CN105769653B - A kind of activity stabilized SOD skin care compositions and preparation method thereof - Google Patents
A kind of activity stabilized SOD skin care compositions and preparation method thereof Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/08—Anti-ageing preparations
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Abstract
The present invention provides a kind of activity stabilized SOD skin care compositions and preparation method thereof, concrete operation step are as follows: (1) choose fresh Aloe leave and garlic leaf, after the impurity for removing surface, it is cut into small pieces, ultrasonication in phosphate buffer is added, uniform solution is stirred to get, supernatant is taken to obtain thick enzyme filtrate after centrifuge separation；(2) thick enzyme filtrate prepared by step (1) is heated to 30-35 DEG C, mannitol and sucrose is added, is stirred to react 20-30min, after cooling rapidly, after ultrafiltration membrance filter, the SOD enzyme solution modified；(3) it by after the SOD enzyme solution high-temperature sterilization of the modification of step (2) preparation, is added in natural skin care composition, stirs evenly, obtain activity stabilized SOD skin care compositions.The preparation method of activity stabilized SOD skin care compositions prepared by the present invention is easy, and the SOD for combining preparation with chemical modification gimmick using physics is activity stabilized, can provide effective component for a long time, achieve the purpose that lasting skin care.
Description
Technical field
The invention belongs to amenities technical fields, and in particular to a kind of activity stabilized SOD skin care compositions and its system
Preparation Method.
Background technique
With the continuous improvement of living standards, requirement of the people to cosmetics is also increasingly toward high-level development, Ren Menyi
Through being unsatisfactory for simple skin-protection product, it is also necessary to have the skin-protection product of specific function, such as dispelling stain prevents wrinkle, keeps flesh
Skin elasticity etc..
Superoxide dismutase SOD belongs to metalloenzyme, is present in animal, plant, algae and other prokaryotes bodies more
In, the amino acid sequence slightly difference for the purifying enzyme that separate sources extracts, therefore property also difference.The property of SOD is not only
Depending on protein portion, and the metal ion of bonding agent active site is depended on, according to the type of the metal ion of combination
Difference is divided into copper zinc SOD, iron SOD and manganese SOD, and superoxide anion catalytic disproportionation can be hydrogen peroxide and oxygen by they.
According to the free-radical theory of skin aging, in the aging course of skin, damage of the free radical to skin is one
Important factor, the free radical generated in vivo and fatty acid act on forming the substances such as malonaldehyde, and malonaldehyde is equal on cell membrane
The effects of protein, forms lipofuscin and is deposited on skin, forms a variety of color spots, and free radical can also make collagen in epidermis fine
Dimension, elastic fibers crosslinking and denaturation become fragile and follow the string.When the water deficient of skin, it is easy to produce wrinkle.Therefore freely
Base be for the detrimental effect of skin it is very big, need to effectively eliminate the free radical generated, and reduce new
The generation of free radical, and the moisture of skin is kept, it thus can effectively slow down the aging of skin.
SOD is effective scavenger of superoxide anion free radical, but the SOD deposited at present is easy inactivation.Chinese patent
A kind of SOD modification production method, the SOD enzyme that will be extracted from animal blood disclosed in CN101768582B (publication date 2010.7.7)
Liquid adds the environment and process of the chemical reaction of lauroyl chloride and aqueous slkali modification SOD, improves the active stability of SOD, and
Traditional acetone is not added, ensure that the safety of product and the activity of SOD.Chinese patent CN102747054B (publication date
2012.10.24 a kind of extracting method of SOD disclosed in) is centrifuged to obtain SOD from the leaching liquor of Rosa roxburghii Tratt fruit powder thick
Enzyme solution obtains SOD solution through ultrafiltration, then condenses centrifugal spray drying and obtain SOD.This method whole process uses low-temperature physics gimmick,
It ensure that bioactivity, and there is no environmental pollution and chemical contamination.Have above-mentioned and has technology it is found that in order to improve SOD activity
Stability, can perhaps chemical gimmick carries out but pure chemistry or physics gimmick modification SOD is used to have using physics
Certain limitation, therefore, the present invention is using physics and chemical gimmick and combines modified SOD, obtains activity stabilized SOD skin care
Product.
Summary of the invention:
The technical problem to be solved in the present invention is to provide a kind of activity stabilized SOD skin care compositions and preparation method thereof,
The crude enzyme liquid that will be extracted from fresh garlic and aloe obtains activity stabilized SOD after the modification purification of chemically and physically method,
SOD is introduced into skin care compositions again, obtains activity stabilized SOD skin care compositions.
