CN105749104A - Traditional Chinese medicine preparation capable of developing intelligence and preparation method thereof - Google Patents
Traditional Chinese medicine preparation capable of developing intelligence and preparation method thereof Download PDFInfo
- Publication number
- CN105749104A CN105749104A CN201610205518.8A CN201610205518A CN105749104A CN 105749104 A CN105749104 A CN 105749104A CN 201610205518 A CN201610205518 A CN 201610205518A CN 105749104 A CN105749104 A CN 105749104A
- Authority
- CN
- China
- Prior art keywords
- parts
- group
- chinese medicine
- alpiniae oxyphyllae
- fructus alpiniae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9062—Alpinia, e.g. red ginger or galangal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/638—Ligustrum, e.g. Chinese privet
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Medical Informatics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a traditional Chinese medicine preparation capable of developing intelligence and a preparation method thereof.The traditional Chinese medicine preparation is prepared from, by weight, 500-1,000 parts of fructus alpiniae oxyphyllae, 300-600 parts of prepared tubers of multiflower knotweed, 300-600 parts of roots of hairy asiabell, 300-600 parts of radix pseudostellariae, 300-600 parts of glossy privet fruit, 300-600 parts of seeds of Chinese dodder, 150-300 parts of hericium erinaceus and 150-300 parts of shiitake mushrooms.The traditional Chinese medicine preparation capable of developing the intelligence is good in effect on treating hypomnesis and dysmnesia.According to the preparation method of the traditional Chinese medicine preparation capable of developing the intelligence, monarch drugs, minister drugs, adjuvant drugs and messenger drugs serve as different groups respectively and extracted with different masses of ethyl alcohol at different temperatures, the obtained traditional Chinese medicine preparation capable of developing the intelligence is higher in raw material use ratio, and the drug therapeutic effect is better.
Description
Technical field
The present invention relates to field of traditional Chinese.It is more particularly related to a kind of Fructus Alpiniae Oxyphyllae Chinese medicine preparation and preparation method thereof.
Background technology
Namely hypomnesis is easily forgotten, and dysmnesia refer to that individual is in and a kind of can not remember or the state of memory information or technical ability, and the material of memorize is forgotten the fastest recently, develops into remote amnesia gradually.Doctor trained in Western medicine thinks that hypomnesis is mainly become by neurasthenia, brain structural disease, study and work life tension pressure causes brain fag etc. to cause.Dementia, cerebral trauma, amnestic syndrome, developmental lag, poisoning etc. all stress can cause dysmnesia.Dysmnesia correlative factor includes acute or chronic anoxia, cardiac output reduction, water or electrolyte imbalance, nerve problems, too much environmental disturbances etc..The traditional Chinese medical science thinks brain being the marrow sea, the raw marrow of essence, kidney storing essence, and lower for kidney, upper for brain, void is then all empty." doctor rock volume four " says that kidney essense is filled and contains then brains and fill, and deficiency of kidney-essence is deficiency of marrow-reservoir then.Brains are filled, then normal hearing and eyesight, energetic；Brains are hollow, may occur in which hypomnesis.Kidney tonifying, essence replenishing benefit marrow is the important method alleviating hypomnesis.And blood has nutrition and the physiological function of moist whole body, blood follows row in arteries and veins, interior to internal organs, reaches outward skin and flesh muscles and bones, and such as ring for no reason, operation is not stopped, and each internal organs organ of whole body constantly plays nutrition and moistens effect." difficult difficult through 22 " says blood nourishing.Blood is mind activity material base, and blood is sufficient, and ability mind is clear, hale and hearty." the exhausted paddy of Ling Shu Miraculous Pivot or Divine Axis normal person " says blood vessels and profit, and spirit is occupy.If blood deficiency, without being supported, often there is palpitation with fear, insomnia, dreaminess, the disease such as forgetful in god.It is very helpful for enriching blood for promoting memory.And there is mutual transforming relationship, blood energy spermatogenesis, the important substance of kidney essense being hemopoietic liquid again between essence and blood.In view of to essence, marrow, blood research, the traditional Chinese medical science forms following memory theory: deficiency of kidney essence is said: suffer from a deficiency of the kidney be difficult give birth to marrow fill brain and cause heart and brain apraxia, cause ability of learning and memory to decline；Phlegm retention congestion is said: suffers from a deficiency of the kidney and can not evaporate that water is wet, warm promotion QI-blood circulation, causes organism metabolism obstacle, cause the pathological product accumulations such as turbid, the blood stasis of expectorant, hypomnesis.
Fructus Alpiniae Oxyphyllae is the dry mature fruit of Zingiberaceae Alpinia plants Fructus Alpiniae Oxyphyllae, warm in nature, and acrid in the mouth enters taste warp, has warming spleen and stopping diarrha, takes the photograph saliva, warming the kidney, effect of controlling nocturnal emission with astringent drugs reducing urination, for spleen-cold syndrome diarrhea, and cold type of pain in abdomen, the many salivas of mouth, enuresis due to deficiency of the kidney, frequent micturition, nebulousurine of passing out semen.
Radix Polygoni Multiflori Preparata is the dried root goods of polygonum multiflorum thunb, bitter in the mouth, sweet, puckery, slightly warm in nature, return liver, the heart, kidney channel, there is invigorating the liver and kidney, benefiting essence-blood, bone and muscle strengthening, crow beard and hair, change effect of turbid blood fat reducing, for blood deficiency and yellow complexion, vertigo and tinnitus, early whitening of beard and hair, soreness of the waist and knees, seminal emission, numb limbs and tense tendons, bleeding not during menses, hyperlipemia etc..
Radix Codonopsis derives from the dry root of Campanulaceae Radix Codonopsis, element flower Radix Codonopsis and radix codonpsis tangshen etc., and property is sweet, and taste is put down, and QI invigorating is promoted the production of body fluid, quenched the thirst.For treating the diseases such as weakness of the spleen and stomach, deficiency of QI in middle-JIAO, gas virtual loss, consumption of body fluid caused by febrile disease.
Radix Pseudostellariae is the dried root of pinkwort Radix Ginseng, sweet in the mouth, micro-hardship, flat, returns spleen lung meridian, replenishing QI to invigorate the spleen, promoting the production of body fluid for nourishing the lung, inappetence, after being ill weakness tired for insufficiency of the spleen body, deficiency of both vital energy and Yin, thirsty, the dryness of the lung dry cough of spontaneous perspiration etc..
Fructus Ligustri Lucidi is the dry mature fruit of Oleaceae Ligustrum plant Fructus Ligustri Lucidi, and sweet-bitter flavor is cool in nature, returns Liver and kidney warp, invigorating the liver and kidney, strong waist knee joint, cures mainly yin asthenia generating intrinsic heat, dizzy, order flower, tinnitus, soreness of the waist and knees, early whitening of beard and hair etc..
Semen Cuscutae is the dry mature seed of convolvulus cuscuta plant, abnormal smells from the patient is pungent, sweet, flat, warm, return liver,kidney,spleen warp, the kidney invigorating and essence nourishing, nourishing the liver to improve visual acuity, aches for the waist knee joint muscles and bones of deficiency of the liver and kindey, legs and feet weakness, impotence and seminal emission, crazy talk, frequent micturition, dripping urination, dizziness and blurred vision, blurring of vision, Hiccough and deaf and the disease such as women's leukorrhagia, habitual abortion.
Hericium erinaceus (Bull. Ex Fr.) Pers. is the sporophore of hedgehog fungus section fungus Hericium erinaceus (Bull. Ex Fr.) Pers., and property is flat, sweet in the mouth, and profit the five internal organs are aid digestion, and stomach invigorating, qi-restoratives, anticancer, kidney tonifying is smart, cures mainly the diseases such as anorexia and loose stool, gastric and duodenal ulcers, superficial gastritis, esophageal carcinoma, gastric cancer, dizziness, sexual impotence.
