oil to preparation of anti-dermatophytes cosmetics and medicine - Google Patents
Application of Chinese torreya essential oil to preparation of anti-dermatophytes cosmetics and medicine Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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Abstract
The invention belongs to the field of natural medicine and cosmetics, and provides application of Chinese torreya essential oil to preparation of anti-dermatophytes medicine, or application of Chinese torreya essential oil as efficiency ingredients in cosmetics for inhibiting the dermatophytes. In-vitro antibacterial activity study on the Chinese torreya essential oil shows that the Chinese torreya essential oil has better antibacterial activity on common pathogenic dermatophytes capable of causing tinea capitis, tinea corporis and little onychomycosis. The Chinese torreya essential oil is essential oil prepared from Chinese torreya aril by a distillation method, a cold squeezing method, a solvent extracting method or a carbon dioxide supercritical extraction method and the like. The Chinese torreya essential oil has the quality requirements that the Chinese torreya essential oil is colourless to yellowish Green liquid, and has the characteristic flavor of Chinese torreya fruit; the relative density (20 DEG C/20 DEG C) is 0.850 to 0.860; the content sum of characteristic components of Alpha-pinene and limonene is greater than or equal to 30 percent. The invention discloses a new purpose of the Chinese torreya essential oil to prepare anti-dermatophytes medicine or cosmetics. In addition, an optimized preparation method of the Chinese torreya essential oil is provided by comparing the advantages and disadvantages of different extraction methods of the Chinese torreya essential oil.
Description
Technical field:
The present invention relates to field of natural medicinal chemistry and cosmetic field, be specifically related to extract for raw material with Chinese torreya aril
Chinese torreya essential oil application in the medicine preparing anti-dermatophytosis and cosmetics.
Background technology:
Chinese torreya (Torreya grandis) has another name called Semen Torreyae, Cornu Caprae seu Ovis Chinese torreya, thin Chinese torreya etc., is that taxaceae (Taxaceae) Chinese torreya belongs to
Aiphyllium, is belonged to kind of an interior natural variation type by Chinese torreya and cultivates and next improved seeds through asexual propagation, be distributed in China
Zhejiang, Jiangsu, Anhui, the province such as Fujian, main producing region is the Zhuji in Guiji Shan Mountain district of Zhejiang Province, Shengzhou, Shaoxing, east
The ground such as sun, Pan'an.Chinese torreya is to integrate use edible, medicinal, oily, material use and view and admire the multipurpose rare tree of greening.
The fruit Chinese torreya fruit of Chinese torreya enjoys favor and certainly the most always, but account for its seed fresh weight 50～60% outer covering of the seed thicker
Meat aril part is not the most effectively utilized, and is taken as processing waste to be deposited in Around the house and streams for a long time
Limit, not only causes the wasting of resources, also pollutes environment.How to develop Chinese torreya aril, prevent it from causing environmental pollution,
Always Chinese torreya industry is paid close attention to and problem demanding prompt solution.Recent studies indicate that, Chinese torreya aril rich in diterpene, wave
The bioactive ingredients such as hair oil, flavone compound, but Chinese torreya aril is the most well studied and is utilized.
Quintessence oil, also known as volatile oil, aromatic oil, is that the class that fragrant plant contains has volatility and fat-soluble oily mater.
Chemical Composition of The Essential Oil is more complicated, can be divided into terpenoid, aromatic compound, aliphatic compound by structure, be contained
Sulfur nitrogen-containing heterocycle compound, there is the special compound of huge ring lactone structure.Quintessence oil has multiple physiologically active, including antibacterial,
Antiinflammatory, antiviral, analgesia, antitumor, mosquito dispersing, rush infiltration, antioxidation etc..Chinese torreya aril, rich in quintessence oil, is
Its contained important secondary metabolite of class, also lacks relevant researches and exploitation at present.Therefore, in order to find
The new application of Chinese torreya essential oil, the present invention has carried out relevant research.Prior art having no, Chinese torreya essential oil is at anti-dermatophytosis
The application of aspect.
