CN105645587B - The biological agent of industrial pollution water body processing - Google Patents
The biological agent of industrial pollution water body processing Download PDFInfo
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- CN105645587B CN105645587B CN201610100231.9A CN201610100231A CN105645587B CN 105645587 B CN105645587 B CN 105645587B CN 201610100231 A CN201610100231 A CN 201610100231A CN 105645587 B CN105645587 B CN 105645587B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/301—Aerobic and anaerobic treatment in the same reactor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
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Abstract
The present invention relates to a kind of biological agent of industrial pollution water body processing, it includes complex micro organism fungicide and carrier, and the complex micro organism fungicide is prepared by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier, denitrifying bacterium.Reasonable compatibility between each strain of the present invention, symbiosis are coordinated, mutually not antagonism, add in waste water treatment system, have good degradation effect to phenyl amines difficult degradation compound, suitable for the processing of industrial pollution water body, processing water and water quality treatment are improved, reduces operating cost, promotes qualified discharge.
Description
Technical field
The invention belongs to microbial technology field, and in particular to a kind of biological agent for the processing of industrial pollution water body.
Background technology
With urban population increasingly expansion and industrial or agricultural continuous development, water environment pollution frequent accident, seriously
The health or even life that compromise people, animal.Many lakes and reservoir because nitrogen, phosphorus discharge caused by body eutrophication, seriously
Threaten the production and living and the ecological balance of the mankind.Industrial pollution aqueous body compositions are complicated, particularly substantial amounts of artificial synthesized chemical combination
Thing enters environment, and this kind of material is mainly alkanes, olefines, alicyclic hydrocarbon type, arene, artificial synthesized organic matter, especially
It is macromolecular, difficult degradation, poisonous and harmful substance such as phenol, chlorophenol, cresols, nitrophenols etc., arene, cyanogen class, amine with
And ammonia nitrogen etc. enters environment with the waste water or solid waste of plant emissions, due to the complexity and biology of these materials this body structures
Strange property, cannot be decomposed by the microorganisms utilization in a short time, traditional wastewater treatment method activated sludge culture
Microorganism effectively cannot remove these pollutants, these materials accumulate in the environment for a long time, rely life to us
The ecological environment deposited causes very big pollution, and the physical and mental health to the mankind brings very big harm.Quite a few industrial pollution of China
Enterprise, which would rather be punished, is also unwilling that waste water is administered in investment, and even if having, sewage-treatment plant operation is also abnormal.Therefore, one is developed
Kind construction investment is few, operating cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
And national defence, printing and dyeing, plastics, paint, pesticide and medical industry etc. obtain the presence for having aniline category matter in industrial wastewater,
Aniline is the most representative material of aromatic amine, is a kind of colourless oil liquid with aromatic odor, while is also serious
Pollution environment and the harmful substance being detrimental to health, are that the three of a kind of " carcinogenic, teratogenesis, mutagenesis " cause material.Due to aniline
There is extended residual, bioconcentration, carcinogenicity, 129 kinds of pollutants of priority acccess control are classified as by U.S. EPA,
It is put into " Chinese environmental priority pollutant blacklist ", stringent control is required in industrial water drainage.Handle the biography of aniline waste water
System method mainly has the methods of physics, chemistry, biology.
Process for treating industrial waste water mainly has reverse osmosis physical method, distillation, soil to irrigate at present；Chemical method has ion friendship
Change method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatments, catalytic pyrolysis；Biological method has nitrification and algae
Class cultivates, but physical method treatment effect is bad, and than chemical method, biological method processing waste water has the following advantages：1) it is every kind of
Chemical article is possible to fail, and biological agent is to dirt both for the very strong product of property when running into other chemical substances
The removal of dye thing has spectrum；2) chemical products can temporarily eliminate some harmful substances and cover stink, cannot but hinder
The only generation of harmful substance；3) after using chemical products, residual is had in water body, secondary pollution may be caused.Biological agent institute
Containing natural microbial, without pathogenic bacteria and pathogen, these microorganisms are under the catalytic action of enzyme, with the organic nutrition in sewage
Material is food, and after purifying the sewage, these microbes are gradually decreased with the reduction of pollutant, until withering away；4)
Nontoxic, non-corrosiveness is easy to use, need not add equipment or engineering substantially, saves fund input.
