CN105696314A - Preparation method of graphene/silk biofilm for promoting cell growth and detecting NO signals - Google Patents
Preparation method of graphene/silk biofilm for promoting cell growth and detecting NO signals Download PDFInfo
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- CN105696314A CN105696314A CN201610223457.8A CN201610223457A CN105696314A CN 105696314 A CN105696314 A CN 105696314A CN 201610223457 A CN201610223457 A CN 201610223457A CN 105696314 A CN105696314 A CN 105696314A
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
- D06M11/73—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof
- D06M11/74—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof with carbon or graphite; with carbides; with graphitic acids or their salts
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/322—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing nitrogen
- D06M13/402—Amides imides, sulfamic acids
- D06M13/432—Urea, thiourea or derivatives thereof, e.g. biurets; Urea-inclusion compounds; Dicyanamides; Carbodiimides; Guanidines, e.g. dicyandiamides
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/322—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing nitrogen
- D06M13/402—Amides imides, sulfamic acids
- D06M13/438—Sulfonamides ; Sulfamic acids
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
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- D—TEXTILES; PAPER
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- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/10—Animal fibres
Abstract
The invention relates to the field of biological application of graphene, in particular to a preparation method of graphene/silk biofilm for promoting cell growth and detecting NO signals. The preparation method includes steps of 1) adding sodium hydroxide and pyrenebutyric acid into graphene oxide suspension; 2) soaking silk cloth into the graphene oxide suspension, and drying after sufficiently contacting; 3) placing the silk cloth into hydradize hydrate to reduce; 4) taking the silk cloth out and repeatedly washing with deionized water and drying; 5) repeating the steps 2), 3) and 4) several times, to produce silk cloth wrapped by multilayer graphene; 6) placing the graphene-silk film into EDC and NHS for soaking; 7) dropping RGD peptide solution onto the surface of the graphene-silk film, and sufficiently reacting to produce the graphene/silk biofilm; 8) flushing the biolfilm by PBS buffer liquid to adjust pH to be neutral and drying.
Description
Technical field
The present invention relates to the preparation and application field of Graphene, be specially a kind of biomembranous preparation method of the graphene/silk for detecting NO。
Background technology
Graphene (Graphene) is the two dimensional crystal of the only one layer of atomic thickness being stripped out from graphite material, being made up of carbon atom, is thin, the hardest nano material in known world。Graphene all has the excellent properties of uniqueness in every respect, and has wide application space in multiple fields, is the study hotspot got most of the attention at present。Research for Graphene at present is concentrated mainly on the fields such as its physics, chemistry and materialogy, in addition, Graphene and derivant thereof are also very rapid in the development of biological field, especially graphene oxide has many new developments in biological and medical field application, the aspect such as such as targeted drug transport, biological detection。The composite performance prepared by Graphene is carried out functional modification is more superior, unique effect is played in more field, the Graphene biomembrane of functionalization here can play a role in biologic medical field medical treatment such as living cells detection and medication effect screenings, because cell can be grown on graphene-based functional biological film, the Graphene biomembrane of functionalization can also be good biosensor simultaneously。
Summary of the invention
It is an object of the invention to provide a kind of biomembranous preparation method of graphene/silk promoting Growth of Cells and NO signal can being detected, the method of preparation is easy, the graphene/silk prepared is soft, is expected to become following wearable product, possesses higher practical value。
The present invention promotes Growth of Cells and can detect the biomembranous preparation method of graphene/silk of NO signal, comprises the steps of
1) graphene oxide is dispersed in water, and ultrasonic obtains graphene oxide suspension, then add sodium hydroxide and pyrene butanoic acid, obtain mixed solution；
2) silkworm silk cloth is immersed in step 1) solution in about 20 ~ 30 minutes, make silkworm silk cloth and solution be fully contacted, then silkworm silk cloth taken out and put and dry in an oven；
3) the silkworm silk cloth of drying is put in hydrazine hydrate solution, keep reducing when 80 degrees Celsius；
4) the silkworm silk cloth after reduction is taken out, repeatedly rinse with deionized water, place in vacuum drying oven dry, obtain having wrapped up the silkworm silk cloth of Graphene after drying；
5) repeat the above steps 2), 3) and 4) several times, it is possible to obtain multi-layer graphene parcel silkworm silk cloth；
6) Graphene step 5) obtained-silkworm silk film is placed with in the mixed solution of 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide (EDC) and N-hydroxy-succinamide sulfonate sodium (NHS) and soaks；
