CN104694447A - Complex microbial inoculum for treating sewage and application method thereof - Google Patents

Complex microbial inoculum for treating sewage and application method thereof Download PDF

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CN104694447A
CN104694447A CN201510157233.7A CN201510157233A CN104694447A CN 104694447 A CN104694447 A CN 104694447A CN 201510157233 A CN201510157233 A CN 201510157233A CN 104694447 A CN104694447 A CN 104694447A
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microbial inoculum
sewage
subtilis
saccharomyces cerevisiae
pseudomonas aeruginosa
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朱凌玮
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds

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Abstract

The invention relates to an improved biological microbial inoculum for treating industrial sewage and a preparation method and application thereof. The microbial inoculum is thrown in a sewage treating system, has a good degrading effect on macromolecular, degradation-resistant, toxic and harmful substances such as arsenic, ammonia nitrogen and phosphorus and has a unique treatment effect on high-concentration organic sewage and high-ammonia-nitrogen sewage which are difficult for a traditional activated sludge method to treat.

Description

A kind of composite fungus agent of disposing of sewage and application method thereof
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of bacteria agent of disposing of sewage and preparation method and application.
Background technology
Underground water is the important drinking water sources in the many areas of China.But along with work, rural activity, the groundwater pollution of China's major part plains region is very serious, and the simultaneous combined pollution of multiple pollutant often, wherein heavy metal and nitrate combined pollution are modal combined pollutions.Show according to the monitoring data of 20 years, the multiple pollutant such as nitrate in groundwater, sexavalent chrome of Xi'an exceeds standard for a long time.
Microorganism is the same with animals and plants, be one of large species of nature three, and in whole ecological recycle system, microorganism is the decomposer of plant and animal residues organic waste.Therefore, microorganism plays very important role, i.e. a processor for sewage and rubbish in ecological circulation and field of Environment Protection.Nearly all sewage disposal is all lean on the effect of microorganism to complete, and in sewage and filth disposal, not only needs microbial decomposition and remove various objectionable impurities, also will carry out deodorizing by microorganism.And the kind of microorganism and function and quantity determine processing speed and the treatment effect of sewage and dirt to a great extent.
Traditional sewage water treatment method is the techniques such as precipitation, anaerobism, aerobic aeration, and function yeast used takes from natural active sludge, is namely carried out the organic waste in degradation of sewage by the anaerobic-aerobic microorganism that nature is natural.The outlet effect of traditional waste water treatment process usually seems unstable, the secondary pollution problem that the same problem that nearly all sewage work faces is exactly post-depositional sludge disposal and brings by the impact of environmental factors (weather, season, sewage different sources etc.).In addition, the process of some special source sewage is still needed and will be taked special chemical means, and this pollution for environment is more serious.
In prior art, such as CN102888374A, discloses a kind of effectively compound microbial inoculant for Industrial Waste Water Treatments and utilizes high efficiency composition microbial inoculum, which using prunosus red coccus, subtilis, Pseudomonas aeruginosa, yeast saccharomyces cerevisiae, Nitrosomonas europaea, Bacillus megaterium, aspergillus niger, several bacterium such as Paracoccus denitrificans has prepared microbial inoculum, demonstrates its effect.But this microbial inoculum is in dephosphorization and go to have certain weak tendency above the removal effect of COD and duplicate removal metal, therefore, applicant is to this has been the microbial inoculum improving and obtain better effects if.In view of the above problems, be necessary that cultivating a kind of sewage disposal high-efficiency combined functional bacteria solves the problems referred to above.
Summary of the invention
A kind of method that the object of this invention is to provide effectively compound microbial inoculant for Industrial Waste Water Treatments and utilize high efficiency composition microbial inoculum to dispose of sewage.
Sewage disposal composite fungus agent of the present invention, reasonable compatibility between each bacterial classification, symbiosis is coordinated, mutually not antagonism, and its preparation method is easy, easy to implement the method, and it is easy and simple to handle, is beneficial to production.
The present invention adopts following technical scheme to realize:
Scheme 1: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Listera 8%, Erwinia 11%, prunosus red coccus 17%, subtilis 14%, Pseudomonas aeruginosa 7%, yeast saccharomyces cerevisiae 8%, Nitrosomonas europaea 10%, aspergillus niger 14%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 6%.
Scheme 2: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Rhodopseudomonas spheroides 8%, Erwinia 12%, prunosus red coccus 17%, subtilis 15%, Pseudomonas aeruginosa 12%, yeast saccharomyces cerevisiae 11%, bacillus cereus 13%, aspergillus niger 4%, Paracoccus denitrificans 3%, Fe forms denitrifying bacteria 5%.