Bioremediation is to administer the effective ways of industrial pollution water body.It is the effect using microorganism, makes chemical combination
Thing is decomposed.Since bioremediation is more much lower than physics, chemical method cost, and non-secondary pollution, and microorganism is again
With stronger variability and adaptability, therefore it has become the ideal method of processing industrial pollution water body.
The content of the invention
The object of the present invention is to provide a kind of biological agent for the processing of industrial pollution water body.
Contain aniline in the industrial pollution water body.
The biological agent of the present invention, reasonable compatibility between each strain, symbiosis are coordinated, mutually not antagonism, and its preparation method is easy,
Easy to implement the method, its is easy to operate, beneficial to production.
The present invention adopts the following technical scheme that realization：
The biological agent of industrial pollution water body processing, which includes complex micro organism fungicide and carrier, described multiple
Microbial bacterial agent is closed by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, denitrogenation pair
It is prepared by coccus, nitrifier, denitrifying bacterium.
The carrier can be solid carrier and/or liquid-carrier.The solid carrier for mineral material, biomaterial and/
Or high-molecular compound；The mineral material can be clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica, turfy soil
At least one of with diatomite；The biomaterial is the stalk of all kinds of crops, loose shell, straw, peanut shell, corn flour, beans
At least one of powder, starch, turf and excrement of animal；The high-molecular compound is polyvinyl alcohol and/or polyglycols.Institute
It can be organic solvent, vegetable oil, mineral oil or water to state liquid-carrier；The organic solvent is decane and/or dodecane.It is described viscous
Native concretely attapulgite clay.The attapulgite clay is a kind of clay mineral using attapulgite as main component, mainly
Chemical composition is hydration zeopan, containing elements such as K, Na, Ca, Fe, Al, Mg, Mn, Ti, has unique dispersibility
And adsorption capacity.
By verification, final choice carrier is made of following raw materials：Diatomite, turfy soil.
Preferably, the carrier by diatomite, turfy soil according to weight ratio 4：1 mixes；
The complex micro organism fungicide and carrier are according to 1：3 weight ratio is mixed with biological agent；
The preparation method of the complex micro organism fungicide is as follows：By pseudomonas putida, candidiasis, solution starch gemma
It is 1 × 10 that bacillus, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier, denitrifying bacterium are cultivated to concentration respectively8A/ml
Bacterium solution, then according to 5：2：4：2：3：2：2:1 volume ratio mixing, to obtain the final product；
Preferably：
The pseudomonas putida is pseudomonas putida（Pseudomonas putida）CCTCC 203021
（CN1513980 discloses use）；
The candidiasis are candidiasis（Candida utilis）ATCC No.22023;
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The Clostridium papyrosolvens are（Clostridium papyrosolvens）ATCC 700395；
The nitrococcus is nitrococcus（Nitrosomonas europaea）CCTCC No：M 2010002
（CN101955885）；
The Paracoccus denitrificans concretely Paracoccus denitrificans (Paracoccus denitrificans) ATCC13543；
The nitrifier is (Nitrobacter sp.) CCTCC 2010001 (CN101955885)；
The denitrifying bacterium is denitrifying bacterium（Paracoccus pantotrophus）ATCC 35512；
Strain of the present invention can be from China Committee for Culture Collection of Microorganisms's common micro-organisms center
(CGMCC) and American Type Culture collection warehousing（ATCC）, Chinese agriculture Microbiological Culture Collection administrative center（ACCC）Purchase
It can buy.
Those skilled in the art can select suitable culture medium according to general knowledge and expand cultural method, reach viable count
108A/gram, and prepared according to the conventional method for preparing microbial solid preparation.