7) again arginyl-glycyl-aspartic acid (RGD peptide) solution is dripped to step 6) the surface of Graphene-silkworm silk film, fully react, obtain graphene/silk biomembrane；
8) biomembrane PBS rinses adjustment pH value to dry after neutrality。
Further, described step 1) in graphene oxide be commercially available and by obtaining after graphite is aoxidized material；The concentration of graphene oxide suspension is 0.1 ~ 10mg/mL；In mixed solution, the concentration of sodium hydroxide is 4 ~ 40mg/mL, and pyrene butyric acid density is 5 ~ 50mg/mL。
Further, described step 3) in the concentration of hydrazine hydrate solution be 60 ~ 90wt%, the heated at constant temperature time is 1 ~ 3 hour。
Further, described step 4) in the temperature of drying baker be 40 ~ 80 degrees Celsius, drying time is 4 ~ 8 hours。
Further, described step 6) in EDC and NHS mixed solution in the molar concentration that molar concentration is 3-6mol/mL, NHS of EDC be 8-12mol/mL, soak time is 0.5 ~ 1 hour。
Further, described step 7) in the concentration of RGD peptide solution be 0.5 ~ 3mg/mL, the response time is 1 ~ 2 hour。
Accompanying drawing explanation
Fig. 1 is the graphene/silk biomembrane of a layer of the preparation of embodiment 1。
Fig. 2 is the graphene/silk biomembrane of the three layers of the preparation of embodiment 2。
Fig. 3 is the detection signal that the graphene/silk biomembrane of embodiment 2 utilizes cyclic voltammetry。Curve 1 and curve 2 respectively do not have NO and have the curve in NO situation。
Fig. 4 is the optical picture of the HaCat cell cultivated with 6 orifice plates。Fig. 4 (a) and (b) respectively do not have biomembrane and have biomembranous cell growth status。
Detailed description of the invention
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail。
Embodiment 1
The present invention promotes Growth of Cells and can detect the biomembranous preparation method of Graphene-silkworm silk of NO signal, comprises the steps of
1) graphene oxide being commercially available is dispersed in water, and the ultrasonic graphene oxide suspension obtaining 2.0mg/mL, then add sodium hydroxide and pyrene butanoic acid, obtain mixed solution, in mixed solution, the concentration of sodium hydroxide is 8.0mg/mL, and pyrene butyric acid density is 10mg/mL；
2) silkworm silk cloth is immersed in step 1) solution in about 30 minutes, make silkworm silk cloth and solution be fully contacted, then silkworm silk cloth taken out and put and dry in an oven；
3) the silkworm silk cloth of drying is put in hydrazine hydrate solution, keep reducing when 80 degrees Celsius；
4) the silkworm silk cloth after reduction is taken out, repeatedly rinse with deionized water, place in vacuum drying oven dry, obtain having wrapped up the silkworm silk cloth of a layer graphene after drying；
5) Graphene step 4) obtained-silkworm silk film is placed with in the mixed solution of 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide (EDC) and N-hydroxy-succinamide sulfonate sodium (NHS) and soaks；
6) again arginyl-glycyl-aspartic acid (RGD peptide) solution is dripped to step 5) the surface of Graphene-silkworm silk film, fully react, obtain graphene/silk biomembrane；
7) biomembrane PBS rinses adjustment pH value to dry after neutrality。
Fig. 1 is the graphene/silk biomembrane of a layer of the preparation of embodiment 1。
Embodiment 2
The present invention promotes Growth of Cells and can detect the biomembranous preparation method of Graphene-silkworm silk of NO signal, comprises the steps of
1) graphene oxide synthesized by Hummers method is dispersed in water, and ultrasonic obtain 2.4mg/mL graphene oxide suspension, then add sodium hydroxide and pyrene butanoic acid, obtain mixed solution, in mixed solution, the concentration of sodium hydroxide is 9.0mg/mL, and pyrene butyric acid density is 12mg/mL；
2) silkworm silk cloth is immersed in step 1) solution in about 30 minutes, make silkworm silk cloth and solution be fully contacted, then silkworm silk cloth taken out and put and dry in an oven；
3) the silkworm silk cloth of drying is put in hydrazine hydrate solution, keep reducing when 80 degrees Celsius；
4) the silkworm silk cloth after reduction is taken out, repeatedly rinse with deionized water, place in vacuum drying oven dry, obtain having wrapped up the silkworm silk cloth of Graphene after drying；
5) repeat the above steps 2), 3) and 4) three times, it is possible to obtain the silkworm silk cloth of three layer graphenes parcels；
6) Graphene step 5) obtained-silkworm silk film is placed with in the mixed solution of 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide (EDC) and N-hydroxy-succinamide sulfonate sodium (NHS) and soaks；
7) again arginyl-glycyl-aspartic acid (RGD peptide) solution is dripped to step 6) the surface of Graphene-silkworm silk film, fully react, obtain graphene/silk biomembrane；
8) biomembrane PBS rinses adjustment pH value to dry after neutrality。
Fig. 2 is the graphene/silk biomembrane of the three layers of the preparation of embodiment 2。
Fig. 3 is the detection signal that the graphene/silk biomembrane of embodiment 2 utilizes cyclic voltammetry。Curve 1 and curve 2 respectively do not have NO and have the curve in NO situation。It can be seen that biomembrane can be utilized to detect NO signal。
Fig. 4 is the optical picture of the HaCat cell cultivated with 6 orifice plates。Fig. 4 (a) and (b) respectively do not have biomembrane and have biomembranous cell growth status, it can be seen that have biomembranous existence, promoted the growth of cell。
