CN105663442A - Fermenting method of tonifying traditional Chinese medicine extracting solution - Google Patents
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- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention provides a fermenting method of a tonifying traditional Chinese medicine extracting solution, comprising: preparing the traditional Chinese medicine extracting solution, preparing a culture medium of the traditional Chinese medicine extracting solution, inoculating and fermenting. A tonifying traditional Chinese medicine extracting technology and microorganism fermentation are combined, and the usage of a traditional Chinese medicine is reduced and is reduced by 50-70%; after the traditional Chinese medicine extracting solution is extracted, the precipitation capacity is reduced, a storage life is prolonged and prolonged by 14.5-15 days; after the traditional Chinese medicine extracting solution is fermented, the traditional Chinese medicine is fast to absorb and short in medicine taking time, and after the traditional Chinese medicine extracting solution is fermented, content of effective components is improved and improved by 92% or more.
Description
Technical field
The invention belongs to the field of Chinese medicines, in particular to a kind of method adopting microorganism fermentation process to process Chinese medicine extract.
Background technology
Tonification apoplexy due to endogenous wind medical instrument has auxiliary human righteousness, improve infirmity, improve the effect of resistance against diseases, for health preserving, diseases prevention, curing the disease has great importance, especially in the prevention of disease, husbandry sector uses frequently as immunomodulator, common tonification apoplexy due to endogenous wind medicine includes, the Radix Astragali, Radix Ginseng, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Et Caulis Acanthopanacis Senticosi, Radix Notoginseng, Poria, Herba Epimedii, Radix Morindae Officinalis, Rhizoma Curculiginis, Fructus Psoraleae, Fructus Lycii, Cordyceps, Radix Rehmanniae Preparata, Radix Polygoni Multiflori Preparata, the Radix Paeoniae Alba, Rhizoma Chuanxiong, the Fructus Evodiae, Fructus Schisandrae Chinensis, Radix Aconiti Lateralis Preparata, Radix Ophiopogonis etc.
Fermentation technique refers to that people utilize the Fermentation of microorganism, and application technology means control sweat, the technology of large-scale production fermented product, now extensive use in food, health product, feedstuff interpolation, pharmacy etc. produce.
Existing tonification class Chinese medicine extraction liquid has the disadvantage that
(1) tonification class Chinese medicine adopts decocting technique to extract, and perishable in depositing process, precipitation is many, and the holding time is short;
(2) Chinese medicine makes consumption big;
(3) Chinese medicine is in intestinal, it is necessary to be decomposed to form after micromolecular compound through enteric microorganism, just can be absorbed by organisms utilization, and therefore, medicine time is long, absorbs slow.
Summary of the invention
For solving above-mentioned technical problem, the present invention provides the fermentation process of a kind of tonification class Chinese medicine extraction liquid, to realize following goal of the invention:
(1), after Chinese medicine extraction liquid is fermented by the present invention, the holding time is long, is unlikely to deteriorate;
(2), after Chinese medicine extraction liquid is fermented by the present invention, reduce Chinese medicine and make consumption;
(3), after Chinese medicine extraction liquid is fermented by the present invention, Chinese medicine absorbs fast.
For solving above-mentioned technical problem, the technical scheme that the present invention takes is as follows:
The fermentation process of a kind of tonification class Chinese medicine extraction liquid, including preparing Chinese medicine extraction liquid, preparation Chinese medicine extraction liquid culture medium, inoculation, fermentation.
The following is the further improvement to technique scheme:
Described Chinese medicine extraction liquid of preparing, tonification class Chinese medicine is after water boiling and extraction, and filtering and concentrating is to according to crude drug meter 0.5～1g/L, and preparation becomes Chinese medicine extraction liquid.
Described preparation Chinese medicine extraction liquid culture medium, by 1L Chinese medicine extraction liquid, 30～200ml potato culture, 0.3～1g carbamide, 0.5～1g potassium dihydrogen sulfate, 0.02～0.1g vitamin b2,0.02～0.1g vitamin b12 mixes, regulate pH value between 6.5～7.5,121 DEG C of sterilizing 30min, Preservation in sterile condition, preparation becomes Chinese medicine extraction liquid culture medium.