Scheme 3: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Rhodopseudomonas spheroides 8%, citrobacter 10%, prunosus red coccus 13%, subtilis 17%, Pseudomonas aeruginosa 10%, yeast saccharomyces cerevisiae 13%, Nitrosomonas europaea 8%, bacillus cereus 7%, aspergillus niger 6%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 3%.
Scheme 4: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Shigellae 9%, citrobacter 14%, prunosus red coccus 15%, subtilis 13%, Pseudomonas aeruginosa 11%, yeast saccharomyces cerevisiae 9%, Nitrosomonas europaea 7%, aspergillus niger 6%, Paracoccus denitrificans 10%, Fe forms denitrifying bacteria 6%.
Scheme 5: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Shigellae 8%, citrobacter 14%, prunosus red coccus 13%, subtilis 11%, Pseudomonas aeruginosa 9%, yeast saccharomyces cerevisiae 9%, glutinous rhodotorula 6%, Nitrosomonas europaea 7%, aspergillus niger 7%, Paracoccus denitrificans 9%, Fe forms denitrifying bacteria 7%.
Bacterial classification of the present invention all can from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), China typical culture collection center and American Type Culture collection warehousing (ATCC), Chinese agriculture Microbiological Culture Collection administrative center (ACCC) buy and obtain.
The existence form of composite fungus agent of the present invention is preferably the mixture of lyophilized powder, microbial inoculum or microbial inoculum and solid adjuvant material.When the existence form of described microbial inoculum is lyophilized powder, preparation method comprises and described bacterium is prepared fermented liquid respectively, after mass ratio mixing, makes lyophilized powder by this area ordinary method.
Wherein each strain fermentating liquid preparation process is:
1, the independent enlarged culturing of various bacterial classification:
Fe forms denitrifying bacteria: be seeded in by Fe forms denitrifying bacteria on inclined-plane solid medium, its component is: C6H5Na3O7CH2O 5g/L, K2HPO4 Ig/L, KH2PO40.5g/L, MgSO4.7H20 0.2g/L, KNO3 2g/L, FeCl2.4H20 0.998g/L; 30 ° of C cultivate; Then, proceed in liquid nutrient medium, component is for being C6H5Na3O7.2H20 5g/L, K2HPO4 I g/L, KH2PO4 0.5g/L, MgSO4.7H20 0.2g/L, KNO3 2g/L, FeCl2.4H20 0.998g/L; 30 DEG C of 220r/min cultivate, to the dense 3.0-4.0X I0 of bacterium 8individual/gram.
Listera: Listera bacterial classification is seeded in respectively on nutrient broth (agar) the substratum nutrient broth medium (with reference to microbiology test textbook) containing 3% glycerine, 28-30 DEG C, make primary inclined plane to cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 2.0-5.0X I0 8individual/gram.
Erwinia, bacillus cereus, citrobacter, Shigellae, subtilis, the cultivation of bacillus megaterium: bacterium test tube kind be first seeded on beef-protein medium, 28-30 DEG C, makes primary inclined plane and cultivates, then be inoculated in triangular flask and do the cultivation of vibration secondary liquid, then proceed to liquid fermentation tank and do three grades of liquid culture, be finally inoculated on solid medium and do level Four cultivation, to product, viable count reaches 2.0-4.0X I0 8individual/gram.
The cultivation of rhodococcus, Paracoccus denitrificans: rhodococcus, Paracoccus denitrificans bacterial classification are seeded on nutrient broth medium (with reference to microbiology test textbook) respectively, 28-30 DEG C, make primary inclined plane to cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 2.0-5.0X I0 8individual/gram.
The cultivation of yeast saccharomyces cerevisiae, glutinous rhodotorula, aspergillus niger: first yeast saccharomyces cerevisiae, aspergillus niger are seeded on potato dextrose agar (PDA), 28-30 DEG C, make primary inclined plane to cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0X I0 8individual/gram.
Pseudomonas aeruginosa is cultivated: Pseudomonas aeruginosa is first on substratum, and 28-30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0X I0 8individual/gram, described medium component is: NH4Cl 1.0g, CH3C00Na3.5g, MgCl20.lg, CaCl20.lg, KH2PO40.6g, K2HPO40.4g, yeast extract paste 0.lg, total amount 1000ml, pH7.2.