Lentinus Edodes is the edible medicinal fungi of holding concurrently of Basidiomycetes Agaricales Tricholomataceae Lentinus, and sweet in the mouth, property are flat, nontoxic, QI invigorating, not hungry, control wind removing blood stasis.
Along with worldwide competition pressure, cerebrovascular disease and aging trend, hypomnesis and amnemonic crowd's number are in rising trend, hypomnesis and dysmnesia cause learning capacity and ability of thinking etc. to decline, harm people's life and health, serious hypomnesis and dysmnesia are often maintained close ties with alzheimer disease and vascular dementia.Exploitation effectively preventing and treating hypomnesis and dysmnesia medicine are one of current medical research field vital tasks.Treatment at present prevents and treats hypomnesis and dysmnesia medicine is mainly one pack system or single component preparation is main, as: chemosynthesis medea, animality hormone, bathypelagic fish oil, Radix Ginseng extract, Cornu Cervi Pantotrichum, Semen Ginkgo extrac etc., but action pathway is single, wholistic therapy effect is still undesirable, some Western medicine produce dependency, and then increase the weight of the state of an illness, some long-term takings are likely to have more untoward reaction and conscience kidney toxic and side effects, by comparison, herbal mixture has a clear superiority in strengthening curative effect and reducing toxic and side effects, but still lack herbal mixture research and application and preparation at present, more lack above-mentioned 8 taste Special Traditional Chinese Medicine ethnic drug compatibility researchs and application and preparation.
Summary of the invention
It is an object of the invention to solve at least the above, and the advantage that at least will be described later is provided.
It is a still further object of the present invention to provide a kind of Fructus Alpiniae Oxyphyllae Chinese medicine preparation, selected Fructus Alpiniae Oxyphyllae, Radix Polygoni Multiflori Preparata, Radix Codonopsis, Radix Pseudostellariae, Fructus Ligustri Lucidi, Semen Cuscutae, Hericium erinaceus (Bull. Ex Fr.) Pers. and Lentinus Edodes 8 taste drug matching prepare, for treating hypomnesis and dysmnesia, respond well.
The preparation method that it is a still further object of the present invention to provide a kind of Fructus Alpiniae Oxyphyllae Chinese medicine preparation, Fructus Alpiniae Oxyphyllae is monarch drug, Radix Polygoni Multiflori Preparata, Fructus Ligustri Lucidi, Semen Cuscutae are ministerial drug, Radix Codonopsis, Radix Pseudostellariae are adjuvant drug, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes are for making medicine, using monarch drug, ministerial drug, adjuvant drug and make medicine as different groups, adopt the ethanol of different quality to extract at different temperature, raw material utilization rate is higher, better drug curative effect.
In order to realize these purposes according to the present invention and further advantage, it is provided that a kind of Fructus Alpiniae Oxyphyllae Chinese medicine preparation, including the raw material of following weight portion:
Fructus Alpiniae Oxyphyllae 500～1000 parts, Radix Polygoni Multiflori Preparata 300～600 parts, Radix Codonopsis 300～600 parts, Radix Pseudostellariae 300～600 parts, Fructus Ligustri Lucidi 300～600 parts, Semen Cuscutae 300～600 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 150～300 parts and 150～300 parts of Lentinus Edodes.
Preferably, including the raw material of following weight portion:
Fructus Alpiniae Oxyphyllae 600～800 parts, Radix Polygoni Multiflori Preparata 400～500 parts, Radix Codonopsis 400～500 parts, Radix Pseudostellariae 400～500 parts, Fructus Ligustri Lucidi 400500 parts, Semen Cuscutae 400～500 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 200～250 parts and 200～250 parts of Lentinus Edodes.
Preferably, Fructus Alpiniae Oxyphyllae Chinese medicine preparation is tablet, capsule or oral liquid formulation.
The present invention can further be realized by the preparation method of Fructus Alpiniae Oxyphyllae Chinese medicine preparation, comprises the following steps:
1) after above-mentioned raw materials being pulverized, sieve, after adding ethanol extraction, obtain the first extracting solution；
2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate, take after described first filtering residue adds distilled water and extract, filter, obtain the second filtering residue and the second filtrate, by described first filtrate and described second filtrate mixing, after reclaiming ethanol, obtain mixed liquor, mixed liquor is concentrated, obtains thick paste.
3) described thick paste is added adjuvant post-treatment and obtain described Fructus Alpiniae Oxyphyllae Chinese medicine preparation.
Preferably, following steps are specifically included:
1) count by weight, after Fructus Alpiniae Oxyphyllae, Radix Polygoni Multiflori Preparata, Radix Codonopsis, Radix Pseudostellariae, Fructus Ligustri Lucidi, Semen Cuscutae, Hericium erinaceus (Bull. Ex Fr.) Pers. and Lentinus Edodes are pulverized, sieve, after adding the ethanol extraction that volume fraction is 95%, obtain the first extracting solution；
2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate, after adding the distilled water of 1.2～3 times amount in described first filtering residue, 2～5h is heated at 55～80 DEG C, filter, obtain the second filtering residue and the second filtrate, by described first filtrate and described second filtrate mixing, after reclaiming ethanol, obtain mixed liquor, after being concentrated by mixed liquor, obtain thick paste；
3), after adding adjuvant in described thick paste, described Fructus Alpiniae Oxyphyllae Chinese medicine preparation is namely obtained.
Preferably, step 2) in by mixed liquor concentrate method particularly includes:
Use mixed liquor described in the microwave heating of 30～40kw, adjustment pressure is 40～60kpa, when the temperature of heating extremely described mixed liquor is 40 DEG C, the power regulating microwave heating is 20～28kw, adjustment pressure is 15～35kpa, being then heated to the temperature of described mixed liquor when being 60 DEG C, the power regulating microwave heating is 10～18kw, obtains described thick paste after maintaining 10～15min.
Preferably, step 3) in obtain mixed liquor after, pineal shell activated carbon is added in described mixed liquor, the mass ratio of described pineal shell activated carbon and described mixed liquor is 1:200, after stirring 10～20min, carry out ultrafiltration, the ultrafilter membrane adopting aperture to be 0.01～0.03 μm, operation pressure is 0.15～0.25Mpa, first pass through one-level filter membrane, collect one-level trapped fluid, then operation pressure be under 0.3～0.4Mpa by described one-level trapped fluid by two grades of filter membranes, concentrate again after collecting second entrapment liquid, obtain thick paste.
Preferably, step 1) middle extraction method particularly includes:
Using the Fructus Alpiniae Oxyphyllae after sieving as first group, in first group, add the ethanol that volume fraction is 95% of 1 times of first group of quality, at 70～75 DEG C, heat 2h, obtain one group of extracting solution；Using the Radix Polygoni Multiflori Preparata after sieving, Fructus Ligustri Lucidi and Semen Cuscutae as second group, in second group, add the ethanol that volume fraction is 95% of 2 times of second group of quality, at 65～70 DEG C, heat 2.5h, obtain two groups of extracting solution；Using the Radix Codonopsis after sieving, Radix Pseudostellariae as the 3rd group, in the 3rd group, add the ethanol that volume fraction is 95% of 1.5 times of the 3rd group of quality, at 60～65 DEG C, heat 1.5h, obtain three groups of extracting solution；Using the Hericium erinaceus (Bull. Ex Fr.) Pers. after sieving, Lentinus Edodes as the 4th group, the ethanol that volume fraction is 95% of 1.5 times of the 4th group of quality is added in the 4th group, at 65～70 DEG C, heat 1.5h, obtain four groups of extracting solution, after being mixed by one group of extracting solution to four groups extracting solution, obtain described first extracting solution.
Clinical practice: be used for treating the hypomnesis caused by body void and dysmnesia, can also be used for preventing and treating retarded, childhood hyperkinetic syndrome, senile dementia, parkinson disease, cerebral trauma or apoplexy sequela, gatism, anemia, osteoporosis, rickets etc., it is also possible to keep fit in teenager and healthy personage's brain-strengthening.
