CN105670978A - Preparation method of composite microecological preparation for pigs - Google Patents
Preparation method of composite microecological preparation for pigs Download PDFInfo
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Abstract
The invention relates to a preparation method of a composite microecological preparation for pigs. The composite microecological preparation is mainly prepared from Bacillus coagulans, Bacillus subtilis and Bifidobacterium. The composite microecological preparation has obvious growth promoting effects on baby pigs and fattening pigs, and can enhance immunity of the organisms and improve the environment of the pigsty.
Description
Technical field
The present invention relates to a kind of microbial ecological agent and belong to biofermentation category, be specifically related to a boar feed composite microecologic agent.
Background technology
In the past few decades, antibiotic was once made that very big contribution for the development of aquaculture, but also brought more drawback simultaneously. Along with antibiotic use, easily make animal produce drug resistance, cause the dosage in feedstuff to continue to increase, effect worse and worse, available medicine is also fewer and feweri. Antibiotic use can make the resistance of animal body reduce, and even causes the decline of production capacity, and the mankind are also constituted the threat of health by antibiotic residual simultaneously. Therefore finding antibiotic substitute in recent years and become the problem increasingly paid attention in animal husbandry, wherein microbial ecological agent becomes the emphasis of research.
Microbial ecological agent is also called probiotics, active bacteria formulation, feedstuff adds and can not only maintain the balance of microflora system in animal intestine gastropore, promote growth of animals or poultry, reduce the generation of disease, improve poultry daily gain, improve meat, moreover it is possible to kill latent pathogen, reduce the generation of ammonia and sulfur species, improve growth of animals or poultry environment. Exactly because the above-mentioned advantage of microbial ecological agent and nonhazardous, have no drug resistance, noresidue, cost low, so obtaining substantial amounts of use in animal husbandry.
At present, in production, the main microbial ecological agent used has two kinds, a kind of unitary type preparation formed for single Pseudomonas, and another kind is the composite bacteria preparation of multiple different Pseudomonas composition. In general, compound more can promote growth of animals or poultry and improve efficiency of feed utilization.
Summary of the invention
The invention provides the preparation of a kind of compound micro-ecological preparation, for promoting the formation of Intestinum Sus domestica road dominant microflora, keep gastrointestinal tract ecological balance, improve the speed of growth, improve meat quality. The present invention is achieved in that
(1), prepared by strain: with Bacillus coagulans (CTCC23316) no less than 3 × 109Cfu/L, bacillus subtilis (ATCC23857) are no less than 5 × 109Cfu/L, bacillus bifidus (ATCC15708) are no less than 2 × 109cfu/L。
(2), the preparation of fermentation medium: raw material by percentage to the quality, adds after mix homogeneously. Wheat bran 70～80%, bagasse 10～20%, Semen Maydis powder 1～3%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, manganese sulfate 0.2～0.5%, calcium carbonate 4%, sodium chloride 1%, carry out sterilizing after regulating pH to 7.0 ± 0.5, add distilled water regulating pondage 10%.
(3), inoculation and fermentation: first bacillus subtilis and Bacillus coagulans are pressed 1:1 volume mixture, it is inoculated in culture medium with the mass percent of culture medium 25% again, at 25 DEG C, aerobic fermentation carries out anaerobic fermentation 2～3 days after 3～5 days again, mass percent inoculum concentration with 10% accesses bacillus bifidus, being simultaneously introduced the Fructus Lycopersici esculenti juice of 5%, at 36 DEG C, anaerobic fermentation was terminated after 2～3 days.
Proportioning raw materials last in preparation process for above-mentioned culture medium is wheat bran 75%, bagasse 15%, Semen Maydis powder 3%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, manganese sulfate 0.3%, calcium carbonate 4%, sodium chloride 1%, the rear Fructus Lycopersici esculenti juice 5% added. Feeding micro-ecological preparation is obtained by above method.
Advantages of the present invention:
1, the method that the present invention mainly adopts twice inoculation, prepared by batch fermentation, first Bacillus coagulans and bacillus subtilis are mixed with aerobe fermentation carry out in a large number propagation, efficient-decomposition Organic substance, then it is changed into anaerobic fermentation and produces lactic acid, restrain varied bacteria growing, and then access, for second time, the growing environment that bacillus bifidus provides favourable.