Summary of the invention:
It is an object of the invention to provide Chinese torreya essential oil application in the cosmetics preparing anti-dermatophytosis or medicine.In order to
Realize the above-mentioned purpose of the present invention, the invention provides following technical scheme:
Chinese torreya essential oil application in the medicine preparing anti-dermatophytosis.
Chinese torreya essential oil application in the cosmetics of preparation suppression dermatophytosis.
Applying as mentioned, wherein said tinea is tinea capitis, tinea corporis and tinea unguium.
Applying as mentioned, wherein said dermatophytosis is Common Pathogenic dermatophytosis.
Applying as mentioned, wherein said Chinese torreya essential oil is colourless to pistac liquid, and the feature with Chinese torreya fruit is fragrant
Gas, relative density 20 DEG C/20 DEG C is 0.850～0.860, characteristic component australene and content sum >=30% of limonene.
Apply as mentioned, wherein said Chinese torreya essential oil be from the Chinese torreya of the Chinese torreya fruit aril way of distillation or cold press method or
Solvent extraction method or the preparation-obtained quintessence oil of carbon dioxide supercritical extraction method.
Applying as mentioned, wherein said Chinese torreya essential oil is that the Chinese torreya fruit aril way of distillation from Chinese torreya extracts Chinese torreya essential oil,
Dry Chinese torreya aril is the Chinese torreya crust of natural air drying, and size-reduced machine is crushed to 80～100 mesh, adds the water logging from the beginning of 1: 6
Steeping 6 hours, water distillation 2～4 hours, distillate separates to obtain the quintessence oil of dry Chinese torreya crust, dry Chinese torreya through oil water separator again
It is 1.0～1.3%w/w that aril extracts the oil yield of quintessence oil, dry basis.
Applying as mentioned, wherein said Chinese torreya essential oil is that the Chinese torreya fruit aril way of distillation from Chinese torreya is extracted or cold press
Method extracts fresh Chinese torreya aril quintessence oil, and described fresh Chinese torreya aril refers to green less than 1 day of room temperature lower resting period
Color Chinese torreya crust, fresh Chinese torreya crust is cut little after add 2～3 times of water carries out water distillation 2～4 hours；Or use screw rod cold
Crusher squeeze juice, after gained juice machine by centrifugation is centrifuged precipitation, centrifuge speed is 5000 revs/min, and the time is
20 minutes, take supernatant and distill, then the quintessence oil of fresh Chinese torreya aril can be obtained through oil water separator separation distillate,
It is 0.35～0.45%w/w from the oil yield of the Chinese torreya essential oil of fresh Chinese torreya aril gained, wet basis weight.
Present invention also offers a kind of compositions for anti-dermatophytosis, said composition comprises as sole active agent
Have following speciality colourless to pistac liquid, have Chinese torreya fruit characteristic perfume, relative density 20 DEG C/20 DEG C be
0.850～0.860, the Chinese torreya essential oil of content sum >=30% of characteristic component australene and limonene.
The application in the cosmetics preparing anti-dermatophytosis or medicine of the compositions of described anti-dermatophytosis, wherein combines
Composition Chinese torreya essential oil in thing is that the Chinese torreya fruit aril way of distillation from Chinese torreya extracts Chinese torreya essential oil, the Chinese torreya fruit aril of Chinese torreya
Including dry Chinese torreya fruit aril or fresh Chinese torreya fruit aril, it is 1.0～1.3% that dry Chinese torreya aril extracts the oil yield of quintessence oil
W/w, dry basis, the oil yield of the Chinese torreya essential oil that fresh Chinese torreya aril extracts gained is 0.35～0.45%w/w, wet basis weight,
During use, said composition is uniformly applied to outside baring skin.
The technical problem to be solved in the present invention is to provide the purposes of Chinese torreya essential oil, i.e. application in anti-tinea microbial inoculum.