In order to solve the above technical problems, the technical scheme is that
A kind of activity stabilized SOD skin care compositions, which is characterized in that include modification in the SOD skin care compositions
SOD, the SOD of the modification are the SOD through activity stabilized modification, are also combined comprising natural skin care in the SOD skin care compositions
Object, the auxiliary agent also extracted comprising natural animal-plant in the natural skin care composition, the auxiliary agent include moisturizer, emollient,
Preservative and dispersing agent.
Preferably, the moisturizer is silk peptide, and skin conditioner is lanolin, and the preservative is tea polyphenols, and dispersing agent is big
Legumin surfactant.
The present invention also provides a kind of preparation methods of activity stabilized SOD skin care compositions, it is characterised in that: including following
Step:
(1) it chooses fresh Aloe leave and garlic leaf, after the impurity for removing surface, is cut into small pieces, it is slow that phosphoric acid is added
Ultrasonication in fliud flushing stirs to get uniform solution, and supernatant is taken to obtain thick enzyme filtrate after centrifuge separation；
(2) thick enzyme filtrate prepared by step (1) is heated to 30-35 DEG C, mannitol and sucrose is added, is stirred to react 20-
30min, after cooling rapidly, after ultrafiltration membrance filter, the SOD enzyme solution modified；
(3) it by after the SOD enzyme solution high-temperature sterilization of the modification of step (2) preparation, is added in natural skin care composition, stirring is equal
It is even, obtain activity stabilized SOD skin care compositions.
As a preferred embodiment of the above technical solution, in the step (1), the mass ratio of Aloe leave and garlic leaf is 1:
0.1-10。
As a preferred embodiment of the above technical solution, in the step (1), the concentration of phosphate buffer is 0.06-0.08mol/
L, pH value 7.8.
As a preferred embodiment of the above technical solution, in the step (1), the power of ultrasonic wave is 250-300W, ultrasonic time
For 3-5min.
As a preferred embodiment of the above technical solution, in the step (2), thick enzyme filtrate and the mass ratio of mannitol and sucrose are
50:1-2:0.5-2.
As a preferred embodiment of the above technical solution, in the step (2), the condition of ultrafiltration membrance filter is 30 DEG C, pressure
1.5MPa, transmitance 85-92%.
As a preferred embodiment of the above technical solution, in the step (3), the content of SOD enzyme is 30- in SOD skin care compositions
50%.
Preferably, the activity stabilized acceptable carrier of SOD skin care compositions is prepared into lotion or face cream.
With the prior art with than, the invention has the following advantages:
(1) main functional component in activity stabilized SOD skin care compositions prepared by the present invention is the SOD of modification, is repaired
The preparation method of the SOD of decorations is easy, activity stabilized using physics and chemical modification gimmick and the SOD of combination preparation, can be for a long time
Effective component is provided, achievees the purpose that lasting skin care.
(2) SOD of activity modifying prepared by the present invention selects phosphate buffer extraction and ultrasonication technology and combines
Gimmick extract the thick enzyme of SOD, can quickly and effectively increase the release, diffusion and dissolution of intracellular matter, it is high to obtain recovery rate
Then SOD effective component handles via the chemical stabilization of mannitol and sucrose, obtains activity stabilized SOD.
(3) auxiliary agent extracted in natural animal-plant has also been selected in SOD skincare composition prepared by the present invention, has not selected people
The chemical substance of work synthesis guarantees integral product green natural, and environmental protection is effectively.
Specific embodiment:
Below in conjunction with specific embodiment, the present invention will be described in detail, herein illustrative examples and explanation of the invention
For explaining the present invention, but it is not as a limitation of the invention.
Embodiment 1:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:0.1, after the impurity for removing surface, is cut into
The pH value of 0.06mol/L is added as in 7.8 phosphate buffer, 250W ultrasonication 3min is stirred to get uniform in fritter
Solution takes supernatant to obtain the thick enzyme filtrate of 2.3mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 30 DEG C, 1 part of mannitol and 0.5 part of sugarcane is added
Sugar is stirred to react 20min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, obtains transmitance
For the SOD enzyme solution of 85% modification.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 30%.
