CN102621208B - Preparation method and application of three-dimensional graphene electrode for electrochemical biosensor - Google Patents
Preparation method and application of three-dimensional graphene electrode for electrochemical biosensor Download PDFInfo
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- CN102621208B CN102621208B CN2012100753647A CN201210075364A CN102621208B CN 102621208 B CN102621208 B CN 102621208B CN 2012100753647 A CN2012100753647 A CN 2012100753647A CN 201210075364 A CN201210075364 A CN 201210075364A CN 102621208 B CN102621208 B CN 102621208B
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Abstract
The invention discloses a preparation method and application of a three-dimensional graphene electrode for an electrochemical biosensor. The preparation method comprises the following steps of: fixing spongy graphene in which industrially produced foam nickel is taken as a substrate and which has a three-dimensional structure and is synthesized through chemical vapor deposition on a glass or quartz sheet; connecting the spongy graphene with the three-dimensional structure and a wire by using a silver conductive adhesive; and coating organic silica gel on a connection point of the metal wire and the graphene for insulation to obtain a spongy graphene electrochemical electrode with the three-dimensional structure. The three-dimensional spongy graphene electrode has the outstanding characteristics of high conductivity, high specific surface area, high electrochemical stability and the like, is easily subjected to surface functional modification, and has high detection sensitivity to dopamine and nicotinamide adenine dinucleotide; and a highly sensitive electrochemical biosensor for non-enzymatically and selectively detecting glucose can be obtained after the surface of the electrode is modified by Co3O4.
Description
Technical field
The present invention relates to a kind of preparation method of the shape of the three-dimensional sponge for electrochemica biological sensing Graphene electrodes and in the application of electrochemica biological sensory field.
Background technology
Electrochemica biological sensor plays vital effect in current highly sensitive biomolecule detection, clinical medicine and genetic engineering research field.Wherein, the conductivity of electrochemical electrode, specific surface area and and with the compatibility of biomolecule be the key that affects electrochemica biological sensor detection sensitivity and detection limit, its performance is to the application of expansion electrochemica biological sensor and guarantee that its stable operation is most important.Yet traditional glass-carbon electrode exists poorly conductive, surface to be difficult to functional modification and the shortcoming such as specific surface area is low, make that electrochemica biological sensor is low to the detection sensitivity of biomolecule, specific selectivity is poor.Graphene is a kind of Novel Carbon Nanomaterials as Nobel prize for physics achievement in research appearance in 2010, it is the bi-dimensional cellular shape crystal structure by the monolayer carbon atomic building, electron mobility with superelevation, large specific surface area and excellent physics, chemistry, optics and mechanical property, the being on the scene effect transistor, the nanoelectronic bio-sensing, transparent conductive film, prepare composite has boundless application prospect, wherein the graphene modified glass-carbon electrode is studied widely in the bio-sensing field, the result of announcing as patent of invention CN 201110112989 shows that the glass-carbon electrode of graphene modified can detect rutin and Quercetin simultaneously, the film modified glass-carbon electrode of result proof Graphene/Nafion that patent of invention CN 200910084344.4 announces can be realized the Sensitive Detection of dopamine, but these method of modifying based on graphene oxide easily make Graphene reunite, be difficult to effectively to bring into play specific surface area that Graphene is large and the characteristic of high conductivity.
The spongy Graphene of three-dimensional structure has Stability Analysis of Structures, good conductivity, specific surface area and greatly, easily realizes the characteristics such as surface-functionalized modification, is the ideal electrode material for preparing the high sensitive electrochemical biology sensor.Yet, after not yet finding at present to utilize the spongy Graphene of three-dimensional structure directly to prepare electrochemical electrode functionalization, study report that it is applied at the electrochemica biological sensory field.
Summary of the invention
Technical matters:The purpose of this invention is to provide a kind of preparation method of the shape of the three-dimensional sponge for electrochemica biological sensing Graphene electrodes and in the application of electrochemica biological sensory field.