Described inoculation, is inoculated in the Chinese medicine extraction liquid culture medium of sterilizing by seed liquor according to the inoculum concentration of 8～20%;
Wherein said seed liquor consists of: aspergillus niger 109～1010Individual/ml, Filamentous fungi 109～1010Individual/ml, fiber actinomycetes 108～109Individual/ml, bacillus subtilis 1010～1011Individual/ml, enterococcus faecalis 1010～1011Individual/ml.
Described fermentation, fermentation temperature is 21～38 DEG C, stirring fermentation 30h～120h, and rotating speed is 10～30r/min, and after fermentation ends, sterilizing again, encapsulation preserves.
Compared with prior art, the invention have the benefit that
(1) tonification class Chinese medicine extractive technique is combined by the present invention with fermentable, reduces Traditional Chinese Medicine dosage, and Traditional Chinese Medicine dosage reduces 50-70%;
(2) Chinese medicine extraction of the present invention fermented after, precipitation capacity reduce, storage life extend, the holding time extend 14.5-15 days;
(3), after Chinese medicine extraction liquid is fermented by the present invention, Chinese medicine absorbs fast, and medicine time is short;
(4) after Chinese medicine extraction liquid is fermented by the present invention, improving active constituent content, active constituent content improves more than 50%.
Detailed description of the invention
Below by way of specific embodiment, the present invention is described, but the present invention is not merely defined in these embodiments.
The fermentation process of 1 one kinds of tonification class Chinese medicine extraction liquid of embodiment
(1) Chinese medicine extraction liquid is prepared
Tonification class Chinese medicine (Radix Astragali, Radix Angelicae Sinensis, Fructus Lycii, the Radix Paeoniae Alba) is after water boiling and extraction, and filtering and concentrating is to according to crude drug meter 0.5g/mL, and preparation becomes Chinese medicine extraction liquid.
The described Radix Astragali, Radix Angelicae Sinensis, Fructus Lycii, the Radix Paeoniae Alba, weight ratio is: 1:1:1:1.
(2) Chinese medicine extraction liquid culture medium is prepared
By 1L Chinese medicine extraction liquid, 30ml potato culture, 0.3g carbamide, 1g potassium dihydrogen sulfate, 0.1g vitamin b2, 0.1g vitamin b12, regulating pH value 6.5, mixing, 121 DEG C of sterilizing 30min, Preservation in sterile condition, preparation becomes Chinese medicine extraction liquid culture medium.
(3) inoculation
Seed liquor is inoculated in the Chinese medicine extraction liquid culture medium of sterilizing according to the inoculum concentration of 8%;
Wherein said seed liquor consists of: aspergillus niger 109//ml, Filamentous fungi 109Individual/ml, fiber actinomycetes 108Individual/ml, bacillus subtilis 1010Individual/ml, enterococcus faecalis 1011Individual/ml.
(4) fermentation
Fermentation temperature is 21～25 DEG C, stirring fermentation 120h, and rotating speed is 10r/min, and after fermentation ends, sterilizing again, encapsulation preserves.
Technique effect:
Chinese medicine extraction fermentation liquid prepared by this method is compared with the Chinese medicine extraction liquid after unfermentable sterilizing, holding time on average extends 15 days, when the non-adding preservative agent of Chinese medicine extraction liquid preserves 15 days, all become mildewed, flatulence phenomenon, Chinese medicine extraction fermentation liquid can preserve 31 days, just occurs becoming mildewed, the phenomenon such as flatulence.
By the Chinese medicine extraction fermentation liquid prepared in embodiment 1 and Chinese medicine extraction liquid, add at broiler brood time and use, find: the drinking-water addition of Chinese medicine extraction fermentation liquid 1:1000 prepared by this method and the drinking-water consumption of Chinese medicine extraction liquid culture matrix 1:300, in promoting broiler Development of Immune Organs, effect is identical, after Chinese medicine extraction liquid of the present invention fermentation, the consumption of broiler brood time reduces by 70%.