In order to solve above-mentioned technical problem, the present invention provides Chinese torreya essential oil application in anti-dermatophytosis medicine and cosmetics.
Improvement in the application in anti-tinea microbial inoculum of the Chinese torreya essential oil of the present invention, Chinese torreya essential oil is by Chinese torreya fruit aril
Extract the quintessence oil obtained.Chinese torreya essential oil is by the way of distillation, cold press method, solvent extraction method or dioxy from Chinese torreya aril
Change the quintessence oil obtained by the preparations such as carbon supercritical extraction.Chinese torreya essential oil obtained by preferably being prepared by the way of distillation.Chinese torreya
The characteristic chemical constituent of quintessence oil is: α pinene, limonene etc., and content >=30 of both sums.Chinese torreya essential oil can be used alone
Also can compounding with other anti-dermatophytosis effective ingredient use.During use, can directly spread upon on skin.
Accompanying drawing illustrates:
Fig. 1 is Chinese torreya essential oil Typical gas chromatograph figure.
Detailed description of the invention:
Below in conjunction with the accompanying drawings, further illustrate the essentiality content of the present invention with embodiments of the invention, but not with this
Limit the present invention.
Embodiment 1:
The way of distillation, the cold press method fresh Chinese torreya aril quintessence oil of extraction:
With the aril of fresh Chinese torreya (Torreya grandis cv Meorillii) fruit as raw material, do not surpass for the room temperature lower resting period
Cross the green Chinese torreya crust of 1 day, from fresh Chinese torreya aril extract quintessence oil can first crust be cut little after add 2～3 times of water are carried out
Water distillation 2～the method for 4 hours, or use screw rod cold pressing expeller squeeze juice, gained juice machine by centrifugation is centrifuged sinking
Behind shallow lake (centrifuge speed is 5000 revs/min, and the time is 20 minutes), take supernatant and distill, then through oil-water separation
Device separation distillate can obtain the quintessence oil of fresh Chinese torreya aril.Fuel-displaced from the Chinese torreya essential oil of fresh Chinese torreya aril gained
Rate is 0.35～0.45% (w/w, wet basis weight).
CO2 supercritical extraction fresh Chinese torreya aril quintessence oil:
300g fresh Chinese torreya aril is chopped into 5 × 5mm or mashes, and extraction temperature is 45 DEG C, and extracting pressure is
15 or 20MP, extraction time is 120 minutes, and yield is 0.05～0.3% (w/w, wet basis weight).Extraction equipment is the U.S.
Produce Applied Separations supercritical carbon dioxide extraction instrument.
The way of distillation dry Chinese torreya aril quintessence oil of extraction:
Dry Chinese torreya aril refers to the Chinese torreya crust of natural air drying, and size-reduced machine is crushed to 80～100 mesh, adds 1:6 oneself
Water carries out soaking 6 hours, and carries out water distillation 2～4 hours, distillate again through oil water separator separate can obtain dry
The quintessence oil of Chinese torreya crust.It is 1.0～1.3% (w/w, dry basis) that dry Chinese torreya aril extracts the oil yield of quintessence oil.
The solvent extraction method dry Chinese torreya aril quintessence oil of extraction:
The Chinese torreya crust of natural air drying, size-reduced machine is crushed to 80～100 mesh, add 1:10 organic solvent (petroleum ether,
Normal hexane or ether etc.) merceration or by heating and refluxing extraction, the most available Chinese torreya extractum after reclaiming organic solvent.Extraction ratio
It is 5% (w/w, dry basis).
