CN105662959A - Natural plant composition with skin whitening effect and application thereof to cosmetics - Google Patents
Natural plant composition with skin whitening effect and application thereof to cosmetics Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a natural plant composition with a skin whitening effect and application thereof to cosmetics. The natural plant composition is prepared from, by mass, 0.01-20% of white mulberry root bark extract, 0.01-20% of rhizoma atractylodis extract, 0.01-20% of radix paeoniae alba extract, 0.01-20% of ginger root extract, 0.01-20% of Chinese yam extract and the balance water. According to the plant composition, efficient tyrosinase inhibiting activity of the white mulberry root bark extract, the rhizoma atractylodis extract and the radix paeoniae alba extract, excellent oxidation resistance and free radical scavenging activity of the ginger root extract and excellent moisture retention performance of the Chinese yam extract are combined organically, formation of melanin of the skin base layer is effectively reduced, the skin can be fairer, clearer and brighter, and the effect of whitening the base is achieved.
Description
Technical field
The present invention relates to a kind of vegetable composition, belong to cosmetic technical field, particularly relate to a kind of natural plant composition with white-skinned face function and the application in cosmetics thereof.
Background technology
Skin is the first line of defence of health, is also beautiful the first agent of appearance, has health, skin pale, whippy is the target that everyone pursues. The skin of fair and clear delicacy is always up Asians and weighs the major criterion of women, and China women always advocates " skin is as avenged, coagulating such as fat ", the saying more having " a white screening hundred is ugly " among the people. The critical role of whitening class cosmetics occuping market share always. At present, general skin-whitening agents is mainly by suppressing tyrosinase activity or blocking the tryrosinase melanic route of synthesis of production, thus reducing melanic production to reach skin whitening effect, this type of skin-whitening agents mainly has arbutin, kojic acid and derivant, nicotiamide, Vc ethylether, Trenaxmine etc. This single inhibiting mechanism adds the reasons such as active matter easily inactivates, osmotic effect is not good so that whitening effect does not far reach expection.
Therefore, combining the effective ingredient of two or more different mechanism of action strengthening whitening effect of upgrading further is the trend of whitening product development.
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, a kind of natural plant composition with white-skinned face function is provided, described compositions is by suppressing the different mechanisms of action such as tyrosinase activity, antioxidation, scavenging free radicals, moisturizing lock water, combined effect, thus reaching collaborative whitening effect.
Another object of the present invention is to provide and described a kind of there is white-skinned face function natural plant composition for preparing the application of cosmetics.
Above-mentioned purpose of the present invention is to be achieved by the following technical programs:
A kind of natural plant composition with white-skinned face function, by mass percentage, comprise the following ingredients: Cortex Mori extract: 0.01～20%, Rhizoma Atractylodis Macrocephalae extract: 0.01～20%, Radix Paeoniae Alba extract: 0.01～20%, ginger-root extract: 0.01～20%, Rhizoma Dioscoreae extract: 0.01～20%, surplus is water.
The natural plant composition with white-skinned face function of the present invention, also can be made up of the component of following mass percent:
Cortex Mori extract 0.1～15%, Rhizoma Atractylodis Macrocephalae extract 0.1～15%, Radix Paeoniae Alba extract 0.1～15%, ginger-root extract 0.1～15%, Rhizoma Dioscoreae extract 0.1～15%, surplus are water.
The natural plant composition with white-skinned face function of the present invention, also can be made up of the component of following mass percent:
Cortex Mori extract 20%, Rhizoma Atractylodis Macrocephalae extract 10%, Radix Paeoniae Alba extract 10%, ginger-root extract 15%, Rhizoma Dioscoreae extract 12%, surplus are water.
The present invention has the natural plant composition of white-skinned face function, also can be made up of the component of following mass percent:
Cortex Mori extract 10%, Rhizoma Atractylodis Macrocephalae extract 20%, Radix Paeoniae Alba extract 15%, ginger-root extract 20%, Rhizoma Dioscoreae extract 8%, surplus are water.
The present invention has the application in cosmetics of the natural plant composition of white-skinned face function, described natural plant extracts, and its consumption is calculated as with dried object: 0.01%～20%.
Application of the present invention, described natural plant extracts, its consumption is calculated as with dried object: 0.1%～10%, it is preferable that consumption is 0.5%～5%.
Application of the present invention, described toiletry bag is containing natural plant extracts; And the cosmetic field customary adjuvant of 80～99.9%.
Application of the present invention, described customary adjuvant comprises surfactant, thickening agent, conditioner, wetting agent, preservative, chelating agen, essence, deionized water etc.