Technical scheme:A kind of method for preparing three-dimensional sponge shape Graphene electrochemical electrode provided by the present invention and in the application of electrochemica biological sensory field, the preparation method comprises following preparation process:
1) by chemical vapor deposition, the synthetic spongy graphene film of three-dimensional structure is fixed on glass sheet or piezoid surface with organic silica gel;
2) with conductive silver glue glue, three-dimensional sponge shape Graphene is connected with plain conductor;
3) tie point that organic silica gel is applied to plain conductor and Graphene is insulated, and forms NOT-function three-dimensional sponge shape Graphene electrodes after solidifying;
4) by CoCl
26H
2O, urea, H
2O adds in reactor, after stirring, puts into the glass sheet or the piezoid that are loaded with three-dimensional sponge shape graphene film, and 100-200 ℃ is reacted 5 ~ 20 hours.
5) the spongy graphene film that is loaded in glass sheet or piezoid surface after reacting, with after distilled water flushing 3-6 time, after drying in baking oven, is put into muffle furnace 300-500 ℃ of roasting 1-5 hour, formation functionalization three-dimensional sponge shape Graphene electrodes.
Described three-dimensional sponge shape graphene film is the square or rectangular that the diameter disk that is 0.1 ~ 5cm or the length of side are 0.1 ~ 5cm.
Described plain conductor material is copper or titanium or silver.
Described NOT-function three-dimensional sponge shape Graphene electrodes can be carried out the Electrochemical Detection of dopamine, nicotinamide adenine dinucleotide, uric acid.
Described functionalization three-dimensional sponge shape Graphene electrochemical electrode can carry out detecting without enzyme of glucose.
The selectivity that described functionalization three-dimensional sponge shape Graphene electrochemical electrode can carry out glucose, uric acid and ascorbic acid in blood plasma detects without enzyme.
Beneficial effect:Compare with existing electrochemical electrode biosensor technique, the invention has the advantages that
1, the present invention discloses a kind of novel three-dimensional sponge shape Graphene electrochemical electrode preparation method first, and this technology for preparing electrode is simple, cheap, be easy to realize the scale preparation;
2, the prepared three-dimensional sponge shape Graphene electrochemical electrode conductivity of the present invention is high, specific surface area is large, electrochemical stability good, detection sensitivity is high;
3, the prepared easy functional modification in three-dimensional sponge shape Graphene electrochemical electrode surface of the present invention, realize the highly sensitive and high specific selectivity of different biological molecules is detected.
The accompanying drawing explanation
Fig. 1. be the optical photograph of the three-dimensional sponge shape Graphene electrochemical electrode of preparation in embodiment 1.
Fig. 2. be the three-dimensional sponge shape Graphene electrochemical electrode of preparation in embodiment 1 and the comparison of traditional glass-carbon electrode cyclic voltammetry curve and impedance.
Fig. 3. the three-dimensional sponge shape Graphene electrochemical electrode prepared for the present invention in embodiment 2 is 5.6 o'clock detection limits to dopamine in signal to noise ratio (S/N ratio).
Fig. 4. for the spongy Graphene electrochemical electrode of the three-dimensional structure that in embodiment 3 prepared by the present invention detects the cyclic voltammetry curve of dopamine and uric acid simultaneously.
Fig. 5. be the Co that in embodiment 5 prepared by the present invention
3O
4The SEM photo of functionalization three-dimensional grapheme electrochemical electrode and to glucose without enzyme detection time-map of current.
Fig. 6. the three-dimensional sponge shape Graphene electrochemical electrode prepared for the present invention in embodiment 6 is through Co
3O
4The time m-map of current after modification, glucose in blood plasma, uric acid, ascorbic acid selectivity detected.
Embodiment
Below in conjunction with accompanying drawing and instantiation, the present invention is described in detail.