The chemical composition of the Chinese torreya essential oil that above extracting method obtains is compared research, uses U.S. Agilent
Technologies company 7890GC 5975MS gas chromatograph-mass spectrometer (GC MS), HP 5MS quartz capillary
Each essential oil smaple is analyzed by chromatographic column, and identifies main component therein.Main component therein is similar to, but respectively
Composition ratio shared by quintessence oil is had any different, and what content was the highest is α pinene and limonene.Supercritical carbon dioxide extracts
The technology that takes is far above the way of distillation on running cost, and the Chinese torreya essential oil obtained by supercritical carbon dioxide extraction is in yield
Do not improve, abnormal smells from the patient is also without significantly improving, and contains more involatile constituent.Solvent extraction method also has cost high
It is difficult to the shortcomings such as operation.Therefore extracting the preferable method of Chinese torreya essential oil is to use traditional way of distillation to extract.
Embodiment 2:
The present invention use fragrant torreya essential oil as the functional component of anti-dermatophytosis product, for confirming Chinese torreya of the present invention
Quintessence oil effect on anti-dermatophytosis, the quintessence oil obtained from the distillation of Chinese torreya aril has been carried out anti-dermatophytosis by the present invention
The research of activity, concrete method and result are as follows:
1. experimental strain
The antibacterial activity in vitro research bacterial strain uses therefor of Chinese torreya essential oil is primarily directed to cause the normal of tinea capitis, tinea corporis and minority tinea unguium
The 6 kinds of pathogenic dermatophytosises seen: Sabouraudites lanosus, microsporon gypseum, Microsporum ferrugineum, Ai Ze building hair
Tinea bacterium, alpha fungus, acrothesium floccosum.
2. experimental technique
The test of minimal inhibitory concentration MIC be slightly revise according to clinical and laboratory standard file after, enterprising at 96 microwell plates
Row measures, and fungal culture uses Sharpe fluid medium (SDB).The microorganism of inoculation is from the old training having cultivated 10 days
Support prepared by thing, and the concentration of suspension regulates to 0.5 Maxwell turbidity standard (McFarland standard turbidity).
The inoculation of standard is diluted reaching last inoculum density and is of about 2x 105CFU/ml.By etc. test sample be dissolved in 10% 2
Methyl sulfoxide (DMSO) is configured to the solution that initial concentration is 1mg/ml, makes the denseest of sample by serial dilution
Degree scope is 1,000 7.8 μ g/ml.In each experiment, all include following test: 10%v/v DMSO aqueous solution and
Culture medium, negative control (10%v/v DMSO aqueous solution and culture medium are plus inoculum), positive control (10%v/v DMSO
Aqueous solution and culture medium, plus inoculum, add positive control medicine).In 28 DEG C after 96 microwell plates of experiment are added a cover
Observed result after cultivating 5 days.
3. experimental result
The minimal inhibitory concentration (MIC) of the anti-dermatophytosis of Chinese torreya essential oil is summarized in table 2.1, and the result of activity research shows:
Chinese torreya essential oil has inhibitory action in various degree to 6 strain Common Pathogenic dermatophytosises used by this experiment, and its MIC is situated between
Between 125 μ g/ml to 500 μ g/ml.Therefore, Chinese torreya essential oil has preferable bacteriostatic activity to tested dermatophytosis.
Table 1 Chinese torreya essential oil anti-dermatophytosis active testing
Embodiment 3:
Preparation method: add a kind of composition under stirring in the double distilled water of proper volume every time, solve, so until the deepest
After add another kind of composition.Add double distilled water to after 2ml, this solution is filtered on sterilizing filter, loads in bottle
And separate according to suitable dosage.
Embodiment 4:
Skin whitener formula (W%) containing Chinese torreya essential oil:
The method making cosmetics routinely prepares the cosmetics of the above-mentioned formula of the present invention.
Embodiment 5:
Emulsion formulations (W%) containing Chinese torreya essential oil:
The method making cosmetics routinely prepares the cosmetics of the above-mentioned formula of the present invention.