Application of the present invention, described cosmetics can be made into various skin-protection product dosage forms such as including astringent, elite, facial treatment milk, skin moistening cream, facial cleansing agent, facial film, skin protection curry, end adornment.
Cortex Mori another name Cortex Mori, Radix Mori skin, Cortex Mori, Morus alba skin. Main product in Anhui, Henan, Zhejiang, Jiangsu, the ground such as Hunan. Cortex Mori extract can suppress tyrosinase activity preferably, reduces melanic formation, reaches the effect of whitening.
The Rhizoma Atractylodis Macrocephalae mainly originates in the ground such as Zhejiang, Anhui, Fujian. Recording according to relevant document, Rhizoma Atractylodis Macrocephalae extract has the stronger effect suppressing tyrosinase activity equally.
Radix Paeoniae Alba mainly originates in the ground such as Anhui, Heilungkiang, Jilin, Shanxi, Shaanxi, the Inner Mongol. Effect of the Radix Paeoniae Alba is spread to compare Chinese medicine beautifying method widely with effect, three plain soups that Ming Dynasty's medical literature " Elementary Medicine " is recorded, " Radix Paeoniae Alba, the Rhizoma Atractylodis Macrocephalae, each 5 grams of Poria, 2.5 grams of Radix Glycyrrhizae, decocting, decoction being taken warmly ". Typhoid fever fidgets due to deficiency treated at first by this prescription, later it is found that invigorating qi and benefiting blood, whitening and moistening skin, then comes at folklore. This side's compatibility is precise and appropriate, is suitable to pachylosis, sallow, chloasma, pigmentation etc. that QI and blood deficiency and coldness causes. Radix Paeoniae Alba extract has the effect suppressing tyrosinase activity equally.
Race, according to relevant bibliographical information, race contains gingerol, diarylheptanoids, has very strong antioxidation and effect of scavenging radical. According to old and feeble free-radical theory, in the aging course of skin, the infringement of radical pair skin is an important factor. The free radical of internal generation and fatty acid effect and generate the materials such as malonaldehyde, effect forms lipofuscin and is deposited on skin with the protein on cell membrane etc. for they, forms multiple mottle.
Containing substantial amounts of Chinese yam polysaccharide in Rhizoma Dioscoreae, mainly it is made up of rhamnose, arabinose, mannose, glucose and galactose, there is good moisturizing, lock water effect, make skin more add water tender, bright.
By technique scheme, the invention have the advantages that and beneficial effect:
1, the present invention has the compositions of white-skinned face function and has organically combined the performance of keeping humidity that Cortex Mori, the Rhizoma Atractylodis Macrocephalae, the antioxidation efficiently suppressing tyrosinase activity, ginger-root extract remarkable of Radix Paeoniae Alba, Free-radical scavenging activity and Rhizoma Dioscoreae extract are excellent, effectively reduce the melanic formation of skin base layer, make skin more fair and more clear, bright, reach the effect of substrate whitening.
2, lightening compositions is from moisturizing, antioxidation, scavenging free radicals, suppression tyrosinase activity many-side synergism, have while efficiently reducing melanin generation, enough moisture is supplemented to skin, promote Skin Cell vigor, scavenging free radicals, delaying cell aging, promote the metabolism of skin, reduce mottle to be formed, make that skin is more fair and more clear, bright, water is tender, reach True Beauty white effect.
Accompanying drawing explanation
Fig. 1 indicates that the tyrosinase activity of test example 1 hinders the chart of the result of test.
Fig. 2 indicates that the human body three-dimensional containing melanocyte of test example 1 cultivates the chart of the result suppressing melanin to produce in epidermis model test.
Detailed description of the invention
The following example further illustrates the various embodiments in the scope of the invention. These embodiments merely to illustration purpose and provide, be not used to limit the present invention because without departing from the spirit and scope of the present invention, it is possible to the present invention is carried out many changes.
In an embodiment of the present invention, described Cortex Mori extract, Rhizoma Atractylodis Macrocephalae extract, Radix Paeoniae Alba extract, ginger-root extract, Rhizoma Dioscoreae extract are commercially available. Following percentage ratio is weight/mass percentage composition.
Embodiment 1 (whitening compositional liquor)
The natural plant composition (I) with white-skinned face function is composed as follows:
Surplus is water.
Carry out mixing prepared natural plant composition extracting solution according to above-mentioned mass percent by above-mentioned each constituent respectively.