Effective dose and using method: oral: tablet: one time 1～2, every day 1～3 time；Capsule: one time 1～2, every day 1～3 time；Oral liquid: a 30～60ml, every day 1～3 time.Or follow the doctor's advice.
Effect duration: 2 years.
Storage: be placed in dry place, seal.
The present invention at least includes following beneficial effect:
1, the present invention provides the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of a kind of new treatment hypomnesis and memory disorder.The present invention passes on traditional Chinese medicine theory, selected Fructus Alpiniae Oxyphyllae, Radix Polygoni Multiflori Preparata, Radix Codonopsis, Radix Pseudostellariae, Fructus Ligustri Lucidi, Semen Cuscutae, Hericium erinaceus (Bull. Ex Fr.) Pers. and Lentinus Edodes 8 taste Chinese medicine ethnic drug compatibility agent, forms characteristic, and wherein the kind warming the kidney controlling nocturnal emission with astringent drugs of Fructus Alpiniae Oxyphyllae, warming spleen and stopping diarrha, for we's monarch drug；Radix Polygoni Multiflori Preparata is apt to invigorating the liver and kidney, be full of essence and blood, and Fructus Ligustri Lucidi, Semen Cuscutae are apt to invigorating the liver and kidney, for we's ministerial drug；Radix Codonopsis, Radix Pseudostellariae are apt to qi invigorating and lung moistening, for adjuvant drug；Hericium erinaceus (Bull. Ex Fr.) Pers., the kind complement void of Lentinus Edodes, for making medicine；Fructus Alpiniae Oxyphyllae, Semen Cuscutae etc. also can circulation of qi promoting, invigorate blood circulation, blood stasis dispelling, reduce phlegm；Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes also can be in harmonious proportion the property of medicine, profit the five internal organs etc..After 8 taste Chinese medicine ethnic drugs make Chinese traditional compound medicine, there are short essence and blood brains and merit that benefit god nourishes heart, simultaneously supporing yang and yin nourishing combines, supporing yang and not impairment of YIN, yin nourishing and do not damage sun, meet high then slow it, meet and cold select using warming therapy, reach body equilibrium between yin and yang state, thus treating the hypomnesis caused by body void and dysmnesia.
2, the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention is intimately associated modern medicine study, refine Fructus Alpiniae Oxyphyllae, Radix Polygoni Multiflori Preparata, Radix Codonopsis, Radix Pseudostellariae, Fructus Ligustri Lucidi, Semen Cuscutae, elite in Hericium erinaceus (Bull. Ex Fr.) Pers. and Lentinus Edodes 8 taste Chinese medicine, containing Fructus Alpiniae Oxyphyllae terpenoid, taurine, yakuchinone, linolenic acid, protocatechuic acid, cupreol, tetrahydroxystilbene glucosides, specnuezhenside, Fructus Ligustri Lucidi polysaccharide, oleanolic acid, rhodioside, Semen Cuscutae flavonoid, polysaccharide from Cuscuta chinensis, lobetyolin, Radix Codonopsis polysaccharide, Pseudostellaria Polysaccharide, ursolic acid, acacetin, luteolin, Hericium Erinaceus Polysaccharide, lentinan, many effective ingredient such as aminoacid and trace element, constitute new complicated pluralism and efficient mechanism of action.This kind of mechanism of action can improve Cerebral cortex and hippocampus SOD, GSH-Px, CAT activity and reduce MDA content, the oxidant defense system damage caused by antioxidant radical and level of lipid is raised, strengthens cerebral tissue oxidation resistance, delay brain aging；Reduce acetylcholine esterase vigor, improve monoamine neurotransmitter norepinephrine (NE), dopamine (DA), 5-hydroxy tryptamine (5-HT) content, coordinate each mediator function of nervous system, regulate each mediator relative scale, improve the transmission of cerebral tissue information；Reduce the expression of inflammatory cytokines in cortex and hippocampal tissue; antalgic and sedative; alleviate the various inflammatory reactions around cerebral tissue and edema, alleviate intracranial pressure and raise, improve local circulation; promote to absorb hematoma; scavenging free radicals or resist its toxicity, protects intracellular mitochondrial, is reducing the expression simultaneously facilitating anti-apoptosis factor that apoptosis initiation factor is expressed; suppress ischemic cranial nerve cell apoptosis, alleviate stress time hippocampal neuron in Ca2+Concentration raises, MDA receptor NR2B subunit high expressed, the cranial nerve cell damage that glutamic acid gathers in a large number and exitotoxicity causes, and alleviates the neural cell injury that amyloid-beta (A β) mediates and ischemia causes, protects cranial nerve；Protein tau phosphorylation is neurofibrillary tangles Crack cause in senile dementia (AD) patient's brain, and this kind of mechanism can suppress neurocyte Protein tau phosphorylation；Thereby through all many-sided pharmacological actions, form new Mutiple Targets and work in coordination with efficient mechanism of action, produce to improve memory effect significantly, and have no adverse reaction and toxic and side effects.The Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention have beneficial god nourish heart, enriching yin and nourishing kidney, rich in nutrition, the effect such as phlegm reduction of blood circulation promoting, can be used for treating hypomnesis and dysmnesia, can also be used for preventing and treating retarded, childhood hyperkinetic syndrome, senile dementia, parkinson disease, cerebral trauma or apoplexy sequela, gatism, anemia, osteoporosis, rickets etc., it is also possible to keep fit in teenager and healthy personage's brain-strengthening.
3, the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention can also raise thymic weight; increase thymosin and hormonal substance secretion; promote immunocyte to produce, grow, accept antigenic stimulus and differentiation, maturation; booster immunization regulates and maintains self tolerance; enhancing immunity; also improve immunocyte and remove foreign body ability; remove some Chinese medicines harmful metabolite in human body in time; stop noxious products that brain darling renal function is caused damage; blood pressure lowering simultaneously, atherosclerosis, protect blood vessel wall; improve cardiopulmonary renal function and blood circulation, the strong bone hemopoietic of spleen invigorating etc..
4, in the preparation method of the present invention, Fructus Alpiniae Oxyphyllae is monarch drug, Radix Polygoni Multiflori Preparata, Fructus Ligustri Lucidi, Semen Cuscutae is ministerial drug, Radix Codonopsis, Radix Pseudostellariae is adjuvant drug, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes is for making medicine, by monarch drug, ministerial drug, adjuvant drug and make medicine respectively as different groups, the ethanol adopting different quality extracts at different temperature, make monarch drug, ministerial drug, adjuvant drug and make medicine mix again after extracting respectively, extract together than whole raw materials, the extraction of active component is more abundant, neither affect monarch drug, ministerial drug, adjuvant drug and make the respective property of medicine of medicine, raw material utilization rate is higher, can make again complicated pluralism that 8 kinds of medicines constitute and efficient mechanism of action can better play a role, make better efficacy after medicine, take effect faster.
5, the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention is pure Chinese medicinal preparation, make full use of China and Guangxi Special Traditional Chinese Medicine Ethnic crude drugs resources advantage, skimming the cream off milk, above-mentioned medical material is transformed into the multiple dosage form facilitating modern to carry, preserving, take in time, convenient society crowd takes.The Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention by nourishing the brain and improving intelligence and keep fit diseases prevention combine, reliable in quality, evident in efficacy, have no adverse reaction and toxic and side effects, natural green environmental protection, mild in medicine property and, safety is higher, and Social Agree is higher, applicable crowd is wider, demand is relatively big, becomes modern society and responds actively fierce survival and development competition, cardiovascular and cerebrovascular disease and aging etc. and improve the newly selected of quality of life, and economic benefit, social benefit prospect are considerable.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part is also by by being understood by those skilled in the art the research of the present invention and practice.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to description word.