The cultivation of nitrococcus: nitrococcus is first on substratum, and 28_30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0X I0 8individual/gram, described medium component is: 2% glucose (w/v), 1.5% extractum carnis (w/v), 1.5% peptone (w/v), 0.058% magnesium sulfate (w/v), 0.025% manganous sulfate (w/v), 0.22% sodium acetate (w/v), 0.2% dipotassium hydrogen phosphate (w/v), solvent is water;
The kind microbial inoculum cultivated by above bacterium is uniformly mixed by described mass ratio, preferably with diatomite (40-80 order) for carrier, be uniformly mixed according to the weight ratio of 1:4 and microbial strain culture.
Dry: will mix material and carry out drying, drying temperature is 20-50 ° of C, after dry, water content is 20-30%;
Inspection, packaging: by quality standard inspection, finished product is packed by weight, to obtain final product.
Note, in above-mentioned steps, strain expanded culture and the method for preparing solid fungicide are not unique, and those skilled in the art can select suitable substratum and enlarged culturing method according to general knowledge, make viable count reach 10 8individual/gram, and conveniently prepare the method preparation of solid fungicide.
Composite fungus agent of the present invention is by the various bacterial classification that can form dominant microflora, be mixed with high-efficiency microorganism preparation, be added in Waste Water Treatment by a certain amount of, accelerate the degraded of microbe, have better removal effect high phosphorus, COD and ammonia nitrogen and heavy metal contamination.Be suitable for Industrial Waste Water Treatments, the process water yield and water quality treatment can be improved, reduce working cost, promote qualified discharge.
Embodiment
Embodiment 1: the preparation of composite fungus agent
Described microbial inoculum forms according to concrete proportional arrangement given below.
Reference examples 1: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum comprises the raw material of following weight percents: prunosus red coccus 25%, subtilis 20%, Pseudomonas aeruginosa 15%, yeast saccharomyces cerevisiae 10%, Nitrosomonas europaea 10%, Bacillus megaterium 10%, aspergillus niger 5%, Paracoccus denitrificans 5%.
Reference examples 2: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum comprises the raw material of following weight percents: prunosus red coccus 20%, subtilis 17%, Pseudomonas aeruginosa 14%, yeast saccharomyces cerevisiae 10%, Nitrosomonas europaea 10%, Bacillus megaterium 10%, aspergillus niger 5%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 9%.
Scheme 1: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Listera 8%, Erwinia 12%, prunosus red coccus 20%, subtilis 15%, Pseudomonas aeruginosa 7%, yeast saccharomyces cerevisiae 8%, Nitrosomonas europaea 10%, aspergillus niger 15%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 6%.
Scheme 2: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Rhodopseudomonas spheroides 8%, Erwinia 12%, prunosus red coccus 17%, subtilis 15%, Pseudomonas aeruginosa 12%, yeast saccharomyces cerevisiae 11%, bacillus cereus 13%, aspergillus niger 4%, Paracoccus denitrificans 3%, Fe forms denitrifying bacteria 5%.
Scheme 3: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Rhodopseudomonas spheroides 8%, citrobacter 10%, prunosus red coccus 13%, subtilis 17%, Pseudomonas aeruginosa 10%, yeast saccharomyces cerevisiae 13%, Nitrosomonas europaea 8%, bacillus cereus 7%, aspergillus niger 6%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 3%.
Scheme 4: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Shigellae 9%, citrobacter 14%, prunosus red coccus 15%, subtilis 13%, Pseudomonas aeruginosa 11%, yeast saccharomyces cerevisiae 9%, Nitrosomonas europaea 7%, aspergillus niger 6%, Paracoccus denitrificans 10%, Fe forms denitrifying bacteria 6%.
Scheme 5: a kind of bacteria agent processing industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Shigellae 8%, citrobacter 14%, prunosus red coccus 13%, subtilis 11%, Pseudomonas aeruginosa 9%, yeast saccharomyces cerevisiae 9%, glutinous rhodotorula 6%, Nitrosomonas europaea 7%, aspergillus niger 7%, Paracoccus denitrificans 9%, Fe forms denitrifying bacteria 7%.
。
Bacterial classification of the present invention all can be bought and obtain from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) and American Type Culture collection warehousing (ATCC), Chinese agriculture Microbiological Culture Collection administrative center (ACCC).