The fermentation process of 2 one kinds of tonification class Chinese medicine extraction liquid of embodiment
(1) Chinese medicine extraction liquid is prepared
Tonification class Chinese medicine (Radix Codonopsis, Herba Epimedii, Radix Rehmanniae Preparata) is after water boiling and extraction, and filtering and concentrating is to according to crude drug meter 1g/mL, and preparation becomes Chinese medicine extraction liquid.
Described Radix Codonopsis, Herba Epimedii, Radix Rehmanniae Preparata weight ratio be 1:1:1.
(2) Chinese medicine extraction liquid culture medium is prepared
By 1L Chinese medicine extraction liquid, 200ml potato culture, 1g carbamide, 0.5g potassium dihydrogen sulfate, 0.02g vitamin b2, 0.02g vitamin b12Mixing, regulating pH value is 7.5,121 DEG C of sterilizing 30min, Preservation in sterile condition, and preparation becomes Chinese medicine extraction liquid culture medium.
(3) inoculation
Seed liquor is inoculated in the Chinese medicine extraction liquid culture medium of sterilizing according to the inoculum concentration of 20%;
Wherein said seed liquor consists of: aspergillus niger 1010Individual/ml, Filamentous fungi 1010Individual/ml, fiber actinomycetes 109Individual/ml, bacillus subtilis 1010Individual/ml, enterococcus faecalis 1010Individual/ml.
(4) fermentation
Fermentation temperature is 35～38 DEG C, stirring fermentation 120h, and rotating speed is 30r/min, and after fermentation ends, sterilizing again, encapsulation preserves.
Technique effect:
Chinese medicine extraction fermentation liquid prepared by this method is compared with the Chinese medicine extraction liquid after unfermentable sterilizing, holding time on average extends 14.5 days, the non-adding preservative agent preservation of Chinese medicine extraction liquid is all become mildewed for 16 days, flatulence phenomenon, Chinese medicine extraction fermentation liquid can preserve 30 days, just occurs becoming mildewed, the corruption such as flatulence.
By the Chinese medicine extraction fermentation liquid prepared in embodiment 2 and Chinese medicine extraction liquid, add before broiler vaccine immunity and use, find: the drinking-water addition of Chinese medicine extraction fermentation liquid 1:800 prepared by this method and the drinking-water consumption of Chinese medicine extraction liquid culture matrix 1:400, identical in improving immune effect of vaccine; After Chinese medicine extraction liquid of the present invention fermentation, the consumption of broiler brood time reduces by 50%.
The fermentation process of 3 one kinds of tonification class Chinese medicine extraction liquid of embodiment
(1) Chinese medicine extraction liquid is prepared
Choosing tonification class Chinese medicine-Fructus Schisandrae Chinensis, after water boiling and extraction, filtering and concentrating is to according to crude drug meter 0.8g/mL, and preparation becomes Chinese medicine extraction liquid.
(2) Chinese medicine extraction liquid culture medium is prepared
By 1L Chinese medicine extraction liquid, 100ml potato culture, 0.6g carbamide, 0.8g potassium dihydrogen sulfate, 0.07g vitamin b2, 0.08g vitamin b12Mixing, regulates pH value at 7,121 DEG C of sterilizing 30min, Preservation in sterile condition, and preparation becomes Chinese medicine extraction liquid culture medium.
(3) inoculation
Seed liquor is inoculated in the Chinese medicine extraction liquid culture medium of sterilizing according to the inoculum concentration of 12%;
Wherein said seed liquor consists of: aspergillus niger 1010Individual/ml, Filamentous fungi 109Individual/ml, fiber actinomycetes 109Individual/ml, bacillus subtilis 1011Individual/ml, enterococcus faecalis 109Individual/ml.
(4) fermentation
Fermentation temperature is 28～32 DEG C, stirring fermentation 35h, and rotating speed is 20r/min, and after fermentation ends, sterilizing again, encapsulation preserves.