The invention provides a kind of Fructus Alpiniae Oxyphyllae Chinese medicine preparation, including the raw material of following weight portion:
Fructus Alpiniae Oxyphyllae 500～1000 parts, Radix Polygoni Multiflori Preparata 300～600 parts, Radix Codonopsis 300～600 parts, Radix Pseudostellariae 300～600 parts, Fructus Ligustri Lucidi 300～600 parts, Semen Cuscutae 300～600 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 150～300 parts and 150～300 parts of Lentinus Edodes.
Fructus Alpiniae Oxyphyllae Chinese medicine preparation provided by the invention, it is preferred that include the raw material of following weight portion:
Fructus Alpiniae Oxyphyllae 600～800 parts, Radix Polygoni Multiflori Preparata 400～500 parts, Radix Codonopsis 400～500 parts, Radix Pseudostellariae 400～500 parts, Fructus Ligustri Lucidi 400～500 parts, Semen Cuscutae 400～500 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 200～250 parts and 200～250 parts of Lentinus Edodes.
The Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention, can adding adjuvant and be made as tablet, capsule or oral liquid formulation, adjuvant is preferably: diluent is one or more in polyvidone, octadecanol, hexadecanol, stearic acid, citric acid, microcrystalline Cellulose, starch, lactose, mannitol and sodium bicarbonate etc.；Disintegrating agent is one or more in carboxymethyl starch sodium, low substituted hydroxy-propyl methylcellulose, crospolyvinylpyrrolidone etc.；Lubricant is magnesium stearate, Pulvis Talci or micropowder silica gel；Film coating material is one or more in hydroxypropyl methyl cellulose, propenoic acid resin series, carboxymethyl cellulose and ethyl cellulose etc.；Film coating material is one or more in hydroxypropyl methyl cellulose, propenoic acid resin series, carboxymethyl cellulose and ethyl cellulose；Slow releasing preparation is one or more in dried cream powder, framework material, disintegrating agent, diluent, lubricant.
Embodiment 1
1) count by weight, after Fructus Alpiniae Oxyphyllae 500 parts, Radix Polygoni Multiflori Preparata 300 parts, Radix Codonopsis 300 parts, Radix Pseudostellariae 300 parts, Fructus Ligustri Lucidi 300 parts, Semen Cuscutae 300 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 150 parts and 150 parts of Lentinus Edodes are pulverized respectively, sieve, after adding the ethanol extraction that volume fraction is 75%, obtain the first extracting solution.
2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate, after taking the distilled water that described first filtering residue adds 2 times amount, 3h is heated at 60 DEG C, filter, obtain the second filtering residue and the second filtrate, by described first filtrate and described second filtrate mixing, after reclaiming ethanol, obtain mixed liquor, mixed liquor is concentrated, obtains thick paste.
3) in described thick paste, starch mixing is added, the mass ratio of starch consumption and thick paste is 1:1.5, with 95% ethanol or starch slurry for binding agent, make soft material, granulate with 14 orders, dry at 55 DEG C, control granule moisture content only 2%, add particle weight 2% carboxymethyl starch sodium, the magnesium stearate of the 0.5% of particle weight, mixing.Adjust the upper and lower stamping press of tablet machine, control sheet hardness and tablet weight variation scope, tabletted, film coating, film-coating material is one or more in hydroxypropyl methyl cellulose, propenoic acid resin series, carboxymethyl cellulose and ethyl cellulose etc., namely obtains Sugarless type Fructus Alpiniae Oxyphyllae Chinese medicine tablet through quality inspection is qualified.
Embodiment 2
1) count by weight, after Fructus Alpiniae Oxyphyllae 1000 parts, Radix Polygoni Multiflori Preparata 600 parts, Radix Codonopsis 600 parts, Radix Pseudostellariae 600 parts, Fructus Ligustri Lucidi 600 parts, Semen Cuscutae 600 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 300 parts and 300 parts of whole mixing of Lentinus Edodes, pulverize, sieve, after adding the ethanol extraction that volume fraction is 75%, obtain the first extracting solution.
2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate, after taking the distilled water that described first filtering residue adds 1 times amount, 1h is heated at 70 DEG C, filter, obtain the second filtering residue and the second filtrate, by described first filtrate and described second filtrate mixing, after reclaiming ethanol, obtain mixed liquor, mixed liquor is concentrated, obtains thick paste.
3) described thick paste is dried, get dry extract powder, add dried cream powder quality 0.5% diluent, diluent is polyvidone, octadecanol, hexadecanol, stearic acid, citric acid, microcrystalline Cellulose, starch, lactose, one or more in mannitol or sodium bicarbonate, the disintegrating agent of the 1% of dried cream powder quality, disintegrating agent is carboxymethyl starch sodium, low substituted hydroxy-propyl methylcellulose, one or more in crospolyvinylpyrrolidone, the lubricant of the 0.5% of dried cream powder quality, lubricant is magnesium stearate, Pulvis Talci or micropowder silica gel etc., it is mixed evenly, dry granulation, tabletting, sugar coating, qualified through quality inspection, obtain Fructus Alpiniae Oxyphyllae Chinese medicine tablet.
Embodiment 3
1) count by weight, by Fructus Alpiniae Oxyphyllae 800 parts, Radix Polygoni Multiflori Preparata 400 parts, Radix Codonopsis 400 parts, Radix Pseudostellariae 500 parts, Fructus Ligustri Lucidi 400 parts, Semen Cuscutae 500 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 200 parts and 200 parts of Lentinus Edodes, after pulverizing respectively, sieve, after adding the ethanol extraction that volume fraction is 75%, obtain the first extracting solution.
2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate, after taking the distilled water that described first filtering residue adds 1.5 times amount, 2h is heated at 65 DEG C, filter, obtain the second filtering residue and the second filtrate, by described first filtrate and described second filtrate mixing, after reclaiming ethanol, obtain mixed liquor, mixed liquor is concentrated, obtains thick paste.
3) described thick paste is dried the powder that gets dry extract, add the diluent such as appropriate amount of starch, select No. 1 hollow gelatine capsule of hard, filled by capsule filling machine, control the loading amount scope at 0.2～0.3g, qualified through quality inspection, namely obtain Fructus Alpiniae Oxyphyllae Chinese medicinal capsule.
Embodiment 4
1) count by weight, after Fructus Alpiniae Oxyphyllae 600 parts, Radix Polygoni Multiflori Preparata 400 parts, Radix Codonopsis 400 parts, Radix Pseudostellariae 400 parts, Fructus Ligustri Lucidi 400 parts, Semen Cuscutae 400 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 200 parts and 200 parts of Lentinus Edodes are pulverized, sieve, using the Fructus Alpiniae Oxyphyllae after sieving as first group, the ethanol that volume fraction is 95% of 1 times of first group of quality is added in first group, at 70 DEG C, heat 2h, obtain one group of extracting solution；Using the Radix Polygoni Multiflori Preparata after sieving, Fructus Ligustri Lucidi and Semen Cuscutae as second group, in second group, add the ethanol that volume fraction is 95% of 2 times of second group of quality, at 65 DEG C, heat 2.5h, obtain two groups of extracting solution；Using the Radix Codonopsis after sieving, Radix Pseudostellariae as the 3rd group, in the 3rd group, add the ethanol that volume fraction is 95% of 1.5 times of the 3rd group of quality, at 60 DEG C, heat 1.5h, obtain three groups of extracting solution；Using the Hericium erinaceus (Bull. Ex Fr.) Pers. after sieving, Lentinus Edodes as the 4th group, the ethanol that volume fraction is 95% of 1.5 times of the 4th group of quality is added in the 4th group, at 65 DEG C, heat 1.5h, obtain four groups of extracting solution, after being mixed by one group of extracting solution to four groups extracting solution, obtain described first extracting solution.
null2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate，After adding the distilled water of 1.2 times amount in described first filtering residue，2h is heated at 55 DEG C，Filter，Obtain the second filtering residue and the second filtrate，By described first filtrate and described second filtrate mixing，After reclaiming ethanol，Obtain mixed liquor，Pineal shell activated carbon is added in described mixed liquor，The mass ratio of described pineal shell activated carbon and described mixed liquor is 1:200，After stirring 10min，Carry out ultrafiltration，The ultrafilter membrane adopting aperture to be 0.01 μm，Operation pressure is 0.15Mpa，First pass through one-level filter membrane，Collect one-level trapped fluid，Then operation pressure be under 0.3Mpa by described one-level trapped fluid by two grades of filter membranes，Collect second entrapment liquid to concentrate again as after mixed liquor，Method for concentration be use 30kw microwave heating described in mixed liquor，Adjustment pressure is 40kpa，When the temperature of heating extremely described mixed liquor is 40 DEG C，The power regulating microwave heating is 20kw，Adjustment pressure is 15kpa，It is then heated to the temperature of described mixed liquor when being 60 DEG C，The power regulating microwave heating is 10kw，Described thick paste is obtained after maintaining 10min.