The various strains that can form dominant microflora are configured to high-efficiency microorganism preparation, by one by the composite bacteria agent of the present invention
Quantitatively it is added in waste water treatment system, accelerates the degraded of microbe, to improve the biological treatment efficiency of system, protects
Demonstrate,prove system stable operation.It contains a variety of microorganisms for having excellent degradation capability to Recalcitrant chemicals, between each strain rationally
Compatibility, symbiosis are coordinated, mutually not antagonism, and activity is high, and biomass is big, and breeding is fast, adds in waste water treatment system, dirty to phenyl amines
Dye water body has good degradation effect, suitable for Industrial Waste Water Treatments, can improve processing water and water quality treatment, reduce running cost
With promotion qualified discharge.
Embodiment
Embodiment 1
The biological agent of industrial pollution water body processing, the microbial inoculum include following raw materials：Complex micro organism fungicide and carrier, institute
Complex micro organism fungicide is stated by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, de-
It is prepared by nitrogen pair coccus, nitrifier, denitrifying bacterium.
The carrier is by diatomite, turfy soil according to weight ratio 4：1 mixes；
The composite bacteria agent and carrier are according to 1：3 weight ratio is mixed with biological agent；
The preparation method of the complex micro organism fungicide is as follows：By pseudomonas putida, candidiasis, solution starch gemma
It is 1 × 10 that bacillus, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier, denitrifying bacterium are cultivated to concentration respectively8A/ml
Bacterium solution, then according to 5：2：4：2：3：2：2:1 volume ratio mixing, to obtain the final product；
Preferably：
The pseudomonas putida is pseudomonas putida（Pseudomonas putida）CCTCC 203021
（CN1513980 discloses use）；
The candidiasis are candidiasis（Candida utilis）ATCC No.22023;
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The Clostridium papyrosolvens are（Clostridium papyrosolvens）ATCC 700395；
The nitrococcus is nitrococcus（Nitrosomonas europaea）CCTCC No：M 2010002
（CN101955885）；
The Paracoccus denitrificans concretely Paracoccus denitrificans (Paracoccus denitrificans) ATCC13543；
The nitrifier is (Nitrobacter sp.) CCTCC 2010001 (CN101955885)
The denitrifying bacterium is denitrifying bacterium（Paracoccus pantotrophus）ATCC 35512；
Strain of the present invention can be from China Committee for Culture Collection of Microorganisms's common micro-organisms center
(CGMCC) and American Type Culture collection warehousing（ATCC）, Chinese agriculture Microbiological Culture Collection administrative center（ACCC）Purchase
It can buy.
Those skilled in the art can select suitable culture medium according to general knowledge and expand cultural method, reach viable count
108A/gram, and prepared according to the conventional method for preparing microbial solid preparation.
Embodiment 2
In certain sewage treatment plant, by the use of 50L buckets as testing equipment and with stirring, three 30L of blast pool water outlet are taken, are divided
Jia Ru not be in two buckets, it is 7.0 to adjust pH, and 20 DEG C of water temperature, is measured by sampling COD, ammonia nitrogen, total nitrogen data；Before processing COD is 123mg/
L, ammonia nitrogen 37mg/L；Test group addition embodiment 1 prepare biological agent 10g stirring 1 it is small when, stop stirring 3 it is small when after take
Sample measure COD, ammonia nitrogen data；The water of equivalent weight is added in blank control, operates same test group.Concrete outcome is shown in Table 1：
Table 1
| COD average removal rates | Ammonia nitrogen average removal rate | |
| Blank control group | 7% | 10% |
| Test group | 92% | 91% |
Embodiment 3
The biological agent prepared using embodiment 1 handles certain chemical plant industrial wastewater, and treatment process is A/O+ precipitations+aeration
Biological filter, A/O ponds 20 grams of biological agent as made from every cubic metre of biochemical pool volume adds embodiment 1 every time, adds 1 daily
It is secondary, continuously add 10 days；Biological aerated filter is added 20 grams of the biological agent of the preparation of embodiment 1 by every cubic meter filler, is thrown daily
Add 1 time, continuously add 10 days；After one month, treatment effect such as following table：
Although above detailed explanation is made to this case with generality explanation and embodiment, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
The modification or improvement made without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (5)
1. industrial pollution water body processing biological agent, it is characterised in that the biological agent include complex micro organism fungicide and
Carrier, the complex micro organism fungicide is by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrous
It is 1 × 10 to change bacterium, Paracoccus denitrificans, nitrifier, denitrifying bacterium to cultivate respectively to concentration8The bacterium solution of a/ml, then according to 5：2：
4：2：3：2：2：1 volume ratio is obtained by mixing.