Embodiment 2:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:10, after the impurity for removing surface, is cut into small
The pH value of 0.08mol/L is added as in 7.8 phosphate buffer, 300W ultrasonication 5min is stirred to get uniform molten in block
Liquid takes supernatant to obtain the thick enzyme filtrate of 3.4mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 35 DEG C, 2 parts of mannitol and 2 parts of sucrose is added,
It is stirred to react 30min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, obtaining transmitance is
The SOD enzyme solution of 92% modification.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 50%.
Embodiment 3:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:5, after the impurity for removing surface, is cut into small
The pH value of 0.07mol/L is added as in 7.8 phosphate buffer, 250W ultrasonication 4min is stirred to get uniform molten in block
Liquid takes supernatant to obtain the thick enzyme filtrate of 2.7mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 30-35 DEG C, be added 1.5 parts mannitol and 1 part
Sucrose is stirred to react 25min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, is penetrated
The SOD enzyme solution for the modification that rate is 90%.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 35%.
Embodiment 4:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:1, after the impurity for removing surface, is cut into small
The pH value of 0.06mol/L is added as in 7.8 phosphate buffer, 300W ultrasonication 4min is stirred to get uniform molten in block
Liquid takes supernatant to obtain the thick enzyme filtrate of 3.2mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 30 DEG C, 1 part of mannitol and 1.5 parts of sugarcane is added
Sugar is stirred to react 20min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, obtains transmitance
For the SOD enzyme solution of 88% modification.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 40%.
Embodiment 5:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:3, after the impurity for removing surface, is cut into small
The pH value of 0.08mol/L is added as in 7.8 phosphate buffer, 300W ultrasonication 3min is stirred to get uniform molten in block
Liquid takes supernatant to obtain the thick enzyme filtrate of 2.7mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 35 DEG C, 1.2 parts of mannitol and 1 part of sugarcane is added
Sugar is stirred to react 20min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, obtains transmitance
For the SOD enzyme solution of 90% modification.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 45%.
Embodiment 6:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:0.2, after the impurity for removing surface, is cut into
The pH value of 0.06mol/L is added as in 7.8 phosphate buffer, 250W ultrasonication 3.5min is stirred to get uniformly in fritter
Solution, take supernatant to obtain the thick enzyme filtrate of 2.5mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 30 DEG C, 1.4 parts of mannitol and 0.5 part of sugarcane is added
Sugar is stirred to react 20min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, obtains transmitance
For the SOD enzyme solution of 88% modification.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 38%.
Embodiment 7:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:0.6, after the impurity for removing surface, is cut into
The pH value of 0.07mol/L is added as in 7.8 phosphate buffer, 250W ultrasonication 3.5min is stirred to get uniformly in fritter
Solution, take supernatant to obtain the thick enzyme filtrate of 2.3mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 30 DEG C, 1.6 parts of mannitol and 0.6 part of sugarcane is added
Sugar is stirred to react 20min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, obtains transmitance
For the SOD enzyme solution of 89% modification.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 42%.
Embodiment 8:
(1) mass ratio is chosen as the fresh Aloe leave and garlic leaf of 1:0.8, after the impurity for removing surface, is cut into
The pH value of 0.08mol/L is added as in 7.8 phosphate buffer, 250W ultrasonication 3.5min is stirred to get uniformly in fritter
Solution, take supernatant to obtain the thick enzyme filtrate of 2.9mg/ml after centrifuge separation.
(2) by weight, 50 parts of thick enzyme filtrate is heated to 30 DEG C, 1.9 parts of mannitol and 0.8 part of sugarcane is added
Sugar is stirred to react 20min, rapidly after cooling, under 30 DEG C and 1.5MPa of pressure, after ultrafiltration membrance filter, obtains transmitance
For the SOD enzyme solution of 92% modification.
(3) moisturizer silk peptide, skin conditioner lanolin, preservative tea polyphenols, dispersing agent soybean protein surfactant are mixed
After closing uniformly, the SOD enzyme solution of the modification of high-temperature sterilization is added, stirs evenly, obtains activity stabilized SOD skin care compositions,
The content of middle SOD enzyme is 47%.