3) being dried by described thick paste, get dry extract powder, is fully ground to becoming pastel with cyclodextrin 1:1 in mass ratio mixing by dried cream powder, cold drying, obtains cyclodextrin clathrate.Adopt hydroxypropyl methyl cellulose and acrylic resin as framework material, add polyvinylpyrrolidone and make binding agent, be mixed evenly to prepare soft material with cyclodextrin clathrate.By soft material cold drying, pulverize to obtain fine powder, then take fine powder addition lactose as diluent, granulate for wetting agent with 95% ethanol, cold drying, granulate, it is eventually adding magnesium stearate as lubricant, adjusts tablet weight and pressure, tabletting, qualified through quality inspection, namely obtain long-acting of Fructus Alpiniae Oxyphyllae traditional Chinese medicine slow-releasing.
Embodiment 5
1) count by weight, after Fructus Alpiniae Oxyphyllae 800 parts, Radix Polygoni Multiflori Preparata 500 parts, Radix Codonopsis 500 parts, Radix Pseudostellariae 500 parts, Fructus Ligustri Lucidi 500 parts, Semen Cuscutae 500 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 250 parts and 250 parts of Lentinus Edodes are pulverized, sieve, using the Fructus Alpiniae Oxyphyllae after sieving as first group, the ethanol that volume fraction is 95% of 800 parts is added in first group, at 75 DEG C, heat 2h, obtain one group of extracting solution；Using the Radix Polygoni Multiflori Preparata after sieving, Fructus Ligustri Lucidi and Semen Cuscutae as second group, in second group, add the ethanol that volume fraction is 95% of 2 times of second group of quality, at 70 DEG C, heat 2.5h, obtain two groups of extracting solution；Using the Radix Codonopsis after sieving, Radix Pseudostellariae as the 3rd group, in the 3rd group, add the ethanol that volume fraction is 95% of 1.5 times of the 3rd group of quality, at 65 DEG C, heat 1.5h, obtain three groups of extracting solution；Using the Hericium erinaceus (Bull. Ex Fr.) Pers. after sieving, Lentinus Edodes as the 4th group, the ethanol that volume fraction is 95% of 1.5 times of the 4th group of quality is added in the 4th group, at 70 DEG C, heat 1.5h, obtain four groups of extracting solution, after being mixed by one group of extracting solution to four groups extracting solution, obtain described first extracting solution.
null2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate，After adding the distilled water of 3 times amount in described first filtering residue，5h is heated at 80 DEG C，Filter，Obtain the second filtering residue and the second filtrate，By described first filtrate and described second filtrate mixing，After reclaiming ethanol，Obtain mixed liquor，Pineal shell activated carbon is added in described mixed liquor，The mass ratio of described pineal shell activated carbon and described mixed liquor is 1:200，After stirring 20min，Carry out ultrafiltration，The ultrafilter membrane adopting aperture to be 0.03 μm，Operation pressure is 0.25Mpa，First pass through one-level filter membrane，Collect one-level trapped fluid，Then operation pressure be under 0.4Mpa by described one-level trapped fluid by two grades of filter membranes，Collect second entrapment liquid to concentrate again as after mixed liquor，Method for concentration be use 40kw microwave heating described in mixed liquor，Adjustment pressure is 60kpa，When the temperature of heating extremely described mixed liquor is 40 DEG C，The power regulating microwave heating is 28kw，Adjustment pressure is 35kpa，It is then heated to the temperature of described mixed liquor when being 60 DEG C，The power regulating microwave heating is 18kw，Described thick paste is obtained after maintaining 15min.
3) adding medicinal correctives, preservative, suspending agent or solubilizing agent etc. in described thick paste, mixing, the adjustment total amount that adds water, to 3000ml, shakes up, fill, qualified namely obtains Fructus Alpiniae Oxyphyllae Chinese medicine oral liquid through checking.Wherein, the consumption of correctives is 1～4g/1000ml protein sugar, the consumption of preservative is 1～2g/1000ml sodium benzoate, and suspending agent is one or more in glycerol, syrup, sorbitol, arabic gum, tragakanta starch slurry, methylcellulose, sodium carboxymethyl cellulose and aluminum monostearate.Solubilizing agent adopts one or more in TWEEN Series, propylene glycol, cyclodextrin inclusion compound body, cholic acid and nucleic acid.
Embodiment 6
1) count by weight, after Fructus Alpiniae Oxyphyllae 700 parts, Radix Polygoni Multiflori Preparata 450 parts, Radix Codonopsis 450 parts, Radix Pseudostellariae 450 parts, Fructus Ligustri Lucidi 450 parts, Semen Cuscutae 450 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 220 parts and 220 parts of Lentinus Edodes are pulverized, sieve, using the Fructus Alpiniae Oxyphyllae after sieving as first group, the ethanol that volume fraction is 95% of 700 parts is added in first group, at 72 DEG C, heat 2h, obtain one group of extracting solution；Using the Radix Polygoni Multiflori Preparata after sieving, Fructus Ligustri Lucidi and Semen Cuscutae as second group, in second group, add the ethanol that volume fraction is 95% of 2 times of second group of quality, at 68 DEG C, heat 2.5h, obtain two groups of extracting solution；Using the Radix Codonopsis after sieving, Radix Pseudostellariae as the 3rd group, in the 3rd group, add the ethanol that volume fraction is 95% of 1.5 times of the 3rd group of quality, at 62 DEG C, heat 1.5h, obtain three groups of extracting solution；Using the Hericium erinaceus (Bull. Ex Fr.) Pers. after sieving, Lentinus Edodes as the 4th group, the ethanol that volume fraction is 95% of 1.5 times of the 4th group of quality is added in the 4th group, at 62 DEG C, heat 1.5h, obtain four groups of extracting solution, after being mixed by one group of extracting solution to four groups extracting solution, obtain described first extracting solution.
null2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate，After adding the distilled water of 2 times amount in described first filtering residue，3h is heated at 65 DEG C，Filter，Obtain the second filtering residue and the second filtrate，By described first filtrate and described second filtrate mixing，After reclaiming ethanol，Obtain mixed liquor，Pineal shell activated carbon is added in described mixed liquor，The mass ratio of described pineal shell activated carbon and described mixed liquor is 1:200，After stirring 15min，Carry out ultrafiltration，The ultrafilter membrane adopting aperture to be 0.02 μm，Operation pressure is 0.20Mpa，First pass through one-level filter membrane，Collect one-level trapped fluid，Then operation pressure be under 0.35Mpa by described one-level trapped fluid by two grades of filter membranes，Collect second entrapment liquid to concentrate again as after mixed liquor，Method for concentration be use 35kw microwave heating described in mixed liquor，Adjustment pressure is 50kpa，When the temperature of heating extremely described mixed liquor is 40 DEG C，The power regulating microwave heating is 25kw，Adjustment pressure is 30kpa，It is then heated to the temperature of described mixed liquor when being 60 DEG C，The power regulating microwave heating is 15kw，Described thick paste is obtained after maintaining 12min.
