CN106811489A - A kind of open production method of lectic acid of the lactic acid bacteria based on lactic acid producing pediocin - Google Patents
A kind of open production method of lectic acid of the lactic acid bacteria based on lactic acid producing pediocin Download PDFInfo
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- CN106811489A CN106811489A CN201510854665.3A CN201510854665A CN106811489A CN 106811489 A CN106811489 A CN 106811489A CN 201510854665 A CN201510854665 A CN 201510854665A CN 106811489 A CN106811489 A CN 106811489A
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Abstract
The present invention discloses a kind of open production method of lectic acid of the lactic acid bacteria based on lactic acid producing pediocin.Its tool is as follows：(1) lignocellulosic material is pre-processed；(2) mortifier produced in removing lignocellulosic preprocessing process；(3) without being sterilized with nutrients to lignocellulosic material, fermentation reactor, fermentation, directly to adding cellulase, lignocellulosic material and Pediococcus acidilactici Pediococcus acidilactici TY112 (CGMCC 8664) to synchronize saccharification and fermentation or substep saccharification and fermentation production of L-lactic acid in fermentation reactor, it is not required to keep germ-free condition in lactic acid production process.The present invention effectively reduces the sterilizing cost in cellulose lactic acid production process, simplifies the Operating Complexity of cellulose lactic acid production process.
Description
Technical field
It is a kind of open production method of lectic acid of the lactic acid bacteria based on lactic acid producing pediocin the present invention relates to biorefinery of lignocellulose field.
Background technology
Pfansteihl is very important bulk chemical, is widely used in the industries such as food, medicine, preventing and treating, environmental protection.Currently the most important ones purposes is the degradation plastic-PLA (Polymeric lactic acid, PLA) for synthetic environment-friendly.The lactic acid in the whole world about more than 90% is produced by microbial fermentation, but microbial fermentation produces the relatively costly of lactic acid, is mainly reflected in two aspects：First, starch or saccharide substrates high account for the 70% of lactic product production cost；Second, running cost high, including disinfecting action and later stage isolate and purify cost.
In order to improve the economy of lactic acid production process, it is a kind of very effective means to substitute the grain raw materials such as starch using abundant, cheap lignocellulosic material.And for second aspect, open lactic fermentation is the available strategy for reducing lactic acid production process energy consumption and production cost, the concern and attention of more and more researchers have been subjected at present.But, the lactic fermentation of current open formula can be fermented in normal growth under higher temperature conditions based on high temperature resistant lactic acid fermenting microbe, and high temperature is while what the mechanism that can suppress miscellaneous bacteria growth of microorganism was carried out.For example, using resistant to elevated temperatures bacillus coagulans Bacillus coagulans be 50 DEG C in temperature under conditions of to carry out open lactic fermentation be current most common method.But this method be generally all with saccharic or starch materials as substrate, and the cellulosic substrate of complexity do not carry out also it is authenticated.Additionally, the fermentation optimum pH of bacillus coagulans is 7.0 or so, this is larger with the difference of peak enzymolysis-ability pH value 4.8 of cellulase.Based on this, with lignocellulosic as substrate, the technique that can only be saccharified with fermentation using distribution when producing lactic acid using resistant to elevated temperatures bacillus coagulans, and can not be using the synchronous saccharification and zymotechnique of current main-stream.This will be significantly increased the Operating Complexity and production cost of cellulose lactic acid production process.Therefore, lactic fermentation production is carried out by substrate of lignocellulosic, it is necessary to develop a kind of new open type fermented process to reduce the production process complexity and production cost of cellulose lactic acid.
The content of the invention
It is an object of the invention to overcome the shortcomings of that existing open zymotechnique of lactic acid is not suitable for lignocellulosic synchronous saccharification with fermentation.This method can be greatly reduced the cost of lignocellulosic synchronous saccharification and fermenting lactic acid process, especially fermentation reactor, fermentation nutrients, the sterilizing energy consumption of lignocellulosic substrate and other items, and without maintaining germ-free condition in fermentation process, so as to greatly reduce the Operating Complexity of process, ensure lignocellulosic be converted into lactic acid yield it is constant under conditions of, the production cost of cellulose lactic acid is greatly reduced.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of open production method of lectic acid of the lactic acid bacteria based on lactic acid producing pediocin, it is characterised in that concretely comprise the following steps, (1) pre-processes to lignocellulosic material；(2) mortifier produced in removing lignocellulosic preprocessing process；(3) without being sterilized with nutrients to lignocellulosic material, fermentation reactor, fermentation, directly to adding cellulase, lignocellulosic material and Pediococcus acidilactici Pediococcus acidilactici TY112 (CGMCC 8664) to synchronize saccharification and fermentation or substep saccharification and fermentation production of L-lactic acid in fermentation reactor, it is not required to keep germ-free condition in lactic acid production process.