Enzyme activity through detecting, after 0h, 1h and 4h of the activity stabilized SOD skin care compositions of embodiment 1-8 preparation
As a result as follows:
As seen from the above table, the enzyme activity of activity stabilized SOD skin care compositions prepared by the present invention is high, and long-time enzyme activity
Power is stablized, and can keep activity for a long time, effectively moisten and protect skin.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (7)
1. a kind of activity stabilized SOD skin care compositions, which is characterized in that include modification in the SOD skin care compositions
SOD, the SOD of the modification are the SOD through activity stabilized modification, are also combined comprising natural skin care in the SOD skin care compositions
Object, the auxiliary agent also extracted comprising natural animal-plant in the natural skin care composition, the auxiliary agent include moisturizer, emollient,
Preservative and dispersing agent, the moisturizer are silk peptide, and emollient is lanolin, and the preservative is tea polyphenols, and dispersing agent is big
Legumin surfactant；
Wherein, the preparation method of the activity stabilized SOD skin care compositions, comprising the following steps:
(1) it chooses fresh Aloe leave and garlic leaf, after the impurity for removing surface, is cut into small pieces, phosphate buffer is added
Middle ultrasonication stirs to get uniform solution, and supernatant is taken to obtain thick enzyme filtrate after centrifuge separation；
(2) thick enzyme filtrate prepared by step (1) is heated to 30-35 DEG C, mannitol and sucrose is added, is stirred to react 20-
30min, after cooling rapidly, after ultrafiltration membrance filter, the SOD enzyme solution modified；
(3) it by after the SOD enzyme solution high-temperature sterilization of the modification of step (2) preparation, is added in natural skin care composition, stirs evenly,
Obtain activity stabilized SOD skin care compositions.
2. the preparation method of the activity stabilized SOD skin care compositions of one kind described in claim 1, it is characterised in that: including with
Lower step:
(1) it chooses fresh Aloe leave and garlic leaf, after the impurity for removing surface, is cut into small pieces, phosphate buffer is added
Middle ultrasonication stirs to get uniform solution, and supernatant is taken to obtain thick enzyme filtrate after centrifuge separation；
(2) thick enzyme filtrate prepared by step (1) is heated to 30-35 DEG C, mannitol and sucrose is added, is stirred to react 20-
30min, after cooling rapidly, after ultrafiltration membrance filter, the SOD enzyme solution modified；
(3) it by after the SOD enzyme solution high-temperature sterilization of the modification of step (2) preparation, is added in natural skin care composition, stirs evenly,
Obtain activity stabilized SOD skin care compositions.
3. the preparation method of the activity stabilized SOD skin care compositions of one kind according to claim 2, it is characterised in that: institute
It states in step (1), the mass ratio of Aloe leave and garlic leaf is 1:0.1-10.
4. the preparation method of the activity stabilized SOD skin care compositions of one kind according to claim 2, it is characterised in that: institute
It states in step (1), the concentration of phosphate buffer is 0.06-0.08mol/L, pH value 7.8.
5. the preparation method of the activity stabilized SOD skin care compositions of one kind according to claim 2, it is characterised in that: institute
It states in step (1), the power of ultrasonic wave is 250-300W, ultrasonic time 3-5min.
6. the preparation method of the activity stabilized SOD skin care compositions of one kind according to claim 2, it is characterised in that: institute
It states in step (2), thick enzyme filtrate is 50:1-2:0.5-2 with the mass ratio of mannitol and sucrose.
7. the preparation method of the activity stabilized SOD skin care compositions of one kind according to claim 2, it is characterised in that: institute
It states in step (2), the condition of ultrafiltration membrance filter is 30 DEG C, pressure 1.5MPa, transmitance 85-92%.
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| CN107653232A (en) * | 2017-11-14 | 2018-02-02 | 合肥创想能源环境科技有限公司 | A kind of technique of ultrafiltration purifying concentration garlic superoxide dismutase |
| CN107951820A (en) * | 2017-12-01 | 2018-04-24 | 重庆金时代生物技术有限公司 | A kind of stem cell skin care composition and preparation method thereof |
| CN108498360A (en) * | 2018-06-05 | 2018-09-07 | 广州诺德丽诗化妆品有限公司 | A kind of anti-apolexis composition and preparation method thereof applied to cosmetics |
| CN108434010B (en) * | 2018-06-06 | 2020-10-20 | 西南交通大学 | Pickering emulsion type cosmetic with stable SOD enzyme particles and preparation process thereof |
| CN112932992A (en) * | 2021-03-19 | 2021-06-11 | 徐晓芬 | A skin care composition for improving skin aging and skin elasticity |
| CN117126822B (en) * | 2023-10-27 | 2023-12-22 | 山东爱维德生物科技有限公司 | Adamantine modified SOD enzyme for arborescent aloe SOD composite pure dew and application thereof |
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| 三种芦荟超氧化物歧化酶的活性测定;董长颖等;《北方园艺》;20091231(第5期);第97页"1.2.1粗酶液的提取流程" |
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