Embodiment 2 (whitening compositional liquor)
The natural plant composition (II) with white-skinned face function is composed as follows:
Carry out mixing prepared natural plant composition extracting solution according to above-mentioned mass percent by above-mentioned each constituent respectively.
Embodiment 3 (whitening compositional liquor)
The natural plant composition (III) with white-skinned face function is composed as follows:
Carry out mixing prepared natural plant composition extracting solution according to above-mentioned mass percent by above-mentioned each constituent respectively.
Following example are the cosmetics embodiment containing above-mentioned white-skinned face function natural plant composition.
Embodiment 4 (there is the skin whitener of white-skinned face function natural plant composition)
By above-mentioned formula, component A, component B being separately heated to 80 DEG C, be under agitation slowly added in component A by the mixture of component B so that it is emulsifying, continue stirring, add component C and component D when temperature is down to 50 DEG C, stirring is cooled to room temperature.
Embodiment 5 (there is the whitening emulsion of white-skinned face function natural plant composition)
By above-mentioned formula, component A, component B being separately heated to 80 DEG C, be under agitation slowly added in component A by the mixture of component B so that it is emulsifying, continue stirring, add component C and component D and component E when temperature is down to 50 DEG C, stirring is cooled to room temperature.
Embodiment 6 (there is the whitening lotion of white-skinned face function natural plant composition)
By above-mentioned formula, component A is put in emulsifying pot, fully dissolve when stirring, be then heated to system temperature and be about 80 DEG C; Stand and be incubated 20 minutes with sterilizing, then turn off heating, it is cooled to about 60 DEG C while stirring, by in component B hyaluronate sodium add glycerol, 1,3-butanediol is uniformly dispersed and puts into again in principal phase, adding component C, D, E in principal phase again when temperature is down to 45 DEG C, slowly stirring is cooled to room temperature.
Embodiment 7 (there is the whitening facial cleaning cream of white-skinned face function natural plant composition)
By above-mentioned, component A, B being separately heated to 80 DEG C, then under rapid stirring, be slowly added in A by B component, react 30min, be down to 60 DEG C, add component C, continue stirring cooling, be down to less than 45 DEG C and add component D, E, room temperature, discharging are down in stirring.
Compliance test result test example (whitening function evaluation methodology)
Test example 1
1, the mensuration of tryrosinase vigor is suppressed
With 3 × 104The cell density in individual cell/0.2ml/ hole is by B16 melanoma cells (melanomacell) sowing to 96 orifice plates, in the incubator of 37 DEG C, utilization is added with 10% hyclone (FetalBovineSerum, FCS), the DMEM culture medium (buying from GIBCO) of 1% antifungal antibiotic (Antibiotic-Antimycotic) (buying from GIBCO) is cultivated 24 hours.
Then, utilize the phosphate buffer (66mM of 100 μ L, pH is 6.8) by after cell cleaning, by the phosphate buffer (PhosphateBufferedSaline containing 1%TritonX-100, PBS) 100 μ L are added in each hole, then utilize the mixer of 37 DEG C to carry out 30 minutes dissolving.
After 30 minutes, supernatant 50 μ L is moved in the hole of new plate, add the 50 μ L being dissolved in PBS and be respectively verified medicine (comparative example: containing 2% arbutin; Embodiment contains the whitening vegetable composition described in 2% embodiment 1; On the other hand, prepare only to be added with the PBS hole without white-skinned face function material of 50 μ L, it can be used as matched group, between each sample, 5mML-DOPA solution 50 μ L is added rapidly in the way of not producing temporal difference, and utilize plate reader (PlateReader) to measure absorbance (475nm), it can be used as the amount of initial DOPA chromium (DOPAchrome).
Thereafter, one side utilizes the mixer of 37 DEG C to be stirred one side and preserves 45 minutes, thereafter, again measures absorbance (475nm), and measures the amount of the DOPAchrome after preservation, thus measures tyrosinase activity and is subject to great obstruction. According to the measured value obtained, calculate the obstruction rate (%) being added with when being respectively verified medicine when matched group is set to 100%. The results are shown in Fig. 1.
It will be seen from figure 1 that have good tyrosinase inhibitory action containing the whitening vegetable composition described in 2% embodiment 1, its effect is slightly better than traditional whitening agent arbutin of equal consumption, can as safe and effective whitening agent.