Embodiment 1:
The organic silica gel that the synthetic spongy graphene film of three-dimensional structure is 48g/L by 0.2mL concentration by chemical vapor deposition is fixed on glass sheet surface;
Conductive silver glue glue 0.2mg is connected three-dimensional grapheme under 20 ℃ with plain conductor one end, tack time is 5 minutes;
The tie point that the organic silica gel of 20mg is applied to plain conductor and Graphene is insulated, and be 5 hours set time, forms NOT-function three-dimensional sponge shape Graphene electrodes;
Three-dimensional sponge shape graphene film is the square that the length of side is 0.5cm.
The plain conductor material is titanium.
The optical photograph that Fig. 1 is the three-dimensional sponge shape Graphene electrodes for preparing of said method; Fig. 2 is the three-dimensional sponge shape Graphene electrodes for preparing of said method and the comparison of traditional glass-carbon electrode cyclic voltammetry curve and impedance.
Embodiment 2:
The organic silica gel that the synthetic spongy graphene film of three-dimensional structure is 48g/L by 0.2mL concentration by chemical vapor deposition is fixed on the piezoid surface;
Conductive silver glue glue 0.3mg is connected three-dimensional grapheme under 25 ℃ with plain conductor one end, tack time is 10 minutes;
The tie point that the organic silica gel of 35mg is applied to plain conductor and Graphene is insulated, and be 12 hours set time, forms NOT-function three-dimensional sponge shape Graphene electrodes.
The disk that the spongy graphene film of three-dimensional structure is diameter 2cm.
The plain conductor material is copper.
Fig. 3 is the time m-map of current that the above-mentioned NOT-function three-dimensional sponge shape Graphene utmost point carries out the dopamine detection.
Embodiment 3:
The organic silica gel that the synthetic spongy graphene film of three-dimensional structure is 48g/L by 0.2mL concentration by chemical vapor deposition is fixed on the piezoid surface;
Conductive silver glue glue 0.3mg is connected three-dimensional grapheme under 25 ℃ with plain conductor one end, tack time is 10 minutes;
The tie point that the organic silica gel of 50mg is applied to plain conductor and Graphene is insulated, and be 12 hours set time, forms NOT-function three-dimensional sponge shape Graphene electrodes.
The spongy graphene film of three-dimensional structure is the square that the length of side is 0.5cm.
The plain conductor material is silver.
Fig. 4 is the galvanochemistry cyclic voltammetry curve that the above-mentioned NOT-function three-dimensional sponge shape Graphene utmost point carries out dopamine and uric acid detection simultaneously.
Embodiment 4:
The organic silica gel that the synthetic spongy graphene film of three-dimensional structure is 48g/L by 0.2mL concentration by chemical vapor deposition is fixed on the piezoid surface;
Conductive silver glue glue 0.3mg is connected three-dimensional grapheme under 25 ℃ with plain conductor one end, tack time is 10 minutes;
The tie point that the organic silica gel of 50mg is applied to plain conductor and Graphene is insulated, and be 12 hours set time, forms NOT-function three-dimensional sponge shape Graphene electrodes.
The spongy graphene film of three-dimensional structure is the square that the length of side is 1cm.
The plain conductor material is titanium.
The above-mentioned NOT-function three-dimensional sponge shape Graphene utmost point can carry out the detection of nicotinamide adenine dinucleotide, and its detection limit reaches 50nM.
Embodiment 5:
The organic silica gel that the synthetic spongy graphene film of three-dimensional structure is 48g/L by 0.2mL concentration by chemical vapor deposition is fixed on glass sheet surface;
Conductive silver glue glue 0.4mg is connected three-dimensional grapheme under 25 ℃ with plain conductor one end, tack time is 20 minutes;
The tie point that the organic silica gel of 100mg is applied to plain conductor and Graphene is insulated, and be 24 hours set time, forms NOT-function three-dimensional sponge shape Graphene electrodes;
By 0.3g CoCl
26H
2O, 0.04g urea, 24gH
2O adds in the reactor of 0.05L, and glass bar stirred after 3 minutes, put into the glass sheet that is loaded with spongy graphene film, and 120 ℃ are reacted 10 hours.