Lactic acid bacteria used of the invention is Pediococcus acidilactici Pediococcus acidilactici TY112, CGMCCNo.8664；The depositary institution of the Pediococcus acidilactici is China Microbial Culture Preservation Commission's common micro-organisms center (CGMCC)；Depositary institution address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Preservation date is on December 31st, 2013, is obtained by East China University of Science's bioreactor engineering National Key Laboratory bioenergy and biorefinery laboratory cultures.
Compared with prior art, the present invention has the advantage that as follows with effect：
1st, the present invention carries out open synchronous saccharification and fermentation production of L-lactic acid suitable for lignocellulosic material.
2nd, the present invention can be greatly reduced in lactic fermentation process the cost for sterilizing and sterilizing.
【Brief description of the drawings】
The conditional curve of maize straw synchronous saccharification and fermenting lactic acid under the solids content of Fig. 1 implementation methods 130%
The conditional curve of the open synchronous saccharification of maize straw and fermenting lactic acid under the solids content of Fig. 2 implementation methods 230%
【Specific embodiment】
Production technology of the invention includes：A kind of open production method of lectic acid of the lactic acid bacteria based on lactic acid producing pediocin, it is characterised in that concretely comprise the following steps, (1) pre-processes to lignocellulosic material；(2) mortifier produced in removing lignocellulosic preprocessing process；(3) without being sterilized with nutrients to lignocellulosic material, fermentation reactor, fermentation, directly to adding cellulase, lignocellulosic material and Pediococcus acidilactici Pediococcus acidilactici TY112 (CGMCC 8664) to synchronize saccharification and fermentation or substep saccharification and fermentation production of L-lactic acid in fermentation reactor, it is not required to keep germ-free condition in lactic acid production process.
Embodiment 1
Maize straw is carried out into dry method dilute acid pretreatment, pretreatment condition is：The solid-to-liquid ratio 2 of stalk solid and dilute sulfuric acid:1 (mass ratio), sulfuric acid dosage 2.5g/100g stalks, 175 DEG C, 50rpm is processed 5 minutes, and stalk solids content is 50% or so after pretreatment；Pretreated stalk is carried out into solid-state biological detoxication, detoxification condition is inoculum concentration 10% (volume ratio, virus-free strain is Amorphotheca resinae ZN1), 28 DEG C, 5 days, the inhibitor contents such as furfural, hydroxymethylfurfural, acetic acid were below 0.5g/L in pretreated straw after detoxification.By the maize straw after detoxification, fermentation all carries out disinfecting action with nutritive salt, fermentation tank, then the stalk after detoxification is put into 5 liters of reactors with ribbon agitating paddle carries out pre- enzymolysis, operating condition is solids content 30% (mass ratio), 50 DEG C, cellulase consumption 15FPU/g dry solids, pH value is 4.8,150rpm, liquefies 6 hours；Then 42 DEG C are cooled the temperature to, fermentation nutrients is added, Pediococcus acidilactici Pediococcus acidilactici TY112 is accessed with the inoculum concentration of 10% (volume ratio), synchronize saccharification with lactic fermentation synchronous saccharification and fermentation 66 hours.The aseptic of system in strict guarantee synchronous saccharification and fermentation process, Pfansteihl concentration is up to 97.13g/L.
Embodiment 2
Maize straw is carried out into dry method dilute acid pretreatment, pretreatment condition is：The solid-to-liquid ratio 2 of stalk solid and dilute sulfuric acid:1 (mass ratio), sulfuric acid dosage 2.5g/100g stalks, 175 DEG C, 50rpm is processed 5 minutes, and stalk solids content is 50% or so after pretreatment；Pretreated stalk is carried out into solid-state biological detoxication, detoxification condition is inoculum concentration 10% (volume ratio, virus-free strain is Amorphotheca resinae ZN1), 28 DEG C, 5 days, the inhibitor contents such as furfural, hydroxymethylfurfural, acetic acid were below 0.5g/L in pretreated straw after detoxification.Without to the maize straw after detoxification, fermentation disinfecting action is carried out with nutritive salt, fermentation tank etc., then the stalk after detoxification is put into 5 liters of reactors with ribbon agitating paddle carries out pre- enzymolysis, operating condition is solids content 30% (mass ratio), 50 DEG C, cellulase consumption 15FPU/g dry solids, pH value is 4.8,150rpm, is liquefied 6 hours；Then 42 DEG C are cooled the temperature to, fermentation nutrients is added, Pediococcus acidilactici Pediococcus acidilactici TY112 is accessed with the inoculum concentration of 10% (volume ratio), synchronize saccharification with lactic fermentation synchronous saccharification and fermentation 66 hours.Without control system it is germ-free condition in synchronous saccharification and fermentation process.Pfansteihl concentration can reach 69.38% up to 97.34g/L, the conversion ratio of cellulose.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, without departing from the inventive concept of the premise; some improvements and modifications can also be made, these improvements and modifications also should be regarded as in protection scope of the present invention.