Embodiment 6:
Astringent:
Aqueous phase: sodium polyacrylate 0.35 gram, glycerol 4 grams, 1,3 butanediol 2.5 grams, Radix Hamamelidis Mollis extracting solution 2.5 grams,
Vitamin B5 0.5 gram, albutin 0.5 gram, EDTA Na2 50mg；Deionized water, in right amount, adds to 100ml.System
Preparation Method: sodium polyacrylate 0.3 gram is dissolved in 70ml water, stirring, the most swelling.It is slowly added in prescription remaining
Point, do not stop stirring；Being subsequently adding the Chinese torreya essential oil of 0.2%, stirring makes it become homogeneous.Deionized water, adds to 100ml,
Pack.
Embodiment 7:
Repair cream:
Prescription: glycerol 6 grams, carbomer 1.5 grams, triethanolamine 1.5 grams, propylene glycol 6 grams, Ethyl Hydroxybenzoate 0.2
Gram, tanshinone extract 1.2 grams, Chinese torreya essential oil, deionized water is appropriate, and adding ad pond om is 100 grams.Preparation method:
Take carbomer in appropriate amount of deionized water, stir evenly, stand overnight so that it is after the most swelling, after adding glycerol, use three second
Amine regulation pH value, makes gel-type vehicle toughness increase.Separately take the tanshinone extract of recipe quantity, propylene glycol and deionized water,
Mix homogeneously.Again carbomer gel example is entered, add 0.5% Chinese torreya essential oil, Ethyl Hydroxybenzoate, merge and be stirred until homogeneous carefully
Greasy, add distilled water the most enough, grind uniformly,.
Aforesaid explanation should be understood to only illustrate the present invention.Various selections and correction can by one skilled in the art not
Deviate from the present invention and design.Thus, it is intended that include all this selections fallen within the scope of the following claims and
Revise and change.
Claims (10)
1. Chinese torreya essential oil application in the medicine preparing anti-dermatophytosis.
2. Chinese torreya essential oil application in the cosmetics of preparation suppression dermatophytosis.
Apply the most as claimed in claim 1 or 2, it is characterised in that described tinea is tinea capitis, tinea corporis, tinea unguium.
Apply the most as claimed in claim 1 or 2, it is characterised in that described dermatophytosis is Common Pathogenic tinea
Bacterium.
Apply the most as claimed in claim 1 or 2, it is characterised in that described Chinese torreya essential oil is colourless to pistac liquid
Body, has the characteristic perfume of Chinese torreya fruit, and relative density 20 DEG C/20 DEG C is 0.850～0.860, characteristic component australene and lemon
Content sum >=30% of lemon alkene.
Apply the most as claimed in claim 1 or 2, it is characterised in that described Chinese torreya essential oil is the false kind of Chinese torreya fruit from Chinese torreya
The skin way of distillation or cold press method or solvent extraction method or the preparation-obtained quintessence oil of carbon dioxide supercritical extraction method, or symbol
Close the Chinese torreya essential oil that arbitrary extracting method of the above Chinese torreya essential oil quality requirements obtains.
Apply the most as claimed in claim 1 or 2, it is characterised in that described Chinese torreya essential oil is the Chinese torreya fruit aril from Chinese torreya
Extracting Chinese torreya essential oil by the way of distillation, dry Chinese torreya aril is the Chinese torreya crust of natural air drying, and size-reduced machine is crushed to 80～100
Mesh, the tap water adding 1: 6 soaks 6 hours, and water distillation 2～4 hours, distillate separates dry through oil water separator again
The quintessence oil of Chinese torreya crust, it is 1.0～1.3%w/w that dry Chinese torreya aril extracts the oil yield of quintessence oil, dry basis.