2, the culture medium raw material wide material sources adopted, reasonable mixture ratio, be conducive to the growth of strain.
3, made microbial ecological agent is conducive to adding in feedstuff in this way, has special sour fragrance, can improve the feed intake of pig.
4, involved strain is all conducive to maintaining the microecological balance of intestinal, enhancing immunity, reduces stool odor, improves the growing environment of pig. Bacillus coagulans therein, because it is more weak in the adhesiveness of intestinal epithelial cell, exists so being generally difficult under field conditions (factors) in intestinal, only adds the preparation containing Bacillus coagulans, this bacterium could be allowed to give full play to its prebiotic effect in intestinal.
Detailed description of the invention
Embodiment 1: the preparation of seed culture medium:
1, the preparation of Bacillus coagulans
1.1 Bacillus coagulans are from Chinese microorganism strain preservation center CTCC23316.
The preparation of 1.2 bacterium solution
First prepare containing glucose 80g/L; Dried Corn Steep Liquor Powder 1.2g/L; (NH4) SO45g/L; MgSO4 7H2O0.4g/L; KH2PO40.4g/L; FeSO4 7H2O and MnSO4 H2O is made into 3g/L solution and takes 0.5ml; CaCO340g/L; The 1000ml seed culture medium of pH7.2 is by actication of culture, then amplification culture step by step.
The preparation of 1.3 fermentation medium
In 65L fermentation tank, bagasse be raw material by percentage to the quality: bagasse 10%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, calcium carbonate 4%, pH7.2.
1.4 count plates
Total viable count is calculated with dilution spread flat band method.
1.5 capital equipments
The desk-top pH meter pH211 of Shanghai Shu Xin instrument and meter company limited micro computer, SANYO company electro-heating standing-temperature cultivator MIR-262 and high-pressure steam sterilizing pan MLS-2420, Beijing Sai Duolisi balance company limited electronic balance PL303, Guo Hua enterprise constant temperature oscillator SHA-C, upper Nereid grand experimental facilities company limited vacuum drying oven DZF-6021 type, east, Zhenjiang biological engineering equipment and technology company limited 65L fermentation tank.
2, the preparation of bacillus subtilis
2.1 bacillus subtilises are from Chinese microorganism strain preservation center ATCC23857.
The preparation of 2.2 bacterium solution
Preparation is containing glucose 50g/L; Carnis Bovis seu Bubali cream 1.2g/L; Peptone 1.5g/L, (NH4)SO42g/L; MgSO4·7H2O1g/L; KH2PO40.5g/L; Regulate pH be the 1000ml seed culture medium of 7.0 by actication of culture, then amplification culture step by step.
The preparation of 2.3 fermentation medium
In 65L fermentation tank, wheat bran be raw material by percentage to the quality: wheat bran 20%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, pH7.2.
2.4 count plates
Total viable count is calculated with dilution spread flat band method.
2.5 capital equipments
The desk-top pH meter pH211 of Shanghai Shu Xin instrument and meter company limited micro computer, SANYO company electro-heating standing-temperature cultivator MIR-262 and high-pressure steam sterilizing pan MLS-2420, Beijing Sai Duolisi balance company limited electronic balance PL303, Guo Hua enterprise constant temperature oscillator SHA-C, upper Nereid grand experimental facilities company limited vacuum drying oven DZF-6021 type, east, Zhenjiang biological engineering equipment and technology company limited 65L fermentation tank.
3, the preparation of bacillus bifidus
3.1 bacillus bifiduss are from Chinese microorganism strain preservation center ATCC15708.
The preparation of 3.2 bacterium solution
Preparation is containing glucose 20g/L; Yeast extract powder 2g/L; Peptone 5g/L, NaCl2g/L; 5% cysteine 1g/L; Fructus Lycopersici esculenti leachate 400ml; Hepar siccatum 1g; Regulate pH be the 1000ml seed culture medium of 7.0 by actication of culture, then amplification culture step by step.
The preparation of 3.3 fermentation medium
In 65L fermentation tank, Semen Maydis pulp be raw material by percentage to the quality: Semen Maydis pulp 30%, Tomato juice 15%, hepar siccatum 1%, sodium chloride 1%, 5% cysteine 0.3%, pH7.0.
3.4 count plates
Total viable count is calculated with dilution spread flat band method.