The reacted glass sheet that is loaded with spongy graphene film, with after distilled water flushing 5 times, is dried 5 hours in 40 ℃ in baking oven, last, put into 400 ℃ of roastings of muffle furnace 2 hours, form functionalization three-dimensional sponge shape Graphene electrodes.
The square that the spongy graphene film of three-dimensional structure is length of side 1cm.
The plain conductor material is titanium.
The SEM picture that Fig. 5 is above-mentioned NOT-function three-dimensional sponge shape Graphene electrodes and carry out the time m-map of current that grape sugar detects without enzyme.
Embodiment 6:
The organic silica gel that the synthetic spongy graphene film of three-dimensional structure is 48g/L by 0.4mL concentration by chemical vapor deposition is fixed on glass sheet or piezoid surface;
Conductive silver glue glue 1mg is connected three-dimensional grapheme under 20 ℃ with plain conductor one end, tack time is 30 minutes;
The tie point that the organic silica gel of 120mg is applied to plain conductor and Graphene is insulated, and be 24 hours set time, forms NOT-function three-dimensional sponge shape Graphene electrodes;
By 0.24g CoCl
26H2O, 0.06g urea, 24gH
2O adds in the reactor of 0.05L, and glass bar stirred after 5 minutes, put into the glass sheet that is loaded with spongy graphene film, and 120 ℃ are reacted 16 hours.
The reacted glass sheet that is loaded with spongy graphene film or piezoid, with after distilled water flushing 5 times, are dried 4 hours in 50 ℃ in baking oven, last, put into 450 ℃ of roastings of muffle furnace 2 hours, form functionalization three-dimensional sponge shape Graphene electrodes.
The square that the spongy graphene film length of side of three-dimensional structure is 1cm.
The plain conductor material is silver.
Fig. 6 is that above-mentioned functions three-dimensional sponge shape Graphene electrodes is carried out the selectivity of glucose, uric acid and ascorbic acid in blood plasma without enzyme detection time-map of current.
Claims (6)
1. the preparation method of the three-dimensional grapheme electrode of an electrochemica biological sensing is characterized in that the method comprises the following steps:
1). the spongy graphene film of three-dimensional structure that chemical vapor deposition is synthetic is fixed on glass sheet or piezoid surface with organic silica gel;
2). with conductive silver glue glue, three-dimensional grapheme is connected with plain conductor one end;
3). the tie point that organic silica gel is applied to plain conductor and Graphene is insulated, and forms NOT-function three-dimensional sponge shape Graphene electrodes after solidifying;
4). by CoCl
26H
2O, urea and H
2O adds in reactor, puts into the glass sheet or the piezoid that are loaded with spongy graphene film after stirring, and 100-200 ℃ is reacted 5 ~ 20 hours;
5). be loaded in the spongy graphene film on glass sheet or piezoid surface after reacting with after distilled water flushing 3-6 time, put into muffle furnace after drying in baking oven, at 300-500 ℃ of lower roasting 1-5 hour, form functionalization three-dimensional sponge shape Graphene electrochemical electrode.
2. the preparation method of the three-dimensional grapheme electrode of electrochemica biological sensing according to claim 1, is characterized in that the spongy graphene film of described three-dimensional structure is the square or rectangular that the diameter disk that is 0.1 ~ 5cm or the length of side are 0.1 ~ 5cm.
3. the preparation method of the three-dimensional grapheme electrode of electrochemica biological sensing according to claim 1, is characterized in that described plain conductor, and material is copper, titanium or silver.
4. the application of the three-dimensional grapheme electrode of an electrochemica biological sensing as claimed in claim 1, is characterized in that described NOT-function three-dimensional sponge shape Graphene electrodes can carry out the Electrochemical Detection of dopamine, nicotinamide adenine dinucleotide and uric acid.