Chinese medicine extraction fermentation liquid prepared by this method is compared with the extracting solution after unfermentable sterilizing, holding time on average extends 15 days, the non-adding preservative agent preservation of Chinese medicine extraction liquid is all become mildewed for 15 days, flatulence phenomenon, when Chinese medicine extraction fermentation liquid can preserve 31 days, occur becoming mildewed, the corruption such as flatulence.
By the Chinese medicine extraction fermentation liquid prepared in embodiment 3 and Chinese medicine extraction liquid, it is respectively adopted the content of Syrups by HPLC schisandrin therein, in Chinese medicine extraction fermentation liquid, the content of schisandrin is 2.5mg/ml after measured, in Chinese medicine extraction liquid, the content of schisandrin is 1.3mg/ml, schisandrin is one of principle active component of Fructus Schisandrae Chinensis, there is hepatoprotective effect, by the content that can improve schisandrin that ferments.
The fermentation process of 4 one kinds of tonification class Chinese medicine extraction liquid of embodiment
(1) Chinese medicine extraction liquid is prepared
Choosing tonification class Chinese medicine Radix Codonopsis, after water boiling and extraction, filtering and concentrating is to according to crude drug meter 0.8g/mL, and preparation becomes Chinese medicine extraction liquid.
(2) Chinese medicine extraction liquid culture medium is prepared
By 1L Chinese medicine extraction liquid, 100ml potato culture, 0.6g carbamide, 0.8g potassium dihydrogen sulfate, 0.07g vitamin b2, 0.08g vitamin b12Mixing, regulates pH value at 7,121 DEG C of sterilizing 30min, Preservation in sterile condition, and preparation becomes Chinese medicine extraction liquid culture medium.
(3) inoculation
Seed liquor is inoculated in the Chinese medicine extraction liquid culture medium of sterilizing according to certain inoculum concentration;
Wherein said seed liquor consists of: aspergillus niger 1010Individual/ml, Filamentous fungi 109Individual/ml, fiber actinomycetes 109//ml, bacillus subtilis 1011Individual/ml, enterococcus faecalis 1010Individual/ml.
(4) fermentation
Under certain fermentation temperature, rotating speed, stirring fermentation, after fermentation ends, sterilizing again, encapsulation preserves.
Embodiment 5 inoculum concentration single factor analysis is tested
Adopt the method described in embodiment 4, (3) inoculation step, change the inoculum concentration of inoculation liquid, be described in table 1 below:
Table 1
Fermentation temperature is 30 DEG C, stirring fermentation 60h, and rotating speed is 25r/min, and after fermentation ends, sterilizing again, encapsulation preserves.
Chinese medicine extraction liquid after fermentation, adds experiment before carrying out holding time and broiler vaccine immunity, and result is table 2 below such as:
Table 2
Visible, embodiment 8 and 9 is preferred embodiment, and namely inoculum concentration is preferably 15-18%.
Embodiment 11 fermentation temperature single factor analysis is tested
Adopt the method described in embodiment 4, (4) fermentation step, change fermentation temperature, be described in table 3 below:
Table 3
Seed liquor inoculum concentration is 15%, stirring fermentation 60h, and rotating speed is 25r/min, and after fermentation ends, sterilizing again, encapsulation preserves.
Chinese medicine extraction liquid after fermentation, adds experiment before carrying out holding time and broiler vaccine immunity, and experimental result is table 4 below such as:
Table 4
Visible: embodiment 14 and embodiment 15 are preferred embodiments, and namely fermentation temperature is preferably 30-35 DEG C.
Embodiment 17 fermentation time single factor analysis is tested
Adopt the method described in embodiment 4, (4) fermentation step, change fermentation time, be described in table 5 below:
Table 5
Seed liquor inoculum concentration is 15%, and fermentation temperature is 30 DEG C, and rotating speed is 25r/min, and after fermentation ends, sterilizing again, encapsulation preserves.
