CN1056529A - The working method of macerating enzymic product and flax - Google Patents

The working method of macerating enzymic product and flax Download PDF

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Publication number
CN1056529A
CN1056529A CN90102756A CN90102756A CN1056529A CN 1056529 A CN1056529 A CN 1056529A CN 90102756 A CN90102756 A CN 90102756A CN 90102756 A CN90102756 A CN 90102756A CN 1056529 A CN1056529 A CN 1056529A
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Prior art keywords
flax
enzyme
product
macerating
present
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CN90102756A
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Chinese (zh)
Inventor
马连那·拉·皮特洛娃
尼·曼·亚那切娃
西·格·维什切加
多·勃·图帕什基
拉·尤·吉里娃
阿·皮·安那斯塔索娃
茨·托·萨诺瓦
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"VEDA" Firma
VEDA
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"VEDA" Firma
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Priority to DE4012351A priority Critical patent/DE4012351A1/en
Priority to FR9005422A priority patent/FR2661420A1/en
Application filed by "VEDA" Firma filed Critical "VEDA" Firma
Priority to CN90102756A priority patent/CN1056529A/en
Publication of CN1056529A publication Critical patent/CN1056529A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00—Treatment of vegetable material
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/685—Aspergillus niger

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Textile Engineering (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Paper (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Abstract

The present invention relates to the enzyme product and in flax primary processing in textile industry, flood the bast crop.
The present invention proposes a kind of prozyme, it can decompose protopectin, and solvable pectin substance and hemicellulose and do not destroy internal structure do not reduce the physical strength of fiber; A kind of method that flax raw material makes flax fiber that makes full use of has also been proposed.
The present invention realizes by the enzyme product that uses aspergillus niger Nepal 76-1 to produce.Behind the mechanical treatment, handle flax with this kind of enzyme mixture again.