Test example 2
2, the mensuration of secondary ultra-oxygen anion free radical Scavenging activity
Take the Tris-HCl buffer 4.5mL of 0.05mol/LpH=8.2, in 25 DEG C of water-baths, preheat 20min. Adding the pyrogallol solution of 1mL sample and 0.4mL25mmol/L, after mixing, react 5min in 25 DEG C of water-baths, the HCl1.0mL adding 8mol/L terminates reaction. Make reference with Tris-HCl buffer, survey absorbance at 299nm place. Test sample is the whitening vegetable composition described in embodiment 1, and the solvent of blank 1mL sample substitutes sample. Clearance rate (D) is calculated according to formula (1). Experimental result is as shown in table 1.
Clearance rate formula (%) D=[1-(A2/A1)] × 100% (1)
In formula, A1 is the absorbance of blank; A2 is the absorbance of sample.
Test example 3
3, the mensuration to the Scavenging activity of hydroxy radical.
25mL color comparison tube is sequentially added into 2mmol/LFeSO43mL, 1mmol/LH2O23mL, shakes up, and is subsequently added into 6mmol/L salicylic acid 3mL, shakes up, and takes out, survey its absorbance after 37 DEG C of heating in water bath 15min; It is separately added into certain density liquid to be measured, shakes up, continue heating in water bath 15min, take out and survey its absorbance. Liquid sample to be measured is the whitening vegetable composition described in embodiment 1, and the solvent of blank 1mL sample substitutes sample.
Formula 2) for the liquid to be measured clearance rate to carboxylic free radical (OH). Free radical scavenging activity experimental result is as shown in table 1.
OH clearance rate (%)=(A3-A2)/A1 × 100% (2)
In formula, A1 does not add the absorbance of reaction system before medicine; A2 is the absorbance of system after sample removing OH; A3 is the absorbance of system after blank removing OH.
Table 1 natural plant composition free radical scavenging activity result
| Experimental project | Clearance rate (%) |
| To superoxide anion scavenging action | 49.81 |
| Scavenging action to hydroxy radical | 41.25 |
By table 1, we can see that natural plant composition of the present invention has good radical scavenging activity.
Test example 4
4, human body 3D skin model test melanocyte produces melanic assay method
This 3D model is owing to having the structure close to human normal skin, and containing melanocyte, is therefore the known model test useful for the skin basic medical research relevant to pigmentation etc. Specifically, in this test example, LabCyte.MELANO-MODEL24 is overlapped group (manufacture of JTEC company) and cultivates epidermis model as human body three-dimensional. Test the operating instruction basically according to this set group to carry out.
First, the improvement melanin together loaded to set group is utilized to produce to promote that the epidermis model of this set group is carried out 12 hours cultivating by culture medium. Thereafter, the 50 μ L being dissolved in PBS are respectively verified medicine (comparative example: containing 2% arbutin; Embodiment contains the whitening vegetable composition described in 2% embodiment 1) it is added into culture dish; On the other hand, prepare only to be added with the PBS hole without white-skinned face function material of 50 μ L, it can be used as matched group, every other day culture medium and each PBS solution are replaced. From starting for the exposure being verified medicine after 13 days, reclaim epidermis model, and carry out melanic quantitatively. Furthermore, melanin amount makes corrections according to the ratio of the viable count utilizing dimethyl diaminophenazine chloride (NeutralRed, NR) method to measure. The results are shown in Fig. 2.
From Fig. 2, we can see that, in using the test of 3D skin model, we draw equally, the whitening vegetable composition described in 2% embodiment 2 is used to have the effect well suppressing melanin to produce, its effect is apparently higher than the arbutin of equal consumption, and can form clean, bright skin form.
The above, it it is only presently preferred embodiments of the present invention, not the present invention is done any pro forma restriction, therefore it is every without departing from technical solution of the present invention content, according to any simple modification, equivalent variations and modification that above example is made by the technical spirit of the present invention, all still fall within the scope of technical solution of the present invention.
Claims (9)
1. a natural plant composition with white-skinned face function, it is characterised in that: it is made up of the component of following mass percent:
Cortex Mori extract 0.01～20%, Rhizoma Atractylodis Macrocephalae extract 0.01～20%, Radix Paeoniae Alba extract 0.01～20%, ginger-root extract 0.01～20%, Rhizoma Dioscoreae extract 0.01～20%, surplus are water.
2. the natural plant composition with white-skinned face function according to claim 1, it is characterised in that: it is made up of the component of following mass percent:
Cortex Mori extract 0.1～15%, Rhizoma Atractylodis Macrocephalae extract 0.1～15%, Radix Paeoniae Alba extract 0.1～15%, ginger-root extract 0.1～15%, Rhizoma Dioscoreae extract 0.1～15%, surplus are water.