Chinese medicine extraction liquid after fermentation, adds experiment before carrying out holding time and broiler vaccine immunity, and experimental result is table 6 below such as:
Table 6
Visible, embodiment 20 and embodiment 21 are preferred embodiments, and namely fermentation time is preferably 60-90h.
Unless specifically indicated, ratio of the present invention, it is mass ratio, described percentage ratio, is mass percent.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature carries out equivalent replacement. All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (7)
1. the fermentation process of a tonification class Chinese medicine extraction liquid, it is characterised in that: include preparing Chinese medicine extraction liquid, preparation Chinese medicine extraction liquid culture medium, inoculation, fermentation.
2. the fermentation process of a kind of tonification class Chinese medicine extraction liquid according to claim 1, it is characterised in that: described inoculation, seed liquor is inoculated in the Chinese medicine extraction liquid culture medium of sterilizing according to the inoculum concentration of 8～20%.
3. the fermentation process of a kind of tonification class Chinese medicine extraction liquid according to claim 2, it is characterised in that: described seed liquor, consist of: aspergillus niger 109～1010Individual/ml, Filamentous fungi 109～1010Individual/ml, fiber actinomycetes 108～109Individual/ml, bacillus subtilis 1010～1011Individual/ml, enterococcus faecalis 109～1011Individual/ml.
4. the fermentation process of a kind of tonification class Chinese medicine extraction liquid according to claim 1, it is characterised in that: described fermentation, fermentation temperature is 21～38 DEG C, and stirring fermentation 30h～120h, rotating speed is 10～30r/min.
5. the fermentation process of a kind of tonification class Chinese medicine extraction liquid according to claim 1, it is characterized in that: described preparation Chinese medicine extraction liquid culture medium, by 1L Chinese medicine extraction liquid, 30～200ml potato culture, 0.3～1g carbamide, 0.5～1g potassium dihydrogen sulfate, 0.02～0.1g vitamin b2, 0.02～0.1g vitamin b12Mixing, regulates pH value between 6.5～7.5,121 DEG C of sterilizing 30min, and Preservation in sterile condition, preparation becomes Chinese medicine extraction liquid culture medium.
6. the fermentation process of a kind of tonification class Chinese medicine extraction liquid according to claim 1, it is characterized in that: described prepare Chinese medicine extraction liquid, tonification class Chinese medicine is after water boiling and extraction, and filtering and concentrating is to according to crude drug meter 0.5～1g/L, and preparation becomes Chinese medicine extraction liquid.
7. the fermentation process of a kind of tonification class Chinese medicine extraction liquid according to claim 1, it is characterised in that: described tonification class Chinese medicine is: one or more in the Radix Astragali, Radix Angelicae Sinensis, Fructus Lycii, the Radix Paeoniae Alba, Radix Codonopsis, Herba Epimedii, Radix Rehmanniae Preparata, Fructus Schisandrae Chinensis.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107625095A (en) * | 2017-11-07 | 2018-01-26 | 广东生和堂健康食品股份有限公司 | A kind of Guiling Jelly and its preparation technology of effect of being dried with toxin expelling |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606991A (en) * | 2009-07-02 | 2009-12-23 | 北京联合大学生物化学工程学院 | The technology of preparing fugui ostealgia compound by microorganism fermentation method |
| CN104771563A (en) * | 2015-04-29 | 2015-07-15 | 岳阳新华达制药有限公司 | Kidney tonifying Chinese herbal medicine compound preparation and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606991A (en) * | 2009-07-02 | 2009-12-23 | 北京联合大学生物化学工程学院 | The technology of preparing fugui ostealgia compound by microorganism fermentation method |
| CN104771563A (en) * | 2015-04-29 | 2015-07-15 | 岳阳新华达制药有限公司 | Kidney tonifying Chinese herbal medicine compound preparation and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107625095A (en) * | 2017-11-07 | 2018-01-26 | 广东生和堂健康食品股份有限公司 | A kind of Guiling Jelly and its preparation technology of effect of being dried with toxin expelling |
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