3) in described thick paste, add preservative, solubilizing agent etc., mixing, shake up after adding water, fill, qualified namely obtain Fructus Alpiniae Oxyphyllae Chinese medicine oral liquid through checking.
Test portion
1) acetylcholine esterase active inhibition test
Acetylcholine (Acetylcholine) is as the neurotransmitter in teleneuron secretion and the stimulation to muscle transmission, once terminate the transmission stimulated, it will resolved into choline and acetate by acetylcholinesterase.The symptoms such as the cognitive dysfunction that the acetyl cholinergic neuronal damage originating from brain basilar part can cause memory loss and study idea to reduce.The mensuration that acetylcholine esterase active suppresses adopts methods such as liking Germania High frequency leep electrode sterilization method to carry out deforming to use.
Acetylcholinesterase makes the thiocholine and 5 of the product generated during acetylcholine hydrolyzation, 5 ' dithio double; two (2-nitrobenzoic acid) react and generate the o-Carboxynitrobenzene of yellow, the o-Carboxynitrobenzene now generated is measured absorbance under 412nm, thus observes acetylcholine esterase active and suppress.Using the test tube of 500 μ l as reactor, add the 0.1M sodium phosphate buffer of 445 μ l and the test solution of various dose, put into the 18 μ l as coupling agent 0.001M 5,5 ' dithio double; two (2-nitrobenzoic acid) and as, after the 0.0075M Acetyl choline Iodide of 6 μ l of substrate, reacting 4 minutes at the temperature chambers of 25 DEG C.Start to react the acetylcholinesterase as enzyme at once adding 6 μ l and after the temperature chamber of 25 DEG C reacts 12 minutes, under 412nm, measure absorbance.Repeat to implement 3 all test group, try to achieve the meansigma methods of absorbance.The mixed liquor of embodiments of the invention 3 preparation is as test solution, various dose is adopted to measure absorbance respectively, putting into the 0.1M sodium phosphate buffer with mixed liquor Isodose as test solution thus recording the absorbance of each matched group every time, being calculated the rate of being inhibited.Computing formula is:
Suppression ratio (%)=[1-(absorbance of the absorbance/test group of matched group)] × 100%.
Test solution in another test group A, in its preparation method except not using raw material Fructus Alpiniae Oxyphyllae, all the other are all identical with the method preparing mixed liquor of embodiment 3, obtain mixed liquor A as test solution.Test solution in experiment group B, in its preparation method except not using raw material Radix Polygoni Multiflori Preparata, all the other are all identical with the method preparing mixed liquor of embodiment 3, obtain mixed liquid B as test solution.Meanwhile, positive controls employs the tacrine of 100 μ l/ml concentration, measures the acetylcholine esterase active for refining from electric eel and suppresses result, and result is as shown in table 1.
The mensuration that acetylcholine esterase active is suppressed by table 1
The tacrine of the mixed liquor of various dose with the 100 μ l/ml concentration used as positive controls, for object, is compared by the mixed liquor prepared with embodiments of the invention 3, then the high dose group of 150 μ l/ml presents the inhibiting activity of acetylcholinesterase of the best.Test group A, experiment group B compares with high dose group and can be seen that, the inhibiting activity of acetylcholinesterase of high dose group is substantially better than test group A and experiment group B, and the mixed liquor that embodiments of the invention 3 prepare is by Fructus Alpiniae Oxyphyllae, Radix Polygoni Multiflori Preparata, Radix Codonopsis, Radix Pseudostellariae, Fructus Ligustri Lucidi, Semen Cuscutae, the extracting solution of Hericium erinaceus (Bull. Ex Fr.) Pers. and Lentinus Edodes 8 taste Chinese medicine coordinates prepared, the certain main component Fructus Alpiniae Oxyphyllae therein respectively of test group A and experiment group B and important component Radix Polygoni Multiflori Preparata, therefore, in sum, by Fructus Alpiniae Oxyphyllae, Radix Polygoni Multiflori Preparata, Radix Codonopsis, Radix Pseudostellariae, Fructus Ligustri Lucidi, Semen Cuscutae, the extracting solution that Hericium erinaceus (Bull. Ex Fr.) Pers. and Lentinus Edodes 8 taste drug matching prepare presents significant acetylcholine esterase active inhibition, the transmission of information in cerebral tissue can be improved, thus improving memory.
2) poisonous side effect of medicine test
Healthy Kunming mouse is equally divided into 4 groups, one of which is as a control group, every day gives chow diet, its excess-three group administration group gives the feedstuff of the Fructus Alpiniae Oxyphyllae Chinese medicinal capsule prepared containing the embodiments of the invention 3 of 0.5%, 1% and 2% every day respectively, dosage respectively 0.5,1 and 2g/kgBW, each group of mice physiology sign, behavior and biochemical indicator is measured after 30 days, result shows that matched group is substantially the same with administration group mice physiology sign, behavior and biochemical indicator, show that the Fructus Alpiniae Oxyphyllae Chinese medicine tablet of the present invention physiological activity on mice is without impact, has no side effect.
3) impact on memory
3.1 step down tests
3.1.1 sample: the Fructus Alpiniae Oxyphyllae Chinese medicinal capsule net content of embodiments of the invention 3 preparation is 0.3g/ grain.Room temperature preservation, is for experiment.
3.1.2 experimental animal: SPF level Kunming mouse, body weight 18-22g, female, 240, animal credit number: SYXK (Hubei Province) 2003-0014, test temperature: 22-24 DEG C, humidity 65-80%.
3.1.3 dosage choice: human body recommended amounts every day is 1.5g/30kgBW, sets basic, normal, high three dosage with these 10,20,30 times, and respectively 0.5,1.0 and l.5g/kgBW.By the volume gavage mice of 0.1ml/10gBW, being made into respective concentration, matched group gavage distilled water with distilled water, gavage capacity is identical with animal subject group, continuous gavage 30 days.
3.1.4 test method: body weight 18～22g Kunming mouse, is randomly divided into 0.5,1.0 and l.5g/kgBW three dosage groups and a blank group, and each dosage treated animal is often group 10.Continuous gavage carried out diving tower training after 30 days.Animal being put into reaction chamber endoadaptation 3 minutes, pass to 36 volts of alternating currents immediately, the number of times (errors number of animal) that in recording 3 minutes, every Mus is shocked by electricity, in this, as school grade.Cyclical test after 24 hours, record first time jumps off incubation period and the 3 minutes interior errors number of platform.
3.1.5 result of the test
Table 2 is on the preclinical impact of step down test
The table 3 impact on step down test errors number
From table 2, the continuous gavage of Fructus Alpiniae Oxyphyllae Chinese medicinal capsule of the present invention is after 30 days, in step down test, when each dosage group of mice is trained, incubation period compares with matched group, there are no significant for difference (P > 0.05), testting incubation period after 24 hours, each dosage group is all longer than matched group incubation period.Wherein, middle and high dosage group compares with matched group, and difference has significance (P < 0.05, P < 0.05).
From table 3, the continuous gavage of Fructus Alpiniae Oxyphyllae Chinese medicinal capsule of the present invention is after 30 days, and in step down test, when each dosage group of mice is trained, average error number of times compares with matched group, and there are no significant for difference (P > 0.05).Testting average error number of times after 24 hours, each dosage group is respectively less than matched group, and wherein, high dose group compares with matched group, and difference has significance (P < 0.05).
In step down test, each dosage group all can significantly extend the incubation period that mice was test after 24 hours, compares with matched group, and difference has significance.Testting average error number of times after 24 hours, each dosage group is respectively less than matched group, and wherein, high dose group compares with matched group, and difference has significance.Above result of the test shows that SPF level Kunming mouse is had significantly improving and improves the effect of memory by the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention.