3.5 capital equipments
The desk-top pH meter pH211 of Shanghai Shu Xin instrument and meter company limited micro computer, SANYO company electro-heating standing-temperature cultivator MIR-262 and high-pressure steam sterilizing pan MLS-2420, Beijing Sai Duolisi balance company limited electronic balance PL303, Guo Hua enterprise constant temperature oscillator SHA-C, upper Nereid grand experimental facilities company limited vacuum drying oven DZF-6021 type, east, Zhenjiang biological engineering equipment and technology company limited 65L fermentation tank.
Embodiment 2: prepare microbial ecological agent in this approach
1, strain is equipped with
Preparation Bacillus coagulans 3.1 × 109Cfu/L, bacillus subtilis 4.5 × 109Cfu/L, bacillus bifidus 1.1 × 109cfu/L。
2, medium preparing
By percentage to the quality, add after raw material mix homogeneously. Wheat bran 80%, bagasse 10%, Semen Maydis powder 3%, ammonium sulfate 1%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.3%, manganese sulfate 0.4%, calcium carbonate 4%, sodium chloride 1%, carry out sterilizing after regulating pH to 7.0 ± 0.5, adds distilled water regulating pondage 10%.
3, inoculation and fermentation
First bacillus subtilis and Bacillus coagulans are pressed 1:1 volume mixture, it is inoculated in culture medium with the mass percent of culture medium 20% again, at 25 DEG C, aerobic fermentation carries out anaerobic fermentation 3 days after 4 days again, mass percent inoculum concentration with 15% accesses bacillus bifidus, being simultaneously introduced the Fructus Lycopersici esculenti juice of 5%, at 36 DEG C, anaerobic fermentation was terminated after 3 days.
Embodiment 3: prepare microbial ecological agent in this approach
1, strain is equipped with
Preparation Bacillus coagulans 3 × 109Cfu/L, bacillus subtilis 5 × 109Cfu/L, bacillus bifidus 1.5 × 109cfu/L。
2, medium preparing
By percentage to the quality, add after raw material mix homogeneously. Wheat bran 70%, bagasse 20%, Semen Maydis powder 4%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, manganese sulfate 0.3%, calcium carbonate 3%, sodium chloride 1%, carry out sterilizing after regulating pH to 7.0 ± 0.5, adds distilled water regulating pondage 10%.
3, inoculation and fermentation
First bacillus subtilis and Bacillus coagulans are pressed 1:1 volume mixture, it is inoculated in culture medium with the mass percent of culture medium 25% again, at 25 DEG C, aerobic fermentation carries out anaerobic fermentation 3 days after 5 days again, mass percent inoculum concentration with 10% accesses bacillus bifidus, being simultaneously introduced the Fructus Lycopersici esculenti juice of 5%, at 36 DEG C, anaerobic fermentation was terminated after 2 days.
Embodiment 4: prepare microbial ecological agent in this approach
1, strain is equipped with
Preparation Bacillus coagulans 2.8 × 109Cfu/L, bacillus subtilis 5 × 109Cfu/L, bacillus bifidus 8 × 108cfu/L。
2, medium preparing
By percentage to the quality, add after raw material mix homogeneously. Wheat bran 75%, bagasse 15%, Semen Maydis powder 4%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.4%, manganese sulfate 0.4%, calcium carbonate 3%, sodium chloride 0.8%, carry out sterilizing after regulating pH to 7.0 ± 0.5, adds distilled water regulating pondage 10%.
3, inoculation and fermentation
First bacillus subtilis and Bacillus coagulans are pressed 1:1 volume mixture, it is inoculated in culture medium with the mass percent of culture medium 25% again, at 25 DEG C, aerobic fermentation carries out anaerobic fermentation 3 days after 5 days again, mass percent inoculum concentration with 10% accesses bacillus bifidus, being simultaneously introduced the Fructus Lycopersici esculenti juice of 5%, at 36 DEG C, anaerobic fermentation was terminated after 2 days.
Embodiment 5: pig farm is tested
In animal science institute of Agricultural University Of Nanjing, product effect is tested. What test was chosen is that 7 age in days pigletss start test, and during 35 ages in days, test group pig improves 132g than matched group average daily gain, shows good growth-promoting effect.