Claims (6)
1. a kind of open production method of lectic acid of the lactic acid bacteria based on lactic acid producing pediocin, it is characterised in that concretely comprise the following steps, (1)
Lignocellulosic material is pre-processed；(2) mortifier produced in removing lignocellulosic preprocessing process；(3) need not
Lignocellulosic material, fermentation reactor, fermentation are sterilized with nutrients, directly to adding cellulose in fermentation reactor
Enzyme, lignocellulosic material and Pediococcus acidilactici Pediococcus acidilactici TY112 synchronize saccharification with fermentation or substep
Saccharification and fermentation production of L-lactic acid, are not required to keep germ-free condition in lactic acid production process.
2. a kind of open production method of lectic acid of lactic acid bacteria based on lactic acid producing pediocin according to claim 1, its feature
It is that in open lactic fermentation process, the fermentative microorganism for using is Pediococcus acidilactici Pediococcus acidilactici
TY112, preservation is registered in China Microbial Culture Preservation Commission's common micro-organisms center (CGMCC), and preservation date is
On December 31st, 2013, deposit number is CGMCCNo.8664.The bacterium can secrete pediocin, with to many
Plant the antibiotic property of microorganism.
3. a kind of open production method of lectic acid of lactic acid bacteria based on lactic acid producing pediocin according to claim 1, its feature
The lactic acid for being Pediococcus acidilactici Pediococcus acidilactici TY112 productions is Pfansteihl.
4. a kind of open production method of lectic acid of lactic acid bacteria based on lactic acid producing pediocin according to claim 1, its feature
It is in fermentation operation, without being sterilized with nutrients to lignocellulosic material, fermentation reactor, fermentation, to rely on breast
The pediocin of sour piece coccus Pediococcus acidilactici TY112 itself secretions avoids lactic acid to the suppression of microorganism
In fermentation process miscellaneous bacteria infects, it is ensured that lactic fermentation process is normally carried out.
5. a kind of open production method of lectic acid of lactic acid bacteria based on lactic acid producing pediocin according to claim 1, its feature
Be the method that is pre-processed to lignocellulosic include dry method dilute acid pretreatment, dilute acid pretreatment, steam blasting pretreatment,
The swollen quick-fried pretreatment of ammonia fiber, ammoniacal liquor extraction pretreatment, neutral hot-water pretreatment, ionic liquid pretreatment and other preprocess methods.
6. a kind of open production method of lectic acid of lactic acid bacteria based on lactic acid producing pediocin according to claim 1, its feature
Be mortifier in lignocellulosic after pre-processing removal methods include biodegradation, washing, activated carbon, cross alkalize adjust,
Electrodialysis and other mortifier removal methods.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108060189A (en) * | 2016-11-07 | 2018-05-22 | 华东理工大学 | A kind of maize straw dilute acid pretreatment method for reducing reactor corrosion degree |
| CN109251938A (en) * | 2017-07-13 | 2019-01-22 | 华东理工大学 | A kind of Pediococcus acidilactici construction method of common fermentation glucose and xylose production Pfansteihl |
| CN112941117A (en) * | 2020-12-23 | 2021-06-11 | 华东理工大学 | Method for synthesizing L-lactide from chiral L-lactic acid produced by using lignocellulose biomass as raw material |
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| CN103103224A (en) * | 2011-11-10 | 2013-05-15 | 华东理工大学 | Method for producing high-concentration lactic acid by lignocellulose at high temperature |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103103224A (en) * | 2011-11-10 | 2013-05-15 | 华东理工大学 | Method for producing high-concentration lactic acid by lignocellulose at high temperature |
Non-Patent Citations (2)
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| GANG LIU等: "High titer L-lactic acid production from corn stover with minimum", 《BIORESOURCE TECHNOLOGY》 * |
| QURESHI ABDUL SATTAR（萨塔）: "农作物秸秆木质纤维素生产乙醇和乳酸的高效生物炼制过程研究", 《中国博士学位论文全文数据库 工程科技I辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108060189A (en) * | 2016-11-07 | 2018-05-22 | 华东理工大学 | A kind of maize straw dilute acid pretreatment method for reducing reactor corrosion degree |
| CN109251938A (en) * | 2017-07-13 | 2019-01-22 | 华东理工大学 | A kind of Pediococcus acidilactici construction method of common fermentation glucose and xylose production Pfansteihl |
| CN109251938B (en) * | 2017-07-13 | 2022-08-23 | 华东理工大学 | Construction method of pediococcus acidilactici for producing L-lactic acid by co-fermenting glucose and xylose |
| CN112941117A (en) * | 2020-12-23 | 2021-06-11 | 华东理工大学 | Method for synthesizing L-lactide from chiral L-lactic acid produced by using lignocellulose biomass as raw material |
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