Wherein each strain fermentating liquid preparation process is:
1, the independent enlarged culturing of various bacterial classification:
Fe forms denitrifying bacteria: be seeded in by Fe forms denitrifying bacteria on inclined-plane solid medium, its component is: C6H5Na3O7CH2O 5g/L, K2HPO4 Ig/L, KH2PO40.5g/L, MgSO4.7H20 0.2g/L, KNO3 2g/L, FeCl2.4H20 0.998g/L; 30 ° of C cultivate; Then, proceed in liquid nutrient medium, component is for being C6H5Na3O7.2H20 5g/L, K2HPO4 I g/L, KH2PO4 0.5g/L, MgSO4.7H20 0.2g/L, KNO3 2g/L, FeCl2.4H20 0.998g/L; 30 DEG C of 220r/min cultivate, to the dense 3.0-4.0X I0 of bacterium 8individual/gram.
Listera: Listera bacterial classification is seeded in respectively on nutrient broth (agar) the substratum nutrient broth medium (with reference to microbiology test textbook) containing 3% glycerine, 28-30 DEG C, make primary inclined plane to cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 2.0-5.0X I0 8individual/gram.
Erwinia, bacillus cereus, citrobacter, Shigellae, subtilis, the cultivation of bacillus megaterium: bacterium test tube kind be first seeded on beef-protein medium, 28-30 DEG C, makes primary inclined plane and cultivates, then be inoculated in triangular flask and do the cultivation of vibration secondary liquid, then proceed to liquid fermentation tank and do three grades of liquid culture, be finally inoculated on solid medium and do level Four cultivation, to product, viable count reaches 2.0-4.0X I0 8individual/gram.
The cultivation of rhodococcus, Paracoccus denitrificans: rhodococcus, Paracoccus denitrificans bacterial classification are seeded on nutrient broth medium (with reference to microbiology test textbook) respectively, 28-30 DEG C, make primary inclined plane to cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 2.0-5.0X I0 8individual/gram.
The cultivation of yeast saccharomyces cerevisiae, glutinous rhodotorula, aspergillus niger: first yeast saccharomyces cerevisiae, aspergillus niger are seeded on potato dextrose agar (PDA), 28-30 DEG C, make primary inclined plane to cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0X I0 8individual/gram.
Pseudomonas aeruginosa is cultivated: Pseudomonas aeruginosa is first on substratum, and 28-30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0X I0 8individual/gram, described medium component is: NH4Cl 1.0g, CH3C00Na 3.5g, MgCl2 0.lg, CaCl20.lg, KH2PO4 0.6g, K2HPO4 0.4g, yeast extract paste 0.lg, total amount 1000ml, pH7.2.
The cultivation of nitrococcus: nitrococcus is first on substratum, and 28_30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0X I0 8individual/gram, described medium component is: 2% glucose (w/v), 1.5% extractum carnis (w/v), 1.5% peptone (w/v), 0.058% magnesium sulfate (w/v), 0.025% manganous sulfate (w/v), 0.22% sodium acetate (w/v), 0.2% dipotassium hydrogen phosphate (w/v), solvent is water;
The kind microbial inoculum cultivated by above bacterium is uniformly mixed by described mass ratio, preferably with diatomite (40-80 order) for carrier, be uniformly mixed according to the weight ratio of 1:4 and microbial strain culture.
Dry: will mix material and carry out drying, drying temperature is 20-50 ° of C, after dry, water content is 20-30%;
Inspection, packaging: by quality standard inspection, finished product is packed by weight, to obtain final product.
Embodiment 2 microbial inoculum treatment effect is tested
In municipal sewage plant, utilize 50L bucket as testing installation and be with stirring, getting blast pool water outlet 30L, add in bucket, adjusting pH to be 7.0, water temperature 20 ° of C, sampling and measuring COD, ammonia nitrogen, total nitrogen, phosphorus, getting active sludge and measure heavy metal data;
In water body, before process, COD is 79mg/L, and ammonia nitrogen is 20mg/L, total nitrogen 30mg/L, total phosphorus 40mg/mL, sludge heavy-metal (Cu), (Cr), (Pb), (As) and (Cd) (butt) be respectively 40,80,121,35,16 mg/kg.