3. the natural plant composition with white-skinned face function according to claim 1, it is characterised in that: it is made up of the component of following mass percent:
Cortex Mori extract 20%, Rhizoma Atractylodis Macrocephalae extract 10%, Radix Paeoniae Alba extract 10%, ginger-root extract 15%, Rhizoma Dioscoreae extract 12%, surplus are water.
4. the natural plant composition with white-skinned face function according to claim 1, it is characterised in that: it is made up of the component of following mass percent:
Cortex Mori extract 10%, Rhizoma Atractylodis Macrocephalae extract 20%, Radix Paeoniae Alba extract 15%, ginger-root extract 20%, Rhizoma Dioscoreae extract 8%, surplus are water.
5. the application in cosmetics of the natural plant composition with white-skinned face function according to any one of claim 1-4, it is characterised in that: described natural plant extracts, its consumption is calculated as with dried object: 0.01%～20%.
6. application according to claim 5, it is characterised in that: its consumption of described natural plant extracts is calculated as with dried object: 0.1%～10%, it is preferred to 0.5%～5%.
7. application according to claim 5, it is characterised in that: described toiletry bag is containing natural plant extracts; And the cosmetic field customary adjuvant of 80～99.9%.
8. application according to claim 5, it is characterised in that: described customary adjuvant comprises surfactant, thickening agent, conditioner, wetting agent, preservative, chelating agen, essence, deionized water etc.
9. application according to claim 5, it is characterised in that: described cosmetics can be made into various skin-protection product dosage forms such as including astringent, elite, facial treatment milk, skin moistening cream, facial cleansing agent, facial film, skin protection curry, end adornment.
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| CN106137851A (en) * | 2016-08-14 | 2016-11-23 | 广州市博真电子科技有限公司 | A kind of elite curry containing former Radix Ginseng and preparation method thereof |
| CN106821837A (en) * | 2016-11-01 | 2017-06-13 | 田鹏新 | A kind of skin cream of the medicament extract of the root of herbaceous peony containing centella and preparation method thereof |
| CN107823058A (en) * | 2017-11-29 | 2018-03-23 | 任超 | A kind of skin whitening cleaning agent and preparation method thereof |
| CN107823023A (en) * | 2017-12-21 | 2018-03-23 | 李冬梅 | A kind of natural plant extracts composition and its application in cosmetics |
| CN108210388A (en) * | 2018-02-12 | 2018-06-29 | 广东暨晴生物医药科技有限公司 | A kind of winter equilibrium water and its production method |
| CN108578261A (en) * | 2018-05-21 | 2018-09-28 | 德之馨（上海）有限公司 | A kind of water solubility whitening composite formula and its preparation method and application |
| CN111388367A (en) * | 2020-03-25 | 2020-07-10 | 江南大学 | Composition for inhibiting melanin at multiple target points, preparation method and application of composition in cosmetics |
| CN112807256A (en) * | 2020-03-23 | 2021-05-18 | 上海铮信生物科技有限公司 | Whitening composition and application thereof in cosmetics |
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| CN106137851A (en) * | 2016-08-14 | 2016-11-23 | 广州市博真电子科技有限公司 | A kind of elite curry containing former Radix Ginseng and preparation method thereof |
| CN106821837A (en) * | 2016-11-01 | 2017-06-13 | 田鹏新 | A kind of skin cream of the medicament extract of the root of herbaceous peony containing centella and preparation method thereof |
| CN107823058A (en) * | 2017-11-29 | 2018-03-23 | 任超 | A kind of skin whitening cleaning agent and preparation method thereof |
| CN107823023A (en) * | 2017-12-21 | 2018-03-23 | 李冬梅 | A kind of natural plant extracts composition and its application in cosmetics |
| CN108210388A (en) * | 2018-02-12 | 2018-06-29 | 广东暨晴生物医药科技有限公司 | A kind of winter equilibrium water and its production method |
| CN108578261A (en) * | 2018-05-21 | 2018-09-28 | 德之馨（上海）有限公司 | A kind of water solubility whitening composite formula and its preparation method and application |
| CN112807256A (en) * | 2020-03-23 | 2021-05-18 | 上海铮信生物科技有限公司 | Whitening composition and application thereof in cosmetics |
| CN111388367A (en) * | 2020-03-25 | 2020-07-10 | 江南大学 | Composition for inhibiting melanin at multiple target points, preparation method and application of composition in cosmetics |
| CN111388367B (en) * | 2020-03-25 | 2021-09-24 | 江南大学 | Composition for inhibiting melanin at multiple target points, preparation method and application of composition in cosmetics |
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