Apply the most as claimed in claim 1 or 2, it is characterised in that described Chinese torreya essential oil is the false kind of Chinese torreya fruit from Chinese torreya
The skin way of distillation is extracted or cold press method extracts fresh Chinese torreya aril quintessence oil, and described fresh Chinese torreya aril refers under room temperature
Resting period less than the green Chinese torreya crust of 1 day, fresh Chinese torreya crust is cut little after add 2～3 times of water carry out water distillation
2～4 hours；Or use screw rod cold pressing expeller squeeze juice, and after gained juice machine by centrifugation is centrifuged precipitation, centrifuge speed
Being 5000 revs/min, the time is 20 minutes, takes supernatant and distills, then can obtain through oil water separator separation distillate
The quintessence oil of fresh Chinese torreya aril, is 0.35～0.45%w/w from the oil yield of the Chinese torreya essential oil of fresh Chinese torreya aril gained,
Wet basis weight.
9., for the compositions of anti-dermatophytosis, said composition comprises and has following speciality as sole active agent
Colourless to pistac liquid, there is the characteristic perfume of Chinese torreya fruit, relative density 20 DEG C/20 DEG C be 0.850～0.860,
The Chinese torreya essential oil of content sum >=30% of characteristic component australene and limonene.
10. the compositions of the anti-dermatophytosis described in claim 9 is in the cosmetics preparing anti-dermatophytosis or medicine
Application, it is characterised in that wherein the composition Chinese torreya essential oil in compositions is that the Chinese torreya fruit aril way of distillation from Chinese torreya extracts perfume
Chinese torreya quintessence oil, the Chinese torreya fruit aril of Chinese torreya includes dry Chinese torreya fruit aril or fresh Chinese torreya fruit aril, and dry Chinese torreya aril is extracted
The oil yield of quintessence oil is 1.0～1.3%w/w, dry basis, and fresh Chinese torreya aril extracts the oil yield of the Chinese torreya essential oil of gained
It is 0.35～0.45%w/w, wet basis weight, during use, said composition is uniformly applied to outside baring skin.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106883959A (en) * | 2017-02-24 | 2017-06-23 | 浙江如美日化有限公司 | A kind of Chinese torreya essential oil soap |
| CN109431867A (en) * | 2018-12-05 | 2019-03-08 | 张玉婷 | One kind is dispelled blackhead massage oil |
| CN113197798A (en) * | 2021-05-10 | 2021-08-03 | 上海应用技术大学 | Plant hydrolat with antioxidant and anti-aging effects and preparation method and application thereof |
| CN113244433A (en) * | 2021-06-18 | 2021-08-13 | 宁波华木来生物科技有限公司 | Preparation method of bactericidal essential oil with fruit fragrance |
| KR20220049675A (en) * | 2020-10-14 | 2022-04-22 | 주식회사 현대바이오랜드 | Cosmetic composition comprising extracts of burdock seed and Torreya grandis aril for improving wrinkles, elasticity or moisturizing the skin |
| CN114989895A (en) * | 2022-07-07 | 2022-09-02 | 浙大宁波理工学院 | Preparation method of torreya grandis epicarp volatile oil with antibacterial activity, and product and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106883959A (en) * | 2017-02-24 | 2017-06-23 | 浙江如美日化有限公司 | A kind of Chinese torreya essential oil soap |
| CN109431867A (en) * | 2018-12-05 | 2019-03-08 | 张玉婷 | One kind is dispelled blackhead massage oil |
| KR20220049675A (en) * | 2020-10-14 | 2022-04-22 | 주식회사 현대바이오랜드 | Cosmetic composition comprising extracts of burdock seed and Torreya grandis aril for improving wrinkles, elasticity or moisturizing the skin |
| CN113197798A (en) * | 2021-05-10 | 2021-08-03 | 上海应用技术大学 | Plant hydrolat with antioxidant and anti-aging effects and preparation method and application thereof |
| CN113244433A (en) * | 2021-06-18 | 2021-08-13 | 宁波华木来生物科技有限公司 | Preparation method of bactericidal essential oil with fruit fragrance |
| CN114989895A (en) * | 2022-07-07 | 2022-09-02 | 浙大宁波理工学院 | Preparation method of torreya grandis epicarp volatile oil with antibacterial activity, and product and application thereof |
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| CN105769941B (en) | 2020-08-04 |
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