Description

The working method of macerating enzymic product and flax
The present invention relates to biotechnology, promptly the enzyme product in the one-level of textile industry flax is handled, floods the bast crop.
Known macerating enzymic product (1), it is by clostridium felsineum 22(Clostridiumfelsineum22) and pectin fermentation clostridium 1(Clostridium Pectinofermenans 1) produce.
The shortcoming of this kind of enzyme product is that hydrolysis ability is low, active and pectin-anti-low-6,6 units per ml of enzymic activity of eliminating of lyase.
Known macerating enzymic product (2), it is by Bacillus circulans 31(Bacillus 31), spherical bacillus (Bacillussp.) produces, poly-galactase in comprising, poly-outward galactase, the pectin-anti-enzyme of eliminating.
The shortcoming of this product is that dipping is active low.
Known macerating enzymic product (3), it is by penicillium bacterial strain 24(Penicillum 24) produce poly-galactase in comprising, poly-galactase, the pectin-anti-enzyme of eliminating outward.
The pectic enzyme and the carbon black aspergillus tubigensis 77(Aspergillus Carbonarius 77 that utilize penicillium bacterial strain 24P to produce) producing the enzyme that has decomposition of cellulose and decompose the hemicellulose effect, to handle the method for flax be known.
The shortcoming of this known products and method is that enzymic activity is low, and the starting material loss is big, and the treatment time is long, and water and energy consumption expense are big, and causes the abominable of Working environment.Although can obtain long and short fiber, have only the macrofiber can be processed.
The objective of the invention is to prepare a kind of macerating enzymic product, it has the effect of enzyme complex, can destroy protopectin-, solvable pectin substance and hemicellulose and do not destroy the internal structure of fiber and do not reduce the physical strength of fiber; The invention allows for a kind of method of intactly using flax raw material to produce flax fiber.
This method can make process machineryization, and technical finesse is controlled, and can improve the working conditions of factory's flax primary processing, reduces the time of handling.
This purpose is by be registered as the aspergillus niger Nepal 76-1(Aspergiljus niger 76-1 of Nr 1333 at industrial microorganism and cell culture state vertical library) the enzyme product that produces realizes.Fermentation media is to make carbon and nitrogenous source by the waste material of foodstuffs industry, and somatomedin and mineral salt are elementary composition as other.At the incubation period of bacterial strain, 1000～12000 units/centimetre 3The galactosan enzyme, 15～20 units/centimetre 3Rohapect MPE, 1～2 unit/centimetre 3Protopectinase mixes and gathers in fermented liquid.Aspartic protease, beta-glucanase, glucoamylase can be used as the active cofactor of enhancing dipping and are present in the fermented liquid.The enzyme complex that is obtained can be used for the biochemistry decomposition that flax knot is formed thing.
Utilize the method for macerating enzymic product processing flax as follows:
Mechanical treatment flax stem branch is placed in the container subsequently, uses warm water soaking.The dosage that enzyme uses is 0.5～2% of fibre weight.3～4 hours treatment times.In order to increase contacting of enzyme and flax, liquid is circulated continuously, waste water also can be reused (4～5 times).In each container subsequently, add a spot of enzyme and macerate soft flax and the enzyme that loses with the reduction that replenishes enzymic activity and in taking-up.The dry flax fiber of handling with enzyme places it in pre-comb on the coarse tow equipment, subsequently again through the carding machine combing.
Present method is applicable to the factory of flax primary processing, and need not change equipment.
Product of the present invention and method advantage are as follows:
Aspergillus niger belongs to Nepal 76-1 bacterial strain to be cultivated on the substratum of cheapness, produces a kind of pectic enzyme, protease and cellulolytic enzyme mixture, is suitable for the production of flax fiber.Product can liquid or solid-state form flood flax, and flax raw material can make full use of.Can realize the mechanize that flax produces.The present invention can reduce average handling time significantly, increases the throughput of factory and improves working conditions.
The following example is used to illustrate the present invention, but never means the restriction to its scope.
Embodiment 1
Aspergillus niger Nepal 76-1(Aspergillus niger 76-1) cultivated 20 days, grow on inclined-plane malt agar base, make a kind of suspension with it, fermentation media is with 5 * 10 4÷ 1 * 10 5Spore/centimetre 3Inoculation.When fermentation ends, contain 12000 ± 300 unit galactosan enzymes in every cubic centimetre of liquid, 15 ± 5 unit Rohapect MPEs and 1 ± 1,5 unit protopectinases.Behind filtration and the centrifugal granule of removing in the medium and insolubles, ultrafiltration cleaning concentrated mother liquor 10 times.
Obtain a kind of prozyme product, contain 80000～100000 units/centimetre 3The galactosan enzyme, 160～180 units/centimetre 3Rohapect MPE, 10～12 units/centimetre 3Protopectinase and a certain amount of auxiliary enzymes-pepsin, beta-glucanase, glucoamylase, cellulase, they can increase the dipping activity of product.After the nutrient solution drying that is obtained, active as follows: 500000-700000 units/gram galactosan enzyme, 500-700 units/gram Rohapect MPE 70-120 units/gram protopectinase.
The amount of the required enzyme product of flax dry is decided by galactosan enzymic activity-1000～5000 units/gram flax raw material.
Flax stem is processed in peeling machine.The uniform fiber that is obtained contains 10～13% impurity, soaks in warm water, after the temperature regulation to 40 ℃, the enzyme product is joined in the soaking container.(general 3～5 hours-this depend on the matter of flax and the amount of enzyme) guarantee to circulate continuously in the steeping process.Subsequently, flax of handling well is washed three times drying, pre-comb, combing with warm water (60 ℃).After machining, the impurity of flax fiber finished product is less than 1%.