3.2 clinical trials
3.2.1 material and method
Sample: the Fructus Alpiniae Oxyphyllae Chinese medicinal capsule of embodiments of the invention 3 preparation and placebo.Two kinds of sample packagings, profile and the basic zero difference of character.
Test material: record has the instruction and the tape of stimulus words, picture material (point first, second two is overlapped), recorder, stopwatch, record paper that point to memory and learning by association.
Subjects: select 15～18 years old age student 100 people as subjects.Subjects schooling is basically identical,
Do not accept similar test, do not take the medicine relevant with this product function or health food in a short time.
Test packet and usage and dosage: this test adopts double-blind method, at random experimenter is divided into 2 groups, get rid of midway and stop experimenter, actual tests group 49 example, matched group 46 example for some reason.Test group takes Fructus Alpiniae Oxyphyllae Chinese medicinal capsule capsule, and matched group takes placebo, every day 2 times, each 2.50 days observing times.
3.2.2 observation index
A) record subjects includes parameter and the situations such as spirit, sleep, diet, defecation, blood pressure, physical signs.
B) unified use Chinese Academy of Sciences's psychology Clinical Memory Test.Scale score is looked into original point of each subtest after test.Efficacy measures includes pointing to memory, learning by association, image is freed recall, random shape re-recognizes and the contact of portrait feature is recalled.With this test data for measurement data, two groups of each subtest scale score t inspections are analyzed.Own control is adopted paired t-test.Parallel between group comparing the t inspection adopting two sample averages, the latter need to carry out homogeneity test of variance, and the data of nonnormal distribution or heterogeneity of variance carry out suitable variable conversion, wait meet normal state or variance neat after, carry out t inspection by the data of conversion；If conversion data still can not meet normal state or the neat requirement of variance, use t instead2Inspection or rank test.
3.2.3 result of the test
Table 4 is each group memory scale score before testing
Each group memory scale score after table 5 test
By table 4 and table 5 it can be seen that the sensing memory of test group, learning by association, image are freed recall, random shape is re-recognized and the contact of portrait feature is recalled to compare before and after several index Tests and had significant difference (P value is respectively less than 0.01)；Test subsequently points to memory, learning by association, image is freed recall, random shape re-recognizes and portrait feature contact memory compares with matched group, and except freeing recall except image, other indexs all have the difference (P value is respectively less than 0.01) of highly significant.
Meanwhile, each physical signs such as spirit in subjects observing time, sleep, diet, defecation are substantially unchanged, blood pressure are without significant change, and namely safety indexes does not all produce ANOMALOUS VARIATIONS, does not observe untoward reaction.
Result shows that the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention has significantly improving and improves the function of memory, and safety is good.
4) impact on immunity
4.1 instruments and material
Instrument: dual-beam ultraviolet spectrophotometer: UV-2100, Beijing Rayleigh Analytical Instrument Co., Ltd；Electronic balance: AE200, prunus mume (sieb.) sieb.et zucc. holder instrument Shanghai company；Centrifuge etc..
Fructus Alpiniae Oxyphyllae Chinese medicine tablet: the Fructus Alpiniae Oxyphyllae Chinese medicinal capsule of embodiments of the invention 3 preparation；Prednisone acetate tablets: Guangdong Huanan Pharmaceutical Co., Ltd.
Animal: SPF level Kun Ming mice, male and female dual-purpose, body weight 18～22g.Animal productiong credit number: osmanthus SCXK2009-0002, animal occupancy permit number: osmanthus SCXK2009-0004.
The impact on hypoimmunity mice immune organ quality of the 4.2 Fructus Alpiniae Oxyphyllae Chinese medicine tablets
Method: take mice 50, it is randomly divided into 5 groups: blank group, model group, high, medium and low dosage group (0.5,1.0,2.0g/kg/d), except blank group, other organizes mice gavage 40mg/kg/d in morning prednisolone acetate, blank group isometric(al) water, afternoon high, medium and low dosage group mouse stomach relative medicine, blank group and model group gavage isometric(al) water, continuous 12d.After last gastric infusion 1h, put to death mice, blot residual blood with filter paper, claim weight, take spleen and thymus, claim quality with electronic balance, calculate Thymus and spleen index (I): I spleen=mSpleen/mBody；IThymus=mThymus/mBody。
4.3 results
The table 6 impact (X ± s, n=10) on hypoimmunity mice index and spleen index, thymus index
Note: compare with blank group, 1. P < 0.01: compare with model group, 2. P < 0.05,3. P < 0.01.
As can be seen from Table 6, comparing with blank group, model group mouse spleen index, thymus index all significantly reduce (P < 0.01), it was shown that prednisolone acetate causes immunodeficiency models success.Basic, normal, high dosage group all improves hypoimmunity mice index and spleen index, has significant (P < 0.01) with model group comparing difference.Basic, normal, high dosage group all improves hypoimmunity mice thymus index, has significant (P < 0.05) with model group comparing difference.Result of the test shows that SPF level Kunming mouse is had the effect significantly improving immunity by the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention.
5) other effects
The diversification mechanism of action that in the Fructus Alpiniae Oxyphyllae Chinese medicine preparation of the present invention, plurality of active ingredients is constituted can stop the accumulation of noxious products; reduce and brain darling renal function is caused damage; thus reducing blood pressure; atherosclerosis; protection blood vessel wall; improving cardiopulmonary renal function and blood circulation, bone hemopoietic is good in spleen invigorating, therefore can be used for teenager and healthy personage's brain-strengthening is kept fit.
Although embodiment of the present invention are disclosed as above, but listed utilization that it is not restricted in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, it is easily achieved other amendment, therefore, under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited to specific details and shown here as the embodiment with description.
Claims (8)
1. a Fructus Alpiniae Oxyphyllae Chinese medicine preparation, it is characterised in that include the raw material of following weight portion:
Fructus Alpiniae Oxyphyllae 500～1000 parts, Radix Polygoni Multiflori Preparata 300～600 parts, Radix Codonopsis 300～600 parts, Radix Pseudostellariae 300～600 parts, Fructus Ligustri Lucidi 300～600 parts, Semen Cuscutae 300～600 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 150～300 parts and 150～300 parts of Lentinus Edodes.
2. Fructus Alpiniae Oxyphyllae Chinese medicine preparation as claimed in claim 1, it is characterised in that include the raw material of following weight portion:
Fructus Alpiniae Oxyphyllae 600～800 parts, Radix Polygoni Multiflori Preparata 400～500 parts, Radix Codonopsis 400～500 parts, Radix Pseudostellariae 400～500 parts, Fructus Ligustri Lucidi 400～500 parts, Semen Cuscutae 400～500 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 200～250 parts and 200～250 parts of Lentinus Edodes.
3. Fructus Alpiniae Oxyphyllae Chinese medicine preparation as claimed in claim 1, it is characterised in that Fructus Alpiniae Oxyphyllae Chinese medicine preparation is tablet, capsule or oral liquid formulation.
4. the preparation method of a Fructus Alpiniae Oxyphyllae Chinese medicine preparation as claimed in claim 1, it is characterised in that comprise the following steps:
1) after above-mentioned raw materials being pulverized, sieve, after adding ethanol extraction, obtain the first extracting solution；
2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate, take after described first filtering residue adds distilled water and extract, filter, obtain the second filtering residue and the second filtrate, by described first filtrate and described second filtrate mixing, after reclaiming ethanol, obtain mixed liquor, mixed liquor is concentrated, obtains thick paste.
3) described thick paste is added adjuvant post-treatment and obtain described Fructus Alpiniae Oxyphyllae Chinese medicine preparation.