5. the application of the three-dimensional grapheme electrode of an electrochemica biological sensing as claimed in claim 1, is characterized in that described functionalization three-dimensional sponge shape Graphene electrochemical electrode can carry out detecting without enzyme of glucose.
6. the application of the three-dimensional grapheme electrode of electrochemica biological sensing according to claim 5, is characterized in that the selectivity that described functionalization three-dimensional sponge shape Graphene electrochemical electrode can carry out glucose, uric acid and ascorbic acid in blood plasma detects without enzyme.
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|---|---|---|---|---|
| CN103076375A (en) * | 2012-12-21 | 2013-05-01 | 吉林大学 | Preparation method and application of coaxial entity/nano porous gold/Co3O4 compound electrode material |
| CN103257168B (en) * | 2013-01-31 | 2015-04-22 | 浙江工业大学 | Acetylcholine esterase electrode adopting foam nickel as carrier, and applications thereof |
| CN103424447B (en) * | 2013-08-22 | 2015-05-13 | 中国科学院上海微***与信息技术研究所 | Nano-particle enhancement detection device based on non-modified monolayer graphene being used as working electrode and application thereof |
| CN104760949B (en) * | 2014-01-08 | 2017-04-05 | 奈创科技股份有限公司 | Graphene generating means |
| CN104062339B (en) * | 2014-07-09 | 2016-08-31 | 华南师范大学 | [Ru (tatp) 3] 2+ modified electrode and the method being used for detecting glucose in blood, uric acid and ascorbic acid thereof |
| CN104807861B (en) * | 2015-04-09 | 2017-05-24 | 山东师范大学 | Preparation method of spongy graphene-based stretchable gas sensor |
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| CN107462620B (en) * | 2017-08-23 | 2019-11-29 | 齐齐哈尔大学 | Based on graphene/ZnO/ nickel foam nanocomposite glucose sensor electrode |
| CN109239150B (en) * | 2018-08-07 | 2019-09-10 | 山东大学 | A kind of Co with high sensitivity3O4Porous nano-sheet non-enzymatic base glucose sensor and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101837972A (en) * | 2010-05-28 | 2010-09-22 | 南京邮电大学 | Graphene three-dimensional structure and preparation method thereof |
| CN102225331A (en) * | 2011-04-03 | 2011-10-26 | 长安大学 | Particle electrode catalyst filling material for three-dimensional electrodes and preparation method thereof |
-
2012
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101837972A (en) * | 2010-05-28 | 2010-09-22 | 南京邮电大学 | Graphene three-dimensional structure and preparation method thereof |
| CN102225331A (en) * | 2011-04-03 | 2011-10-26 | 长安大学 | Particle electrode catalyst filling material for three-dimensional electrodes and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| Biological and chemical sensors based on graphene materials;Yuxin Liu等;《Chem. Soc. Rev.》;20111205;第41卷;第2283-2307页 * |
| Chung-Wei Kung等.Cobalt oxide acicular nanorods with high sensitivity for the non-enzymatic detection of glucose.《Biosensors and Bioelectronics》.2011,第27卷(第1期),第125-131页. |
| Co3O4 nanocrystals on graphene as a synergistic catalyst for oxygen reduction reaction;Yongye Liang等;《NATURE MATERIALS》;20110807;第10卷;第780-786页 * |
| Cobalt oxide acicular nanorods with high sensitivity for the non-enzymatic detection of glucose;Chung-Wei Kung等;《Biosensors and Bioelectronics》;20110702;第27卷(第1期);第125-131页 * |
| Yongye Liang等.Co3O4 nanocrystals on graphene as a synergistic catalyst for oxygen reduction reaction.《NATURE MATERIALS》.2011,第10卷第780-786页. |
| Yuxin Liu等.Biological and chemical sensors based on graphene materials.《Chem. Soc. Rev.》.2011,第41卷第2283-2307页. |
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