Claims (2)

1, the macerating enzymic product for preparing by aspergillus niger Nepal 76-1 (Aspegillus niger 76-1) [state-run industrial microorganism and cell culture storehouse registration-Nr 1333], has following activity: galactosan enzyme, Rohapect MPE, beta-glucanase, glucoamylase, cellulase.
2, the method for enzymatically treating of flax fiber is characterized in that handling the flax of peeling with the maceration enzyme in the claim 1, and wherein the amount of enzyme use is 0.5～2% of a raw material, 39～43 ℃ of temperature, and in 3～5 hours treatment times, condition is liquid continuous circulation.
Reference
1.A.C.SSSR Nr 622838，1978
2.A.C.SSSR Nr 1017728，1983
3.A.C.SSSR Nr 834271，1981
CN90102756A 1990-04-18 1990-05-14 The working method of macerating enzymic product and flax Pending CN1056529A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE4012351A DE4012351A1 (en) 1990-04-18 1990-04-18 Macerating enzyme prepn. obtd. from Aspergillus niger - contains polygalacturonase, pectin-esterase and proto-pectinase, which are used in the work up of flax
FR9005422A FR2661420A1 (en) 1990-04-18 1990-04-27 Enzymatic product of maceration and process for the treatment of linseed using this product
CN90102756A CN1056529A (en) 1990-04-18 1990-05-14 The working method of macerating enzymic product and flax

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4012351A DE4012351A1 (en) 1990-04-18 1990-04-18 Macerating enzyme prepn. obtd. from Aspergillus niger - contains polygalacturonase, pectin-esterase and proto-pectinase, which are used in the work up of flax
CN90102756A CN1056529A (en) 1990-04-18 1990-05-14 The working method of macerating enzymic product and flax

Publications (1)

Publication Number Publication Date
CN1056529A true CN1056529A (en) 1991-11-27

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CN90102756A Pending CN1056529A (en) 1990-04-18 1990-05-14 The working method of macerating enzymic product and flax

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DE (1) DE4012351A1 (en)
FR (1) FR2661420A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101550606B (en) * 2008-04-01 2011-10-26 湖南逐鹿苎麻纺织有限公司 Ramie degumming method utilizing a complex enzyme preparation
CN106661825A (en) * 2014-07-31 2017-05-10 爱贝克思集团控股公司 Method for producing hemp fiber for spinning, and hemp fiber for spinning
CN115029795A (en) * 2022-07-14 2022-09-09 杭州惠邦纺织有限公司 Ramie fiber moisture-absorption quick-drying fabric and preparation method thereof

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2699192B1 (en) * 1992-12-11 1995-05-12 Louis Hurdequint Enzymatic retting process controlling bast fibers and enzymatic retting solution.
FR2713671B1 (en) * 1993-12-15 1996-03-01 Sofilin Sa Enzymatic retting process.
DE69520951T2 (en) * 1994-10-28 2001-11-22 Novozymes A/S, Bagsvaerd METHOD FOR CHEMICAL FINISHING INSOLUBLE POLYMER FIBERS
EP0975725B2 (en) 1997-04-09 2009-02-11 Kao Corporation Detergent composition
DE19854324B4 (en) * 1998-09-09 2005-01-20 Herres-Costard, Cornelia Process for the biological recovery of cellulose fiber material from calcium pectinate-containing, vegetable substrate
ES2303506T3 (en) 1999-03-22 2008-08-16 Novozymes A/S ENZYMATIC TREATMENT METHOD.
JP6335306B2 (en) * 2014-07-31 2018-05-30 エイベックス株式会社 Hemp fiber for spinning and hemp fiber for spinning

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101550606B (en) * 2008-04-01 2011-10-26 湖南逐鹿苎麻纺织有限公司 Ramie degumming method utilizing a complex enzyme preparation
CN106661825A (en) * 2014-07-31 2017-05-10 爱贝克思集团控股公司 Method for producing hemp fiber for spinning, and hemp fiber for spinning
CN115029795A (en) * 2022-07-14 2022-09-09 杭州惠邦纺织有限公司 Ramie fiber moisture-absorption quick-drying fabric and preparation method thereof
CN115029795B (en) * 2022-07-14 2024-01-26 杭州惠邦纺织有限公司 Ramie fiber moisture-absorbing quick-drying fabric and preparation method thereof

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Publication number Publication date
FR2661420A1 (en) 1991-10-31
DE4012351A1 (en) 1991-10-24

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