5. the preparation method of Fructus Alpiniae Oxyphyllae Chinese medicine preparation as claimed in claim 4, it is characterised in that specifically include following steps:
1) count by weight, after Fructus Alpiniae Oxyphyllae, Radix Polygoni Multiflori Preparata, Radix Codonopsis, Radix Pseudostellariae, Fructus Ligustri Lucidi, Semen Cuscutae, Hericium erinaceus (Bull. Ex Fr.) Pers. and Lentinus Edodes are pulverized, sieve, after adding the ethanol extraction that volume fraction is 95%, obtain the first extracting solution；
2) described first extracting solution is filtrated to get the first filtering residue and the first filtrate, after adding the distilled water of 1.2～3 times amount in described first filtering residue, 2～5h is heated at 55～80 DEG C, filter, obtain the second filtering residue and the second filtrate, by described first filtrate and described second filtrate mixing, after reclaiming ethanol, obtain mixed liquor, after being concentrated by mixed liquor, obtain thick paste；
3), after adding adjuvant in described thick paste, described Fructus Alpiniae Oxyphyllae Chinese medicine preparation is namely obtained.
6. the preparation method of Fructus Alpiniae Oxyphyllae Chinese medicine preparation as claimed in claim 4, it is characterised in that step 2) in mixed liquor is concentrated method particularly includes:
Use mixed liquor described in the microwave heating of 30～40kw, adjustment pressure is 40～60kpa, when the temperature of heating extremely described mixed liquor is 40 DEG C, the power regulating microwave heating is 20～28kw, adjustment pressure is 15～35kpa, being then heated to the temperature of described mixed liquor when being 60 DEG C, the power regulating microwave heating is 10～18kw, obtains described thick paste after maintaining 10～15min.
7. the preparation method of Fructus Alpiniae Oxyphyllae Chinese medicine preparation as claimed in claim 4, it is characterized in that, step 3) in obtain mixed liquor after, pineal shell activated carbon is added in described mixed liquor, the mass ratio of described pineal shell activated carbon and described mixed liquor is 1:200, after stirring 10～20min, carry out ultrafiltration, the ultrafilter membrane adopting aperture to be 0.01～0.03 μm, operation pressure is 0.15～0.25Mpa, first pass through one-level filter membrane, collect one-level trapped fluid, then operation pressure be under 0.3～0.4Mpa by described one-level trapped fluid by two grades of filter membranes, concentrate again after collecting second entrapment liquid, obtain thick paste.
8. the preparation method of Fructus Alpiniae Oxyphyllae Chinese medicine preparation as claimed in claim 4, it is characterised in that step 1) middle extraction method particularly includes:
Using the Fructus Alpiniae Oxyphyllae after sieving as first group, in first group, add the ethanol that volume fraction is 95% of 1 times of first group of quality, at 70～75 DEG C, heat 2h, obtain one group of extracting solution；Using the Radix Polygoni Multiflori Preparata after sieving, Fructus Ligustri Lucidi and Semen Cuscutae as second group, in second group, add the ethanol that volume fraction is 95% of 2 times of second group of quality, at 65～70 DEG C, heat 2.5h, obtain two groups of extracting solution；Using the Radix Codonopsis after sieving, Radix Pseudostellariae as the 3rd group, in the 3rd group, add the ethanol that volume fraction is 95% of 1.5 times of the 3rd group of quality, at 60～65 DEG C, heat 1.5h, obtain three groups of extracting solution；Using the Hericium erinaceus (Bull. Ex Fr.) Pers. after sieving, Lentinus Edodes as the 4th group, the ethanol that volume fraction is 95% of 1.5 times of the 4th group of quality is added in the 4th group, at 65～70 DEG C, heat 1.5h, obtain four groups of extracting solution, after being mixed by one group of extracting solution to four groups extracting solution, obtain described first extracting solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610205518.8A CN105749104A (en) | 2016-04-05 | 2016-04-05 | Traditional Chinese medicine preparation capable of developing intelligence and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610205518.8A CN105749104A (en) | 2016-04-05 | 2016-04-05 | Traditional Chinese medicine preparation capable of developing intelligence and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105749104A true CN105749104A (en) | 2016-07-13 |
Family
ID=56347225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610205518.8A Pending CN105749104A (en) | 2016-04-05 | 2016-04-05 | Traditional Chinese medicine preparation capable of developing intelligence and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105749104A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106310083A (en) * | 2016-09-27 | 2017-01-11 | 海南三风友制药有限公司 | Fructus alpiniae oxyphyllae urination decreasing oral liquid composition and preparation method thereof |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104583745A (en) * | 2012-06-20 | 2015-04-29 | M&B工程有限责任公司 | Machine and method for balancing the wheels of a vehicle |
-
2016
- 2016-04-05 CN CN201610205518.8A patent/CN105749104A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104583745A (en) * | 2012-06-20 | 2015-04-29 | M&B工程有限责任公司 | Machine and method for balancing the wheels of a vehicle |
Non-Patent Citations (1)
| Title |
|---|
| 夏翔等: "《一百天学中医内科》", 31 October 2015, 上海科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106310083A (en) * | 2016-09-27 | 2017-01-11 | 海南三风友制药有限公司 | Fructus alpiniae oxyphyllae urination decreasing oral liquid composition and preparation method thereof |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101601827B (en) | Traditional Chinese medicine for preventing and/or treating dry syndrome and slowing down aging and preparation method thereof | |
| CN107455625A (en) | A kind of nutrient for plants beverage and its preparation technology with antifatigue weight losing function | |
| CN114042126B (en) | Traditional Chinese medicine composition for ascending clear, eliminating turbid, promoting diuresis, benefiting qi, losing weight and reducing fat and preparation method thereof | |
| CN110664906B (en) | Preparation method of health food with blood sugar reducing function | |
| CN104225417A (en) | Traditional Chinese medicine composition with function of assisting improvement of memory and preparation method thereof | |
| CN105749104A (en) | Traditional Chinese medicine preparation capable of developing intelligence and preparation method thereof | |
| CN116509971A (en) | Traditional Chinese medicine formula, traditional Chinese medicine liquid and medicinal preparation with intelligence improving effect | |
| CN103735973A (en) | Traditional Chinese medicine composition for improving memory and preparation method thereof | |
| CN106266239A (en) | Improve compositions of memory and its preparation method and application | |
| CN114098085B (en) | Functional food composition with auxiliary memory improving function and application thereof | |
| CN105982905A (en) | Application of 20 (R)-ginsenoside Rg3 in preparing medicine for preventing and/or treating obesity and medicine | |
| CN108904697A (en) | A kind of female irregular menstruation conditioning cream and preparation method thereof | |
| CN104256618B (en) | A kind of hypoglycemic food, health products or pharmaceutical composition | |
| CN112089784A (en) | Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating diseases caused by atherosclerosis | |
| CN102028937B (en) | SOD (Super Oxide Dlsmutase) medicament composition for resisting ageing, reducing blood pressure and blood fat and preparation method thereof | |
| CN105194355A (en) | Traditional Chinese medicine preparation for treating primary hypertension | |
| CN106177476A (en) | A kind of Hyperglycemic health care compositions comprising Herba Dendrobii and Pericarpium Citri Reticulatae | |
| CN104547264A (en) | Capsules for improving memory and preparation method thereof | |
| CN106138795B (en) | Traditional Chinese medicine composition for treating premature beat and preparation method thereof | |
| CN112089783B (en) | Application of traditional Chinese medicine composition in preparation of medicine for preventing or/and treating obesity | |
| CN115886258B (en) | Composite probiotic composition for improving physiological memory attenuation | |
| CN108478701A (en) | A kind of lozenge of strengthen immunity | |
| CN105343508A (en) | Method for preparing traditional Chinese medicine composition for treating climacteric syndrome | |
| CN107136498B (en) | A kind of spot-removing beautifying face dew and preparation method | |
| CN105169259A (en) | Prescription for treating manic-depressive psychosis and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160713 |
|
| RJ01 | Rejection of invention patent application after publication |