Growing and fattening pigs have been carried out product testing by Farming Ltd. of Suzhou City, and average daily gain height 0.154kg compared with matched group, simultaneously to NH in pig house3、H2S measures, it has been found that the concentration of test group significantly reduces, and stink is decreased obviously.
Anhui group adds this product in middle hog grower feed, finishing period and fatten period average daily gain 0.734kg, feed-weight ratio 2.37, death rate are 0, are superior to matched group, it is shown that better effects.
Above specific embodiments of the invention being described in detail, but it is intended only as example, the present invention is not restricted to particular embodiments described above. To those skilled in the art, any equivalent modifications that the present invention is carried out and replacement are also all among scope of the invention. Therefore, in the impartial conversion made without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (5)
1. the preparation method of a boar compound micro-ecological preparation, its feature has claimed below: prepared by strain: with Bacillus coagulans (CTCC23316) no less than 3 × 109Cfu/L, bacillus subtilis (ATCC23857) are no less than 5 × 109Cfu/L, bacillus bifidus (ATCC15708) are no less than 2 × 109cfu/L。
2. the preparation method of a boar compound micro-ecological preparation as claimed in claim 1, the preparation of fermentation medium: raw material by percentage to the quality, adds after mix homogeneously.
3. wheat bran 70～80%, bagasse 10～20%, Semen Maydis powder 1～3%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, manganese sulfate 0.2～0.5%, calcium carbonate 4%, sodium chloride 1%, carry out sterilizing after regulating pH to 7.0 ± 0.5, add distilled water regulating pondage 10%.
4. the preparation method of a boar compound micro-ecological preparation as claimed in claim 1, inoculation and fermentation: first bacillus subtilis and Bacillus coagulans are pressed 1:1 volume mixture, it is inoculated in culture medium with the mass percent of culture medium 25% again, at 25 DEG C, aerobic fermentation carries out anaerobic fermentation 2～3 days after 3～5 days again, mass percent inoculum concentration with 10% accesses bacillus bifidus, being simultaneously introduced the Fructus Lycopersici esculenti juice of 5%, at 36 DEG C, anaerobic fermentation terminates after 2～3 days to obtain.
5. the preparation method of a boar compound micro-ecological preparation as claimed in claim 1, it is characterized in that: wheat bran 75%, bagasse 15%, Semen Maydis powder 3%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, manganese sulfate 0.3%, calcium carbonate 4%, sodium chloride 1% in medium preparing, the rear Fructus Lycopersici esculenti juice 5% added.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106544299A (en) * | 2016-11-04 | 2017-03-29 | 中北大学 | A kind of composite bacteria agent capable of prevention ruminant oxalism and preparation method and application |
| CN109329622A (en) * | 2018-10-30 | 2019-02-15 | 长沙拜特生物科技研究所有限公司 | Microbial inoculum containing bacillus coagulans and the application for spending pig to cultivate in Ningxiang |
Citations (3)
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| CN1682599A (en) * | 2004-04-14 | 2005-10-19 | 中国农业大学 | Microbial fodder additive and its preparing method and use |
| CN101803682A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Compound microbial agent and production method and application thereof |
| CN103988977A (en) * | 2014-05-28 | 2014-08-20 | 河南牧业经济学院 | Feed microecological preparation and preparation method thereof |
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2016
- 2016-04-06 CN CN201610208399.1A patent/CN105670978A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1682599A (en) * | 2004-04-14 | 2005-10-19 | 中国农业大学 | Microbial fodder additive and its preparing method and use |
| CN101803682A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Compound microbial agent and production method and application thereof |
| CN103988977A (en) * | 2014-05-28 | 2014-08-20 | 河南牧业经济学院 | Feed microecological preparation and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106544299A (en) * | 2016-11-04 | 2017-03-29 | 中北大学 | A kind of composite bacteria agent capable of prevention ruminant oxalism and preparation method and application |
| CN106544299B (en) * | 2016-11-04 | 2019-07-12 | 中北大学 | A kind of composite bacteria agent and the preparation method and application thereof preventing ruminant oxalism |
| CN109329622A (en) * | 2018-10-30 | 2019-02-15 | 长沙拜特生物科技研究所有限公司 | Microbial inoculum containing bacillus coagulans and the application for spending pig to cultivate in Ningxiang |
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Application publication date: 20160615 |