What finally illustrate is, preferred embodiment above is only in order to illustrate technical scheme and unrestricted, although the present invention being described in detail by above-mentioned preferred embodiments, but skilled artisan would appreciate that, in the form and details it can be made various change, without departing from claims of the present invention limited range。
Claims (6)
1. promote Growth of Cells and the biomembranous preparation method of graphene/silk of NO signal can be detected, it is characterised in that comprising the steps of
1) graphene oxide is dispersed in water, and ultrasonic obtains graphene oxide suspension, then add sodium hydroxide and pyrene butanoic acid, obtain mixed solution；
2) silkworm silk cloth is immersed in step 1) solution in about 20 ~ 30 minutes, make silkworm silk cloth and solution be fully contacted, then silkworm silk cloth taken out and put and dry in an oven；
3) the silkworm silk cloth of drying is put in hydrazine hydrate solution, keep reducing when 80 degrees Celsius；
4) the silkworm silk cloth after reduction is taken out, repeatedly rinse with deionized water, place in vacuum drying oven dry, obtain having wrapped up the silkworm silk cloth of Graphene after drying；
5) repeat the above steps 2), 3) and 4) several times, it is possible to obtain multi-layer graphene parcel silkworm silk cloth；
6) Graphene-gauze sheet step 5) obtained is placed with in the mixed solution of 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide (EDC) and N-hydroxy-succinamide sulfonate sodium (NHS) and soaks；
Arginyl-glycyl-aspartic acid (RGD peptide) solution is dripped to step 6 by 7 again) the surface of Graphene-silkworm silk film, fully react, obtain graphene/silk biomembrane；
8) biomembrane PBS rinses adjustment pH value to dry after neutrality。
2. promotion the Growth of Cells according to claim 1 and biomembranous preparation method of graphene/silk of NO signal can be detected, it is characterized in that,, described step 1) in graphene oxide be commercially available and by obtaining after graphite is aoxidized material；The concentration of graphene oxide suspension is 0.1 ~ 10mg/mL；In mixed solution, the concentration of sodium hydroxide is 4 ~ 40mg/mL, and pyrene butyric acid density is 5 ~ 50mg/mL。
3. promotion the Growth of Cells according to claim 1 and biomembranous preparation method of graphene/silk of NO signal can be detected, it is characterised in that described step 3) in the concentration of hydrazine hydrate solution be 60 ~ 90wt%, the heated at constant temperature time is 1 ~ 3 hour。
4. promotion the Growth of Cells according to claim 1 and biomembranous preparation method of graphene/silk of NO signal can be detected, it is characterised in that described step 4) in the temperature of drying baker be 40 ~ 80 degrees Celsius, drying time is 4 ~ 8 hours。
5. promotion the Growth of Cells according to claim 1 and biomembranous preparation method of graphene/silk of NO signal can be detected, it is characterized in that, described step 7) in EDC and NHS mixed solution in the molar concentration of EDC be 3-6mol/mL, the molar concentration of NHS is 8-12mol/mL, and soak time is 0.5 ~ 1 hour。
6. promotion the Growth of Cells according to claim 1 and biomembranous preparation method of graphene/silk of NO signal can be detected, it is characterised in that described step 8) in the concentration of RGD peptide solution be 0.5 ~ 3mg/mL, the response time is 1 ~ 2 hour。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592936A (en) * | 2019-09-19 | 2019-12-20 | 西南大学 | Preparation of anti-ultraviolet silk fabric by using EDC/NHS solution |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181961A (en) * | 2011-03-07 | 2011-09-14 | 青岛大学 | Method for preparing graphene functionalized alginate fibers |
| US20140017322A1 (en) * | 2007-07-27 | 2014-01-16 | The Board Of Trustees Of The Leland Stanford Junior University | Supramolecular functionalization of graphitic nanoparticles for drug delivery |
| CN104328653A (en) * | 2014-10-11 | 2015-02-04 | 江南大学 | Method for multifunctional sorting of textile by using graphene oxide derivative |
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- 2016-04-12 CN CN201610223457.8A patent/CN105696314A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140017322A1 (en) * | 2007-07-27 | 2014-01-16 | The Board Of Trustees Of The Leland Stanford Junior University | Supramolecular functionalization of graphitic nanoparticles for drug delivery |
| CN102181961A (en) * | 2011-03-07 | 2011-09-14 | 青岛大学 | Method for preparing graphene functionalized alginate fibers |
| CN104328653A (en) * | 2014-10-11 | 2015-02-04 | 江南大学 | Method for multifunctional sorting of textile by using graphene oxide derivative |
Non-Patent Citations (2)
| Title |
|---|
| CHUN XIAN GUO ET AL.: "RGD-Peptide Functionalized Graphene Biomimetic Live-Cell Sensor for Real-Time Detection of Nitric Oxide Molecules", 《ACS NANO》 * |
| WENJING YUAN ET AL.: "Ultrasensitive and Selective Nitrogen Dioxide Sensor Based on SelfAssembled Graphene/Polymer Composite Nanofibers", 《ACS APPLIED MATERIALS & INTERFACES》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592936A (en) * | 2019-09-19 | 2019-12-20 | 西南大学 | Preparation of anti-ultraviolet silk fabric by using EDC/NHS solution |
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Application publication date: 20160622 |