One of them bucket adds microbial inoculum 10g prepared by embodiment 1, stirs I hour, and stopping stirring is sampling and measuring C0D, ammonia nitrogen, total nitrogen data after 3 hours, and measure the content of Heavy Metals in Sludge after 24h, result is as follows:
COD average removal rate Ammonia nitrogen average removal rate Volatile phenol clearance Total tp removal rate Heavy metal removing rate
Reference examples 1 88.3% 94.1% 97.2% 70.1% 40.5%
Reference examples 2 86.2% 93.7% 96.6% 67.1% 41.9%
Scheme 1 99.2% 99.1% 99.9% 99.7% 96.5%
Scheme 2 99.3% 99.5% 99.4% 99.3% 97.3%
Scheme 3 99.0% 99.7% 99.7% 99.1% 96.4%
Scheme 4 99.2% 99.3% 99.3% 99.0% 95.3%
Scheme 5 79.2% 70.6% 81.5% 61.3% 70.2%
Embodiment 3
Adopt composite fungus agent process city factory industry waste water prepared by embodiment 1, treatment process is the filter of A/0+ precipitation+aeration and biological, A/0 pond adds the obtained composite and highly effective microorganism preparation 20 grams of embodiment 1 by every cubic metre of biochemistry pool volume at every turn, and every day adds I time, adds two weeks continuously; BAF adds this composite and highly effective microorganism preparation 40 grams by every cubic meter filler, and every day adds I time, adds 10 days continuously; After one month, its every clearance of microbial inoculum of scheme 1-4 is adopted to reach 99.9%.Reference examples gap on the removal of phosphorus and heavy metal is comparatively large, and particularly the clearance of heavy metal can only maintain about 40%.Particularly on the removal of heavy metal As, scheme 1-4 due to proportioning between each component proper, achieve good removal effect, As removes substantially completely, and also can only reach about 70% by the clearance detecting As in discovery scheme 5, the As clearance of contrast 1 and 2 also can only reach about 60%, and this also absolutely proves, adopt between each bacterial strain of specified proportion mixing and can play good complementary action, good effect can be played in sewage disposal.
With general explanation and embodiment, detailed explanation has been done to this case in literary composition, but on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.

Claims (10)

1. one kind processes the bacteria agent of industrial sewage, the activeconstituents of this microbial inoculum comprises the raw material of following weight percents: prunosus red coccus 25%, subtilis 20%, Pseudomonas aeruginosa 15%, yeast saccharomyces cerevisiae 10%, Nitrosomonas europaea 10%, Bacillus megaterium 10%, aspergillus niger 5%, Paracoccus denitrificans 5%.
2. one kind processes the bacteria agent of industrial sewage, the activeconstituents of this microbial inoculum comprises the raw material of following weight percents: prunosus red coccus 20%, subtilis 17%, Pseudomonas aeruginosa 14%, yeast saccharomyces cerevisiae 10%, Nitrosomonas europaea 10%, Bacillus megaterium 10%, aspergillus niger 5%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 9%.
3. one kind processes the bacteria agent of industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Listera 8%, Erwinia 12%, prunosus red coccus 20%, subtilis 15%, Pseudomonas aeruginosa 7%, yeast saccharomyces cerevisiae 8%, Nitrosomonas europaea 10%, aspergillus niger 15%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 6%.
4. one kind processes the bacteria agent of industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Rhodopseudomonas spheroides 8%, Erwinia 12%, prunosus red coccus 17%, subtilis 15%, Pseudomonas aeruginosa 12%, yeast saccharomyces cerevisiae 11%, bacillus cereus 13%, aspergillus niger 4%, Paracoccus denitrificans 3%, Fe forms denitrifying bacteria 5%.
5. one kind processes the bacteria agent of industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Rhodopseudomonas spheroides 8%, citrobacter 10%, prunosus red coccus 13%, subtilis 17%, Pseudomonas aeruginosa 10%, yeast saccharomyces cerevisiae 13%, Nitrosomonas europaea 8%, bacillus cereus 7%, aspergillus niger 6%, Paracoccus denitrificans 5%, Fe forms denitrifying bacteria 3%.
6. one kind processes the bacteria agent of industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Shigellae 9%, citrobacter 14%, prunosus red coccus 15%, subtilis 13%, Pseudomonas aeruginosa 11%, yeast saccharomyces cerevisiae 9%, Nitrosomonas europaea 7%, aspergillus niger 6%, Paracoccus denitrificans 10%, Fe forms denitrifying bacteria 6%.
7. one kind processes the bacteria agent of industrial sewage, the activeconstituents of this microbial inoculum is the raw material of following weight percents: Shigellae 8%, citrobacter 14%, prunosus red coccus 13%, subtilis 11%, Pseudomonas aeruginosa 9%, yeast saccharomyces cerevisiae 9%, glutinous rhodotorula 6%, Nitrosomonas europaea 7%, aspergillus niger 7%, Paracoccus denitrificans 9%, Fe forms denitrifying bacteria 7%.
8. the arbitrary described application of bacteria agent in Treating Municipal Sewage of claim 1-7.
9. apply as claimed in claim 8, it is characterized in that: in sewage, be rich in heavy metal.
10. apply as claimed in claim 9, it is characterized in that: described heavy metal comprises arsenic.
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