2. biological agent according to claim 1, it is characterised in that the carrier is by diatomite and turfy soil according to weight
Than 4：1 mixes.
3. biological agent according to claim 1 or 2, it is characterised in that the complex micro organism fungicide and carrier according to
1：3 weight ratio is mixed with biological agent.
4. biological agent according to claim 1, it is characterised in that：
The pseudomonas putida is pseudomonas putida（Pseudomonas putida）CCTCC No：203021；
The candidiasis are candidiasis（Candida utilis）ATCC 22023;
The bacillus amyloliquefaciens are bacillus amyloliquefaciens（Bacillus amyloliquefaciens）ATCC 23843；
The Clostridium papyrosolvens are Clostridium papyrosolvens（Clostridium papyrosolvens）ATCC 700395；
The nitrococcus is nitrococcus（Nitrosomonas europaea）CCTCC No：M 2010002；
The Paracoccus denitrificans for Paracoccus denitrificans (Paracoccus denitrificans) ATCC 13543；
The nitrifier for nitrifier (Nitrobacter sp.)CCTCC No：2010001；
The denitrifying bacterium is denitrifying bacterium（Paracoccus pantotrophus）ATCC 35512.
5. the biological agent described in claim 1 or 4 is used for the purposes of industrial pollution water body processing.
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| CN106754543A (en) * | 2017-01-11 | 2017-05-31 | 王筠涵 | A kind of microorganism formulation for sludge anaerobic fermentation |
| CN108101228A (en) * | 2017-12-13 | 2018-06-01 | 浙江海洋大学 | A kind of wastewater treatment microbial microbial inoculum |
| CN108641985B (en) * | 2018-05-21 | 2021-04-09 | 万创青绿（北京）环境科技有限公司 | Composite microbial purifying agent for purifying river sewage and preparation method thereof |
| CN109576189B (en) * | 2018-12-29 | 2022-04-19 | 合肥市东方美捷分子材料技术有限公司 | Composite microbial inoculum for black and odorous water body and preparation method and application thereof |
| CN110923184A (en) * | 2019-12-01 | 2020-03-27 | 齐齐哈尔龙江阜丰生物科技有限公司 | Biological agent for repairing threonine fermentation wastewater |
| CN113213694A (en) * | 2020-12-17 | 2021-08-06 | 云南中贸环境节能科技投资股份有限公司 | Microbial carrier system for sewage treatment and process method |
| CN112795560A (en) * | 2020-12-24 | 2021-05-14 | 天津国瑞蓝天科技有限公司 | Biological agent for treating industrial wastewater and preparation method thereof |
| CN112725242B (en) * | 2021-02-10 | 2023-12-05 | 杭州楠大环保科技有限公司 | Special microbial inoculum for strengthening sewage centralized treatment of activated sludge and preparation method thereof |
| CN112852674B (en) * | 2021-02-20 | 2022-08-23 | 杭州楠大环保科技有限公司 | Preparation method of industrial sewage treatment microbial inoculum |
| CN117904011B (en) * | 2024-03-20 | 2024-05-28 | 江苏省环境工程技术有限公司 | Microbial agent for treating high-salt refractory industrial wastewater and preparation method and application thereof |
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